CN209243076U - A kind of suspension cell culture device - Google Patents
A kind of suspension cell culture device Download PDFInfo
- Publication number
- CN209243076U CN209243076U CN201821661456.2U CN201821661456U CN209243076U CN 209243076 U CN209243076 U CN 209243076U CN 201821661456 U CN201821661456 U CN 201821661456U CN 209243076 U CN209243076 U CN 209243076U
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- CN
- China
- Prior art keywords
- incubator
- hole
- ontology
- support tube
- adapter
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Abstract
The utility model relates to a kind of suspension cell culture device, incubator includes ontology, gas adapter and culture solution adapter, and ontology is seal；Gas adapter and the culture solution adapter respectively pass through the ontology and are connected to the inner sealing of the ontology；The structure of gas adapter are as follows: through-hole three is provided on the wall of the incubator, sealed set has support tube one in through-hole three, which penetrates the incubator by through-hole three, and the inside of support tube one is for wearing snorkel；Alternatively, the structure of culture solution adapter are as follows: be provided with through-hole four on the wall of incubator, sealed set has support tube two in through-hole four, which penetrates the incubator by through-hole four, and the inside of support tube two is for wearing perfusion tube.The utility model structure is simple, during carrying out cell culture, without opening end cap addition culture solution and conveying air, can be operated under sealed conditions completely, so that it is guaranteed that the process entirely cultivated is gone on smoothly.
Description
Technical field
The utility model relates to equipment of chemical industry technical field, especially a kind of incubator for cultivating suspension cell.
Background technique
Culture dish is a kind of for microorganism or the labware of cell culture, tradition by a flat circle plate-like bottom
It forms with a lid, is generally made of glass or plastics.Culture dish material is essentially divided into two classes, predominantly plastic and glass
, glass can be used for vegetable material, microculture, and the adhere-wall culture of zooblast may also be used.The possibility of plastics
Polythene material, once property and it is nonexpondable, be suitble to laboratory inoculation, scribing line, the operation of separation of bacterial, can be with
Culture for vegetable material.Culture for cell needs to cultivate in the culture vessel of sealing.
For existing sealing culture vessel, cell may be will affect by conveying to the nutrient in culture vessel
Culture may pollute culture cell, and operation may be inconvenient, can not specifically learn the specific ring inside culture vessel
Border, or the environment in incubator preferably may can not be adjusted or be kept because of the sealing bad ingredient influenced in incubator, nothing
Method guarantees the accurate progress of experiment, it is therefore desirable to one kind is proposed under conditions of not influencing cell culture, effectively to incubator
The conveying of culture solution is carried out, and the gas in incubator is adjusted in time according to the environment in incubator, to guarantee
The validity of experiment.
Utility model content
The utility model is intended to provide a kind of suspension cell culture ware, to realize and suspend carefully in the environment of excellent sealing
The conveying of born of the same parents' culture solution and the adjusting of environmental gas.Further, the state-detection and display of environment are realized.
The suspension cell culture device of the utility model, the incubator include ontology, gas adapter and culture solution switching
Head, the ontology are seal, the gas adapter and the culture solution adapter respectively pass through the ontology and with it is described
The inner sealing of ontology is connected to, for conveying the suspension cell liquid in the incubator and adjusting environmental gas content.The gas
The structure of body adapter are as follows: through-hole three is provided on the wall of the incubator, sealed set has support tube one in the through-hole three,
The support tube one penetrates the incubator by the through-hole three, and the inside of the support tube one is for wearing snorkel；Alternatively,
The structure of the culture solution adapter are as follows: through-hole four is provided on the wall of the incubator, there is sealed set in the through-hole four
Support tube two, the support tube two penetrate the incubator by the through-hole four, and the inside of the support tube two is defeated for wearing
Liquid pipe.
Further, the incubator further includes temperature detection display device, the induction of the temperature detection display device
End is arranged in the body interior, and the outside of the incubator is arranged in the display end of the temperature detection display device, described
The signal that induction end will test is transmitted to the display end；Preferably, the induction end is connected by signal wire and the display end
It connects, incubator described in the signal wire hermetically passing.
Further, the temperature detection display device further includes induction end hold item, and the incubator is arranged in
Inside, for placing the induction end；Preferably, the induction end hold item is mesh basket.
Further, end face is equipped with the through-hole one for placing the induction end hold item at the upper side of the ontology,
The upper end of the induction end hold item is tightly connected by sealant and the through-hole one.
Further, the temperature detection display device further includes sealing ring, and the sealing ring seals the through-hole one；It is excellent
Selection of land, the sealing ring are equipped with the through-hole two for wearing the signal wire.
Further, the snorkel and the support tube one interference fit connect；The snorkel is equipped with closure valve,
Opening and closing for snorkel operate.
