CN215162539U - Instant anaerobic bag for anaerobic bacteria culture - Google Patents

Abstract

The utility model discloses a ready-to-use anaerobic bag that can be used to anaerobe to cultivate, its technical scheme main points are: including the bag body, fixed first sealing of sack department and the second sealing, the fixed introduction port of having seted up in intermediate position of the bag body, the fixed connecting band that is provided with in wall limit of introduction port, connecting band fixedly connected with chock plug, the below of chock plug and the inner wall fixedly connected with rubber buffer of introduction port, the utility model discloses storage and operation process both can not reveal sample and smell, also do not have external air infiltration, guaranteed experimental apparatus's leakproofness, ensured experimental result's accuracy, the device reaches anaerobic environment's effect through the oxygen in the direct absorption air, need not to use the high-pressure nitrogen gas source, not only economic saving but also safety and convenience, do not need the experimenter to possess certain anaerobism experiment operation experience and laboratory safety knowledge, easy operation maintains the convenience.

Description

Instant anaerobic bag for anaerobic bacteria culture

Technical Field

The utility model relates to the technical field of microorganisms, in particular to a ready-to-use anaerobic bag for anaerobic bacteria culture.

Background

Although large-area microbial infections have been reduced year by year with the improvement of sanitary conditions in recent years, microbial infections still account for a large portion of infectious diseases, among which anaerobic bacteria can cause infections of various parts of the human body, including appendicitis, cholecystitis, otitis media, oral infections, endocarditis, endometritis, brain abscess, myocardial necrosis, osteomyelitis, peritonitis, empyema, salpingitis, septic arthritis, liver abscess, sinusitis, wound infections after intestinal surgery or trauma, pelvic inflammation, bacteremia, etc., anaerobic bacteria are a type of bacteria that grow better under anaerobic conditions than in aerobic environments and cannot grow on the surface of solid media with oxygen content of more than 18% in air and/or carbon dioxide concentration of less than 10%, anaerobic bacteria lack an intact metabolic enzyme system, and the energy metabolism thereof proceeds in an anaerobic fermentation manner, if the anaerobic bacteria are exposed in the air for about 10 minutes, the culture positive rate of the anaerobic bacteria is influenced, and the anaerobic bacteria need to be cultured when the anaerobic bacteria infection is diagnosed.

However, the anaerobic culture requires special instruments and operation tables, and an anaerobic operation box is generally used as a simple substitute for the special instruments, but has the problems of poor sealing performance, severe consumption when air is replaced by nitrogen, difficulty in reaching strict anaerobic conditions, and the like, so that the experimental results are not ideal. The simple and efficient maintenance-free anaerobic operation bag (CN201820128731.8) solves the problems to a certain extent, but the device is still inconvenient to use, and experimental personnel are required to have certain anaerobic experiment operation experience and laboratory safety knowledge, particularly a high-pressure nitrogen source required by experiments, so that the device has higher requirements on operation safety.

SUMMERY OF THE UTILITY MODEL

To the problem mentioned in the background art, the utility model aims at providing a can be used to the instant type anaerobism bag that anaerobe was cultivateed to solve the sealed effect of the device that mentions in the background art unsatisfactory and to the problem that the experimenter operation required height.

The utility model discloses a realize through following technical scheme:

the utility model provides a can be used to instant type anaerobism bag that anaerobe was cultivateed, comprises a bag bod, the sack has been fixed to one side of the bag body, the fixed first banding that is provided with in sack department, the fixed second banding that is provided with in below of first banding, the fixed introduction port that has seted up in intermediate position of the bag body, the fixed connecting band that is provided with in wall limit of introduction port, connecting band fixedly connected with chock plug, the below of chock plug and the inner wall fixedly connected with rubber buffer of introduction port, fixed first bearing area and the second bearing area of being provided with on the inner wall of the bag body, the intermediate position activity of the internal of bag is provided with the flat board.

Furthermore, both ends of the bag opening are fixedly provided with buckles.

Through adopting above-mentioned technical scheme, bag body junction is more fragile, prevents when opening the sack, and the too big damage bag body of exerting oneself plays the guard action.

Furthermore, the chock plug is in a round table shape, is narrow at the top and wide at the bottom, and has the characteristics of viscosity, elasticity and water resistance.

By adopting the technical scheme, the circular truncated cone has the characteristics of convenient use, good sealing effect, viscosity, elasticity and water resistance, and the tightness of the device is further enhanced.

Furthermore, the first bearing area and the second bearing area are made of air-permeable material sterile non-woven fabrics.

Through adopting above-mentioned technical scheme, aseptic non-woven fabrics is aseptic ventilative material, can not influence the experiment effect, can also provide a good reaction environment.

Further, an oxygen indicator strip is movably arranged in the bag body.

By adopting the technical scheme, whether the anaerobic condition required by the experiment is met can be visually seen, and the indicator strip is the Resazurin with the concentration of one thousandth.

Furthermore, the bag body is made of transparent and unbreakable PA and PE composite plastic.

Through adopting above-mentioned technical scheme, the follow-up observation of shooing of being convenient for of transparent material is difficult damaged the use number of times that has improved the device, has further reduced the experiment cost.

To sum up, the utility model discloses mainly have following beneficial effect:

the utility model provides a can be used to anaerobe and cultivate instant anaerobic bag, the fixed first seal that is provided with of sack department seals with the second, the fixed connecting band that is provided with in wall limit of introduction port, connecting band fixedly connected with chock plug, the below of chock plug and the inner wall fixedly connected with rubber of introduction port, make storage and operation process both can not reveal sample and smell, also can not have external air infiltration, experimental apparatus's leakproofness has been guaranteed, experimental result's accuracy has been ensured, the device reaches anaerobic environment's effect through the oxygen in the direct absorption air, need not to use the high-pressure nitrogen gas source, not only economic saving but also safety and convenience, it possesses certain anaerobism experiment operation experience and laboratory safety knowledge not need the experimenter, easy operation maintains conveniently.

Drawings

The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:

fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a schematic view of a sample inlet structure.

In the figure: 1. a bag body; 2. a bag opening; 201. buckling; 3. a first sealing tape; 4. a second sealing tape; 5. a sample inlet; 501. a connecting belt; 502. a plug head; 503. a rubber plug; 6. a first bearing area; 7. a second bearing area; 8. a flat plate; 9. an indicator strip.

Detailed Description

In order to make the objects and technical solutions of the present invention clear and fully described, and the advantages thereof more clearly understood, the embodiments of the present invention are described in further detail below with reference to the accompanying drawings.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "inner", "outer", "top", "bottom", "side", "vertical", "horizontal", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," "fourth," "fifth," and "sixth" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Example 1

Referring to fig. 1-2, comprising a bag body 1, a bag opening 2 is fixedly arranged at one side of the bag body 1, a first sealing strip 3 is fixedly arranged at the bag opening 2, a second sealing strip 4 is fixedly arranged below the first sealing strip 3, a sample inlet 5 is fixedly arranged at the middle position of the bag body 1, a connecting strip 501 is fixedly arranged at the wall edge of the sample inlet 5, a plug 502 is fixedly connected with the connecting strip 501, a rubber plug 503 is fixedly connected with the lower part of the plug 502 and the inner wall of the sample inlet 5, a first bearing area 6 and a second bearing area 7 are fixedly arranged on the inner wall of the bag body 1, a flat plate 8 is movably arranged at the middle position in the bag body 1, the utility model discloses a carbon dioxide generating compound is put in the first bearing area 6, a high efficiency reducing agent is put in the second bearing area 7, anaerobic condition can be simply and conveniently formed in a culture bag, is favorable for growth of anaerobic bacteria, and can be put in a refrigerator for standby at any time, with the double functions of low temperature and oxygen absorption, the method can meet the more rigorous experimental requirements.

Referring to fig. 1, both ends of the bag opening 2 are fixedly provided with buckles 201, the connection of the bag body 1 is fragile, and the bag body 1 is prevented from being damaged by excessive force when the bag opening 2 is opened, so that the protective effect is achieved.

