491941 A7 __B7 五、發明説明（1 ) 發明背景 發明領域 本發明一般係有關分析化學樣本，而更明確地，係有 關一種具有三維光線成形（shaping)樣本表面之分析基板 (substrate )及製造此一基板之方法。 2. 習知技術之討論 基板被廣泛地使用於化學測試及檢驗。使用這些測試 ，則化學物可被正確地量化及特徵化。這些測試被使用於 實驗室硏究及許多常見疾病的臨床診斷，例如癌症、炎症' 性疾病、AIDS、肝炎、及免疫性疾病。每年在美國大約有 五千萬個分析盤被使用。逾1 9 9 6年，各種分析盤之市場大 約爲八千萬元。然而，傳統的平面盤具有極小的表面以利 附著樣本。 雖然目前的塑膠製品可應用於各種形狀及尺寸，但是 塑膠製品具有平坦的二維表面而只有受限的表面積。已嘗 試數種方法以增加塑膠製品之表面積。此外，可構成具溝 槽的孔槽（well )狀底部，其增加孔槽之表面積。 目前，實驗室塑膠製品具有多種形狀，包含燒瓶、有 蓋培養皿、多孔槽盤（包含微滴量盤）、滾筒瓶、及試管 .。組織培養碟具有35、60、90、100及145之直徑，具有多種 修飾（例如內部孔槽及柵格）。通常，此種塑膠製品具有 通風良好的蓋子以利足夠的氣體交換，但是有最少量的蒸 本紙張尺度適用中國國家標準（CNS ) A4規格（：21〇X297公酱.) ΤΖΊ -- I--------裝—— (請先閱讀背面之注意事項再填寫本頁) 訂 經濟部智.慧財產局員工消費合作社印製 491941 A7 B7 五、發明説明（2 ) 發。多孔槽盤可具有數種不同形狀的底部，包含平的、圓 的、V形及U形的底部。 此外，當今的微滴量盤被製成具有增加的孔槽數目。 例如某一製造條件提供一種用於高密度細胞或分子檢驗之 每立方公分具有4，3 5 6孔槽的分析盤。孔槽之密度的此種極 大增加造成孔槽尺寸及表面積之伴隨的減少。孔槽尺寸及 表面積之此減少造成增加孔’槽表面積之需求。 對於使用與分析儀器之裝置，例如高壓液態色層分析 (HPLC )及氣體液態色層分析（GLC )，其中化學物檢測 係根據其接觸與一物質之基板，此等基板因其平坦表面之 有限的表面積而限制了此分析，且同樣情形發生於使用此 等分析儀器之平順的球形表面或基板。 對於化學檢驗所需要者爲一種具有較大表面積之基板 。此外，有需要一種可被製作以增加基板之表面積的基板 〇 此外，分析裝置及樣本可被照明以利使用吸附、螢光 及冷光分析技術之分析。於習知技術中，其使用入射光光 度測定之螢光及冷光係頂級的分析技術。傳輸之照明，即 ，淸晰的底盤，容許底部檢測器配置。然而，習知技術之 淸晰底盤未提供發出自樣本之光學成形，且無法減少來自 分析盤之來源光線的反向散射。光線之反射/折射、散射 、錯誤指向，及其他光學損失導致僅有傳輸照明之一小部 分到達檢測器，而降低讀取正確性及讀取時間。同時’隨 著微滴量盤中之孔槽的增加密度’而導致每單位孔槽之顯 本紙張尺度適用中.國國家標準（CNS ) A4規格（210 X 297公釐·） . 5 - I i- (請先閱讀背面之注意事項再填寫本頁) ♦ 訂 經濟部智慧財產局員工消費合作社印製 491941 A7 ___ B7 ___ 五、發明説明（3 ) 著較少的照明面積。· 因此，需要一種具有射出自樣本之光線的光學成形之 分析裝置，其減少來自分析盤之來源光的反向散射。 發明槪述 本發明之一目的係提供三維的光成形結構以增進化學 樣本分析。光成形結構提升樣本之黏著性及分析。依據本 發明之第一型態，此目的被達成，藉由使用一種分析裝置 ，其包括一大致上光學透明的物質，此裝置具有頂部及底 部表面且頂部表面被形成以包含三維的光成形結構。 本發明之另一目的係提供一種用以聚集化學物之裝置 及方法。依據本發明之此型態，化學物被聚集於一具有三 維光成形結構之裝置上，其增加有利於化學物聚集之表面 積。 本發明之又另一目的係提供一種包括大致上光學透明 之基板的微滴量盤，此基板具有第一及第二表面以及多數 分析孔槽於第一表面中，每個孔槽具有一側壁及一底部以 界定一分柝室，且其中底部表面被形成以包含一三維的光 成形結構。 本發明之再另一目的係提供一種包括大致上光學透明 單片（monol.hh )，此單片具有頂部及底部表面且被形成以 包含三維的光成形結構及一孔槽陣列（其具有第一及第二 側邊及多數通道形成於其中），每個通道具有第一及第二 開口端及一側壁，而其中孔槽陣列被固定至頂部表面以使 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐) ^7] " --------"裝-- (請先閱讀背面之注意事項再填寫本頁)491941 A7 __B7 V. Description of the Invention (1) Background of the Invention The present invention relates generally to analyzing chemical samples, and more specifically, relates to an analysis substrate having a three-dimensional light-shaping sample surface and manufacturing the same. Substrate method. 2. Discussion of conventional technologies Substrates are widely used in chemical testing and inspection. Using these tests, chemicals can be properly quantified and characterized. These tests are used in laboratory investigations and clinical diagnosis of many common diseases, such as cancer, inflammatory diseases, AIDS, hepatitis, and immune diseases. Approximately 50 million analytical disks are used in the United States each year. For more than 196 years, the market for various analytical disks was approximately RMB 80 million. However, conventional flat disks have extremely small surfaces to facilitate sample attachment. Although current plastic products can be applied to various shapes and sizes, plastic products have flat two-dimensional surfaces and have limited surface area. Several methods have been tried to increase the surface area of plastic products. In addition, a well-shaped bottom with a groove can be formed, which increases the surface area of the well. At present, laboratory plastic products come in various shapes, including flasks, Petri dishes with lids, multiwell trays (including microtiter disks), roller bottles, and test tubes. Tissue culture dishes have diameters of 35, 60, 90, 100, and 145, with various modifications (such as internal slots and grids). Generally, this kind of plastic product has a well-ventilated cover to facilitate sufficient gas exchange, but the minimum amount of steamed paper is applicable to the Chinese National Standard (CNS) A4 specification (: 21〇297297 sauce). ΤZZΊ-I- ------- Installation—— (Please read the precautions on the back before filling this page) Order printed by the Ministry of Economic Affairs and Intellectual Property Bureau employee consumer cooperative 491941 A7 B7 V. Inventory Note (2) issued. Multiwell trays can have several different shaped bottoms, including flat, round, V-shaped and U-shaped bottoms. In addition, today's microtiter disks are made with an increased number of wells. For example, a manufacturing condition provides an analytical disk with a high density of 4, 3 6 6 wells per cubic centimeter for high density cell or molecular testing. This extreme increase in the density of the pores and grooves results in a concomitant decrease in the size and surface area of the pores and grooves. This reduction in cell size and surface area creates a need to increase the surface area of cells. For devices using and analyzing instruments, such as high-pressure liquid chromatography (HPLC) and gas liquid chromatography (GLC), chemical detection is based on substrates that are in contact with a substance. These substrates are limited by their flat surfaces. This surface area limits the analysis, and the same situation occurs with smooth spherical surfaces or substrates using these analytical instruments. What is needed for chemical inspection is a substrate with a large surface area. In addition, there is a need for a substrate that can be fabricated to increase the surface area of the substrate. In addition, the analysis device and sample can be illuminated to facilitate analysis using adsorption, fluorescence, and cold light analysis techniques. Among the conventional techniques, it uses the top-level analysis technology of fluorescence and cold light for photometric determination of incident light. The lighting of the transmission, ie, the clear chassis, allows the bottom detector to be configured. However, the conventional clear chassis does not provide optical shaping from the sample, and it cannot reduce backscatter of the source light from the analysis disc. Light reflection / refraction, scattering, misdirection, and other optical losses cause only a small portion of the transmitted illumination to reach the detector, reducing read accuracy and read time. At the same time, with the increase of the density of the wells and grooves in the micro-droplet, the paper size per unit of wells and grooves is applicable. The National Standard (CNS) A4 (210 X 297 mm ·). 