Further, the gas adapter is additionally provided with sealing ring one, for sealing the through-hole three.
Further, the perfusion tube and the support tube two interference fit connect；The perfusion tube is equipped with closure valve,
Opening and closing for perfusion tube operate.
Further, the culture solution adapter is additionally provided with sealing ring two, for sealing the through-hole four.
Seal washer is equipped between the end cap and ontology end cap mouth of the ontology, the end cap of the ontology is placed on ontology end cap
Again by sealing glue sealing after mouthful.The incubator is additionally provided with pressure sensor, and sealing is connected to the incubator and will inspection
End is surveyed to be set in the incubator.
The incubator of the culture suspension cell of the utility model, connects culture solution infusion by culture solution adapter first
Pipe injects culture solution toward incubator the inside, then connects snorkel by gas adapter, and then adjust incubator internal gas
Such as oxygen content.During cultivating suspension cell, by temperature detection display device detect incubator inside temperature and
Humidity is monitored it.It, can without opening end cap addition culture solution and conveying air during carrying out cell culture
It is operated under sealed conditions completely, so that it is guaranteed that the process entirely cultivated is gone on smoothly.The utility model overall structure
Simply, operation is easy.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model embodiment；
Fig. 2 is the partial enlarged view in Fig. 1 at A；
Fig. 3 is the partial enlarged view in Fig. 1 at B；
Fig. 4 is the partial enlarged view in Fig. 1 at C；
Drawing reference numeral explanation:
1. ontology；2. ontology end cap；3. temperature detection display device；4. gas adapter；5. culture solution adapter；6. rubber
Glue supporting block；31. display；32. induction end；33. mesh basket；34. through-hole one；35. sealing ring；41. snorkel；42. closure valve
One；43. support tube one；44. through-hole three；45. sealing ring one；46. through-hole five；51. perfusion tube；52. closure valve two；53. support
Pipe two；54. through-hole four；55. sealing ring two；56. through-hole six.
Specific embodiment
The utility model is understood and realized for the ease of persons skilled in the art, and it is practical new to describe this now in conjunction with attached drawing
The embodiment of type.
The utility model proposes a kind of structures simply, can realize suspension cell culture liquid in the environment of excellent sealing
The suspension cell culture device of the adjusting of transport environment gas.
Specifically, the suspension cell culture device of the utility model, the incubator mainly include ontology, gas adapter and
Culture solution adapter.The ontology is seal, can be sealed traditional container with incubator lid near lid.
Seal washer can be equipped between the end cap and ontology end cap mouth of the ontology, the end cap of the ontology is placed on after ontology end cap mouth again
It can be by sealing glue sealing.Certainly vessel not with cover also may be selected.
The incubator further includes temperature detection display device, and temperature detection display device sealed set is on the body
Side is detected and is shown with humidity parameter for the temperature to culture cell.Temperature detection display device is preferably provided at training
Support a upper side of device.Gas adapter and culture solution adapter respectively pass through the ontology and the inner sealing with the ontology
Connection, for conveying the suspension cell liquid in the incubator and adjusting environmental gas content.Gas adapter and culture solution turn
Connector is preferably provided on the side wall of incubator.
The structure of temperature detection display device may include induction end and display end.Induction end is arranged in the body interior,
It specifically may be provided at the top of culture solution.The outside of the incubator is arranged in the display end of temperature detection display device, induction
The signal that will test is held to be transmitted to the display end.Induction end and the signal communication of display end can be wireless transmissions, or be signal
Line transmission.Preferably, the induction end is connect by signal wire with the display end, is cultivated described in the signal wire hermetically passing
Device.
Further, temperature detection display device may also include induction end hold item, be arranged in the incubator
Portion, for placing the induction end.The induction end hold item can be shelf class formation or reticular structure.Preferably, the induction
End hold item is mesh basket.
Further, in order to which hold item is fixed, end face is equipped with and puts for placing induction end at the upper side of ontology
The through-hole one of part is set, the upper end of induction end hold item can be tightly connected by sealant and the through-hole one.Temperature detection is shown
Device may also include sealing ring, and sealing ring seals through-hole one.Preferably, sealing ring is equipped with for wearing the logical of the signal wire
Hole two.
The structure of gas adapter may be designed as: through-hole three is provided on the wall of incubator, there is sealed set in through-hole three
Support tube one, the support tube one penetrate the incubator by through-hole three, and the inside of support tube one is for wearing snorkel.
Specifically, snorkel can be interference fitted with support tube one and connect.For the ease of the on-off of gas, snorkel is equipped with
Closure valve, the opening and closing for snorkel operate.
Further, the gas adapter can also be equipped with sealing ring one, for sealing through-hole three.
