CN105820952A - Bioreactor for cell perfusion culture - Google Patents
Bioreactor for cell perfusion culture Download PDFInfo
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- 230000010412 perfusion Effects 0.000 title abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 38
- 238000003756 stirring Methods 0.000 claims abstract description 21
- 239000001963 growth medium Substances 0.000 claims abstract description 19
- 230000000694 effects Effects 0.000 claims description 5
- 229910000831 Steel Inorganic materials 0.000 claims description 3
- 239000010959 steel Substances 0.000 claims description 3
- 238000001802 infusion Methods 0.000 claims 10
- 238000005086 pumping Methods 0.000 abstract description 24
- 238000009423 ventilation Methods 0.000 abstract description 9
- 230000008878 coupling Effects 0.000 abstract description 5
- 238000010168 coupling process Methods 0.000 abstract description 5
- 238000005859 coupling reaction Methods 0.000 abstract description 5
- 238000005273 aeration Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 18
- 210000004748 cultured cell Anatomy 0.000 description 14
- 239000002609 medium Substances 0.000 description 13
- 239000006260 foam Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 3
- 230000001464 adherent effect Effects 0.000 description 2
- 230000004103 aerobic respiration Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- C12M27/02—Stirrer or mobile mixing elements
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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Abstract
本发明涉及一种细胞灌注培养的生物反应器，包括培养罐、电机、旋转截留装置、空心轴、抽液管和通气管，空心轴与培养罐顶盖密封安装固定，旋转截留装置包括外转子、内转子、滤网和底板，外转子和内转子分别安装于培养罐顶盖的外部和内部，外转子通过磁力耦合作用带动内转子旋转，底板固定在空心轴的底部，底板与内转子通过滤网连接，滤网高出培养基液面，空心轴内设抽液腔，抽液腔与空心轴空腔隔绝、通过抽液孔与培养罐连通，抽液管设于空心轴内部、下端口位于抽液腔内，通气管与空心轴空腔连通，空心轴空腔侧壁设有通气孔。本发明实现在搅拌状态下能够同时完成抽出培养基和通气，不仅能够截留细胞，而且能够对气泡进行隔离并具有消泡的功能。
The invention relates to a bioreactor for cell perfusion culture, which includes a culture tank, a motor, a rotating retaining device, a hollow shaft, a liquid suction pipe and a ventilation pipe, the hollow shaft is sealed and fixed to the top cover of the cultivating tank, and the rotating retaining device includes an outer rotor , inner rotor, filter screen and bottom plate, the outer rotor and inner rotor are respectively installed on the outside and inside of the top cover of the culture tank, the outer rotor drives the inner rotor to rotate through magnetic coupling, the bottom plate is fixed on the bottom of the hollow shaft, and the bottom plate communicates with the inner rotor The filter screen is connected, the filter screen is higher than the liquid level of the culture medium, a pumping cavity is set inside the hollow shaft, the pumping cavity is isolated from the cavity of the hollow shaft, and communicates with the culture tank through the pumping hole, and the pumping tube is set inside the hollow shaft, the lower The port is located in the liquid pumping chamber, the vent pipe communicates with the cavity of the hollow shaft, and the side wall of the cavity of the hollow shaft is provided with a vent hole. The invention realizes that the pumping out of the culture medium and aeration can be completed simultaneously under the stirring state, not only the cells can be trapped, but also the air bubbles can be isolated and have the function of defoaming.
Description
技术领域technical field
本发明涉及一种生物反应器，特别是涉及一种细胞灌注培养的生物反应器。The invention relates to a bioreactor, in particular to a bioreactor for cell perfusion culture.
背景技术Background technique
在生物反应器内部对细胞进行有氧呼吸的悬浮灌注培养时，例如贴壁细胞的微载体培养，一方面需要实现部分培养基的抽出以及新鲜培养基的连续灌注，在这一过程中，需要通过细胞截留装置将培养细胞截留在培养罐内部而不会随着培养基被抽出，另一方面需要向培养基中通气保证有氧呼吸，在这一过程中，由于通气的作用使得培养基中产生气泡，这些气泡能够对培养细胞造成损害，而现有的生物反应器中一般不具有隔离气泡或者消泡的功能，影响了细胞培养的存活率，降低了细胞产物的产率。When performing aerobic respiration suspension perfusion culture on cells inside the bioreactor, such as microcarrier culture of adherent cells, on the one hand, it is necessary to realize the extraction of part of the medium and the continuous perfusion of fresh medium. In this process, it is necessary The cultured cells are retained inside the culture tank by the cell retaining device and will not be drawn out with the medium. On the other hand, it is necessary to ventilate the medium to ensure aerobic respiration. In this process, due to the effect of ventilation, the culture medium Bubbles are generated, and these bubbles can cause damage to the cultured cells, and the existing bioreactors generally do not have the function of isolating bubbles or defoaming, which affects the survival rate of cell culture and reduces the yield of cell products.
