CN210620775U - Device for in-situ culture or actinomycete capture of soil environment microorganisms - Google Patents
Device for in-situ culture or actinomycete capture of soil environment microorganisms Download PDFInfo
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- CN210620775U CN210620775U CN201921417157.9U CN201921417157U CN210620775U CN 210620775 U CN210620775 U CN 210620775U CN 201921417157 U CN201921417157 U CN 201921417157U CN 210620775 U CN210620775 U CN 210620775U
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- filter membrane
- base
- sealing washer
- actinomycete
- soil environment
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Abstract
The utility model provides a pair of a device that is used for soil environment microorganism normal position to cultivate or actinomycete to catch includes the box body, including the agar culture medium, agar culture medium upper portion is equipped with first filter membrane, and agar culture medium lower part is equipped with the second filter membrane, is equipped with first sealing washer on the first filter membrane, and the second filter membrane has the second sealing washer, is equipped with the lid on the first sealing washer, and the second sealing washer has and the base, lid and base suit setting. The utility model is used for device that soil environment microorganism normal position was cultivateed or actinomycetes was caught, the lower cover closes design and the sealed application of silica gel gasket with the wrong of going to the bottom for originally paste inside the device is shifted to at outside filter membrane, the process of pasting of glue has been avoided, the time of experimental operation has been simplified, the pollution rate has been reduced, the influence of glue volatility or water-soluble material to the cultivation that has caused has been avoided, three big shortcomings that exist for a long time in the aspect of microorganism normal position cultivation and actinomycetes are caught have been solved, have extensive suitability in the microorganism cultivation field.
Description
Technical Field
The utility model relates to a cultivate the microbial cultivation field, it is specific, the utility model relates to a cultivate microbial cultivation technical field.
Background
Microorganisms in the natural environment are characterized by high diversity, and 99% of microorganisms are still "non-culturable" using traditional methods, and the determination of new microbial species and functions is still an important task at present. Under the condition of laboratory culture, the microorganisms to be cultured are simply separated from other related microorganism groups, the information communication between the species is blocked, and the microorganisms cannot grow due to the lack of necessary growth factors and signal molecules, so that the microorganisms are represented as being unculturable.
In situ culture and isolation, which simulates the natural environment of the target microorganism, is a basic strategy for researchers. Kaeberlein et al, which simulates the natural growth environment of marine microorganisms, designed a self-made culture apparatus called a diffusion growth box (diffusion growth chamber) and used this apparatus to separate and culture microorganisms.
The diffusion box is formed by sticking filter membranes with the aperture of 0.03 mu m on two sides of an annular stainless steel gasket by using glue, the filter membranes allow beneficial small-molecule metabolic substances and active substances generated by microorganisms inside and outside the ring to freely enter and exit a small chamber through the membranes, chemical substances are communicated with the environment in the box, and meanwhile, microbial cells cannot escape through the filter membranes (the aperture of the filter membranes is smaller than the diameter of the microorganisms), so that the primordial environment culture becomes possible.
The current related devices have the following three major drawbacks. The first is that the filter membrane needs to be glued to the device. The pasting process is complicated and time-consuming. The second disadvantage is that when the device is placed in a relatively humid environment for in-situ culture, the filter membrane adhered with glue is easy to fall off, causing pollution and causing culture failure. Thirdly, it is difficult to select glue without pungent taste, volatile or water-soluble substances, so as not to affect the culture.
SUMMERY OF THE UTILITY MODEL
The utility model has the advantages of complex operation and long time consumption in the process of pasting the filter membrane of the prior device; the filter membrane adhered by the in-situ culture glue is easy to fall off when placed in a humid environment, so that pollution is caused, and the culture fails; select glue more difficult, can not have the taste of irritability, volatile or water-soluble material to influence the technical problem of cultivateing, the utility model provides a device that is used for soil environment microorganism normal position to cultivate or the actinomycete is caught, the lower cover closes the application that design and silica gel gasket are sealed with the twisting of going to the bottom. Make originally paste inside outside filter membrane shifts the device, avoided the process of pasting of glue, simplified the time of experimental operation, reduced the pollution rate, avoided the influence that glue volatility or water-soluble material cause the cultivation, have extensive suitability in microorganism normal position cultivation field.
In order to achieve the above object, the utility model provides a following technical scheme:
the utility model provides a pair of a device that is used for soil environment microorganism normal position to cultivate or actinomycete to catch includes the box body, including the agar culture medium, agar culture medium upper portion is equipped with first filter membrane, and agar culture medium lower part is equipped with the second filter membrane, is equipped with first sealing washer on the first filter membrane, and the second filter membrane has the second sealing washer, is equipped with the lid on the first sealing washer, and the second sealing washer has and the base, lid and base suit setting.
