CN218058989U - Portable cell hypoxia culture device - Google Patents
Portable cell hypoxia culture device Download PDFInfo
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- CN218058989U CN218058989U CN202221989298.XU CN202221989298U CN218058989U CN 218058989 U CN218058989 U CN 218058989U CN 202221989298 U CN202221989298 U CN 202221989298U CN 218058989 U CN218058989 U CN 218058989U
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Abstract
The utility model discloses a portable cell hypoxia culture device, which comprises a cell culture box; still include gaseous mixing device, connect by the mist output tube between gaseous mixing device and the cell culture box, be equipped with the gas pump on the mist output tube. The utility model provides a hypoxemia incubator is expensive, and is bulky be unfavorable for gaseous mixing, can expose in the cell transfer and the observation process shortcoming such as air. Can provide the hypoxic culture environment for the cell, inputed to the culture box after the gas mixture for gas environment is more even stable. The culture box is small in size, transparent, convenient to observe and transfer, and low in price, and can be placed in a common carbon dioxide incubator.
Description
Technical Field
The utility model belongs to the technical field of medical equipment and specifically relates to a portable cell hypoxia culture apparatus.
Background
For decades, animal cell culture has been aided only by CO2 concentration control, but new techniques and applications of cell therapy require that the environment in which the cells are cultured in vitro better mimic the conditions in vivo. The concentration of O2 in the organism is in the range of 1-14%, which is different from the O2 content in the atmosphere of 20-21%. Cells cultured under hypoxic conditions grow faster, have a longer lifespan, and exhibit lower stress responses. Double-airway or even three-airway incubators have appeared in the market of cell culture instruments and devices, but these devices are expensive (basically 10-40 ten thousand yuan), when observing the color of culture solution, observing the cell production condition and transferring cells to a biosafety cabinet for operation, a cell culture dish/bottle needs to be taken out and exposed in the air, the incubator is directly communicated with three air tubes, the flow rate of multi-path gas needs to be strictly controlled according to different gas concentrations after opening and closing a door, the uniform mixing is difficult, and the recovery of the gas concentration is not facilitated. For example, CN202021179042.3 is a multipurpose cell culture box, in which the concentration of gas in the box body is controlled by connecting two gas inlet pipes, and after the door is opened and closed, the flow rate of two gas inlet pipes needs to be regulated, so that it is difficult to achieve stable gas concentration in a short time.
Therefore, a need exists for a cell hypoxia culture device that is portable, easy to observe, and transfer, low in cost, capable of utilizing an existing carbon dioxide incubator, and capable of rapidly restoring gas concentration.
Publication (bulletin) no: CN203559058U, publication (announcement) date: 2014-04-23 relates to an intermittent type hypoxia cell culture device, including CO2 incubator and cavity, this cavity sets up in this CO2 incubator, the exit linkage of cavity and three-way valve, two entrances of this three-way valve all are connected with air supply device and nitrogen supply device respectively through switching control valve, the cavity pass through the hose and be connected with real-time oxygen concentration detection instrument, air supply device is the air pump, and nitrogen supply device is the nitrogen cylinder. The intermittent hypoxia cell culture device adopting the structure has better cell culture conditions, effectively simulates the intermittent hypoxia process, and controls the inflow of nitrogen and air through the switching control valve; the volume of the chamber is smaller, so that the using amount of nitrogen is saved, and the gas exchange control is more accurate; the real-time oxygen concentration detector carries out real-time monitoring on the oxygen concentration in the cavity by extracting trace gas in the cavity, the measured data is accurate, and the problem of gas pollution in the cavity is also avoided.
Publication (bulletin) No.: CN112226366A, publication (announcement) day: 2021-01-15 discloses a hypoxic cell culture device, including control center and culture chamber, the outer surface both sides of control center all are through No. two fixed blocks of screw fixed connection, the culture chamber top is provided with the bracing piece No. one, culture chamber upper surface both sides all bond a plurality of fixed block No. one, the inside one side of No. two fixed blocks closes soon and is connected with adjusting bolt, has all seted up a plurality of recess through a clear glass board and No. two clear glass inboard portions, is convenient for place the inside at a clear glass board and No. two clear glass boards with the culture dish of cell culture, all bonds simultaneously in the recess both sides of a clear glass board and No. two clear glass boards and has spacing spring group, and spacing through the spacing spring group culture dish of both sides to fine assurance culture dish is at the stability of placing of a clear glass board and No. two clear glass inboard portions.
Publication (bulletin) No.: CN206580840U, open (public) day: 2017-10-24 discloses a hypoxia environment carbon dioxide cell culture case, including outer door of case and oxygen supply bottle, one side of outer door of case is provided with the sealed pad of magnetism outer door, the opposite side of outer door of case is provided with the incubator main part, the surface of incubator main part is provided with control module, the lower extreme of incubator main part is provided with the supporting legs, the inside of incubator main part is provided with oxygen concentration control module, the inside of incubator main part is provided with electric heating layer, inner door liner and cultivates the chamber, the inside of cultivateing the chamber is provided with the shelf, the inside of electric heating layer is provided with electric heating wire, the oxygen supply bottle links to each other with the oxygen conveyer pipe. Through being provided with the sealed pad of magnetism outer door on the door limit at the outer door of case, can effectually prevent that the gas of outside from entering into and cultivateing the chamber, avoid causing the infection to the cell of the cultivation of inside, the outer door of case mainly adopts heating glass material to make, prevents to have the formation of condensate on the inner door.
Publication (bulletin) No.: CN204111768U, publication (announcement) date: 2015-01-21 provides a low-pressure hypoxic cell culture device, which comprises a hypoxic culture air chamber, a negative pressure aspirator, a pressure gauge and an oxygen concentration gauge, wherein the hypoxic culture air chamber comprises an air inlet and an air outlet, and valves are arranged on the air inlet and the air outlet; the air inlet is connected with the pressure gauge, the oxygen concentration gauge and the negative pressure aspirator in sequence through pipelines. Simple operation, can simulate the cell culture in the plateau low-pressure hypoxia environment, has no harm to people and effectively saves the research cost.
Technical scheme and the technical problem that will solve and the beneficial effect who produces of above disclosure technique all with the utility model discloses inequality, to the utility model discloses more technical characteristics and the technical problem and the beneficial effect that will solve all do not have the technological inspiration in the above technical document that discloses.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a portable cell hypoxia culture apparatus to the defect that prior art exists, solving the hypoxia incubator and being expensive, bulky be unfavorable for gaseous mixing, can expose in the cell shifts and the observation process shortcoming such as the air. Can provide the hypoxic culture environment for the cell, inputed to the culture box after the gas mixture for gas environment is more even stable. The culture box is small in size, transparent, convenient to observe and transfer, and low in price, and can be placed in a common carbon dioxide incubator.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a portable cell hypoxia culture device, which comprises a cell culture box;
still include gaseous mixing device, connect by the mist output tube between gaseous mixing device and the cell culture box, be equipped with the gas pump on the mist output tube.
Further, the gas blending device comprises a gas blending cabin;
the gas mixing cabin is provided with two gas inlets, a gas outlet and a mixed gas outlet;
the two air inlets are respectively connected with a carbon dioxide steel cylinder and a nitrogen steel cylinder through pipelines.
Further, the gas inlet and the gas outlet are both provided with a gas filter and a valve.
Further, the gas mixing cabin is equipped with 2 sensors, and 2 sensors are connected with outside carbon dioxide concentration detector and oxygen concentration detector respectively.
Furthermore, the cell culture box is provided with a drawer type door, a groove is formed in the contact position of the cell culture box and the drawer type door, the drawer type door is clamped into the groove, and a plastic sealing strip is arranged around the door.
Furthermore, the cell culture box is positioned above the water containing bottom groove, the bottom of the cell culture box is a porous bottom plate, and the distance from the bottom surface of the porous bottom plate to the bottom surface of the cell culture box is 3 cm.
Furthermore, the cell culture box is provided with a mixed gas inlet which is connected with a gas mixing device through a mixed gas output pipe.
Further, the cell culture box is provided with a cell box air outlet.
Compared with the prior art, the utility model following beneficial effect has:
(1) The utility model discloses the device divide into gaseous mixing device and cultivates box two parts, and gaseous after mixing device reaches appointed concentration and mixing, infuse to cultivateing in the box, has promoted gaseous homogeneity in the cultivation box.
