CN116273251B - Sterile operation device for stem cell preparation and application method thereof - Google Patents
Sterile operation device for stem cell preparation and application method thereof Download PDFInfo
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- CN116273251B CN116273251B CN202310120838.3A CN202310120838A CN116273251B CN 116273251 B CN116273251 B CN 116273251B CN 202310120838 A CN202310120838 A CN 202310120838A CN 116273251 B CN116273251 B CN 116273251B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 36
- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000005192 partition Methods 0.000 claims abstract description 52
- 238000009423 ventilation Methods 0.000 claims abstract description 41
- 230000007246 mechanism Effects 0.000 claims abstract description 35
- 230000005540 biological transmission Effects 0.000 claims abstract description 10
- 238000000638 solvent extraction Methods 0.000 claims abstract description 3
- 230000001360 synchronised effect Effects 0.000 claims description 14
- 229910000831 Steel Inorganic materials 0.000 claims description 9
- 230000008569 process Effects 0.000 claims description 9
- 239000010959 steel Substances 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 8
- 239000000284 extract Substances 0.000 claims description 8
- 239000011521 glass Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 238000002955 isolation Methods 0.000 claims description 4
- 230000002070 germicidal effect Effects 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 6
- 230000000694 effects Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 2
- 210000004504 adult stem cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002894 multi-fate stem cell Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/02—Laboratory benches or tables; Fittings therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L1/00—Enclosures; Chambers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P40/00—Technologies relating to the processing of minerals
- Y02P40/50—Glass production, e.g. reusing waste heat during processing or shaping
- Y02P40/57—Improving the yield, e-g- reduction of reject rates
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- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a sterile operation device for stem cell preparation and a use method thereof, and belongs to the technical field of biomedical equipment. The sterile operating device for stem cell preparation comprises a support column and a sterile base arranged at the top of the support column, wherein the upper end of the sterile base is connected with an operating shell, the top of the operating shell is connected with a circulating bellows, an operating cavity and a conveying channel which are mutually communicated are arranged in the operating shell, a pushing mechanism is arranged in the operating cavity, a partition component for partitioning the operating cavity and the conveying channel is arranged on the inner wall of the conveying channel, two groups of sliding doors are slidably connected at the front end of the operating shell, a ventilation mechanism for replacing air in the conveying channel is arranged between the sliding doors and the partition component, and the ventilation mechanism is connected with a transmission component for driving the sliding doors to displace; the invention can rapidly take and put the glassware to be tested, has simple and convenient operation, effectively prevents bacteria from entering the operation space, and improves the working efficiency of aseptic operation.
Description
Technical Field
The invention relates to the technical field of biomedical equipment, in particular to a sterile operation device for stem cell preparation and a use method thereof.
Background
The stem cells are multipotent cells with self-replication capability, can be differentiated into a plurality of functional cells under certain conditions, are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, are immature cells, have the potential functions of regenerating various tissues, organs and human bodies, are called universal cells in the medical field, are manufactured by using a sterile operation device in the stem cell preparation process, and are necessary equipment in the biological medicine research field, so that a sterile clean space can be provided for experimental operation of scientific researchers.
Among the prior art, patent application number CN202022813376.8 discloses a sterile operation device that stem cell preparation was used, removes the sliding door through manual, then controls electric putter and releases the operation shell with the movable frame to the convenient glassware of getting of having played, guarantee the effect that the hand is not scalded. However, in the specific operation process, the picking and placing operation is still complicated, and the operation work efficiency of stem cell preparation cannot be improved; and when placing glassware, open with the in-process of closing the sliding door, can have the inside phenomenon of bacterium entering operation panel, though accessible circulation bellows is changed the air in the operation shell, but because the operation shell inner space is great for the gas change speed is slower, further influences the work efficiency of stem cell preparation.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a sterile operation device for stem cell preparation and a use method thereof.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the utility model provides an aseptic operating device for stem cell preparation, includes the support column and sets up the aseptic base at the support column top, aseptic base's upper end is connected with the operation shell, the top of operation shell is connected with circulation bellows, be provided with operation chamber and the conveying passageway of mutual intercommunication in the operation shell, be provided with pushing mechanism in the operation chamber, the conveying passageway inner wall is provided with the partition assembly that is used for cutting off operation chamber and conveying passageway, the front end sliding connection of operation shell has two sets of sliding doors, be provided with the breather mechanism that is used for changing conveying passageway inside air between sliding door and the partition assembly, just breather mechanism is connected with the drive assembly that is used for driving the sliding door displacement.
Preferably, the partition assembly comprises two partition plates which are movably abutted against each other, the partition plates are rotatably connected in the conveying channel through a rotating shaft, torsion springs are sleeved on the rotating shaft, sealing sleeves are sleeved on the outer sides of the partition plates, and the sealing sleeves are movably abutted against the inner wall of the conveying channel.
