CN211170711U

CN211170711U - Positioning support and electric signal detection device

Info

Publication number: CN211170711U
Application number: CN201921841434.9U
Authority: CN
Inventors: 严颖刚; 范靖; 邹潭
Original assignee: Zhejiang Hopstem Bioengineering Co ltd
Current assignee: Zhejiang Hopstem Bioengineering Co ltd
Priority date: 2019-10-29
Filing date: 2019-10-29
Publication date: 2020-08-04
Anticipated expiration: 2029-10-29

Abstract

The utility model relates to a locating support and signal of telecommunication detection device. This locating support installs in the electrode hole of electrode board, includes: a hollow structure for housing a 3D organoid, the hollow structure comprising a placement space proximate to the electrode plate, the placement space configured to match a shape of the 3D organoid; and at least part of the outer wall of the hollow structure is provided with an abutting part, and the abutting part is used for abutting against the inner wall of the electrode hole when the positioning bracket is arranged in the electrode hole. The positioning bracket provided by the utility model has stable and reliable positioning, can make the 3D organs accurately contact with the central electrode at the bottom of the electrode hole, collects stable and repeatable electric signals and improves the detection precision of the electric signals; the positioning bracket is convenient to take and place, and the electric signal detection efficiency can be improved; different positioning supports are respectively arranged in different electrode holes, so that electric signal detection can be simultaneously carried out on different 3D organs, and the 3D organs can be conveniently detected in batches.

Description

Positioning support and electric signal detection device

Technical Field

The utility model relates to a bioengineering uses technical field, concretely relates to locating support and signal of telecommunication detection device.

Background

The existing microelectrode array (MEA) porous plate is only suitable for detecting extracellular electric signals of 2D cells and is not suitable for detecting electric signals of 3D organs. At present, 3D organs need to be adhered to electrodes at the bottoms of the holes of the MEA by means of matrigel in the 3D organ MEA detection, the process is time-consuming and labor-consuming, and the matrigel is easy to fall off. In addition, considering that the number of the bottom electrodes of the holes of the MEA is limited and the bottom electrodes are non-uniformly distributed, 1 3D-type organ needs to be adhered to the electrode area of each hole to ensure that the electrical signal is received, but the technical difficulty of the process is high, and stable collection and batch detection of the electrical signal cannot be ensured.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned problem that prior art exists, the utility model discloses an aspect's aim at provides a locating support to realize the effect that quick, stable detection and batchization of 3D class organ signal of telecommunication detected.

In order to achieve the above object, a first aspect of the present invention provides a positioning bracket, installed in an electrode hole of an electrode plate, including:

a hollow structure for housing a 3D organoid, the hollow structure comprising a placement space proximate to the electrode plate, the placement space configured to match a shape of the 3D organoid;

and at least part of the outer wall of the hollow structure is provided with an abutting part, and the abutting part is used for abutting against the inner wall of the electrode hole when the positioning bracket is arranged in the electrode hole.

In some embodiments, the hollow structure comprises a hollow cylinder, a plurality of positioning plates are arranged on one side of the cylinder in the axial direction, which is close to the electrode plate, and the plurality of positioning plates are uniformly distributed along the radial direction of the cylinder to form the placing space; the outer wall of barrel is equipped with a plurality of backup pads, and is a plurality of the backup pad is followed the radial direction equipartition of barrel forms a plurality ofly butt portion.

In some embodiments, the support plate includes a first support portion located outside the cylinder and a second support portion located inside the cylinder, the first support portion being capable of abutting against an inner wall of the electrode hole, and a bottom of the second support portion being connected to a top of the positioning plate.

In some embodiments, the axial dimension of the abutment is greater than the axial dimension of the barrel.

In some embodiments, the barrel, the positioning plate and the support plate are of a unitary structure.

In some embodiments, the height of the positioning support is less than the hole depth of the electrode hole.

In some embodiments, the electrode plate is an MEA electrode plate, which is a 48-well plate.

In some embodiments, the positioning bracket is made of a transparent polystyrene material.

A second aspect of the present invention provides an electrical signal detection device, which includes the above positioning bracket and also includes the electrode plate; wherein:

the bottom of the electrode hole of the electrode plate is provided with a central electrode for electrically connecting the 3D organs.

In some embodiments, the electrode plate has a plurality of electrode holes, and a plurality of positioning brackets are respectively and correspondingly installed in each of the electrode holes.

