CN203833938U - Full-automatic microorganism detection and enrichment system - Google Patents
Full-automatic microorganism detection and enrichment system Download PDFInfo
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- CN203833938U CN203833938U CN201320890789.3U CN201320890789U CN203833938U CN 203833938 U CN203833938 U CN 203833938U CN 201320890789 U CN201320890789 U CN 201320890789U CN 203833938 U CN203833938 U CN 203833938U
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Abstract
The utility model provides a full-automatic microorganism detection and enrichment system. An industrial control machine is adopted to control a conveying device to sequentially convey a to-be-detected sample, a culture medium containing culture as well as a filter and a filter plug which are specially used for collecting bacteria and which are provided with pipelines on a preset operation station into a sterilization cabin, a middle package removing cabin, an inner package removing cabin, an enrichment operation cabin, a buffer cabin and a positive bacterium filling cabin for corresponding operation, and the microorganisms in the to-be-detected sample are enriched. The microorganisms in the to-be-detected sample are enriched in a full automatic manner, no manual operation is needed, the time and the labor are saved, false positive or false negative result caused by artificial factors can be effectively avoided, sterile full-automatic operation of the enrichment process is achieved, practical effects of accurate detection are achieved, mistakes are unlikely to make in the enrichment process, moreover the system can work continuously in the enrichment operation, the enrichment operation cabin does not need to be paused to sterilize after the enrichment operation is stopped, and thus a relatively high performance effect is achieved.
Description
Technical field
The utility model relates to microorganism detection technical field, and espespecially a kind of full automatic microorganism of realizing the automatization of microorganism detection enrichment process detects enrichment system.
Background technology
At present, microorganism detection enrichment is all manual operations, sterilization, collection bacterium drive, substratum is annotated and positive bacteria is annotated, and each unit is all independently, and positive bacteria filling environment is different from collection collarium border, complete whole requirements of process completes in different environment, the transmission of each sample to be checked between varying environment relies on and manually completes, and is very easily subject to the impact of human factor, very easily produces false positive or false-negative result.The accuracy that impact detects and ageing.
Along with the raising of people to food and drug safety requirement, the full-automatic unmanned Enrichment by Microorganisms collecting system for intervention is the inevitable demand of food and drug safety.
Utility model content
The purpose of this utility model, is to provide a kind of full automatic microorganism to detect enrichment system, can realize and treat the enrichment that sample product carry out automatization, and false positive or the false-negative result that can effectively avoid the impact due to human factor to cause, detect accurately.
For achieving the above object, the utility model is by the following technical solutions:
Full automatic microorganism detects an enrichment system, and it comprises:
One initialize operation station, this initialize operation station is in order to the filter with pipeline and the filter block head of the sample to be checked of placing, the substratum that contains culture, the special use of collection bacterium; This filter is by membrane filtration sample to be checked, the microorganism that may contain in enrichment sample to be checked;
After this initialize operation station, be adjacent to successively arrange carry out sterilizing sterilizing cabin, remove in the middle packing of packing remove cabin, the internal packing of removing internal packing removes cabin, sample to be checked is injected to filter filters enriched microorganism and by filter block head by the encapsulation of filter bottom and optionally culture is injected to the enrichment operational window of filter, the pipeline of filter that enrichment is completed is cut the buffering cabin of dress, the positive bacteria filling cabin of carrying out positive bacteria filling; Above-mentioned each cabin is connected and sealing by cabin body isolation mechanism respectively;
One in order to send into the sample to be checked on initialize operation station, substratum, filter and filter block head respectively the transport unit in corresponding above-mentioned each cabin, and this transport unit, from initialize operation station, runs through above-mentioned each cabin;
An industrial computer; This industrial computer is electrically connected to above-mentioned each cabin, each cabin body isolation mechanism and transport unit.
In a preferred embodiment, on transport unit, be provided with the stationary installation of fixing sample to be checked, the substratum that contains culture, filter and filter block head; And/or,
This transport unit is divided into device for transferring samples to be checked, substratum transport unit, filter transport unit, filter block head transport unit; And/or;
The pre-position of this transport unit in each cabin is respectively equipped with positioned sensor, and each positioned sensor is electrically connected to respectively industrial computer; And/or,
This transport unit is belt driving means or roller bearing transport unit; By the magnetic fluid being arranged in each each cabin of driven by motor out of my cabin, rotate, to drive belt or roller bearing, or transmit by motor direct-drive belt or the roller bearing being arranged in each cabin; And/or,
Respectively this cabin body isolation mechanism is separation valve door.
