CN106834422B - Full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule - Google Patents
Full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule Download PDFInfo
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- CN106834422B CN106834422B CN201710029497.3A CN201710029497A CN106834422B CN 106834422 B CN106834422 B CN 106834422B CN 201710029497 A CN201710029497 A CN 201710029497A CN 106834422 B CN106834422 B CN 106834422B
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Abstract
The invention discloses a kind of full-automatic assembly line collection bacterium operating method and system towards the bottled aqueous solution test sample of ampoule, including the first manipulator, ampoule bottle box, first hose installs auxiliary body, second manipulator, first peristaltic pump, surge tank/profit film liquid/cleaning solution/medium bottle box, second peristaltic pump, hose current limliting/heat sealing mechanism, third manipulator, collect bacterium bottle rack, yellow cap stopper, red cap stopper, 4th manipulator, the scrap concretes such as hose area, 5th manipulator, second hose installs auxiliary body, 6th manipulator, collect bacterium bottle/hose/cap stopper box, 7th manipulator, 8th manipulator, ampoule bottle opener, the recovery areas such as reagent bottle.The present invention uses multipaths string, parallel optimization method to the bottled aqueous solution test sample of ampoule to be checked, realizes that continuous, efficient microorganism is enriched with automatically, greatly improves operating efficiency, and reduce erroneous detection risk.
Description
Technical field
The invention belongs to sterility test system automation technical fields, are related to one kind towards the bottled aqueous solution test sample of ampoule
Full-automatic assembly line collection bacterium operating method and system.
Technical background
According to version " Chinese Pharmacopoeia " in 2015, the Sterility Test of test sample included membrane-filter procedure and direct inoculation.
Membrane-filter procedure should generally use closed membrane filter.The filter sizes of sterility test should be not more than 0.45 μm, diameter
About 50mm.Test sample includes: aqueous solution test sample, water-soluble solid test sample, water-insoluble test sample, dissolves in the tetradecane
Descendants' agent of isopropyl propionate and stickiness finish test sample, sterile gas (spray) mist agent test sample, the syringe test sample equipped with drug, tool
There is medical apparatus (blood transfusion, infusion bag etc.) test sample etc. of conduit.Meanwhile the amount of inspection and inspection of " Chinese Pharmacopoeia " to test sample
The amount of testing also is made that specific regulation.As it can be seen that be faced with during Sterility testing Multi-example type, more specification pieces packaging,
The problem of multipaths process analysis procedure analysis, noncontinuity operation, low collection bacterium analysis efficiency.
During existing Sterility testing, it is faced with Multi-example type, more specification pieces packaging, multipaths process point
The problem of analysis, noncontinuity operation, low collection bacterium analysis efficiency, it is common to use manual mode completes relevant operation, automation, intelligence
Change degree is lower；The factors that are influenced by personnel of critical process such as the operation of collection bacterium, cleaning are larger, also can due to different personnel operate band
Carry out the significant difference of sterility test result and efficiency.
Summary of the invention
During Sterility testing manual mode complete relevant operation there are aiming at the problem that, the present invention propose towards ampoule bottle
Fill aqueous solution test sample full-automatic assembly line collection bacterium operating method and system, it is characterised in that using string, parallel processing and
Flow optimization method, comprising the following steps:
Step (1) clamps ampoule bottle box, surge tank/profit film liquid/cleaning solution/medium bottle box, collection bacterium bottle/soft on guide rail
Pipe/cap stopper box is respectively put into fixed position.
Step (2) opens collection bacterium bottle/hose/cap stopper box, and the hose with syringe needle being connected with collection bacterium bottle is installed into second
Peristaltic pump, while the another set of hose with syringe needle in box being installed into the first peristaltic pump.
Step (3) installs the hose with syringe needle being connected with collection bacterium bottle into hose current limliting/heat sealing mechanism；Bacterium will be collected simultaneously
Bottle is put into collection bacterium bottle rack, collects the yellow cap stopper in bacterium bottle/hose/cap stopper box, red cap stopper is put into fixed position.
The syringe needle being mounted on the hose in the second peristaltic pump is inserted into surge tank/profit film liquid/cleaning solution/training by step (4)
It supports on the profit film liquid bottle in base bottle box, opens the second peristaltic pump after being inverted profit film liquid bottle, profit film liquid is pumped into collection bacterium bottle to micro-
Hole filter membrane is soaked, and closes the second peristaltic pump after profit film liquid reaches certain capacity.
Red cap stopper lid is entered to collect bacterium top of bottle by step (5), opens the second peristaltic pump after just setting profit film liquid bottle, gas is pumped into
It is pressurized in collection bacterium bottle, so that profit film liquid closes the second peristaltic pump after profit film liquid is completely exhausted out completely from collection bacterium bottle discharge,
Red cap stopper is removed to fixed position.
Ampoule bottle in ampoule bottle box is sent to ampoule bottle and opened by step (6) while step (2) to step (5) carries out
It opens in mechanism and is opened, sent back in ampoule bottle box after unlatching.
Syringe needle on the hose one end being mounted in the first peristaltic pump is inserted by step (7) while step (6) carry out
Buffering in the ampoule bottle having turned on, in syringe needle insertion surge tank/profit film liquid/cleaning solution/medium bottle box of the hose other end
On tank, opens the first peristaltic pump and the aqueous solution test sample in ampoule bottle is pumped into surge tank；It is supplied to the aqueous solution in ampoule bottle
After test product is pumped into surge tank completely, the first peristaltic pump is closed；Syringe needle is removed into the peace that the ampoule bottle finished has turned on to other
In small jar bottle, opens the first peristaltic pump and the aqueous solution test sample in ampoule bottle is pumped into surge tank.
Step (8) will moisten film liquid bottle after the aqueous solution test sample in the ampoule bottle of all feedings is pumped into surge tank completely
On syringe needle extract and be inserted on surge tank, be inverted surge tank simultaneously open the second peristaltic pump, by aqueous solution test sample be pumped into collection bacterium
In bottle, the second peristaltic pump is closed after aqueous solution test sample is transferred to completely in collection bacterium bottle.
Red cap stopper lid is entered to collect bacterium top of bottle by step (9), opens the second peristaltic pump after just setting surge tank, and gas is pumped into collection
It is pressurized in bacterium bottle, aqueous solution test sample is filtered by the miillpore filter in collection bacterium bottle and microorganism is enriched with, works as aqueous solution
Test sample closes the second peristaltic pump after collection bacterium bottle discharge completely, removes red cap stopper to fixed position.