现有申请号为201120331457.2的实用新型专利，专利名称为“搅拌、通气及细胞过滤一体化生物反应器”，公开了一种能够在搅拌的同时实现抽液、通气和截留培养细胞的生物反应器，但是这种生物反应器采用了传统的搅拌轴结构，对于细胞培养存在微生物污染的风险，而且取液管和气体分布管在反应器顶盖上分别单独开口安装，进一步增加了污染风险。The existing application number is 201120331457.2 utility model patent, the patent name is "stirring, aeration and cell filtration integrated bioreactor", which discloses a bioreactor that can realize pumping, aeration and retention of cultured cells while stirring , but this bioreactor adopts the traditional stirring shaft structure, there is a risk of microbial contamination for cell culture, and the liquid pipe and gas distribution pipe are installed with separate openings on the top cover of the reactor, which further increases the risk of contamination.
发明内容Contents of the invention
本发明所要解决的技术问题是提供一种细胞灌注培养的生物反应器，不仅能够在搅拌状态下同时完成抽出培养基和通气，截留细胞，而且保证了无菌条件，还能够对气泡进行隔离并具有消泡的功能。The technical problem to be solved by the present invention is to provide a bioreactor for cell perfusion culture, which can not only complete the pumping out of the medium and aeration and retain the cells in a stirring state, but also ensure the aseptic conditions, and can also isolate the air bubbles. It has the function of defoaming.
本发明解决其技术问题所采用的技术方案是提供一种细胞灌注培养的生物反应器，包括培养罐、电机、旋转截留装置、空心轴、搅拌桨、抽液管和通气管，所述空心轴安装在培养罐的顶盖中心，所述搅拌桨位于空心轴的底部位置，所述旋转截留装置通过电机驱动旋转，所述空心轴与培养罐顶盖密封安装并固定，所述旋转截留装置包括外转子、内转子、滤网和底板，所述外转子和内转子相对配合安装于培养罐顶盖的外部和内部，所述外转子和内转子相互配合、通过空心轴分别安装于培养罐顶盖的外部和内部，所述外转子通过电机驱动旋转并通过磁力耦合作用带动内转子沿着空心轴旋转，所述底板可旋转式固定在空心轴的底部，底板与内转子之间通过滤网连接形成培养细胞的截留界面，所述滤网高出培养基的液面，所述空心轴内部设有抽液腔，所述抽液腔与空心轴的空腔隔绝、通过若干抽液孔与培养罐连通，所述抽液管设置于空心轴内部、下端口位于抽液腔内部，所述通气管与空心轴的空腔连通，所述空心轴的空腔侧壁设有若干通气孔。The technical solution adopted by the present invention to solve the technical problem is to provide a bioreactor for perfusion culture of cells, including a culture tank, a motor, a rotating retaining device, a hollow shaft, a stirring paddle, a suction pipe and an air pipe, and the hollow shaft Installed in the center of the top cover of the culture tank, the stirring paddle is located at the bottom of the hollow shaft, the rotating retaining device is driven to rotate by a motor, the hollow shaft is sealed and fixed to the top cover of the cultivating tank, and the rotating retaining device includes The outer rotor, the inner rotor, the filter screen and the bottom plate, the outer rotor and the inner rotor are relatively matched and installed on the outside and the inside of the top cover of the culture tank, and the outer rotor and the inner rotor cooperate with each other and are respectively installed on the top of the culture tank through a hollow shaft The outside and inside of the cover, the outer rotor is driven to rotate by the motor and drives the inner rotor to rotate along the hollow shaft through magnetic coupling, the bottom plate is rotatably fixed at the bottom of the hollow shaft, and the filter screen is passed between the bottom plate and the inner rotor connected to form a retaining interface for cultured cells, the filter screen is higher than the liquid level of the culture medium, and the hollow shaft is provided with a pumping cavity inside, and the pumping cavity is isolated from the cavity of the hollow shaft, and connected to the cavity through several pumping holes. The culture tank is connected, the pumping tube is arranged inside the hollow shaft, the lower port is located inside the pumping cavity, the vent tube is connected to the cavity of the hollow shaft, and the cavity side wall of the hollow shaft is provided with a number of vent holes.