Compared with the prior art, the embodiment of the utility model provides a positioning support, through set up the space of placing that is used for holding 3D class organ on hollow structure to fix positioning support in the electrode hole of plate electrode through the butt portion that sets up on hollow structure's outer wall, the location is reliable and stable, can make 3D class organ and the accurate contact of the central electrode of electrode hole bottom, collects stable repeatable electric signal, can reduce the electrode contact failure that leads to because of matrix stickness reduces, improves 3D class organ's electric signal detection precision; the positioning support is convenient to take and place, and the electric signal detection efficiency can be improved. In addition, different positioning supports are respectively arranged in different electrode holes, so that electric signals can be simultaneously detected on different 3D organs, and the 3D organs can be conveniently detected in batches.

Drawings

In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments, by way of example and not by way of limitation, and together with the description and claims, serve to explain the embodiments of the invention. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

Fig. 1 is a side view of a positioning bracket according to an embodiment of the present invention;

fig. 2 is a side view of another positioning bracket according to an embodiment of the present invention;

fig. 3 is a bottom view of the positioning bracket according to an embodiment of the present invention;

fig. 4 is an assembly diagram of the positioning bracket according to the embodiment of the present invention.

Reference numerals:

10-positioning bracket, 101-placing space, 102-abutting part, 1-cylinder, 2-positioning plate, 3-supporting plate, 31-first supporting part and 32-second supporting part;

20-electrode plate, 201-electrode hole, 2011-center electrode.

Detailed Description

In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined below to clearly and completely describe the technical solution of the embodiments of the present invention.

Unless defined otherwise, technical or scientific terms used herein shall have the ordinary meaning as understood by those of ordinary skill in the art to which the invention belongs. The use of "first," "second," and similar terms in the description herein do not denote any order, quantity, or importance, but rather the terms are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element or item listed before the word covers the element or item listed after the word and its equivalents, but does not exclude other elements or items. The terms "connected" or "coupled" and the like are not restricted to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

To maintain the following description of the embodiments of the present invention clear and concise, detailed descriptions of well-known functions and components may be omitted.

Fig. 1 to 4 show the structure schematic diagram of the positioning bracket of the embodiment of the present invention. As shown in fig. 1 to 4, the embodiment of the present invention provides a positioning bracket 10, which is installed in an electrode hole 201 of an electrode plate 20, and the positioning bracket 10 includes:

a hollow structure for accommodating a 3D organoid, the hollow structure comprising a placement space 101 proximate to the electrode plate 20, the placement space 101 configured to match a shape of the 3D organoid;

at least part of the outer wall of the hollow structure has an abutting portion 102, and the abutting portion 102 is configured to abut against the inner wall of the electrode hole 201 when the positioning holder 10 is mounted in the electrode hole 201.

The embodiment of the utility model provides a locating support 10, through set up the space of placing 101 that is used for holding 3D organoid on hollow structure, and in being fixed in locating support 10 electrode hole 201 of plate electrode 20 through butt portion 102 that sets up on hollow structure's outer wall, the location is reliable and stable, can make 3D organoid and the accurate contact of central electrode 2011 bottom electrode hole 201, collect stable and repeatable's signal of telecommunication, reduce the electrode contact failure who leads to because of the reduction of matrix stickness, improve 3D organoid's signal of telecommunication detection precision. In addition, the positioning support 10 is convenient to take and place, and the electric signal detection efficiency of the 3D organs can be improved.

In some embodiments, as shown in fig. 1 to 3, the hollow structure includes a hollow cylinder 1, one axial side of the cylinder 1 near the electrode plate 20 is provided with a plurality of positioning plates 2, the plurality of positioning plates 2 are uniformly distributed along a radial direction of the cylinder 1 to form a placement space 101 matching with the shape of the 3D organoid, and the placement space 101 is axially communicated with the hollow cylinder 1; the outer wall of barrel 1 is equipped with a plurality of backup pads 3, and a plurality of butt portions 102 are formed along the radial direction equipartition of barrel 1 to a plurality of backup pads 3.

In specific implementation, at least 3 positioning plates 2 are arranged to ensure stable positioning. In this embodiment, barrel 1 is close to axial one side of plate electrode 20 and is equipped with four locating plates 2 along the radial direction equipartition of barrel 1, and the contained angle between adjacent locating plate 2 is 90, and the one end that four locating plates 2 kept away from the inner wall of electrode hole 201 is mutually supported and is formed and place space 101, can fix a position 3D organoid. In this embodiment, the cylinder 1 is a hollow cylindrical cylinder, and in other embodiments, the cylinder 1 may also be cylindrical, square, or the like.