5-I i- (Please read the notes on the back before filling out this page) ♦ Order printed by the Intellectual Property Bureau Employee Consumer Cooperative of the Ministry of Economic Affairs 491941 A7 ___ B7 ___ V. Invention Description (3) Less lighting area. Therefore, there is a need for an optically shaped analysis device having light emitted from a sample, which reduces backscatter of source light from an analysis disk. SUMMARY OF THE INVENTION It is an object of the present invention to provide a three-dimensional photoformed structure to enhance the analysis of chemical samples. The light-formed structure improves the adhesion and analysis of the sample. According to a first aspect of the invention, this object is achieved by using an analysis device comprising a substantially optically transparent substance, the device having a top and a bottom surface and the top surface being formed to contain a three-dimensional photo-forming structure . Another object of the present invention is to provide a device and method for accumulating chemicals. According to this aspect of the present invention, the chemicals are collected on a device having a three-dimensional photo-forming structure, which increases the surface area that facilitates the accumulation of the chemicals. Yet another object of the present invention is to provide a micro-droplet disk including a substantially optically transparent substrate, the substrate having first and second surfaces and a plurality of analysis wells in the first surface, each well having a side wall And a bottom to define a tiller chamber, and wherein the bottom surface is formed to include a three-dimensional photoforming structure. Still another object of the present invention is to provide a monolithic sheet (monol.hh), which has a top and a bottom surface and is formed to include a three-dimensional photoforming structure and a slot array (which has a One and second sides and a plurality of channels are formed therein), each channel has first and second open ends and a side wall, and the array of holes and grooves is fixed to the top surface so that the paper size applies the Chinese national standard (CNS ) A4 size (210X297mm) ^ 7] " -------- " equipment-(Please read the precautions on the back before filling this page)
、1T Φ 經濟部智慧財產局員工消費合作社印製 491941 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明説明（4 ) 得每個通道界定一由側壁及頂部表面所界定之液體密閉分 析室。 . 本發明之另一目的係提供一種包括大致上光學透明物 質之單片的分析燒瓶，此單片具有頂部及底部表面，其頂 部表面被形成以包含三維的光成形結構，及一分析室被固 定至該單片且置於頂部表面上而界定一液體密閉室。 本發明之另一目的係提供一種分析樣本之方法，其包 括下列步驟：提供一大致上光學透明之基板，此基板具有 第一及第二表面、及將樣本置入多數形成於第一表面中之 分析孔槽的至少一個，其每個孔槽具有一側壁及一底部以 界定一分析室且其中底部被形成以包含三維的光成形結構 、及黏附樣本至三維的光成形結構。 本發明之又另一目的係提供一種用於化學樣本分析之 裝置，其減少於樣本之光學分析期間來自光源的反向散射 ，而藉以增進射出自樣本之所欲光信號的檢測，且藉以增 加分析期間之信號雜訊比。 本發明之又另一目的係提供一種用於化學樣本分析之 裝置，其將射出自樣本之光線成形爲適當的光輸出分佈’ 以使得其射出自樣本之光線較少被浪費且較多可應用以被 檢測及分析。 ’ 依據本發明之又進一步目的，其基板以及分析孔槽中 之樣本被照射以來自一光源的光線，且光成形結構將其射 出自樣本之光線成形爲所欲的光輸出分佈，.其接著被檢測 及分析。 本紙張尺度適用中.國國家標準（CNS ) A4規格（210X 297公釐·） . J . -------裝-----r ---訂------- (請先閎讀背面之注意事項再填寫本頁) 491941 A7 B7 五、發明説明（5 ) (請先閲讀背面之注意事項再填寫本頁) 本發明之再另一目的係提供一種分析一分析基板上之 樣本的方法，其包括下列步驟：提供一大致上光學透明之 基板，其具有一表面被形成以包含三維的光成形結構、將 樣本放置於基板及三維的光成形結構上、提供一光源、以 光線照射基板及樣本、光成形結構將其射出自樣本之光線 成形爲所欲的光輸出分佈並減少其射入基板與樣本之光的 反向散射、及檢測與分析光輸出分佈。 本發明之其他目的、特徵及優點將由以下詳細敘述及 伴隨之圖形而爲那些熟悉本項技術者所淸楚瞭解。然而， 應注意到以下詳細敘述及特定範例（雖然表明本發明之較 佳實施例）係僅用於說明而非限制。其許多更改及修飾均 可被實施於本發明之範圍內而不背離其精神，且本發明包 含所有這些修飾。 圖形簡述 本發明之較佳實施例係參考伴隨之圖形而被說明，其 中類似的數字代表類似的部分，且其中： 經濟部智慧財產局員工消費合作社印製 圖1爲依據本發明之一較佳實施例所配置之微滴量盤的 透視圖； , 圖2係沿著圖1之線段2-2所取之橫斷面圖； 圖3係微.滴量盤之一平面圖，此微滴量盤具有依據本發 明之一第一替代較佳實施例而配置的分析孔槽； 圖4係微滴量盤之一平面圖，此微滴量盤具有依據本發 明之一第二替代較佳實施例而配置的分析孔槽； 本紙張尺度適财.關家縣（CNS ) A4規格（21GX297公釐) ^7] 491941 Μ ____ Β7 五、發明説明（6 ) 圖5係微滴量盤之一部分橫斷面圖’此微滴量盤具有依 據本發明之一第三替代較佳實施例而配置的分析孔槽； 圖6係依據本發明之一較佳實施例而配置之一分析燒瓶 的透視圖； 圖7係沿著圖6之線段7-7所取之橫斷面圖； 圖8係適於使用本發明之較佳實施例的分析系統之槪略 圖示； 圖9係依據本發明之一較佳實施例的光分佈型態之槪略 示圖； 圖1 0係三維光成形表面結構之照片，此結構係配置以 產生圖9中所不之光分佈型態； 圖1 1係依據本發明之一替代較佳實施例的光分佈型態 之槪略‘示圖； 圖1 2係二維光成形表面結構之照片’此結構係配置以 產生圖11中所不之光分佈型態； 圖1 3係依據本發明之一較佳實施例以形成微滴量盤的 裝置之槪略示圖； 圖1 4 A係依據本發明之一較佳實施例的三維結構之尺寸 特性的槪略示圖； 圖1 4B係依據本發明之一替代較佳實施例的三維結構之 尺寸特性的槪略示圖；及 圖1 5係一部分橫斷面圖以說明依據本發明之一替代較 佳實施例的分析盤。 本紙張尺度適用中國國家標準（〇泌）八4規格（210/297公釐） _ 9 - —------裝-- (請先閱讀背面之注意事項再填寫本頁) 訂 經濟部智慧財產局員工消費合作社印製 491941 A7 B7 五、發明説明（7 ) 經濟部智慧財產局員工消費合作社印製 元件對照表 100 微滴量盤 102 分析孔槽 104 側壁 106 基板 108 底部 200 模版 202 材料源 204 下模版印模 206 上模版印模 208 金屬主版 210 模版空腔 300 分析燒瓶 302 樣本盤 304 樣本表面 306 底部表面 308 三維表面結構 310 外殼 312 隙孔 314 插塞 400 分析裝置 402 盤 404 通道 406 頂部表面 (請先閱讀背面之注意事項再填寫本頁) 4 項再填、 裝· 訂 # 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐） _ 1〇 _ 491941 A7 B7 五、發明説明（8 ) 408 底部表面 410 內壁 412 三維的表面結構 414 封蓋 416 底部分 500 分析裝置 502 光源 504 檢測器 5 06 處理器 508 光分佈 較佳實施例之詳細敘述 1.包含三維結構之分析裝置 A. 微滴量盤 鑑於上述，並參考圖1及2，一微滴量盤1〇〇包含多數分 析孔槽1 0 2。每個分析孔槽1 0 2具有一般圓柱形側壁1 0 4延伸 於一大致上平面的基板106之上以界定一底部1〇8。微滴量 盤1 0 0最好是形成以一種塑膠材料，例如聚苯乙條、聚碳酸 鹽、聚氯乙烯（PVC )等等，其係鑑於光學傳輸性質而選取 。微滴量盤100可被形成以包含數個分析孔槽102，其中有 六個分析孔槽被顯示於圖1以利說明本發明。 雖然每個分析孔槽1 02被顯示具有圓柱形狀，應理解其 他的形狀亦可被使用而不背離本發明之一般範圍。例如， 參考圖3-5，其顯示有數種分析孔槽之替代配置，個別標示 本紙張尺度適用中國國家標準（0奶）八4規格（210、乂297公釐) (請先閱讀背面之注意事項再填寫本頁) 訂 經濟部智慧財產局員工消費合作社印製 491941 A7 ____ B7 五、發明説明（9 ) 以102’，102’’，102’’’。如圖3中所顯示，分析孔槽102’具有 圓形柱形狀。另一方面，每個分析孔槽102’’可具有稜柱形 狀（即，矩形形狀）以提高孔槽密度。同理如圖4中所顯示 之分析孔槽102’’，其具有角落爲半徑“r”之圓形的稜柱形狀 。當然若欲進一步增加孔槽密度，則可使用具有方形角落 之稜柱形狀孔槽。圖5顯示具有非正圓柱架構之分析孔槽 102…。亦即，底部108…未與側壁104…形成直角。反之， 分析孔槽1 0 2…具有一般爲“鬱金香”或拋物線狀的底部1 〇 8，，， 。應理解其數種額外的形狀包含“U”底部、“V”底部及其他可 用以增進孔槽密度、增加表面積、增加容量，等等，者， 以合乎特定分析應用之需求。再次參考圖2，每個底部1〇8 被形成以包含一三維的結構，其被配置以增進微滴量盤100 之光學’性質。 B. 製造三維的結構 已發現到有一種利用漫射器科技之三維結構，例如美 國專利編號5,3 6 5,3 5 4、5,5 3 4,3 8 6及5,609,93 9中所揭露的漫 射器結構，該等揭露被特地倂入於此以利參考，其提供一 * 種意外地對樣本有利的環境。如其中所述，其包括隨機、 混亂而非平面之污跡的三維光成形結構可被記錄並生長（ develop )於一光敏媒體中，以致其媒體之折射指數（例如 ，梯度折射指數）具有無間斷且平順的改變，其將準直的 光散射爲具有平順亮度變化之控制下的型態。以此方式形 成之三維的表面結構可被複製以任何數目的材料，透明或 ^氏張尺度適用中國國家標準（CNS ) A4規格（210X297公釐·) 一 I , (請先閲讀背面之注意事項再填寫本頁) 裝· 訂 經濟部智慧財產局員工消費合作社印製 491941 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明説明（10) 非透明，且接著被調適以形成底部1 08。 更明確地’ 一透過物鏡而耦ί合之相干（c 〇 h e r e n t )雷射 光可被使用以將來自主漫射器之三維表面結構記錄至光敏 媒體中。主漫射器可爲標準的地面玻璃漫射器、雙凸透鏡 漫射器、醋酸纖維漫射器或全像攝影漫射器。記錄於光敏 媒體中之表面特徵的尺寸、形狀及定向爲數項變數之函數 ，其變數包含所使用之物鏡及主漫射器的型式、以及那些 組件相對於彼此及相對於光敏媒體之相關位置。最後，所 欲之結果係透過經驗測試而獲得，而尤其，最適於黏附及/ 或培養特定樣本之三維結構將透過此等測試而達成。 有關對於三維表面結構之形成參數的效果之一般化聲 明可被陳述，且將促進選擇一種配置以獲得所欲的三維表 面結構。物鏡擴大相干光線以使得其來自物鏡而入射至主 漫射器上之光線的面積係大於雷射光束本身之橫斷面的面 積。藉由物鏡之小量放大率導致較小的入射於主漫射器上 。於此情況下，記錄於光敏媒體上之特徵係較大。