作为本发明一种优选的实施方式，所述外转子和内转子内部、相对侧面对应设有磁钢。As a preferred embodiment of the present invention, the outer rotor and the inner rotor are respectively provided with magnetic steels on opposite sides.
作为本发明另一种优选的实施方式，所述搅拌桨安装在底板上方并随着旋转截留装置转动。As another preferred embodiment of the present invention, the stirring paddle is installed above the bottom plate and rotates with the rotating retaining device.
作为本发明另一种优选的实施方式，所述滤网的孔径小于培养基中内容物的大小。As another preferred embodiment of the present invention, the pore size of the filter is smaller than the content in the culture medium.
作为本发明另一种优选的实施方式，所述底板的大小与内转子的底面相等，所述滤网竖直设置。As another preferred embodiment of the present invention, the size of the bottom plate is equal to the bottom surface of the inner rotor, and the filter screen is arranged vertically.
作为本发明另一种优选的实施方式，所述抽液腔设置于空心轴的底部，所述抽液管下端口伸入到抽液腔内部，抽液腔与空心轴的空腔之间通过水平隔板隔绝。As another preferred embodiment of the present invention, the liquid suction chamber is arranged at the bottom of the hollow shaft, the lower port of the liquid suction tube extends into the liquid suction chamber, and the liquid suction chamber and the cavity of the hollow shaft pass through Horizontal divider isolated.
作为对上述实施方式的进一步改进，所述若干抽液孔沿着抽液腔的侧壁周向均匀分布。As a further improvement to the above embodiment, the plurality of liquid suction holes are evenly distributed along the circumference of the side wall of the liquid suction cavity.
作为对上述实施方式的进一步改进，所述若干通气孔设置于靠近抽液腔的上方、沿着空心轴空腔侧壁的周向均匀分布。As a further improvement to the above-mentioned embodiment, the plurality of vent holes are disposed close to the upper part of the liquid suction chamber and evenly distributed along the circumference of the side wall of the cavity of the hollow shaft.
作为本发明另一种优选的实施方式，所述抽液管从空心轴的顶端穿出。As another preferred embodiment of the present invention, the liquid suction tube passes through the top end of the hollow shaft.
作为本发明另一种优选的实施方式，所述通气管与空心轴的顶端连通。As another preferred embodiment of the present invention, the ventilation pipe communicates with the top end of the hollow shaft.
有益效果Beneficial effect
本发明的旋转截留装置采用磁力耦合的方式进行驱动并沿着空心轴旋转，带动搅拌桨对培养基进行搅拌，内转子、滤网以及底板之间形成了相对的内部空间，空心轴位于该内部空间之中，空心轴与培养罐密封固定不会发生旋转，保证了密封无菌条件；抽液管设置于空心轴内部进行培养基抽出，通气管通过空心轴的空腔进行通气，通气与培养基抽出过程相互独立，实现了在旋转截留装置旋转搅拌的状态下能够同时进行培养基抽出和通气；内转子与底板之间设有用于截留培养细胞的滤网，既可以防止外部培养基中的细胞进入到旋转截留装置的内部被抽出，又能够防止旋转截留装置内部产生的气泡与外部的培养细胞接触，从而实现气泡隔离的效果，减少气泡对于培养细胞的损伤，增加细胞存活率；通气管通入的气体从旋转截留装置内部进入培养罐，滤网的设置高出于培养基的液面，这种结构设计使得内转子底面、滤网以及培养基液面之间形成了一个相对封闭的包围，气泡进入到旋转截留装置的内部空间后会上升，并在培养基液面上产生聚集形成泡沫，当泡沫在该封闭的包围内达到饱和后，该封闭的包围能够对泡沫产生挤压作用，从而达到消泡的功能。The rotary retaining device of the present invention is driven by magnetic coupling and rotates along the hollow shaft to drive the stirring paddle to stir the culture medium. A relative inner space is formed between the inner rotor, the filter screen and the bottom plate, and the hollow shaft is located in the inner space. In the space, the hollow shaft and the culture tank are sealed and fixed without rotation, ensuring a sealed and sterile condition; the suction tube is set inside the hollow shaft to extract the culture medium, and the ventilation tube is ventilated through the cavity of the hollow shaft. The process of extracting the substrates is independent of each other, so that the medium can be extracted and ventilated at the same time in the state of rotation and stirring of the rotary retaining device; there is a filter screen between the inner rotor and the bottom plate for retaining the cultured cells, which can prevent the external culture medium. The cells enter the interior of the rotary retaining device and are drawn out, which can also prevent the bubbles generated inside the rotary retaining device from contacting the cultured cells outside, so as to achieve the effect of bubble isolation, reduce the damage of the bubbles to the cultured cells, and increase the cell survival rate; the ventilation tube The fed gas enters the culture tank from the inside of the rotary trapping device, and the filter screen is set higher than the liquid level of the medium. This structural design makes a relatively closed gap between the bottom surface of the inner rotor, the filter screen and the liquid level of the medium. Surrounding, the bubbles will rise after entering the internal space of the rotary trapping device, and gather on the surface of the culture medium to form foam. When the foam reaches saturation in the closed enclosure, the closed enclosure can squeeze the foam , so as to achieve the function of defoaming.
