CN206956034U - A kind of aerobic bacteria plant height flux screening test tube - Google Patents

A kind of aerobic bacteria plant height flux screening test tube Download PDF

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Publication number
CN206956034U
CN206956034U CN201720837826.2U CN201720837826U CN206956034U CN 206956034 U CN206956034 U CN 206956034U CN 201720837826 U CN201720837826 U CN 201720837826U CN 206956034 U CN206956034 U CN 206956034U
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China
Prior art keywords
pedestal
deep hole
lid
test tube
plant height
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Active
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CN201720837826.2U
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Chinese (zh)
Inventor
郝荣华
张晓元
刘飞
朱希强
凌沛学
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Shandong Academy of Pharmaceutical Sciences
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Shandong Academy of Pharmaceutical Sciences
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Abstract

The utility model provides a kind of aerobic bacteria plant height flux screening test tube, including the pedestal of rectangle and the lid that can be snapped on pedestal;The lid includes four sides and top;The lid opposite sides face has the card article and neck of mutual cooperation with pedestal respective sides face;There is diaphragm seal between the top of the lid and the upper surface of pedestal;The pedestal is provided with some deep holes in upper surface vertical direction;There is stirring ball inside the deep hole;Deep hole bottom is connected to by flexible or elastic connection structure;The top of the lid and the correspondence position of deep hole opening are air-vent, and vent diameter is not more than deep hole diameter;Ventilated membrane is set at air-vent.The utility model is designed using porous plate, can carry out a large amount of screening tests simultaneously;Can solve oxygen supply demand, ensure abundant dissolved oxygen;Can effectively solve the problems such as culture medium evaporation, cross pollution again, be provided for aerobic bacterial strain high flux screening and a kind of facilitate feasible easy-operating experiment equipment.

Description

A kind of aerobic bacteria plant height flux screening test tube
Technical field
The utility model belongs to experiment equipment field, and in particular to a kind of test tube for aerobic bacteria plant height flux screening, Self-carrying mixing and ventilated membrane cover body structure.
Background technology
The glass apparatus that test tube is commonly used as biology laboratory, the oscillation and fermentation cultivation of bacterium, saccharomycete etc. is generally used for, And for preserving strain, switching shaking flask expands culture or tunning Activity determination etc..Traditional test tube is necessary during bacterium for shaking Cap test tube is coordinated singly can only only to be operated using closed test tube mouth is carried out;In multiple test tubes simultaneously in use, different samples must be identified Title or numbering are in order to avoid obscure., it is necessary to several single bacterium colonies of picking during strain mutagenesis breeding screening or transformant screening Cultivated in test tube, so every test tube is individually operated, wastes time and energy.Existing multi-well microplate can also be used for strain fermentation training Support, but these microwell plate design size standards are more unified, often supported the use with automated laboratory instrument, specification is limited. When carrying out microbial fermentation using microwell plate, microwell plate can only lie against shaking table, and high speed shaken cultivation is handed between easily causing hole Fork pollution, and because lid for micro plate and plate body gap are larger, fermentation period length easily causes culture medium evaporation capacity big, so that training Support base each component concentration to change, have a strong impact on the stability of fermentation culture conditions.In addition, microwell plate liquid amount is less, far Far from meeting aerobic microbiological lengthy fermentation demand.Therefore, the high flux test tube for designing a suitable strain fermentation can be same When solve the above problems, it is significant.
The content of the invention
For at present without be adapted to bacterial strain high flux screening test tube the problem of, the utility model provide one kind can HTHP go out Bacterium, aerobic bacteria plant height flux screening test tube easy to use.
To achieve the above object, the utility model adopts the following technical scheme that.
A kind of aerobic bacteria plant height flux screening test tube, include the pedestal and a lid that can be snapped on pedestal of a rectangle Body;
The lid includes four sides and top;There is phase interworking in the lid opposite sides face with pedestal respective sides face The card article and neck of conjunction;
There is diaphragm seal between the top of the lid and the upper surface of pedestal, the diaphragm seal composition is elastomeric material, Gu Due to the top of lid or the upper surface of pedestal;
The pedestal is provided with some deep holes in upper surface vertical direction;The deep hole arranges in a manner of m row n rows, m and n It is the positive integer more than 1;Preferably, deep hole number is 3 integral multiple;
The bottom shape of the deep hole is flat, round bottom or conical bottom;
On the upside of the pedestal upper surface and there are some different identifications on right side, with deep hole each row and column position correspondence;
There is stirring ball inside the deep hole, it is internal to be hollow or solid;It is connected to by flexible or elastic connection structure Deep hole bottom;
The top of the lid and the correspondence position of deep hole opening are air-vent, and vent diameter is not more than deep hole diameter, Ventilated membrane is set at air-vent.
Above-mentioned aerobic bacteria plant height flux screening test tube is made up of high temperature high-pressure resistant material.
Aerobic bacteria plant height flux screening test tube described in the utility model when in use, passes through the top of lid and pedestal Each deep hole is divided into separate space by the sealing device between upper surface, when shaking speed is very fast, the liquid in each deep hole Body will not mutually pollute;Different samples are made a distinction by the mark on base-plates surface;Stirring ball in deep hole is due to buoyancy Or be suspended from by the support of attachment structure in liquid, because inertia internally stirs liquid when shaking table rotates, make oxygen, support Material, temperature are more uniformly distributed;Ventilated membrane on air-vent does not influence air and enters test tube while moisture evaporation is reduced.
The utility model has advantages below:
Designed using porous plate, compared with the single test tube of tradition, be easy to extensive high flux screening to work, it is simple to operate fast Victory, high pressure steam sterilization material can recycle the high flux test tube, cost-effective;Use and deep hole number set with 3 multiple, It is easy to selection of the specific experiment to consumptive material, Setup Experiments repeat, and ensure the reappearance of experiment;Made using not co-content specification high Agitating device is arranged at flux test tube, test tube bottom, and lid is ventilative, compared with conventional microplates, can meet the need of aerobic bacteria fermentation Oxygen amount, can effectively solve the problems, such as culture medium evaporation, cross pollution again.
Aerobic bacteria plant height flux screening test tube of the present utility model is designed using porous plate, can carry out a large amount of screening examinations simultaneously Test;Can solve oxygen supply demand, ensure abundant dissolved oxygen;Can effectively solve the problems such as culture medium evaporation, cross pollution again, be aerobic bacteria The offer of plant height flux screening is a kind of to facilitate feasible easy-operating experiment equipment.
Brief description of the drawings
Fig. 1 is the schematic side view of aerobic bacteria plant height flux screening test tube in embodiment 1;
Fig. 2 is the schematic top plan view of aerobic bacteria plant height flux screening test tube pedestal in embodiment 1;
Fig. 3 is that ball schematic diagram is stirred in embodiment 2;
Wherein, 1 pedestal, 2 lids, 3 sides, 4 tops, 5 card articles, 6 necks, 7 diaphragm seals, 8 deep holes, 9 stirring balls, 10 ropes, 11 air-vents, 12 ventilated membranes, 13 spring levers.
Embodiment
The utility model is described further with reference to embodiment and accompanying drawing, but not limited by following embodiments.
Embodiment 1
A kind of aerobic bacteria plant height flux screening test tube, as depicted in figs. 1 and 2, including the pedestal 1 of a rectangle and one can The lid 2 being snapped on pedestal;
The lid 2 includes four sides 3 and top 4;The opposite sides face 3 of lid 2 has with the respective sides face of pedestal 1 The card article 5 and neck 6 of mutual cooperation;
There is diaphragm seal 7 between the top 4 of the lid 2 and the upper surface of pedestal 1, the material of diaphragm seal 7 is silica gel, Gu Due to the top 4 of lid 2 or the upper surface of pedestal 1;
The upper surface vertical direction of the pedestal 1 is provided with the deep hole 8 of 96 round bottoms, the row of totally 12 row 8;
Reference numbers 1-12 on the upside of the upper surface of pedestal 1, right side mark A-H, with each row and column position correspondence of deep hole 8;
There is hollow stirring ball 9 inside the deep hole 8;The bottom of deep hole 8 is connected to by rope 10;
The correspondence position that the top 4 of the lid 2 is open with deep hole 8 is air-vent 11, and the diameter of air-vent 11 is less than deep hole 8 Diameter, ventilated membrane 12 is set at air-vent 11.
Embodiment 2
As shown in figure 3, a kind of aerobic bacteria plant height flux screening test tube, same as Example 1;Difference is, the bottom of deep hole 8 For taper;There is solid stirring ball 9 inside the deep hole 8;The bottom of deep hole 8 is connected to by spring lever 13.

