CN102604830A - Device for operating and culturing animal embryo in vitro - Google Patents
Device for operating and culturing animal embryo in vitro Download PDFInfo
- Publication number
- CN102604830A CN102604830A CN2011104570585A CN201110457058A CN102604830A CN 102604830 A CN102604830 A CN 102604830A CN 2011104570585 A CN2011104570585 A CN 2011104570585A CN 201110457058 A CN201110457058 A CN 201110457058A CN 102604830 A CN102604830 A CN 102604830A
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- embryo
- vitro
- operation cell
- petridish
- operating
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/06—Bioreactors or fermenters specially adapted for specific uses for in vitro fertilization
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a device for operating and culturing an animal embryo in vitro, which can be used for rapidly and more rapidly and conveniently carrying out embryo washing in embryo operation, so that the embryo developmental rate is improved. The device disclosed by the invention comprises a culture dish and a culture dish cover, wherein the culture dish comprises small operation chambers for embryo in-vitro operation in vitro. The culture dish cover comprises a plurality of small operation chamber covers capable of being independently turned over. Each small operation chamber cover is used for covering a row of small operation chambers. According to the device for operating and culturing the animal embryo in vitro, the defects in the conventional embryo operation in vitro of time waste and inconvenience can be overcome. The embryo is washed in a process of freely falling down from the upper part of operation liquid to the bottom of the operation liquid without carrying out excessively blowing and beating on the embryo, so that the embryo is relatively easy to absorb. The water volatilization of the other rows of non-operated small operation chambers can be reduced through the independent small covers. The device disclosed by the invention is a plastic product, can be repeatedly used an also can be disposably used.
Description
Technical field
The present invention relates to be used for the manipulation in vitro of mammal embryo and the device of cultivation.
Background technology
At present; The device that is exclusively used in mammal embryo manipulation in vitro, cultivation seldom; External washing generally adopts petridish micro drop method and glass to pick up egg apparatus, and micro drop method is easy and simple to handle, but it is less to cultivate drop; Will thoroughly wash cultivation needs a large amount of drop and time-consuming, prolongs the embryo in the external residence time.Glass is picked up egg apparatus and is used up all at every turn and will reuse, if washing thoroughly can not influence fetal development, the single egg apparatus of picking up is inconvenient to use simultaneously.
Summary of the invention
Technical problem to be solved by this invention provides a kind of device that is used for animal embryo manipulation in vitro and cultivation, and it carries out embryo's washing fast and more quickly in embryo operation, improve embryo development rate.
The inventive system comprises petridish and petridish lid, and petridish comprises the operation cell that is used for embryo's manipulation in vitro.
Preferably, said operation cell sidewall linearly or circular-arc doline, be used to make the embryo along the sidewall landing to operating the cell bottom.Further preferably, the little and level of said operation cell base diameter.
Preferably, said petridish also comprises the operating gear pallet, and the said operation cell of plurality of rows is set in said operating gear pallet.
Preferably, said petridish lid comprises the operation cell lid that several can independently open, and each operation cell lid is used to cover row's operation cell.
Preferably, said device is plastics, and can repeat or disposable use.
Positive progressive effect of the present invention is: (one) the present invention is used for the device of mammal embryo manipulation in vitro; When having overcome the outer operational cost of traditional embryoid body with inconvenient shortcoming; Because the open ended operation liquor ratio of operation cell is more; The embryo also is washing embryo's a process by the process of operation liquid top free subsidence to the bottom, need not carry out over-drastic piping and druming to the embryo.(2) the present invention operates little locular wall and is doline, but the embryo on the cell sidewall along the sidewall landing to the bottom; Little and the level of cell base diameter, the embryo is easier in absorption.(3) the petridish lid has the independent tegillum that six rows can open, and when purpose is operation one row's operation cell, can reduce the moisture evaporation of other row's operation cells.(4) the present invention is plastics, can repeat practicality and also can disposablely use.
Description of drawings
Below in conjunction with accompanying drawing and embodiment the present invention is done further detailed explanation:
Fig. 1 is the structural representation of the petridish of the embodiment of the invention;
Fig. 2 is the structural representation of the operation cell of the embodiment of the invention;
Fig. 3 is the structural representation of the petridish lid of the embodiment of the invention;
Fig. 4 is the working state schematic representation of the embodiment of the invention.
Embodiment
In order to make those skilled in the art person understand technical scheme of the present invention better, and make above-mentioned purpose of the present invention, feature and advantage can be more obviously understandable, below in conjunction with embodiment and embodiment accompanying drawing the present invention done further detailed explanation.
