CN105820952A - Bioreactor for cell perfusion culture - Google Patents
Bioreactor for cell perfusion culture Download PDFInfo
- Publication number
- CN105820952A CN105820952A CN201610197422.1A CN201610197422A CN105820952A CN 105820952 A CN105820952 A CN 105820952A CN 201610197422 A CN201610197422 A CN 201610197422A CN 105820952 A CN105820952 A CN 105820952A
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- China
- Prior art keywords
- hollow axle
- bioreactor
- cultivated
- drawing liquid
- filter screen
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention relates to a bioreactor for cell perfusion culture.The bioreactor for cell perfusion culture comprises a culture tank, a motor, a rotary interception device, a hollow shaft, a liquid suction pipe and a breather pipe, wherein the hollow shaft is installed and fixed with a top cover of the culture tank in a sealed, the rotary interception device comprises an outer rotor, an inner rotor, a filter screen and a base plate, the outer rotor and the inner rotor are respectively installed outside and inside the top cover of the culture tank, the outer rotor drives the inner rotor to rotate through a magnetic coupling effect, the base plate is fixed to the bottom of the hollow shaft, the base plate is connected with the inner rotor through the filter screen, the filter screen is higher than the liquid level of a culture medium, a liquid suction cavity is formed in the hollow shaft, is isolated from the cavity of the hollow shaft and is communicated with the culture tank through liquid suction holes, the liquid suction pipe is arranged inside the hollow shaft, a lower port of the liquid suction pipe is located in the liquid suction cavity, the breather pipe is communicated with the cavity of the hollow shaft, and breather holes are formed in the side wall of the cavity of the hollow shaft.The bioreactor for cell perfusion culture can simultaneously achieve culture medium suction and ventilation in a stirring state, can intercept cells, can also isolate air bubbles and has a bubble eliminating function.
Description
Technical field
The present invention relates to a kind of bioreactor, particularly relate to the bioreactor that a kind of Cell infusion is cultivated.
Background technology
When cell is carried out inside bioreactor the suspension perfusion cultures of aerobic respiration, the microcarrier of such as attached cell is cultivated, on the one hand need to realize the continuous pouring of the extraction of partial medium and fresh culture, in this course, need by cell retention device by cultivation cell retention inside culture tank without being drawn out of along with culture medium, on the other hand ventilation in culture medium is needed to ensure aerobic respiration, in this course, owing to the effect of ventilation makes to produce in culture medium bubble, these bubbles can cause damage to cultivating cell, and existing bioreactor does not typically have the function of separation bubble or froth breaking, have impact on the survival rate that cell is cultivated, reduce the productivity of cellular products.
The utility model patent of existing Application No. 201120331457.2, patent name is " stir, ventilate and cell filtration integrated bioreactor ", disclose a kind of can stirring while realize drawing liquid, ventilate and retain cultivate cell bioreactor, but this bioreactor have employed traditional shaft structure, cell is cultivated and there is the risk that microorganism is polluted, and the individually opening installation on reactor top lid of liquid flowing tube and gas distribution tube, further increase pollution risk.
Summary of the invention
The technical problem to be solved is to provide the bioreactor that a kind of Cell infusion is cultivated, can not only complete to extract culture medium and ventilation out under stirring simultaneously, entrapped cell, and ensure that aseptic condition, additionally it is possible to bubble is isolated and is had the function of froth breaking.
nullThe technical solution adopted for the present invention to solve the technical problems is to provide the bioreactor that a kind of Cell infusion is cultivated，Including culture tank、Motor、Rotate device for trapping、Hollow axle、Stirring paddle、Liquid suction pipe and breather，Described hollow axle is arranged on the top cap central of culture tank，Described stirring paddle is positioned at the bottom position of hollow axle，Described rotation device for trapping is driven by motor and rotates，Described hollow axle installs and fixes with culture tank top seal，Described rotation device for trapping includes outer rotor、Internal rotor、Filter screen and base plate，Described outer rotor and internal rotor relative engagement are installed on culture tank top cover outwardly and inwardly，Described outer rotor and internal rotor cooperate、It is respectively arranged in culture tank top cover outwardly and inwardly by hollow axle，Described outer rotor is driven by motor and rotates and drive internal rotor to rotate along hollow axle by magnetic couple effect，Described base plate rotary type is fixed on the bottom of hollow axle，Between base plate with internal rotor by filter screen be connected formed cultivate cell retain interface，Described filter screen exceeds the liquid level of culture medium，Drawing liquid chamber it is provided with inside described hollow axle，Described drawing liquid chamber completely cuts off with the cavity of hollow axle、Connected with culture tank by some drawing liquid holes，Described liquid suction pipe is arranged at inside hollow axle、Lower port is positioned at drawing liquid intracavity portion，Described breather connects with the cavity of hollow axle，The cavity sidewalls of described hollow axle is provided with some passages.
