CN104745464B - Full automatic microorganism detection enrichment system and enrichment method thereof - Google Patents
Full automatic microorganism detection enrichment system and enrichment method thereof Download PDFInfo
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- CN104745464B CN104745464B CN201310751864.2A CN201310751864A CN104745464B CN 104745464 B CN104745464 B CN 104745464B CN 201310751864 A CN201310751864 A CN 201310751864A CN 104745464 B CN104745464 B CN 104745464B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/44—Multiple separable units; Modules
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/18—Rollers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The present invention provides a kind of full automatic microorganism detection enrichment system and enrichment method thereof, transporter is controlled by the filter with pipeline special to the measuring samples on a preset operation position, culture medium containing culture, collection bacterium and filter block head by industrial computer, the most incoming sterilizing cabin, outer package remove in cabin, inner packing removing cabin, enrichment procedure cabin, buffering cabin, positive bacteria filling cabin to operate accordingly, are enriched with the microorganism in measuring samples.Reached the microorganism in measuring samples to be enriched with full-automaticly, without manual operation, time saving and energy saving, and the false positive caused due to the impact of anthropic factor or false-negative result can be prevented effectively from, it is achieved and the aseptic full automatic working of enrichment process, have and detect substantial effect accurately, in enrichment process the most error-prone, and can work continuously when enrichment procedure, it is not necessary to after stopping enrichment procedure, sterilization is suspended in enrichment procedure cabin, there is higher implementation result.
Description
Technical field
The present invention relates to technical field of microbial detection, a kind of enrichment process realizing microorganism detection from
The full automatic microorganism detection enrichment system of dynamicization and enrichment method thereof.
Background technology
At present, microorganism detection enrichment is entirely manual operations, and sterilization, collection bacterium drive, culture medium
Filling and positive bacteria filling each unit are all independent, and positive bacteria filling environment is different from collection collarium border
, i.e. completing whole requirements of process and complete in different environment, each measuring samples is between varying environment
Transmission dependence is accomplished manually, and is highly prone to the impact of anthropic factor, easily produces false positive or false-negative knot
Really.The accuracy of impact detection is with ageing.
Raising food and drug safety required along with people, the full-automatic unmanned microorganism enrichment for intervening
System is the necessity of food and drug safety.
Summary of the invention
The purpose of the present invention, is to provide a kind of full automatic microorganism detection enrichment system and enrichment method, energy
Enough enrichments realizing measuring samples carries out automatization, can be prevented effectively from the false sun caused due to the impact of anthropic factor
Property or false-negative result, detection is accurately.
For achieving the above object, the present invention is by the following technical solutions:
A kind of full automatic microorganism detects enrichment system, and it comprises:
One preset operation position, this preset operation position in order to the measuring samples placed, culture medium containing culture,
Collect the special filter with pipeline of bacterium and filter block head；This filter passes through membrane filtration measuring samples, is enriched with to be checked
The microorganism that may contain in sample；
Be disposed adjacently successively behind this preset operation position carry out sterilizing sterilizing cabin, remove outer package outer package remove
Go to cabin, the inner packing removing cabin of removal inner packing, measuring samples is injected filter filtration enriched microorganism and passes through filter
Block head is by the encapsulation of filter bottom and culture optionally injects the enrichment procedure cabin of filter, to be enriched with
The pipeline of filter carries out cutting the buffering cabin of dress, carrying out the positive bacteria filling cabin of positive bacteria filling；Above-mentioned each cabin is passed through respectively
Nacelle interrupter is connected and closes；
One in order to be respectively fed to phase by the measuring samples on preset operation position, culture medium, filter and filter block head
Answering the transporter in above-mentioned each cabin, this transporter, from the beginning of preset operation position, runs through above-mentioned each cabin；
One industrial computer；This industrial computer electrically connects above-mentioned each cabin, each nacelle interrupter and transporter.
In a preferred embodiment, transporter is provided with fixing measuring samples, the culture medium containing culture, filter
Device and the fixing device of filter block head；And/or,
This transporter is divided into measuring samples transporter, culture medium transporter, filter transporter, filter to block
Head transporter；And/or；
This transporter pre-position in each cabin is respectively equipped with alignment sensor, and each alignment sensor is electrically connected respectively
Connect industrial computer；And/or,
This transporter is belt conveyer or roller bearing transporter；By being arranged on each each cabin of driven by motor out of my cabin
In magnetic fluid rotate, to drive belt or roller bearing, or by the motor direct-drive belt being arranged in each cabin or
Roller bearing transmission；And/or,
Respectively this nacelle interrupter is separation valve door.
In a preferred embodiment, described each barometric control unit and the ventilation regulating pressure in each cabin it is respectively equipped with the most out of my cabin
Device；Respectively this barometric control unit and respectively this ventilation unit be electrically connected described industrial computer；Respectively this barometric control unit
Inside including and sense the pressure transducer of pressure in corresponding cabin, respectively this pressure transducer electrically connects this industrial computer；And/or,
Described sterilizing cabin, enrichment procedure cabin, buffering are respectively equipped with one out of my cabin and go out except the bactericidal unit of antibacterial in corresponding cabin.
In a preferred embodiment, described outer package removes cabin and described inner packing removing cabin above described transporter
It is respectively equipped with removal filter and filter block head outer package and the outer package removal mechanism of inner packing and inner packing removes machine
Structure；This inner packing removal mechanism and outer package removal mechanism are electrically connected this industrial computer.
