CN106244434A - A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation and making and application - Google Patents

Abstract

The invention discloses an inoculation device suitable for microbial fertilizer liquid fermentation and its production and application, comprising a bottle body, a bottle mouth, a bottle cap and a silicone tube. It is equipped with an ultraviolet lamp, and the space around the ultraviolet lamp is filled with high-temperature-resistant transparent glue. The bottle mouth is welded on the bottle body. The glass two, bottle cap and bottle mouth are potted and sealed with high temperature resistant transparent glue two, and an LED ultraviolet lamp is installed between the glass and the bottle cap; the bottle mouth is welded in the center of the bottle cap, and a silicone tube is set on the upper end of the bottle mouth, and the silicone tube is additionally One end is provided with a quick-connect valve; the metal connection parts are all welded by argon arc welding. The design of the device is scientific and reasonable, simple to operate, low in cost, easy to clean, can effectively prevent bacterial infection, has good sealing properties, and has wide applicability to the field of liquid fermentation inoculation.

Description

An inoculation device suitable for microbial fertilizer liquid fermentation and its production and application

technical field

The invention mainly relates to the field of microbial fertilizers, in particular, the invention relates to the field of liquid fermentation of microbial fertilizers.

Background technique

my country has a long history of biological fermentation technology, and the fermentation process mainly includes two types: solid state and liquid state. Compared with the solid state, the liquid state fermentation method has been widely researched and applied because of its advantages such as short fermentation cycle, high equipment utilization rate, and high fermentation yield. As far as liquid fermentations such as vinegar and soy sauce are concerned, most of the current reports are the surface static fermentation method and the liquid submerged fermentation method. The surface static fermentation method is a traditional method. Although it is simple and easy to implement, the production cycle is long, the equipment occupies a large area, the utilization rate of raw materials is low, and the product quality is unstable; High standard.

As a green and environmentally friendly fertilizer, microbial fertilizer has been praised and used by all walks of life for a long time. The effect of microbial fertilizer on increasing production and income is remarkable. Microbial fertilizer provides beneficial microorganisms that can fix nitrogen, decompose phosphorus, and decompose potassium. These living microorganisms can grow in the rhizosphere of plants. Growth and reproduction can bring several benefits: First, through the life activities of these beneficial microorganisms, the molecular nitrogen that cannot be used in the air can be fixed and transformed into compound nitrogen, and the compound phosphorus and potassium that cannot be used in the soil can be analyzed into available nitrogen. Utilize the state of phosphorus and potassium, and can analyze more than 10 kinds of medium and trace elements in the soil. The second is to secrete auxin, cytokinin, gibberellin, indolic acid and other plant hormones through the life activities of these beneficial microorganisms to promote crop growth, regulate crop metabolism, and build high-quality products according to genetic codes. The third is through the massive reproduction of beneficial microorganisms in the rhizosphere to produce a large amount of mucopolysaccharides, which combine with the mucus secreted by plants, mineral colloids, and organic colloids to form a soil aggregate structure and improve the soil's ability to store fertilizer and water. Good-quality microbial fertilizers can promote crop growth, improve soil structure, improve crop product quality, and improve crop disease prevention and disease resistance, thereby increasing production and income.

However, the use of liquid submerged fermentation technology to produce microbial fertilizers needs to ensure that the inoculation process is safe and pollution-free. At present, the commonly used inoculation technology is to press the bacteria in the inoculation bottle into the seed tank. The disadvantage is that the upper and lower silica gel plugs of the suction filter bottle and the suction filter bottle itself cannot be combined into an effective unity. When the bottle is pressurized, the bacteria in the inoculation bottle will run, run, drip, and leak, and the risk of bacterial contamination is high. The glass material has poor pressure bearing capacity, and there is a danger of bursting the bottle, which poses a safety hazard. After being made into a stainless steel bottle, the internal ultraviolet sterilization cannot be achieved because the material is opaque.

Contents of the invention

Aiming at the defects of the existing technology, that is, the upper and lower silica gel plugs of the suction filter bottle and the suction filter bottle itself cannot be combined into an effective unity during the cultivation of strains, and when the pressure is applied to the bottle, the strains in the inoculation bottle will run, burst, Dripping and leaking, the risk of bacterial contamination is high, and the glass material has poor pressure bearing capacity, there is a risk of bursting bottles, and there are potential safety hazards. The present invention aims to provide an inoculation device suitable for microbial fertilizer liquid fermentation and its production and application. The principle is to solve the problem of easy self-contamination, difficult to clean and poor pressure bearing capacity of the inoculation bottle by using argon arc welding technology, 304 stainless steel material and unique size ratio to solve the problems of the inoculation bottle. And triangular thread design enhances the sealing effect.

