CN114525198B - Microalgae non-contact co-culture device - Google Patents

Microalgae non-contact co-culture device Download PDF

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CN114525198B
CN114525198B CN202210070803.9A CN202210070803A CN114525198B CN 114525198 B CN114525198 B CN 114525198B CN 202210070803 A CN202210070803 A CN 202210070803A CN 114525198 B CN114525198 B CN 114525198B
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connector
magnet
culture
flange
contact
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CN114525198A (en
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姬南京
徐名扬
陈磊
王君玥
黄金旺
申欣
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Jiangsu Ocean University
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Jiangsu Ocean University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management

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Abstract

The invention discloses a microalgae non-contact co-culture device which comprises a culture bottle A and a culture bottle B, wherein the bottoms of the culture bottle A and the culture bottle B are connected with a supporting rod, the bottom of the supporting rod is connected with a chassis, one end of the culture bottle A is connected with a connector A, the outer side of the connector A is connected with a flange A, one end of the culture bottle B is connected with a connector B, the outer side of the connector B is connected with a flange B, filter membrane placing grooves are formed in the connectors A and B, magnet fixing grooves are formed in the outer sides of the filter membrane placing grooves, magnets A are installed on the magnet fixing grooves of the connector A, and magnets B are installed on the magnet fixing grooves of the connector B. This device can realize phytoplankton non-contact coculture, and the ring adsorption structure of formula is inhaled to the sampling magnetism, and the experimental procedure of changing the filter membrane that has significantly reduced, in addition, the device can purchase the different aperture filter membranes of commercialization, and the flexibility is strong, can be used to study the allelochemically effect between different phytoplanktons.

