CN214902854U - Novel culture device for biological interaction - Google Patents

Abstract

The utility model relates to a biological cultivation technical field provides a novel culture apparatus for biological interaction, include: the sensing signal substance transmission device comprises a plurality of bottle bodies, wherein the bottle bodies are provided with communicating pipelines, two adjacent bottle bodies are mutually communicated through the communicating pipelines in a sleeved mode, and an isolating layer is arranged between the two mutually communicated bottle bodies, so that two organisms or two (groups) of the same organism are not in direct contact with each other, and the sensing signal substance is allowed to be fully transmitted and communicated. And the cover bodies are respectively covered on the bottle mouths of the bottle bodies in a one-to-one correspondence manner. According to the utility model discloses a novel culture apparatus for biological interaction, its seal is fabulous, can prevent effectively that sample seepage and impurity miscellaneous fungus from getting into, has avoided loaded down with trivial details complicated intercommunication operation among the prior art simultaneously, and the intercommunication effect is better, has still shortened the transmission distance of the chemical sensing signal material between different bottles, can be applied to aquatic organism and plant roots's interaction experiment, and application scope is wider, the usage is more.

Description

Novel culture device for biological interaction

Technical Field

The utility model relates to a biological cultivation technical field especially relates to a novel culture apparatus for biological interaction.

Background

In natural water, special intercalant microenvironment exists around the algal bloom cells, wherein unique microbial communities including bacteria, fungi, protozoa and other algae exist, and have intricate and complex interaction relationships (such as mutualism, paragenetic symbiosis or competitive inhibition) among them, which together constitute an important regulator of the structure and function of the water ecosystem. The interaction of algae-algae and algae-bacteria in the natural water ecosystem influences the growth and elimination dynamics of blue algae bloom and marine red tide, and has important function potential in water quality regulation and restoration. Therefore, the interaction between organisms in the interstellar microenvironment is always a hot spot for the dynamic and mechanism research of the growth and the elimination of the blue algae bloom and the ocean red tide at home and abroad.

In the prior art, when the interaction of algae-algae and algae-bacteria is researched, a mixed co-culture mode is mostly adopted, different algae species and strains are fully contacted, but different algae and bacteria colony cells in a culture medium cannot be accurately and respectively sampled, so that the cells of two mutual crop species cannot be respectively counted, the measurement of respective cell physiological indexes is also difficult, and the analysis and statistics of interaction experimental results are inconvenient. Therefore, the method of using the non-direct contact of the cells of the two groups of algae-algae and algae-bacteria to generate chemosensory signal substances (mostly secondary metabolites) capable of freely transmitting and exchanging is gradually the main means of interaction research.

The technical scheme that two algae or bacteria grow under the same environment by adopting a two-bottle communication mode is disclosed in the prior art, but the length of a communication pipe of a bottle body used by the technical scheme is longer, the caliber of the communication pipe is too narrow, a communication mechanism is complex and tedious (more fine and broken parts) and inconvenient to operate, the bottle body is not provided with a special sampling outlet, the caliber of an upper port of the bottle is narrow, a special and conveniently-taken antibacterial ventilation cover body is not arranged, the problems that the contact area of two interaction systems is small, the transfer distance of allelochemicals is long, the communication position enables the communication operation to be complex, the tightness after communication is poor, the upper port of the bottle is narrow and does not have an adaptive cover body and the like are solved, and the accuracy of an interaction experiment result is directly influenced.

In addition, the rhizosphere is an important area for information communication among different or same plant individuals, root exudates are a key factor for maintaining the vitality of a rhizosphere micro-ecosystem and are also important components of rhizosphere material circulation, and interaction mediated by root system allelopathic signal materials is generally concerned by scholars at home and abroad. However, in the prior art, there is no device, which is not only suitable for the interaction experimental study of aquatic organisms, but also suitable for the interaction experimental study of plant roots.

Therefore, there is an urgent need for a multi-purpose interactive culture device, which not only facilitates the sufficient conduction and transmission of the chemosensory signal substances of two biological individuals (groups), but also can truly simulate the plant growth and interaction in the natural environment, and can be widely used in the interaction experiment of aquatic organisms and plant roots.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at solving one of the technical problem that exists among the prior art at least. Therefore, the utility model provides a novel culture apparatus for biological interaction, its seal is better, can effectively prevent the entering of impurity miscellaneous fungus, has avoided loaded down with trivial details complicated intercommunication operation among the prior art simultaneously, and it is more simple convenient to operate, has still shortened the transmission distance of different bottle internalization signal material, has increaseed the transmission sectional area to can be applied to aquatic organism and plant roots's interaction experiment, thereby application scope is wider, the usage is more.