The present invention specifically provides an inoculation device suitable for microbial fertilizer liquid fermentation, including a bottle body, a bottle mouth, a bottle cap and a silicone tube. Welded into a triangular conical shape, the bottom of the bottle is equipped with tempered glass, which is sandwiched with the bottom of the bottle body, and the interlayer is equipped with a UV lamp. The gap around the UV lamp is filled with high-temperature resistant transparent glue. The height is 393mm-465mm; the bottle mouth is welded on the upper end of the bottle body, and there is an external thread outside the bottle mouth. The cap is provided with an internal thread, and the internal thread provided on the inside of the bottle cap is adapted to the external thread provided on the outside of the bottle mouth. The tempered glass two, bottle cap and bottle mouth are potted with high-temperature resistant transparent glue two, and an LED ultraviolet lamp is installed between the glass and the bottle cap; a 10mm hole is opened in the center of the bottle cap, and the mouth of the bottle with a length of 40mm-50mm is welded. The mouth is made of 304 stainless steel steel pipe with an outer diameter of 10 mm; a silicone tube is provided at the upper end of the bottle mouth, and a quick-connect valve is provided at the other end of the silicone tube; the metal connection parts are all welded by argon arc welding.

In the present invention, the quick-connect valve at the end of the silicone tube is provided with a high-temperature-resistant rubber sleeve.

In the present invention, the stainless steel thickness of the bottle body and the bottle cap is 2mm-3mm.

In the present invention, the diameter of the bottom of the bottle is 208mm, and the height of the bottle wall is 393mm.

In the present invention, the outer diameter of the bottle mouth is 45mm, and the bottle mouth height is 50mm.

In the present invention, the outside of each quick-connect valve is equipped with a stainless steel hoop.

In the present invention, the bottom of the bottle adopts a flat-bottomed stainless steel head that is pressed or spun by a flat plate.

In the present invention, the bottle wall is pressed and welded by a conical plate rolling machine.

Simultaneously, the present invention provides a kind of preparation method that is applicable to the inoculation device of microbial fertilizer liquid fermentation, specifically comprises following steps making:

(1) Making the bottom of the bottle: Make the bottom of the bottle with a flat-bottomed or spinning stainless steel flat-bottomed head, glue the UV lamp in the bottom of the bottle, punch the bottle cap to lead the line out of the bottom of the bottle, and use high-temperature-resistant transparent glue Install tempered glass under UV lamp after potting.

(2) Bottle wall production: Use a conical plate rolling machine to press and weld the bottle body.

(3) Mouth production: use cylindrical ordinary external thread, the thread is a triangular tooth shape, multi-thread helical thread, and make a bottle mouth with external thread.

(4) Weld the processed bottle body, bottle mouth and bottle bottom with argon arc welding to form a triangular interlayer stainless steel triangular bottle with an outer threaded bottle mouth.

(5) Bottle cap production: use the internal thread cap as the bottle cap, triangular tooth shape, multi-wire helical wire, glue the LED ultraviolet lamp in the bottle cap, punch the bottle cap and lead the thread out of the bottle cap, and place it on the LED Install the tempered glass II under the ultraviolet lamp, and seal it with high-temperature-resistant transparent glue II.

Further, the present invention provides an application of an inoculation device suitable for microbial fertilizer liquid fermentation in the microbial fertilizer liquid fermentation process, specifically comprising the following steps:

(1) Rinse with tap water until there is no dirt, then wash with detergent or soak in 0.5% detergent aqueous solution, carefully brush the inside and outside of the inoculation bottle, rinse with tap water, and put it upside down in a clean place to dry for later use.

(2) Wrap the mouth and cap of the bottle with medical gauze, wrap it with two layers of newspaper or kraft paper, and tie it tightly with a thin cotton string, put a suitable high-temperature-resistant rubber plug on the other end of the silicone tube, and wrap it with kraft paper and tie it tightly with a cotton string ;Sterilize the wrapped inoculation bottle based on damp heat at 121.3°C for 45min-60min, and then transfer to the sterile room to open the room and sterilize the bottle with ultraviolet light for 20min-30min for standby.