Preferably, the pushing mechanism comprises an electric push rod fixedly arranged on the inner wall of the operation cavity, an arc-shaped groove matched with the electric push rod is formed in the partition plate, one end, far away from the inner wall of the operation cavity, of the electric push rod is connected with a mounting plate, a placing frame is arranged on the mounting plate, the placing frame is movably abutted against the partition plate, and a steel wire mesh used for placing glassware is arranged in the placing frame.
Preferably, the ventilation mechanism comprises a ventilation assembly and a driving assembly for driving the ventilation assembly to act, the ventilation assembly comprises ventilation cavities arranged on two sides of the operation shell, the ventilation cavities are respectively provided with a first air cavity and a second air cavity, each ventilation cavity is internally provided with a piston plate in a sliding manner, a first elastic element is arranged between each piston plate and the inner wall of the corresponding ventilation cavity, each first air cavity is provided with a first air inlet valve and a first air outlet valve, each first air cavity is communicated with the operation cavity through a first air inlet valve, each first air cavity is connected with the conveying channel through a first air outlet valve, each second air cavity is provided with a second air inlet valve and a second air outlet valve, each second air cavity is connected with the conveying channel through a second air inlet valve, and each second air cavity is communicated with the outside atmosphere through a second air outlet valve.
Preferably, the inner wall of the conveying channel is provided with an ultraviolet sterilizing lamp, and the inner wall of the conveying channel is provided with an arc-shaped guide surface.
Preferably, the drive assembly includes the dwang that rotates the setting in the operation shell, be provided with driven gear on the dwang, place the bottom of frame and be provided with the rack board of being connected with driven gear meshing, the dwang outside still overlaps and is equipped with the stay cord, the one end that the dwang was kept away from to the stay cord passes the ventilation chamber and links to each other with the piston board.
Preferably, the transmission assembly comprises a screw rod which is rotatably arranged in an operation shell, synchronous wheels are arranged on the screw rod and the rotating rod, a synchronous belt is arranged between the synchronous wheels, a sleeve is connected with the outer side of the screw rod in a threaded manner, the sleeve is fixedly connected with a sliding door, and a sliding groove for sliding the sleeve is formed in the operation shell.
Preferably, the movable groove communicated with the ventilation cavity is formed in the operation shell, the limiting block is connected in the movable groove in a sliding mode, a limiting hole matched with the limiting block is formed in the piston plate, a containing groove matched with the limiting block is formed in the sliding door, the limiting block comprises a first block body, a second block body and a third elastic element connected with the first block body and the second block body, a sliding block is connected to the first block body, a sliding groove matched with the sliding block is formed in the inner wall of the movable groove, and a second elastic element is arranged between the inner wall of the sliding groove and the sliding block.
Preferably, the operation shell is embedded with a perspective window, and the perspective window is provided with a bacteria isolation operation glove.
The invention also discloses a using method of the sterile operation device for stem cell preparation, which comprises the following steps:
s1: when the device is used, the circulating bellows works to create a sterile environment in the operation shell, the electric push rod is controlled to start, the electric push rod drives the placing frame to move into the conveying channel from the operation cavity through the mounting plate, in the process, the placing frame offsets the partition plates and acts force, the partition plates are stressed to deflect by taking the rotating shaft as the center, after the electric push rod drives the placing frame to pass over the partition plates, the partition plates reset and rotate under the action of the torsion springs, the two partition plates offset again, the arc-shaped grooves on the two partition plates adapt to the telescopic parts of the electric push rod, and at the moment, the partition plates separate the operation cavity from the conveying channel;
s2: along with the continuous movement of the placing frame in the conveying channel driven by the electric push rod, a rack plate on the placing frame is meshed with a driven gear on the rotating rod, the driven gear drives the rotating rod to rotate and wind up a pull rope, the pull rope drives a piston plate to slide in a ventilation cavity during wind up, a first elastic element deforms, a piston plate in a first air cavity extracts clean air in an operation cavity and a circulating bellows through a first air inlet valve, and a piston plate in a second air cavity discharges air in the cavity through a second air outlet valve;
s3: when the rack plate is meshed with the driven gear, the piston plate moves to the position of the limiting block and can be limited by the limiting block, and at the moment, the sliding doors at two sides are relatively far away, a conveying channel is opened, so that an operator can conveniently take and put glassware, and hands are prevented from being scalded;
s4: after the glass ware is placed on the steel wire gauze of the placement frame, the electric push rod is controlled to drive the placement frame to reset, so that the rack plate is meshed with the driven gear again, the rotary rod and the screw rod rotate reversely, the pull rope is released by the rotary rod, the screw rod drives the sliding door to reset to block the conveying channel again through the sleeve, after the sliding door is reset, the accommodating groove of the sliding door is opposite to the limiting block, the limiting block is not extruded by the sliding door any more, the limiting block resets under the action of the second elastic element, the limiting block moves out of the limiting hole, the piston plate in the air exchanging cavity resets under the pulling of the first elastic element, the second air cavity extracts air mixed with the outside in the conveying channel through the second air inlet valve, the first air cavity discharges the extracted clean air into the conveying channel through the first air outlet valve, air conversion in the conveying channel is completed in the process of conveying the glass ware, and then the placement frame is propped against the partition plate again, so that the placing frame is overturned along with the electric push rod again into the operation cavity;
s5: the staff observe and operate the stem cells in the glassware through the bacteria isolation operation glove at the perspective window.