因此， 入射至主漫射器上之光線的隙孔（aperture)的尺寸係反向 地關連與其記錄於光敏媒體中之表面特徵的尺寸。 介於物鏡與主漫射器之間的距離亦影響表面結構的尺 寸。當介於物鏡與主漫射器之間的距離減小時，則表面結 構之尺寸便增加。此情況之發生係因爲當物鏡移動更爲接 近主漫射器時，則入射至主漫射器之光線的外觀隙孔變得 較小。增加的結構尺寸提供具有減小光學漫射之三維表面 結構，亦即，較小的角度光線分佈。相反地，假如介於物 本紙張尺度適用中.國國家標準（CNS ) A4規格（210X297公釐） .13 - 丨 裝 „ 訂 I · (請先閲讀背面之注意事項再填寫本頁) 491941 A7 _ B7 五、發明説明（11) 鏡與主漫射器之間的距離增加時，則表面結構之尺寸減小 。較小的表面結構導致較大的角度散佈。 (請先閱讀背面之注意事項再填寫本頁) 介於主漫射器與光敏媒體之間的距離亦影響污跡尺寸 。當距離減少時，則記錄於光敏媒體中之污跡的尺寸亦減 小。此情況之發生係因爲，假設光線之一擴大光束被產生 於物鏡之上，當光敏媒體被移動更爲接近主漫射器時，則 其射出自主漫射器中之每個不規則處的光束將於其到達光 敏媒體之前較少量地擴大，因而產生較小的污跡。相反地 ，假如介於主漫射器與光敏媒體之間的距離增加，則所記 錄之污跡的尺寸將增大。因此，這些介於物鏡、主漫射器 、與光敏媒體之間的簡單關係均被（憑經驗地）調整，以 獲得光敏媒體中所欲的污跡尺寸，其因而決定從光敏媒體 所產生之漫射器的光輸出分佈，如以下進一步地說明。 經濟部智慧財產局員工消費合作社印製 光敏媒體亦可藉由使用其他方法（包含不相干光源及 接觸印刷處理程序）而被記錄，如1 99 8年8月20日申請之審 查中美國專利申請案序號09/ 1 37,3 9 7 (其中CPA申請案係申 請於2000年2月29日），以及1 99 8年5月8日申請之美國追加 專利申請案序號09/07 5，023 (現已放棄），該等揭露被特地 倂入於此以利參考。 一種具有理想光分佈型態之最佳的三維表面結構係使 用上述處理程序而被產生，且被用以產生一具有理想三維 表面結構修飾於其中之耐用的金屬主版，使用標準的電鍍 技術。更明確地，三維表面結構被記錄於光敏媒體中，其 接著被生長。從光敏媒體製成一複製結構，最好是藉由硬 1紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐） .14 _ 491941 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明説明（12) 化一層環氧化物於光敏物質中之三維的表面結構上。硬化 的環氧化物可被移除自光敏物質而留下三維的表面結構記 錄於其中。 一層薄的銀金屬被接著澱積且金屬化，於硬化的環氧 化物上。一陰極被接著耦合至銀，而一陽極被耦合至一鎳 條。塗敷銀之環氧化物及鎳條被接著浸入酸液中，且一電 流通過銀、酸液、及鎳條，而致使一層鎳形成於銀之上。 此層可生長至0.5英吋厚。環氧化物、銀及鎳結構被移除自 酸液，且環氧化物及銀被移除自鎳而留下一金屬主版，亦 即，一具有三維表面結構形成於其中之鎳墊片（shm)。 參考圖13，其顯示一包含以上述方式形成之金屬主版 208的微滴量模版200。模版200包含一下模版印模204及一上 模版印模206。金屬主版20 8被焊接、加熱、鍛接、接合或 其他適當方式以固定至下模版印模204，需謹慎操作以不破 壞其中所形成之三維的表面結構。模版空腔2 1 0被接著形成 於下模版印模204中，例如藉由放電加工（EDM )製造等等 。模版空腔界定微滴量盤之形狀的互補體（complement)。 如習知技術中所已知，上模版印模20 6包覆模版空腔2 1 0， 而塑膠材料從材料源202被射入模版空腔。應理解其模版空 腔2 10被形成以適當的起伏及牽引角度，以幫助移除已完成 的微滴量盤。此外，各種釋放混合物均可被使用。 C·包含三維光成形結構之單片、燒瓶、及聚集器 上述製造方法可被使用以形成包含微滴量盤100之多種 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公楚：） 丨 ^ ^ 訂 . I. (請先閲讀背面之注意事項再填寫本頁) 491941 A7 B7 經濟部智慧財產局員工消費合作社印製 五、發明説明（13) 架構的分析裝置。最簡單的此種結構係平坦分析盤，亦即 單片，其具有一表面被形成以包含三維的表面結構。參考 圖6及圖7，其顯示一種依據本發明之較佳實施例所構成的 分析燒瓶300。分析燒瓶300包含一樣本盤302。樣本盤302具 有一樣本表面304及一底部表面306。樣本表面304最好是被 形成以包含如此處所述之三維表面結構3 0 8。樣本表面3 0 4 被包覆以一光學透明的外殼310，其係密封樣本盤3 02之周 邊。一隙孔312被形成於外殼3.10中，而一插塞314被設置以 容許燒瓶300中之物質的引入或密封。當然，其中外殼310 僅用於生長細胞，其無需爲透明的。 參考圖.15，另一種分析裝置400被形成於盤402中。更 明確地，盤402被形成以包含至少一通道404延伸自一頂部 表面406經由盤402而至一底部表面408。通道404界定一內壁 4 10。如圖所示，通道404具有一錐形或“V”架構。應理解其 他的架構亦可被使用。同時，通道404可界定一細長狹縫於 盤402中，或者可界定一圓柱形或截頭圓錐形隙孔。內壁 4 1 0被形成以包含三維的表面結構4 1 2。樣本物質可通過通 道4 04而有一部分物質被黏著劑留住於三維的表面結構4 1 2 中。此外，一封蓋4 14可被設置以密封通道404之一底部分 416。以此一配置，通道404被構形爲一種具有樣本物質保 留於其中之分析孔槽。封蓋4 1 4可接著被移除以容許樣本物 質自通道404流出。因而可完成將樣本灌輸及流出或通過通 道4 04之數項步驟。三維表面結構412中之聚積的黏附樣本 物質可接著使用數種分析技術而被分析。 本^氏張尺度適用中國國家標準（CNS ) A4規格（210X297公釐） (請先閲讀背面之注意事項再填寫本頁) •裝· 訂 # 491941 經濟部智慧財產局員工消費合作社印製 A7 B7 五、發明説明（14) 參考圖8，顯示另一種示範的分析裝置5 00以分析其備 製於微滴量盤（例如微滴量盤1 〇 〇 )中之樣本。裝置5 0 0包 含一光源502、一檢測器504、及一處理器506。光源502被配 置以照射微滴量盤1 0 0之一個以上分析孔槽1 0 2以一相干雷 射光束。依據本發明之較佳實施例，來自被照射之分析孔 槽的光分佈5 0 8係藉由底部108中所形成之三維表面結構而 被光學地成形。對於微滴量盤1 00，其光分佈最好爲一種具 有角度Φ約爲1-10度範圍之環形分佈，而更理想的，對於 一種CCD檢測器，約爲2-3度範圍（參見圖9中所顯示之分佈 以及圖1 0中所顯示之相應結構）。藉由含入三維表面結構 於底部1 08中，則大致上有較多入射光被指向檢測器5 04， 而較少被浪費。檢測器504最好係一種配置有一耦合至處理 器5 06之輸出的電荷耦合裝置（CCD)，以處理及分析樣本 資料。 使用本發明之微滴量盤或者包含本發明之三維表面結 構的任何其他樣本盤或室之另一項重要優點在於：其來自 光源502而入射微滴量盤100之光不會反向散射朝向光源502 。反而，其係傳輸通過至樣本，其中光係相互作用與樣本 。如此增加其相互作用與及射出自樣本之光線的強度。 於任何型式之分光鏡系統中，可爲Raman (拉曼）或任 何其他型式，不希望有其偏離至樣本以外之光源波長的光 ，因爲如此會減低'系統之信號雜訊比’而更不易分辨其來 自樣本之所欲的光信號與其來自光源的光。尤其於拉曼分 光鏡中，以一波長些許偏移自光源之波長而射出自樣本的 本紙張尺度適用中國國家標準（CNS ) A4規格（210x297公董） -17- I----------r -- —IT!--― ;--.1 (請先閱讀背面之注意事項再填寫本頁) 491941 經濟部智慧財產局員工消費合作社印製 Α7 Β7 五、發明説明（15) 光線強度係較低的（相較於光源之強度），而使其不易檢 測出除了來源光以外之所欲的信號。低的信號雜訊比因而 爲光學樣本硏究之習知系統中的一個問題。因爲本發明之 分析'盤顯著地減低反向散射，所以本發明之分析盤可顯著 地增進大部分分光鏡系統中之信號雜訊比。 依據較佳實施例所形成之微滴量盤能夠傳輸超過百分 之80的入射光至檢測器504。反之，習知技術之微滴量盤， 因爲其反向散射及無法控制的漫射，只可傳輸少於百分之 1 0的入射光至檢測器5 0 4。此外，來自習知技術微滴量盤之 光的寬廣分佈可導致串擾（cross talk)於檢測器504上。亦 即，其相應於微滴量盤之其他分析孔槽的CCD之鄰近像素 可能被照射而導致錯誤指示。隨著較高密度的微滴量盤， 則變得越來越重要的是以一種受控制的方式指引一較高百 分比的入射光朝向檢測器。 如上所述，以此方式形成三維表面結構之一優點在於 其來自漫射器之光分佈型態可被控制。此係相反於傳統的 漫射器，其無法提供輸出光分佈之控制而僅提供朗伯（ Lambertian)漫射。參考圖9至12，其顯示一對光分佈型態 及一用以產生光分佈型態之相應的三維表面結構。更明確 地，參考圖9，其顯示一圓形的光分佈，其具有卜1〇度之角 度範圍Φ，而更理想的，約2-3度。其相應結構被顯示於圖 10之照片中。相反地，圖1 1顯示較寬角度（φ等於約2度相 較於φ等於約80度）之橢圓形光分佈型態。選擇一適當的 三維表面結構將依據數項因素，包含欲備製之樣本 '分析 本紙張尺度適用中國國家標準（CNS ) Α4規格（210X297公釐） -化_ 1 ------IT-----1--Ί (請先閲讀背面之注意事項再填寫本頁) 491941 經濟部智慧財產局員工消費合作社印制 A7 B7 五、發明説明（16) 技術及分析設備’包含所使用之檢測器尺寸及型態。 三維表面結構之兩個重要特性爲：縱橫比，即表面結 構之深度對高度的比率；及頂點比’即表面結構之深度對 直徑的比率。表面結構之尺寸特性被圖示以圖1 4 Α中之圓形 結構及圖1 4B中之橢圓結構。例如’ 1 : 1至1 : 6 (深度對高 度）範圍內之縱橫比，及1 : 0.5至1 : 1 (深度對直徑）範圍 內之頂點比可被使用。某些縱橫比及頂點比應根據其應用 而被選擇。舉例而言，數種結構之縱橫比及頂點比被描述 於表I中。應理解其三維的結構是完全隨機的，而因此，確 實的測量是不可能的。因此，某一結構之較佳縱橫比及頂 點比係藉由測量數個結構而被預測。應理解其他較大或較 小的縱橫比及頂點比可被使用，但是目前的製造限度會限 制較大的縱橫比。一種較佳的配置可利用大約1 -5微米高而 2-1 0微米寬之表面結構於細胞，而利用較小的表面結構於 分子。較大或較小的結構均可被使用。於任一情況下，最 佳的表面結構係根據樣本，而縱橫比被選擇以取得相關樣 本之最大表面積。此種表面結構及表面積之控制及最佳化 因此爲不可能的。此外，三維的表面結構可被形成以一有 利於樣本生長之大致上隨機的定向。 (請先閱讀背面之注意事項再填寫本頁) •裝-1T Φ Printed by the Employees 'Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 491941 Printed by the Employees' Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the invention (4) It is possible to define each channel a liquid-tight analysis defined by the side walls and the top surface room. Another object of the present invention is to provide an analysis flask including a single piece of substantially optically transparent substance, the single piece having a top and a bottom surface, the top surface of which is formed to contain a three-dimensional photoforming structure, and an analysis chamber It is fixed to the single piece and placed on the top surface to define a liquid-tight chamber. Another object of the present invention is to provide a method for analyzing a sample, which includes the following steps: providing a substantially optically transparent substrate having first and second surfaces, and placing a plurality of samples on the first surface At least one of the analysis slots has a sidewall and a bottom to define an analysis chamber, and the bottom is formed to include a three-dimensional photo-forming structure, and a sample is attached to the three-dimensional photo-forming structure. Yet another object of the present invention is to provide a device for chemical sample analysis, which reduces backscatter from a light source during optical analysis of a sample, thereby improving detection of a desired light signal emitted from the sample, and thereby increasing Signal to noise ratio during