The present invention is to provide a kind of device that is used for mammal embryo manipulation in vitro and cultivation, it carries out embryo's washing fast and more easily in embryo operation, improve embryo development rate.This device comprises petridish 1 and petridish lid 2.Fig. 1 is the structural representation of the petridish 1 of the embodiment of the invention, comprises operating gear pallet 10, and the said operation cell 11 of plurality of rows is set in said operating gear pallet 10, is 4 operations of the every row of 6 rows cell 11 in this example.Operation cell 11 is used for embryo's manipulation in vitro; Because the operation liquor ratio that operation cell 11 is held is more; The embryo is by the process of operation liquid top free subsidence to the bottom; Also be washing embryo's process, do not need the polyembryony tire to carry out over-drastic piping and druming, when having overcome the outer operational cost of traditional embryoid body with inconvenient shortcoming.Fig. 2 is the structural representation of the operation cell 11 of the embodiment of the invention, and said operation cell 11 is trapezoidal, sidewall 11a linearly or circular-arc doline, be convenient to make the embryo along the sidewall landing to operating cell bottom 11b.Little and the level of said operation cell bottom 11b diameter, the embryo is easier in absorption.
Fig. 3 is the structural representation of the petridish lid of the embodiment of the invention.Said petridish lid 2 comprises the operation cell lid 21 that several can independently open, and shown in the figure is 6, can increase and decrease its quantity according to actual needs.Each operation cell lid 21 is used to cover row's operation cell 11.Because each operation cell lid 21 can independently be opened and cover; Working state figure as shown in Figure 4; During with suction embryonic tube 3 operations one row's operation cell 11; Only need open a corresponding operation cell lid 21, and other operation cell lid keeps covering state, thereby can reduce the moisture evaporation of other row's operation cells.
Positive progressive effect of the present invention is: (one) the present invention is used for the device of mammal embryo manipulation in vitro; When having overcome the outer operational cost of traditional embryoid body with inconvenient shortcoming; Because the open ended operation liquor ratio of operation cell is more; The embryo also is washing embryo's a process by the process of operation liquid top free subsidence to the bottom, does not need the polyembryony tire to carry out over-drastic piping and druming.(2) the present invention operates little locular wall and is doline, but the embryo on the cell sidewall along the sidewall landing to the bottom; Little and the level of cell base diameter, the embryo is easier in absorption.(3) the petridish lid has the independent tegillum that six rows can open, and when purpose is operation one row's operation cell, can reduce the moisture evaporation of other row's operation cells.(4) the present invention is plastics, can repeat practicality and also can disposablely use.
The above is merely embodiment of the present invention, and the present invention can also be applied in other opertaing device.Protection scope of the present invention is not limited thereto, and any technician who is familiar with the present technique field is in the technical scope that the present invention discloses, and the variation that can expect easily or replacement all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain that claim was defined.
Claims (6)
1. a device that is used for animal embryo manipulation in vitro and cultivation is characterized in that: comprise petridish and petridish lid, and petridish comprises the operation cell that is used for embryo's manipulation in vitro.
2. device according to claim 1 is characterized in that: said operation cell sidewall linearly or circular-arc doline, be used to make the embryo along the sidewall landing to operating the cell bottom.
3. device according to claim 2 is characterized in that: the little and level of said operation cell base diameter.
4. device according to claim 1 is characterized in that: said petridish also comprises the operating gear pallet, and the said operation cell of plurality of rows is set in said operating gear pallet.
5. device according to claim 1 is characterized in that: said petridish lid comprises the operation cell lid that several can independently open, and each operation cell lid is used to cover row's operation cell.
6. according to any described device in the above claim, it is characterized in that: said device is plastics, and can repeat or disposable use.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104570585A CN102604830A (en) | 2011-12-19 | 2011-12-19 | Device for operating and culturing animal embryo in vitro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104570585A CN102604830A (en) | 2011-12-19 | 2011-12-19 | Device for operating and culturing animal embryo in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102604830A true CN102604830A (en) | 2012-07-25 |
Family
ID=46522608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104570585A Pending CN102604830A (en) | 2011-12-19 | 2011-12-19 | Device for operating and culturing animal embryo in vitro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102604830A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2047666U (en) * | 1989-05-25 | 1989-11-15 | 天津市第二医学院医药科技咨询服务开发公司 | Sectional culture dish made of thin plastic |
| CN1989237A (en) * | 2004-06-24 | 2007-06-27 | 雅各布·莫伦巴克有限公司 | Culture dish for culturing biological cells |
| CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
| CN201713520U (en) * | 2010-06-23 | 2011-01-19 | 西南大学 | Culture dish for collecting and culturing microorganism outdoors |
-
2011
- 2011-12-19 CN CN2011104570585A patent/CN102604830A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2047666U (en) * | 1989-05-25 | 1989-11-15 | 天津市第二医学院医药科技咨询服务开发公司 | Sectional culture dish made of thin plastic |
| CN1989237A (en) * | 2004-06-24 | 2007-06-27 | 雅各布·莫伦巴克有限公司 | Culture dish for culturing biological cells |
| CN201695031U (en) * | 2010-05-17 | 2011-01-05 | 复旦大学 | Partitioned culture vessel with glass bottom |
| CN201713520U (en) * | 2010-06-23 | 2011-01-19 | 西南大学 | Culture dish for collecting and culturing microorganism outdoors |
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| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20120725 |
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| C20 | Patent right or utility model deemed to be abandoned or is abandoned |