Syringe needle on surge tank is extracted and is inserted on cleaning solution bottle by step (10), is inverted cleaning solution bottle and unlatching second is compacted
Cleaning solution is pumped into collection bacterium bottle, the second peristaltic pump is closed after cleaning solution reaches certain capacity by dynamic pump.
Red cap stopper lid is entered to collect bacterium top of bottle by step (11), the second peristaltic pump is opened after just setting cleaning solution bottle, by gas pump
Enter to collect and be pressurized in bacterium bottle, the cleaning to miillpore filter is realized in cleaning solution discharge from collection bacterium bottle, when cleaning solution is completely exhausted out
After close the second peristaltic pump, remove red cap stopper to fixed position.
Yellow cap stopper is moved to bottom and the indentation of collection bacterium bottle by step (12), is sealed, is prevented water clock.
Syringe needle on cleaning solution bottle is extracted and is inserted on the medium bottle equipped with a certain culture medium by step (13), is closed simultaneously
It closes with after second, third hose for being connected of collection bacterium bottle, is inverted medium bottle and simultaneously opens the second peristaltic pump, culture medium is pumped into the
In one collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with same medium by step (14), together
When close with first, after the hose that third collection bacterium bottle is connected, be inverted medium bottle simultaneously the second peristaltic pump of unlatching, culture medium pumped
Enter in second collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with different culture medium by step (15), together
When close with after the first, second hose for being connected of collection bacterium bottle, be inverted medium bottle simultaneously the second peristaltic pump of unlatching, culture medium pumped
Enter in third collection bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity.
Red cap stopper lid is entered to collect bacterium top of bottle by step (16), and it is soft to cut three for simultaneously sealing and being connected with collection bacterium bottle simultaneously
Pipe.
Step (17) surge tank/profit film liquid/cleaning solution/medium bottle puts back to surge tank/profit film liquid/cleaning solution/culture medium
In bottle box, while the hose with syringe needle on the first peristaltic pump and the second peristaltic pump being removed, all wastes is transferred to soft
Guan Deng scrap concrete area.
Step (18) resets all devices to original state, waits the automatic collection bacterium behaviour of next batch aqueous solution test sample
Make.
Furtherly, the opening time of whole ampoule bottles in ampoule bottle box and whole aqueous solution test samples are transferred to buffering
The sum of time of tank should be less than being equal to peace of the hose being connected with collection bacterium bottle in the second peristaltic pump, hose current limliting/heat sealing mechanism
The sum of the wetting of miillpore filter, efflux time in ETL estimated time of loading and collection bacterium bottle.
Furtherly, aqueous solution test sample type to be checked need to meet the requirements the flushing dose of cleaning solution, if cleaning
Amount is more than single bottle cleaning solution, needs to increase by two steps after step 11:
Syringe needle on first cleaning solution bottle is extracted and is inserted on the second cleaning solution bottle by step (a), is inverted the second cleaning solution
Cleaning solution is pumped by bottle and the second peristaltic pump of unlatching to be collected in bacterium bottle, and the second peristaltic pump is closed after cleaning solution reaches certain capacity；
Red cap stopper lid is entered to collect bacterium top of bottle by step (b), the second peristaltic pump is opened after just setting the second cleaning solution bottle, by gas
It is pumped into collection bacterium bottle and is pressurized, the cleaning to miillpore filter is realized in cleaning solution discharge from collection bacterium bottle, when cleaning solution is arranged completely
The second peristaltic pump is closed after out, removes red cap stopper to fixed position.
Full-automatic assembly line collection bacterium operating system for the present invention towards the bottled aqueous solution test sample of ampoule, including the
One manipulator, ampoule bottle box, the first hose installation auxiliary body, the second manipulator, the first peristaltic pump, surge tank/profit film liquid/clear
It is washing lotion/medium bottle box, the second peristaltic pump, hose current limliting/heat sealing mechanism, third manipulator, collection bacterium bottle rack, yellow cap stopper, red
The scrap concretes areas such as cap stopper, the 4th manipulator, hose, the 5th manipulator, the second hose installation auxiliary body, the 6th machinery
The recovery areas such as hand, collection bacterium bottle/hose/cap stopper box, the 7th manipulator, the 8th manipulator, ampoule bottle opener, reagent bottle.
First manipulator and ampoule bottle opener carry out cooperating: the first manipulator takes the peace in ampoule bottle box
Small jar bottle, and place into ampoule bottle opener；Combine with ampoule bottle opener and carries out ampoule bottle cutting, operation of breakage, it is real
Existing ampoule bottle is opened；After ampoule bottle is opened, the first manipulator puts back to ampoule bottle in ampoule bottle box；To the aqueous solution in ampoule bottle
After test sample is transferred to surge tank, ampoule bottle waste is transferred to the recovery areas such as reagent bottle by the first manipulator.
Second manipulator and the 6th manipulator carry out cooperating: the second manipulator clamp surge tank, profit film liquid bottle,
Cleaning solution bottle or medium bottle, and be put into surge tank/profit film liquid/cleaning solution/medium bottle box；The clamping of 6th manipulator and collection
The syringe needle of the connected hose one end of bacterium bottle, and insert the needle into or extract surge tank, profit film liquid bottle, cleaning solution bottle or culture medium
Bottle, the second manipulator overturn surge tank, profit film liquid bottle, cleaning solution bottle or medium bottle；When surge tank, profit film liquid bottle,
Solution in cleaning solution bottle or medium bottle be transferred to collection bacterium bottle after, the second manipulator by surge tank, profit film liquid bottle, cleaning solution bottle
Or it places in medium bottle to recovery areas such as reagent bottles.
5th manipulator, the 6th manipulator and the second hose installation auxiliary body carry out cooperating: the 6th is mechanical
Syringe needle hand clamping and collect hose one end that bacterium bottle is connected, the 5th manipulator clamping collection bacterium bottle, while stretching and movement hose will
It is put into the second peristaltic pump, and the second hose installation auxiliary body secures the hose in the second peristaltic pump.