In some embodiments, as shown in fig. 1 and 2, the supporting plate 3 includes a first supporting portion 31 located outside the cylinder 1 and a second supporting portion 32 located inside the cylinder 1, the first supporting portion 31 can abut against the inner wall of the electrode hole 201, and the bottom of the second supporting portion 32 is connected with the top of the positioning plate 2.

In this embodiment, the support plate 3 is a wing plate, the wing plate penetrates through the inner wall of the cylinder 1 along the radial direction of the cylinder 1 to form a first support portion 31 located outside the cylinder 1 and a second support portion 32 located inside the cylinder 1, the first support portion 31 forms an abutting portion 102 for abutting against the inner wall of the cylinder 1, and the second support portion 32 is used for being connected with the positioning plate 2. The wing plate can fix the positioning support 10 and can be connected with the positioning plate 2, and the reliability and stability of positioning of the 3D organs can be guaranteed.

Further, the height of the wing plate is gradually reduced from outside to inside along the radial direction of the barrel body 1, the firmness of fixation of the positioning support 10 can be ensured due to the higher height of the first supporting part 31 of the wing plate positioned outside the barrel body 1, the lower height of the second supporting part 32 of the wing plate positioned in the barrel body 1 facilitates the placement of the 3D organs in the placement space 101, materials are saved, and the processing cost can be reduced. In addition, the inclined slope of the second support portion 32 can play a guiding role to make the 3D organoid quickly slide down into the placing space 101.

In other embodiments, the first supporting portion 31 and the second supporting portion 32 may be a split structure and are respectively disposed on the outer wall of the cylinder 1 and the inside of the cylinder 1.

In some embodiments, as shown in fig. 1, the positioning plate 2 is located outside the cylinder 1, i.e. the placing space 101 is located outside the cylinder 1 and is in axial communication with the hollow cylinder 1; in other embodiments, as shown in fig. 2, one part of the positioning plate 2 is located inside the cylinder 1, the other part is located outside the cylinder 1, the positioning plate 2 extends into the cylinder 1 from the bottom of the cylinder 1 and is connected with the inner wall of the cylinder 1 through the second supporting portion 32, and at this time, the placing space 101 is partially located inside the cylinder 1, so that the 3D organs placed from the top of the cylinder 1 can be guided.

In some embodiments, as shown in fig. 1 and 2, the supporting plate 3 may be integrally formed with the barrel 1 during processing, and the second supporting portion 32 located in the barrel 1 extends downward for a certain distance to form the positioning plate 2, i.e. the barrel 1, the positioning plate 2 and the supporting plate 3 are an integral structure. Establish locating support 10 into integral type structure, be convenient for fix a position fast, improve signal of telecommunication detection efficiency.

In other embodiments, a placing space 101 for accommodating a 3D organ can be directly machined at the axial end of the cylinder 1 close to the electrode plate 20, the bottom of the placing space 101 can be in contact with the central electrode of the electrode plate 20, and the outer wall of the cylinder 1 is provided with an abutting part 102 which can abut against the inner wall of the electrode hole 201. The specific shape and size of the positioning support 10 are set according to the shape and size of the electrode holes 201 and the 3D organoid, and the utility model is not limited in particular.

In some embodiments, as shown in fig. 1 and 2, the axial dimension of the abutment 102 is greater than the axial dimension of the cartridge 1. In this embodiment, the height of pterygoid lamina is greater than the length of barrel 1 for the support intensity between pterygoid lamina and the inner wall of electrode hole 201 is greater than the maximum deformation degree of barrel 1, can prevent the upset of locating support 10, guarantees that it is reliable and stable.

In some embodiments, the height of the positioning bracket 10 is less than the hole depth of the electrode hole 201. Preferably, the depth of the electrode hole 201 is 12-15 mm, and the height of the positioning support 10 is 6-10 mm, so that the positioning support 10 can be conveniently and quickly taken and placed.

In some embodiments, the electrode plate 20 is an MEA electrode plate, which may be a 48-well plate, or other standard MEA electrode plate.

In some embodiments, the positioning frame 10 is made of a biocompatible material, such as a transparent polystyrene material, and has good processability and rigidity.

The embodiment of the utility model also provides an electric signal detection device, which comprises the positioning bracket 10 and the electrode plate 20; wherein: the bottom of the electrode hole 201 of the electrode plate 20 has a central electrode 2011 (electrode focus) for electrically connecting 3D organoids.