analysis. Yet another object of the present invention is to provide a device for chemical sample analysis, which shapes the light emitted from the sample into an appropriate light output distribution so that the light emitted from the sample is less wasted and more applicable To be detected and analyzed. According to a further object of the present invention, the sample in the substrate and the analysis slot is irradiated with light from a light source, and the light shaping structure shapes the light emitted from the sample into a desired light output distribution, and then Detected and analyzed. This paper size is applicable. National National Standard (CNS) A4 size (210X 297mm ·). J. ------- Packing ----- r --- Order ------- ( Please read the precautions on the back before filling out this page) 491941 A7 B7 V. Description of the invention (5) (Please read the precautions on the back before filling out this page) Another object of the present invention is to provide an analysis-analysis substrate The method of the above sample includes the following steps: providing a substantially optically transparent substrate having a surface formed to contain a three-dimensional light-forming structure, placing the sample on the substrate and the three-dimensional light-forming structure, and providing a light source , Irradiate the substrate and the sample with light, and the light shaping structure shapes the light emitted from the sample into a desired light output distribution and reduces the backscatter of the light incident on the substrate and the sample, and detects and analyzes the light output distribution. Other objects, features and advantages of the present invention will be apparent to those skilled in the art from the following detailed description and accompanying drawings. It should be noted, however, that the following detailed description and specific examples (although demonstrating preferred embodiments of the invention) are intended to be illustrative and not restrictive. Many changes and modifications thereof can be implemented within the scope of the present invention without departing from the spirit thereof, and the present invention includes all these modifications. Brief Description of the Drawings A preferred embodiment of the present invention is described with reference to the accompanying drawings, wherein similar numbers represent similar parts, and among them: Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs, Figure 1 A perspective view of a micro-disc disc configured according to the preferred embodiment; FIG. 2 is a cross-sectional view taken along line 2-2 of FIG. 1; FIG. 3 is a plan view of one of the micro-disc discs. The measuring disk has an analysis slot configured according to a first alternative preferred embodiment of the present invention; FIG. 4 is a plan view of a micro-droplet disk having a second alternative preferred implementation according to the present invention Analytical slots configured as an example; This paper is suitable for financial use. Guanjia County (CNS) A4 size (21GX297mm) ^ 7] 491941 Μ ____ B7 V. Description of the invention (6) Figure 5 is a part of a micro-droplet disk Cross-section view 'This micro-droplet disk has an analysis well configured according to a third alternative preferred embodiment of the present invention; Figure 6 is a perspective view of an analysis flask configured according to a preferred embodiment of the present invention Figure 7 is a cross-sectional view taken along line 7-7 of Figure 6; 8 is a schematic diagram of an analysis system suitable for using a preferred embodiment of the present invention; FIG. 9 is a schematic diagram of a light distribution pattern according to a preferred embodiment of the present invention; FIG. 10 is a three-dimensional diagram A photo of a light-formed surface structure, which is configured to produce a light distribution pattern not shown in FIG. 9; FIG. 11 is a schematic 'illustration of a light distribution pattern according to an alternative preferred embodiment of the present invention; Fig. 12 is a photo of a two-dimensional light-shaping surface structure. 'This structure is configured to produce a light distribution pattern not shown in Fig. 11. Fig. 13 is a diagram of a micro-droplet disc according to a preferred embodiment of the present invention. Schematic diagram of the device; FIG. 14A is a diagrammatic illustration of the dimensional characteristics of a three-dimensional structure according to a preferred embodiment of the present invention; FIG. 14B is a three-dimensional structure according to an alternative preferred embodiment of the present invention Figure 15 is a schematic diagram of the dimensional characteristics; and FIG. 15 is a cross-sectional view of a part to illustrate an analysis disk according to an alternative preferred embodiment of the present invention. The size of this paper is applicable to Chinese National Standard (〇 Bi) 8 4 specifications (210/297 mm) _ 9----------- installed-(Please read the precautions on the back before filling this page) Order Ministry of Economic Affairs Printed by the Intellectual Property Bureau employee consumer cooperative 491941 A7 B7 V. Description of the invention (7) Cross-reference table for printed components of the Intellectual Property Bureau employee consumer cooperative of the Ministry of Economic Affairs 100 Micro-Dip Disk 102 Analysis Hole 104 Side Wall 106 Substrate 108 Bottom 200 Template 202 Material Source 204 lower stencil impression 206 upper stencil impression 208 metal master 210 stencil cavity 300 analysis flask 302 sample tray 304 sample surface 306 bottom surface 308 three-dimensional surface structure 310 housing 312 slot 314 plug 400 analysis device 402 tray 404 channel 406 Top surface (please read the precautions on the back before filling this page) 4 items to refill, assemble and order # This paper size applies to China National Standard (CNS) A4 specification (210X297 mm) _ 1〇_ 491941 A7 B7 5 Description of the invention (8) 408 bottom surface 410 inner wall 412 three-dimensional surface structure 414 cover 416 bottom portion 500 analysis device 502 light source 504 detector 5 06 Processor 508 Detailed description of the