5th manipulator, hose current limliting/heat sealing mechanism and third manipulator carry out cooperating: the 5th manipulator presss from both sides
It takes collection bacterium bottle and stretches the hose being connected with collection bacterium bottle, hose is pressed into hose current limliting/heat sealing mechanism by third manipulator；It will be soft
After pipe is packed into hose current limliting/heat sealing mechanism, the 5th manipulator will collect bacterium bottle and be put into collection bacterium bottle rack；When carry out media transfer extremely
During collecting bacterium bottle, hose current limliting/heat sealing mechanism carries out selective current limliting to hose, by media transfer to corresponding bacteria gathering device
In；After media transfer to collection bacterium bottle, hose current limliting/heat sealing mechanism will cut off the hose being connected with collection bacterium bottle, and be added
Heat or pinch seal；
5th manipulator, the 6th manipulator, hose current limliting/heat sealing mechanism and third manipulator carry out cooperating:
After the hose that hose current limliting/heat sealing mechanism cutting is connected with collection bacterium bottle, the syringe needle of the hose after the clamping cutting of the 6th manipulator,
Hose is transferred to the scrap concretes such as hose area.
7th manipulator, the 8th manipulator and the first hose installation auxiliary body carry out cooperating；7th is mechanical
Hand clamps the syringe needle of hose one end, and the 8th manipulator clamps the syringe needle of the hose other end, while stretching and mobile hose are put
Enter in the first peristaltic pump, the first hose installation auxiliary body secures the hose in the first peristaltic pump.
7th manipulator, the 8th manipulator and the second manipulator carry out cooperating: the second manipulator, which clamps, to be buffered
Tank, the 7th manipulator clamp the syringe needle of hose one end, and movement is simultaneously inserted into surge tank；The needle of the 8th manipulator clamping hose other end
Head, movement is simultaneously inserted into ampoule bottle, the aqueous solution test sample in ampoule bottle is transferred in surge tank, water-soluble in current ampoule bottle
After liquid test sample shifts, the 8th manipulator extracts syringe needle, movement and being inserted into the ampoule bottle that other have turned on carry out it is water-soluble
The transfer of liquid test sample, the aqueous solution test sample in repetitive operation to whole ampoule bottles are transferred in surge tank；To whole ampoules
After aqueous solution test sample in bottle is transferred in surge tank, the 7th manipulator extracts the syringe needle of hose one end, the 8th manipulator
The syringe needle of the hose other end is extracted, hose waste is transferred to the scrap concretes such as hose area by the 8th manipulator.
Described 8th is mechanical with the first peristaltic pump progress cooperating: when the 8th manipulator is inserted into the syringe needle of hose one end
After ampoule bottle, the first peristaltic pump is opened, carries out the transfer of aqueous solution test sample；When the aqueous solution test sample transfer in current ampoule bottle
To surge tank, the first peristaltic pump is closed；8th manipulator out of current ampoule bottle extracts the syringe needle of hose one end and be inserted into
Other have turned on ampoule bottle, open the first peristaltic pump, carry out the transfer of aqueous solution test sample；Circulate operation is until all ampoule bottles
Interior aqueous solution test sample is fully transferred in surge tank.
4th manipulator, the second peristaltic pump and the second manipulator carry out cooperating: the 4th manipulator will be from collection bacterium
Yellow cap stopper, red cap stopper are taken out in bottle/hose/cap stopper box, and are placed to fixed position；When carrying out profit film liquid, aqueous solution test sample
When being transferred to collection bacterium bottle with cleaning solution, the second manipulator is inverted surge tank, profit film liquid bottle or cleaning solution bottle, opens second and wriggles
Pump；After completion profit film liquid, aqueous solution test sample and cleaning solution are transferred to collection bacterium bottle, the second peristaltic pump, the 4th manipulator are closed
It will cover on red cap stopper to collection bacterium bottle；After covering red cap stopper, the second manipulator is just setting surge tank, profit film liquid bottle or cleaning solution
Bottle or medium bottle open the second peristaltic pump；After moistening film liquid, aqueous solution test sample and cleaning solution from collection bacterium bottle discharge, close
Second peristaltic pump, the 4th manipulator remove red cap stopper from collection bacterium bottle to fixed position；After the completion of collection bacterium bottle cleaning, the 4th
The indentation collection bacterium bottom of bottle portion of yellow cap stopper is sealed by manipulator, and the second manipulator is inverted medium bottle, opens the second peristaltic pump；
After three bottles of culture mediums are fully transferred to collection bacterium bottle, the second peristaltic pump is closed, the 4th manipulator will be covered into red cap stopper to collection bacterium bottle
On.
Furtherly, it is described collection bacterium bottle be provided with miillpore filter, it can be achieved that aqueous solution test sample filtering and Enrichment of bacteria.
Furtherly, manipulator is with the same function to grab with multivariant mechanical arm or with manipulator
Mobile mechanism.
Compared with the operation of existing manual mode, full-automation assembly line collection bacterium operating method and system proposed by the present invention,
Using string, parallel processing and flow optimization method, multiple manipulator coordinateds, the nothing of the aqueous solution test sample bottled to ampoule
Bacterium check process is standardized, medelling, and whole flow process is entirely controlled, and previous sterility test whole dependence personnel is avoided to grasp
Make, the disadvantage being as a result affected by artificial origin greatly improves the efficiency of sterility test and the accuracy rate of sterility test result
And confidence level.
Detailed description of the invention
The manual mode operating process of the bottled aqueous solution test sample of the typical ampoule of Fig. 1；
The automation collection bacterium operating process schematic diagram of the bottled aqueous solution test sample of Fig. 2 ampoule；
Modularization and time optimization processing schematic diagram of the Fig. 3 for automation collection bacterium operating process；
Fig. 4 automation collection bacterium operation system function arranges distribution schematic diagram.
Specific embodiment
Implement with reference to the accompanying drawing and most preferably to describe the present invention in detail.
Sterility test analysis towards the bottled aqueous solution test sample of ampoule, typical manual mode operating process are shown in Fig. 1.From
The operating process can be seen that the sterility test for generally carrying out three bottles or several bottles aqueous solution test samples, and operating procedure is numerous
It is more.In order to guarantee higher inspection efficiency, therefore the qualification of operator is required very high.In some cases, due to behaviour
The carelessness for making personnel can cause the false judgment of inspection result, lead to serious consequence.Therefore the present invention is proposed towards using complete
Full-automatic assembly line collection bacterium operating method and system of the automatic operation towards the bottled aqueous solution test sample of ampoule, to process into
Row is multi-level to be decomposed, and using string, parallel processing manner, modularized design completes automatic operation using manipulator coordinated.