In a preferred embodiment, described each is respectively equipped with barometric control unit and the ventilation plant that regulates each cabin internal pressure out of my cabin; Respectively this barometric control unit and respectively this ventilation plant be electrically connected to respectively described industrial computer; Respectively in this barometric control unit, include the pressure transmitter of the corresponding cabin of sensing internal pressure, respectively this pressure transmitter is electrically connected to this industrial computer; And/or,
Described sterilizing cabin, enrichment operational window, buffering are respectively equipped with one out of my cabin and go out except the sterilising plant of bacterium in corresponding cabin.
In a preferred embodiment, described middle packing is removed cabin and described internal packing and is removed cabin and above described transport unit, be respectively equipped with middle packing removal mechanism and the internal packing removal mechanism of removing packing and internal packing in filter and filter block head; This internal packing removal mechanism and middle packing removal mechanism are electrically connected to respectively this industrial computer.
In a preferred embodiment, the described filter with pipeline is the tank body that there is respectively outlet at three two ends that are arranged in parallel, the bottom of each tank body has filter membrane, inject after sample to be checked, microorganism is by this filter membrane enrichment, the pipeline of this filter is three pipelines in parallel, and the top that one end of these three pipelines in parallel is arranged on described filter is communicated with respectively respectively this tank body, and the other end of these three pipelines in parallel is a filter syringe needle; This filter has a desired location in described enrichment operational window, and this desired location is positioned over respectively in waste liquid tank supported hole for the bottom of tank body described in each;
The inherent described transport unit of described enrichment operational window top is provided with an enrichment manipulator, this enrichment manipulator inserts in sample to be checked by the filter syringe needle of described filter and changes culture syringe needle, the culture that inserts substratum, this enrichment manipulator can make this filter move between this desired location and transport unit; This enrichment manipulator is provided with one in order to drive product to be tested or culture to inject the enrichment driving mechanism that described three pipelines in parallel enter described filter near a side in described buffering cabin;
In described enrichment operational window, near described pre-position, be provided with and filter the packaging mechanism by filter block head, each tank body bottom being sealed after enrichment;
This enrichment manipulator, enrichment driving mechanism and packaging mechanism are electrically connected to respectively this industrial computer.
In a preferred embodiment, described enrichment driving mechanism is a peristaltic pump, the pump head place of this peristaltic pump is provided with the first support and the second support, this first support and this second support clamp respectively this three pipelines in parallel when this enrichment manipulator clamps in the described sample to be checked of filter syringe needle insertion, the first support telescopically is fixed on the pump head of this peristaltic pump near described enrichment mechanical manipulator one side, this the second support telescopically and the pump head place that is arranged on this peristaltic pump that can rotate around the pump head of this peristaltic pump, this second support has a first location and a second position, this first location is arranged side by side by this first support, in order to clamp together this this three pipelines in parallel with this first support, this second position is for this second support rotation is to these three pipelines in parallel being smoothed out with the fingers to the position that can put into the pump head of this peristaltic pump along correspondence, in when operation, this first support and this second clamp respectively this three pipelines in parallel, then this second support is rotated to the second position, and this first support and this second support retrude are afterwards put into these three pipelines in parallel the pump head of described peristaltic pump,
Described enrichment manipulator also comprises one and completes after the filtration enrichment of described sample to be checked when described filter, culture can be closed to the substratum heat sealing mechanism of many two by these three pipelines in parallel before entering described filter, so culture optionally can have been injected to the filter of microorganism enrichment;
In described enrichment operational window, be also provided with three pressure transmitters of this filter internal pressure of sensing;
This peristaltic pump, this first support, this second support, this substratum heat sealing mechanism and this three pressure transmitter are electrically connected to respectively this industrial computer.
In a preferred embodiment, the top of the inherent described transport unit in described buffering cabin is provided with a pipeline and cuts mounting mechanism;
This pipeline is cut mounting mechanism and is electrically connected to described industrial computer.
In a preferred embodiment, described positive bacteria filling cabin is the positive bacteria filling cabin with heating function, above the transport unit in this positive bacteria filling cabin, is provided with positive bacteria filling mechanism; In this positive bacteria filling cabin, be also provided with temperature-control device;
This positive bacteria filling mechanism and this heating unit are electrically connected to respectively described industrial computer; And/or,
This industrial computer adopts upper computer form, and each cabin can have independent control unit.