preferred embodiment of the light distribution 1. An analysis device containing a three-dimensional structure A. Microdroplet disk In view of the above, and referring to Figures 1 and 2, a microdroplet disk 100 contains most analysis holes Slot 1 0 2. Each analysis hole slot 102 has a generally cylindrical sidewall 104 extending over a substantially planar substrate 106 to define a bottom 108. The micro-volume disc 100 is preferably formed of a plastic material, such as polystyrene strip, polycarbonate, polyvinyl chloride (PVC), etc., which is selected in view of optical transmission properties. The microtiter disk 100 may be formed to include a plurality of analysis wells 102, of which six analysis wells are shown in Fig. 1 to facilitate the description of the present invention. Although each analysis well slot 102 is shown as having a cylindrical shape, it should be understood that other shapes may be used without departing from the general scope of the invention. For example, referring to Figure 3-5, it shows several alternative configurations of analysis slots. Individually marked paper size is applicable to Chinese national standard (0 milk) 8 4 specifications (210, 乂 297 mm) (please read the note on the back first) Please fill in this page again for the matters) Order 491941 A7 ____ B7 printed by the Intellectual Property Bureau of the Ministry of Economic Affairs. V. Invention Description (9) 102 ', 102' ', 102' ''. As shown in Fig. 3, the analysis well groove 102 'has a circular column shape. On the other hand, each analysis slot 102 '' may have a prismatic shape (i.e., a rectangular shape) to increase the slot density. Similarly, the analysis hole groove 102 '' shown in Fig. 4 has a circular prism shape with corners having a radius "r". Of course, if you want to further increase the density of holes, you can use prism-shaped holes with square corners. Fig. 5 shows an analysis slot 102 ... having a non-normal cylindrical structure. That is, the bottom 108... Does not form a right angle with the side wall 104. Conversely, the analysis slot 10 2... Has a bottom portion 108, which is generally “tulip” or parabolic. It should be understood that several additional shapes include "U" bottoms, "V" bottoms, and others that can be used to increase cell density, increase surface area, increase capacity, etc., to meet the needs of a particular analytical application. Referring again to FIG. 2, each bottom 108 is formed to include a three-dimensional structure that is configured to enhance the optical ' B. Manufacture of three-dimensional structures has been found to have a three-dimensional structure using diffuser technology, such as in US Patent Nos. 5,3 6 5,3 5 4, 5,5 3 4,3 8 6 and 5,609,93 9 The disclosed diffuser structure, which is specifically incorporated herein for reference, provides a * environment that is unexpectedly beneficial to the sample. As described therein, a three-dimensional photoforming structure that includes random, chaotic, rather than flat, smears can be recorded and developed in a photosensitive medium such that the refractive index (eg, the gradient refractive index) of the medium has no An intermittent and smooth change that scatters collimated light into a pattern with smooth brightness control. The three-dimensional surface structure formed in this way can be reproduced with any number of materials, transparent or ^ 's scale applicable to Chinese National Standard (CNS) A4 specifications (210X297 mm ·)-I, (Please read the precautions on the back first (Fill in this page again) Binding · Printed by the Consumers 'Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs 491941 Printed by the Consumers' Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (10) Non-transparent, and then adapted to form the bottom 1 08 . More specifically, a coherent (co-hern) laser light coupled through an objective lens can be used to record the three-dimensional surface structure of an autonomous diffuser into a photosensitive medium in the future. The main diffuser can be a standard ground glass diffuser, a lenticular diffuser, an acetate diffuser, or a hologram diffuser. The size, shape, and orientation of surface features recorded in photosensitive media are a function of several variables, which include the type of objective lens and main diffuser used, and the relative positions of those components relative to each other and relative to the photosensitive media . Finally, the desired results are obtained through empirical tests, and in particular, the three-dimensional structure that is most suitable for adhering and / or cultivating a particular sample will be achieved through these tests. General statements about the effects on the formation parameters of the three-dimensional surface structure can be stated and will facilitate the selection of a configuration to obtain the desired three-dimensional surface structure. The objective lens expands the coherent light so that the area of the light coming from the objective lens and incident on the main diffuser is larger than the cross-sectional area of the laser beam itself. The small magnification of the objective lens results in a small incident on the main diffuser. In this case, the characteristics recorded on the photosensitive medium are large. Therefore, the size of the aperture of the light incident on the main diffuser is inversely related to the size of its surface features recorded in the photosensitive medium. The distance between the objective and the main diffuser also affects the size of the surface structure. As the distance between the objective lens and the main diffuser decreases, the size of the surface structure increases. This happens because when the objective lens moves closer to the main diffuser, the appearance gap of the light incident on the main diffuser becomes smaller. The increased structure size provides a three-dimensional surface structure with reduced optical diffusion, i.e., a smaller angular light distribution. Conversely, if it is in the standard of the physical paper, the national standard (CNS) A4 specification (210X297 mm). 