附图说明Description of drawings
图1为本发明的结构示意图。Fig. 1 is a structural schematic diagram of the present invention.
具体实施方式detailed description
下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。此外应理解，在阅读了本发明讲授的内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.
如图1所示的一种细胞灌注培养的生物反应器，包括培养罐12、电机1、旋转截留装置、空心轴4、搅拌桨15、抽液管2和通气管3。A bioreactor for perfusion culture of cells as shown in FIG.
空心轴4为内部空心的圆柱状结构，与培养罐12顶盖在其中心密封安装并固定，空心轴4设置成固定方式，不会发生旋转。The hollow shaft 4 is an inner hollow cylindrical structure, which is sealed and fixed with the top cover of the culture tank 12 at its center, and the hollow shaft 4 is arranged in a fixed manner so as not to rotate.
旋转截留装置包括外转子5、内转子6、滤网7和底板10。外转子5和内转子6相对配合安装于培养罐12顶盖的外部和内部，外转子5通过电机1驱动沿着空心轴4发生旋转，并通过磁力耦合作用带动内转子6沿着空心轴4旋转，外转子5和内转子6内部、相对侧面对应设有磁钢11。The rotary interceptor includes an outer rotor 5 , an inner rotor 6 , a screen 7 and a bottom plate 10 . The outer rotor 5 and the inner rotor 6 are relatively matched and installed on the outside and inside of the top cover of the culture tank 12. The outer rotor 5 is driven by the motor 1 to rotate along the hollow shaft 4, and drives the inner rotor 6 along the hollow shaft 4 through magnetic coupling. Rotating, the outer rotor 5 and the inner rotor 6 are provided with magnetic steel 11 correspondingly on the opposite sides.
底板10可旋转地固定在空心轴4的底部，底板10的大小与内转子6的底面相等，搅拌桨15固定安装在底板10上方并随着旋转截留装置转动，对培养罐12内部的培养基13进行搅拌。底板10与内转子6之间通过滤网7连接形成培养细胞的截留界面，滤网7为竖直的圆筒状，滤网7的孔径小于培养基13中内容物的大小，例如在自悬浮细胞的培育中，滤网7的孔径选择要小于细胞的大小，在贴壁细胞的微载体培养中，滤网7的孔径小于微载体的大小，保证培养细胞不能从其孔径中穿过，滤网7高出培养基13的液面。在培养罐12内部，内转子6、滤网7以及底板10之间形成了一个相对的内部空间，通过滤网7的作用，培养细胞不能进入到该内部空间中，搅拌桨15设置于该空间内部，能够有效防止在搅拌过程中搅拌桨15对培养细胞造成机械损伤。The base plate 10 is rotatably fixed on the bottom of the hollow shaft 4, the size of the base plate 10 is equal to the bottom surface of the inner rotor 6, and the stirring paddle 15 is fixedly installed above the base plate 10 and rotates with the rotating retaining device, and the culture medium inside the culture tank 12 13 for stirring. The bottom plate 10 and the inner rotor 6 are connected by a filter screen 7 to form a retaining interface for culturing cells. The filter screen 7 is a vertical cylinder, and the aperture of the filter screen 7 is smaller than the size of the content in the medium 13, such as in self-suspension In the cultivation of cells, the aperture of the filter screen 7 is selected to be smaller than the size of the cells. In the microcarrier culture of adherent cells, the aperture of the filter screen 7 is smaller than the size of the microcarrier to ensure that the cultured cells cannot pass through the aperture. The net 7 is above the liquid level of the culture medium 13 . Inside the culture tank 12, a relative internal space is formed between the inner rotor 6, the filter screen 7 and the bottom plate 10. Through the effect of the filter screen 7, the cultured cells cannot enter the internal space, and the stirring paddle 15 is arranged in this space. Inside, it can effectively prevent the mechanical damage caused by the stirring paddle 15 to the cultured cells during the stirring process.