Compared with the prior art, the invention provides a sterile operation device for stem cell preparation and a use method thereof, and the sterile operation device has the following beneficial effects:
1. according to the sterile operation device for stem cell preparation and the application method thereof, when the pushing mechanism pushes the test device, the pushing mechanism drives the air exchanging mechanism to act, the air exchanging mechanism drives the transmission assembly to act, the transmission assembly drives the sliding door to move away, only the electric push rod is required to be driven to act, the sliding door is not required to be manually moved away, the operation steps are simplified, and the work efficiency of stem cell preparation is improved conveniently.
2. According to the sterile operation device for stem cell preparation and the application method thereof, when a pushing mechanism pushes a test device, the ventilation mechanism works, automatic replacement of air intersecting with external air when the sliding door is opened and closed is completed, all replacement of air in the operation shell is not needed through the circulating bellows, the replacement speed of gas is improved, and further the preparation work efficiency of stem cells is improved.
3. According to the sterile operation device for stem cell preparation and the use method thereof, the piston plate is limited by the limiting block, the electric push rod drives the placement frame to move back, the limitation on the piston plate can be relieved only after the sliding door is reset to block the conveying channel again, the first air cavity and the second air cavity are enabled to replace polluted air between the partition plate and the sliding door, the situation that the sliding door does not block the conveying channel yet is avoided, the conveying channel is still communicated with outside air, the ventilation mechanism starts to act, and the air replacement effect and the replacement efficiency in the conveying channel are affected.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic cross-sectional view of the operation housing of the present invention;
FIG. 3 is a partially enlarged schematic illustration of the structure of portion A of FIG. 2 in accordance with the present invention;
FIG. 4 is a schematic diagram showing a cross-sectional structure of a handling case according to the present invention;
FIG. 5 is a partially enlarged schematic illustration of the structure of portion B of FIG. 4 in accordance with the present invention;
FIG. 6 is a schematic view of a cross-sectional structure of a handling shell according to the present invention;
FIG. 7 is a schematic diagram of a pushing mechanism according to the present invention;
FIG. 8 is a schematic view of the external structure of the rotating lever of the present invention;
FIG. 9 is a schematic view of a stopper according to the present invention;
FIG. 10 is a schematic view of the structure of the separator of the present invention;
fig. 11 is a schematic structural view of a perspective window according to the present invention.
In the figure: 1. a sterile base; 101. a support column; 2. an operation case; 201. an operating chamber; 202. a conveying channel; 3. a circulating bellows; 4. a sliding door; 401. a receiving groove; 5. a partition plate; 501. sealing sleeve; 502. an arc-shaped groove; 6. an electric push rod; 601. a mounting plate; 602. placing a frame; 6021. rack plate; 603. a steel wire mesh; 7. an air exchanging cavity; 701. a first air chamber; 702. a second air chamber; 8. a piston plate; 801. a first elastic element; 802. a limiting hole; 9. a rotating lever; 901. a driven gear; 902. a pull rope; 10. a synchronizing wheel; 11. a screw rod; 111. a sleeve; 12. a sliding groove; 13. a movable groove; 131. a chute; 1311. a second elastic element; 14. a limiting block; 141. a first block; 1411. a slide block; 142. a second block; 143. a third elastic element; 15. an ultraviolet germicidal lamp; 16. a perspective window; 161. the glove is operated by bacteria isolation.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.