In a preferred embodiment, the described filter with pipeline is the tank that three two ends being arranged in parallel have outlet respectively
Body, the bottom of each tank body has filter membrane, and after injecting measuring samples, microorganism is enriched with by this filter membrane, the pipe of this filter
Lu Weisan pipeline in parallel, one end of these three pipelines in parallel is arranged on the top of described filter and is respectively communicated with and respectively should
Tank body, the other end of these three pipelines in parallel is a filter syringe needle；This filter has one in described enrichment procedure cabin
Setting position, this setting position is that the bottom of each described tank body is respectively placed in waste liquid tank supported hole；
It is arranged over an enrichment procedure mechanical hand at described transporter, this enrichment procedure machine in described enrichment procedure cabin
The filter syringe needle of described filter is inserted in measuring samples and changes culture syringe needle by tool hands, inserts the cultivation of culture medium
Thing, this enrichment procedure mechanical hand can make this filter move between this setting position and transporter；This enrichment procedure machine
Tool hands is provided with one in order to drive product to be tested or culture to inject described three parallel connections near the side in described buffering cabin
Pipeline in enter the enrichment drive mechanism of described filter；
After described pre-position is provided with filtration enrichment, filter block head is passed through to each tank in described enrichment procedure cabin
Body bottom carries out the packaging mechanism closed；
This enrichment procedure mechanical hand, enrichment drive mechanism and packaging mechanism are electrically connected this industrial computer.
In a preferred embodiment, described enrichment drive mechanism is a peristaltic pump, is provided with first at the pump head of this peristaltic pump
Support and the second support, this first support and this second support insert institute at this enrichment procedure manipulator clamping filter syringe needle
Clamping this three pipelines in parallel when stating in measuring samples respectively, the first support is telescopically fixed on the pump of this peristaltic pump
The nearly described enrichment mechanical hand side of head rest, this second support telescopically and can around this peristaltic pump pump head rotate setting
Putting at the pump head of this peristaltic pump, this second support has a primary importance and a second position, and this primary importance is side by side
Being arranged on by this first support, in order to clamp this three pipelines in parallel together with this first support, this second position is
This second support rotates to the position smoothed out with the fingers by these three pipelines in parallel in the pump head that can put into this peristaltic pump along correspondence；?
During operation, this first support clamps this three pipelines in parallel respectively with this second, then is rotated extremely by this second support
The second position, these three pipelines in parallel are put into described peristaltic pump by this first support and this second support retraction afterwards
Pump head in；
Described enrichment procedure mechanical hand also comprise one when described filter complete described measuring samples filtration enrichment after, cultivate
Thing enters the culture medium heat sealing mechanism that these three pipelines in parallel can be closed before described filter at most two, so can be by
Culture has optionally injected in the filter of microorganism enrichment；
Three pressure transducers sensing this filter internal pressure it are additionally provided with in described enrichment procedure cabin；
This peristaltic pump, this first support, this second support, this culture medium heat sealing mechanism and this three pressure transducer divide
Do not electrically connect this industrial computer.
In a preferred embodiment, in described buffering cabin, the pipeline that is arranged at described transporter cuts installation
Structure；
This pipeline is cut mounting mechanism and is electrically connected described industrial computer.
In a preferred embodiment, described positive bacteria filling cabin is the positive bacteria filling cabin with heating function, at this sun
Property bacterium filling cabin in transporter be arranged over positive bacteria filling mechanism；It is additionally provided with temperature in this positive bacteria filling cabin
Control device；
This positive bacteria filling mechanism and this heater are electrically connected described industrial computer；And/or,
This industrial computer uses host computer form, and each cabin can have independent control unit.
A kind of detection method applying any of the above-described full automatic microorganism detection enrichment system, it comprises the steps of
The first step: by the filter with pipeline special to measuring samples, culture medium containing culture, collection bacterium and filter
Block head is placed on the described transporter of preset operation position；
Second step: open the nacelle interrupter between preset operation position and sterilizing cabin, by measuring samples, containing training
Supporting the culture medium of thing, collect in the special filter with pipeline of bacterium and the incoming described sterilizing cabin of filter block head, closedown is gone out
The nacelle interrupter of both sides, bacterium cabin, carries out sterilizing, and after sterilizing completes, ventilation balances pressure in sterilizing cabin；
3rd step: open sterilizing cabin and the nacelle interrupter between cabin is removed in outer package, by measuring samples, containing training
Support the culture medium of thing, collect in the special filter with pipeline of bacterium and filter block head incoming described outer package removal cabin,
Closing outer package and remove the nacelle interrupter of both sides, cabin, the outer package carrying out filter and filter block head is removed；
4th step: open outer package and remove cabin and inner packing and remove the nacelle interrupter between cabin, by measuring samples,
The filter with pipeline and the incoming described inner packing of filter block head that culture medium containing culture, collection bacterium are special are removed
In cabin, closing inner packing and remove the nacelle interrupter of both sides, cabin, the inner packing carrying out filter and filter block head is removed；
5th step: open inner packing and remove the nacelle interrupter between cabin and enrichment procedure cabin, by measuring samples, contain
There is the culture medium of culture, collect the special filter with pipeline of bacterium and the incoming described enrichment procedure cabin of filter block head
In, the microbe filter in measuring samples is enriched in filter, then by filter block head, filter bottom is encapsulated,
The most optionally culture is injected filter, be placed on being enriched with the good filter of post package on transporter, it is thus achieved that phase
After answering the filter that the enrichment post package of quantity is good, close the nacelle interrupter of both sides, enrichment procedure cabin；
6th step: open the nacelle interrupter between enrichment procedure cabin and buffering cabin, the filter good by being enriched with post package
In incoming described buffering cabin, close the nacelle interrupter of buffering both sides, cabin, the pipeline of the filter good to enrichment post package
Carry out cutting dress；
7th step, opens the nacelle interrupter between buffering cabin and positive bacteria filling cabin, enrichment post package is completed well
Cut the filter incoming positive bacteria filling cabin of dress, close the nacelle interrupter of positive bacteria filling both sides, cabin, after being enriched with
The packaged filter filling positive bacteria completing to cut dress, completes the enrichment of the microorganism of test sample.