(3) Move the cultivated shake flask seeds into the aseptic closed bottle, remove the mouth of the inoculation bottle and the protective gauze and protective paper at the mouth of the shake flask under the protection of alcohol flame or aseptic operation technology, and put the cultivated Shake the seeds of the bottle into the stainless steel inoculation bottle, and finally fit the body of the inoculation bottle with the cap, and wait for the seed tank to be inoculated.

(4) Under the protection of the alcohol flame, remove the high-temperature-resistant rubber sleeve on the quick-connect valve of the silicone tube of the inoculation bottle, quickly insert the quick-connect valve into the inoculation port, and adjust the pressure of the seed tank to below 0.08Mpa; fully open the inoculation port Valve, pour the seed solution into the tank in stages with differential pressure; close the inoculation valve, remove the quick-connect valve of the inoculation bottle; screw on the protective cover, and close the stopcock; adjust the tank pressure, temperature and air volume to start fermentation.

Implementation of the inoculation device provided by the invention for the liquid fermentation of microbial fertilizers obtains the following beneficial effects:

The invention provides an inoculation device suitable for microbial fertilizer liquid fermentation and its application, which is scientific and reasonable in design, simple in operation, low in cost, easy to clean, can effectively prevent miscellaneous bacteria infection, has good sealing characteristics, and has great advantages in the field of liquid fermentation inoculation. Broad applicability.

Description of drawings

Fig. 1 shows a schematic diagram of the structure of the inoculation device provided by the present invention.

Fig. 2 shows a schematic diagram of the cross-sectional structure of the bottle in the inoculation device provided by the present invention.

Fig. 3 shows a schematic diagram of the cross-sectional structure of the bottle cap in the inoculation device provided by the present invention.

In Figure 1-3, 1-bottle body, 11-ultraviolet lamp, 12-high temperature resistant transparent glue 1, 13-tempered glass 1, 2-bottle mouth, 21-external thread, 3-bottle cap, 31-bottle mouth , 32-LED ultraviolet lamp, 33-high temperature resistant transparent glue 2, 34-tempered glass 2, 35-internal thread, 4-silicone tube, 41-quick valve.

detailed description

The specific implementation of the present invention will be further described in detail below with reference to the accompanying drawings 1-3 and the embodiments, but the device of the present invention is not limited to the following embodiments.

In the present invention, for ease of description, in an inoculation device suitable for microbial fertilizer liquid fermentation in liquid fermentation of microbial fertilizer, the description of the relative positional relationship of each component is based on the layout of accompanying drawings 1-3 For the description, for example, positional relationships such as up, down, left, and right are determined according to the layout direction of FIG. 1 .

Embodiment one:

The present invention provides an inoculation device suitable for microbial fertilizer liquid fermentation, including a bottle body (1), a bottle mouth (2), a bottle cap (3) and a silicone tube (4), and the bottle body (1) is made of 304 stainless steel , including two parts, the bottle wall and the bottle bottom, the bottle bottom and the bottle wall are welded into a triangular conical shape, the bottom of the bottle is provided with a tempered glass (13) and the bottom of the bottle body (1) in a sandwich shape, and an ultraviolet lamp (11) is arranged in the interlayer ), the space around the ultraviolet lamp (11) is potted with high-temperature-resistant transparent glue (12), the diameter of the bottom of the bottle is 208 mm, and the height of the bottle wall is 393 mm; the bottle mouth (2) is welded on the upper end of the bottle body (1), and the bottle mouth (2) An external thread (21) is provided on the outside, and the external thread (21) adopts a triangular tooth shape and is provided with a multi-thread helix. The outer diameter of the bottle mouth (2) is 45mm, and the height of the bottle mouth (2) is 50mm; Internal thread (35), the internal thread (35) provided on the inner side of the bottle cap (3) is adapted to the external thread (21) provided on the outer side of the bottle mouth (2), the thread line is triangular tooth shape, and multi-thread thread line is provided , the inner top of the bottle cap (3) is provided with tempered glass two (34), and the space between the tempered glass two (34), the bottle cap (3) and the bottle mouth (31) is potted with high-temperature-resistant transparent glue two (33), An LED ultraviolet lamp (31) is arranged between the tempered glass 2 (34) and the bottle cap (3); a 10mm hole is opened in the center of the bottle cap (3), and a bottle mouth (31) with a length of 40mm is welded, and the bottle mouth (31) adopts 304 stainless steel steel pipe with an outer diameter of 10 mm; a silicone tube (4) is provided at the upper end of the bottle mouth (31), and a quick-connect valve (41) is provided at the other end of the silicone tube (4); the metal connection parts are all welded by argon arc welding.