As one of the present invention preferred embodiment, inside described outer rotor and internal rotor, opposite flank is correspondingly provided with magnet steel.
As another preferred embodiment of the present invention, described stirring paddle is arranged on above base plate and rotates along with rotating device for trapping.
As another preferred embodiment of the present invention, the aperture of described filter screen is less than the size of content in culture medium.
As another preferred embodiment of the present invention, the size of described base plate is equal with the bottom surface of internal rotor, and described filter screen is vertically arranged.
As another preferred embodiment of the present invention, described drawing liquid chamber is arranged at the bottom of hollow axle, and described liquid suction pipe lower port is extend into drawing liquid intracavity portion, completely cut off by horizontal baffle between drawing liquid chamber and the cavity of hollow axle.
As the further improvement to above-mentioned embodiment, described some drawing liquid holes are uniformly distributed along the sidewall circumference in drawing liquid chamber.
As the further improvement to above-mentioned embodiment, described some passages are disposed in proximity to the top in drawing liquid chamber, are uniformly distributed along the circumference of hollow axle cavity sidewalls.
As another preferred embodiment of the present invention, described liquid suction pipe passes from the top of hollow axle.
As another preferred embodiment of the present invention, described breather connects with the top of hollow axle.
Beneficial effect
The device for trapping that rotates of the present invention uses the mode of magnetic couple to be driven and rotates along hollow axle, drive stirring paddle that culture medium is stirred, relative inner space is defined between internal rotor, filter screen and base plate, hollow axle is positioned among this inner space, hollow axle and culture tank seal fixes and will not rotate, it is ensured that sealed, sterile condition；Liquid suction pipe is arranged at inside hollow axle and carries out culture medium extraction, breather is ventilated by the cavity of hollow axle, ventilate separate with culture medium extraction, it is achieved that culture medium extraction and ventilation can be carried out when rotating device for trapping Stirring simultaneously；It is provided with for retaining the filter screen cultivating cell between internal rotor and base plate, both the inside being possible to prevent the cell in External Medium to enter into rotation device for trapping is drawn out of, it is prevented from again rotating the internal bubble produced of device for trapping and outside cultivation cells contacting, thus realize the effect of bubble isolation, reduce the bubble damage for cultivation cell, increase cell survival rate；From rotating, device for trapping is internal enters culture tank to the gas that breather is passed through, the setting of filter screen is above the liquid level of culture medium, the design of this structure makes to define between internal rotor bottom surface, filter screen and culture medium liquid level the encirclement of a relative closure, bubble can rise after entering into the inner space rotating device for trapping, and on culture medium liquid level, produce gathering formation foam, when foam reach in the encirclement of this closing saturated after, the encirclement of this closing can produce squeezing action to foam, thus reaches the function of froth breaking.
Accompanying drawing explanation
Fig. 1 is the structural representation of the present invention.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is expanded on further.Should be understood that these embodiments are merely to illustrate the present invention rather than limit the scope of the present invention.In addition, it is to be understood that after having read the content that the present invention lectures, the present invention can be made various changes or modifications by those skilled in the art, and these equivalent form of values fall within the application appended claims limited range equally.
The bioreactor that a kind of Cell infusion as shown in Figure 1 is cultivated, including culture tank 12, motor 1, rotates device for trapping, hollow axle 4, stirring paddle 15, liquid suction pipe 2 and breather 3.
Hollow axle 4 is the cylindrical-shaped structure of boring, seals with the culture tank 12 top cover heart wherein and installs and fixes, and hollow axle 4 is arranged to fixed form, will not rotate.
Rotate device for trapping and include outer rotor 5, internal rotor 6, filter screen 7 and base plate 10.Outer rotor 5 and internal rotor 6 relative engagement are installed on culture tank 12 top cover outwardly and inwardly, outer rotor 5 is driven by motor 1 and rotates along hollow axle 4, and driving internal rotor 6 to rotate along hollow axle 4 by magnetic couple effect, outer rotor 5 and internal rotor 6 inside, opposite flank are correspondingly provided with magnet steel 11.