The utility model beneficial effect is: the utility model provides a kind of full automatic microorganism to detect enrichment system, the microorganism for the treatment of in sample product carries out enrichment full-automaticly, without manual operation, time saving and energy saving, and false positive or the false-negative result that can effectively avoid the impact due to human factor to cause, realize the aseptic unattended operation of enrichment process, have and detect essence effect accurately, in enrichment process, be difficult for makeing mistakes, and can non-stop run when enrichment operates, without stopping, after enrichment operation, enrichment operational window is suspended to sterilization, there is higher implementation result.
Accompanying drawing explanation
Fig. 1 is the schematic top plan view that full automatic microorganism of the present utility model detects a preferred embodiment of enrichment system.
Fig. 2 is that the master of a preferred embodiment of full automatic microorganism detection enrichment system of the present utility model looks schematic diagram.
Fig. 3 is the schema that full automatic microorganism of the present utility model detects a preferred embodiment of enriching method.
Drawing reference numeral: 101: initialize operation station; 102: sterilizing cabin; 103: middle packing is removed cabin; 104:: internal packing removes cabin; 105: enrichment operational window; 106: buffering cabin; 107: positive bacteria filling cabin; 110: barometric control unit; 120: sterilising plant; 130: ventilation plant; 200: positioned sensor; 310: middle packing removal mechanism; 320: internal packing removal mechanism; 140: cabin body isolation mechanism; 400: transport unit; 500: enrichment driving mechanism; 510: enrichment manipulator; 520: pipeline is cut mounting mechanism; 530: packaging mechanism; 700: positive bacteria filling mechanism; 800: industrial computer; 900: three pressure transmitters; 1000: sample to be checked; 1100: filter.
Embodiment
Below only with embodiment, the enforcement aspect that the utility model is possible is described, however not in order to limit the category of the utility model institute wish protection, first give chat bright.
Shown in Fig. 1, Fig. 2, the utility model provides a kind of full automatic microorganism to detect enrichment system, and it comprises:
An initialize operation station 101, the sterilizing cabin 102 of carrying out sterilizing that is adjacent to successively to arrange after this initialize operation station 101, remove in the middle packing of packing remove cabin 103, the internal packing of removing internal packing removes cabin 104, sample to be checked is injected to filter filters enriched microorganism and by filter block head by the encapsulation of filter bottom and optionally culture is injected to the enrichment operational window 105 of filter, the pipeline of filter that enrichment is completed is cut the buffering cabin 106 of dress, the positive bacteria filling cabin 107 of carrying out positive bacteria filling.Above-mentioned each cabin is connected and sealing by cabin body isolation mechanism 140 respectively.Transport unit 400 and industrial computer 800.
This initialize operation station 101 is in order to the filter 1100 with pipeline and the filter block head of the sample to be checked 1000 of placing, the substratum that contains culture, the special use of collection bacterium; This filter is by membrane filtration sample to be checked, the microorganism that may contain in enrichment sample to be checked.
One in order to send into the sample to be checked 1000 on initialize operation station 101, substratum, filter 1100 and filter block head respectively the transport unit 400 in corresponding above-mentioned each cabin, and this transport unit 400, from initialize operation station, runs through above-mentioned each cabin;
An industrial computer 800; This industrial computer 800 is electrically connected to above-mentioned each cabin, each cabin body isolation mechanism 140 and transport unit 400, controls each cabin and moves accordingly.