According to the utility model discloses a novel culture apparatus for biological interaction, include:

the bottle comprises a plurality of bottle bodies, a plurality of sealing rings and a plurality of sealing rings, wherein the bottle bodies are provided with communicating pipelines, two adjacent bottle bodies are mutually communicated through the sleeve joint of the communicating pipelines, and an isolating layer is arranged between the two mutually communicated bottle bodies;

and the cover bodies are respectively covered on the bottle mouths of the bottle bodies in a one-to-one correspondence manner.

According to the novel culture device for biological interaction provided by the embodiment of the utility model, the mutual communication among a plurality of bottles is realized through the sleeve connection of the communication pipeline, so as to realize the contact among all parts of the interaction system and the transmission of chemical sensing objects among different bottles, and simultaneously, the isolation layers are utilized to avoid the direct contact among organisms in different bottles, thereby ensuring the accuracy of experimental results; in addition, as the communication is realized by adopting a sleeving way, the sealing performance is better, the sample leakage and the entrance of impurity and bacteria can be effectively prevented, the complex and complicated communication operation in the prior art is avoided, the operation is simpler and more convenient, the transmission distance of the chemical signal substances in different bottles is shortened, and the transmission sectional area is increased; in addition, when the cover body is installed, the device can be applied to interaction experiments of aquatic organisms, and can be applied to interaction experiments of plant roots after the cover body is detached, so that the device is wide in application range and multiple in application.

According to the utility model discloses an embodiment, two cup joint each other one in the intercommunication pipeline is equipped with the internal thread, two cup joint each other another in the intercommunication pipeline be equipped with interior screw-thread fit's external screw thread.

According to the utility model discloses an embodiment, the lid is equipped with fungi-proofing ventilation unit, fungi-proofing ventilation unit is used for gas exchange between the bottle is inside and the external world, blocks simultaneously that external microorganism gets into inside, fungi-proofing ventilation unit is the sponge layer, the sponge layer close joint in the mounting hole that the lid top was seted up.

According to the utility model discloses an embodiment, the bottle still is equipped with the sampling tube, the sampling tube is equipped with thief hole and valve, the valve is suitable for remove in order to open or close in the sampling tube the thief hole.

According to the utility model discloses an embodiment, two cup joint each other the stiff end of one in the intercommunication pipeline is equipped with the sealing washer, two cup joint each other the free end of another in the intercommunication pipeline support lean on in the sealing washer.

According to the utility model discloses an embodiment, the isolation layer passes through the sealing washer is fixed in the intercommunication pipeline.

According to the utility model discloses an embodiment, the intercommunication pipeline with bottle integrated into one piece, the intercommunication pipeline with the junction of bottle is formed with the buffer portion that the circular arc was handled.

According to the utility model discloses an embodiment, the internal diameter of bottleneck is 5.4cm to 5.8cm, two that communicate each other minimum interval between the bottle is 3.5cm to 3.8cm, the internal diameter of intercommunication pipeline is 5.3cm to 5.9 cm.

According to an embodiment of the utility model, the body of bottle is the ball shape.

According to one embodiment of the present invention, the isolation layer is a nylon mesh or a nylon filter membrane for avoiding contact between two plant roots in the communicating bottle bodies;

alternatively, the isolation layer is a permselective semi-permeable membrane or a qualitative filtration membrane for avoiding contact between two communicating aquatic organisms within the vial.

Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.

Drawings

In order to more clearly illustrate the technical solutions of the present invention or the prior art, the following briefly introduces the drawings required for the embodiments or the prior art descriptions, and obviously, the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic structural view of a novel culture apparatus for biological interaction according to the present invention;

FIG. 2 is a second schematic structural view of the novel culture apparatus for biological interaction provided by the present invention;

FIG. 3 is a schematic perspective view of the novel culture apparatus for biological interaction according to the present invention;

FIG. 4 is a second perspective view of the novel culture apparatus for biological interaction according to the present invention;

FIG. 5 is one of the schematic side views of the novel culture device for biological interaction provided by the present invention;

FIG. 6 is one of the schematic top views of the novel culture device for biological interaction provided by the present invention;

fig. 7 is a schematic structural diagram of the cover according to the present invention.