The utility model discloses in, be equipped with the internal thread in the lid, be equipped with the external screw thread on the base, internal thread and external screw thread suit setting.
The utility model discloses in, second filter membrane thickness is greater than first filter membrane.
The utility model discloses in, base, lid, first sealing washer and second sealing washer all are the annular.
The utility model discloses in, first sealing washer and second sealing washer are elastic sealing ring.
The utility model discloses in, the base cross section is the T font that two symmetries set up.
The utility model discloses in, the inner ring size of second sealing washer and base external screw thread suits, and the outer loop size of first sealing washer and base external screw thread suits the setting.
The utility model discloses in, the thickness sum and the base height phase adaptation setting of agar-agar culture medium, second filter membrane and second sealing washer.
The utility model discloses a following beneficial effect has:
the utility model provides a pair of a device that is used for soil environment microorganism normal position to cultivate or actinomycetes to catch, the lower cover closes design and the sealed application of silica gel gasket with the wrong of going to the bottom for originally paste inside the device is shifted to at outside filter membrane, the process of pasting of glue has been avoided, the time of experimental operation has been simplified, the pollution rate has been reduced, the influence that glue volatility or water-soluble material cause to the cultivation has been avoided, three big shortcomings that exist for a long time in the aspect of microorganism normal position cultivation and actinomycetes are caught have been solved, have extensive suitability in the microorganism cultivation field.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive labor.
Fig. 1 shows the structure of the present invention.
Fig. 2 is a schematic sectional view of the present invention.
Fig. 3 shows an explosion diagram of the present invention.
In FIGS. 1-3: 1-base, 2-cover, 3-first filter membrane, 4-first sealing ring, 5-agar culture medium, 6-second filter membrane, 7-second sealing ring.
Detailed Description
The following description of the present invention will be made in further detail with reference to the accompanying drawings 1 to 3 and examples, but the present invention is not limited to the following examples.
In the present invention, for the sake of convenience and description, the description of the relative position relationship of the components of a device for in situ culture of soil environment microorganisms or capture of actinomycetes is described according to the layout of the attached fig. 1, as follows: the positional relationship of up, down, left, right, etc. is determined according to the layout direction of fig. 1.
The utility model discloses well used base 1, lid 2, first filter membrane 3, first sealing washer 4, agar culture medium 5, second filter membrane 6, second sealing washer 7 etc. can both purchase through the market approach and obtain.
The utility model provides a pair of a device that is used for soil environment microorganism normal position to cultivate or actinomycete to catch includes the box body, including agar culture medium 5, 5 upper portions of agar culture medium are equipped with first filter membrane 3, and 5 lower parts of agar culture medium are equipped with second filter membrane 6, are equipped with first sealing washer 4 on the first filter membrane 3, and second filter membrane 6 has second sealing washer 7, is equipped with lid 2 on the first sealing washer 4, and second sealing washer 7 has and base 1, and lid 2 and base 1 suit sets up.
The utility model discloses in, be equipped with the internal thread in the lid 2, be equipped with the external screw thread on the base 1, internal thread and external screw thread suit setting.
In the utility model, the thickness of the second filter membrane 6 is larger than that of the first filter membrane 3.
Preferably, the first filter 3 is a 0.03 μm filter and the second filter 6 is a 0.2 μm filter.
The utility model discloses in, base 1, lid 2, first sealing washer 4 and second sealing washer 7 all are the annular.
The utility model discloses in, first sealing washer 4 and second sealing washer 7 are elastic seal circle.
Preferably, the first sealing ring 4 and the second sealing ring 7 are sealing rubber rings.
The utility model discloses in, base 1 cross section is the T font that two symmetries set up.
The utility model discloses in, the inner ring size of second sealing washer 7 and 1 external screw thread of base suits, and the outer ring size of first sealing washer 4 and 1 external screw thread of base suits the setting.
In the utility model, the thickness sum of the agar culture medium 5, the second filter membrane 6 and the second sealing ring 7 is highly adaptive to the setting of the base 1.
The utility model is used for device that soil environment microorganism normal position was cultivateed or actinomycete was caught, including upper cover 2 and lower floor's base 1, upper cover 2 is high 0.6cm, and diameter 6cm is middle is round fretwork, and the round diameter of fretwork 3.8 cm. The lower layer is of a base 1 structure, the height of the lower layer is 0.6cm, the diameter of the lower layer is 6.6cm, the middle of the lower layer is a circular hollow part, and the diameter of the hollow part is 2.4 cm. The cover body 2 and the base 1 are screwed together by a turnbuckle. In the using process, a first filter membrane 3 and a second filter membrane 6 are sequentially arranged on the inner surfaces of the cover body 2 and the base 1, and a solid agar culture medium 5 is poured in the middle.