Description

Microalgae non-contact co-culture device
Technical Field
The invention relates to the field of phytoplankton non-contact co-culture, in particular to a microalgae non-contact co-culture device.
Background
Marine phytoplankton not only forms the basis of the marine food chain, but also plays an important role in the biogeochemical cycle, such as the global carbon cycle. In recent years, with the global warming, the eutrophication of water body and other problems, the community structure of phytoplankton gradually tends to be unstable, even the outbreak scale and frequency of harmful algal blooms tend to increase year by year, which seriously affects aquaculture, tourism, marine ecosystem and even endangers human health. When red tide occurs, part of red tide organisms can release allelochemicals to the environment through allelochemicals so as to inhibit or even kill the surrounding competitive organisms, thereby ensuring the competitive advantage of the red tide organisms. At present, the allelopathy and the mechanism research of the allelopathy between phytoplankton mostly adopt the method of separately culturing a research object by using a diaphragm or a dialysis bag, and the influence on the physiological characteristics of a target algae strain is compared by non-contact coculture.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a microalgae non-contact co-culture device to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a little algae non-contact co-culture device, includes blake bottle A and blake bottle B, the bottom of blake bottle A and blake bottle B is connected with the bracing piece, the bottom of bracing piece is connected with the chassis, blake bottle A's one end is connected with connector A, connector A's the outside is connected with ring flange A, blake bottle B's one end is connected with connector B, connector B's the outside is connected with ring flange B, connector A and connector B's inside is equipped with the filter membrane standing groove, the outside of filter membrane standing groove is equipped with the magnet fixed slot, install magnet A on connector A's the magnet fixed slot, install magnet B on connector B's the magnet fixed slot, ventilative membrane of sealing is installed at blake bottle A and blake bottle B's top.
As a preferable technical scheme of the invention, the bottle openings at the tops of the culture bottles A and B are conical.
As a preferred technical scheme of the invention, a plurality of fixing holes are uniformly arranged on the flange A and the flange B.
As a preferred technical solution of the present invention, the magnet a is adhered to the magnet fixing groove of the connector a by a sealant, and the magnet B is adhered to the magnet fixing groove of the connector B by a sealant.
In a preferred embodiment of the present invention, the surface of the flange a and the surface of the flange B are bonded with an annular gasket.
As a preferred technical scheme of the invention, the culture bottle A and the culture bottle B are both made of quartz glass, and the connector A, the flange B, the support rod, the chassis and the connector B are all made of transparent PVC materials through mould casting.
As a preferred technical solution of the present invention, the connector a and the connector B are fixedly connected by a magnet a and a magnet B.
Has the advantages that: this device can realize that phytoplankton non-contact cultivates altogether, and the ring adsorption structure of formula is inhaled to the sampling magnetism, and the experimental procedure of changing the filter membrane that has significantly reduced, in addition, the device can purchase the different aperture filter membranes of commercialization, and the flexibility is strong, can be used to research the allelochemical effect between different phytoplankton.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
In the figure, a culture bottle A1, a culture bottle B2, a connector A3, a flange A4, a magnet fixing groove 5, a filter membrane placing groove 6, an annular sealing gasket 7, a fixing hole 8, a flange B9, a magnet B10, a breathable sealing membrane 11, a support rod 12, a base plate 13, a connector B14 and a magnet A15.
Detailed Description
The following detailed description of the preferred embodiments of the present invention, taken in conjunction with the accompanying drawings, will make the advantages and features of the invention more readily understood by those skilled in the art, and thus will more clearly and distinctly define the scope of the invention.
As shown in fig. 1, the present invention provides a technical solution: the utility model provides a little algae non-contact coculture device, including blake bottle A1 and blake bottle B2, the bottom of blake bottle A1 and blake bottle B2 is connected with bracing piece 12, the bottom of bracing piece 12 is connected with chassis 13, blake bottle A1's one end is connected with connector A3, connector A3's the outside is connected with ring flange A4, blake bottle B2's one end is connected with connector B14, connector B14's the outside is connected with ring flange B9, connector A3 and connector B14's inside is equipped with filter membrane standing groove 6, filter membrane standing groove 6's the outside is equipped with magnet fixed slot 5, install magnet A15 on connector A3's the magnet fixed slot 5, install magnet B10 on connector B14's the magnet fixed slot 5, ventilative sealing membrane 11 is installed at blake bottle A1 and blake bottle B2's top.
The bottle openings at the tops of the culture bottles A1 and B2 are conical.
The flange A4 and the flange B9 are evenly provided with a plurality of fixing holes 8.
Magnet a15 is bonded to magnet fixing groove 5 of connector A3 by a sealant, and magnet B10 is bonded to magnet fixing groove 5 of connector B14 by a sealant.
And the surfaces of the flange A4 and the flange B9 are adhered with an annular sealing gasket 7.
The culture bottle A1 and the culture bottle B2 are made of quartz glass, and the connector A3, the flange A4, the flange B9, the support rod 12, the chassis 13 and the connector B14 are made of transparent PVC materials through mold casting.
The connector A3 and the connector B14 are fixedly connected through a magnet A15 and a magnet B10.
The working principle is as follows: the utility model provides a little algae non-contact is cultivateed device altogether, including blake bottle A1, blake bottle B2, connector A3, ring flange A4, magnet fixed slot 5, filter membrane standing groove 6, sealed pad 7, the fixed orifices 8, ring flange B9, magnet B10, ventilative sealing membrane 11, bracing piece 12, chassis 13, connector B14 and magnet A15, when using, through putting into filter membrane standing groove 6 in connector A3 and connector B14 with the filter membrane, then lean on connector A3 and connector B14 each other together, through magnet A15 and magnet B10 mutual magnetism, thereby let connector A3 and connector B14 fix together fast, and the surface of ring flange A4 and flange B9 is the annular sealed pad, improve sealing performance, the phenomenon of preventing to leak, when still there is the phenomenon of leaking, can also consolidate through ring flange A4 and flange B9, dismantle very simple and convenient, the filter membrane is convenient to change, the flexibility is strong, adopt bracing piece 12 and chassis 13 to support blake bottle A1 and blake bottle B2, the filter membrane standing groove can place the flexibility of different apertures, people's operation is strong.
This device can realize that phytoplankton non-contact cultivates altogether, and the ring adsorption structure of formula is inhaled to the sampling magnetism, and the experimental procedure of changing the filter membrane that has significantly reduced, in addition, the device can purchase the different aperture filter membranes of commercialization, and the flexibility is strong, can be used to research the allelochemical effect between different phytoplankton.
The above examples only show some embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention.