Reference numerals:

11. a first bottle body; 12. a second bottle body;

21. a first communicating pipe; 22. a second communicating conduit; 23. a buffer section;

3. an antibacterial ventilating cover body; 4. an isolation layer; 5. a sponge layer; 6. a sampling tube; 61. a sampling hole; 62. a valve; 7. and (5) sealing rings.

Detailed Description

To make the objects, technical solutions and advantages of the present invention clearer, the drawings of the present invention are combined to clearly and completely describe the technical solutions of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" should be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the embodiments of the present invention can be understood by those skilled in the art according to specific situations.

In embodiments of the invention, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of an embodiment of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.

The novel culture apparatus for biological interaction according to the embodiments of the present invention is described below with reference to the accompanying drawings.

As shown in fig. 1 to 7, a novel culture apparatus for biological interaction according to the present invention includes: a plurality of bottles and a plurality of caps 3.

The bottle is equipped with the intercommunication pipeline, cup joints through the intercommunication pipeline between two adjacent bottles in order to communicate each other, is equipped with isolation layer 4 between the two bottle that communicate each other. The plurality of cover bodies 3 are respectively covered on the bottle mouths of the plurality of bottle bodies in a one-to-one correspondence manner.

According to the novel culture device for biological interaction provided by the embodiment of the utility model, the mutual communication among a plurality of bottles is realized through the sleeve connection of the communication pipeline, so as to realize the contact among all parts of the interaction system and the transmission of chemical sensing objects among different bottles, and simultaneously, the isolation layer 4 is utilized to avoid the direct contact among organisms in different bottles, thereby ensuring the accuracy of experimental results; in addition, as the communication is realized by adopting a sleeving way, the sealing performance is better, the leakage of a sample and the entrance of impurity and bacteria can be effectively prevented, the complex and complicated communication operation in the prior art is avoided, the operation is simpler and more convenient, the transmission distance of chemical signal substances in different bottles is shortened, and the transmission sectional area is increased; besides, when the cover body 3 is installed, the device can be applied to interaction experiments of aquatic organisms, and can be applied to interaction experiments of plant roots after the cover body 3 is detached, so that the device is wide in application range and multiple in application.

In the prior art, the novel culture device for biological interaction usually adopts an auxiliary tool (such as a clamp) to mutually communicate the communication ports of the two bottle bodies so as to realize the transmission of chemical sensitive substances between different bottle bodies, and has the disadvantages of complicated and trivial communication mechanism, inconvenient communication operation, poor tightness after communication and unstable standing of the device due to different planes at the bottoms of the two bottle bodies after communication due to more fine and broken parts.

In order to solve the technical problem, the utility model discloses a novel culture apparatus for biological interaction adopts the mode that the intercommunication pipeline cup jointed each other to realize the intercommunication between the different bottles, has saved loaded down with trivial details intercommunication operation among the prior art to guaranteed the seal simultaneously, realized cup jointing completely along the rotatory a week of screw thread, also guaranteed two bottle bottom departments coplanar after the intercommunication, guaranteed that the device stands stably.

According to the embodiment of the present invention, the same or different organisms or groups of organisms can be placed in different bottles, and the organisms or groups of organisms can be bacteria, fungi, protozoa, aquatic plants, terrestrial plants, etc., so that the present invention can be widely applied to the interaction culture research mediated by the chemosensory signal substances between any multiple organisms or multiple groups of organisms, for example, the present invention can be applied to the interaction research between multiple or multiple groups of small aquatic organisms, and at this time, the cover body 3 is covered on the bottle body to ensure that external microorganisms do not enter the interior while ensuring the air permeability; also can be applied to the interaction research between the medium and small-size plant roots under the soil cultivation, dismantle the lid 3 this moment in order to guarantee the overground part's of plant normal growth, avoid the overground part of plant to receive blockking of lid 3 for the plant can freely upwards grow.

According to the utility model discloses an embodiment, above-mentioned "cup joint" indicates: one communicating pipeline is sleeved outside the other communicating pipeline to realize the communication and the fixation between the two communicating pipelines.

The cross sectional shape of intercommunication pipeline can be for circular, rectangle or polygon, and intercommunication pipeline and bottle can be integrated into one piece, and intercommunication pipeline also can be split type design with the bottle, the utility model discloses do not specially prescribe a limit to the concrete structure of intercommunication pipeline, as long as can cup joint each other between the intercommunication pipeline of two adjacent bottles can.