(2) The utility model discloses the device can be connected a gaseous mixing device and a plurality of cell culture box through the multichannel pipeline, cultivates the independent each other before the box each other and does not influence, has reduced the influence of the in-process gas concentration change of taking to other cells.
(3) The cell culture box in the device is small in size, and can be placed in the same carbon dioxide incubator or constant temperature incubator, thereby reducing the cost and being economical and practical.
(4) The utility model discloses cell culture box in the device is transparent material, can direct observation to cell sap colour change and cell culture condition, need not take out cell bottle/ware, reduces the gas concentration change in the environment.
(5) The cell culture box in the device has flexible size and is convenient for transfer.
Drawings
FIG. 1 is a schematic structural view of a portable cell hypoxia culture device of the present invention.
In the figure: 1. the device comprises a gas mixing device, 2, a first mixing device gas inlet, 3, a second mixing device gas inlet, 4, a mixing device gas outlet, 5, a carbon dioxide concentration detector, 6, an oxygen concentration detector, 7, a mixed gas outlet, 8, a gas pump, 9, a cell culture box, 10, a drawer type door, 11, a porous bottom plate, 12, a water bottom groove, 13, a mixed gas inlet and 14, a cell box gas outlet.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1, the present invention provides a technical solution:
a portable cell hypoxia culture device comprises a gas mixing device 1 and a cell culture box 9 which are connected by a gas pipeline.
The gas mixing device 1 is provided with two gas inlets, namely a first mixing device gas inlet 2 and a second mixing device gas inlet 3 which are respectively connected with a nitrogen cylinder and a carbon dioxide cylinder, and different oxygen concentrations are realized by adjusting the gas inflow of nitrogen and carbon dioxide. The air inlets are all provided with air filters.
The gas blending device 1 is provided with a blending device gas outlet 4.
The gas blending device 1 is provided with 2 internal sensors which are respectively connected with a carbon dioxide detector 5 and an oxygen detector 6 to display the concentration of carbon dioxide and oxygen.
The gas mixing device 1 is provided with a mixed gas outlet 7 which is connected with a gas pipeline, and a gas filter is arranged in the pipeline. The pipeline is provided with a gas pump 8, and the mixed gas is input into the cell culture box through the pipeline.
The cell culture cassette 9 is a rectangular parallelepiped made of a transparent material, and has a size of 300mm × 200mm × 100mm (the size can be adjusted according to the cell culture dish or culture bottle in which it is placed).
The cell culture box 9 adopts a pull-type door 10, a groove is arranged at the contact position of the box body and the door, the pull-type door is clamped into the groove, and a plastic sealing strip is arranged around the door to ensure that the space is sealed and airtight.
Cell culture box 9 bottom is porous bottom plate 11, has 3 cm's distance apart from the bottom, can add sterile water in bottom dress water kerve 12, and in steam passed through porous entering culture box, the humidity of guaranteeing culture box.
The cell culture box 9 is provided with a mixed gas inlet 13 which is connected with the gas mixing device 1 through a pipeline. The mixed gas with certain oxygen concentration is infused into the culture box through a gas pump on the pipeline.
The cell culture box 9 is provided with an air outlet 14.
The cell culture box 9 can be placed in a common carbon dioxide incubator or a constant temperature incubator.
The gas mixing device comprises a gas mixing cabin, two gas inlets, a gas outlet and a mixed gas output pipe. The two air inlets are respectively connected with a carbon dioxide steel cylinder and a nitrogen steel cylinder through pipelines. And the gas inlet and the gas outlet are both provided with a gas filter and a valve. And a built-in sensor is arranged in the mixing cabin and is connected with an external carbon dioxide concentration detector and an external oxygen concentration detector. And the mixed gas output pipe is provided with a gas pump.
The cell culture box comprises a transparent cuboid box, a drawer-type opening door, a mixed gas inlet, a mixed gas outlet, a porous bottom plate and a bottom water tank. The culture box is made of transparent organic materials, and the size of the culture box is 300mm multiplied by 200mm multiplied by 100mm (the size can be adjusted according to the placed cell culture dish or culture bottle). The drawer type door opening is a drawing type door opening, and the bottom of the drawer type door opening is a porous bottom plate. The bottom water tank is 30mm away from the porous bottom plate in height and is used for injecting sterilizing distilled water to ensure the humidity in the box. The cell culture box can be placed in a normal carbon dioxide incubator and is connected with the outside through a mixed gas input pipeline and a mixed gas output pipeline. The mixed gas input port is connected with a gas mixing device through a pipeline, and a gas pump is arranged on the pipeline. And the mixed gas input and input pipelines are both provided with a gas filter and a valve.