In the present invention, the quick-connect valve (41) at the end of the silicone tube (4) is provided with a high-temperature-resistant rubber sleeve.

In the present invention, the stainless steel thickness of the bottle body (1) and the bottle cap (3) is 2mm.

Embodiment two:

The present invention provides an inoculation device suitable for microbial fertilizer liquid fermentation, including a bottle body (1), a bottle mouth (2), a bottle cap (3) and a silicone tube (4), and the bottle body (1) is made of 304 stainless steel , including two parts, the bottle wall and the bottle bottom, the bottle bottom and the bottle wall are welded into a triangular conical shape, the bottom of the bottle is provided with a tempered glass (13) and the bottom of the bottle body (1) in a sandwich shape, and an ultraviolet lamp (11) is arranged in the interlayer ), the space around the ultraviolet lamp (11) is potted with high-temperature-resistant transparent glue (12), the diameter of the bottom of the bottle is 290 mm, and the height of the bottle wall is 465 mm; the bottle mouth (2) is welded on the upper end of the bottle body (1), and the bottle mouth (2) An external thread (21) is provided on the outside, and the external thread (21) adopts a triangular tooth shape and is provided with a multi-thread helix. The outer diameter of the bottle mouth (2) is 63.5mm, and the height of the bottle mouth (2) is 60mm; There is an internal thread (35), and the internal thread (35) provided on the inside of the bottle cap (3) is adapted to the external thread (21) provided on the outside of the bottle mouth (2). line, the inner top of the bottle cap (3) is provided with tempered glass two (34), and the tempered glass two (34) and the bottle cap (3) and bottle mouth (31) are filled and sealed by high temperature resistant transparent glue two (33) , an LED ultraviolet lamp (31) is arranged between the tempered glass 2 (34) and the bottle cap (3); a 10 mm hole is opened in the center of the bottle cap (3), and a bottle mouth (31) with a length of 50 mm is welded to the bottle mouth (31) 304 stainless steel steel pipe with an outer diameter of 10mm is used; the upper end of the bottle mouth (31) is provided with a silicone tube (4), and the other end of the silicone tube (4) is provided with a quick-connect valve (41); the metal connection parts are all welded by argon arc welding .

In the present invention, the quick-connect valve (41) at the end of the silicone tube (4) is provided with a high-temperature-resistant rubber sleeve.

In the present invention, the stainless steel thickness of the bottle body (1) and the bottle cap (3) is 3mm.

Embodiment three:

The present invention provides an inoculation device suitable for microbial fertilizer liquid fermentation, including a bottle body (1), a bottle mouth (2), a bottle cap (3) and a silicone tube (4), and the bottle body (1) is made of 304 stainless steel , including two parts, the bottle wall and the bottle bottom, the bottle bottom and the bottle wall are welded into a triangular conical shape, the bottom of the bottle is provided with a tempered glass (13) and the bottom of the bottle body (1) in a sandwich shape, and an ultraviolet lamp (11) is arranged in the interlayer ), the space around the ultraviolet lamp (11) is filled with high-temperature-resistant transparent glue (12), the diameter of the bottom of the bottle is 260 mm, and the height of the bottle wall is 430 mm; the bottle mouth (2) is welded on the upper end of the bottle body (1), and the bottle mouth (2) The outer thread (21) is provided with an external thread (21), which adopts a triangular tooth shape and is provided with a multi-thread helix. The outer diameter of the bottle mouth (2) is 53.5mm, and the height of the bottle mouth (2) is 55mm; There is an internal thread (35), and the internal thread (35) provided on the inside of the bottle cap (3) is adapted to the external thread (21) provided on the outside of the bottle mouth (2). Toughened glass 2 (34) is arranged on the inner top of the bottle cap (3), and the space between the tempered glass 2 (34) and the bottle cap (3) and bottle mouth (31) is potted with high-temperature-resistant transparent glue 2 (33) , an LED ultraviolet lamp (31) is arranged between the tempered glass 2 (34) and the bottle cap (3); a 10 mm hole is opened in the center of the bottle cap (3), and a bottle mouth (31) with a length of 45 mm is welded to the bottle mouth (31) 304 stainless steel steel pipe with an outer diameter of 10mm is used; the upper end of the bottle mouth (31) is provided with a silicone tube (4), and the other end of the silicone tube (4) is provided with a quick-connect valve (41); the metal connection parts are all welded by argon arc welding .

In the present invention, the stainless steel thickness of the bottle body (1) and the bottle cap (3) is 2.5 mm.