As shown in fig. 1, according to an embodiment of the present invention, the two bottles are respectively the first bottle 11 and the second bottle 12, the outer surface of the first bottle 11 is provided with a first communicating opening, the first communicating opening is connected with the first communicating pipe 21, the outer surface of the second bottle 12 is provided with a second communicating opening, the second communicating opening is connected with the second communicating pipe 22, and the first communicating pipe 21 is disposed inside the second communicating pipe 22.

According to the utility model discloses a further embodiment, the bottle also can be more than two quantity, is equipped with a plurality of intercommunication mouths on one of them bottle, and above-mentioned a plurality of intercommunication mouths are connected with a plurality of intercommunication pipeline one-to-one respectively, and this bottle communicates each other with a plurality of other bottles through above-mentioned a plurality of intercommunication pipelines.

According to the utility model discloses an embodiment, one in two communicating pipes that cup joint each other is equipped with the internal thread, and another in two communicating pipes that cup joint each other is equipped with the external screw thread with interior screw-thread fit. Like this, adopt screw-thread fit's mode to connect and to make whole novel culture apparatus's leakproofness better to the structure is also more stable firm, avoids breaking away from between two communicating pipes.

As shown in fig. 1, the outer peripheral surface of the first communicating pipe 21 is provided with an external thread which surrounds the entire circumference of the outer peripheral surface of the first communicating pipe 21 and extends from one end of the first communicating pipe 21 to the other end, the inner peripheral surface of the second communicating pipe 22 is provided with an internal thread which surrounds the entire circumference of the inner peripheral surface of the second communicating pipe 22 and extends from one end of the second communicating pipe 22 to the other end, and a user can realize the screw-thread fit between the first communicating pipe 21 and the second communicating pipe 22 by directly screwing the first communicating pipe 21 and the second communicating pipe 22.

Preferably, the first communicating channel 21 is made of glass, and the second communicating channel 22 is made of transparent polyethylene. Thus, the second communicating pipe 22 has better toughness, elasticity, heat resistance and impact resistance, and can be tightly attached to the pipe wall of the first communicating pipe 21 during sleeving to achieve better sealing effect.

According to the utility model discloses an embodiment, lid 3 is equipped with anti-bacterial ventilation device, and anti-bacterial ventilation device is used for the gas exchange between the bottle is inside and the external world, blocks simultaneously that outside microorganism gets into inside. Like this, can also guarantee inside and external gas exchange and the no miscellaneous nature of bottle when having guaranteed the leakproofness of bottle to guarantee the inside biological normal growth of bottle.

Wherein, the anti-bacterial ventilation device can be the air vent, breather pipe, ventilation medium isotructure, the utility model discloses do not specially prescribe a limit to here, as long as the anti-bacterial ventilation device can realize the inside gas exchange with the external world of bottle to it can not get into inside to block outside microorganism.

In an embodiment of the present invention, the anti-bacterial ventilation device can be an anti-bacterial ventilation hole, which is formed at the top of the cover body 3 and is multiple, and the diameters of the multiple anti-bacterial ventilation holes are kept in proper sizes to avoid the entrance of impurities and bacteria while ensuring gas exchange. Furthermore, a filter screen can be arranged in the vent hole to fully prevent impurities and bacteria from entering the bottle body.

As shown in fig. 4, in another embodiment of the present invention, the antibacterial ventilating device is a sponge layer 5, and the sponge layer 5 is closely connected in the mounting hole formed at the top of the cover body 3. Thus, the sponge layer 5 has the functions of ventilation and bacteria prevention, and has the advantages of simple structure, low cost and convenient replacement. Wherein the sponge layer 5 can be a micro-aperture sponge layer 5.

In an embodiment of the present invention, the cover body 3 is a special antibacterial ventilating bottle cap, which has a flat top circular cap shape in appearance, the top surface of the cover body 3 is circular and has a diameter of 7.2cm to 7.8cm, the cover body 3 is provided with a mounting hole, the diameter of the mounting hole is the same as the outer diameter of the bottle mouth, and a sponge layer 5 is provided in the mounting hole, which has the effects of ventilation and antibacterial.