空心轴4内部设有抽液腔14，抽液腔14与空心轴4的空腔隔绝，抽液腔14通过若干抽液孔9与培养罐12连通，抽液管2设置于空心轴4内部、下端口位于抽液腔14内，抽液管2从抽液腔14内部向培养罐12外抽出培养基13。通气管3与空心轴4的空腔连通，空心轴4的空腔侧壁设有若干通气孔8，通气管3通过空心轴4空腔向培养基13中通气。The hollow shaft 4 is provided with a pumping chamber 14, which is isolated from the cavity of the hollow shaft 4. The pumping chamber 14 communicates with the culture tank 12 through a number of pumping holes 9, and the pumping tube 2 is arranged inside the hollow shaft 4. , The lower port is located in the liquid suction chamber 14, and the liquid suction pipe 2 draws out the culture medium 13 from the inside of the liquid suction chamber 14 to the outside of the culture tank 12. The ventilation pipe 3 communicates with the cavity of the hollow shaft 4 , and the cavity side wall of the hollow shaft 4 is provided with a plurality of ventilation holes 8 , and the ventilation pipe 3 ventilates into the culture medium 13 through the cavity of the hollow shaft 4 .
关于空心轴4，一种优选的结构为：抽液腔14设置于空心轴4的底部，抽液管2下端口伸入到抽液腔14内部，抽液腔14与空心轴4的空腔之间通过水平隔板隔绝，防止抽液腔14中的培养基13进入到空心轴4的空腔，若干抽液孔9沿着抽液腔14的侧壁周向均匀分布，若干通气孔8设置于靠近抽液腔14的上方，沿着空心轴4空腔侧壁的周向均匀分布，抽液管2从空心轴4的顶端穿出，通气管3与空心轴4的顶端连通。Regarding the hollow shaft 4, a preferred structure is: the pumping cavity 14 is arranged at the bottom of the hollow shaft 4, the lower port of the pumping tube 2 extends into the liquid pumping cavity 14, and the cavity of the pumping cavity 14 and the hollow shaft 4 They are isolated by horizontal partitions to prevent the culture medium 13 in the pumping cavity 14 from entering the cavity of the hollow shaft 4. Several pumping holes 9 are evenly distributed along the circumference of the side wall of the pumping cavity 14. Several vent holes 8 It is arranged above the suction chamber 14 and evenly distributed along the circumference of the side wall of the hollow shaft 4 .
这种细胞灌注培养的生物反应器的旋转截留装置采用磁力耦合的方式进行驱动并沿着空心轴4旋转，对培养基13进行搅拌，空心轴4位于旋转截留装置形成的内部空间之中，空心轴4与培养罐12密封固定不会发生旋转，不仅保证了无菌条件，抽液管2设置于空心轴4内部进行培养基13抽出，通气管3通过空心轴4的空腔进行通气，通气与培养基13抽出过程相互独立，实现了在旋转截留装置旋转搅拌的状态下能够同时进行培养基13抽出和通气。The rotary retaining device of this cell perfusion culture bioreactor is driven by magnetic coupling and rotates along the hollow shaft 4 to stir the medium 13. The hollow shaft 4 is located in the inner space formed by the rotating retaining device. The shaft 4 and the culture tank 12 are sealed and fixed without rotation, which not only ensures aseptic conditions, but also the suction tube 2 is set inside the hollow shaft 4 to extract the culture medium 13, and the ventilation tube 3 is ventilated through the cavity of the hollow shaft 4. The extraction process of the medium 13 is independent of each other, so that the medium 13 can be extracted and ventilated at the same time in the state of rotation and stirring of the rotary retaining device.