Embodiment four:

Raw materials: the high temperature resistant transparent glue used in the present invention is produced by Wacker Company in Germany

The present invention further provides a method for making an inoculation device suitable for microbial fertilizer liquid fermentation as follows:

Making the bottom of the bottle: make the bottom of the bottle with flat-bottomed or spinning stainless steel flat-bottomed head, glue the ultraviolet lamp (11) in the bottom of the bottle, punch the bottle cap (3) and lead the thread out of the bottom of the bottle, durable Install toughened glass one (13) under the ultraviolet lamp (11) after potting with high-temperature transparent glue one (12).

Bottle wall production: Use a conical plate rolling machine to press and weld the bottle body (1).

Mouth production: use cylindrical common external thread, the thread is triangular tooth shape, multi-thread helical thread, and make the bottle mouth with external thread (2).

The processed bottle body (1), bottle mouth (2) and bottle bottom are welded by argon arc welding to form a triangular interlayer stainless steel triangular bottle with an outer threaded bottle mouth.

Bottle cap production: select the internal thread cap as the bottle cap (3), triangular tooth shape, multi-thread helix, glue the LED ultraviolet lamp (31) in the bottle cap (3), and punch the bottle cap (3) Lead the wire out of the bottle cap (3), install tempered glass two (34) under the LED ultraviolet lamp (31), and use high-temperature-resistant transparent glue two (33) for potting.

Embodiment five:

The application of the inoculation device suitable for microbial fertilizer liquid fermentation in the process of microbial fertilizer liquid fermentation, the specific application process includes the following steps:

(1) Rinse with tap water until there is no dirt, then wash with detergent or soak in 0.5% detergent aqueous solution, carefully brush the inside and outside of the inoculation bottle, rinse with tap water, and put it upside down in a clean place to dry for later use.

(2) Wrap the bottle mouth (2) and bottle cap (3) with medical gauze, wrap it with two layers of newspaper or kraft paper, and tie it tightly with a thin cotton string. Put on a suitable high-temperature-resistant rubber stopper, wrap it with kraft paper and tie it tightly with a cotton string; sterilize the wrapped inoculation bottle based on damp heat at 121.3°C for 45min-60min, and then transfer to the sterile room to open the room and sterilize the inside of the bottle with a UV lamp for 20min -30 min standby.

(3) Transfer the cultivated shaker flask seeds into the aseptic interlocking bottle, and remove the mouth of the inoculation bottle (2) and the protective gauze and protective paper of the shaker bottle mouth under the protection of alcohol flame or aseptic operation technology, and put The cultured shake flask seeds are poured into the stainless steel inoculation bottle, and finally the inoculation bottle body (1) and the bottle cap (3) are combined, waiting for the seed tank to be inoculated.

(4) Under the protection of the alcohol flame, remove the high-temperature-resistant rubber sleeve on the quick-connect valve (41) of the inoculation bottle, quickly insert the quick-connect valve (41) into the inoculation port, and adjust the pressure of the seed tank to below 0.08Mpa; Fully open the valve of the inoculation port, and pour the seed solution into the tank in stages using the differential pressure; close the inoculation valve, remove the quick-connect valve (41) of the inoculation bottle; screw on the protective sleeve, and close the stopcock; adjust the tank pressure, temperature and air volume Start the fermentation run.

As mentioned above, the present invention can be better realized. The above-mentioned embodiment is only a description of the preferred implementation of the present invention, and does not limit the scope of the present invention. Various modifications and improvements made by technicians to the technical solution of the present invention shall fall within the scope of protection determined by the present invention.

Claims (10)