The top surface of lid 3 is made by the duroplasts of polypropylene material, and the side eaves of lid 3 is made by high temperature resistant silica gel material, and the flap internal diameter of lid 3 is 5.6cm to 6.0cm, and the flap internal diameter of lid 3 is the same with the bottleneck external diameter. Because silica gel has certain elasticity, so lid 3 can with the protruding seamless combination of bottleneck periphery when lid 3 is established to the lid, lid 3's dismantlement is also more convenient. Because the material quality of lid 3 is high temperature resistant material, consequently can carry out high temperature sterilization to lid 3 in order to guarantee the accuracy of experiment.

In the prior art, in the scheme of adopting the dialysis membrane bag to carry out the interaction experiment, because the structure restriction of dialysis membrane bag itself, therefore can't realize the multiple time point interval sample in the biological growth period to the dialysis membrane bag sample difficulty of soaking just causes the pollution easily, can lead to the interior biological growth of bag to receive the interference of other factors. In the scheme of adopting the communicated culture bottles, the sampling is usually carried out through the bottle mouth without a special sampling port, so that the sampling operation is complicated and the pollution is easily caused.

In order to solve the above technical problem, according to the utility model discloses an embodiment, the bottle still is equipped with sampling tube 6, and sampling tube 6 is equipped with thief hole 61 and valve 62, and valve 62 is suitable for and removes in order to open or close thief hole 61 in sampling tube 6. Like this, can realize the sample through simple removal valve 62 alright, easy operation is convenient, owing to under the state of not taking a sample, the thief hole 61 is in the closed condition, consequently can guarantee the leakproofness of whole device, avoids the bottle inside to receive the pollution.

As shown in fig. 1, the surface of bottle is located to sampling tube 6, sampling tube 6 is "L" type, the lower half section level of sampling tube 6 sets up in order to form the horizontal segment, the vertical setting of the first half section of sampling tube 6 is in order to form vertical section, thief hole 61 has been seted up to the lower extreme of vertical section, valve 62 is a piston that has the action bars, the movably cooperation of piston is in vertical section, the user can be through reciprocating the piston in order to realize opening and closing of thief hole 61, can also control the size of thief hole 61 opening degree, thereby the flow rate of the sample of control from thief hole 61 outflow.

As shown in fig. 1, according to an embodiment of the present invention, a sealing ring 7 is disposed between two communicating pipes that are sleeved with each other. Therefore, the tightness between the two mutually sleeved communication pipelines can be ensured, and the tightness of the whole device is further ensured. Wherein, sealing washer 7 can be rubber circle or silica gel circle, can guarantee the seamless intercommunication between the bottle after the twist closes the intercommunication, and the seal is good. It should be noted that the sealing ring 7 may also be a sealing ring made of other materials, as long as the sealing ring 7 has good material stability and can achieve a sealing effect.

As shown in fig. 2, further, the second communicating channel 22 is sleeved outside the first communicating channel 21, the diameter of the sealing ring 7 is the same as the inner diameter of the first communicating channel 21, and after the first communicating channel 21 and the second communicating channel 22 are sleeved with each other, the bottom surface of the first bottle 11 and the bottom surface of the second bottle 12 are in the same plane, so as to ensure stable standing.

According to the utility model discloses a another embodiment, the stiff end of one in two communicating pipes that cup joint each other (communicating pipe and bottle intercommunication mouth department promptly) is equipped with sealing washer 7, and the free end of another in two communicating pipes that cup joint each other (communicating pipe mouth promptly) supports and leans on in sealing washer 7.

According to the utility model discloses an embodiment, isolation layer 4 is located between the pipeline mouth and the second intercommunication pipeline 22 and the bottle intercommunication mouth department of first intercommunication pipeline 21, and isolation layer 4 is fixed through sealing washer 7. Thus, the installation of the isolation layer 4 is more convenient and stable.

As shown in fig. 1, the first bottle 11 is located at the left side of the second bottle 12, the first communicating channel 21 is sleeved at the inner side of the second communicating channel 22, the sealing ring 7 is located at the right end of the second communicating channel 22, and the sealing ring 7 fixes the isolation layer 4 at the channel opening at the right end of the second communicating channel 22.

In the prior art, in order to avoid direct contact of organisms in different bottles, a communicating pipe for communicating the bottles is usually designed to have a smaller caliber and a longer length, so that the contact area of an interaction system between different bottles is small, and the object sensing transmission distance is long, thereby influencing the accuracy of an interaction experimental result.

In order to solve the above technical problem, according to some embodiments of the present invention, the inner diameter of the bottle mouth of the bottle body is 5.4cm to 5.8cm, the minimum distance between two adjacent bottle bodies is 3.5cm to 3.8cm, and the inner diameter of the communicating pipe is 5.3cm to 5.9 cm.

