CN201542986U - Sterilization apparatus for inoculation port of pilot fermentation tank - Google Patents

Sterilization apparatus for inoculation port of pilot fermentation tank Download PDF

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Publication number
CN201542986U
CN201542986U CN 200920253138 CN200920253138U CN201542986U CN 201542986 U CN201542986 U CN 201542986U CN 200920253138 CN200920253138 CN 200920253138 CN 200920253138 U CN200920253138 U CN 200920253138U CN 201542986 U CN201542986 U CN 201542986U
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CN
China
Prior art keywords
inoculation
valve
pipeline
subcultivation
pilot scale
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200920253138
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Chinese (zh)
Inventor
刘培政
张�杰
赵保翠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LUZHOU FOOD PRODUCT GROUP CORP Ltd SHANDONG PROV
Original Assignee
LUZHOU FOOD PRODUCT GROUP CORP Ltd SHANDONG PROV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LUZHOU FOOD PRODUCT GROUP CORP Ltd SHANDONG PROV filed Critical LUZHOU FOOD PRODUCT GROUP CORP Ltd SHANDONG PROV
Priority to CN 200920253138 priority Critical patent/CN201542986U/en
Application granted granted Critical
Publication of CN201542986U publication Critical patent/CN201542986U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination

Abstract

The utility model discloses a sterilization apparatus for an inoculation port of a pilot fermentation tank, which belongs to a sterilization device of a fermentation system and solves the problem that in the prior art, the inoculation pipeline is insufficiently sterilized and is easy to cause microbiological contamination of the inoculating process. The sterilization apparatus mainly comprises a three-way pipe, an inoculation pipeline, a sterilizing chamber and a valve. The inoculation pipeline is provided with the inoculation port. One end of the sterilizing chamber is connected with the inoculation pipeline by a clamping buckle and can be freely separated. The sterilizing chamber is provided with a pressure gauge. The sterilization apparatus for the inoculation port of the pilot fermentation tank is mainly used in the field of the microorganism pilot fermentation and can also be used for other industries requiring to be sterilized.

Description

Pilot scale fermentation jar inoculation mouthful bactericidal unit
Technical field
This utility model relates to a kind of pilot scale fermentation jar inoculation mouthful bactericidal unit, belongs to the fermentation system sterilizing installation.
Background technology
In the microorganism pilot scale fermentation process, need carry out the steam sterilization of subcultivation pipeline and valve before the main fermentation tank subcultivation from seed tank, reach a subcultivation pipeline completely without the bacterium condition, this is the precondition of an indispensability of sweat.The pilot scale fermentation equipment of present conventional design, the subcultivation pipeline adopts thin and long stainless steel pipes to be made, and there is not a pressure and temperature display device, it is not in place to be easy to form the pipeline sterilization, cause the microbiological contamination of subcultivation process, thereby directly cause the failure of fermentation, form enormous economic loss.
Summary of the invention
Not in place in order to overcome in the prior art sterilization of subcultivation pipeline, cause the defective of subcultivation process microbiological contamination easily, this utility model provides an a kind of pilot scale fermentation jar inoculation mouthful bactericidal unit.
This utility model is realized by the following technical solutions: a kind of pilot scale fermentation jar inoculation mouthful bactericidal unit, comprise a tee T, be respectively equipped with valve 2, valve 3, valve 4 on three ports of tee T, the other end of valve 4 is provided with the subcultivation pipeline, and the subcultivation pipeline is provided with the inoculation mouth; A described pilot scale fermentation jar inoculation mouthful bactericidal unit is provided with the sterile chamber, and an end of sterile chamber links to each other with the subcultivation pipeline by buckle and can freely separate, and the other end of sterile chamber is provided with valve 5, the sterile chamber is provided with Pressure gauge.
Can be provided with sealing gasket between described buckle and the subcultivation pipeline; Described sealing gasket is the tetrafluoro mat preferably; Can be provided with the ring-type non-slip groove on the described inoculation mouth; Described Pressure gauge is the rustless steel diaphragm preferably.
Compared with prior art, the beneficial effects of the utility model are: use this utility model from seed tank to the main fermentation tank subcultivation, can exempt carefully growing and cause steam sterilization microbiological contamination phenomenon not in place, thereby reach the purpose of normal fermentation because of the subcultivation pipeline; Can avoid the fermentation failure effectively and cause the tremendous economic loss to enterprise.
Description of drawings
Fig. 1 is this utility model structural representation;
Fig. 2 is a user mode reference diagram of the present utility model.
The specific embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
In the accompanying drawing: 1, seed tank; 2, valve; 3, valve; 4, valve; 5, valve; 6, buckle; 7, sterile chamber; 8, inoculation mouth; 9, Pressure gauge; 10 tee Ts; 11, subcultivation pipeline
Embodiment:
A kind of pilot scale fermentation jar inoculation mouthful bactericidal unit comprises a tee T 10 and sterile chamber 7; Be respectively equipped with valve 2, valve 3, valve 4 on three ports of tee T 10, the other end of valve 4 is provided with subcultivation pipeline 11, and subcultivation pipeline 11 is provided with inoculation mouth 8; One end of sterile chamber 7 links to each other with subcultivation pipeline 11 by buckle 6 and can freely separate, and the other end of sterile chamber 7 is provided with valve 5, sterile chamber 7 is provided with rustless steel diaphragm 9; Be provided with the tetrafluoro mat between buckle 6 and the subcultivation pipeline 11; Inoculation mouth 8 is provided with the ring-type non-slip groove.
When the seed of seed tank reaches before subcultivation requires soon, at first this utility model is connected with the jar mouth of seed tank 1 by valve 2.In addition, needing to prepare a silica gel tube also sterilizes standby as the subcultivation flexible pipe in advance.
Valve 2 is closed all the time during sterilization; Open valve 4 and 5 at first, Open valve 3 slowly again allows steam entering slowly, adjust valve 5, allow the pressure in the sterile chamber 7 remain on 0.12Mpa, and kept 30-40 minute, sterilization is closed valve 3 and 5 rapidly after finishing simultaneously, allows keep malleations in the sterile chamber 7; The seed of seed tank 1 arrives when requiring; under the protection of Alcohol Flame, slowly open buckle 6; take off sterile chamber 7; the subcultivation flexible pipe of bacterium of will having gone out also is inserted in inoculation mouthfuls 8 rapidly under the protection of Alcohol Flame; and prick and go up band (the steriliser operation of fermentation tank near-end is with the steriliser of seed tank near-end), open valve 2, utilize pressure differential that the seed in the seed tank 1 is pressed into fermentation tank; after finishing, inoculation closes the valve of fermentation tank near-end, the fermentation beginning.