In the description of the present invention, it should be understood that the terms "upper," "lower," "front," "rear," "left," "right," "top," "bottom," "inner," "outer," and the like indicate or are based on the orientation or positional relationship shown in the drawings, merely to facilitate description of the present invention and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Example 1:
referring to fig. 1, fig. 2, fig. 3, fig. 4, fig. 5, fig. 6 and fig. 7, a sterile operation device for stem cell preparation comprises a support column 101 and a sterile base 1 arranged at the top of the support column 101, wherein the upper end of the sterile base 1 is connected with an operation shell 2, the top of the operation shell 2 is connected with a circulating bellows 3, an operation cavity 201 and a conveying channel 202 which are mutually communicated are arranged in the operation shell 2, a pushing mechanism is arranged in the operation cavity 201, a partition component for partitioning the operation cavity 201 and the conveying channel 202 is arranged on the inner wall of the conveying channel 202, two groups of sliding doors 4 are slidably connected at the front end of the operation shell 2, a ventilation mechanism for replacing air in the conveying channel 202 is arranged between the sliding doors 4 and the partition component, and the ventilation mechanism is connected with a transmission component for driving the sliding doors 4 to displace.
Specifically, when the device is used, the circulating bellows 3 works to create a sterile environment in the operation shell 2, the pushing mechanism pushes a test device such as glassware into the conveying channel 202 from the operation cavity 201, when the pushing mechanism pushes the test device, the pushing mechanism drives the air exchanging mechanism to act, the air exchanging mechanism exchanges external air entering the conveying channel 202, the air exchanging mechanism drives the transmission assembly to act, the transmission assembly drives the sliding door 4 to move away, and only the pushing mechanism is controlled to act, so that the sliding door 4 does not need to be manually moved away.
Example 2:
referring to fig. 2, 4 and 10, based on embodiment 1, a sterile operation device for stem cell preparation is further provided, wherein the partition assembly comprises two movable partition plates 5, the partition plates 5 are rotatably connected in the conveying channel 202 through a rotating shaft, torsion springs are sleeved on the rotating shaft, sealing sleeves 501 are sleeved on the outer sides of the partition plates 5, and the sealing sleeves 501 are movably abutted against the inner wall of the conveying channel 202.
Specifically, through setting up baffle 5 and can cut off operating chamber 201 and conveying passageway 202, baffle 5 can elastically rotate and set up in conveying passageway 202, be convenient for cooperate pushing action of pushing mechanism, and the cover is equipped with seal cover 501 in the outside cover of baffle 5, can improve the sealed effect that baffle 5 cut off operating chamber 201 and conveying passageway 202.
Example 3:
referring to fig. 2, fig. 4 and fig. 7, a sterile operation device for stem cell preparation, on the basis of embodiment 2, further, a pushing mechanism includes an electric push rod 6 fixedly arranged on the inner wall of an operation cavity 201, an arc-shaped groove 502 matched with the electric push rod 6 is formed on a partition board 5, one end, far away from the inner wall of the operation cavity 201, of the electric push rod 6 is connected with a mounting plate 601, a placement frame 602 is arranged on the mounting plate 601, the placement frame 602 is movably abutted against the partition board 5, and a steel wire mesh 603 for placing glassware is arranged in the placement frame 602.
Specifically, by controlling the starting of the electric push rod 6, the electric push rod 6 drives the placing frame 602 to move from the operation cavity 201 to the conveying channel 202 through the mounting plate 601, and the glassware for test is placed on the steel wire mesh 603, so that clean air flow passes through the steel wire mesh 603 to ensure the sterile environment of the glassware.
Example 4:
referring to fig. 2, 3, 4, 5, 6 and 8, based on embodiment 3, a sterile operation device for stem cell preparation is further provided, wherein the ventilation mechanism comprises a ventilation assembly and a driving assembly for driving the ventilation assembly to act, the ventilation assembly comprises ventilation cavities 7 arranged on two sides of the operation shell 2, two ventilation cavities 7 are provided with a first air cavity 701 and a second air cavity 702, a piston plate 8 is slidably connected in each ventilation cavity 7, a first elastic element 801 is arranged between the piston plate 8 and the inner wall of the ventilation cavity 7, a first air inlet valve and a first air outlet valve are arranged on the first air cavity 701, the first air cavity 701 is mutually communicated with the operation cavity 201 through the first air inlet valve, the first air cavity 701 is connected with the conveying channel 202 through the first air outlet valve, a second air inlet valve and the second air outlet valve are arranged on the second air cavity 702 and are mutually communicated with the outside atmosphere through the second air outlet valve, and the second air cavity 702 is connected with the conveying channel 202 through the second air inlet valve.
Further, the driving assembly comprises a rotating rod 9 rotatably arranged in the operating shell 2, a driven gear 901 is arranged on the rotating rod 9, a rack plate 6021 meshed with the driven gear 901 is arranged at the bottom of the placing frame 602, a pull rope 902 is further sleeved on the outer side of the rotating rod 9, and one end, far away from the rotating rod 9, of the pull rope 902 penetrates through the air exchanging cavity 7 and is connected with the piston plate 8.