In a preferred embodiment, preset operation position, sterilizing cabin, outer package remove cabin, inner packing removing cabin, richness
When nacelle interrupter between collection operational window is opened each time, the pressure in previous cabin is less than the pressure in later cabin,
Ensure antibacterial is not brought into enrichment procedure cabin；Nacelle isolation between enrichment procedure cabin, buffering cabin and positive bacteria filling cabin
When mechanism opens each time, the pressure in previous cabin is higher than the pressure in later cabin, it is ensured that positive bacteria is the most not reverse
It is delivered in enrichment procedure cabin；And/or,
In described 7th step, after closing the nacelle interrupter of positive bacteria filling both sides, cabin, also comprise a step: to institute
State buffering cabin and carry out sterilizing；And/or,
Described filter is the tank body that three two ends being arranged in parallel have outlet respectively, optionally to the most at most two
Tank body filling culture；And/or,
In described 5th step, used measuring samples and culture medium are passed inner packing back by transporter and are removed cabin,
Inner packing is removed in cabin and is reclaimed.
The medicine have the advantages that the present invention provides a kind of full automatic microorganism detection enrichment system and method thereof, entirely
Automatically the microorganism in measuring samples is enriched with, it is not necessary to manual operation, time saving and energy saving, and can be prevented effectively from
The false positive caused due to the impact of anthropic factor or false-negative result, it is achieved the aseptic full-automatic behaviour of enrichment process
Make, have and detect substantial effect accurately, the most error-prone in enrichment process, and can work continuously when enrichment procedure,
Without sterilization being suspended in enrichment procedure cabin after stopping enrichment procedure, there is higher implementation result.
Accompanying drawing explanation
Fig. 1 is the schematic top plan view of a preferred embodiment of the full automatic microorganism detection enrichment system of the present invention.
Fig. 2 is the schematic front view of a preferred embodiment of the full automatic microorganism detection enrichment system of the present invention.
Fig. 3 is the flow chart of a preferred embodiment of the full automatic microorganism detection enrichment method of the present invention.
Drawing reference numeral: 101: preset operation position；102: sterilizing cabin；103: outer package removes cabin；104:
Inner packing removing cabin；105: enrichment procedure cabin；106: buffering cabin；107: positive bacteria filling cabin；110:
Barometric control unit；120: bactericidal unit；130: ventilation unit；200: alignment sensor；310: outer
Packaging removal mechanism；320: inner packing removal mechanism；140: nacelle interrupter；400: transporter；
500: enrichment drive mechanism；510: enrichment procedure mechanical hand；520: pipeline cuts mounting mechanism；530: encapsulation
Mechanism；700: positive bacteria fills mechanism；800: industrial computer；900: three pressure transducers；1000:
Measuring samples；1100: filter.
Detailed description of the invention
Hereinafter only with embodiment, the enforcement aspect that the present invention is possible is described, but and is not used to limit institute of the present invention
The category to be protected, first give chat bright.
Shown in Fig. 1, Fig. 2, the present invention provides a kind of full automatic microorganism detection enrichment system, and it comprises:
One preset operation position 101, be disposed adjacently successively behind this preset operation position 101 carries out sterilizing
Sterilizing cabin 102, remove outer package outer package remove cabin 103, remove inner packing inner packing removing cabin 104, will
Measuring samples inject filter filter enriched microorganism and by filter block head by filter bottom encapsulate and optionally
Culture is injected the enrichment procedure cabin 105 of filter, the pipeline of the filter being enriched with is cut the buffering cabin of dress
106, the positive bacteria filling cabin 107 of positive bacteria filling is carried out.Above-mentioned each cabin is respectively by nacelle interrupter 140 phase
Even with closing.Transporter 400 and industrial computer 800.
This preset operation position 101 is special in order to the measuring samples 1000 placed, culture medium containing culture, collection bacterium
The filter 1100 with pipeline and filter block head；This filter passes through membrane filtration measuring samples, is enriched with to be checked
The microorganism that may contain in sample.
One in order to by the measuring samples 1000 on preset operation position 101, culture medium, filter 1100 and filter
Block head is respectively fed to the transporter 400 in corresponding above-mentioned each cabin, and this transporter 400 is opened from preset operation position
Begin, run through above-mentioned each cabin；
One industrial computer 800；This industrial computer 800 electrically connects above-mentioned each cabin, each nacelle interrupter 140 and transmits
Device 400, controls each cabin and carries out corresponding action.
As it is shown on figure 3, may be provided for full automatic microorganism enrichment method with this present invention, it comprises the steps of
The first step: by the filter with pipeline special to measuring samples 1000, culture medium containing culture, collection bacterium
1100 and filter block head be placed on the described transporter 400 of preset operation position；
Second step: open the nacelle interrupter 140 between preset operation position 101 and sterilizing cabin 102, will treat
The filter 1100 with pipeline that sample product 1000, culture medium containing culture, collection bacterium are special and filter block head
In incoming described sterilizing 102 cabin, closing the nacelle interrupter of both sides, sterilizing cabin 102, carry out sterilizing, sterilizing is complete
Pressure in ventilation balance sterilizing cabin 102 after one-tenth；
3rd step: open sterilizing cabin 102 and the nacelle interrupter between cabin 103 is removed in outer package, sample will be treated
The filter 1100 with pipeline and filter block head that product 1000, culture medium containing culture, collection bacterium are special are incoming
Described outer package is removed in cabin 103, closes outer package and removes the nacelle interrupter 140 of both sides, cabin 103, carries out
The outer package of filter 1100 and filter block head is removed；
4th step: open outer package and remove the nacelle interrupter 140 between cabin 103 and inner packing removal cabin 104,
By the filter 1100 with pipeline special to measuring samples 1000, culture medium containing culture, collection bacterium and filter envelope
The incoming described inner packing of plug is removed in cabin, closes inner packing and removes the nacelle interrupter of both sides, cabin, carries out filter
1100 and filter block head inner packing remove；
5th step: open inner packing and remove the nacelle interrupter 140 between cabin 104 and enrichment procedure cabin 105,
By the filter 1100 with pipeline special to measuring samples 1000, culture medium containing culture, collection bacterium and filter envelope
In the incoming described enrichment procedure cabin 105 of plug, the microbe filter in measuring samples 1000 is enriched in filter 1100
In, then by filter block head, filter bottom is encapsulated, the most optionally culture is injected filter 1100, will
The enrichment good filter 1100 of post package is placed on transporter, it is thus achieved that the filter that the enrichment post package of respective numbers is good
After, close the nacelle interrupter 140 of both sides, enrichment procedure cabin 105；
6th step: open the nacelle interrupter 140 between enrichment procedure cabin 105 and buffering cabin 106, will enrichment
In the incoming described buffering cabin 106 of filter 1100 that post package is good, close the nacelle interrupter of buffering both sides, cabin 105
140, the pipeline of the filter 1100 after enrichment is cut dress；
7th step, opens the nacelle interrupter 140 between buffering cabin 106 and positive bacteria filling cabin 107, by richness
The filter 1100 incoming positive bacteria filling cabin 107 that collection post package is good, closes the nacelle of positive bacteria filling both sides, cabin 107
Interrupter 140, fills positive bacteria to the filter 1100 that enrichment post package completes well to cut dress, completes test sample
The enrichment of microorganism.