The bottle body is designed into a wide-mouth bottle with a large caliber, on one hand, the barrier-free entering of a water culture medium, a soil culture medium and an individual organism or a group of organisms in the construction process of an interaction experiment system can be met, on the other hand, the overground part or the overwater part (namely stems and leaves) of the medium and small plants can grow upwards freely, the situation that the growth of the plants is limited due to too small bottle openings is prevented, and meanwhile, the convenient operation of soil collection and sampling in the soil culture process can also be met.

It should be noted that, on the one hand, because the utility model discloses a novel culture apparatus adopts isolation layer 4 to realize the non-direct contact of the internal biology of different bottles, therefore the intercommunication pipeline need not to be set up to long and thin structure, and on the other hand, because two intercommunication pipelines are connected through the mode that cup joints, therefore the total length after two intercommunication pipelines connect only is equivalent to the length of single intercommunication pipeline, and the transmission distance of changing the signal material of feeling shortens greatly. In conclusion, the interval between two adjacent bottles can be very small, specifically can be 3.5cm to 3.8cm, that is to say, the transmission distance of the chemical signal substance in different bottles is greatly shortened, and the transmission sectional area is increased, so that the problem of poor accuracy of the interaction simulation experiment caused by overlong transmission distance and undersize section of the chemical signal substance is effectively avoided.

The container convenient for the interaction of two or more than two crop roots is disclosed in the prior art, the center of the pot body is provided with the crossed nylon net filter membrane, the pot body is divided into four small chamber spaces, the winding and the crossed growth of the individual root systems of different crops can be effectively prevented, and the independent analysis of the variation of the content of nutrient components in the individual rhizosphere of a certain crop or the rhizosphere soil and the interaction relation among the root systems of different crops are facilitated. However, the growth chamber space of each crop in the container is very narrow and is a fan-shaped column, which does not meet the natural normal growth condition and shape distribution condition of the root system under the natural environment. Other root boxes with similar designs in the prior art are mostly square, and have the same defect of limiting root growth.

In order to solve the above technical problem, according to the utility model discloses an embodiment, the bottle main part is the ball shape. Thus, aiming at the interaction experiment among aquatic organisms, a large volume of liquid culture solution can be accommodated in the bottle body; aiming at the interaction experiment between the plant root systems, sufficient transverse and longitudinal spaces can be provided in the bottle body, and the normal and sufficient growth and morphological distribution of the plant root systems are met.

Specifically, the bottle includes bottleneck, bottleneck and body, and the body main part of bottle is the ball shape, and the volume of bottle is 360mL to 400 mL.

According to one embodiment of the present invention, the isolation layer 4 is a nylon mesh or a nylon filter membrane for avoiding contact between plant roots in two communicated bottles; alternatively, the isolation layer 4 is a permselective semi-permeable membrane or qualitative filtration membrane for avoiding contact between aquatic organisms in two communicating vials.

Therefore, the normal and smooth transmission of the chemical sensing signal substance is ensured while the indirect contact of organisms in different bottles is ensured. For example, the isolation layer 4 may employ a nylon filter with a pore size of 48 μm for the interaction isolation between plant roots, or the isolation layer 4 may employ a qualitative filter with a pore size of 0.45 μm for the interaction isolation between aquatic organisms. Wherein, the aquatic organisms refer to aquatic microorganisms (such as bacteria and algae) and micro-animals (such as paramecium) which are not easy to separate technically (man-made or mechanical).

According to an embodiment of the utility model, the bottle body is a glass piece, and the whole novel culture device (except the cover body) is a transparent piece.

As shown in fig. 4, the communication channel is integrally formed with the bottle, and a buffer portion 23 is formed at the junction of the communication channel and the bottle.

For example, the wall thickness of the bottle body is 0.2cm, the thickness of the communication pipeline is 0.4cm, the thickness of the communication pipeline is gradually reduced from 0.4cm to 0.2cm from the joint of the communication pipeline and the bottle body to the bottle body of the bottle body, and the joint of the communication pipeline and the bottle body is processed by circular arc treatment (namely, the intersection line of a cylinder and a sphere is weakened), so that the two communication pipelines are ensured to keep a stress dispersion state in the screwing process, and the bottle body and the communication pipeline are prevented from cracking and breaking when being subjected to torque force or impact vibration.