The structure of culture solution adapter may be designed as: through-hole four, sealed set in through-hole four are provided on the wall of incubator
There is support tube two, which penetrates the incubator by through-hole four, and the inside of support tube two is for wearing perfusion tube.
Specifically, perfusion tube can be interference fitted with support tube two and connect.For the ease of the on-off of culture solution, set on perfusion tube
There is closure valve, the opening and closing for perfusion tube operate.
Further, culture solution adapter can also be equipped with sealing ring two, for sealing through-hole four.
In order to understand the pressure condition in culture dish in real time, the incubator is also provided with pressure sensor, sealing
It is connected to the incubator and will test end and be set in the incubator.
When to incubator operation, culture solution perfusion tube is connected by culture solution adapter first, toward the injection of incubator the inside
Then culture solution connects snorkel by gas adapter, and then adjusts the content of incubator internal gas such as oxygen.It is cultivating
During suspension cell, temperature and the pressure etc. inside incubator can be detected by temperature detection and pressure detecting display device
Parameter is monitored it, so as to which preferably the environment in incubator is adjusted.In the process for carrying out cell culture
In, without opening end cap addition culture solution and conveying air, can be operated under sealed conditions completely, so that it is guaranteed that entirely
The process of culture is gone on smoothly.The utility model overall structure is simple, and operation is easy.
Embodiment
If Fig. 1 is the structural schematic diagram of the utility model, a kind of culture vessel for cultivating suspension cell, including ontology 1, this
1 bottom of body is equipped with rubber block 6, is equipped with ontology end cap 2 above ontology 1.It is aobvious equipped with temperature detection at the upper side of the ontology 1
Showing device 3, the temperature detection display device 3 include display 31, and the display 31 is connected with induction end by signal wire
32, the mesh basket 33 for placing induction end 32 is equipped at the upper side of the ontology 1,1 side of ontology is equipped with gas adapter 4
With culture solution adapter 5, the gas adapter 4 connects snorkel 41, and the snorkel 41 is equipped with closure valve 1, described
1 side of ontology is equipped with the support tube 1 for wearing snorkel 41.The culture solution adapter 5 connects perfusion tube 51, described
Perfusion tube 51 is equipped with support tube 2 53 equipped with closure valve 2 52,1 side of ontology and positioned at one 43 lower section of support tube.Ontology
End cap between ontology end cap mouth be equipped with seal washer, after ontology end cap 2 is placed on ontology end cap mouth again with sealing glue into
Row sealing guarantees the whole leakproofness of ontology 1.Since ontology 1 is glassware in itself, being sealed with sealing glue can reach
To the effect of sealing, at the same time, even if being also that can be cut by sharp stage property after the dry solidification of glue on glass
Removal, so not interfering the disassembly after experiment, it will not influence the quality of ontology 1 itself.
Referring to figs. 1 and 2,1 upper side end face of ontology is equipped with for placing the through-hole 1 for wearing mesh basket 33.
33 port of mesh basket is adhesively fixed by sealing glue in 1 inside of ontology.One 34 upper end of through-hole has by sealant hydropexis
Sealing ring 35, the sealing ring 35 are equipped with the through-hole 2 36 for wearing signal wire.Induction end 32 is being mounted on mesh basket 33
Afterwards, signal wire pass through through-hole 2 36 reconnect on the display 31, this be engraved at through-hole 2 36 with seal glue it is carried out it is close
Envelope, and then ensure that the leakproofness of entire ontology 1.
In conjunction with shown in Fig. 3 and Fig. 4,1 side of ontology, which is equipped with, is respectively used to the logical of fixed support tube 1 and support tube 2 53
One end of hole 3 44 and through-hole 4 54, the support tube 1 and support tube 2 53 passes through sealant hydropexis in through-hole three respectively
44 and through-hole 4 54 on and the other end towards the inside of ontology 1.Through-hole 3 44 and 4 54 aperture of through-hole pass through sealing glue respectively
It is fixed with sealing ring 1 and sealing ring 2 55.The sealing ring 1 is equipped with the through-hole 5 46 for wearing snorkel 41,
The sealing ring 2 55 is equipped with the through-hole 6 56 for wearing perfusion tube 51.Distinguished by closure valve 1 and closure valve 2 52
Snorkel 41 and perfusion tube 51 are opened and closed, the circulation of air-flow inside and outside ontology 1 is avoided.Snorkel 41 connects air pump at this time
Air-flow in its ontology 1 is exported and inputted, perfusion tube connects culture solution cabinet, and nutrition is added in timing in ontology 1
Liquid.
In conjunction with shown in Fig. 3 and Fig. 4, snorkel 41 and perfusion tube 51 are matched with support tube 1 and 2 53 interference of support tube respectively
Connection is closed, with sealant water process at the connector of snorkel 41 and support tube 1, in perfusion tube 51 and support tube 2 53
Connector at sealant water process, to guarantee the leakproofness of entire vessel.
In use, first by culture solution adapter 5 connect culture solution perfusion tube 51, toward ontology 1 inside inject culture solution,
Then snorkel 41 is connected by gas adapter 4, and then adjusts the oxygen content of body interior, in the mistake of culture suspension cell
Cheng Zhong is monitored it by the temperature inside temperature detection display device detection body.In the process for carrying out cell culture
In, culture solution and conveying air are added without opening ontology end cap 2, can be operated under sealed conditions completely, thus really
The process entirely cultivated is protected to go on smoothly.
Found out by above-described embodiment, the utility model overall structure is simple, and operation is easy, and is being fully sealed with can be convenient
In the case where incubator is operated.
Although depicting the utility model by embodiment, it will be appreciated by the skilled addressee that not departing from this reality
In the case where with novel spirit and essence, so that it may make the utility model there are many deformation and variation, it all should be in the utility model
Protection scope in.
Claims (13)
1. a kind of cell culture apparatus, which is characterized in that the incubator includes ontology, gas adapter and culture solution adapter,
The ontology is seal, wherein
The gas adapter and the culture solution adapter respectively pass through the ontology and connect with the inner sealing of the ontology
It is logical；
The structure of the gas adapter are as follows: through-hole three, sealed set in the through-hole three are provided on the wall of the incubator
There is support tube one, which penetrates the incubator by the through-hole three, and the inside of the support tube one is for wearing
Snorkel；Or
The structure of the culture solution adapter are as follows:
Through-hole four is provided on the wall of the incubator, sealed set has support tube two in the through-hole four, the support tube two-way
It crosses the through-hole four and penetrates the incubator, the inside of the support tube two is for wearing perfusion tube.
2. incubator according to claim 1, which is characterized in that the incubator further includes temperature detection display device,
The induction end of the temperature detection display device is arranged in the body interior, and the display end of the temperature detection display device is set
It sets in the outside of the incubator, the signal that the induction end will test is transmitted to the display end.
3. incubator according to claim 2, which is characterized in that the induction end is connected by signal wire and the display end
It connects, incubator described in the signal wire hermetically passing.
4. incubator according to claim 2, which is characterized in that the temperature detection display device further includes that induction end is put
Part is set, the inside of the incubator is set, for placing the induction end.
5. incubator according to claim 4, which is characterized in that the induction end hold item is mesh basket.
6. incubator according to claim 4, which is characterized in that end face is equipped with for placing at the upper side of the ontology
The upper end of the through-hole one of the induction end hold item, the induction end hold item is connected by sealant and the through-hole one sealing
It connects.
7. incubator according to claim 4, which is characterized in that the temperature detection display device further includes sealing ring,
The sealing ring seals the through-hole one.
8. incubator according to claim 3, which is characterized in that the temperature detection display device further includes that induction end is put
Part is set, the inside of the incubator is set, for placing the induction end；The temperature detection display device further includes close
Seal, the sealing ring seal the through-hole one；The sealing ring is equipped with the through-hole two for wearing the signal wire.
9. incubator according to claim 1, which is characterized in that the snorkel and the support tube one interference fit connect
It connects；The snorkel is equipped with closure valve one.
10. incubator according to claim 1, which is characterized in that the gas adapter is additionally provided with sealing ring one, is used for
Seal the through-hole three.
11. incubator according to claim 1, which is characterized in that set between the end cap and ontology end cap mouth of the ontology
There is seal washer, the end cap of the ontology is placed on after ontology end cap mouth again by sealing glue sealing.
12. incubator according to claim 1, which is characterized in that the perfusion tube and the support tube two are interference fitted
Connection；The perfusion tube is equipped with closure valve two.
13. incubator according to claim 1, which is characterized in that the culture solution adapter is additionally provided with sealing ring two, uses
In the sealing through-hole four；The incubator is additionally provided with pressure sensor, and sealing is connected to the incubator and will test end
It is set in the incubator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821661456.2U CN209243076U (en) | 2018-10-12 | 2018-10-12 | A kind of suspension cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821661456.2U CN209243076U (en) | 2018-10-12 | 2018-10-12 | A kind of suspension cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209243076U true CN209243076U (en) | 2019-08-13 |
Family
ID=67524830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821661456.2U Expired - Fee Related CN209243076U (en) | 2018-10-12 | 2018-10-12 | A kind of suspension cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209243076U (en) |
-
2018
- 2018-10-12 CN CN201821661456.2U patent/CN209243076U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190813 Termination date: 20201012 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |