CN207079182U - A kind of column chromatography device of immunoglobulin - Google Patents
A kind of column chromatography device of immunoglobulin Download PDFInfo
- Publication number
- CN207079182U CN207079182U CN201721062942.8U CN201721062942U CN207079182U CN 207079182 U CN207079182 U CN 207079182U CN 201721062942 U CN201721062942 U CN 201721062942U CN 207079182 U CN207079182 U CN 207079182U
- Authority
- CN
- China
- Prior art keywords
- purifying tank
- column chromatography
- chromatography device
- immunoglobulin
- feed tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model discloses a kind of column chromatography device of immunoglobulin, including purifying tank, feed tube is installed at the top of the purifying tank, constant flow pump is installed on the feed tube, the feed tube connects through the top at the top of purifying tank and chromatographic column, the inwall of the purifying tank is provided with ultraviolet germicidal lamp, the bottom of the chromatographic column is provided with flow valve, the bottom of the flow valve is connected by drain pipe with the left side of Protein Detection instrument, and the utility model only needs the liquid mobilization dynamic of a whole chromatographic apparatus of constant flow pump can completion.The Protein Detection instrument of offer can be monitored in real time to the purge process of albumen, and the layering of layer, cellulose dialysis layer and affine analysis layer is analysed by gel filtration, improved the degree of purity of immunoglobulin, be adapted to promote.
Description
Technical field
It the utility model is related to immunoglobulin technical field, the column chromatography dress of specially a kind of immunoglobulin
Put.
Background technology
The protein component essential as composition human body, it is described as the living matter of human body, the protein of nature
As the supplement of human body and intake essentially from animal meat and cereal etc., for extracting protein from waste, have become
Biochemical field produces a big trend of protein, and protein will because of its expensive process that production is also purified to it
Ask strict, and yield is extremely low, increasingly can not meet the demand in market.
In the prior art, protein detection and its purification are always the one of life science and its medical research field to grind greatly
Problem is studied carefully, because it is widely used in modern biology research, drug monitoring, agri-scientific research, chemical industry, food and its medical institutions.
Current protein purification device is not that purification effect is bad, is exactly that purification devices are excessively complicated, causes to purify cost height, operation is multiple
Miscellaneous, repeatability is poor, therefore, proposing a kind of column chromatography device of immunoglobulin.
The content of the invention
The purpose of this utility model is the column chromatography device for providing a kind of immunoglobulin, to solve above-mentioned background
The problem of being proposed in technology.
To achieve the above object, the utility model provides following technical scheme：A kind of column chromatography of immunoglobulin
Device, including purifying tank, feed tube is installed at the top of the purifying tank, constant flow pump is installed on the feed tube, it is described enter
Liquid pipe connects through the top at the top of purifying tank and chromatographic column, and the inwall of the purifying tank is provided with ultraviolet germicidal lamp, institute
The bottom for stating chromatographic column is provided with flow valve, and the bottom of the flow valve is connected by drain pipe with the left side of Protein Detection instrument.
Preferably, the chromatographic column is clear glass post, and the top of chromatographic column is provided with feed hopper.
Preferably, the bottom four corners of the purifying tank are symmetrically provided with supporting leg.
Preferably, the outside of the ultraviolet germicidal lamp is socketed with transparent glass cover, the quantity of the ultraviolet germicidal lamp
20 must not be less than, and ultraviolet germicidal lamp is equidistant circle distribution.
Preferably, the inside of the chromatographic column be sequentially installed with from top to bottom gel filtration analysis layer, cellulose dialysis layer and
Affine analysis layer.
Preferably, the right-hand member of the purifying tank is installed with dsp controller, the constant flow pump, ultraviolet germicidal lamp, egg
White detector and flow valve are electrically connected with dsp controller.
Compared with prior art, the beneficial effects of the utility model are：The utility model only needs a constant flow pump can
To complete the liquid mobilization dynamic of whole chromatographic apparatus.The Protein Detection instrument of offer can carry out real-time to the purge process of albumen
Monitoring, the layering of layer, cellulose dialysis layer and affine analysis layer is analysed by gel filtration, improves the pure of immunoglobulin
Degree, it is adapted to promote.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is top view of the present utility model；
Fig. 3 is circuit module schematic diagram of the present utility model.
In figure：It is 1 purifying tank, 2 feed tubes, 3 constant flow pumps, 4 chromatographic columns, 5 feed hoppers, 6 supporting legs, 7 ultraviolet germicidal lamps, 8 saturating
Bright cloche, 9 gel filtrations analysis layer, 10 cellulose dialysis layers, 11 affine analysis layers, 12 Protein Detection instrument, 13 flow valves, 14 go out liquid
Pipe, 15 dsp controllers.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out
Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole
Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made
The every other embodiment obtained, belong to the scope of the utility model protection.