Base plate 10 is removably secured to the bottom of hollow axle 4, and the size of base plate 10 is equal with the bottom surface of internal rotor 6, and stirring paddle 15 is fixedly mounted on above base plate 10 and rotates along with rotating device for trapping, is stirred the culture medium 13 within culture tank 12.Between base plate 10 with internal rotor 6 by filter screen 7 is connected formed cultivation cell retain interface, filter screen 7 is vertical cylindrical shape, the aperture of filter screen 7 is less than the size of content in culture medium 13, such as in the cultivation of suspension cell, the aperture of filter screen 7 selects to be less than the size of cell, and in the microcarrier of attached cell is cultivated, the aperture of filter screen 7 is less than the size of microcarrier, ensureing that cultivating cell can not pass from its aperture, filter screen 7 exceeds the liquid level of culture medium 13.Inside culture tank 12, a relative inner space is defined between internal rotor 6, filter screen 7 and base plate 10, effect by filter screen 7, cultivate cell and can not enter in this inner space, stirring paddle 15 is arranged at this interior volume, it is possible to effectively prevent stirring paddle 15 in whipping process from causing mechanical damage to cultivating cell.
Hollow axle 4 is internal is provided with drawing liquid chamber 14, drawing liquid chamber 14 completely cuts off with the cavity of hollow axle 4, drawing liquid chamber 14 is connected with culture tank 12 by some drawing liquid holes 9, liquid suction pipe 2 is arranged at hollow axle 4 inside, lower port is positioned at drawing liquid chamber 14, and liquid suction pipe 2 is internal from drawing liquid chamber 14 extracts culture medium 13 out to culture tank 12.Breather 3 connects with the cavity of hollow axle 4, and the cavity sidewalls of hollow axle 4 is provided with some passages 8, and breather 3 is ventilated in culture medium 13 by hollow axle 4 cavity.
About hollow axle 4, a kind of preferably structure is: drawing liquid chamber 14 is arranged at the bottom of hollow axle 4, it is internal that liquid suction pipe 2 lower port extend into drawing liquid chamber 14, completely cut off by horizontal baffle between the cavity of drawing liquid chamber 14 and hollow axle 4, prevent the culture medium 13 in drawing liquid chamber 14 from entering into the cavity of hollow axle 4, some drawing liquid holes 9 are uniformly distributed along the sidewall circumference in drawing liquid chamber 14, some passages 8 are disposed in proximity to the top in drawing liquid chamber 14, circumference along hollow axle 4 cavity sidewalls is uniformly distributed, liquid suction pipe 2 passes from the top of hollow axle 4, breather 3 connects with the top of hollow axle 4.
The device for trapping that rotates of the bioreactor that this Cell infusion is cultivated uses the mode of magnetic couple be driven and rotate along hollow axle 4, culture medium 13 is stirred, hollow axle 4 is positioned at and rotates among the inner space that device for trapping is formed, hollow axle 4 and culture tank 12 seal fixes and will not rotate, not only ensure that aseptic condition, liquid suction pipe 2 is arranged at that hollow axle 4 is internal to carry out culture medium 13 and extract out, breather 3 is ventilated by the cavity of hollow axle 4, ventilate separate with culture medium 13 extraction, achieve and can carry out culture medium 13 simultaneously extract out when rotating device for trapping Stirring and ventilate.
It is provided with for retaining the filter screen 7 cultivating cell between internal rotor 6 and base plate 10, both the cultivation cell being possible to prevent in External Medium 13 enters into the inside of rotation device for trapping and is drawn out of, it is prevented from again rotating the internal bubble produced of device for trapping and outside cultivation cells contacting, thus arrive the effect of bubble isolation, reduce the bubble damage for cultivation cell, increase cell survival rate.
From rotating, device for trapping is internal enters culture tank 12 to the gas that breather 3 is passed through, the setting of filter screen 7 is above the liquid level of culture medium 13, the design of this structure makes to define between internal rotor 6 bottom surface, filter screen 7 and culture medium 13 liquid level the encirclement of a relative closure, bubble can rise after entering into the inner space rotating device for trapping, and on culture medium 13 liquid level, produce gathering formation foam, when foam reach in the encirclement of this closing saturated after, the encirclement of this closing can produce squeezing action to foam, thus reaches the function of froth breaking.