In sum, needed for the present invention is applicable to the microorganism limiting safe detection in current food and drug safety field
Enrichment procedure, the omnidistance industrial computer 800 that passes through controls, it is achieved that automatization, eliminates the issuable vacation of anthropic factor
The positive or false negative.
In a preferred embodiment of the present invention, as shown in Figure 1 and Figure 2, transporter 400 is provided with fixing treating
Sample product 1000, the fixing device 150 of culture medium, filter 1100 and filter block head containing culture, with this
Can ensure that measuring samples 1000, culture medium, filter 1100 and filter block head containing culture are in the process of transmission
In, will not misplace because of the reason of inertia or topple over, and when causing the pollution in each cabin and affect the operation of each cabin
Accuracy.
This transporter 400 is divided into measuring samples transporter, culture medium transporter, filter transporter, filter
Block head transporter, transmits corresponding article with correspondence, reduces the complexity of transporter 400, such as in buffering
Have only to filter transporter in cabin 106 and positive bacteria filling cabin 107, send transmission dress without other three
Put, save space, reduce cost.
This transporter 400 pre-position in each cabin is respectively equipped with alignment sensor 200, each alignment sensor
200 are electrically connected industrial computer 800.During to ensure that transporter 400 transmits, respective articles carries out phase in each cabin
The accuracy of position when should operate, the most each alignment sensor 200 is infrared ray sensor or opposite type sensor.
Respectively this nacelle interrupter 140 is separation valve door.
In a preferred embodiment of the present invention, this transporter 400 is belt conveyer or roller bearing transporter；
Rotated by the magnetic fluid being arranged in each each cabin of driven by motor out of my cabin, to drive belt or roller bearing, or by setting
Put the motor direct-drive belt in each cabin or roller bearing transmission.
In a preferred embodiment of the present invention, as shown in Figure 1 and Figure 2, described each being respectively equipped with out of my cabin regulates each cabin
The barometric control unit 110 of interior pressure and ventilation unit 130；Respectively this barometric control unit 110 and respectively this ventilation dress
Put 130 and be electrically connected described industrial computer；Respectively include pressure in the corresponding cabin of sensing in this barometric control unit 110
Pressure transducer, respectively this pressure transducer electrically connects this industrial computer.With this can transmission between cabin and cabin time, logical
Cross barometric control unit 110 and the pressure in each cabin is controlled by ventilation unit 130.Described sterilizing cabin 102, richness
It is respectively equipped with one outside collection operational window 105, buffering cabin 106 to go out except the bactericidal unit 120 of antibacterial in corresponding cabin.Can be right
Sterilizing is carried out, it is to avoid pollute in corresponding cabin.
It is preferred that in this preferred embodiment, in the full automatic microorganism enrichment method of the present invention, preset operation position
101, sterilizing cabin 102, outer package remove cabin 103, inner packing removing between cabin 104, enrichment procedure cabin 105
When nacelle interrupter 140 is opened each time, the pressure in previous cabin is less than the pressure in later cabin, it is ensured that will be thinless
Cingula enters enrichment procedure cabin 105；Cabin between enrichment procedure cabin 105, buffering cabin 106 and positive bacteria filling cabin 107
When body interrupter 140 is opened each time, the pressure in previous cabin is higher than the pressure in later cabin, it is ensured that not by sun
Bacterium is the most inversely delivered in enrichment procedure cabin 106 property.
And in described 7th step, after closing the nacelle interrupter 140 of positive bacteria filling both sides, cabin 106, also comprise
One step: described buffering cabin 106 is carried out sterilizing.Buffering cabin 106 has been mainly transmission, has prevented positive bacteria mutual
The effect infected.Because if enrichment procedure cabin 105 is joined directly together with positive bacteria filling cabin 107, positive bacteria may
Enter enrichment procedure cabin 105.And enrichment procedure cabin 105 is continuously work, if constantly carrying out sterilizing when can waste
Between.So buffering cabin 106 can be connected with enrichment procedure cabin 105 after sterilization, does not interrupt enrichment procedure cabin 105
Work.After buffering cabin 106 isolates with enrichment procedure cabin 105, can be connected with positive bacteria filling cabin 107.
After transmission enrichment post package enters positive bacteria filling cabin 106 after completing well to cut the filter 1100 of dress, get final product isolation buffer
Cabin 106 carries out sterilizing, the most just can be connected with enrichment procedure cabin 105, carries out filter 1100 next time and shifts.
The positive bacteria in positive bacteria filling cabin 107 can be avoided to enter the possibility in enrichment procedure cabin 107 with this, and not interrupt richness
The operation in collection operation storehouse 105, makes whole system have workability the most continuously.Work efficiency is high.
