CN206736275U - A kind of culture plant cell device - Google Patents

A kind of culture plant cell device Download PDF

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Publication number
CN206736275U
CN206736275U CN201720460694.6U CN201720460694U CN206736275U CN 206736275 U CN206736275 U CN 206736275U CN 201720460694 U CN201720460694 U CN 201720460694U CN 206736275 U CN206736275 U CN 206736275U
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CN
China
Prior art keywords
nutrient solution
storage unit
culture
culturing room
solution storage
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
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CN201720460694.6U
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Chinese (zh)
Inventor
王玲丽
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Yuncheng University
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Yuncheng University
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Priority to CN201720460694.6U priority Critical patent/CN206736275U/en
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Publication of CN206736275U publication Critical patent/CN206736275U/en
Expired - Fee Related legal-status Critical Current
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Abstract

The utility model discloses a kind of culture plant cell device, including culturing room, humidity controlling unit, nutrient solution storage unit, at least two liquid delivery lines and backboard;The bottom of nutrient solution storage unit is provided with an opening, opening is provided with the O-ring outside the culturing room, humidity controlling unit is located at the inside of culturing room with nutrient solution storage unit, and humidity controlling unit is located at the periphery of nutrient solution storage unit, opening is blocked in test piece when being placed in culturing room bottom, at least two liquid delivery lines connection nutrient solution stores unit to carry out the input of liquid and output;Test piece is provided with cell culture area, using change of the cell in test piece in incubation, by detection units such as quartz crystals, can real-time acquisition correlation analysis data, there is provided the detection mode of user more efficiently.