Claims (5)

1. a pilot scale fermentation jar inoculation mouthful bactericidal unit comprises a tee T, is respectively equipped with valve (2), valve (3), valve (4) on three ports of tee T, and the other end of valve (4) is provided with the subcultivation pipeline, and the subcultivation pipeline is provided with the inoculation mouth; It is characterized in that: a described pilot scale fermentation jar inoculation mouthful bactericidal unit is provided with the sterile chamber, and an end of sterile chamber links to each other with the subcultivation pipeline by buckle and can freely separate, and the other end of sterile chamber is provided with valve (5), and the sterile chamber is provided with Pressure gauge.
2. pilot scale fermentation jar inoculation mouthful bactericidal unit according to claim 1 is characterized in that: be provided with sealing gasket between described buckle and the subcultivation pipeline.
3. pilot scale fermentation jar inoculation mouthful bactericidal unit according to claim 2, it is characterized in that: described sealing gasket is the tetrafluoro mat.
4. pilot scale fermentation jar inoculation mouthful bactericidal unit as claimed in claim 1 is characterized in that: described inoculation mouth is provided with the ring-type non-slip groove.
5. pilot scale fermentation jar inoculation mouthful bactericidal unit as claimed in claim 1, it is characterized in that: described Pressure gauge is the rustless steel diaphragm.
CN 200920253138 2009-11-12 2009-11-12 Sterilization apparatus for inoculation port of pilot fermentation tank Expired - Fee Related CN201542986U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200920253138 CN201542986U (en) 2009-11-12 2009-11-12 Sterilization apparatus for inoculation port of pilot fermentation tank

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200920253138 CN201542986U (en) 2009-11-12 2009-11-12 Sterilization apparatus for inoculation port of pilot fermentation tank

Publications (1)

Publication Number Publication Date
CN201542986U true CN201542986U (en) 2010-08-11

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200920253138 Expired - Fee Related CN201542986U (en) 2009-11-12 2009-11-12 Sterilization apparatus for inoculation port of pilot fermentation tank

Country Status (1)

Country Link
CN (1) CN201542986U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103571750A (en) * 2013-07-10 2014-02-12 温州兆曜机械科技有限公司 Method for improving inoculation process through aseptic operation box

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103571750A (en) * 2013-07-10 2014-02-12 温州兆曜机械科技有限公司 Method for improving inoculation process through aseptic operation box
CN103571750B (en) * 2013-07-10 2015-06-17 温州兆曜机械科技有限公司 Method for improving inoculation process through aseptic operation box

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
EE01 Entry into force of recordation of patent licensing contract

Assignee: Lu Zhou Biotechnology (Shaanxi) Co., Ltd.

Assignor: Luzhou Food Product Group Corp., Ltd., Shandong Prov.

Contract record no.: 2012610000022

Denomination of utility model: Sterilization apparatus for inoculation port of pilot fermentation tank

Granted publication date: 20100811

License type: Exclusive License

Record date: 20120327

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20100811

Termination date: 20141112

EXPY Termination of patent right or utility model