When in use, the cell culture is carried out according to the following operation steps:
1) In the gas blending device 1, a gas inlet of a first blending device and a gas inlet of a second blending device are respectively connected with a carbon dioxide gas cylinder and a nitrogen gas cylinder, the gas inflow is adjusted, a gas outlet 4 of the blending device is opened, and a mixed gas outlet 7 is closed until a carbon dioxide concentration detector 5 and an oxygen concentration detector 6 display the concentration of target gas.
2) The cell culture box 9 is opened, and sterilized distilled water is added into the water bottom groove 12 to increase the humidity of the culture box.
3) The petri dish or flask is placed on the perforated floor 11 by means of the drawer-type door 10.
4) And opening the mixed gas output port 7, the mixed gas input port 13 and the gas pump 8, infusing the uniformly mixed gas with the target concentration into the cell culture box 9, opening the gas outlet 14 of the cell culture box, and maintaining the pressure balance in the box.
5) The cell culture cassette 9 was placed in a normal carbon dioxide incubator and maintained at a culture temperature of 37 ℃.
All parts and parts which are not discussed in the present application and the connection mode of all parts and parts in the present application belong to the known technology in the technical field, and are not described again.
In the present application, the term "plurality" means two or more unless expressly defined otherwise. The terms "mounted," "connected," "fixed," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a removable connection, or an integral connection; "coupled" may be direct or indirect through an intermediary. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the description of the present invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "back", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or unit indicated must have a specific direction, be constructed and operated in a specific orientation, and therefore, should not be construed as limiting the present invention.
In the description of the present specification, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A portable cell hypoxia culture device comprises a cell culture box;
the cell culture box is characterized by further comprising a gas mixing device, wherein the gas mixing device is connected with the cell culture box through a mixed gas output pipe, and a gas pump is arranged on the mixed gas output pipe.
2. The portable cellular hypoxia culture device according to claim 1, wherein the gas blending device comprises a gas blending chamber;
the gas mixing chamber is provided with two gas inlets, a gas outlet and a mixed gas outlet;
the two air inlets are respectively connected with a carbon dioxide steel cylinder and a nitrogen steel cylinder through pipelines.
3. A portable hypoxia culture device according to claim 2, wherein the gas inlet and outlet are fitted with gas filters and valves.
4. The portable cell hypoxia culture device according to claim 2 or 3, wherein the gas mixing chamber is provided with 2 sensors, and the 2 sensors are respectively connected with an external carbon dioxide concentration detector and an external oxygen concentration detector.
5. The portable hypoxia culture device for cells according to claim 4, wherein the cell culture box is provided with a drawer-type door, a groove is arranged at the contact position of the cell culture box and the drawer-type door, the drawer-type door is clamped into the groove, and a plastic sealing strip is arranged around the door.
6. The portable cellular hypoxia culture device according to claim 5, wherein the cell culture box is arranged above a water-containing bottom tank, the bottom of the cell culture box is a porous bottom plate, and the bottom surface of the porous bottom plate is 3cm away from the bottom surface of the cell culture box.
7. A portable cellular hypoxia culture device according to claim 5, wherein the cell culture box is provided with a mixed gas inlet, and is connected with the gas mixing device through a mixed gas outlet pipe.
8. A portable cellular hypoxia culture device according to claim 7, wherein the cell culture box is provided with a cell box air outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221989298.XU CN218058989U (en) | 2022-07-30 | 2022-07-30 | Portable cell hypoxia culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221989298.XU CN218058989U (en) | 2022-07-30 | 2022-07-30 | Portable cell hypoxia culture device |
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| Publication Number | Publication Date |
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| CN218058989U true CN218058989U (en) | 2022-12-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202221989298.XU Active CN218058989U (en) | 2022-07-30 | 2022-07-30 | Portable cell hypoxia culture device |
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| CN (1) | CN218058989U (en) |
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- 2022-07-30 CN CN202221989298.XU patent/CN218058989U/en active Active
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