As shown in Figure 3, with this utility model, can also provide full automatic microorganism enriching method, it comprises following steps:
The first step: the filter 1100 with pipeline and the filter block head of sample 1000 to be checked, the substratum that contains culture, the special use of collection bacterium are placed on the described transport unit 400 of initialize operation station;
Second step: open the cabin body isolation mechanism 140 between initialize operation station 101 and sterilizing cabin 102, filter 1100 with pipeline and the filter block head of sample 1000 to be checked, the substratum that contains culture, the special use of collection bacterium are imported in described sterilizing 102 cabins, close the cabin body isolation mechanism of 102 both sides, sterilizing cabin, carry out sterilizing, ventilation balance sterilizing cabin 102 internal pressures after sterilizing completes;
The 3rd step: open sterilizing cabin 102 and middle packing and remove the cabin body isolation mechanism between cabin 103, the filter 1100 with pipeline of sample 1000 to be checked, the substratum that contains culture, the special use of collection bacterium and filter block head are imported in described middle packing removal cabin 103, close middle packing and remove the cabin body isolation mechanism 140 of 103 both sides, cabin, carry out the middle packing of filter 1100 and air filter plug and remove;
The 4th step: open middle packing and remove the cabin body isolation mechanism 140 between cabin 103 and internal packing removal cabin 104, the filter 1100 with pipeline of sample 1000 to be checked, the substratum that contains culture, the special use of collection bacterium and filter block head are imported in described internal packing removal cabin, close internal packing and remove the cabin body isolation mechanism of both sides, cabin, carry out the internal packing of filter 1100 and air filter plug and remove;
The 5th step: open internal packing and remove the cabin body isolation mechanism 140 between cabin 104 and enrichment operational window 105, by sample 1000 to be checked, the substratum that contains culture, filter 1100 with pipeline and the filter block head of the special use of collection bacterium import in described enrichment operational window 105, microbe filter in sample 1000 to be checked is enriched in filter 1100, by filter block head, filter bottom is encapsulated again, optionally culture is injected afterwards to filter 1100, filter packaged after enrichment 1100 is placed on transport unit, obtain after filter packaged after the enrichment of respective numbers, close the cabin body isolation mechanism 140 of enrichment operational window 105 both sides,
The 6th step: open the cabin body isolation mechanism 140 between enrichment operational window 105 and buffering cabin 106, filter packaged after enrichment 1100 is imported in described buffering cabin 106, close the cabin body isolation mechanism 140 of buffering 105 both sides, cabin, the pipeline of the filter 1100 after enrichment is cut to dress;
The 7th step, open the cabin body isolation mechanism 140 between buffering cabin 106 and positive bacteria filling cabin 107, import filter packaged after enrichment 1100 into positive bacteria filling cabin 107, close the cabin body isolation mechanism 140 of positive bacteria filling 107 both sides, cabin, to by the packaged filter 1100 filling positive bacterias of cutting dress that complete after enrichment, complete the enrichment of the microorganism of test sample.
In sum, the utility model is applicable to the required enrichment operation of microorganism limit safety detection of current food and pharmaceutical security fields, and whole process is controlled by industrial computer 800, has realized automatization, has eliminated the issuable false positive of human factor or false negative.
In a preferred embodiment of the present utility model, as shown in Figure 1 and Figure 2, on transport unit 400, be provided with the stationary installation 150 of fixing sample 1000 to be checked, the substratum that contains culture, filter 1100 and filter block head, with this, can guarantee that sample 1000 to be checked, the substratum that contains culture, filter 1100 and filter block head are in the process of transmission, can thereby not misplace or topple over because of the former of inertia, and cause the pollution in each cabin and affect each cabin the accuracy while operating.
This transport unit 400 is divided into device for transferring samples to be checked, substratum transport unit, filter transport unit, filter block head transport unit, with correspondence, transmit corresponding article, reduce the complicacy of transport unit 400, for example in buffering cabin 106 and positive bacteria filling cabin 107, only need to there is filter transport unit, and do not need other three to send transport unit, save space, reduce cost.
This transport unit 400 pre-position in each cabin is respectively equipped with positioned sensor 200, and each positioned sensor 200 is electrically connected to respectively industrial computer 800.When guaranteeing transport unit 400 transmission respective articles in each cabin carry out corresponding operating time position accuracy, preferably each positioned sensor 200 is infrared sensor or correlation sensor.
Respectively this cabin body isolation mechanism 140 is separation valve door.
In a preferred embodiment of the present utility model, this transport unit 400 is belt driving means or roller bearing transport unit; By the magnetic fluid being arranged in each each cabin of driven by motor out of my cabin, rotate, to drive belt or roller bearing, or transmit by motor direct-drive belt or the roller bearing being arranged in each cabin; .