As shown in Figure 1 and Figure 2, in a preferred embodiment of the present invention, described outer package removes cabin 103 and described
Inner packing removing cabin 104 is respectively equipped with removal filter and the outsourcing of filter block head above described transporter 400
Fill the outer package removal mechanism 310 with inner packing and inner packing removal mechanism 320；This inner packing removal mechanism 310
It is electrically connected this industrial computer with outer package removal mechanism 320.
In a preferred embodiment of the present invention, as shown in Figure 1 and Figure 2, the described filter 1100 with pipeline is
Three two ends being arranged in parallel have the tank body of outlet respectively, and preferably this tank body is transparent.The bottom of each tank body has filter
Film, after injecting measuring samples, microorganism is enriched with by this filter membrane, and the pipeline of this filter 1100 is three pipelines in parallel,
One end of these three pipelines in parallel is arranged on the top of described filter 1100 and is respectively communicated with respectively this tank body, and these three also
The other end of the pipeline of connection is a filter syringe needle；This filter 1100 has a setting in described enrichment procedure cabin 105
Position, this setting position is that the bottom of each described tank body is respectively placed in waste liquid tank supported hole.
It is arranged over an enrichment procedure mechanical hand 510 at described transporter 400 in described enrichment procedure cabin 105,
The filter syringe needle of described filter 1100 is inserted in measuring samples and changes culture by this enrichment procedure mechanical hand 510
Syringe needle, inserts the culture of culture medium, and this enrichment procedure mechanical hand 510 can make this filter 1100 in this setting position
And move between transporter；This enrichment procedure mechanical hand 510 is provided with one in order to drive near the side in described buffering cabin
Dynamic product to be tested or culture inject the enrichment drive mechanism entering described filter 1100 in described three pipelines in parallel
500；
By filter block head pair after described pre-position is provided with filtration enrichment in described enrichment procedure cabin 105
Each tank body bottom carries out the packaging mechanism 530 closed；
This enrichment procedure mechanical hand 510, enrichment drive mechanism 500 and packaging mechanism 530 are electrically connected this industry control
Machine 800, is controlled by this industrial computer 800.
In a preferred embodiment of the present invention, described enrichment drive mechanism 500 is a peristaltic pump, the pump of this peristaltic pump
Being provided with the first support and the second support at Tou, this first support and this second support are filtered at this enrichment procedure manipulator clamping
Device syringe needle clamps this three pipelines in parallel when inserting in described measuring samples respectively.Wherein, the first support is telescopically
Being fixed on the pump head of this peristaltic pump near described enrichment mechanical hand 510 side, this second support is telescopically and can be around
What the pump head of this peristaltic pump rotated is arranged at the pump head of this peristaltic pump.This second support has a primary importance and one
Two positions, this primary importance is disposed side by side on by this first support, in order to this first support clamp together this this three
Pipeline in parallel, this second position is that the rotation of this second support can put into this to these three pipelines in parallel are smoothed out with the fingers suitable correspondence
Position in the pump head of peristaltic pump.When operation, this first support clamps this three pipes in parallel respectively with this second
Road, then this second support is rotated to the second position, this first support and this second support retraction afterwards, by these three
Pipeline in parallel is put in the pump head of described peristaltic pump.
Described enrichment procedure mechanical hand 510 also comprise one when described filter complete described measuring samples filtration enrichment after,
These three pipelines in parallel can be closed the culture medium heat sealing mechanism of at most two by culture before entering described filter, so
Culture can be selectively implanted in the filter of microorganism enrichment.Can optionally seal in thribble road
One or two or all blow-by.
Three pressure transducers sensing this filter 1100 internal pressure it are additionally provided with in described enrichment procedure cabin 105
900, by the pressure in the pressure regulation enrichment procedure cabin in sensing filter.
This peristaltic pump, this first support, this second support, this culture medium heat sealing mechanism and this three pressure transducer
900 are electrically connected this industrial computer 800.
With this, when operating in described enrichment procedure cabin 105, for following action:
Described filter 1100 is placed on this setting position, the most again by filter pin by enrichment mechanical hand 510
Head inserts to be fond of in sample 1000, by the first support and the second support, the pipeline that three in parallel is put into wriggling
In the pump head of pump, peristaltic pump is started working, and is filtered by filter 1100 by the liquid in measuring samples 1000, will
On the microorganism enrichment filter membrane that may contain, complete to be enriched with work.Packaging mechanism 530 is sealed by filter block head afterwards
Close the bottom of each tank body, to complete encapsulation.Close a pipeline by the choosing of culture medium heat sealing mechanism again, be enriched with afterwards
Filter needle head replacing is culture syringe needle by mechanical hand 510, inserts in culture medium in culture, then by peristaltic pump
Act on and culture is injected two pipelines, be enriched in corresponding two tank body.To sum up, in this enrichment procedure cabin
The enrichment of filter, encapsulation and the injection of culture is completed in 105.
The signal that industrial computer detects according to each sensor perform within control program, control respectively the pressure in each cabin,
The corresponding corresponding actions performing device in the transmission of transporter and each cabin.This control program is those skilled in the art
Common knowledge, be not the present inventor's innovation and creation, therefore do not repeating at this.
With this in a preferred embodiment of the full automatic microorganism enrichment method of the present invention, described filter is three parallel connections
The tank body arranged, can be to two tank bodies filling culture therein.
Preferably in described 5th step, used measuring samples and culture medium pass inner packing back by transporter 400
Remove cabin, reclaim in inner packing removes cabin.
It is preferred that as shown in Figure 1 and Figure 2, in a preferred embodiment of the present invention, in institute in described buffering cabin 106
The pipeline that is arranged over stating transporter 400 cuts mounting mechanism 520, to encapsulate the pipeline of this filter.