Claims (7)

1. A microalgae non-contact co-culture device comprises a culture bottle A (1) and a culture bottle B (2), and is characterized in that: the bottom of blake bottle A (1) and blake bottle B (2) is connected with bracing piece (12), the bottom of bracing piece (12) is connected with chassis (13), the one end of blake bottle A (1) is connected with connector A (3), the outside of connector A (3) is connected with ring flange A (4), the one end of blake bottle B (2) is connected with connector B (14), the outside of connector B (14) is connected with ring flange B (9), the inside of connector A (3) and connector B (14) is equipped with filter membrane standing groove (6), the outside of filter membrane standing groove (6) is equipped with magnet fixed slot (5), install magnet A (15) on magnet fixed slot (5) of connector A (3), install magnet B (10) on magnet fixed slot (5) of connector B (14), ventilative membrane (11) of sealing is installed at the top of blake bottle A (1) and blake bottle B (2).
2. The microalgae non-contact co-culture device of claim 1, wherein: the bottle openings at the tops of the culture bottles A (1) and B (2) are conical.
3. The microalgae non-contact co-culture device of claim 1, wherein: and the flange A (4) and the flange B (9) are uniformly provided with a plurality of fixing holes (8).
4. The microalgae non-contact co-culture device of claim 1, wherein: magnet A (15) bond in magnet fixed slot (5) on connector A (3) through sealed glue, magnet B (10) bond in magnet fixed slot (5) on connector B (14) through sealed glue.
5. The microalgae non-contact co-culture device of claim 1, wherein: and an annular sealing gasket (7) is adhered to the surfaces of the flange A (4) and the flange B (9).
6. The device for microalgae non-contact co-culture according to claim 1, wherein: the culture bottle A (1) and the culture bottle B (2) are made of quartz glass, and the connector A (3), the flange plate A (4), the flange plate B (9), the supporting rod (12), the chassis (13) and the connector B (14) are made of transparent PVC materials through mold pouring.
7. The device for microalgae non-contact co-culture according to claim 1, wherein: and the connector A (3) and the connector B (14) are fixedly connected through a magnet A (15) and a magnet B (10).
CN202210070803.9A 2022-01-21 2022-01-21 Microalgae non-contact co-culture device Active CN114525198B (en)

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CN114525198B true CN114525198B (en) 2022-12-06

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202849409U (en) * 2012-10-23 2013-04-03 杭州赫贝科技有限公司 Cell co-culture bottle
CN204342808U (en) * 2015-01-06 2015-05-20 郑海波 Cell culture apparatus
CN107460117A (en) * 2017-09-04 2017-12-12 清华大学 A kind of experimental provision and method for resistant gene Horizontal Gene Transfer research
WO2021038038A1 (en) * 2019-08-30 2021-03-04 Deutsches Krebsforschungszentrum Multi-cell culture device and kit
CN212955180U (en) * 2020-04-20 2021-04-13 财团法人食品工业发展研究所 Co-culture device
CN214902854U (en) * 2021-05-24 2021-11-30 中国农业大学 Novel culture device for biological interaction

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN202849409U (en) * 2012-10-23 2013-04-03 杭州赫贝科技有限公司 Cell co-culture bottle
CN204342808U (en) * 2015-01-06 2015-05-20 郑海波 Cell culture apparatus
CN107460117A (en) * 2017-09-04 2017-12-12 清华大学 A kind of experimental provision and method for resistant gene Horizontal Gene Transfer research
WO2021038038A1 (en) * 2019-08-30 2021-03-04 Deutsches Krebsforschungszentrum Multi-cell culture device and kit
CN212955180U (en) * 2020-04-20 2021-04-13 财团法人食品工业发展研究所 Co-culture device
CN214902854U (en) * 2021-05-24 2021-11-30 中国农业大学 Novel culture device for biological interaction

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