CN2887882Y - Culturing and cleaning dish special for cell growing on cover glass - Google Patents
Culturing and cleaning dish special for cell growing on cover glass Download PDFInfo
- Publication number
- CN2887882Y CN2887882Y CN 200520079192 CN200520079192U CN2887882Y CN 2887882 Y CN2887882 Y CN 2887882Y CN 200520079192 CN200520079192 CN 200520079192 CN 200520079192 U CN200520079192 U CN 200520079192U CN 2887882 Y CN2887882 Y CN 2887882Y
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- CN
- China
- Prior art keywords
- cell
- cell climbing
- climbing sheet
- groove
- creep plate
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- Expired - Fee Related
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Abstract
The utility model discloses a cultivating and lavation utensil especially for cell climbing piece, consisting of a cultivating utensil, an edge climbing piece flute, a central climbing piece flute, a side inclined opening, a cell climbing piece, and a cultivating utensil cover. During the cell climbing making and treating procedure, the utility model guarantees the single-face cell on the cell climbing piece sticks to the wall and avoids the overlapping and friction between the climbing pieces, which makes the making and treating procedure of the cell climbing piece clean, fast, and safe, thus the quality of the cell climbing piece is increased. The utility model has a good effect and achieves the design requirements through a plurality of tests and probations,.
Description
Technical field
The utility model relates to the experimental article in a kind of biology, the basic medical research, and particularly a kind of cell climbing sheet is special-purpose to be cultivated and the washing ware.
Background technology
It is directly cell climbing sheet (little Cover Glass) to be placed common culture dish that traditional cell climbing sheet is cultivated, and the nutrient solution that will contain cell adds wherein, cultivates through certain hour, and cell attachment is made traditional cell climbing sheet on creep plate.Because traditional culture dish bottom surface is smooth smooth, closely paste mutually with cell climbing sheet, creep plate can be soaked with nutrient solution after adding cell suspension between culture dish, cell is also grown under cell climbing sheet simultaneously, cause traditional cell climbing sheet above and below that the problem of cell is all arranged, make troubles to handling creep plate in the future.
The washing of the cell climbing sheet for preparing generally is to carry out in common culture dish, if the washing simultaneously in a common culture dish of a plurality of cell climbing sheet usually can stack mutual friction, is easy to damaging cells, destroys the quality of cell climbing sheet.
Therefore, need special-purpose cultivation of a kind of cell climbing sheet of development and washing ware, overcome the above problems, make the preparation of cell climbing sheet and handle cleaner, convenient, safety, improve the quality of cell climbing sheet greatly.
Through the literature search that the applicant carries out, do not see device with similar similar technique of the present utility model.
Summary of the invention
The purpose of this utility model is, provides a kind of special use for preparing cell climbing sheet to cultivate and the washing ware, to solve the problem that easily causes its damage when all there are cell and washed cell creep plate in the two sides in the preparation cell climbing sheet in biology, the preclinical medicine experimental study.
The technical solution that realizes above-mentioned purpose is that a kind of cell climbing sheet is special-purpose to be cultivated and the washing ware.It comprises culture dish, edge creep plate groove, central creep plate groove, skew back mouth, cell climbing sheet and culture dish lid.It is characterized in that, in culture dish, recessed circle and quadrate creep plate groove (edge groove and central groove) in the bottom surface arranged with the edge, groove is lower than bottom surface 2-4mm, the bottom of groove has linear or spot-like projections, and high about 1-2mm plays the effect that holds up cell climbing sheet, avoid the cell growth below the creep plate, avoided tightly combining (principle has water with between two blocks of glass, is difficult to separately) simultaneously, helped the gripping of creep plate with the bottom.The design of groove is in order to separate each cell climbing sheet, to avoid overlapping each other and rubbing in cultivating and washing.Special-purpose culture dish has transparent plastics or glass to make.
Some other characteristics of the present utility model are, edge groove and central groove both sides are provided with the skew back mouth, at the bottom of the ware to the little inclined-plane of creep plate bottom land, wide about 5-8 millimeter is convenient to tweezers gripping cell climbing sheet.
The utility model, cell climbing sheet is special-purpose to be cultivated and the washing ware, owing to adopted creep plate groove at cell climbing sheet preparation and processing, guaranteed cell climbing sheet single face cell attachment, avoided the overlapping and friction between creep plate, make the preparation of cell climbing sheet and handle cleaner, convenient, safety, improve the quality of cell climbing sheet greatly.
Description of drawings
Fig. 1 is the special-purpose plan structure figure that cultivates and wash ware of cell climbing sheet of the present utility model; Symbol is respectively among the figure: A, culture dish, B, edge creep plate groove, C, skew back mouth, D, central creep plate groove, E, cell climbing sheet;
Fig. 2 is that structure iron is seen in the special-purpose side of cultivating and washing ware of cell climbing sheet of the present utility model;
Fig. 3 is the special-purpose local amplification assumption diagram of creep plate groove of cultivating and wash ware of cell climbing sheet of the present utility model; Top is vertical view, and following is that figure is seen in the side;
Below in conjunction with accompanying drawing and using method the utility model is further described.