In a preferred embodiment of the present utility model, as shown in Figure 1 and Figure 2, described each is respectively equipped with barometric control unit 110 and the ventilation plant 130 that regulates each cabin internal pressure out of my cabin; Respectively this barometric control unit 110 and respectively this ventilation plant 130 be electrically connected to respectively described industrial computer; Respectively in this barometric control unit 110, include the pressure transmitter of the corresponding cabin of sensing internal pressure, respectively this pressure transmitter is electrically connected to this industrial computer.Can be between cabin and cabin with this transmission time, by the pressure in barometric control unit 110 and 130 pairs of each cabins of ventilation plant, control.Outside described sterilizing cabin 102, enrichment operational window 105, buffering cabin 106, being respectively equipped with one goes out except the sterilising plant 120 of bacterium in corresponding cabin.Can, to carrying out sterilizing in corresponding cabin, avoid polluting.
Preferably, in this preferred embodiment, in full automatic microorganism enriching method of the present utility model, initialize operation station 101, sterilizing cabin 102, middle packing are removed cabin 103, internal packing when removing cabin body isolation mechanism 140 between cabin 104, enrichment operational window 105 and opening each time, the pressure in previous cabin, lower than the pressure in a rear cabin, guarantees bacterium not to be brought into enrichment operational window 105; When the cabin body isolation mechanism 140 between enrichment operational window 105, buffering cabin 106 and positive bacteria filling cabin 107 is opened each time, the pressure in previous cabin will be higher than the pressure in a rear cabin, guarantees to be not delivered in enrichment operational window 106 positive bacteria is not reverse.
And in described the 7th step, close after the cabin body isolation mechanism 140 of positive bacteria filling 106 both sides, cabin, also comprise a step: sterilizing is carried out in described buffering cabin 106.Buffering cabin 106 has been mainly to transmit, prevent the effect that positive bacteria infects mutually.Because if enrichment operational window 105 is directly connected with positive bacteria filling cabin 107, positive bacteria may enter enrichment operational window 105.And enrichment operational window 105 is non-stop runs, if constantly carry out sterilizing meeting, lose time.So buffering cabin 106 can be connected with enrichment operational window 105 after sterilizing, does not interrupt the work of enrichment operational window 105.After buffering cabin 106 and 105 isolation of enrichment operational window, can be connected with the positive bacteria cabin 107 of annotating.Transmission is packaged after enrichment to enter behind positive bacteria filling cabin 106 after completing the filter 1100 of cutting dress, can carry out sterilizing in isolation buffer cabin 106, just can be connected with enrichment operational window 105 afterwards, and the filter 1100 carrying out next time shifts.With this, can avoid the positive bacteria in positive bacteria filling cabin 107 to enter the possibility of enrichment operational window 107, and not interrupt the operation in enrichment operation storehouse 105, make whole system there is good non-stop run.Working efficiency is high.
As shown in Figure 1 and Figure 2, in a preferred embodiment of the present utility model, described middle packing is removed cabin 103 and described internal packing and is removed cabin 104 and above described transport unit 400, be respectively equipped with and remove filter and the middle packing of filter block head and middle packing removal mechanism 310 and the internal packing removal mechanism 320 of internal packing; This internal packing removal mechanism 310 and middle packing removal mechanism 320 are electrically connected to respectively this industrial computer.
In a preferred embodiment of the present utility model, as shown in Figure 1 and Figure 2, the described filter 1100 with pipeline is the tank body that there is respectively outlet at three two ends that are arranged in parallel, and preferably this tank body is transparent.The bottom of each tank body has filter membrane, inject after sample to be checked, microorganism is by this filter membrane enrichment, the pipeline of this filter 1100 is three pipelines in parallel, the top that one end of these three pipelines in parallel is arranged on described filter 1100 is communicated with respectively respectively this tank body, and the other end of these three pipelines in parallel is a filter syringe needle; This filter 1100 has a desired location in described enrichment operational window 105, and this desired location is positioned over respectively in waste liquid tank supported hole for the bottom of tank body described in each.
The inherent described transport unit of described enrichment operational window 105 400 tops are provided with an enrichment manipulator 510, this enrichment manipulator 510 inserts in sample to be checked by the filter syringe needle of described filter 1100 and changes culture syringe needle, the culture that inserts substratum, this enrichment manipulator 510 can make this filter 1100 move between this desired location and transport unit; This enrichment manipulator 510 is provided with one in order to drive product to be tested or culture to inject the enrichment driving mechanism 500 that described three pipelines in parallel enter described filter 1100 near a side in described buffering cabin;
Described enrichment operational window 105 is interior to be provided with and to filter the packaging mechanism 530 by filter block head, each tank body bottom being sealed after enrichment near described pre-position;
This enrichment manipulator 510, enrichment driving mechanism 500 and packaging mechanism 530 are electrically connected to respectively this industrial computer 800, by this industrial computer 800, are controlled.