13-丨 Order I · (Please read the precautions on the back before filling this page) 491941 A7 _ B7 V. Description of the invention (11) As the distance between the mirror and the main diffuser increases, the size of the surface structure decreases. The smaller surface structure causes a larger angular dispersion. (Please read the precautions on the back first (Fill in this page again) The distance between the main diffuser and the photosensitive medium also affects the size of the smear. When the distance decreases, the size of the smear recorded in the photosensitive medium also decreases. This happens because Assume that one of the enlarged beams of light is generated on the objective lens. When the photosensitive medium is moved closer to the main diffuser, the light beam emitted by each irregularity in the autonomous diffuser will reach the photosensitive medium. Previously it was enlarged less, resulting in smaller smears. Conversely, if the distance between the main diffuser and the photosensitive medium is increased, the size of the recorded smudges will increase. Therefore, these media In objective lens, main diffuse The simple relationship between the emitter and the photosensitive medium is adjusted (empirically) to obtain the desired smear size in the photosensitive medium, which thus determines the light output distribution of the diffuser generated from the photosensitive medium, such as This is further explained below. The printing of photosensitive media by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs can also be recorded by using other methods (including irrelevant light sources and contact printing processing procedures), such as applied for on August 20, 1998. US patent applications under review No. 09/1 37,3 9 7 (of which CPA applications were filed on February 29, 2000) and US additional patent applications filed on May 8, 1998 07 5,023 (now abandoned), these disclosures are specially incorporated here for reference. An optimal three-dimensional surface structure with an ideal light distribution pattern was generated using the above processing procedure and used to Produce a durable metal master with the ideal three-dimensional surface structure modified using standard electroplating techniques. More specifically, the three-dimensional surface structure is recorded in a photosensitive medium, which is then grown. From Photosensitive media is made of a copy structure. It is best to apply the Chinese National Standard (CNS) A4 specification (210X297 mm) by using a hard paper size. 14 _ 491941 Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Explanation (12) A layer of epoxide is formed on the three-dimensional surface structure of the photosensitive material. The hardened epoxide can be removed from the photosensitive material and leave a three-dimensional surface structure recorded in it. A thin layer of silver metal is adhered to Deposited and metallized on hardened epoxide. A cathode is then coupled to silver and an anode is coupled to a nickel strip. The silver-coated epoxide and nickel strip are then immersed in an acid solution, and A current passes through the silver, the acid solution, and the nickel bars, causing a layer of nickel to form on the silver. This layer can grow to 0.5 inches thick. The epoxide, silver, and nickel structures are removed from the acid solution, and the epoxide and silver are removed from the nickel, leaving a metal master, that is, a nickel pad having a three-dimensional surface structure formed therein ( shm). Referring to FIG. 13, there is shown a micro-droplet stencil 200 including a metal master 208 formed in the manner described above. The template 200 includes a lower template stamp 204 and an upper template stamp 206. The metal master plate 20 8 is fixed to the lower stencil stamp 204 by welding, heating, forging, joining, or other appropriate methods, and requires careful operation so as not to damage the three-dimensional surface structure formed therein. A stencil cavity 210 is then formed in the lower stencil stamp 204, for example, manufactured by electric discharge machining (EDM). The stencil cavity defines a complement of the shape of the micro-droplet. As is known in the art, the upper stencil die 20 6 covers the stencil cavity 2 10 and the plastic material is injected from the source 202 into the stencil cavity. It should be understood that its stencil cavity 2 10 is formed with appropriate undulations and traction angles to help remove the completed microdroplet disk. In addition, various release mixtures can be used. C · Single piece, flask, and concentrator containing three-dimensional light-forming structure The above manufacturing method can be used to form a variety of paper containing micro-droplet discs. This paper size applies Chinese National Standard (CNS) A4 specifications (210X297):丨 ^ ^ Order. I. (Please read the notes on the back before filling out this page) 491941 A7 B7 Printed by the Intellectual Property Bureau Employees Consumer Cooperatives of the Ministry of Economic Affairs 5. Analysis device of the invention (13). The simplest such structure is a flat analysis disk, i.e. a monolith, which has a surface formed to contain a three-dimensional surface structure. 6 and 7, there is shown an analysis flask 300 constructed in accordance with a preferred embodiment of the present invention. The analysis flask 300 contains a sample plate 302. The sample tray 302 has a sample surface 304 and a bottom surface 306. The sample surface 304 is preferably formed to contain a three-dimensional surface structure 308 as described herein. The sample surface 3 0 4 is covered with an optically transparent housing 310 which seals the periphery of the sample tray 30 2. A slot 312 is formed in the housing 3.10, and a plug 314 is provided to allow introduction or sealing of the substance in the flask 300. Of course, where the housing 310 is used only for growing cells, it need not be transparent. Referring to FIG. 15, another analysis device 400 is formed in the disk 402. More specifically, the disc 402 is formed to include at least one channel 404 extending from a top surface 406 through the disc 402 to a bottom surface 408. The channel 404 defines an inner wall 4 10. As shown, the channel 404 has a tapered or "V" structure. It should be understood that other architectures can also