CN207567208U - For cultivating the microwell plate of photosynthetic microalgae - Google Patents

Abstract

The utility model belongs to microculture utensil, particularly relates to a kind of microwell plate for being used to cultivate photosynthetic microalgae.Including substrate, it is opened on substrate and in the micropore of matrix distribution, is covered on the cover board of upper surface of base plate；Cover board and substrate are made of transparent material, lid surface is equipped with the plug being adapted to and inserting cooperation can be realized with it corresponding with micropore, the adjacency section of micropore and plug is equipped with containment member, plug is equipped with strainer for hollow tubular structure and bottom, and the sterile packing with airing function is filled in the plug inner cavity on strainer top.When the utility model efficiently solves culture of the existing technology inside micropore and the technical issues of external environment gas exchanges are not smooth, with be effectively ensured photosynthetic microalgae in incubation with the gas exchanges of external environment, the advantages that existing microwell plate is overcome when cultivating photosynthetic microalgae because of edge effect caused by gas circulation difference, avoids the screening deviation because of its generation.

Description

For cultivating the microwell plate of photosynthetic microalgae

Technical field

The utility model belongs to microculture utensil, particularly relates to a kind of microwell plate for being used to cultivate photosynthetic microalgae.

Background technology

Photosynthetic microalgae has been widely used in the fields such as food, medicine, the energy, environmental protection as a kind of microbial resources, certainly Widely distributed in right environment, Strain selection is the important process for developing microalgae resource.The conventional screening assays of algae are in shaking flask Middle progress, it is time-consuming and laborious for large batch of screening.High flux screening is that one kind is opened for strain improvement and noval chemical compound The new technology of hair etc., it is that high density, fast and parallel the side for measuring high-volume sample are carried out on the basis of microwell plate Method.During strain high flux screening, the instruments such as microwell plate and continuous sample adding apparatus, the volley of rifle fire and microplate reader are used in combination, can be with The batch operation time is greatly saved, improves efficiency, this method has been used for many heterotrophic microorganism bacteria selections.Photosynthetic algae It is different from heterotrophic microorganism, illumination is needed to carry out photosynthesis in growth course, absorbing carbon dioxide simultaneously releases oxygen, so And with the common photosynthetic microalgae of microwell plate culture, there are certain restrictions.First, a large amount of oxygen can be discharged during photosynthetic type micro algae growth Gas, since microwell plate single hole volume is small and has cover board, it is poor to be exchanged with extraneous gas, and oxygen concentration in culture solution is caused to increase, for The metabolic way of microalgae is different under different oxytys for some algaes, and excessively high oxygen concentration is also possible to that frustule can be inhibited Growth.Second is that because the aperture of microwell plate is small and trepanning is densely distributed, the gas exchange capacity of orifice plate central position is worst, side The gas exchange capacity of edge position is strong, this can the edge effect caused by the difference in oxygen concentration that center to edge generates.Medium pore culture Oxyty in base is higher than edge hole, and the cultivation results for causing different holes difference are occurred by this, so as to cannot really reflect not With the cell growth status in hole, there is deviation in the selection result, may cause to have selected the algae of poor performance and miss excellent algae The consequence of kind.Therefore it is very necessary to develop a kind of microwell plate for being suitble to photosynthetic microdisk electrode.

Utility model content

It, can using the utility model the purpose of this utility model is to provide a kind of for cultivating the microwell plate of photosynthetic microalgae Effectively solve the problem of that the cultivation results in the poor different location micropore of conventional microplates gas exchange capacity are susceptible to deviation.

The overall technology of the utility model is conceived：

For cultivating the microwell plate of photosynthetic microalgae, including substrate, it is opened on substrate and in the micropore of matrix distribution, lid is set In the cover board of upper surface of base plate；Cover board and substrate are made of transparent material, and lid surface is equipped with adaptation corresponding with micropore and can The plug of inserting cooperation is realized with it, the adjacency section of micropore and plug is equipped with containment member, and plug is hollow tubular structure and bottom Portion is equipped with strainer, and the sterile packing with airing function is filled in the plug inner cavity on strainer top.

Applicant is it should be noted that micropore plate surface the number of openings and aperture specification belong to the prior art, transparent material It can easily select with preferable translucency and convenient for molding existing material, therefore applicant is to the system of cover board and substrate Make the particular contents such as material, the micropore quantity on substrate and aperture specification to repeat no more.Plug height should meet it and block shape State lower bottom part avoids the contact with culture medium in micropore, can according to the height of micropore, the application environment of culture plate, content The actual conditions such as loading amount are designed and regulate and control, all without departing from the technical essence of the utility model.

The particular technique design of the utility model also has：

For ease of plug and the quick assembling of micropore, while meet positioning action therebetween, preferred technical solution It is that the opening of the micropore is in the taper hole shape extended out.

The main function of sterile packing is the gas exchanges ensured in micropore during photosynthetic microdisk electrode with the external world, together When the culture of photosynthetic microalgae should be made to be under gnotobasis, material selection may be used a variety of existing natural or artificial synthesized Fiber Materials, wherein more preferred technical solution is, the sterile packing selects sterile cotton.

The main function of containment member is under the action of the positioning of substrate and cover board is met, and realizes micropore and external environment Isolation, to avoid gas exchanges are generated between adjacent cells, preferred technical solution is that the containment member selects sealing Circle.