The experimental device aims to solve the problems that in the prior art, in the interaction research of aquatic organisms (such as microorganisms and small protozoa), the growth environment among the interacting organisms is different, the conductance of chemosensory signals is not smooth and uniform, the sampling operation is complex and difficult, the system is easy to pollute, bacteria and air are prevented, and the experimental device for simulating natural growth of the root system of the plant interaction root box is lack.

The utility model provides a novel culture apparatus for biological interaction can wide application in aquatic microorganism, small-size protozoan, aquatic plant, terrestrial plant root system biological metabolism sense thing mediation's interaction experiment, can ensure that the interaction system contact is abundant, the thing conduction of changing is exchanged smoothly, has seamless airtight intercommunication concurrently, sample convenient operation is difficult for polluting, fungi-proofing ventilative type nature is good, more advantage such as true simulation actual environment is to biological effect.

Fig. 1 to 7 depict one embodiment of the novel culture device for biological interaction according to the present invention.

The novel culture device comprises a first bottle body 11, a second bottle body 12 and two cover bodies 3, wherein the cover bodies 3 are specially made antibacterial ventilating bottle caps. The first bottle body 11 and the second bottle body 12 are screwed through the first communicating pipeline 21 and the second communicating pipeline 22, two sealing rings 7 are arranged at the joint of the second communicating pipeline 22 and the second bottle body 12, the sealing rings 7 are thin silica gel rings, the two sealing rings 7 clamp the isolating layer 4 in the middle, and the isolating layer 4 can be a qualitative semipermeable membrane, a filter membrane (used for culturing aquatic microorganisms and small animals) or a nylon net filter membrane (used for culturing medium and small plants). The first bottle 11 is located on the left side of the second bottle 12, the left side of the first bottle 11 and the right side of the second bottle 12 are respectively provided with a sampling tube 6, and the sampling tube 6 controls the sampling amount by moving the valve 62 up and down by the user.

Specifically, the bottle body, the first communicating pipeline 21 and the sampling tube 6 are made of glass, the glass is transparent and has good chemical stability, and the second communicating pipeline 22 is made of polyethylene and is also made of transparent material, so that the organisms can conveniently carry out normal photosynthesis. It should be noted that the bottle bodies mentioned hereinafter refer to the first bottle body 11 and the second bottle body 12 unless otherwise specified. The valve 62 is a piston with an operating rod, the piston is a square silica gel body matched with the inner diameter of the sampling tube 6, and the operating rod is made of hard plastic with polypropylene as a main raw material and can resist high-temperature sterilization.

Preferably, the inner diameter of the bottle mouth of the bottle body is 5.6cm, the outer diameter of the bottle mouth is 5.9cm, the length of the bottle neck is 2.4cm, and therefore the overground part of the plant can freely and fully grow upwards conveniently. The diameter at the top of lid 3 is 7.5cm, and the internal diameter of 3 gaits of lid is 5.9cm, is equipped with antibiotic breather in the mounting hole at lid 3 top, and antibiotic breather is the sponge layer 5 of thickness 0.3 cm.

Preferably, the bottle body is in a spherical shape, and the diameter of the bottle body is 9 cm. The sampling tube 6 comprises a horizontal section and a vertical section, the length of the horizontal section is 2.5cm, the length of the vertical section is 2.2cm, and the opening of the sampling tube 6 is square and the side length is 1 cm.

Preferably, the inner diameter of the first communicating channel 21 is 5.6cm, the first communicating channel 21 and the second communicating channel 22 are both horizontally arranged, and after the first communicating channel 21 and the second communicating channel 22 are twisted together, the distance between the first bottle body 11 and the second bottle body 12 is 3.5 cm.

The utility model discloses a novel cultivation device for biological interaction's working method as follows: the first communicating duct 21 and the second communicating duct 22 are seamlessly communicated by screwing, so that the first bottle body 11 and the second bottle body 12 are communicated. When used for aquatic organism culture, the isolation layer 4 is a semi-permeable membrane filter or a qualitative filter with a pore diameter of 0.45 μm, so that microorganisms and small animal cells can not pass through the membrane, but chemosensory signal substances can pass through the membrane; when the filter is used for culturing aquatic plants or terrestrial plants, the isolating layer 4 is a nylon net filter membrane with the hole diameter of 48 mu m, the roots of the plants cannot pass through the isolating layer 4 and cannot be wound and contacted, but root exudates can pass through the roots.