Specifically, as the electric push rod 6 drives the placement frame 602 to move continuously in the conveying channel 202, the rack plate 6021 on the placement frame 602 is meshed with the driven gear 901 on the rotating rod 9, the driven gear 901 drives the rotating rod 9 to rotate and wind the pull rope 902, the pull rope 902 drives the piston plate 8 to slide in the ventilation cavity 7 during winding, the first elastic element 801 deforms, the piston plate 8 in the first air cavity 701 extracts clean air in the operation cavity 201 and the circulating bellows 3 through the first air inlet valve, and the piston plate 8 in the second air cavity 702 discharges air in the cavity through the second air outlet valve; when the electric push rod 6 drives the placement frame 602 to move back, the rotary rod 9 rotates reversely, the rotary rod 9 releases the pull rope 902, the piston plate 8 in the ventilation cavity 7 is reset under the pull of the first elastic element 801, the second air cavity 702 extracts air fused with the outside in the conveying channel 202 through the second air inlet valve, the first air cavity 701 discharges the extracted clean air into the conveying channel 202 through the first air outlet valve, air conversion in the conveying channel 202 is completed in the process of conveying glassware, and the exhaust position of the first air cavity 701 and the air suction position of the second air cavity 702 are arranged oppositely, so that one side sucks air, one side exhausts air, and air in the conveying channel 202, which is contacted with the outside, is convenient to expel effectively.
Example 5:
referring to fig. 2, 4 and 6, an aseptic technique for stem cell preparation is further provided, based on embodiment 4, in which the inner wall of the delivery channel 202 is provided with the ultraviolet germicidal lamp 15, and the inner wall of the delivery channel 202 is provided with an arc-shaped flow guiding surface.
Specifically, through setting the inner wall of the conveying channel 202 as the arc guide surface, when being convenient for change air, the air gets into the second air cavity 702 fast and accurately, improves the ventilation effect, and is provided with the sterilamp 15 in the conveying channel 202, can eliminate the bacterium of glassware outside adhesion.
Example 6:
referring to fig. 2, 3, 4, 5 and 6, a sterile operation device for stem cell preparation is further provided, based on embodiment 4, that the transmission assembly includes a screw rod 11 rotatably disposed in the operation housing 2, synchronizing wheels 10 are disposed on the screw rod 11 and the rotation rod 9, a synchronous belt is disposed between the two synchronizing wheels 10, a sleeve 111 is screwed on the outer side of the screw rod 11, the sleeve 111 is fixedly connected with the sliding door 4, and a sliding groove 12 for sliding the sleeve 111 is formed in the operation housing 2.
Specifically, after the rotating rod 9 rotates, the screw rod 11 rotates along with the rotating rod 9 through the synchronizing wheel 10 and the synchronous belt, the sleeve 111 sleeved on the outer side threads can drive the sliding door 4 to move when the screw rod 11 rotates, the sliding doors 4 on the two sides are relatively far away, the conveying channel 202 is opened, an operator can conveniently take and put glassware, and hands are guaranteed not to be scalded.
Example 7:
referring to fig. 2, 3, 4, 5 and 9, based on embodiment 6, further, a movable slot 13 communicating with the ventilation cavity 7 is provided on the operation housing 2, a stopper 14 is slidably connected in the movable slot 13, a stopper hole 802 matching with the stopper 14 is provided on the piston plate 8, a containing slot 401 matching with the stopper 14 is provided on the sliding door 4, the stopper 14 includes a first block 141, a second block 142 and a third elastic element 143 connecting the first block 141 and the second block 142, a sliding block 1411 is connected on the first block 141, a sliding slot 131 matching with the sliding block 1411 is provided on the inner wall of the movable slot 13, and a second elastic element 1311 is provided between the inner wall of the sliding slot 131 and the sliding block 1411.
Specifically, at the beginning of rotation of the rotating rod 9, one end of the limiting block 14 is placed in the accommodating groove 401 of the sliding door 4, after the rotating rod 9 rotates, the screw rod 11 rotates along with the rotating rod 9 through the synchronous wheel 10 and the synchronous belt, the sleeve 111 sleeved on the outer thread of the screw rod 11 drives the sliding door 4 to displace when the screw rod 11 rotates, the sliding door 4 extrudes the limiting block 14 to enable the limiting block 14 to move into the ventilation cavity 7, when the rack plate 6021 and the driven gear 901 are about to be meshed, the piston plate 8 abuts against the second block 142, the third elastic element 143 is extruded, the second block 142 moves into the movable groove 13, and when the limiting hole 802 on the piston plate 8 is opposite to the second block 142, the limiting block 14 is limited; when the electric push rod 6 drives the placement frame 602 to reset, so that the rack plate 6021 is meshed with the driven gear 901 again, the rotary rod 9 and the screw rod 11 reversely rotate, the rotary rod 9 releases the pull rope 902, the screw rod 11 drives the sliding door 4 to reset through the sleeve 111 to re-plug the conveying channel 202, after the sliding door 4 is reset, the accommodating groove 401 of the sliding door 4 is opposite to the limiting block 14, at the moment, the limiting block 14 is not extruded by the sliding door 4 any more, the limiting block 14 is reset under the action of the second elastic element 1311, the limiting block 14 moves out of the limiting hole 802, the piston plate 8 in the ventilation cavity 7 is reset under the pulling of the first elastic element 801, the second air cavity 702 extracts air mixed with the outside in the conveying channel 202 through the second air inlet valve, the first air cavity 701 discharges the extracted clean air into the conveying channel 202 through the first air outlet valve, and air conversion in the conveying channel 202 is completed in the process of conveying glassware; the arrangement of the limiting block 14 can avoid that when the sliding door 4 does not block the conveying channel 202, the conveying channel 202 is still communicated with the outside air, so that the ventilation mechanism starts to act, and the gas replacement effect and the replacement efficiency in the conveying channel 202 are affected.