According to versions " Chinese Pharmacopoeia " in 2015 and version GMP " pharmaceutical production quality management practices " in 2010, for ampoule bottle
Aqueous solution test sample is filled, by process optimization, automation collection bacterium operating process schematic diagram is shown in Fig. 2.Every batch of is simultaneously to 30
Operation is collecting bacteria in the bottled aqueous solution test sample of ampoule, such as according to the overall procedure of profit film-collection bacterium-cleaning-plus culture medium
Under:
(1) hose is installed and places each reagent bottle, while opening ampoule bottle；
(2) test sample passes through pipeline 2 in ampoule bottle, peristaltic pump 1 is pumped into surge tank；
(3) profit film liquid is pumped into collection bacterium bottle, each collection bacterium bottle about 50ml by pipeline 1, peristaltic pump 2；
(4) red cap stopper compresses collection bacterium bottle, and air is pumped into collection bacterium bottle by pipeline 2, peristaltic pump 2 in sample bottle, until profit film
Liquid is completely exhausted out；
(5) red cap stopper is removed, test sample is pumped into collection bacterium bottle by pipeline 2, peristaltic pump 2 from surge tank, and each collection bacterium bottle is about
100ml；
(6) red cap stopper compresses sealing collection bacterium bottle, and air is pumped into collection bacterium bottle by pipeline 2, peristaltic pump 2 in sample bottle, until
Collection bacterium filtering terminates；
(7) red cap stopper is removed, cleaning solution is pumped into collection bacterium bottle, each collection bacterium bottle about 100ml by pipeline 2, peristaltic pump 2；
(8) red cap stopper compresses sealing collection bacterium bottle, and air is pumped into collection bacterium bottle by pipeline 2, peristaltic pump 2 in cleaning solution bottle, directly
It is completely exhausted out to cleaning solution；
(9) red cap stopper is removed, collects bacterium bottom of bottle portion and is pressed into yellow cap stopper, closes B, C pipeline, culture medium 1 passes through pipeline 3, wriggling
Pump 2 is pumped into 1 about 100ml of collection bacterium bottle；
(10) A, C pipeline are closed, culture medium 2 is pumped into collection bacterium bottle 2, about 100ml by pipeline 3, peristaltic pump 2；
(11) A, B pipeline are closed, culture medium 2 is pumped into collection bacterium bottle 3, about 100ml by pipeline 3, peristaltic pump 2；
(12) red cap stopper compresses sealing collection bacterium bottle, cuts A, B, C pipeline, and seals or clamp collection bacterium bottle A, B, C pipeline；
(13) hose and each reagent bottle are removed, recovery area is moved to.
In order to shorten the operating time, improve operating efficiency, the present invention is to towards the automatic of ampoule bottle dress aqueous solution test sample
Change collection fungus strain system and carry out sub-module processing, sees Fig. 3.The system includes: packaging opening module, hose installation module 1, hose installation
Module 2, reagent bottle and cap stopper placement module, ampoule bottle opening module, reagent bottle be mobile and flip module, syringe needle plug-in module,
Cap stopper operation module, culture medium filling current limliting module, heat-sealing and shear module and hose disassembly and reagent bottle discard module.
Automation collection bacterium operation, the function of entire automation operating system are carried out using Multi-freedom-degreemanipulator manipulator coordinated
Fig. 4, its operating procedure are shown in arrangement distribution are as follows:
(1) ampoule bottle box 2, surge tank/profit film liquid/cleaning solution/medium bottle box 6, the collection bacterium bottle/soft on guide rail are clamped
Pipe/cap stopper box 18 is respectively put into fixed position.
(2) collection bacterium bottle/hose/cap stopper box 18 is opened, the hose with syringe needle being connected with collection bacterium bottle is installed compacted into second
Dynamic pump 7；The another set of hose with syringe needle in box is installed into the first peristaltic pump 5 simultaneously.
(3) hose with syringe needle being connected with collection bacterium bottle is installed into hose current limliting/heat sealing mechanism 8；It simultaneously will collection bacterium bottle
It is put into collection bacterium bottle rack 10, collects the yellow cap stopper 11 in bacterium bottle/hose/cap stopper box 18, red cap stopper 12 is put into fixed position；
(4) syringe needle being mounted on the hose in the second peristaltic pump 7 is inserted into surge tank/profit film liquid/cleaning solution/culture medium
On profit film liquid bottle in bottle box 6, the second peristaltic pump 7 is opened after being inverted profit film liquid bottle, profit film liquid is pumped into collection bacterium bottle to micropore
Filter membrane is soaked, and closes the second peristaltic pump 7 after profit film liquid reaches certain capacity；
(5) red cap stopper 12 is covered into collection bacterium top of bottle, opens the second peristaltic pump 7 after just setting profit film liquid bottle, gas is pumped into
It is pressurized in collection bacterium bottle, so that profit film liquid closes the second peristaltic pump after profit film liquid is completely exhausted out completely from collection bacterium bottle discharge
7, red cap stopper 12 is removed to fixed position；
(6) while step 2 to step 5 carries out, the ampoule bottle in ampoule bottle box 2 is sent to ampoule bottle opener
It is opened, is sent back in ampoule bottle box 2 after unlatching in 21；
(7) while step 6 carries out, the syringe needle insertion on the hose one end being mounted in the first peristaltic pump 5 has been opened
Surge tank in the ampoule bottle opened, in syringe needle insertion surge tank/profit film liquid/cleaning solution/medium bottle box 6 of the hose other end
On, it opens the first peristaltic pump 5 and the aqueous solution test sample in ampoule bottle is pumped into surge tank；To the aqueous solution in ampoule bottle for examination
After product are pumped into surge tank completely, the first peristaltic pump 5 is closed；Syringe needle is removed into the ampoule that the ampoule bottle finished has turned on to other
In bottle, opens the first peristaltic pump 5 and the aqueous solution test sample in ampoule bottle is pumped into surge tank；
It (8), will be on profit film liquid bottle after the aqueous solution test sample in the ampoule bottle of all feedings is pumped into surge tank completely
Syringe needle is extracted and is inserted on surge tank, is inverted surge tank and is opened the second peristaltic pump 7, and aqueous solution test sample is pumped into collection bacterium bottle
In, the second peristaltic pump 7 is closed after aqueous solution test sample is transferred to completely in collection bacterium bottle；
(9) red cap stopper 12 is covered into collection bacterium top of bottle, opens the second peristaltic pump 7 after just setting surge tank, gas is pumped into collection
It is pressurized in bacterium bottle, aqueous solution test sample is filtered by the miillpore filter in collection bacterium bottle and microorganism is enriched with, works as aqueous solution
Test sample closes the second peristaltic pump 7 after collection bacterium bottle discharge completely, removes red cap stopper 12 to fixed position；
(10) syringe needle on surge tank is extracted and is inserted on cleaning solution bottle, be inverted cleaning solution bottle and open the second peristaltic pump
7, cleaning solution is pumped into collection bacterium bottle, the second peristaltic pump 7 is closed after cleaning solution reaches certain capacity；
(11) red cap stopper 12 is covered into collection bacterium top of bottle, opens the second peristaltic pump 7 after just setting cleaning solution bottle, gas is pumped into
It is pressurized in collection bacterium bottle, the cleaning to miillpore filter is realized in cleaning solution discharge from collection bacterium bottle, after cleaning solution is completely exhausted out
The second peristaltic pump 7 is closed, removes red cap stopper 12 to fixed position；
(12) bottom and the indentation that yellow cap stopper 11 is moved to collection bacterium bottle, are sealed, prevent water clock；
(13) syringe needle on cleaning solution bottle is extracted and is inserted on the medium bottle equipped with a certain culture medium, simultaneously close off with
After the hose that second, third collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into first
In a collection bacterium bottle, the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
(14) syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with same medium, closed simultaneously
After closing the hose being connected with first, third collection bacterium bottle, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into
In second collection bacterium bottle, the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
(15) syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with different culture medium, closed simultaneously
After closing the hose being connected with the first, second collection bacterium bottle, it is inverted medium bottle and opens the second peristaltic pump 7, culture medium is pumped into
Third collects in bacterium bottle, and the second peristaltic pump 7 is closed after culture medium reaches certain capacity；
(16) red cap stopper 12 is covered to three hoses cut into collection bacterium top of bottle and sealed simultaneously and be connected with collection bacterium bottle.