This pipeline is cut mounting mechanism 520 and is electrically connected described industrial computer 800.
It is preferred that as shown in Figure 1 and Figure 2, in a preferred embodiment of the present invention, described positive bacteria filling cabin 107
Cabin 107 is filled, to fill cabin at this positive bacteria after this positive bacteria has filled for having the positive bacteria of heating function
Can start in 107 to cultivate.Transporter 400 in this positive bacteria filling cabin 107 is arranged over positive bacteria and adds
Note mechanism 700；It is additionally provided with temperature control equipment in this positive bacteria filling cabin.
This positive bacteria fills mechanism 700 and this heater is electrically connected described industrial computer 800.
It is preferred that this industrial computer 800 uses host computer form, each cabin can have independent control unit.
In sum, the present invention provides a kind of full automatic microorganism detection enrichment system and enrichment method, full-automatic ground
Microorganism in measuring samples is enriched with, it is not necessary to manual operation, time saving and energy saving, and can be prevented effectively from due to artificially
The impact of factor and the false positive that causes or false-negative result, it is achieved the aseptic full automatic working of enrichment process, have
Detect substantial effect accurately, the most error-prone in enrichment process, and can work continuously when enrichment procedure, it is not necessary to stop
After enrichment procedure, sterilization is suspended in enrichment procedure cabin, there is higher implementation result.
Claims (10)
1. a full automatic microorganism detection enrichment system, it is characterised in that it comprises:
One preset operation position, this preset operation position in order to the measuring samples placed, culture medium containing culture,
Collect the special filter with pipeline of bacterium and filter block head；This filter passes through membrane filtration measuring samples, is enriched with to be checked
The microorganism that may contain in sample；
It is disposed adjacently successively behind this preset operation position and carries out the sterilizing cabin of sterilizing, removal filter and filter block head
The outer package of outer package removes the inner packing removing cabin of cabin, removal filter and filter block head inner packing, by measuring samples
Inject filter filter enriched microorganism and encapsulated and filter bottom optionally by culture by filter block head
Inject the enrichment procedure cabin of filter, the pipeline of the filter being enriched with is cut the buffering cabin of dress, carry out positive bacteria and add
The positive bacteria filling cabin of note；Above-mentioned each cabin is connected by nacelle interrupter respectively and closes；
One in order to be respectively fed to phase by the measuring samples on preset operation position, culture medium, filter and filter block head
Answering the transporter in above-mentioned each cabin, this transporter, from the beginning of preset operation position, runs through above-mentioned each cabin；
One industrial computer；This industrial computer electrically connects above-mentioned each cabin, each nacelle interrupter and transporter.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that transmitting dress
Put and be provided with fixing measuring samples, culture medium, filter and the fixing device of filter block head containing culture；With/
Or,
This transporter is divided into measuring samples transporter, culture medium transporter, filter transporter, filter to block
Head transporter；And/or；
This transporter pre-position in each cabin is respectively equipped with alignment sensor, and each alignment sensor is electrically connected respectively
Connect industrial computer；And/or,
This transporter is belt conveyer or roller bearing transporter；By being arranged on each each cabin of driven by motor out of my cabin
In magnetic fluid rotate, to drive belt or roller bearing, or by the motor direct-drive belt being arranged in each cabin or
Roller bearing transmission；And/or,
Respectively this nacelle interrupter is separation valve door.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that described each cabin
It is respectively equipped with outward barometric control unit and the ventilation unit regulating pressure in each cabin；Respectively this barometric control unit and respectively this lead to
Wind apparatus is electrically connected described industrial computer；Respectively include in this barometric control unit and sense the pressure of pressure in corresponding cabin
Sensor, respectively this pressure transducer electrically connects this industrial computer；And/or,
Described sterilizing cabin, enrichment procedure cabin, buffering are respectively equipped with one out of my cabin and go out except the bactericidal unit of antibacterial in corresponding cabin.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that described outsourcing
Dress removes cabin and described inner packing removing cabin and is respectively equipped with removal filter and filter block head above described transporter
The outer package removal mechanism of outer package and inner packing and inner packing removal mechanism；This inner packing removal mechanism and outer package are gone
Except mechanism is electrically connected this industrial computer.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that described with
The filter of pipeline is the tank body that three two ends being arranged in parallel have outlet respectively, and the bottom of each tank body has filter membrane, note
After entering measuring samples, microorganism is enriched with by this filter membrane, and the pipeline of this filter is three pipelines in parallel, these three parallel connections
One end of pipeline be arranged on the top of described filter and be respectively communicated with respectively this tank body, the other end of these three pipelines in parallel
It it is a filter syringe needle；This filter has a setting position in described enrichment procedure cabin, and this setting position is each described tank
The bottom of body is respectively placed in waste liquid tank supported hole；
It is arranged over an enrichment procedure mechanical hand at described transporter, this enrichment procedure machine in described enrichment procedure cabin
The filter syringe needle of described filter is inserted in measuring samples and changes culture syringe needle by tool hands, inserts the cultivation of culture medium
Thing, this enrichment procedure mechanical hand can make this filter move between this setting position and transporter；This enrichment procedure machine
Tool hands is provided with one in order to drive product to be tested or culture to inject described three parallel connections near the side in described buffering cabin
Pipeline in enter the enrichment drive mechanism of described filter；
After described pre-position is provided with filtration enrichment, filter block head is passed through to each tank in described enrichment procedure cabin
Body bottom carries out the packaging mechanism closed；
This enrichment procedure mechanical hand, enrichment drive mechanism and packaging mechanism are electrically connected this industrial computer.