As shown in fig. 4, the electrode plate 20 has a plurality of electrode holes 201, and a plurality of positioning brackets 10 are respectively installed in each of the electrode holes 201. Different positioning supports 10 are respectively arranged in different electrode holes 201, so that electric signals of different 3D organs can be detected simultaneously, and batch detection of the 3D organs is facilitated.

The electrical signal detection device further includes a top cover (not shown) covering the electrode plate 20 for sealing the electrode hole 201 for easy transportation.

The embodiment of the utility model provides an electric signal detection device's theory of operation as follows:

first, matrigel is laid on the electrode plate 20. Specifically, matrigel is laid at the central electrode 2011 at the bottom of the electrode plate 20, the electrode plate 20 is shaken in a cross shape (the electrode plate 20 is shaken for several times in the front-back direction and the left-right direction) so that the matrigel is uniformly distributed on the central electrode 2011 of the electrode plate 20, a top cover is covered, the electrode plate 20 assembled in a whole is transferred into a 37 ℃ incubator and is placed for a period of time (for example, 1 hour) and then is coated, and the coated electrode plate is used after coating is completed.

Next, the positioning bracket 10 is placed. After the coating of the matrix glue is completed, the encapsulation solution is discarded, and the sterile positioning brackets 2 are carefully placed one by one in the electrode holes 201 of the electrode plate 20 with clean tweezers.

Thereafter, batch inoculation of 3D organoids was performed. The 3D organoid is added into the placing space 101 from the upper part of the positioning support 10 by using a pipette, the 3D organoid slides down along the placing space 101 to the bottom of the electrode holes 201 to be contacted with the central electrode 2011, a very small amount of culture medium (for example, 5ul) is kept in each electrode hole 201, the top cover is covered, the electrode plate 20 assembled in a whole is transferred to a 37 ℃ incubator to be kept still for a period of time (for example, 1 hour), and then 200 ul/hole culture medium is added into the electrode holes 201 to culture the 3D organoid. In this example, a 2ml pipette was used as the pipette.

And finally, carrying out batch electric signal detection on the 3D organoids. After the 3D organs in the electrode holes 201 are cultured to a certain degree, the top cover, the positioning bracket 10 and the electrode plate 20 which are assembled into a whole are placed in an MEA (membrane electrode assembly) instrument, and then batch detection of electric signals can be carried out.

The above embodiments are only exemplary embodiments of the present invention, and are not intended to limit the present invention, and the protection scope of the present invention is defined by the claims. Various modifications and equivalents of the invention can be made by those skilled in the art within the spirit and scope of the invention, and such modifications and equivalents should also be considered as falling within the scope of the invention.

Claims

1. The utility model provides a locating support installs in the electrode hole of electrode plate which characterized in that includes:

2. The positioning bracket according to claim 1, wherein the hollow structure comprises a hollow cylinder, a plurality of positioning plates are arranged on one side of the cylinder in the axial direction, which is close to the electrode plate, and the plurality of positioning plates are uniformly distributed along the radial direction of the cylinder to form the placing space; the outer wall of barrel is equipped with a plurality of backup pads, and is a plurality of the backup pad is followed the radial direction equipartition of barrel forms a plurality ofly butt portion.

3. The positioning bracket according to claim 2, wherein the support plate comprises a first support part located outside the cylinder and a second support part located inside the cylinder, the first support part being capable of abutting against an inner wall of the electrode hole, and a bottom of the second support part being connected to a top of the positioning plate.

4. A positioning bracket according to claim 2, wherein the axial dimension of the abutment is greater than the axial dimension of the barrel.

5. The positioning bracket of claim 2, wherein the barrel, the positioning plate and the support plate are of a unitary construction.

6. The positioning bracket according to any one of claims 1 to 5, wherein the height of the positioning bracket is smaller than the hole depth of the electrode hole.

7. The positioning bracket according to any one of claims 1 to 5, wherein the electrode plate is an MEA electrode plate, and the MEA electrode plate is a 48-orifice plate.

8. The positioning frame according to any one of claims 1 to 5, wherein the positioning frame is made of a transparent polystyrene material.

9. An electric signal detection device, comprising the positioning bracket according to any one of claims 1 to 8, and further comprising the electrode plate; wherein:

10. The electrical signal detecting device according to claim 9, wherein the electrode plate has a plurality of electrode holes, and a plurality of positioning brackets are respectively and correspondingly mounted in each of the electrode holes.