Use 1: if the microorganism is cultured in the primary environment, a microorganism sample is added on the surface of the agar culture medium 5, then the first filter membrane 3 is covered, then the first sealing ring 4 is covered, and finally the cover body 2 is screwed. The device is placed in a microbial environment for culturing.
Use 2: if the filter is used for collecting actinomycetes in an environment, the first filter membrane 3 is placed on the upper surface of the agar culture medium 5, and the cover body 2 is screwed after the first sealing ring 4 is covered. And (3) placing the device in a microbial protogenic environment for collecting and culturing actinomycetes.
The utility model discloses the lower cover closes design and the sealed application of silica gel gasket with the twisting of going to the bottom for originally paste inside the filter membrane of outside shifts the device, avoided pasting of glue process, reduced the pollution rate, avoided glue volatility or water-soluble material to the influence of cultivateing and causing, cultivate and the actinomycete catches the cultivation to the normal position and all have very important effect.
The technical solutions provided by the embodiments of the present invention are described in detail above, and the principles and embodiments of the present invention are explained herein by using specific examples, and the descriptions of the above embodiments are only applicable to help understand the principles of the embodiments of the present invention; meanwhile, for a person skilled in the art, according to the embodiments of the present invention, there may be variations in the specific implementation manners and application ranges, and in summary, the content of the description should not be construed as a limitation to the present invention.
Claims (8)
1. A device for in situ culture or actinomycete capture of soil environment microorganisms comprises an agar culture medium and is characterized in that: agar culture medium upper portion is equipped with first filter membrane, and agar culture medium lower part is equipped with the second filter membrane, is equipped with first sealing washer on the first filter membrane, and the second filter membrane has the second sealing washer, is equipped with the lid on the first sealing washer, and the second sealing washer has and the base, lid and base suit setting.
2. The device for in situ culture or actinomycete capture of microorganisms in a soil environment as claimed in claim 1, wherein the cover has internal threads and the base has external threads, and the internal threads and the external threads are correspondingly arranged.
3. The device for in situ culture or actinomycete capture of microorganisms in a soil environment as claimed in claim 1 wherein the second filter is thicker than the first filter.
4. The apparatus according to claim 1, wherein the base, the cover, the first sealing ring and the second sealing ring are annular.
5. The device for in situ culture or actinomycete capture of a soil environment microorganism as claimed in claim 1 wherein the first and second sealing rings are elastomeric sealing rings.
6. The device for in situ culture or actinomycete capture of microorganisms in a soil environment as claimed in claim 1, wherein the base has a cross section in the form of two symmetrically disposed tees.
7. The device for in situ culture or actinomycete capture of microorganisms in a soil environment as set forth in claim 1, wherein the second seal ring is adapted to the inner ring of the external thread of the base and the first seal ring is adapted to the outer ring of the external thread of the base.
8. The device for in situ culture or actinomycete capture of microorganisms in a soil environment as set forth in claim 1, wherein the sum of the thicknesses of the agar medium, the second filter membrane and the second sealing ring is set in accordance with the height of the base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921417157.9U CN210620775U (en) | 2019-08-29 | 2019-08-29 | Device for in-situ culture or actinomycete capture of soil environment microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921417157.9U CN210620775U (en) | 2019-08-29 | 2019-08-29 | Device for in-situ culture or actinomycete capture of soil environment microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210620775U true CN210620775U (en) | 2020-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921417157.9U Expired - Fee Related CN210620775U (en) | 2019-08-29 | 2019-08-29 | Device for in-situ culture or actinomycete capture of soil environment microorganisms |
Country Status (1)
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|---|---|
| CN (1) | CN210620775U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112630246A (en) * | 2020-11-30 | 2021-04-09 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for capturing and imaging long and medium linear microorganisms in sediment |
-
2019
- 2019-08-29 CN CN201921417157.9U patent/CN210620775U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112630246A (en) * | 2020-11-30 | 2021-04-09 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for capturing and imaging long and medium linear microorganisms in sediment |
| CN112630246B (en) * | 2020-11-30 | 2022-12-23 | 广东省微生物研究所（广东省微生物分析检测中心） | Method for capturing and imaging long and medium linear microorganisms in sediment |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200526 Termination date: 20210829 |