CN214881492U - Fermentation inoculation device - Google Patents
Fermentation inoculation device Download PDFInfo
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- CN214881492U CN214881492U CN202121041033.2U CN202121041033U CN214881492U CN 214881492 U CN214881492 U CN 214881492U CN 202121041033 U CN202121041033 U CN 202121041033U CN 214881492 U CN214881492 U CN 214881492U
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Abstract
The utility model provides a fermentation inoculation device, including the inoculation bottle, the fermentation cylinder, the inoculation bottle has the intermediate layer outward, water inlet and delivery port have on the intermediate layer, be connected with the exit tube in the export of inoculation bottle, install valve A on the exit tube, install first quick-operation joint on the exit tube between the export of valve A and inoculation bottle, first quick-operation joint comprises first connection A and first connection B, be connected with second quick-operation joint on the pipeline behind valve A, second quick-operation joint is connected A by the second and is connected B with the second and constitute, A is connected through being connected between pipeline and the valve A to the second, B is connected through being connected between pipeline and the valve B to the second, valve B connects on advancing the pipe of fermentation cylinder, be connected with valve C on the side pipe of valve A's end. The fermentation inoculation device can avoid fermentation failure due to bacteria contamination.
Description
Technical Field
The utility model relates to a biological field's fermentation inoculation device, in particular to inoculation device of aseptic technique when being applicable to the fermentation inoculation belongs to bio-pharmaceuticals equipment technical field.
Background
In the fermentation production, the inoculation of a fermentation tank is a crucial link, and the sterility degree of the inoculation directly determines the success or failure of the fermentation. The current inoculation mode is to put the culture medium into a fermentation tank, after high-temperature sterilization, when the culture medium is cooled to the temperature required by the process, the cultured strains are transferred into the fermentation tank through an inoculation bottle, and the transfer process generally adopts a flame inoculation method or a differential pressure inoculation method. The flame inoculation method is to form a small-range sterile environment around the flame, but the inoculation bottle is not in the protection range of the flame, the inoculation ports on the inoculation bottle and the fermentation tank are opened for inoculation during inoculation, and the inoculation ports are exposed in the air and are easy to contaminate bacteria; the differential pressure inoculation method is to fix the inoculation bottle on the fermentation tank, open the valve on fermentation tank and the inoculation bottle, utilize the pressure differential with the fungus seed solution impress in the fermentation tank, the shortcoming lies in the in-process of realizing inoculation bottle and fermentation tank, has local exposure, although follow-up through fermentation tank sterilization, still can leave the dead angle, can't realize aseptic technique, leads to the fermentation tank to contaminate the fungus, the fermentation failure.
Disclosure of Invention
The utility model aims to overcome the problems existing in the prior fermentation inoculation and provide a fermentation inoculation device.
In order to realize the purpose of the utility model, the following technical proposal is adopted: the utility model provides a fermentation inoculation device, including inoculation bottle, fermentation cylinder, the inoculation bottle have the intermediate layer outward, water inlet and delivery port have on the intermediate layer, be connected with the exit tube in the export of inoculation bottle, install valve A on the exit tube, install first quick-operation joint on the exit tube between the export of valve A and inoculation bottle, first quick-operation joint comprises first connection A and first connection B, be connected with the second quick-operation joint on the pipeline behind valve A, the second quick-operation joint is connected A by the second and is connected B with the second and constitute, the second is connected A and is connected between through pipeline and valve A, the second is connected B and is connected between through pipeline and valve B, valve B connects on advancing the pipe of fermentation cylinder, be connected with valve C on valve A's the other pipe of terminal, when valve A closes, be the intercommunication state between A and the valve C is connected to the second.
Further, the method comprises the following steps of; the water inlet on the interlayer is positioned at the outlet end of the inoculation bottle, and the water outlet is positioned at the bottom end of the inoculation bottle.
Further, the method comprises the following steps of; the inoculation bottle is provided with a temperature detector.
Further, the method comprises the following steps of; and silica gel pads are arranged in the first quick joint and the second quick joint.
The utility model discloses an actively beneficial technological effect lies in: the fermentation inoculation device can realize all-dimensional dead-angle-free sterilization in inoculation, realize aseptic operation and avoid fermentation failure due to bacterial contamination.
Drawings
Fig. 1 is an overall schematic view of the present invention.
Detailed Description
In order to explain the utility model more fully, the utility model provides an implementation example. These examples are merely illustrative of the present invention and do not limit the scope of the present invention.
The invention is explained in further detail with reference to the drawings, in which the reference symbols are: 1: inoculating bottles; 2: an interlayer; 3: a water inlet; 4: a water outlet; 5: a temperature detector; 6: a first connection A; 7: a first connection B; 8: a valve A; 9: a valve C; 10: a second connection A; 11: a second connection B; 12: a valve B; 13: and (4) fermenting the mixture.
As shown in the attached drawing, the fermentation inoculation device comprises an inoculation bottle 1 and a fermentation tank 13, wherein an interlayer 2 is arranged outside the inoculation bottle, a water inlet 3 and a water outlet 4 are arranged on the interlayer, the water inlet on the interlayer is positioned at the outlet end of the inoculation bottle, and the water outlet is positioned at the bottom end of the inoculation bottle. The inoculation bottle is provided with a temperature detector 5.