be used. Meanwhile, the channel 404 may define an elongated slit in the disc 402, or may define a cylindrical or frustoconical slot. The inner wall 4 1 0 is formed to contain a three-dimensional surface structure 4 1 2. The sample substance can pass through the channel 4 04 and a part of the substance is retained in the three-dimensional surface structure 4 1 2 by the adhesive. In addition, a cover 414 may be provided to seal one bottom portion 416 of the channel 404. In this configuration, the channel 404 is configured as an analysis well with a sample substance retained therein. The cover 4 1 4 can then be removed to allow sample material to flow out of the channel 404. Therefore, several steps can be completed to infuse and flow out the sample or pass through the channel 404. The accumulated adherent sample material in the three-dimensional surface structure 412 can then be analyzed using several analytical techniques. This scale is applicable to the Chinese National Standard (CNS) A4 specification (210X297 mm) (Please read the precautions on the back before filling out this page) • Binding and ordering # 491941 Printed by the Consumers ’Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Description of the Invention (14) Referring to FIG. 8, another exemplary analysis device 500 is shown to analyze a sample prepared in a microtiter disk (for example, a microtiter disk 100). The device 500 includes a light source 502, a detector 504, and a processor 506. The light source 502 is configured to illuminate one or more of the analysis wells 100 of the micro-disc disc 100 with a coherent laser beam. According to a preferred embodiment of the present invention, the light distribution 508 from the illuminated analysis cell is optically shaped by the three-dimensional surface structure formed in the bottom 108. For the micro-disc disc 100, its light distribution is preferably a circular distribution with an angle Φ of about 1-10 degrees, and more ideally, for a CCD detector, it is about 2-3 degrees (see figure). Distribution shown in Figure 9 and the corresponding structure shown in Figure 10). By incorporating a three-dimensional surface structure in the bottom 108, substantially more incident light is directed to the detector 504, and less is wasted. The detector 504 is preferably a charge coupled device (CCD) configured to be coupled to the output of the processor 506 to process and analyze sample data. Another important advantage of using the microtiter disk of the invention or any other sample disk or chamber containing the three-dimensional surface structure of the invention is that it comes from the light source 502 and the light incident on the microtiter disk 100 does not backscatter. Light source 502. Instead, it is transmitted to the sample, where the light system interacts with the sample. This increases its interaction and the intensity of the light emitted from the sample. In any type of spectroscope system, it can be Raman (Raman) or any other type. It is not desirable to have light that deviates from the wavelength of the light source outside the sample, because this will reduce the 'signal-to-noise ratio of the system' and make it more difficult. Distinguish the desired light signal from the sample from the light from the light source. Especially in Raman beamsplitters, the paper size that is emitted from the sample with a slight deviation from the wavelength of the light source is applicable to the Chinese National Standard (CNS) A4 specification (210x297), -17- I ------ ---- r---IT! --―;-. 1 (Please read the precautions on the back before filling out this page) 491941 Printed by the Consumers ’Cooperative of Intellectual Property Bureau of the Ministry of Economic Affairs Α7 Β7 V. Description of Invention (15 ) The light intensity is low (compared to the intensity of the light source), making it difficult to detect the desired signal other than the source light. Low signal-to-noise ratios are therefore a problem in conventional systems for optical sample research. Because the analysis disk of the present invention significantly reduces backscatter, the analysis disk of the present invention can significantly improve the signal-to-noise ratio in most spectroscope systems. A micro-droplet disc formed according to the preferred embodiment is capable of transmitting more than 80 percent of incident light to the detector 504. On the other hand, the conventional micro-disc measuring disk can only transmit less than 10% of the incident light to the detector 504 because of its backscattering and uncontrollable diffusion. In addition, the broad distribution of light from the conventional microdroplet can cause cross talk on the detector 504. That is, the neighboring pixels of the CCD corresponding to the other analysis wells of the micro-droplet may be irradiated, causing an incorrect indication. With higher density microtiter disks, it becomes increasingly important to direct a higher percentage of incident light towards the detector in a controlled manner. As mentioned above, one of the advantages of forming a three-dimensional surface structure in this way is that its light distribution pattern from the diffuser can be controlled. This is the opposite of the traditional diffuser, which does not provide control of the output light distribution, but only provides Lambertian diffusion. Referring to Figures 9 to 12, a pair of light distribution patterns and a corresponding three-dimensional surface structure for generating the light distribution patterns are shown. More specifically, referring to FIG. 9, it shows a circular light distribution having an angular range Φ of 10 degrees, and more preferably, about 2-3 degrees. The corresponding structure is shown in the photo in FIG. In contrast, Figure 11 shows an elliptical light distribution pattern with a wider angle (φ equals about 2 degrees compared to φ equals about 80 degrees). The selection of an appropriate three-dimensional surface structure will depend on several factors, including the samples to be prepared. 