In a preferred embodiment of the present utility model, described enrichment driving mechanism 500 is a peristaltic pump, the pump head place of this peristaltic pump is provided with the first support and the second support, and this first support and this second support clamp respectively this three pipelines in parallel when this enrichment manipulator clamps in the described sample to be checked of filter syringe needle insertion.Wherein, the first support telescopically is fixed on the pump head of this peristaltic pump near described enrichment mechanical manipulator 510 1 sides, this second support telescopically and the pump head place that is arranged on this peristaltic pump that can rotate around the pump head of this peristaltic pump.This second support has a first location and a second position, this first location is arranged side by side by this first support, in order to clamp together this this three pipelines in parallel with this first support, this second position is for this second support rotation is to these three pipelines in parallel being smoothed out with the fingers to the position that can put into the pump head of this peristaltic pump along correspondence.In when operation, this first support and this second clamp respectively this three pipelines in parallel, then this second support is rotated to the second position, and this first support and this second support retrude are afterwards put into these three pipelines in parallel the pump head of described peristaltic pump.
Described enrichment manipulator 510 also comprises one and completes after the filtration enrichment of described sample to be checked when described filter, culture can be closed to the substratum heat sealing mechanism of many two by these three pipelines in parallel before entering described filter, so culture optionally can have been injected to the filter of microorganism enrichment.Can optionally seal one or two or all blow-by in thribble road.
In described enrichment operational window 105, be also provided with three pressure transmitters 900 of these filter 1100 internal pressures of a sensing, by the pressure in sensing filter, regulate the pressure in enrichment operational window.
This peristaltic pump, this first support, this second support, this substratum heat sealing mechanism and this three pressure transmitter 900 are electrically connected to respectively this industrial computer 800.
With this, while operating in described enrichment operational window 105, be following action:
By enrichment mechanical manipulator 510, described filter 1100 is placed on to this setting position, again strainer syringe needle is inserted and is fond of in sample 1000 afterwards, passing through the first support and the second support, three pipelines in parallel are put into the pump head of peristaltic pump, peristaltic pump is started working, liquid in sample 1000 to be checked is filtered by filter 1100, on the microorganism enrichment filter membrane that may contain, complete enrichment work.Packaging mechanism 530 seals the bottom of each tank body by filter block head afterwards, to complete encapsulation.Again by a pipeline of substratum heat sealing mechanism choosing sealing, enrichment mechanical manipulator 510 is culture syringe needle by filter needle head replacing afterwards, insert in substratum in culture, then by the effect of peristaltic pump, culture is injected to two pipelines, be enriched in two corresponding tank bodies.To sum up, in this enrichment operational window 105, complete the injection of enrichment, encapsulation and the culture of filter.
The signal that industrial computer detects according to each sensor is carried out inner sequence of control, controls respectively in the transmission of pressure in each cabin, transport unit and each cabin the corresponding actions of executive device accordingly.This sequence of control is those skilled in the art's common practise, is not that the utility model people utility model is created, therefore do not repeating at this.
With this, in a preferred embodiment of full automatic microorganism enriching method of the present utility model, described filter is three tank bodies that are arranged in parallel, can be to two tank body filling cultures wherein.
Preferably, in described the 5th step, with the sample to be checked of crossing and substratum, by transport unit 400, pass internal packing back and remove cabin, in internal packing, remove in cabin and reclaim.
Preferably, as shown in Figure 1 and Figure 2, in a preferred embodiment of the present utility model, the top of described buffering cabin 106 inherent described transport units 400 is provided with a pipeline and cuts mounting mechanism 520, to encapsulate the pipeline of this filter.
This pipeline is cut mounting mechanism 520 and is electrically connected to described industrial computer 800.
Preferably, as shown in Figure 1 and Figure 2, in a preferred embodiment of the present utility model, described positive bacteria filling cabin 107 is for having the positive bacteria filling cabin 107 of heating function, can start to cultivate after having annotated at this positive bacteria in this positive bacteria filling cabin 107.Above transport unit 400 in this positive bacteria filling cabin 107, be provided with positive bacteria filling mechanism 700; In this positive bacteria filling cabin, be also provided with temperature-control device.
This positive bacteria filling mechanism 700 and this heating unit are electrically connected to respectively described industrial computer 800.