Example 8:
referring to fig. 1 and 11, an aseptic manipulation apparatus for stem cell preparation is further provided, based on embodiment 1, with a transparent window 16 embedded in the manipulation case 2, and a bacteria-isolating manipulation glove 161 provided on the transparent window 16.
Specifically, the viewing window 16 and the bacteria-isolating operation glove 161 facilitate the observation operation of the glassware in the operation chamber 201, and the convenience of the operator is improved.
The invention also discloses a using method of the sterile operation device for stem cell preparation, which comprises the following steps:
s1: when the device is used, the circulating bellows 3 works to create a sterile environment in the operation shell 2, the electric push rod 6 is controlled to start, the electric push rod 6 drives the placement frame 602 to move from the operation cavity 201 to the conveying channel 202 through the mounting plate 601, in the process, the placement frame 602 offsets the partition plate 5 and acts force, the partition plate 5 is stressed to deflect by taking the rotating shaft as the center, after the electric push rod 6 drives the placement frame 602 to pass over the partition plate 5, the partition plate 5 resets and rotates under the action of the torsion spring, the two partition plates 5 offset again, the arc-shaped grooves 502 on the two partition plates 5 adapt to the telescopic parts of the electric push rod 6, and at the moment, the partition plate 5 cuts off the operation cavity 201 from the conveying channel 202;
s2: as the electric push rod 6 drives the placing frame 602 to move continuously in the conveying channel 202, the rack plate 6021 on the placing frame 602 is meshed with the driven gear 901 on the rotating rod 9, the driven gear 901 drives the rotating rod 9 to rotate and rolls up the pull rope 902, the piston plate 8 is driven to slide in the ventilation cavity 7 when the pull rope 902 is rolled up, the first elastic element 801 is deformed, the piston plate 8 in the first air cavity 701 extracts clean air in the operation cavity 201 and the circulating bellows 3 through the first air inlet valve, and the piston plate 8 in the second air cavity 702 discharges air in the cavity through the second air outlet valve;
s3: when the rotating rod 9 rotates initially, one end of the limiting block 14 is arranged in the accommodating groove 401 of the sliding door 4, after the rotating rod 9 rotates, the screw rod 11 rotates along with the rotating rod 9 through the synchronous wheel 10 and the synchronous belt, the sleeve 111 sleeved on the outer side threads drives the sliding door 4 to displace when the screw rod 11 rotates, the sliding door 4 extrudes the limiting block 14, so that the limiting block 14 moves into the ventilation cavity 7, when the rack plate 6021 is meshed with the driven gear 901, the piston plate 8 moves to the limiting block 14 and can be limited by the limiting block 14, at the moment, the sliding doors 4 at two sides are relatively far away, the conveying channel 202 is opened, an operator can conveniently take and put glassware, and hands are prevented from being scalded;
s4: after the glass ware is placed on the steel wire mesh 603 of the placement frame 602, the electric push rod 6 is controlled to drive the placement frame 602 to reset, the rack plate 6021 is meshed with the driven gear 901 again, the rotary rod 9 and the screw rod 11 rotate reversely, the rotary rod 9 releases the pull rope 902, the screw rod 11 drives the sliding door 4 to reset through the sleeve 111 to block the conveying channel 202 again, after the sliding door 4 resets, the accommodating groove 401 of the sliding door 4 is opposite to the limiting block 14, at the moment, the limiting block 14 is not extruded by the sliding door 4 any more, the limiting block 14 resets under the action of the second elastic element 1311, the limiting block 14 moves out of the limiting hole 802, the piston plate 8 in the ventilation cavity 7 resets under the pull of the first elastic element 801, the second air cavity 702 extracts air mixed with the outside in the conveying channel 202 through the second air inlet valve, the first air cavity 701 discharges the extracted clean air into the conveying channel 202 through the first air outlet valve, air conversion in the conveying channel 202 is completed in the process of conveying the glass ware, and then the placement frame 602 is propped against the partition board 5 again, so that the placement frame 602 is overturned along with the electric push rod 6 and enters the operation cavity 201 again;
s5: the staff performs an observation operation on stem cells in the glassware through the bacteria-isolating operation glove 161 at the perspective window 16.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (5)