Fig. 1-3 are referred to, the utility model provides a kind of technical scheme：The column chromatography device of the immunoglobulin,
Including purifying tank 1, the top of the purifying tank 1 is provided with feed tube 2, is provided with constant flow pump 3 on the feed tube 2, it is described enter
Liquid pipe 2 is connected through the top of purifying tank 1 with the top of chromatographic column 4, and the inwall of the purifying tank 1 is provided with ultraviolet germicidal lamp
7, the bottom of the chromatographic column 4 is provided with flow valve 13, and the bottom of the flow valve 13 passes through drain pipe 14 and Protein Detection instrument 12
Left side connection.
Specifically, the chromatographic column 4 is clear glass post, and the top of chromatographic column 4 is provided with feed hopper 5.
Specifically, the bottom four corners of the purifying tank 1 are symmetrically provided with supporting leg 6.
Specifically, the outside of the ultraviolet germicidal lamp 7 is socketed with transparent glass cover 8, the number of the ultraviolet germicidal lamp 7
Must not measure less than 20, and ultraviolet germicidal lamp 7 is equidistant circle distribution.
Specifically, the inside of the chromatographic column 4 is sequentially installed with gel filtration analysis layer 9, cellulose dialysis layer from top to bottom
10 and it is affine analysis layer 11.
Specifically, the right-hand member of the purifying tank 1 is installed with dsp controller 15, the constant flow pump 3, ultraviolet sterilization
Lamp 7, Protein Detection instrument 12 and flow valve 13 are electrically connected with dsp controller 15.
Specifically, in use, startup ultraviolet germicidal lamp 7, carries out sterilizing to the inside for purifying tank 1, avoids other
Pollution of the bacterium to immunoglobulin, sterilizing closes ultraviolet germicidal lamp 7 after terminating, it is only necessary to which a constant flow pump 3 is just
The liquid mobilization dynamic of whole chromatographic apparatus can be completed.The Protein Detection instrument 12 of offer can be carried out to the purge process of albumen
Monitoring in real time, the diameter in its bottom cross section is more than by the diameter in the top cross section of feed hopper 5, improves immunoglobulin
Blanking velocity, the layering of layer 9, cellulose dialysis layer 10 and affine analysis layer 11 is analysed by gel filtration, improves immune globulin
White degree of purity.
While there has been shown and described that embodiment of the present utility model, for the ordinary skill in the art,
It is appreciated that these embodiments can be carried out with a variety of changes in the case where not departing from principle of the present utility model and spirit, repaiied
Change, replace and modification, the scope of the utility model are defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of column chromatography device of immunoglobulin, including purifying tank（1）, it is characterised in that：The purifying tank（1）'s
Top is provided with feed tube（2）, the feed tube（2）On constant flow pump is installed（3）, the feed tube（2）Through purifying tank
（1）Top and chromatographic column（4）Top connection, the purifying tank（1）Inwall be provided with ultraviolet germicidal lamp（7）, the layer
Analyse post（4）Bottom be provided with flow valve（13）, the flow valve（13）Bottom pass through drain pipe（14）With Protein Detection instrument
（12）Left side connection.
A kind of 2. column chromatography device of immunoglobulin according to claim 1, it is characterised in that：The chromatographic column
（4）For clear glass post, and chromatographic column（4）Top be provided with feed hopper（5）.
A kind of 3. column chromatography device of immunoglobulin according to claim 1, it is characterised in that：The purifying tank
（1）Bottom four corners be symmetrically provided with supporting leg（6）.
A kind of 4. column chromatography device of immunoglobulin according to claim 1, it is characterised in that：The ultraviolet
Bactericidal lamp（7）Outside be socketed with transparent glass cover（8）, the ultraviolet germicidal lamp（7）Quantity must not be less than 20, and
Ultraviolet germicidal lamp（7）For equidistant circle distribution.
A kind of 5. column chromatography device of immunoglobulin according to claim 1, it is characterised in that：The chromatographic column
（4）Inside be sequentially installed with from top to bottom gel filtration analysis layer（9）, cellulose dialysis layer（10）With affine analysis layer（11）.
A kind of 6. column chromatography device of immunoglobulin according to claim 1, it is characterised in that：The purifying tank
（1）Right-hand member be installed with dsp controller（15）, the constant flow pump（3）, ultraviolet germicidal lamp（7）, Protein Detection instrument（12）
And flow valve（13）And dsp controller（15）It is electrically connected with.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721062942.8U CN207079182U (en) | 2017-08-24 | 2017-08-24 | A kind of column chromatography device of immunoglobulin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721062942.8U CN207079182U (en) | 2017-08-24 | 2017-08-24 | A kind of column chromatography device of immunoglobulin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207079182U true CN207079182U (en) | 2018-03-09 |
Family
ID=61420707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721062942.8U Expired - Fee Related CN207079182U (en) | 2017-08-24 | 2017-08-24 | A kind of column chromatography device of immunoglobulin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207079182U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111558354A (en) * | 2020-05-12 | 2020-08-21 | 南京至泰生物医药科技有限公司 | Purification and filtration device for biochemical reaction |
-
2017
- 2017-08-24 CN CN201721062942.8U patent/CN207079182U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111558354A (en) * | 2020-05-12 | 2020-08-21 | 南京至泰生物医药科技有限公司 | Purification and filtration device for biochemical reaction |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180309 Termination date: 20210824 |