内转子6与底板10之间设有用于截留培养细胞的滤网7，既可以防止外部培养基13中的培养细胞进入到旋转截留装置的内部被抽出，又能够防止旋转截留装置内部产生的气泡与外部的培养细胞接触，从而到达气泡隔离的效果，减少气泡对于培养细胞的损伤，增加细胞存活率。Between the inner rotor 6 and the bottom plate 10 is provided a filter screen 7 for intercepting the cultured cells, which can prevent the cultured cells in the external culture medium 13 from entering the interior of the rotary interception device and being drawn out, and can also prevent air bubbles generated inside the rotary interception device. Contact with the external cultured cells, so as to achieve the effect of air bubble isolation, reduce the damage of air bubbles to the cultured cells, and increase the cell survival rate.
通气管3通入的气体从旋转截留装置内部进入培养罐12，滤网7的设置高出于培养基13的液面，这种结构设计使得内转子6底面、滤网7以及培养基13液面之间形成了一个相对封闭的包围，气泡进入到旋转截留装置的内部空间后会上升，并在培养基13液面上产生聚集形成泡沫，当泡沫在该封闭的包围内达到饱和后，该封闭的包围能够对泡沫产生挤压作用，从而达到消泡的功能。The gas introduced by the vent pipe 3 enters the culture tank 12 from the inside of the rotary intercepting device, and the filter screen 7 is set higher than the liquid level of the medium 13. This structural design makes the bottom surface of the inner rotor 6, the filter screen 7 and the medium 13 liquid A relatively closed enclosure is formed between the surfaces, and the air bubbles will rise after entering the internal space of the rotary trapping device, and gather on the liquid surface of the culture medium 13 to form foam. When the foam reaches saturation in the closed enclosure, the The closed enclosure can squeeze the foam so as to achieve the function of defoaming.
Claims (10)
- null1. the bioreactor that a Cell infusion is cultivated，Including culture tank (12)、Motor (1)、Rotate device for trapping、Hollow axle (4)、Stirring paddle (15)、Liquid suction pipe (2) and breather (3)，Described hollow axle (4) is arranged on the top cap central of culture tank (12)，Described stirring paddle (15) is positioned at the bottom position of hollow axle (4)，Described rotation device for trapping is driven by motor (1) and rotates，It is characterized in that: described hollow axle (4) installs and fixes with culture tank (12) top seal，Described rotation device for trapping includes outer rotor (5)、Internal rotor (6)、Filter screen (7) and base plate (10)，Described outer rotor (5) and internal rotor (6) relative engagement are installed on culture tank (12) top cover outwardly and inwardly，Described outer rotor (5) is driven by motor (1) and rotates and drive internal rotor (6) to rotate along hollow axle (4) by magnetic couple effect，Described base plate (10) rotary type is fixed on the bottom of hollow axle (4)，Between base plate (10) with internal rotor (6) by filter screen (7) is connected formed cultivation cell retain interface，Described filter screen (7) exceeds the liquid level of culture medium (13)，Described hollow axle (4) is internal is provided with drawing liquid chamber (14)，Described drawing liquid chamber (14) completely cuts off with the cavity of hollow axle (4)、Connected with culture tank (12) by some drawing liquid holes (9)，It is internal that described liquid suction pipe (2) is arranged at hollow axle (4)、It is internal that lower port is positioned at drawing liquid chamber (14)，Described breather (3) connects with the cavity of hollow axle (4)，The cavity sidewalls of described hollow axle (4) is provided with some passages (8).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described outer rotor (5) and internal rotor (6) inside, opposite flank are correspondingly provided with magnet steel (11).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described stirring paddle (15) is arranged on base plate (10) top and rotates along with rotating device for trapping.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: the aperture of described filter screen (7) is less than the size of content in culture medium (13).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: the size of described base plate (10) is equal with the bottom surface of internal rotor (6), and described filter screen (7) is vertically arranged.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterized in that: described drawing liquid chamber (14) is arranged at the bottom of hollow axle (4), it is internal that described liquid suction pipe (2) lower port extend into drawing liquid chamber (14), is completely cut off by horizontal baffle between the cavity of drawing liquid chamber (14) and hollow axle (4).
- The bioreactor that a kind of Cell infusion the most according to claim 6 is cultivated, it is characterised in that: described some drawing liquid holes (9) are uniformly distributed along the sidewall circumference of drawing liquid chamber (14).
- The bioreactor that a kind of Cell infusion the most according to claim 6 is cultivated, it is characterised in that: described some passages (8) are disposed in proximity to the top of drawing liquid chamber (14), are uniformly distributed along the circumference of hollow axle (4) cavity sidewalls.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described liquid suction pipe (2) passes from the top of hollow axle (4).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described breather (3) connects with the top of hollow axle (4).
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