Referring to fig. 2, the plug 502 is in a truncated cone shape, has narrow top and wide bottom, has the characteristics of viscosity, elasticity and water resistance, is convenient to use, has good sealing effect, and further enhances the tightness of the device.

Referring to fig. 1, the first bearing area 6 and the second bearing area are made of 7 aseptic non-woven fabrics made of air-permeable materials, and the aseptic non-woven fabrics are made of aseptic air-permeable materials, so that the experimental effect is not influenced, and a good reaction environment can be provided.

Referring to fig. 1, an oxygen indicator strip 9 is movably arranged in the bag body 1, so that whether the oxygen-free condition required by the experiment is met or not can be visually seen, and the indicator strip 9 is resazurin with one thousandth of concentration.

Referring to fig. 1, the bag body 1 is made of a transparent and difficult-to-damage PA and PE composite plastic material, the transparent material is convenient for subsequent photographing and observation, the damage is difficult, the use times of the device are increased, and the experiment cost is further reduced.

To sum up, the utility model is a ready-to-use anaerobic bag for anaerobic bacteria cultivation, when in use, a plate 8 coated with target anaerobic bacteria and a contrast group plate 8 are respectively placed in a sterile anaerobic bacteria cultivation bag, a first sealing belt 3 and a second sealing belt 4 are closed, a plug 502 is opened, an injector is used to penetrate a rubber plug 503 to remove air in the cultivation bag, the plug 502 is closed quickly after the air is removed completely, so that the sample and smell can not be leaked in the storage and operation processes, and no external air infiltration can be generated, the tightness of the experimental device is ensured, the accuracy of the experimental result is ensured, the bag is kept still until an oxygen indicator strip 9 shows that the oxygen concentration is zero, the anaerobic bacteria cultivation bag provided with the plate 8 is placed in the cultivation environment required by anaerobic bacteria for cultivation, the whole anaerobic bag is both an operation bag and a cultivation bag, and can be directly placed in a common incubator for cultivation, need not worry the pollution of culture and reveal, the device reaches anaerobic environment's effect through the oxygen in the direct absorption air, need not to use high-pressure nitrogen gas source, and not only economy is practiced thrift but also safe convenient, does not need the experimenter to possess certain anaerobism experiment operation experience and laboratory safety knowledge, and easy operation maintains the convenience.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (6)

1. A ready-to-use anaerobic bag for anaerobic bacteria culture, which comprises a bag body (1) and is characterized in that: the fixed sack (2) of having seted up in one side of the bag body (1), sack (2) department is fixed to be provided with first sealing tape (3), the fixed second sealing tape (4) that is provided with in below of first sealing tape (3), introduction port (5) have been seted up to the intermediate position of the bag body (1), the fixed connecting band (501) that is provided with on wall limit of introduction port (5), connecting band (501) fixedly connected with chock plug (502), the below of chock plug (502) with the inner wall fixedly connected with rubber buffer (503) of introduction port (5), the fixed first bearing area (6) and the second bearing area (7) of being provided with on the inner wall of the bag body (1), the intermediate position activity in the bag body (1) is provided with flat board (8).

2. The ready-to-use anaerobic bag for anaerobic culture according to claim 1, wherein: both ends of the bag mouth (2) are fixedly provided with buckles (201).

3. The ready-to-use anaerobic bag for anaerobic culture according to claim 1, wherein: the plug head (502) is in a round table shape, is narrow at the top and wide at the bottom, and has the characteristics of double surfaces, elasticity and water resistance.

4. The ready-to-use anaerobic bag for anaerobic culture according to claim 1, wherein: the first bearing area (6) and the second bearing area (7) are made of air-permeable aseptic non-woven fabrics.

5. The ready-to-use anaerobic bag for anaerobic culture according to claim 1, wherein: an oxygen indicator strip (9) is movably arranged in the bag body (1).

6. The ready-to-use anaerobic bag for anaerobic culture according to claim 1, wherein: the bag body (1) is made of transparent PA and PE composite plastic materials which are not easy to damage.

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