(17) surge tank/profit film liquid/cleaning solution/medium bottle puts back to surge tank/profit film liquid/cleaning solution/medium bottle box 6
It is interior, while the hose with syringe needle on the first peristaltic pump 5 and the second peristaltic pump 7 being removed, all wastes are transferred to hose
Equal scrap concretes area 14.
(18) all devices are resetted to original state, wait the automatic collection bacterium operation of next batch aqueous solution test sample.
The Multi-freedom-degreemanipulator manipulator coordinated of use is operated:
First manipulator 1 carries out cooperating with ampoule bottle opener 25: the first manipulator 1 clamps in ampoule bottle box 2
Ampoule bottle, and place into ampoule bottle opener 25；Combine with ampoule bottle opener 25 and carries out ampoule bottle cutting, fractures
Operation realizes that ampoule bottle is opened；After ampoule bottle is opened, the first manipulator 1 puts back to ampoule bottle in ampoule bottle box 2；To ampoule bottle
After interior aqueous solution test sample is transferred to surge tank, ampoule bottle waste is transferred to the recovery areas such as reagent bottle by the first manipulator 1
22。
Second manipulator 4 and the 6th manipulator 17 carry out cooperating: the second manipulator 4 clamp surge tank, profit film liquid bottle,
Cleaning solution bottle or medium bottle, and be put into surge tank/profit film liquid/cleaning solution/medium bottle box 6；The clamping of 6th manipulator 17
The syringe needle of the hose one end being connected with collection bacterium bottle, and insert the needle into or extract surge tank, profit film liquid bottle, cleaning solution bottle or culture
Base bottle, the second manipulator 4 overturn surge tank, profit film liquid bottle, cleaning solution bottle or medium bottle；When surge tank, profit film liquid
After solution in bottle, cleaning solution bottle or medium bottle is transferred to collection bacterium bottle, the second manipulator 4 is by surge tank, profit film liquid bottle, cleaning
It places in liquid bottle or medium bottle to the recovery areas such as reagent bottle 22.
5th manipulator 15, the 6th manipulator 17 and the second hose installation auxiliary body 16 carry out cooperating: the 6th machine
Syringe needle tool hand 17 clamping and collect hose one end that bacterium bottle is connected, the 5th manipulator 15 clamping collection bacterium bottle, while stretching and movement
Hose is put it into the second peristaltic pump 7, and the second hose installation auxiliary body 16 secures the hose in the second peristaltic pump 7.
5th manipulator 15, hose current limliting/heat sealing mechanism 8 and third manipulator 9 carry out cooperating: the 5th manipulator 15
Clamping collection bacterium bottle simultaneously stretches the hose being connected with collection bacterium bottle, and hose is pressed into hose current limliting/heat sealing mechanism 8 by third manipulator 9；
After hose is packed into hose current limliting/heat sealing mechanism 8, the 5th manipulator 15 will collect bacterium bottle and be put into collection bacterium bottle rack 10.When being trained
During supporting group-transfer to collection bacterium bottle, hose current limliting/heat sealing mechanism 8 carries out selective current limliting to hose, extremely by media transfer
In corresponding bacteria gathering device；After media transfer to collection bacterium bottle, hose current limliting/heat sealing mechanism 8 will cut off and collect what bacterium bottle was connected
Hose, and carry out heating or pinch seal.
5th manipulator 15, the 6th manipulator 17, hose current limliting/heat sealing mechanism 8 carry out cooperateing with behaviour with third manipulator 9
Make: the hose after hose that hose current limliting/cutting of heat sealing mechanism 8 is connected with collection bacterium bottle, after the clamping cutting of the 6th manipulator 17
Syringe needle, hose is transferred to the scrap concretes such as hose area 14.
7th manipulator 19, the 8th manipulator 20 and the first hose installation auxiliary body 3 carry out cooperating；7th is mechanical
Hand 19 clamps the syringe needle of hose one end, and the 8th manipulator 20 clamps the syringe needle of the hose other end, while stretching and mobile hose will
It is put into the first peristaltic pump 5, and the first hose installation auxiliary body 3 secures the hose in the first peristaltic pump 5.
7th manipulator 19, the 8th manipulator 20 and the second manipulator 4 carry out cooperating: the second manipulator 4 clamps slow
Tank is rushed, the 7th manipulator 19 clamps the syringe needle of hose one end, and movement is simultaneously inserted into surge tank；It is another that 8th manipulator 20 clamps hose
The syringe needle at end, movement are simultaneously inserted into ampoule bottle, the aqueous solution test sample in ampoule bottle are transferred in surge tank, in current ampoule bottle
Aqueous solution test sample transfer after, the 8th manipulator 20 extract syringe needle, movement simultaneously be inserted into the ampoule bottle that other have turned on
The transfer of aqueous solution test sample is carried out, the aqueous solution test sample in repetitive operation to whole ampoule bottles is transferred in surge tank；To
After aqueous solution test sample in whole ampoule bottles is transferred in surge tank, the 7th manipulator 19 extracts the syringe needle of hose one end,
8th manipulator 20 extracts the syringe needle of the hose other end, and hose waste is transferred to the wastes such as hose by the 8th manipulator 20
Recovery area 14.