Description

A kind of culture plant cell device
Technical field
The utility model is related to cell culture apparatus technical field, particularly relate to it is a kind of can at-once monitor plant cell training Support device.
Background technology
At present, in general cell culture technology is using the method manually cultivated, and carries out large-scale culture cell.Cell The purpose of culture, in addition to obtaining a large amount of target cells in beneficial to the short time, another purpose, that is, it is to cultivate through observation The change of cell in journey, deduce each tissue characteristics in plant.
Because the characteristic of plant is different, therefore the species of cell culture apparatus is various, and purposes is also each different.According to The suitable cell culture apparatus of the exclusive characteristic design of various cells, and arrange in pairs or groups appropriate culture medium and culture environment, can be improved The efficiency of cultured in vitro.But existing cell culture apparatus is complicated, and there is not good real time monitoring function, to cell Culture causes certain inconvenience.
Therefore, it is necessary to a kind of new culture plant cell device is designed, to solve above-mentioned technical problem.
Utility model content
For problem present in background technology, the purpose of this utility model is to provide a kind of culture plant cell device, ,, can real-time by detection units such as quartz crystals using change of the cell in test piece in incubation with unification test piece Obtain correlation analysis data, there is provided the detection mode of user more efficiently.
What the technical solution of the utility model was realized in:A kind of culture plant cell device, with unification test piece, including Culturing room, humidity controlling unit, nutrient solution storage unit, at least two liquid delivery lines and backboard;Wherein, the culturing room Provided with multiple side walls, gas of the culturing room filled with culture cell, the bottom of nutrient solution storage unit is provided with an opening, described to open Mouth is provided with O-ring, and the O-ring is located at the outside of the culturing room, and the humidity controlling unit is located at nutrient solution storage unit The inside of the culturing room, and the humidity controlling unit is located at the periphery of nutrient solution storage unit, when test piece be placed in it is described The opening of nutrient solution storage unit, at least two liquid delivery lines connection nutrient solution storage unit are blocked during culturing room bottom To carry out the input of liquid and output;The test piece is provided with cell culture area, and the backboard is formed at cell culture apparatus One side.
In the above-mentioned technical solutions, the nutrient solution storage unit stores the nutrient solution for cultivating cell.
In the above-mentioned technical solutions, when the test piece is placed in culturing room bottom, the cell culture area in test piece Just in the open area of nutrient solution storage unit.
In the above-mentioned technical solutions, the gas is carbon dioxide.
In the above-mentioned technical solutions, the humidity controlling unit is provided with the cell body of at least one storage water.
In the above-mentioned technical solutions, the cell body forms concentric ring type around nutrient solution storage unit.
The utility model culture plant cell device, including culturing room, humidity controlling unit, nutrient solution storage unit, extremely Few two liquid delivery lines and backboard;The bottom of nutrient solution storage unit is provided with an opening, and opening, which is provided with, is located at the culturing room Outside O-ring, humidity controlling unit are located at the inside of culturing room with nutrient solution storage unit, and test piece is placed in culturing room bottom When block opening, at least two liquid delivery lines connection nutrient solution storage unit is to carry out the input of liquid and output;In test piece ,, can be real-time by detection units such as quartz crystals using change of the cell in test piece in incubation provided with cell culture area The acquisition correlation analysis data of property, there is provided the detection mode of user more efficiently.
Brief description of the drawings
Fig. 1 is the utility model three-dimensional exploded view;
Fig. 2 is the utility model schematic side view;
Fig. 3 is the utility model schematic cross-sectional view.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describe, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole realities Apply example.Based on the embodiment in the utility model, those of ordinary skill in the art institute under the premise of creative work is not made The every other embodiment obtained, belong to the scope of the utility model protection.
As shown in Figure 1 to Figure 3, a kind of culture plant cell device 100 described in the utility model, including one have it is more The culturing room 10 of individual side wall, the culturing room 10 can pass through the filling of gas before use, and the species of gas is visually intended to be trained Depending on foster cell, such as carbon dioxide.
In culturing room 10, nutrient solution storage unit 11 is further included, its bottom is provided with an opening.When the holding of test piece 20 When cell culture apparatus 100, nutrient solution storage unit 11 forms a space, beneficial to storing nutrient solution.Meanwhile when test piece 20 , can be more sandwiched wherein by the O-ring 30 outside culturing room 10 when being placed in cell culture apparatus 100, to increase nutrient solution storage The seal between unit 11 and test piece 20 is put, and the nutrient solution spill and leakage of input can be avoided, can also avoid the generation of pollution.
In addition, nutrient solution storage unit 11 further includes two liquid delivery lines 15,16, this two liquid delivery lines 15,16 It can connect inside extraneous and nutrient solution storage unit 11.Thus two liquid delivery lines 15,16, can be by the nutrient solution needed for cultivating Or other liquid are input in nutrient solution storage unit 11, and unwanted liquid is removed in good time.And this two liquid delivery line 15, 16 setting, more can be in the cell cultivation process for need lasting replacing nutrient solution, there is provided use, avoid cultivating because changing Liquid, and move into the continuous removal of culture apparatus and pollute.
In culturing room 10, humidity controlling unit 12 is located at nutrient solution storage unit 11 periphery, and this configuration can make humid control Unit 12 uniformly provides appropriate humidity and stores unit 11 to nutrient solution.It is suitable even in the nutrient solution needed for incubation It is micro, it will not also be reduced because of humidity, have influence on the moisture of nutrient solution indirectly, cause the concentration difference of whole nutrient solution.
In addition, be installed on to be suitable for the utility model cell culture apparatus 100 in a detection board (not shown), because This, cell culture apparatus 100 includes a backboard 40, is formed at a side of cell culture apparatus 100, and utilizes on backboard 40 At least a fixing hole 41 is fixed with board.
The test piece 20 for coordinating the utility model cell culture apparatus 100 to use, a cultivation region 21, this area are provided with its surface It is consistent with the nutrient solution storage bottom size of unit 11, after being placed in nutrient solution storage unit 11 bottom in favor of test piece 20, makes pre- The cell 22 first cultivated fully contacts with nutrient solution, is smoothed out cell culture.
With reference to the cell culture apparatus 100 after a test piece 20, the visible Fig. 2 of its side view.From Fig. 2 it can be seen that with reference to after The structure of cell culture apparatus 100, wherein, test piece 20 and nutrient solution storage unit 11 between can a sandwiched O-ring 30, make culture Liquid stores the space that unit 11 collectively forms nutrient solution storage unit 11 with test piece 20, can be used to retain a nutrient solution, for cell Use.
The cell 22 cultivated in advance in test piece 20, culture can be proceeded by after liquid delivery line 15,16 inputs nutrient solution. Meanwhile humidity controlling unit 12 forms an environment for being suitable to cell culture with the carbon dioxide in culturing room 10.
The structure of cell culture apparatus 100 is similar to the above embodiments in another embodiment, the difference is that humid control The form of unit 12, it is the humidity controlling unit 13 with multiple small cell bodies 131 in the present embodiment.Cell body 131 is around culture Liquid storage unit 11 forms concentric ring type.Humidity controlling unit 13 disclosed in this embodiment can also provide suitable in culturing room 10 When and uniform humidity.
The utility model culture plant cell device 100, including culturing room 10, humidity controlling unit 12, nutrient solution storage Unit 11, at least two liquid delivery lines 15,16 and backboard 40;The bottom of nutrient solution storage unit 11 is provided with an opening, opens Mouth is provided with the O-ring 30 outside the culturing room 10, and humidity controlling unit 12 is located at culturing room with nutrient solution storage unit 11 10 inside, and humidity controlling unit 12 is located at the periphery of nutrient solution storage unit 11, test piece 20 is placed in the bottom of culturing room 10 When block opening, at least two liquid delivery lines 15,16 connection nutrient solution storage unit 11 is to carry out the input of liquid and output; Test piece 20 is provided with cell culture area 21, using change of the cell in incubation in test piece 20, is examined by quartz crystal etc. Survey unit, can real-time acquisition correlation analysis data, there is provided the detection mode of user more efficiently.
Preferred embodiment of the present utility model is the foregoing is only, it is all at this not to limit the utility model Within the spirit and principle of utility model, any modification, equivalent substitution and improvements made etc., the utility model should be included in Protection domain within.