Embodiment
Cell climbing sheet is special-purpose to be cultivated and the washing ware, forms (Fig. 1,2,3) by culture dish A, edge creep plate groove B, skew back mouth C, central creep plate groove D, cell climbing sheet E and culture dish lid F six parts.
It is characterized in that, in culture dish, recessed circle and quadrate creep plate groove (edge groove and central groove) in the bottom surface arranged with the edge, groove is lower than bottom surface 2-4 millimeter, the bottom of groove has linear or spot-like projections, and high about 1-2 millimeter plays the effect that holds up cell climbing sheet, avoid the cell growth below the creep plate, avoided tightly combining (principle has water with between two blocks of glass, is difficult to separately) simultaneously, helped the gripping of creep plate with the bottom.The design of groove is in order to separate each cell climbing sheet, to avoid overlapping each other and rubbing in cultivating and washing.Special-purpose culture dish can be made by multiple materials such as polystyrene, plastics, synthetic glass, glass.
Follow these steps to carry out in actual use:
1.. A sets level with culture dish, with tweezers cell climbing sheet E is clipped among edge creep plate groove B or the central creep plate groove D, frame in the bottom of groove have linear or spot-like projections on;
2.. in washing ware A, pour cell suspension into, carry out cell cultures behind the lid culture dish lid F;
3.. after cell climbing sheet is made, discard nutrient solution, pour washings into, will cultivate A and place the jolting at a slow speed of dull and stereotyped shaking table, fixed relative to one another between the cell climbing sheet D, being in the suds can not interact;
4.. washing finishes, and takes suction pipe to discard washings,, easily it is taken out from square groove B below skew back mouth C is inserted into cell climbing sheet D with tweezers;
5.. special-purpose cultivation of cell climbing sheet and washing ware can also use as the wet box of cell climbing sheet pathological staining, add entry in central creep plate groove D, play moisture preserving function; Frame carries out hatching and dyeing of cell climbing sheet E on the spot-like projections of the bottom of groove in edge creep plate groove B, and staining reagent can not flow out creep plate.After covering culture dish lid F, hatch for a long time and dye.
Claims (3)
1. cell climbing sheet is special-purpose cultivates and the washing ware, it comprises culture dish, edge creep plate groove, central creep plate groove, skew back mouth, cell climbing sheet and culture dish lid, it is characterized in that recessed circle and quadrate edge and central creep plate groove in the bottom surface being arranged in culture dish central authorities and edge; Ware body and ware lid can be made by polystyrene, plastics, synthetic glass, the multiple material of glass.
2. cell climbing sheet as claimed in claim 1 is special-purpose to be cultivated and the washing ware, it is characterized in that, and edge and central creep plate groove, groove is lower than bottom surface 2-4 millimeter, and the bottom of groove has linear or spot-like projections, high 1-2 millimeter.
3. cell climbing sheet as claimed in claim 1 is special-purpose to be cultivated and the washing ware, it is characterized in that, and the skew back mouth, for arriving the little inclined-plane of creep plate bottom land at the bottom of the ware, wide 5-8 millimeter is convenient to tweezers gripping cell climbing sheet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520079192 CN2887882Y (en) | 2005-08-04 | 2005-08-04 | Culturing and cleaning dish special for cell growing on cover glass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520079192 CN2887882Y (en) | 2005-08-04 | 2005-08-04 | Culturing and cleaning dish special for cell growing on cover glass |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2887882Y true CN2887882Y (en) | 2007-04-11 |
Family
ID=38046456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520079192 Expired - Fee Related CN2887882Y (en) | 2005-08-04 | 2005-08-04 | Culturing and cleaning dish special for cell growing on cover glass |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2887882Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445783B (en) * | 2008-12-31 | 2011-04-20 | 安徽省立医院 | Simple cell climbing film culture and experimental apparatus |
| CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
| CN106754368A (en) * | 2016-12-15 | 2017-05-31 | 广州洁特生物过滤股份有限公司 | A kind of manufacture method of cell culture apparatus |
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
-
2005
- 2005-08-04 CN CN 200520079192 patent/CN2887882Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445783B (en) * | 2008-12-31 | 2011-04-20 | 安徽省立医院 | Simple cell climbing film culture and experimental apparatus |
| CN103773683A (en) * | 2012-10-17 | 2014-05-07 | 安徽农业大学 | Layered-type cell culture plate |
| CN103773683B (en) * | 2012-10-17 | 2015-11-25 | 安徽农业大学 | Layer-stepping Tissue Culture Plate |
| CN106754368A (en) * | 2016-12-15 | 2017-05-31 | 广州洁特生物过滤股份有限公司 | A kind of manufacture method of cell culture apparatus |
| CN106754368B (en) * | 2016-12-15 | 2019-07-23 | 广州洁特生物过滤股份有限公司 | A kind of manufacturing method of cell culture apparatus |
| CN112094745A (en) * | 2020-09-09 | 2020-12-18 | 江苏益玛生物科技有限公司 | Processing method of high-stability surface biological cell culture dish |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |