CN202942889U

CN202942889U - Sample-adding box for multi-channel pipette

Info

Publication number: CN202942889U
Application number: CN201220538992XU
Authority: CN
Inventors: 李军; 韩亚萍; 刘婷; 党旖旎; 蒋龙凤; 刘源; 张冬月; 杨希; 洪峰
Original assignee: Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Current assignee: Jiangsu Province Hospital First Affiliated Hospital With Nanjing Medical University
Priority date: 2012-10-17
Filing date: 2012-10-17
Publication date: 2013-05-22
Anticipated expiration: 2022-10-17

Abstract

The utility model relates to a sample-adding box for a multi-channel pipette. The box body of the sample-adding box disclosed by the utility model comprises a base plate, a left lateral plate, a right lateral plate, a front lateral plate and a rear lateral plate, wherein a box cover is covered on the box body; scale lines are vertically arranged on the external surface of the box body; and the base plate is tilted. The sample-adding box of the multi-channel pipette disclosed by the utility model solves the problems that the operation processes are added and the opportunity of experimental pollutions is also increased because liquid can be sucked only by removing 4-10 gun heads when last few milliliters of liquid is remained in the box in the prior art. The sample-adding box of the multi-channel pipette disclosed by the utility model is simple in structure and convenient to use; the bottom of the box body is slightly tilted to one side, and an inside box included angle being 10-15 degrees is formed between the bottom of the box body and the wall of the box body at the same side, and is good for the liquid in the box to gather in the included angle; the sample-adding box can be used for cell culture, and in particular used for temporarily preparing and storing a cell suspension or a culture medium while preparing a monoclonal antibody; the liquid extraction of the multi-channel pipetting gun is facilitated; the uniform distribution of the liquid in various culture holes can be guaranteed; and the working hours can be saved.

Description

Multiple tracks liquid-transfering gun application of sample box

Technical field

The utility model belongs to experimental instrument and equipment field, particularly multiple tracks liquid-transfering gun application of sample box.

Background technology

The multiple tracks liquid-transfering gun is commonly called as the volley of rifle fire, is mainly used in the application of sample of 96 porocyte culture plates or 96 hole ELISA Plates, and the advantage of multiple tracks liquid-transfering gun is to guarantee that the liquid volume added in each hole reaches unanimously, can reduce labor intensity of operating staff again.

Before the present utility model, cultivate particularly in the experiments such as monoclonal antibody preparation carrying out cell, the experimenter can select ready-made 90mm or the circular culture dish of 150mm as prepare and deposit the utensil of culture medium temporarily according to the road number of multiple tracks liquid-transfering gun.Because culture dish is circular, highly be generally 15-20mm, when when preparing more than or equal to 40 milliliters of liquid total amounts, can't carry out mixing to culture medium in the culture dish of 90mm, and 150mm is excessive due to diameter, can't be affectedly bashful from its side and carry out mixing, add a cover rear mixing if adopt, easily make again the culture medium in culture dish unrestrained from the finger offside when fetching culture dish; Moreover, when the multiple tracks liquid-transfering gun is got liquid usually in the middle part of culture dish, padded culture dish one side bottom of minimizing needs along with liquid, liquid is focused near operator's one side, can only remove 4-10 rifle head to last several milliliters the time and just can be drawn onto liquid, so not only increase the chance that operation sequence has also increased experimental pollution.

Summary of the invention

The purpose of this utility model just is to overcome defects, development multiple tracks liquid-transfering gun application of sample box.

The technical solution of the utility model is:

Multiple tracks liquid-transfering gun application of sample box, box body comprises base plate, left and right sides plate and front and back biside plate, covers lid on it, and cartridge outer surface vertically arranges graduation mark, and its technical characteristics is that base plate is skewed.

Advantage of the present utility model and effect are reasonable in design compared with prior art, simple in structure, easy to use, use transparent polypropylene, polystyrene or glass to make a multiple tracks liquid-transfering gun application of sample box, slightly be with inclination to a side bottom this box body, with angle in the box that forms the 10-15 degree with lateral box wall, be conducive to the interior liquid of box and assemble in angle; All there is accurate graduation mark sign on box body outer wall positive (being front side board) and both sides, side (being left and right sides plate), can be used for cell cultivates the interim preparation of cell suspension when particularly monoclonal antibody prepares or culture medium and deposits, facilitate the multiple tracks liquid-transfering gun to get liquid, can guarantee that in each culture hole, uniform liquid distributes, can save man-hour again.

Description of drawings

Fig. 1---the utility model stereochemical structure principle schematic.

In figure, each label represents that parts are as follows:

Lid 1, box body 2, graduation mark 3, gripper shoe 4.

