CN210711573U - Group B streptococcus culture apparatus - Google Patents
Group B streptococcus culture apparatus Download PDFInfo
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- CN210711573U CN210711573U CN201920991765.4U CN201920991765U CN210711573U CN 210711573 U CN210711573 U CN 210711573U CN 201920991765 U CN201920991765 U CN 201920991765U CN 210711573 U CN210711573 U CN 210711573U
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Abstract
The utility model discloses a B crowd's streptococcus culture apparatus, including device body and micro-fluidic chip, be equipped with the constant head tank that is used for placing micro-fluidic chip on the device body, the constant head tank bottom is equipped with the control by temperature change board, micro-fluidic chip includes the bottom plate that sets gradually from bottom to top, middle level and upper strata, the through-hole that runs through that is equipped with on the middle level forms with the bottom plate and cultivates the pond, is equipped with B crowd's streptococcus culture medium in the cultivation pond, upper strata inside is equipped with and cultivates the inlet channel of pond intercommunication, inlet channel and introduction port intercommunication, be equipped with buckle and draw-in groove on the device body, buckle one end is articulated with the device body, and the cabin is cultivateed to the inside formation of draw-in groove to the other end card income, be. The utility model discloses can realize the cultivation and the appraisal of B crowd's streptococcus, sample consumption is few, and check-out time is short, and sensitivity is high, can avoid the mutual interference of cultivateing the thing in the pond simultaneously, and the device is small and exquisite, convenient to use.
Description
Technical Field
The utility model relates to a microbial cultivation technical field especially relates to a B crowd's streptococcus culture apparatus.
Background
Streptococcus agalactiae (group B) normally inhabits the vagina and the intestinal tract of a human body, the bacteria carrying rate can reach about 30%, the bacteria can cause early fulminant septicemia and late-onset pyogenic meningitis of newborns, and the fatality rate is about 15%. Adult group B streptococcal infections include bacteremia, endocarditis, skin and soft tissue infections, and osteomyelitis. Bacterial infection can cause various diseases, and the disease rate is high, and critical illness state is often caused, so that timely diagnosis and treatment are needed.
The traditional bacteria identification method is to spread a body fluid specimen of a patient on an agar plate containing a culture medium for culturing and enriching bacteria, then select dominant bacteria for culturing and identifying and carry out drug sensitivity experiments. The method has the problems of large sample consumption and long detection time, and often cannot effectively meet the requirements of clinical work. In addition, the traditional bacteria identification method mostly depends on a plurality of large specialized devices, and the popularization of the technology in the primary medical unit is limited. Therefore, a simple, rapid and portable bacteria identification platform should be developed to meet the urgent need of clinical work.
The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like.
The microfluidic chip has the characteristic of flexible structural design, is convenient for realizing integrated and high-throughput analysis under the condition of small size, and is very suitable for the culture and identification of high-throughput bacteria due to the advantage. Therefore, the invention provides a novel group B streptococcus culture device based on the microfluidic technology.
Disclosure of Invention
The utility model aims to provide a: the utility model provides a B crowd's streptococcus culture apparatus, can realize the cultivation and the appraisal to B crowd's streptococcus, the sample consumption is few, and the check-out time is short, and sensitivity is high, can avoid the mutual interference of material in the culture pond simultaneously, and the device is small and exquisite, convenient to use.
The utility model adopts the technical scheme as follows:
a group B streptococcus culture device comprises a device body and a microfluidic chip, wherein a positioning groove for placing the microfluidic chip is arranged on the device body, a temperature control plate is arranged at the bottom of the positioning groove, the microfluidic chip comprises a bottom plate, a middle layer and an upper layer which are sequentially arranged from bottom to top, a through hole formed in the middle layer and the bottom plate form a culture pond, a group B streptococcus culture medium is arranged in the culture pond, a sample introduction channel communicated with the culture pond is arranged in the upper layer and is communicated with a sample introduction port, compared with the traditional culture detection device, the use of the microfluidic chip greatly reduces the sample amount, improves the test flux, shortens the sensitivity and the test time, a buckle plate and a clamp groove are arranged on the device body, one end of the buckle plate is hinged with the device body, the other end of the buckle plate can be clamped into the clamp groove to form a culture cabin, and a, the pressing block is pressed between the adjacent culture ponds when the pinch plate is closed, and the microfluidic chip and the temperature control plate are both arranged in the culture cabin.
The utility model discloses a theory of operation does:
when the micro-injection device is used, group B streptococcus suspension is slowly injected into a sample inlet on the micro-fluidic chip through the micro-injector, the bacterial suspension flows through the first sample inlet channel and the second sample inlet channel and is sequentially filled with all culture pools, the micro-fluidic chip is placed in the positioning groove after sample injection is completed, the chip is placed on the temperature control plate, the buckle plate is clamped and fixed with the clamping groove on the device body, the pressing block on the buckle plate is just pressed between the adjacent culture pools at the moment, the adjacent culture pools are separated, mutual interference among the culture pools is limited by the micro-valve effect, the temperature control plate provides proper temperature for growth of group B streptococcus, the group B streptococcus culture medium in the culture pool provides nutrients for bacterial growth, and monitoring of the group B streptococcus can be realized by monitoring color change in the culture pool by a user. In addition, various culture media can be added into the culture pond according to actual needs, and the culture and detection of various bacteria can be realized simultaneously.
Further, the temperature control plate comprises a base plate, a heater and a heating aluminum plate which are sequentially arranged from bottom to top, and a temperature sensor is arranged inside the heating aluminum plate. The heater and the heating aluminum plate play a role in heating the microfluidic chip, and the temperature sensor can monitor the temperature constantly, so that a constant temperature environment is provided for the growth of bacteria.
Furthermore, the sampling channel comprises a first sampling channel and a second sampling channel, the first sampling channel and the second sampling channel are sequentially communicated, the first sampling channel is communicated with the sampling port, and the second sampling channel is communicated with the culture pond. The bacteria flow into the second sample feeding channel through the first sample feeding channel, and the second sample feeding channel is communicated with the culture pools, so that the corresponding culture pools are filled with the bacteria, and after the culture pools are filled with the bacteria, the bacteria continuously flow and are sequentially filled into the next culture pool until the culture pools are completely filled.
Furthermore, the second sample feeding channel is concentric with the culture pond, and has the same radius, so that bacteria can flow into the culture pond quickly and timely.
Furthermore, the bottom plate, the middle layer and the upper layer of the microfluidic chip are all made of PDMS materials. The PDMS material has certain air permeability and is suitable for culturing aerobic bacteria such as group B streptococcus.
Furthermore, a water storage tank is arranged at the bottom of the temperature control plate. A proper amount of sterilizing water containing bacteriostatic agents is injected into the water storage tank, and the culture cabin is ensured to have enough humidity under the heating condition so as to inhibit the evaporation of water in the chip.
Furthermore, a control panel and a display screen are arranged on the device body. The control panel and the display screen are convenient for operators to use and control, and the operation is simple and convenient.
Furthermore, the pinch plate is made of transparent materials. The operator can directly observe the growth condition of bacteria through the pinch plate.
To sum up, owing to adopted above-mentioned technical scheme, the beneficial effects of the utility model are that:
1. the utility model discloses a B crowd's streptococcus culture apparatus is a microanalysis platform, can reduce the sample consumption effectively and improve the test flux for traditional large-scale professional equipment, and sensitivity has obtained the improvement.
2. The utility model discloses a culture and the appraisal of bacterium can be realized in step to B crowd's streptococcus culture apparatus, has saved the enrichment step for traditional method, therefore has showing and has shortened the analysis time.
3. The utility model discloses a culture apparatus is easy and simple to handle, and is small and exquisite portable, is suitable for the on-the-spot short-term test of reply.
4. The utility model discloses when the buckle was closed, the briquetting on the buckle just in time was pressed between adjacent cultivation pond, separates adjacent cultivation pond, plays the micro-valve effect and can restrict the mutual interference between cultivation pond.
5. The utility model discloses a device is not restricted to the cultivation and the detection of B crowd's streptococcus, can also add multiple culture medium as required in the culture pond, realizes the cultivation and the detection of multiple bacterium simultaneously.