1. The sterile operation device for stem cell preparation comprises a support column (101) and a sterile base (1) arranged at the top of the support column (101), and is characterized in that the upper end of the sterile base (1) is connected with an operation shell (2), the top of the operation shell (2) is connected with a circulating bellows (3), an operation cavity (201) and a conveying channel (202) which are mutually communicated are arranged in the operation shell (2), a pushing mechanism is arranged in the operation cavity (201), a partition component used for partitioning the operation cavity (201) and the conveying channel (202) is arranged on the inner wall of the conveying channel (202), two groups of sliding doors (4) are connected with the front end of the operation shell (2) in a sliding mode, a ventilation mechanism used for replacing air in the conveying channel (202) is arranged between the sliding doors (4) and the partition component, and the ventilation mechanism is connected with a transmission component used for driving the sliding doors (4) to displace;
the partition assembly comprises two partition plates (5) which are movably abutted, the partition plates (5) are rotatably connected in the conveying channel (202) through rotating shafts, torsion springs are sleeved on the rotating shafts, sealing sleeves (501) are sleeved on the outer sides of the partition plates (5), and the sealing sleeves (501) are movably abutted against the inner walls of the conveying channel (202);
the pushing mechanism comprises an electric push rod (6) fixedly arranged on the inner wall of the operation cavity (201), an arc-shaped groove (502) matched with the electric push rod (6) is formed in the partition board (5), one end, far away from the inner wall of the operation cavity (201), of the electric push rod (6) is connected with a mounting plate (601), a placement frame (602) is arranged on the mounting plate (601), the placement frame (602) is movably abutted against the partition board (5), and a steel wire mesh (603) for placing glassware is arranged in the placement frame (602);
the air exchange mechanism comprises an air exchange assembly and a driving assembly for driving the air exchange assembly to act, the air exchange assembly comprises air exchange cavities (7) arranged on two sides of an operation shell (2), the two air exchange cavities (7) are provided with a first air cavity (701) and a second air cavity (702), a piston plate (8) is slidably connected in each air exchange cavity (7), a first elastic element (801) is arranged between the piston plate (8) and the inner wall of the air exchange cavity (7), a first air inlet valve and a first air outlet valve are arranged on the first air cavity (701), the first air cavity (701) is communicated with the operation cavity (201) through the first air inlet valve, the first air cavity (701) is connected with a conveying channel (202) through the first air outlet valve, a second air inlet valve and a second air outlet valve are arranged on the second air cavity (702) and are connected with the conveying channel (202) through the second air inlet valve, and the second air cavity (702) is communicated with the outside atmosphere through the second air outlet valve;
the driving assembly comprises a rotating rod (9) which is rotatably arranged in the operating shell (2), a driven gear (901) is arranged on the rotating rod (9), a rack plate (6021) which is in meshed connection with the driven gear (901) is arranged at the bottom of the placing frame (602), a pull rope (902) is sleeved outside the rotating rod (9), and one end, far away from the rotating rod (9), of the pull rope (902) penetrates through the air exchanging cavity (7) and is connected with the piston plate (8);
the transmission assembly comprises a screw rod (11) which is rotatably arranged in an operation shell (2), synchronous wheels (10) are arranged on the screw rod (11) and a rotating rod (9), synchronous belts are arranged between the synchronous wheels (10), sleeves (111) are connected to the outer sides of the screw rod (11) in a threaded mode, the sleeves (111) are fixedly connected with a sliding door (4), and sliding grooves (12) used for sliding of the sleeves (111) are formed in the operation shell (2).
2. The sterile handling device for stem cell preparation according to claim 1, wherein the inner wall of the delivery channel (202) is provided with an ultraviolet germicidal lamp (15), and wherein the inner wall of the delivery channel (202) is provided with an arc-shaped flow guiding surface.
3. The sterile operation device for stem cell preparation according to claim 2, characterized in that a movable groove (13) which is mutually communicated with the ventilation cavity (7) is formed in the operation shell (2), a limiting block (14) is connected in the movable groove (13) in a sliding manner, a limiting hole (802) which is matched with the limiting block (14) is formed in the piston plate (8), a containing groove (401) which is matched with the limiting block (14) is formed in the sliding door (4), the limiting block (14) comprises a first block body (141), a second block body (142) and a third elastic element (143) which is connected with the first block body (141) and the second block body (142), a sliding groove (131) which is matched with the sliding block (1411) is formed in the inner wall of the movable groove (13), and a second elastic element (1311) is arranged between the inner wall of the sliding groove (131) and the sliding block (1411).