8th manipulator 20 and the first peristaltic pump 5 carry out cooperating: when the 8th manipulator 20 is the syringe needle of hose one end
After being inserted into ampoule bottle, the first peristaltic pump 5 is opened, carries out the transfer of aqueous solution test sample；When the aqueous solution in current ampoule bottle is for examination
After product are transferred to surge tank, the first peristaltic pump 5 is closed；8th manipulator 20 pulls out the syringe needle of hose one end out of current ampoule bottle
It out and is inserted into other and has turned on ampoule bottle, open the first peristaltic pump 5, carry out the transfer of aqueous solution test sample；Circulate operation until
Aqueous solution test sample in all ampoule bottles is fully transferred in surge tank.
4th manipulator 13, the second peristaltic pump 7 and the second manipulator 4 carry out cooperating: the 4th manipulator 13 will be from collection
Yellow cap stopper 11, red cap stopper 12 are taken out in bacterium bottle/hose/cap stopper box 18, and are placed to fixed position；When carrying out profit film liquid, water-soluble
When liquid test sample and cleaning solution are transferred to collection bacterium bottle, the second manipulator 4 is inverted surge tank, profit film liquid bottle or cleaning solution bottle, opens
Second peristaltic pump 7；After completion profit film liquid, aqueous solution test sample and cleaning solution are transferred to collection bacterium bottle, the second peristaltic pump 7 is closed,
4th manipulator 13 will cover on red cap stopper 12 to collection bacterium bottle；After covering red cap stopper 12, the second manipulator 4 just setting surge tank,
Moisten film liquid bottle or cleaning solution bottle or medium bottle, opens the second peristaltic pump 7；When profit film liquid, aqueous solution test sample and cleaning solution from
After collecting bacterium bottle discharge, the second peristaltic pump 7 is closed, the 4th manipulator 13 removes red cap stopper 12 from collection bacterium bottle to fixed position；
After the completion of collection bacterium bottle cleaning, the indentation collection bacterium bottom of bottle portion of yellow cap stopper is sealed by the 4th manipulator 13, and the second manipulator 4 falls
Medium bottle is set, the second peristaltic pump 7 is opened；After three bottles of culture mediums are fully transferred to collection bacterium bottle, the second peristaltic pump 7 is closed, the
Four manipulators 13 will cover on red cap stopper 12 to collection bacterium bottle.
Therefore each manipulator degrees-of-freedom demand in automation operating system are as follows: the first manipulator 1 needs three translations certainly
By spending, the second manipulator 4 needs three translation freedoms, a rotary freedom, and third manipulator 9 needs a translation freely
Degree, the 4th manipulator 13, the 5th manipulator 15, the 6th manipulator 17, the 7th manipulator 19 and the 8th manipulator 20 are required to three
A translation freedoms.
Claims (6)
1. the full-automatic assembly line collection bacterium operating method towards the bottled aqueous solution test sample of ampoule, it is characterised in that use string,
Parallel processing and flow optimization method, comprising the following steps:
Step (1) clamp guide rail on ampoule bottle box (2), surge tank/profit film liquid/cleaning solution/medium bottle box (6), collection bacterium bottle/
Hose/cap stopper box (18) is respectively put into fixed position；
Step (2) opens collection bacterium bottle/hose/cap stopper box (18), and the hose with syringe needle being connected with collection bacterium bottle is installed into second
Peristaltic pump (7), while the another set of hose with syringe needle in box being installed into the first peristaltic pump (5)；
Step (3) installs the hose with syringe needle being connected with collection bacterium bottle into hose current limliting/heat sealing mechanism (8)；Bacterium will be collected simultaneously
Bottle is put into collection bacterium bottle rack (10), collects the yellow cap stopper (11) in bacterium bottle/hose/cap stopper box (18), red cap stopper (12) is put into admittedly
Positioning is set；
Step (4) will be mounted on the insertion surge tank/profit film liquid/cleaning solution/culture of the syringe needle on the hose in the second peristaltic pump (7)
On profit film liquid bottle in base bottle box (6), the second peristaltic pump (7) are opened after being inverted profit film liquid bottle, profit film liquid is pumped into collection bacterium bottle
Miillpore filter is soaked, closes the second peristaltic pump (7) after profit film liquid reaches certain capacity；
Step (5) covers red cap stopper (12) into collection bacterium top of bottle, opens the second peristaltic pump after just setting profit film liquid bottle, gas is pumped into
It is pressurized in collection bacterium bottle, so that profit film liquid closes the second peristaltic pump after profit film liquid is completely exhausted out completely from collection bacterium bottle discharge,
Red cap stopper is removed to fixed position；
Ampoule bottle in ampoule bottle box is sent to ampoule bottle and opens machine by step (6) while step (2) to step (5) carries out
Structure is opened in (21), is sent back in ampoule bottle box after unlatching；
The syringe needle being mounted on hose one end in the first peristaltic pump (5) is inserted by step (7) while step (6) carry out
Buffering in the ampoule bottle having turned on, in syringe needle insertion surge tank/profit film liquid/cleaning solution/medium bottle box of the hose other end
On tank, opens the first peristaltic pump and the aqueous solution test sample in ampoule bottle is pumped into surge tank；It is supplied to the aqueous solution in ampoule bottle
After test product is pumped into surge tank completely, the first peristaltic pump is closed；Syringe needle is removed into the peace that the ampoule bottle finished has turned on to other
In small jar bottle, opens the first peristaltic pump (5) and the aqueous solution test sample in ampoule bottle is pumped into surge tank；
Step (8), will be on profit film liquid bottle after the aqueous solution test sample in the ampoule bottle of all feedings is pumped into surge tank completely
Syringe needle is extracted and is inserted on surge tank, is inverted surge tank and is opened the second peristaltic pump, and aqueous solution test sample is pumped into collection bacterium bottle,
The second peristaltic pump is closed after aqueous solution test sample is transferred to completely in collection bacterium bottle；
Red cap stopper lid is entered to collect bacterium top of bottle by step (9), opens the second peristaltic pump after just setting surge tank, and gas is pumped into collection bacterium bottle
In be pressurized, by collection bacterium bottle in miillpore filter to aqueous solution test sample filter and microorganism be enriched with, when aqueous solution for examination
Product close the second peristaltic pump after collection bacterium bottle discharge completely, remove red cap stopper to fixed position；
Syringe needle on surge tank is extracted and is inserted on cleaning solution bottle by step (10), is inverted cleaning solution bottle and is opened second and wriggles