During the interaction culture of aquatic organisms, the valve 62 is closed, liquid culture medium with aquatic organisms is injected into the bottle through the bottle opening of the bottle body until the liquid culture medium submerges the upper edge of the communicating pipeline, a specially-made antibacterial ventilating cover body is covered for interaction culture, and a sample is taken by moving the valve 62.

When the soil-filling bottle is used for cultivating medium and small plants, the soil is filled in the bottle body of the whole bottle body, so that the root system can fully grow in the transverse direction and the longitudinal direction.

To sum up, according to the utility model discloses a novel culture apparatus for biological interaction of a specific embodiment mainly is made by printing opacity glass material, cultivates the module etc. including the module of ventilating, intercommunication module, sample collection module, storage, uses simple operation, green high-efficient, can effectively prevent miscellaneous fungus entering inside in cultivateing the process to can realize cultivateing the aseptic ization sample of in-process.

According to the utility model discloses a novel culture apparatus for biological interaction has constructed sterile environment in cultivateing whole journey, and when avoiding two kinds of biology or two bodies of the same kind of biology or the direct contact of clan, it exchanges to allow to change to feel the abundant transmission of signal material, can solve the problem that the signal material conduction transmission is insufficient to change of two biological individuality or two biological clans of interaction cultivation in-process, the intercommunication butt joint complex operation that can also solve the blake bottle is poor and the butt joint effect, the not good problem of seal, can also guarantee all be in the coplanar in the bottom of first bottle 11 and second bottle 12 after the butt joint, thereby guarantee that novel culture apparatus stands stably.

In addition, the sampling tube 6 can solve the problem that the sampling is inconvenient and easy to pollute, and the spherical bottle body can solve the problem that the growth of the plant root system is limited, so that the result of the interaction experiment is more accurate.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention in its corresponding aspects. Therefore, any modification, equivalent replacement, improvement, etc. (including but not limited to any modification, replacement, improvement, etc. of material, appearance, shape, size) within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. A novel culture device for biological interaction, comprising:

the bottle comprises a plurality of bottle bodies, a plurality of sealing rings and a plurality of sealing rings, wherein the bottle bodies are provided with communicating pipelines, two adjacent bottle bodies are mutually communicated through the sleeve joint of the communicating pipelines, and an isolating layer is arranged between the two mutually communicated bottle bodies;

and the cover bodies are respectively covered on the bottle mouths of the bottle bodies in a one-to-one correspondence manner.

2. The novel culture device for biological interaction of claim 1, wherein one of the two communicating conduits that are nested with each other is provided with an internal thread, and the other of the two communicating conduits that are nested with each other is provided with an external thread that is engaged with the internal thread.

3. The novel culture device for biological interaction as claimed in claim 1, wherein the cover body is provided with an antibacterial ventilation device for gas exchange between the inside of the bottle body and the outside while blocking external microorganisms from entering the inside, and the antibacterial ventilation device is a sponge layer which is closely bonded in a mounting hole formed at the top of the cover body.

4. The novel culture device for biological interaction of claim 1, wherein the flask is further provided with a sampling tube provided with a sampling hole and a valve adapted to move within the sampling tube to open or close the sampling hole.

5. The novel culture device for biological interaction according to claim 1, wherein a fixed end of one of the two communicating conduits that are nested into each other is provided with a sealing ring, and a free end of the other of the two communicating conduits that are nested into each other abuts against the sealing ring.

6. The novel culture device for biological interaction according to claim 5, wherein the isolation layer is fixed in the communication channel by the sealing ring.

7. The novel culture device for biological interaction according to any one of claims 1 to 6, wherein the communication channel is integrally formed with the bottle body, and a buffer portion for rounding is formed at a junction of the communication channel and the bottle body.

8. The novel culture device for biological interaction according to any one of claims 1 to 6, wherein the inner diameter of the bottle mouth is 5.4cm to 5.8cm, the minimum distance between two mutually communicating bottle bodies is 3.5cm to 3.8cm, and the inner diameter of the communication pipe is 5.3cm to 5.9 cm.

9. The novel culture device for biological interaction of any one of claims 1 to 6, wherein the body of the bottle is spherical.

10. The novel culture device for biological interaction as claimed in any one of claims 1 to 6, wherein the isolation layer is a nylon mesh or a nylon filter for avoiding contact between plant roots in two communicating flasks;

alternatively, the isolation layer is a permselective semi-permeable membrane or a qualitative filtration membrane for avoiding contact between two communicating aquatic organisms within the vial.

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