Claims (10)
- null1. the bioreactor that a Cell infusion is cultivated，Including culture tank (12)、Motor (1)、Rotate device for trapping、Hollow axle (4)、Stirring paddle (15)、Liquid suction pipe (2) and breather (3)，Described hollow axle (4) is arranged on the top cap central of culture tank (12)，Described stirring paddle (15) is positioned at the bottom position of hollow axle (4)，Described rotation device for trapping is driven by motor (1) and rotates，It is characterized in that: described hollow axle (4) installs and fixes with culture tank (12) top seal，Described rotation device for trapping includes outer rotor (5)、Internal rotor (6)、Filter screen (7) and base plate (10)，Described outer rotor (5) and internal rotor (6) relative engagement are installed on culture tank (12) top cover outwardly and inwardly，Described outer rotor (5) is driven by motor (1) and rotates and drive internal rotor (6) to rotate along hollow axle (4) by magnetic couple effect，Described base plate (10) rotary type is fixed on the bottom of hollow axle (4)，Between base plate (10) with internal rotor (6) by filter screen (7) is connected formed cultivation cell retain interface，Described filter screen (7) exceeds the liquid level of culture medium (13)，Described hollow axle (4) is internal is provided with drawing liquid chamber (14)，Described drawing liquid chamber (14) completely cuts off with the cavity of hollow axle (4)、Connected with culture tank (12) by some drawing liquid holes (9)，It is internal that described liquid suction pipe (2) is arranged at hollow axle (4)、It is internal that lower port is positioned at drawing liquid chamber (14)，Described breather (3) connects with the cavity of hollow axle (4)，The cavity sidewalls of described hollow axle (4) is provided with some passages (8).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described outer rotor (5) and internal rotor (6) inside, opposite flank are correspondingly provided with magnet steel (11).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described stirring paddle (15) is arranged on base plate (10) top and rotates along with rotating device for trapping.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: the aperture of described filter screen (7) is less than the size of content in culture medium (13).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: the size of described base plate (10) is equal with the bottom surface of internal rotor (6), and described filter screen (7) is vertically arranged.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterized in that: described drawing liquid chamber (14) is arranged at the bottom of hollow axle (4), it is internal that described liquid suction pipe (2) lower port extend into drawing liquid chamber (14), is completely cut off by horizontal baffle between the cavity of drawing liquid chamber (14) and hollow axle (4).
- The bioreactor that a kind of Cell infusion the most according to claim 6 is cultivated, it is characterised in that: described some drawing liquid holes (9) are uniformly distributed along the sidewall circumference of drawing liquid chamber (14).
- The bioreactor that a kind of Cell infusion the most according to claim 6 is cultivated, it is characterised in that: described some passages (8) are disposed in proximity to the top of drawing liquid chamber (14), are uniformly distributed along the circumference of hollow axle (4) cavity sidewalls.
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described liquid suction pipe (2) passes from the top of hollow axle (4).
- The bioreactor that a kind of Cell infusion the most according to claim 1 is cultivated, it is characterised in that: described breather (3) connects with the top of hollow axle (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610197422.1A CN105820952A (en) | 2016-03-31 | 2016-03-31 | Bioreactor for cell perfusion culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610197422.1A CN105820952A (en) | 2016-03-31 | 2016-03-31 | Bioreactor for cell perfusion culture |
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| Publication Number | Publication Date |
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| CN105820952A true CN105820952A (en) | 2016-08-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610197422.1A Pending CN105820952A (en) | 2016-03-31 | 2016-03-31 | Bioreactor for cell perfusion culture |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018048181A3 (en) * | 2016-09-06 | 2018-05-03 | 한국화학연구원 | Cell culture receptacle |
| CN111004713A (en) * | 2019-12-27 | 2020-04-14 | 华中科技大学鄂州工业技术研究院 | Culture apparatus for microorganism dynamic growth environment |
| US20210238530A1 (en) * | 2020-02-05 | 2021-08-05 | Mott Corporation | Bioreactor design and methods of manufacture thereof |
| CN116676192A (en) * | 2023-06-07 | 2023-09-01 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018048181A3 (en) * | 2016-09-06 | 2018-05-03 | 한국화학연구원 | Cell culture receptacle |
| CN111004713A (en) * | 2019-12-27 | 2020-04-14 | 华中科技大学鄂州工业技术研究院 | Culture apparatus for microorganism dynamic growth environment |
| US20210238530A1 (en) * | 2020-02-05 | 2021-08-05 | Mott Corporation | Bioreactor design and methods of manufacture thereof |
| CN116676192A (en) * | 2023-06-07 | 2023-09-01 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
| CN116676192B (en) * | 2023-06-07 | 2023-11-14 | 上海多宁生物科技股份有限公司 | Bioreactor convenient for extracting and supplementing materials and used for CHO cell culture and method thereof |
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