Cleaning solution is pumped into collection bacterium bottle, the second peristaltic pump is closed after cleaning solution reaches certain capacity by pump；
Red cap stopper lid is entered to collect bacterium top of bottle by step (11), opens the second peristaltic pump after just setting cleaning solution bottle, and gas is pumped into collection
It is pressurized in bacterium bottle, the cleaning to miillpore filter is realized in cleaning solution discharge from collection bacterium bottle, is closed after cleaning solution is completely exhausted out
The second peristaltic pump is closed, removes red cap stopper to fixed position；
Yellow cap stopper (11) is moved to bottom and the indentation of collection bacterium bottle by step (12), is sealed, is prevented water clock；
Syringe needle on cleaning solution bottle is extracted and is inserted on the medium bottle equipped with a certain culture medium by step (13), simultaneously close off with
After the hose that second, third collection bacterium bottle is connected, it is inverted medium bottle and opens the second peristaltic pump, culture medium is pumped into first
Collect in bacterium bottle, the second peristaltic pump is closed after culture medium reaches certain capacity；
Syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with same medium by step (14), is closed simultaneously
It closes with first, after the hose that third collection bacterium bottle is connected, is inverted medium bottle and simultaneously opens the second peristaltic pump, culture medium is pumped into the
In two collection bacterium bottles, the second peristaltic pump is closed after culture medium reaches certain capacity；
Syringe needle on medium bottle is extracted and is inserted on another medium bottle equipped with different culture medium by step (15), is closed simultaneously
It closes with after the first, second hose for being connected of collection bacterium bottle, is inverted medium bottle and simultaneously opens the second peristaltic pump, culture medium is pumped into the
In three collection bacterium bottles, the second peristaltic pump is closed after culture medium reaches certain capacity；
Red cap stopper lid is entered to collect bacterium top of bottle by step (16), is cut and is sealed three hoses being connected with collection bacterium bottle simultaneously；
Step (17) surge tank/profit film liquid/cleaning solution/medium bottle puts back to surge tank/profit film liquid/cleaning solution/medium bottle box
(6) in, while the hose with syringe needle on the first peristaltic pump and the second peristaltic pump being removed, all wastes is transferred to hose
Equal scrap concretes area (14)；
Step (18) resets all devices to original state, waits the automatic collection bacterium operation of next batch aqueous solution test sample.
2. the full-automatic assembly line collection bacterium operation side according to claim 1 towards the bottled aqueous solution test sample of ampoule
Method, it is characterised in that: the opening time of whole ampoule bottles in ampoule bottle box (2) and whole aqueous solution test samples are transferred to buffering
The sum of time of tank should be less than being equal to peace of the hose being connected with collection bacterium bottle in the second peristaltic pump, hose current limliting/heat sealing mechanism
The sum of the wetting of miillpore filter, efflux time in ETL estimated time of loading and collection bacterium bottle.
3. the full-automatic assembly line collection bacterium operation side according to claim 1 towards the bottled aqueous solution test sample of ampoule
Method, it is characterised in that: aqueous solution test sample type to be checked need to meet the requirements the flushing dose of cleaning solution, if flushing dose is super
Single bottle cleaning solution is crossed, needs to increase by two steps afterwards in step (11):
Syringe needle on first cleaning solution bottle is extracted and is inserted on the second cleaning solution bottle by step (a), is inverted the second cleaning solution bottle simultaneously
It opens the second peristaltic pump cleaning solution is pumped into collection bacterium bottle, the second peristaltic pump is closed after cleaning solution reaches certain capacity；
Red cap stopper lid is entered to collect bacterium top of bottle by step (b), opens the second peristaltic pump after just setting the second cleaning solution bottle, gas is pumped into
It is pressurized in collection bacterium bottle, the cleaning to miillpore filter is realized in cleaning solution discharge from collection bacterium bottle, after cleaning solution is completely exhausted out
The second peristaltic pump is closed, removes red cap stopper to fixed position.
4. the full-automatic assembly line collection bacterium operating system towards the bottled aqueous solution test sample of ampoule, including the first manipulator (1),
Ampoule bottle box (2), the first hose install auxiliary body (3), the second manipulator (4), the first peristaltic pump (5), surge tank/profit film
Liquid/cleaning solution/medium bottle box (6), the second peristaltic pump (7), hose current limliting/heat sealing mechanism (8), third manipulator (9), collection bacterium
The scrap concretes areas (14), the 5th machine such as bottle rack (10), yellow cap stopper (11), red cap stopper (12), the 4th manipulator (13), hose
Tool hand (15), the second hose installation auxiliary body (16), the 6th manipulator (17), collection bacterium bottle/hose/cap stopper box (18), the 7th
The recovery areas (22) such as manipulator (19), the 8th manipulator (20), ampoule bottle opener (21), reagent bottle, it is characterised in that:
First manipulator (1) and ampoule bottle opener (21) carry out cooperating: the first manipulator (1) takes ampoule bottle box
(2) ampoule bottle in, and place into ampoule bottle opener；Combine with ampoule bottle opener and carries out ampoule bottle cutting, folding
Disconnected operation realizes that ampoule bottle is opened；After ampoule bottle is opened, the first manipulator puts back to ampoule bottle in ampoule bottle box；To ampoule bottle
After interior aqueous solution test sample is transferred to surge tank, ampoule bottle waste is transferred to the recovery areas such as reagent bottle by the first manipulator
(22)；
Second manipulator (4) and the 6th manipulator (17) carry out cooperating: the second manipulator clamps surge tank, profit film liquid
Bottle, cleaning solution bottle or medium bottle, and be put into surge tank/profit film liquid/cleaning solution/medium bottle box (6)；6th manipulator
(17) syringe needle of the hose one end being connected with collection bacterium bottle is clamped, and inserts the needle into or extract surge tank, profit film liquid bottle, cleaning solution
Bottle or medium bottle, the second manipulator overturn surge tank, profit film liquid bottle, cleaning solution bottle or medium bottle；When surge tank,
After solution in profit film liquid bottle, cleaning solution bottle or medium bottle is transferred to collection bacterium bottle, the second manipulator is by surge tank, profit film liquid
It places in bottle, cleaning solution bottle or medium bottle to recovery areas such as reagent bottles；