Claims (7)

1. a kind of aerobic bacteria plant height flux screening test tube, it is characterised in that pedestal including rectangle and one can be snapped in Lid on pedestal;
The lid includes four sides and top;There is mutual cooperation in the lid opposite sides face with pedestal respective sides face Card article and neck;
There is diaphragm seal between the top of the lid and the upper surface of pedestal;
The pedestal is provided with some deep holes in upper surface vertical direction;There is stirring ball inside the deep hole;Pass through flexible or elasticity Attachment structure is connected to deep hole bottom;
The top of the lid and the correspondence position of deep hole opening are air-vent, and vent diameter is not more than deep hole diameter;It is ventilative Ventilated membrane is set at hole.
2. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that diaphragm seal is fixed on lid Top or the upper surface of pedestal.
3. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that the deep hole is in m row n rows Mode arranges, and m and n is the positive integer more than 1.
4. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that deep hole number is 3 integer Times.
5. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that the bottom shape of deep hole is flat Bottom, round bottom or conical bottom.
6. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that on the upside of the pedestal upper surface With m different identification, there is n different identification on right side, with the every row position correspondence of deep hole each column.
7. aerobic bacteria plant height flux screening test tube according to claim 1, it is characterised in that inside stirring ball to be hollow or It is solid.
CN201720837826.2U 2017-07-11 2017-07-11 A kind of aerobic bacteria plant height flux screening test tube Active CN206956034U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720837826.2U CN206956034U (en) 2017-07-11 2017-07-11 A kind of aerobic bacteria plant height flux screening test tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720837826.2U CN206956034U (en) 2017-07-11 2017-07-11 A kind of aerobic bacteria plant height flux screening test tube

Publications (1)

Publication Number Publication Date
CN206956034U true CN206956034U (en) 2018-02-02

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ID=61382830

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720837826.2U Active CN206956034U (en) 2017-07-11 2017-07-11 A kind of aerobic bacteria plant height flux screening test tube

Country Status (1)

Country Link
CN (1) CN206956034U (en)

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