Drawings
The invention will now be described, by way of example, with reference to the accompanying drawings, in which:
fig. 1 is a first schematic structural diagram of the present invention;
FIG. 2 is a second schematic structural diagram of the present invention;
FIG. 3 is a schematic view of a thermal control plate;
FIG. 4 is a schematic structural diagram of a microfluidic chip;
FIG. 5 is a view showing the structure of the mounting of the thermal control plate and the microfluidic chip;
FIG. 6 is a view showing the structure of the mounting of the thermal control plate and the microfluidic chip when the pinch plate is closed.
Labeled as: 1-device body, 11-card slot, 2-control panel, 3-display screen, 4-buckle, 41-briquetting, 5-positioning slot, 6-microfluidic chip, 61-upper layer, 611-sample inlet, 612-sample channel, 613-first sample channel, 614-second sample channel, 62-middle layer, 621-culture tank, 63-bottom plate, 7-temperature control plate, 71-substrate, 72-heater, 73-heating aluminum plate, 74-temperature sensor, 8-water storage tank.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
A group B streptococcus culture device comprises a device body 1 and a microfluidic chip 6, wherein a positioning groove 5 for placing the microfluidic chip 6 is arranged on the device body 1, a temperature control plate 7 is arranged at the bottom of the positioning groove 5, the microfluidic chip 6 comprises a bottom plate 63, a middle layer 62 and an upper layer 61 which are sequentially arranged from bottom to top, a through hole formed in the middle layer 62 and the bottom plate 63 form a culture pool 621, a group B streptococcus culture medium is arranged in the culture pool 621, a sample introduction channel 612 communicated with the culture pool 621 is arranged in the upper layer 61, the sample introduction channel 612 is communicated with a sample introduction port 611, compared with a traditional culture detection device, the use of the microfluidic chip 6 greatly reduces the sample amount, improves the test flux, shortens the sensitivity and the test time, a buckle plate 4 and a clamping groove 11 are arranged on the device body 1, one end of the buckle plate 4 is hinged with the device body 1, the other end of the clamping plate can be clamped into the clamping groove 11 to form a culture cabin, a pressing block 41 is arranged on the clamping plate 4, the pressing block 41 is pressed between the adjacent culture ponds 621 when the clamping plate 4 is closed, and the microfluidic chip 6 and the temperature control plate 7 are arranged in the culture cabin.
The utility model discloses a theory of operation does:
when the micro-injection device is used, group B streptococcus suspension is slowly injected into the sample inlet 611 on the micro-fluidic chip 6 through the micro-injector, the bacterial suspension flows through the first sample injection channel 613 and the second sample injection channel 614 and is sequentially filled in all the culture pools 621, the micro-fluidic chip 6 is placed in the positioning groove 5 after sample injection is completed, the chip is placed on the temperature control plate 7, the buckle plate 4 is clamped and fixed with the clamping groove 11 on the device body 1, the pressing block 41 on the buckle plate 4 is just pressed between the adjacent culture pools 621, the adjacent culture pools 621 are separated, the micro-valve effect is achieved, mutual interference among the culture pools 621 is limited, the temperature control plate 7 provides proper temperature for growth of group B streptococcus, the group B streptococcus culture medium in the culture pools 621 provides nutrients for bacterial growth, and monitoring of group B streptococcus can be achieved by monitoring color change in the culture pools 621 by a user. In addition, various culture media can be added into the culture pond 621 according to actual needs, and the culture and detection of various bacteria can be realized simultaneously.
Example 2
On the basis of embodiment 1, the temperature control plate 7 includes a base plate 71, a heater 72 and a heating aluminum plate 73, which are sequentially arranged from bottom to top, and a temperature sensor 74 is arranged inside the heating aluminum plate 73. The heater 72 and the heating aluminum plate 73 heat the microfluidic chip 6, and the temperature sensor 74 can monitor the temperature at any time, so as to provide a constant temperature environment for the growth of bacteria.
Example 3
On the basis of embodiment 1, the sample channel 612 comprises a first sample channel 613 and a second sample channel 614, the first sample channel 613 and the second sample channel 614 are sequentially communicated, the first sample channel 613 is communicated with the sample inlet 611, and the second sample channel 614 is communicated with the culture cell 621. The bacteria flow into the second sample feeding channel 614 through the first sample feeding channel 613, and since the second sample feeding channel 614 is communicated with the culture cells 621, the bacteria are filled in the corresponding culture cells 621, and after the culture cells 621 are filled, the bacteria continue to flow and sequentially fill the next culture cell 621 until the culture cells 621 are completely filled.