The specific embodiment

As shown in Figure 1:

Box body 2 comprises base plate, left plate, right plate, front side board, back side panel, covers 1 six face closure of lid; Many places arrange vertical graduation mark 3 on box body 2 left plates, right plate, front side board; Box body 2 is normally rectangular, and base plate tilts on the box body width, in figure, the front side board of box body 2 and the distance between back side panel are the width of box body 2, be base plate from the bottom of front side board backward and be inclined upward to the middle-end of back side panel, base plate formed angle of inclination with horizontal plane is take the 10-15 degree as good; The junction of base plate and back side panel middle-end arranges a gripper shoe 4, the bottom that gripper shoe 4 is downward and the bottom of front side board are on same horizontal plane, be exactly perhaps the back side panel that keeps complete, it is gripper shoe 4 that the junction of base plate and back side panel middle-end is used as down to the bottom.Certainly, base plate can be also that the length at box body 2 tilts, namely base plate from the left plate bottom to the right and upwards to the middle-end of right plate, remaining with aforementioned.

The utility model application note:

during use, get sterilization (through gamma-rays, E-beam ray or autoclaved) multiple tracks liquid-transfering gun application of sample box, lid 1 is moved on Biohazard Safety Equipment or superclean bench, respectively with culture medium, hyclone, cell or other experiment reagent add in box body, and the graduation mark 3 on observation box body 2 sides (being left and right sides plate) or box body 2 fronts (being front side board), the experimenter pinches box body 2 both sides with thumb and forefinger, the interior liquid of mixing box body 2 gently, and be that the low side that tilts of base plate is near the experimenter with box body 2 fronts, dividing with the multiple tracks liquid-transfering gun imbitition that installs the rifle head installs in corresponding 96 well culture plates or in 96 hole ELISA Plates, in case of necessity, before getting another piece culture plate, lid 1 can overdoed bonnet on box body 2 on alcolhol burner, reduce the air-flow generation of liquid top as far as possible, reduce the probability of experimental pollution.

Material of the present utility model comprises that box body 2, lid 1, gripper shoe 4 are good, nontoxic and relatively firm polypropylene, polystyrene or the glass of transparency, the length of box body 2 is 80-115mm, width is 70-80mm, be highly 25-35mm, inclination slightly is with to a side in box body 2 bottoms, with angle in the box that forms the 10-15 degree with lateral box wall, its objective is to allow the interior liquid of box body 2 flow to all the time angle in box, even quantity of fluid when box body 2 is interior, still can use multiple tracks volley of rifle fire application of sample to guarantee to only have seldom; Box body 2 back sides are that back side panel has the high gripper shoe of 10-15mm 4, and purpose is to keep box body 2 horizontal; Box body 2 fronts are the graduation mark 3 that front side board and side (being left and right sides plate) all indicate 10-100ml; The length of lid 1 and width each greater than the about 2-3mm of box body 2, the height of lid 1 is 6-8mm, the interior heap(ed) capacity of box body 2 is 60-100ml, the length of its box body 2 is 80-115mm, purpose is coupling 8 roads or 12 multiple tracks liquid-transfering guns, and width is 70-80mm, facilitates experimental implementation.

Due to the existence that gripper shoe 4 is arranged, whole box body 2 still can be placed on the table reposefully.

In the utility model, do not design the outer of box body 2, purpose is to prevent the interior liquid spill of box body 2 counter flowing in box body 2 again behind the surface of box body 2 outers, and this box body 2 width are less, take conveniently, do not need to design in addition handle, are beneficial to transportation and store.

The utility model can be widely used in the multiple related experiment of the industries such as biologic medical, animals and plants, particularly the liquid storage during multiple tracks liquid-transfering gun application of sample when cell is cultivated.

Claims

1. multiple tracks liquid-transfering gun application of sample box, box body comprises base plate, left plate, right plate, front side board, back side panel, covers lid on it, cartridge outer surface vertically arranges graduation mark, it is characterized in that base plate is skewed.

2. multiple tracks liquid-transfering gun application of sample box according to claim 1, is characterized in that being inclined upward to high-end side plate bottom connection gripper shoe at base plate from low side.

3. multiple tracks liquid-transfering gun application of sample box according to claim 2, is characterized in that being on same horizontal plane bottom the side plate of gripper shoe bottom and opposite side.

4. multiple tracks liquid-transfering gun application of sample box according to claim 1, is characterized in that base plate is to tilt at the box body width.

5. multiple tracks liquid-transfering gun application of sample box according to claim 1, is characterized in that being inclined upward to from low side at base plate high-end angle in the 10-15 degree.

6. multiple tracks liquid-transfering gun application of sample box according to claim 1, is characterized in that box body, lid and gripper shoe adopt polypropylene or polystyrene or glass to make.