5th manipulator (15), the 6th manipulator (17) and the second hose installation auxiliary body (16) carry out cooperating:
The syringe needle of hose one end that the clamping of the 6th manipulator is connected with collection bacterium bottle, the 5th manipulator (15) clamping collection bacterium bottle, stretches simultaneously
It is put it into the second peristaltic pump (7) with mobile hose, the second hose installation auxiliary body secures the hose to the second peristaltic pump
In；
5th manipulator, hose current limliting/heat sealing mechanism (8) and third manipulator (9) carry out cooperating: the 5th manipulator
Clamping collection bacterium bottle simultaneously stretches the hose being connected with collection bacterium bottle, and hose is pressed into hose current limliting/heat sealing mechanism by third manipulator；It will
After hose is packed into hose current limliting/heat sealing mechanism, the 5th manipulator will collect bacterium bottle and be put into collection bacterium bottle rack (10)；When progress culture medium
It is transferred to during collection bacterium bottle, hose current limliting/heat sealing mechanism carries out selective current limliting to hose, by media transfer to corresponding
In bacteria gathering device；After media transfer to collection bacterium bottle, hose current limliting/heat sealing mechanism will cut off the hose being connected with collection bacterium bottle, and
Carry out heating or pinch seal；
5th manipulator, the 6th manipulator, hose current limliting/heat sealing mechanism and third manipulator (9) carry out cooperating: when
After the hose that hose current limliting/heat sealing mechanism cutting is connected with collection bacterium bottle, the syringe needle of the hose after the clamping cutting of the 6th manipulator will
Hose is transferred to the scrap concretes such as hose area (14)；
7th manipulator (19), the 8th manipulator (20) and the first hose installation auxiliary body (3) carry out cooperating；The
Seven manipulators clamp the syringe needle of hose one end, and the 8th manipulator clamps the syringe needle of the hose other end, while stretching and mobile hose
It puts it into the first peristaltic pump, the first hose installation auxiliary body secures the hose in the first peristaltic pump；
7th manipulator, the 8th manipulator and the second manipulator carry out cooperating: the second manipulator clamps surge tank, the
Seven manipulators clamp the syringe needle of hose one end, and movement is simultaneously inserted into surge tank；8th manipulator clamps the syringe needle of the hose other end, moves
Ampoule bottle is moved and be inserted into, the aqueous solution test sample in ampoule bottle is transferred in surge tank, the aqueous solution in current ampoule bottle supplies
After test product shifts, the 8th manipulator extracts syringe needle, movement and is inserted into progress aqueous solution confession in the ampoule bottle that other have turned on
The transfer of test product, the aqueous solution test sample in repetitive operation to whole ampoule bottles are transferred in surge tank；To in whole ampoule bottles
Aqueous solution test sample be transferred in surge tank after, the 7th manipulator extracts the syringe needle of hose one end, and the 8th manipulator will be soft
The syringe needle of the pipe other end is extracted, and hose waste is transferred to the scrap concretes such as hose area by the 8th manipulator；
Described 8th is mechanical with the first peristaltic pump progress cooperating: when the syringe needle of hose one end is inserted into ampoule by the 8th manipulator
After bottle, the first peristaltic pump is opened, carries out the transfer of aqueous solution test sample；Aqueous solution test sample in current ampoule bottle is transferred to buffering
After tank, the first peristaltic pump is closed；8th manipulator extracts the syringe needle of hose one end out of current ampoule bottle and is inserted into other
Ampoule bottle is opened, the first peristaltic pump is opened, carries out the transfer of aqueous solution test sample；Circulate operation is up to the water in all ampoule bottles
Solution test sample is fully transferred in surge tank；
4th manipulator, the second peristaltic pump and the second manipulator carry out cooperating: the 4th manipulator will be from collection bacterium bottle/soft
Yellow cap stopper, red cap stopper are taken out in pipe/cap stopper box, and are placed to fixed position；When carrying out profit film liquid, aqueous solution test sample and cleaning
When liquid is transferred to collection bacterium bottle, the second manipulator is inverted surge tank, profit film liquid bottle or cleaning solution bottle, opens the second peristaltic pump；When complete
After being transferred to collection bacterium bottle at profit film liquid, aqueous solution test sample and cleaning solution, the second peristaltic pump is closed, the 4th manipulator will be covered into red
On cap stopper to collection bacterium bottle；After covering red cap stopper, the second manipulator is just setting surge tank, profit film liquid bottle or cleaning solution bottle or culture
Base bottle opens the second peristaltic pump；After moistening film liquid, aqueous solution test sample and cleaning solution from collection bacterium bottle discharge, closes second and wriggle
Pump, the 4th manipulator remove red cap stopper from collection bacterium bottle to fixed position；After the completion of collection bacterium bottle cleaning, the 4th manipulator will
The indentation collection bacterium bottom of bottle portion of yellow cap stopper is sealed, and the second manipulator is inverted medium bottle, opens the second peristaltic pump；It is trained when three bottles
After feeding base is fully transferred to collection bacterium bottle, the second peristaltic pump is closed, the 4th manipulator will cover on red cap stopper to collection bacterium bottle.
5. the full-automatic assembly line collection bacterium according to claim 4 towards the bottled aqueous solution test sample of ampoule operates system
System, it is characterised in that: collection bacterium bottle be provided with miillpore filter, it can be achieved that aqueous solution test sample filtering and Enrichment of bacteria.
6. the full-automatic assembly line collection bacterium according to claim 5 towards the bottled aqueous solution test sample of ampoule operates system
System, it is characterised in that: manipulator is with multivariant mechanical arm or the shifting with the same function grabbed with manipulator
Motivation structure.
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| CN2237047Y (en) * | 1995-02-22 | 1996-10-09 | 叶大林 | Group bacteria cultivating device |
| CN201756550U (en) * | 2010-06-29 | 2011-03-09 | 中国人民解放军第三○二医院 | Fully-sealed bacteria collection ampoule incubator |
| CN101893589B (en) * | 2010-06-29 | 2012-10-17 | 中国人民解放军第三0二医院 | Sterility test method and totally closed bacteria collection ampoule incubator used thereby |
| CN106226151B (en) * | 2016-07-22 | 2019-03-08 | 牛刚 | Sterile cabin automatic medicine sterility test enrichment system and its operating method |
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