'Analysis of this paper size applies Chinese National Standard (CNS) A4 specification (210X297 mm)-化 _ 1 ------ IT- ---- 1--Ί (Please read the notes on the back before filling out this page) 491941 Printed by the Consumer Cooperative of the Intellectual Property Bureau of the Ministry of Economic Affairs A7 B7 V. Invention Description (16) Technology and analysis equipment Detector size and type. Two important characteristics of the three-dimensional surface structure are: the aspect ratio, that is, the ratio of the depth of the surface structure to the height; and the vertex ratio ', the ratio of the depth of the surface structure to the diameter. The dimensional characteristics of the surface structure are illustrated as a circular structure in FIG. 14A and an elliptical structure in FIG. 14B. For example, an aspect ratio in the range of 1: 1: 1 to 1: 6 (depth to height), and a vertex ratio in the range of 1: 0.5 to 1: 1 (depth to diameter) can be used. Certain aspect ratios and vertex ratios should be selected according to their application. For example, the aspect ratio and vertex ratio of several structures are described in Table 1. It should be understood that its three-dimensional structure is completely random, and therefore, a solid measurement is not possible. Therefore, the better aspect ratio and vertex ratio of a certain structure are predicted by measuring several structures. It should be understood that other larger or smaller aspect ratios and vertex ratios may be used, but current manufacturing limitations may limit larger aspect ratios. A preferred configuration can utilize a surface structure of about 1 to 5 microns high and a width of about 20 microns to the cell, and a smaller surface structure to the molecule. Both larger and smaller structures can be used. In either case, the optimal surface structure is based on the sample, and the aspect ratio is selected to obtain the maximum surface area of the relevant sample. Such control and optimization of the surface structure and surface area is therefore impossible. In addition, three-dimensional surface structures can be formed with a substantially random orientation that facilitates sample growth. (Please read the notes on the back before filling out this page)
、1T 漫射器 深度 商度 寬度 縱橫比 頂點比 型式 (# m ) (β m) (β m) 110°圓形 8 20 1: 2.5 1： 0.82 60°圓形 5 10 1:2 1: 0.90 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐） 19- 491941 A7 B7 五、發明説明（17) 30°圓形 80°圓形 95° X 25° 橢圓 16 12 27 6.5 1: 5.4 0.60 0.95 0.77 表I ··光成形結構之尺寸特性 經濟部智慧財產局員工消費合作社印製 2. 包含三維結構之裝置的使用 A.聚集器 由於漫射器結構之黏附性質，故其亦可被使用爲化學 物之聚集器。例如，許多試驗係檢測微量之一種化學物° 因此，欲達成一種方法以於檢測前首先聚集樣本。 聚集可.被執行以漫射器結構本身。此外，樣本可被聚 集（1 )以一種規律的、封閉的結構，例如圖1、3、及6中 所顯示者，或者（2 )以一種具有至少一通道之結構，如圖 1 5中所顯示者。於一種規律的、封閉的結構中，樣本被加 入孔槽或其他適當的裝置中，例如燒瓶，而樣本中之相關 化學物被容許黏附至漫射器結構。樣本液體被接著移除且 置換以新的樣本。再次，相關化學物被容許黏附，而接著 樣本液體被移除。此樣本之應用、黏附、及吸引被重複以 所欲的次數。接下來，相關化學物可被檢測，假如需要日寺 。此外，相關化學物可被量化。另一方面，假如不欲檢測 ，但僅欲聚集，則此方法終止。 使用漫射§&爲聚集§&於一種具通道之裝置時，樣φ j系 連續地或間斷地通過結構。通道可接著被封閉以加λ新白勺 液體，新的液體係包含更多樣本之多數液體。另—$胃5 (請先閲讀背面之注意事 4 項再填· 裝— - :寫本頁) -口 i- 491941 A7 ____ B7 五、發明説明（I8) 新的液體可爲染料或其他可被用以檢測黏附之化學物的化 學物。另一種方式係無檢測發生。反之，新的液體可含有 來自某一議定之試劑，其被加入聚集的化學物。此外，聚 集及其本身可爲所欲的終點。 漫射器結構亦可被欲塗敷以化學物。再次，規律的結 構或者含有通道之結構可被使用。本發明之許多改變及修 飾均可被實施而不會背離其一般的範圍及精神。某些改變 之範圍被討論如上。其他的範圍將從後附之申請專利範圍 可淸楚瞭解。 (請先閱讀背面之注意事項再填寫本頁) 經濟部智慧財產局員工消費合作社印製 -21 - 本紙張尺度適用中國國家標準（CNS ) A4規格（210X297公釐.)、 1T diffuser depth quotient width aspect ratio vertex ratio type (# m) (β m) (β m) 110 ° circular 8 20 1: 2.5 1: 0.82 60 ° circular 5 10 1: 2 1: 0.90 This paper size applies to Chinese National Standard (CNS) A4 (210X297 mm) 19-491941 A7 B7 V. Description of the invention (17) 30 ° round 80 ° round 95 ° X 25 ° oval 16 12 27 6.5 1: 5.4 0.60 0.95 0.77 Table I · Dimensional characteristics of light-formed structures Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs 2. Use of devices containing three-dimensional structures A. Due to the adhesive nature of the diffuser structure, the aggregator can also be used Used as a chemical concentrator. For example, many tests detect a trace amount of a chemical. Therefore, it is desirable to achieve a method in which a sample is collected before detection. Gathering can be performed with the diffuser structure itself. In addition, samples can be gathered (1) in a regular, closed structure, such as shown in Figures 1, 3, and 6, or (2) in a structure with at least one channel, as shown in Figure 15 Show by. In a regular, closed structure, the sample is added to a well or other suitable device, such as a flask, and related chemicals in the sample are allowed to adhere to the diffuser structure. The sample liquid is then removed and replaced with a new sample. Again, the relevant chemicals are allowed to adhere, and then the sample liquid is removed. The application, adhesion, and attraction of this sample were repeated as many times as desired. Next, relevant chemicals can be detected if Risi is needed. In addition, related chemicals can be quantified. On the other hand, if you do not want to detect but only want to aggregate, this method is terminated. When diffuse § & is used to gather § & in a channeled device, the sample φ j passes through the structure continuously or intermittently. The channel can then be closed to add a new liquid, the new liquid system containing most of the liquid for the larger sample. Another— $ stomach 5 (please read 4 notes on the back before filling and filling—-: write this page)-mouth i- 491941 A7 ____ B7 V. Description of the invention (I8) The new liquid can be dye or other A chemical used to detect adherent chemicals. The other way is that no detection occurs. Conversely, new liquids may contain reagents from an agreement that are added to aggregated chemicals. In addition, aggregation and itself can be the desired end point. The diffuser structure can also be coated with chemicals. Again, regular structures or structures containing channels can be used. Many changes and modifications of the present invention can be implemented without departing from its general scope and spirit. The scope of some changes is discussed above. Other scopes will be clearly understood from the attached patent application scope. (Please read the notes on the back before filling out this page) Printed by the Consumer Cooperatives of the Intellectual Property Bureau of the Ministry of Economic Affairs -21-This paper size applies to China National Standard (CNS) A4 (210X297 mm.)