Claims (6)

  1. A kind of 1. culture plant cell device, with unification test piece, it is characterised in that:Including culturing room, humidity controlling unit, culture Liquid storage unit, at least two liquid delivery lines and backboard;Wherein, the culturing room is provided with multiple side walls, culturing room's filling There is the gas of culture cell, the bottom of nutrient solution storage unit is provided with an opening, and the opening is provided with O-ring, and the O-ring position In the outside of the culturing room, the humidity controlling unit is located at the inside of the culturing room with nutrient solution storage unit, and described Humidity controlling unit is located at the periphery of nutrient solution storage unit, and nutrient solution storage is blocked when test piece is placed in the culturing room bottom The opening of unit is put, at least two liquid delivery lines connection nutrient solution stores unit to carry out the input of liquid and output; The test piece is provided with cell culture area, and the backboard is formed at a side of cell culture apparatus.
  2. A kind of 2. culture plant cell device according to claim 1, it is characterised in that:The nutrient solution storage unit storage Put the nutrient solution for cultivating cell.
  3. A kind of 3. culture plant cell device according to claim 1, it is characterised in that:When the test piece is placed in culture During the bottom of room, the cell culture area in test piece is just in the open area of nutrient solution storage unit.
  4. A kind of 4. culture plant cell device according to claim 1, it is characterised in that:The gas is carbon dioxide.
  5. A kind of 5. culture plant cell device according to claim 1, it is characterised in that:The humidity controlling unit is provided with The cell body of at least one storage water.
  6. A kind of 6. culture plant cell device according to claim 5, it is characterised in that:The cell body is store around nutrient solution Put unit and form concentric ring type.
CN201720460694.6U 2017-04-28 2017-04-28 A kind of culture plant cell device Expired - Fee Related CN206736275U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720460694.6U CN206736275U (en) 2017-04-28 2017-04-28 A kind of culture plant cell device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720460694.6U CN206736275U (en) 2017-04-28 2017-04-28 A kind of culture plant cell device

Publications (1)

Publication Number Publication Date
CN206736275U true CN206736275U (en) 2017-12-12

Family

ID=60562965

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201720460694.6U Expired - Fee Related CN206736275U (en) 2017-04-28 2017-04-28 A kind of culture plant cell device

Country Status (1)

Country Link
CN (1) CN206736275U (en)

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CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20171212

Termination date: 20190428