Full automatic microorganism the most according to claim 5 detection enrichment system, it is characterised in that described enrichment
Drive mechanism is a peristaltic pump, is provided with the first support and the second support at the pump head of this peristaltic pump, this first support and should
Second support clamps these three respectively when this enrichment procedure manipulator clamping filter syringe needle inserts in described measuring samples
Pipeline in parallel, the first support is telescopically fixed on the pump head of this peristaltic pump near described enrichment mechanical hand side, should
Second support telescopically and can be arranged at the pump head of this peristaltic pump around what the pump head of this peristaltic pump rotated, and this is second years old
Support has a primary importance and a second position, and this primary importance is disposed side by side on by this first support, in order to being somebody's turn to do
First support clamps this three pipelines in parallel together, and this second position is that this second support rotates to by these three parallel connections
Pipeline smooth out with the fingers the position that can put in the pump head of this peristaltic pump along correspondence；When operation, this first support and this second
Clamp this three pipelines in parallel respectively, then this second support is rotated to the second position, afterwards this first support and should
These three pipelines in parallel are put in the pump head of described peristaltic pump by the second support retraction；
Described enrichment procedure mechanical hand also comprise one when described filter complete described measuring samples filtration enrichment after, cultivate
Thing enters the culture medium heat sealing mechanism that these three pipelines in parallel can be closed before described filter at most two, so can be by
Culture has optionally injected in the filter of microorganism enrichment；
Three pressure transducers sensing this filter internal pressure it are additionally provided with in described enrichment procedure cabin；
This peristaltic pump, this first support, this second support, this culture medium heat sealing mechanism and this three pressure transducer divide
Do not electrically connect this industrial computer.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that described buffering
In cabin, the pipeline that is arranged at described transporter cuts mounting mechanism；
This pipeline is cut mounting mechanism and is electrically connected described industrial computer.
Full automatic microorganism the most according to claim 1 detection enrichment system, it is characterised in that the described positive
Bacterium filling cabin is the positive bacteria filling cabin with heating function, and the transporter in this positive bacteria filling cabin is arranged over
Positive bacteria fills mechanism；It is additionally provided with temperature control equipment in this positive bacteria filling cabin；
This positive bacteria filling mechanism and this heater are electrically connected described industrial computer；And/or,
This industrial computer uses host computer form, and each cabin can have independent control unit.
9. according to the full automatic microorganism enrichment method described in any of the above-described claim, it is characterised in that it comprises
Following steps:
The first step: by the filter with pipeline special to measuring samples, culture medium containing culture, collection bacterium and filter
Block head is placed on the described transporter of preset operation position；
Second step: open the nacelle interrupter between preset operation position and sterilizing cabin, by measuring samples, containing training
Supporting the culture medium of thing, collect in the special filter with pipeline of bacterium and the incoming described sterilizing cabin of filter block head, closedown is gone out
The nacelle interrupter of both sides, bacterium cabin, carries out sterilizing, and after sterilizing completes, ventilation balances pressure in sterilizing cabin；
3rd step: open sterilizing cabin and the nacelle interrupter between cabin is removed in outer package, by measuring samples, containing training
Support the culture medium of thing, collect in the special filter with pipeline of bacterium and filter block head incoming described outer package removal cabin,
Closing outer package and remove the nacelle interrupter of both sides, cabin, the outer package carrying out filter and filter block head is removed；
4th step: open outer package and remove cabin and inner packing and remove the nacelle interrupter between cabin, by measuring samples,
The filter with pipeline and the incoming described inner packing of filter block head that culture medium containing culture, collection bacterium are special are removed
In cabin, closing inner packing and remove the nacelle interrupter of both sides, cabin, the inner packing carrying out filter and filter block head is removed；
5th step: open inner packing and remove the nacelle interrupter between cabin and enrichment procedure cabin, by measuring samples, contain
There is the culture medium of culture, collect the special filter with pipeline of bacterium and the incoming described enrichment procedure cabin of filter block head
In, the microbe filter in measuring samples is enriched in filter, then by filter block head, filter bottom is encapsulated,
The most optionally culture is injected filter, be placed on being enriched with the good filter of post package on transporter, it is thus achieved that phase
After answering the filter that the enrichment post package of quantity is good, close the nacelle interrupter of both sides, enrichment procedure cabin；
6th step: open the nacelle interrupter between enrichment procedure cabin and buffering cabin, the filter good by being enriched with post package
In incoming described buffering cabin, close the nacelle interrupter of buffering both sides, cabin, the pipeline of the filter good to enrichment post package
Carry out cutting dress；
7th step, opens the nacelle interrupter between buffering cabin and positive bacteria filling cabin, enrichment post package is completed well
Cut the filter incoming positive bacteria filling cabin of dress, close the nacelle interrupter of positive bacteria filling both sides, cabin, after being enriched with
The packaged filter filling positive bacteria completing to cut dress, completes the detection enrichment of the microorganism of test sample.
Full automatic microorganism enrichment method the most according to claim 9, it is characterised in that preset operation position,
Sterilizing cabin, outer package remove cabin, inner packing removing nacelle interrupter between cabin, enrichment procedure cabin is opened each time
Time, the pressure in previous cabin is less than the pressure in later cabin, it is ensured that antibacterial is not brought into enrichment procedure cabin；Enrichment procedure
When nacelle interrupter between cabin, buffering cabin and positive bacteria filling cabin is opened each time, the pressure in previous cabin wants height
Pressure in later cabin, it is ensured that positive bacteria is not the most inversely delivered in enrichment procedure cabin；And/or,
In described 7th step, after closing the nacelle interrupter of positive bacteria filling both sides, cabin, also comprise a step: to institute
State buffering cabin and carry out sterilizing；And/or,
Described filter is the tank body that three two ends being arranged in parallel have outlet respectively, optionally to the most at most two
Tank body filling culture；And/or,
In described 5th step, used measuring samples and culture medium are passed inner packing back by transporter and are removed cabin,
Inner packing is removed in cabin and is reclaimed.