Preferably, this industrial computer 800 adopts upper computer form, and each cabin can have independent control unit.
In sum, the utility model provides a kind of full automatic microorganism to detect enrichment system, the microorganism for the treatment of in sample product carries out enrichment full-automaticly, without manual operation, time saving and energy saving, and false positive or the false-negative result that can effectively avoid the impact due to human factor to cause, realize the aseptic unattended operation of enrichment process, have and detect essence effect accurately, in enrichment process, be difficult for makeing mistakes, and can non-stop run when enrichment operates, without stopping, after enrichment operation, enrichment operational window is suspended to sterilization, there is higher implementation result.
Claims (8)
1. full automatic microorganism detects an enrichment system, it is characterized in that, it comprises:
One initialize operation station, this initialize operation station is in order to the filter with pipeline and the filter block head of the sample to be checked of placing, the substratum that contains culture, the special use of collection bacterium; This filter is by membrane filtration sample to be checked, the microorganism that may contain in enrichment sample to be checked;
After this initialize operation station, be adjacent to successively arrange carry out sterilizing sterilizing cabin, remove in the middle packing of packing remove cabin, the internal packing of removing internal packing removes cabin, sample to be checked is injected to filter filters enriched microorganism and by filter block head by the encapsulation of filter bottom and optionally culture is injected to the enrichment operational window of filter, the pipeline of filter that enrichment is completed is cut the buffering cabin of dress, the positive bacteria filling cabin of carrying out positive bacteria filling; Above-mentioned each cabin is connected and sealing by cabin body isolation mechanism respectively;
One in order to send into the sample to be checked on initialize operation station, substratum, filter and filter block head respectively the transport unit in corresponding above-mentioned each cabin, and this transport unit, from initialize operation station, runs through above-mentioned each cabin;
An industrial computer; This industrial computer is electrically connected to above-mentioned each cabin, each cabin body isolation mechanism and transport unit.
2. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, is provided with the stationary installation of fixing sample to be checked, the substratum that contains culture, filter and filter block head on transport unit; And/or,
This transport unit is divided into device for transferring samples to be checked, substratum transport unit, filter transport unit, filter block head transport unit; And/or;
The pre-position of this transport unit in each cabin is respectively equipped with positioned sensor, and each positioned sensor is electrically connected to respectively industrial computer; And/or,
This transport unit is belt driving means or roller bearing transport unit; By the magnetic fluid being arranged in each each cabin of driven by motor out of my cabin, rotate, to drive belt or roller bearing, or transmit by motor direct-drive belt or the roller bearing being arranged in each cabin.
3. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, described each is respectively equipped with barometric control unit and the ventilation plant that regulates each cabin internal pressure out of my cabin; Respectively this barometric control unit and respectively this ventilation plant be electrically connected to respectively described industrial computer; Respectively in this barometric control unit, include the pressure transmitter of the corresponding cabin of sensing internal pressure, respectively this pressure transmitter is electrically connected to this industrial computer; And/or,
Described sterilizing cabin, enrichment operational window, buffering are respectively equipped with one out of my cabin and go out except the sterilising plant of bacterium in corresponding cabin.
4. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, described middle packing is removed cabin and described internal packing and is removed cabin and above described transport unit, be respectively equipped with middle packing removal mechanism and the internal packing removal mechanism of removing packing and internal packing in filter and filter block head; This internal packing removal mechanism and middle packing removal mechanism are electrically connected to respectively this industrial computer.
5. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, the described filter with pipeline is the tank body that there is respectively outlet at three two ends that are arranged in parallel, the bottom of each tank body has filter membrane, inject after sample to be checked, microorganism is by this filter membrane enrichment, and the pipeline of this filter is three pipelines in parallel, the top that one end of these three pipelines in parallel is arranged on described filter is communicated with respectively respectively this tank body, and the other end of these three pipelines in parallel is a filter syringe needle; This filter has a desired location in described enrichment operational window, and this desired location is positioned over respectively in waste liquid tank supported hole for the bottom of tank body described in each;
The inherent described transport unit of described enrichment operational window top is provided with an enrichment manipulator, this enrichment manipulator inserts in sample to be checked by the filter syringe needle of described filter and changes culture syringe needle, the culture that inserts substratum, this enrichment manipulator can make this filter move between this desired location and transport unit; This enrichment manipulator is provided with one in order to drive product to be tested or culture to inject the enrichment driving mechanism that described three pipelines in parallel enter described filter near a side in described buffering cabin;
In described enrichment operational window, near described pre-position, be provided with and filter the packaging mechanism by filter block head, each tank body bottom being sealed after enrichment;
This enrichment manipulator, enrichment driving mechanism and packaging mechanism are electrically connected to respectively this industrial computer.