Example 4
On the basis of embodiment 3, the second sample inlet channel 614 is concentric with the culture pond 621, and has the same radius, so as to facilitate bacteria to flow into the culture pond 621 quickly in time.
Example 5
On the basis of the embodiment 1, the bottom plate 63, the middle layer 62 and the upper layer 61 of the microfluidic chip 6 are made of PDMS materials. The PDMS material has certain air permeability and is suitable for culturing aerobic bacteria such as group B streptococcus.
Example 6
On the basis of any one of embodiments 1-5, a water storage tank 8 is arranged at the bottom of the temperature control plate 7. A proper amount of sterilizing water containing bacteriostatic agents is injected into the water storage tank 8, and the culture cabin is ensured to have enough humidity under the heating condition so as to inhibit the evaporation of water in the chip.
Example 7
On the basis of the embodiment 1, the device body 1 is provided with a control panel 2 and a display screen 3. The control panel 2 and the display screen 3 are convenient for operators to use and control, and the operation is simple and convenient.
Example 8
On the basis of the embodiment 1, the pinch plate 4 is made of a transparent material. The operator can observe the growth of bacteria directly through the pinch plate 4.
Claims (8)
1. The utility model provides a B crowd's streptococcus culture apparatus, includes device body (1) and micro-fluidic chip (6), a serial communication port, be equipped with constant head tank (5) that are used for placing micro-fluidic chip (6) on device body (1), constant head tank (5) bottom is equipped with temperature control plate (7), micro-fluidic chip (6) are including bottom plate (63) that set gradually from bottom to top, middle level (62) and upper strata (61), the through-hole that runs through that is equipped with on middle level (62) forms with bottom plate (63) and cultivates pond (621), is equipped with B crowd's streptococcus culture medium in cultivateing pond (621), upper strata (61) inside be equipped with and cultivate sampling channel (612) of pond (621) intercommunication, sampling channel (612) and introduction port (611) intercommunication, be equipped with buckle (4) and draw-in groove (11) on device body (1), buckle (4) one end is articulated with device body (1), the other end can be blocked into the clamping groove (11) to form a culture cabin, a pressing block (41) is arranged on the buckling plate (4), and the pressing block (41) is pressed between the adjacent culture ponds (621) when the buckling plate (4) is closed.
2. The group B streptococcus culture apparatus according to claim 1, wherein the temperature control plate (7) comprises a base plate (71), a heater (72) and a heating aluminum plate (73) which are arranged from bottom to top, and a temperature sensor (74) is arranged inside the heating aluminum plate (73).
3. A group B streptococcus culture apparatus according to claim 1, wherein the sample introduction channel (612) comprises a first sample introduction channel (613) and a second sample introduction channel (614), the first sample introduction channel (613) and the second sample introduction channel (614) are communicated in sequence, the first sample introduction channel (613) is communicated with the sample introduction port (611), and the second sample introduction channel (614) is communicated with the culture cell (621).
4. A group B streptococcus culture apparatus according to claim 3, wherein said second sample inlet channel (614) is concentric with the culture bath (621) and has the same radius.
5. The apparatus for culturing group B streptococcus according to claim 1, wherein the base plate (63), the middle layer (62) and the upper layer (61) of the microfluidic chip (6) are made of PDMS material.
6. The group B streptococcus culture apparatus according to any one of claims 1-5, wherein a water storage tank (8) is arranged at the bottom of the temperature control plate (7).
7. A group B streptococcus culture apparatus according to claim 1, wherein said apparatus body (1) is provided with a control panel (2) and a display screen (3).
8. The group B streptococcus culture apparatus according to claim 1, wherein said gusset plate (4) is made of transparent material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920991765.4U CN210711573U (en) | 2019-06-27 | 2019-06-27 | Group B streptococcus culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920991765.4U CN210711573U (en) | 2019-06-27 | 2019-06-27 | Group B streptococcus culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210711573U true CN210711573U (en) | 2020-06-09 |
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ID=70964145
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920991765.4U Expired - Fee Related CN210711573U (en) | 2019-06-27 | 2019-06-27 | Group B streptococcus culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210711573U (en) |
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2019
- 2019-06-27 CN CN201920991765.4U patent/CN210711573U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200609 Termination date: 20210627 |