The outlet of the inoculation bottle is connected with an outlet pipe, the outlet pipe is provided with a valve A8, the outlet pipe between the valve A and the outlet of the inoculation bottle is provided with a first quick joint, the first quick joint is composed of a first connection A6 and a first connection B7, a second quick joint is connected on a pipeline behind the valve A, the second quick joint is composed of a second connection A10 and a second connection B11, the second connection A10 is connected with the valve A8 through a pipeline, the second connection B11 is connected with the valve B12 through a pipeline, the valve B is connected on an inlet pipe of the fermentation tank, a valve C12 is connected on a side pipe at the tail end of the valve A, when the valve A is closed, the second connection A and the valve C are in a communication state, namely, after the valve C is a closing surface of the valve A, the communication state between the second connection A and the valve C is not influenced after the valve A is closed. And silica gel pads are arranged in the first quick joint and the second quick joint. Two quick-operation joint all adopt stainless steel, and quick-operation joint accessible market is bought and is obtained, and the silica gel pad also can obtain simultaneously when buying quick-operation joint.
In the device, the second connection A, the valve A and the first quick connector are connected on the inoculation bottle.
The use process of the fermentation inoculation device is as follows:
1. before inoculation of the fermentation tank, the inoculation bottle is placed into a sterilizer for high-temperature sterilization, after cooling, the valve A is closed, the inoculation bottle is moved into a sterile room for aseptic operation, the first quick-assembly connection is opened, cultured strain liquid is moved into the inoculation bottle, the first quick-assembly connection is connected, the inoculation bottle is moved out of the sterile room and is ready for inoculation with the fermentation tank; in the process, only the rear end of the closing surface of the valve A is possibly contaminated by bacteria;
2. fixing a second quick connection A on the inoculation bottle and a second quick connection B on the fermentation tank, introducing circulating water into an upper interlayer of the inoculation bottle for butt joint, opening a valve C and a valve B, sterilizing the fermentation tank, sterilizing all pipelines at the rear end of a closed surface of the valve A by steam discharged from the fermentation tank when the fermentation tank is sterilized, cooling the inoculation bottle by the circulating water in the interlayer during sterilization, and preventing high temperature from being conducted to the inoculation bottle; the fermentation tank sterilization process is the prior art in fermentation inoculation;
3. and after the sterilization is finished, closing the valve C and the valve B, cooling the fermentation tank, and cooling the fermentation tank to a temperature within the process control range.
Starting inoculation, opening a valve A and a valve B, increasing the pressure of the fermentation tank to 0.1mpa, and then closing the valve B to enable the pressure in an inoculation bottle to be about 0.1 mpa;
5. reducing the pressure in the fermentation tank to about 0.05mpa, opening the valve B, pressing the strain liquid in the inoculation bottle into the fermentation tank by utilizing the pressure difference, closing the valve B, opening the valve C, exhausting the pressure in the inoculation bottle, and finishing the inoculation.
The above-described differential pressure inoculation is currently the state of the art.
After the embodiments of the present invention have been described in detail, those skilled in the art can clearly understand that various changes and modifications can be made without departing from the scope and spirit of the above claims, and any simple modification, equivalent change and modification made to the above embodiments according to the technical spirit of the present invention all fall within the scope of the technical solution of the present invention, and the present invention is not limited to the embodiments of the examples given in the specification.
Claims (4)
1. The utility model provides a fermentation inoculation device, includes inoculation bottle, fermentation cylinder, its characterized in that: the inoculation bottle outside have the intermediate layer, water inlet and delivery port have on the intermediate layer, be connected with the exit tube in the export of inoculation bottle, install valve A on the exit tube, install first quick-operation joint on the exit tube between the export of valve A and inoculation bottle, first quick-operation joint comprises first connection A and first connection B, be connected with the second quick-operation joint on the pipeline behind valve A, the second quick-operation joint is connected A by the second and is connected B and constitute, the second is connected A and is connected between pipeline and the valve A, the second is connected B and is connected between through pipeline and valve B, valve B connects on advancing of fermentation cylinder manages, be connected with valve C on the other pipe of valve A's end, when valve A closes, be the connected state between second connection A and the valve C.
2. A fermentation inoculation device according to claim 1, wherein: the water inlet on the interlayer is positioned at the outlet end of the inoculation bottle, and the water outlet is positioned at the bottom end of the inoculation bottle.
3. A fermentation inoculation device according to claim 1, wherein: the inoculation bottle is provided with a temperature detector.
4. A fermentation inoculation device according to claim 1, wherein: and silica gel pads are arranged in the first quick joint and the second quick joint.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121041033.2U CN214881492U (en) | 2021-05-17 | 2021-05-17 | Fermentation inoculation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121041033.2U CN214881492U (en) | 2021-05-17 | 2021-05-17 | Fermentation inoculation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214881492U true CN214881492U (en) | 2021-11-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121041033.2U Active CN214881492U (en) | 2021-05-17 | 2021-05-17 | Fermentation inoculation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214881492U (en) |
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2021
- 2021-05-17 CN CN202121041033.2U patent/CN214881492U/en active Active
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