Priority Applications (8)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310751864.2A CN104745464B (en) | 2013-12-31 | 2013-12-31 | Full automatic microorganism detection enrichment system and enrichment method thereof |
| EP14877171.0A EP3091068B1 (en) | 2013-12-31 | 2014-12-31 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| AU2014375463A AU2014375463B2 (en) | 2013-12-31 | 2014-12-31 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| KR1020167020524A KR101807984B1 (en) | 2013-12-31 | 2014-12-31 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| JP2016561066A JP2017502696A (en) | 2013-12-31 | 2014-12-31 | Fully automatic microbial detection integration system and its integration method |
| CA2935299A CA2935299C (en) | 2013-12-31 | 2014-12-31 | Full-automatic microorganism detecting enrichment system and enrichment method thereof |
| PCT/CN2014/095848 WO2015101326A1 (en) | 2013-12-31 | 2014-12-31 | Full-automatic microbial detection and enrichment system and enrichment method thereof |
| US15/106,930 US20170029760A1 (en) | 2013-12-31 | 2014-12-31 | Full-automatic microorganism detecting enrichment system and enrichment method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310751864.2A CN104745464B (en) | 2013-12-31 | 2013-12-31 | Full automatic microorganism detection enrichment system and enrichment method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104745464A CN104745464A (en) | 2015-07-01 |
| CN104745464B true CN104745464B (en) | 2016-09-14 |
Family
ID=53493267
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310751864.2A Active CN104745464B (en) | 2013-12-31 | 2013-12-31 | Full automatic microorganism detection enrichment system and enrichment method thereof |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20170029760A1 (en) |
| EP (1) | EP3091068B1 (en) |
| JP (1) | JP2017502696A (en) |
| KR (1) | KR101807984B1 (en) |
| CN (1) | CN104745464B (en) |
| AU (1) | AU2014375463B2 (en) |
| CA (1) | CA2935299C (en) |
| WO (1) | WO2015101326A1 (en) |
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| US10897922B2 (en) | 2016-04-05 | 2021-01-26 | Nch Corporation | Composition and method for germinative compounds in probiotic food and beverage products for human consumption |
| CN106226151B (en) * | 2016-07-22 | 2019-03-08 | 牛刚 | Sterile cabin automatic medicine sterility test enrichment system and its operating method |
| CN106010955B (en) * | 2016-07-22 | 2018-12-11 | 牛刚 | Sterile cabin pharmaceutical sterile test is enriched with Operation Van |
| CN107505439A (en) * | 2017-07-27 | 2017-12-22 | 马鹏飞 | A kind of medicine sterile detector based on robot motion's technology |
| US11155780B2 (en) | 2017-10-04 | 2021-10-26 | NCH Life Sciences LLC | Metastable state mixing |
| CN108426941A (en) * | 2017-12-31 | 2018-08-21 | 宁波华仪宁创智能科技有限公司 | A kind of safety check instrument and method |
| CN107988069A (en) * | 2018-01-30 | 2018-05-04 | 杨军 | A kind of medical Micro biological Tests quarantine culture apparatus |
| CN108611264A (en) * | 2018-05-10 | 2018-10-02 | 朱丹 | A kind of system and method that multiple batches of biological products party-line basis is standby |
| CN109266521A (en) * | 2018-09-18 | 2019-01-25 | 江门出入境检验检疫局检验检疫技术中心 | Pneumatic type multichannel microbiological culture media prepares packing instrument automatically |
| CN109459410A (en) * | 2018-11-21 | 2019-03-12 | 郑州博莱特生物科技有限公司 | The automatic rapid detection system of food microorganisms |
| CN109795857A (en) * | 2019-02-19 | 2019-05-24 | 浙江大学 | Isolator collection bacterium operation transmission system |
| CN110734845B (en) * | 2019-11-22 | 2023-11-24 | 中国科学院海洋研究所 | In-situ detection device and method for primary productivity of microorganisms in deep sea extreme environment based on ROV |
| CN112226348B (en) * | 2020-10-26 | 2023-06-16 | 达尔文实验机器人成都有限公司 | Bacteria collecting method of microbial detection robot system using biological medicine injection |
| CN113138109B (en) * | 2021-04-23 | 2022-07-01 | 贵州省产品质量检验检测院 | Rotary repeated type device for extracting and enriching trace components in sample |
| CN113549534A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria-collecting operation system's control infusion and bacteria-collecting device |
| CN113549540A (en) * | 2021-06-29 | 2021-10-26 | 杭州电子科技大学 | Intelligent bacteria collecting system |
| CN113897274B (en) * | 2021-10-21 | 2022-05-06 | 九江学院 | Microorganism enrichment culture apparatus |
| CN114350509B (en) * | 2022-03-17 | 2022-11-04 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment biological enrichment and spraying type solid separation culture device |
| CN114350507A (en) * | 2022-03-17 | 2022-04-15 | 广东工业大学 | Single colony separation device and separation method for deep sea in-situ environment |
| CN114350508B (en) * | 2022-03-17 | 2022-07-12 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism enrichment culture and gravity type separation device |
| CN114456919B (en) * | 2022-03-17 | 2022-09-16 | 南方海洋科学与工程广东省实验室(广州) | High-pressure environment marine microorganism solid separation culture device and culture method |
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Also Published As
| Publication number | Publication date |
|---|---|
| CA2935299A1 (en) | 2015-07-09 |
| EP3091068A4 (en) | 2017-10-18 |
| CA2935299C (en) | 2019-01-22 |
| JP2017502696A (en) | 2017-01-26 |
| KR20160104029A (en) | 2016-09-02 |
| AU2014375463A1 (en) | 2016-07-07 |
| EP3091068B1 (en) | 2019-01-09 |
| US20170029760A1 (en) | 2017-02-02 |
| WO2015101326A1 (en) | 2015-07-09 |
| AU2014375463B2 (en) | 2017-05-25 |
| KR101807984B1 (en) | 2017-12-11 |
| CN104745464A (en) | 2015-07-01 |
| EP3091068A1 (en) | 2016-11-09 |
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