6. full automatic microorganism according to claim 5 detects enrichment system, it is characterized in that, described enrichment driving mechanism is a peristaltic pump, the pump head place of this peristaltic pump is provided with the first support and the second support, this first support and this second support clamp respectively this three pipelines in parallel when this enrichment manipulator clamps in the described sample to be checked of filter syringe needle insertion, the first support telescopically is fixed on the pump head of this peristaltic pump near described enrichment mechanical manipulator one side, this the second support telescopically and the pump head place that is arranged on this peristaltic pump that can rotate around the pump head of this peristaltic pump, this second support has a first location and a second position, this first location is arranged side by side by this first support, in order to clamp together this this three pipelines in parallel with this first support, this second position is for this second support rotation is to these three pipelines in parallel being smoothed out with the fingers to the position that can put into the pump head of this peristaltic pump along correspondence, in when operation, this first support and this second clamp respectively this three pipelines in parallel, then this second support is rotated to the second position, and this first support and this second support retrude are afterwards put into these three pipelines in parallel the pump head of described peristaltic pump,
Described enrichment manipulator also comprises one and completes after the filtration enrichment of described sample to be checked when described filter, culture can be closed to the substratum heat sealing mechanism of many two by these three pipelines in parallel before entering described filter, so culture optionally can have been injected to the filter of microorganism enrichment;
In described enrichment operational window, be also provided with three pressure transmitters of this filter internal pressure of sensing;
This peristaltic pump, this first support, this second support, this substratum heat sealing mechanism and this three pressure transmitter are electrically connected to respectively this industrial computer.
7. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, the top of the inherent described transport unit in described buffering cabin is provided with a pipeline and cuts mounting mechanism;
This pipeline is cut mounting mechanism and is electrically connected to described industrial computer.
8. full automatic microorganism according to claim 1 detects enrichment system, it is characterized in that, described positive bacteria filling cabin is the positive bacteria filling cabin with heating function, above the transport unit in this positive bacteria filling cabin, is provided with positive bacteria filling mechanism; In this positive bacteria filling cabin, be also provided with temperature-control device;
This positive bacteria filling mechanism and this heating unit are electrically connected to respectively described industrial computer; And/or,
This industrial computer adopts upper computer form, and each cabin can have independent control unit.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320890789.3U CN203833938U (en) | 2013-12-31 | 2013-12-31 | Full-automatic microorganism detection and enrichment system |
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| CN201320890789.3U CN203833938U (en) | 2013-12-31 | 2013-12-31 | Full-automatic microorganism detection and enrichment system |
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| CN203833938U true CN203833938U (en) | 2014-09-17 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745464A (en) * | 2013-12-31 | 2015-07-01 | 牛刚 | Full-automatic microbiological detection enrichment system and enrichment method thereof |
| CN117025377A (en) * | 2023-08-15 | 2023-11-10 | 瑞阳制药股份有限公司 | Sterile automatic inspection equipment and method for finished injection medicine |
-
2013
- 2013-12-31 CN CN201320890789.3U patent/CN203833938U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104745464A (en) * | 2013-12-31 | 2015-07-01 | 牛刚 | Full-automatic microbiological detection enrichment system and enrichment method thereof |
| WO2015101326A1 (en) * | 2013-12-31 | 2015-07-09 | 牛刚 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| CN104745464B (en) * | 2013-12-31 | 2016-09-14 | 牛刚 | Full automatic microorganism detection enrichment system and enrichment method thereof |
| JP2017502696A (en) * | 2013-12-31 | 2017-01-26 | ニョ， ガンＮＩＵ， Ｇａｎｇ | Fully automatic microbial detection integration system and its integration method |
| CN117025377A (en) * | 2023-08-15 | 2023-11-10 | 瑞阳制药股份有限公司 | Sterile automatic inspection equipment and method for finished injection medicine |
| CN117025377B (en) * | 2023-08-15 | 2024-04-23 | 瑞阳制药股份有限公司 | Sterile automatic inspection equipment and method for finished injection medicine |
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