For ease of realizing that the cooperation of cover board and substrate is stablized, preferred technical solution is that the adjacency section of cover board and substrate is set There is the retaining mechanism being mutually adapted.

Retaining mechanism may be used a variety of existing mechanisms and realize, all without departing from the technical essence of the utility model, wherein excellent The technology realization rate of choosing, which is that retaining mechanism uses, to be respectively arranged on the outside of substrate and in the flange that cover board surrounding extends downwardly The buckle on surface.Applicant it should be noted that flange may be used with the one-time formed structure of cover plate main body, can also be used with Cover plate main body is fixed but junction has the structure of easy folding line, and the buckle structure under above-mentioned turnup structure is all without departing from this practicality Novel technical spirit.To realize that cooperation is secured, buckle is symmetrically disposed on the mating surface of cover board and substrate periphery and in bar shaped point Cloth.

Applicant it should be noted that：

Term " outside ", " inner surface ", " upper surface ", " top ", " bottom ", " top in the description of the present invention, The orientation or position relationship of the instructions such as portion " are based on orientation shown in the drawings or position relationship, are for only for ease of simplified description The utility model rather than instruction imply that signified device or element must have specific orientation, with specific orientation structure It makes and operates, therefore it is not intended that limitation to the utility model.

Technological progress acquired by the utility model is：

1st, the utility model is using being equipped with the hollow plug that coordinates with micropore on the cover board, and passes through and set strainer and sterile Filler has been effectively ensured photosynthetic microalgae in incubation with the gas exchanges of external environment, has overcome existing microwell plate and exist Because of edge effect caused by gas circulation difference when cultivating photosynthetic microalgae, the problem of avoiding the screening deviation because of its generation.

2nd, the use of containment member while ensureing that environment is in germ-free condition in micropore, further avoids adjacent Gas exchanges between micropore.

3rd, micropore open top while plug is realized with micropore quick assembling, is convenient for using the taper hole structure extended out Meet positioning therebetween.

4th, the use of retaining mechanism, the cooperation for being easy to implement cover board and substrate are stablized.

Description of the drawings

Fig. 1 is the structure diagram of the utility model.

Fig. 2 is Fig. 1 vertical views.

Fig. 3 is the bottom view of Fig. 2.

Reference numeral in attached drawing is as follows：

1st, sterile packing；2nd, cover board；3rd, it is buckled；4th, plug；5th, sealing ring；6th, strainer；7th, substrate；8th, micropore.

Specific embodiment

The utility model is further described with reference to embodiments, but should not be construed as the limit to the utility model Fixed, the scope of protection of the utility model is subject to the content of claim record, any equivalent skill made according to specification Art means are replaced, all without departing from the scope of protection of the utility model.

The overall structure diagram of the present embodiment including substrate 7, is opened on substrate 7 and in the micropore 8 of matrix distribution, lid Cover board 2 set on 7 upper surface of substrate；Cover board 2 and substrate 7 are made of transparent material, and 2 surface of cover board is equipped with corresponding with micropore 8 It is adapted to and the plug 4 for inserting cooperation can be realized with it, the adjacency section of micropore 8 and plug 4 is equipped with containment member, and plug 4 is hollow Tubular structure and bottom are equipped with strainer 6, and the sterile packing 1 with airing function is filled in 4 inner cavity of plug on 6 top of strainer.

The opening of the micropore 8 is in the taper hole shape extended out.

The sterile packing 1 selects sterile cotton.

The containment member selects sealing ring 5.

Cover board 2 and the adjacency section of substrate 7 are equipped with the retaining mechanism being mutually adapted.

Retaining mechanism uses the card of flange inner surface for being respectively arranged at that on the outside of substrate 7 and 2 surrounding of cover board extends downwardly Button 3.

Remaining content is for example aforementioned.

Claims (6)

1. for cultivating the microwell plate of photosynthetic microalgae, including substrate (7), it is opened on substrate (7) and in the micropore of matrix distribution (8), it is covered on the cover board (2) of substrate (7) upper surface；It is characterized in that cover board (2) and substrate (7) are made of transparent material, Cover board (2) surface is equipped with the plug (4) being adapted to and inserting cooperation can be realized with it corresponding with micropore (8), micropore (8) and plug (4) adjacency section is equipped with containment member, and plug (4) is equipped with strainer (6) for hollow tubular structure and bottom, strainer (6) top Sterile packing (1) of the filling with airing function in plug (4) inner cavity.

2. the microwell plate according to claim 1 for being used to cultivate photosynthetic microalgae, it is characterised in that the top of the micropore (8) Portion's opening is in the taper hole shape extended out.

3. the microwell plate according to claim 1 for being used to cultivate photosynthetic microalgae, it is characterised in that the sterile packing (1) sterile cotton is selected.

4. the microwell plate according to claim 1 for being used to cultivate photosynthetic microalgae, it is characterised in that the containment member choosing With sealing ring (5).

5. the microwell plate for being used to cultivate photosynthetic microalgae according to any one of claim 1-4, it is characterised in that cover board (2) The retaining mechanism being mutually adapted is equipped with the adjacency section of substrate (7).

6. the microwell plate according to claim 5 for being used to cultivate photosynthetic microalgae, it is characterised in that the retaining mechanism is adopted With being respectively arranged at the buckle (3) of flange inner surface that on the outside of substrate (7) and cover board (2) surrounding extends downwardly.