1. being applicable to a classification inoculation apparatus for microbial manure liquid fermentation, including bottle, bottleneck, bottle cap and silica gel tube, it is special Levying and be, bottle uses 304 stainless steels, including two parts at the bottom of bottle wall and bottle, at the bottom of bottle and bottle wall is welded into triangular pyramidal, bottle It is sandwich-like that the end is provided with safety glass one with bottle body bottom, is provided with uviol lamp, the resistance to height in space around uviol lamp in interlayer Temperature transparent glue one embedding, diameter 208mm-290mm at the bottom of bottle, bottle wall height 393mm-465mm；Bottleneck is welded on bottle upper end, Bottleneck is outside equipped with external screw thread, and external screw thread uses triangular tooth-shaped, is provided with multi-thread helix, bottleneck external diameter 45mm-63.5mm, bottle Open height 50mm-60mm；Bottle cap is provided with female thread, the external screw thread phase of setting outside female thread and the bottleneck arranged inside bottle cap Adapting to arrange, threaded line becomes triangular tooth-shaped, is provided with multi start thread line, and in bottle cap, top ends is provided with safety glass two, tempering By thermostable transparent glue two embedding between glass two and bottle cap, bottle neck, between glass and bottle cap, it is provided with LED uviol lamp； Opening 10mm hole, the bottle neck of weld length 40mm-50mm in bottle cap central authorities, bottle neck uses 304 stainless-steel pipes of external diameter 10mm；Bottle Mouth upper end is provided with silica gel tube, and silica gel tube other end is provided with quick-joint valve；Described metallic connection parts position all uses argon arc welding Welding.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that silica gel The quick-joint valve of pipe end is outside equipped with resistant to elevated temperatures rubber sleeve.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that bottle It is 2mm-3mm with the rustless steel thickness of bottle cap.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that at the bottom of bottle Diameter 208mm, bottle wall height 393mm.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that bottleneck External diameter 45mm, bottleneck height 50mm.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that each Stainless steel lathe dog it is equipped with outside quick-joint valve.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that at the bottom of bottle Use flat board compacting or the rustless steel flat bottom cover of spinning.

A kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation, it is characterised in that bottle wall Use the compacting welding of taper veneer reeling machine.

9. a kind of classification inoculation apparatus of microbial manure liquid fermentation that is applicable to as described in any one of claim 1-8 is in microorganism Application in fertilizer liquid sweat, it is characterised in that application process comprises the following steps:

(1) rinse extremely without dirt with tap water, then wash with detergent or be soaked in 0.5% detergent aqueous solution, by inoculation bottle Inside and outside carefully scrub after, rinse well with tap water, be upside down at cleaning dry standby；

(2) bottleneck and bottle cap hospital gauze are encased, double-bag outward newspaper or kraft paper, and tighten with thin lace cord, at silicon Sebific duct other end suitable high-temperature resistant rubber plug beyond the Great Wall, outsourcing kraft paper lace cord tightens, by the inoculation bottle that bandages based on 121.3 DEG C of moist heat sterilization 45min-60min, then proceed to aseptic unlatching room and internal uviol lamp sterilizing 20 min-of bottle 30min is standby；

(3) cultured shake-flask seed is moved into aseptic and closes bottle, tear open under Alcohol Flame is protected or under aseptic behaviour's technical protection Drive inoculation bottle bottleneck and shaking flask bottleneck protection gauze and protection sheet, pour cultured shake-flask seed into rustless steel inoculation bottle, After inoculation bottle bottle and bottle cap is fit, wait seed tank inoculation；

(4) under the protection of Alcohol Flame, the high-temperature resistant rubber set on inoculation bottle silica gel tube quick-joint valve is taken off, rapidly by quick-joint valve It is inserted on inoculation mouth, seed tank pressure is adjusted to below 0.08Mpa, standard-sized sheet inoculation mouth valve, by several times kind of liquid is poured into pressure reduction In tank, close inoculation valve, take off inoculation bottle quick-joint valve, protection set of screwing on, fasten plug valve, modulation tank pressure, temperature and air quantity are opened Originate ferment to run.

10. the making of a kind of classification inoculation apparatus being applicable to microbial manure liquid fermentation as described in any one of claim 1-8 Method, specifically includes following steps and makes:

(1) make at the bottom of bottle and make: use the rustless steel flat bottom cover of flat board compacting or spinning to make at the bottom of bottle, bonding at the bottom of bottle Uviol lamp, outside bottle cap punching being drawn at the bottom of bottle by line, with installing tempering glass after thermostable transparent glue one embedding under uviol lamp Glass one；

(2) bottle wall makes: use taper veneer reeling machine compacting welding bottle；

(3) bottleneck makes: using the common external screw thread of cylinder, screw thread is triangular tooth-shaped, multi-thread helix, is fabricated to carry externally threaded Bottleneck；

(4) will process and weld with argon arc welding at the bottom of bottle, bottleneck, bottle, and form one and carry outer stricture of vagina silk bottleneck triangular interlayer stainless Steel triangular flask；

(5) bottle cap makes: selection female thread pipe cap is as bottle cap, triangular tooth-shaped, multi-thread helix, bonding LED in bottle cap Uviol lamp, draws outside bottle cap by bottle cap punching by line, installs safety glass two, and use thermostable transparent glue under LED uviol lamp Water two embedding.