4. The sterile operation device for stem cell preparation according to claim 1, wherein a perspective window (16) is inlaid on the operation shell (2), and a bacteria-isolating operation glove (161) is arranged on the perspective window (16).
5. A method of using the sterile handling device for stem cell preparation of any one of claims 1-4, comprising the steps of:
s1: when the device is used, the circulating bellows (3) works to build a sterile environment in the operation shell (2), the electric push rod (6) is controlled to start, the electric push rod (6) drives the placement frame (602) to move into the conveying channel (202) from the operation cavity (201) through the mounting plate (601), in the process, the placement frame (602) props against the partition plate (5) and acts force, the partition plate (5) is stressed to deflect by taking the rotating shaft as the center, after the electric push rod (6) drives the placement frame (602) to pass over the partition plate (5), the partition plate (5) is reset to rotate under the action of the torsion spring, the two partition plates (5) prop against again, the arc-shaped grooves (502) on the two partition plates (5) adapt to the telescopic parts of the electric push rod (6), and the partition plate (5) cuts off the operation cavity (201) from the conveying channel (202);
s2: along with the electric push rod (6) driving the placing frame (602) to move continuously in the conveying channel (202), a rack plate (6021) on the placing frame (602) is meshed with a driven gear (901) on the rotating rod (9), the driven gear (901) drives the rotating rod (9) to rotate and wind up a pull rope (902), when the pull rope (902) is wound up, the piston plate (8) is driven to slide in the ventilation cavity (7), the first elastic element (801) is deformed, the piston plate (8) in the first air cavity (701) draws clean air in the operation cavity (201) and the circulating bellows (3) through a first air inlet valve, and the piston plate (8) in the second air cavity (702) discharges air in the second air cavity (702) through a second air outlet valve;
s3: when the rotating rod (9) rotates initially, one end of the limiting block (14) is arranged in a containing groove (401) of the sliding door (4), after the rotating rod (9) rotates, the screw rod (11) rotates along with the rotating rod (9) through the synchronous wheel (10) and the synchronous belt, the sleeve (111) sleeved on the outer side threads of the screw rod (11) can drive the sliding door (4) to displace when the screw rod (11) rotates, the sliding door (4) extrudes the limiting block (14) to enable the limiting block (14) to move into the ventilation cavity (7), when the rack plate (6021) is meshed with the driven gear (901), the piston plate (8) moves to the limiting block (14) and can be limited by the limiting block (14), and at the moment, the sliding doors (4) at two sides are relatively far away, the conveying channel (202) is opened, so that an operator can conveniently take and put a glass vessel, and hands are prevented from being scalded;
s4: after the glass ware is placed on the steel wire mesh (603) of the placement frame (602), the electric push rod (6) is controlled to drive the placement frame (602) to reset, the rack plate (6021) is meshed with the driven gear (901) again, the rotary rod (9) and the screw rod (11) are reversely rotated, the rotary rod (9) releases the pull rope (902), the screw rod (11) drives the sliding door (4) to reset through the sleeve (111) so as to reseal the conveying channel (202), after the sliding door (4) is reset, the accommodating groove (401) of the sliding door (4) is opposite to the limiting block (14), at the moment, the limiting block (14) is not extruded by the sliding door (4), the limiting block (14) is reset under the action of the second elastic element (1311), the limiting block (14) moves out of the limiting hole (802), the piston plate (8) in the ventilation cavity (7) is reset under the pulling of the first elastic element (801), the second air cavity (702) extracts air in the conveying channel (202) through the second air inlet valve, and the air in the conveying channel (202) is in contact with the outside, the first air cavity (701) pumps the extracted air through the first air outlet valve (202), the air outlet valve (202) is pushed out of the conveying channel (202), the glass ware is completely and then placed in the conveying channel (5) in a turnover mode, and the partition plate (5) is completely placed in the process, the placement frame (602) enters the operation cavity (201) along with the electric push rod (6) again;
s5: the staff observe and operate the stem cells in the glassware through the bacteria-isolation operation glove (161) at the perspective window (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310120838.3A CN116273251B (en) | 2023-02-14 | 2023-02-14 | Sterile operation device for stem cell preparation and application method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310120838.3A CN116273251B (en) | 2023-02-14 | 2023-02-14 | Sterile operation device for stem cell preparation and application method thereof |
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| CN116273251B true CN116273251B (en) | 2023-08-25 |
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| CN116273251A (en) | 2023-06-23 |
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