CN105624251B - A kind of peony seeds blood sugar reducing peptide and its purification process and application - Google Patents
A kind of peony seeds blood sugar reducing peptide and its purification process and application Download PDFInfo
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- CN105624251B CN105624251B CN201610134873.0A CN201610134873A CN105624251B CN 105624251 B CN105624251 B CN 105624251B CN 201610134873 A CN201610134873 A CN 201610134873A CN 105624251 B CN105624251 B CN 105624251B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
The invention discloses a kind of peony seeds blood sugar reducing peptide, preparation method is as follows：(1) peony seeds skimmed milk is uniformly mixed with water, and ultrasonic agitation extracts albumen, centrifugation, and gained precipitating repeats to extract twice, is merged supernatant liquid and is adjusted pH to 4~4.5 using 1mol/L HCl, centrifugation takes precipitating to be freeze-dried, obtains peony seeds albumen；(2) 1~3% peony seeds protein solution is prepared, is digested using trypsase ultrasonic wave added, reaction terminates, and 10~15min of boiling water bath carries out enzyme deactivation, and peony seeds blood sugar reducing peptide can be obtained.Present invention further proposes the purification process of above-mentioned peony seeds blood sugar reducing peptide and applications.The present invention utilizes ultrasonic wave auxiliary biological enzyme degradation peony seeds albumen, it is cut into a variety of different molecular weight polypeptides, then it is isolated and purified using ultrafiltration, ion exchange, sephadex and high performance liquid chromatography, it not only can efficiently carry out endonuclease reaction, obtain a large amount of peony seeds blood sugar reducing peptide, and it is able to maintain polypeptide active in purification process not lose, utilization easy to produce.
Description
Technical field
The invention belongs to technical field of food biotechnology, and in particular to a kind of peony seeds blood sugar reducing peptide and its purification process and answer
With.
Background technique
Diabetes are a kind of diseases for seriously threatening human health.With the rapid development of economic level, the diet of people
It becomes better and better, amount of exercise is reduced increasingly, is led diabetogenic disease incidence and is increased year by year.Traditional hypoglycemic agent includes glucoside
Enzyme inhibitor, insulin sensitizer, Insulin secretagogues, Chinese patent drug, insulin, biguanides, sulfonephthalein carbamide type medicine etc.,
Since there are side effects for drug therapy, patient is used for a long time and is difficult to receive.In recent years, reach the method for control blood glucose through diet
It increasingly has been favored by people, wherein developing the striving direction that new alpha-glucosidase restrainer is numerous scholars.
There is scholar using albumen such as mottle clam, silk, egg white and wheys as raw material recently, enzymatic hydrolysis preparation, which has, inhibits α-grape
The active peptide of glycosidase.But the activity of these polypeptides is not high, as two tripeptides isolated in the blood sugar lowing polypeptide of silk source, half press down
Amount of formulation is respectively 2.7mg/mL and 1.5mg/mL.Therefore, the blood sugar reducing peptide for researching and developing novel high-activity is that industry emphasis will solve
Problem certainly.
Since peony seed oil becomes edible oil by Ministry of Public Health's official approval, China's oil with the cultivated area of tree peony by
Year is incremented by, and the peony seeds dregs of rice yield after oil expression is also continuously increased, and wherein crude protein accounts for about 28.34% (W/W) of the peony seeds dregs of rice.It is male
Red seed albumen contains 18 kinds of natural amino acids, wherein 8 kinds of human bodies must amino acid account for 30.44%, nutritive proportion is reasonable, has pole
Big development prospect.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of peony seeds blood sugar reducing peptide and its purification process, using ultrasound
Wave assistance enzymolysis peony seeds albumen, is isolated and purified by ultrafiltration, ion exchange resin, sephadex, obtains high activity
Blood sugar reducing peptide.It is not only the comprehensive utilization offer new method of peony seeds, even more important is to obtain a kind of novel, high activity drop
Blood glucose peptide brings glad tidings for numerous diabetics.
Technical solution：To realize the above-mentioned technical purpose, peony seeds blood sugar reducing peptide of the invention is prepared via a method which
It arrives：
(1) by the peony seeds skimmed milk and water that are obtained after peony seeds oil expression according to solid-liquid ratio 1:8~12 are uniformly mixed, and adjust
PH to 9~9.5 is saved, ultrasonic agitation extracts albumen at 40~60 DEG C, and then 3500~3800r/min is centrifuged 10~15min, takes
Layer liquid, resulting precipitating are repeated to extract twice according to the above method, merge resulting supernatant liquid, adjusted using 1mol/L HCl
PH to 4~4.5,3500~3800r/min are centrifuged 10~15min, take precipitating to be freeze-dried to get peony seeds albumen；
(2) prepare 1~3% peony seeds protein solution, using trypsin digestion, according to add trypsase 5000~
125000U/g, pH7.5~8.5,35~40 DEG C of temperature, 15~25min of time of ultrasonic wave added enzymatic hydrolysis, ultrasonic frequency 22~
40kHz, 50~200W/L of power, reaction terminate, and 10~15min of boiling water bath carries out enzyme deactivation, and peony seeds blood sugar reducing peptide can be obtained.
In a preferred embodiment, it is prepared via a method which to obtain peony seeds blood sugar reducing peptide solution：
A. raw material and distilled water are mixed, adjusts pH value of solution to 9.5,50 DEG C of water bath sonicator intervals using the NaOH of 1mol/L
Albumen 2h is extracted in stirring, and 3800r/min is centrifuged 15min, and gained precipitating is extracted twice again, and gained supernatant liquid uses 1mol/L
HCl adjusts pH to 4.5,3800r/min and is centrifuged 15min, takes precipitating to be freeze-dried, can be obtained peony seeds albumen.
B. 1~3% peony seeds protein solution is prepared, using trypsase according to adding 5000~125000U/g of trypsase,
PH7.5~8.5,35~40 DEG C of temperature, 15~25min of time of ultrasonic wave added enzymatic hydrolysis, 22~40kHz of ultrasonic frequency, power
50~200W/L, reaction terminate, and boiling water bath 10min carries out enzyme deactivation, and peony seeds polypeptide solution can be obtained.
Present invention further proposes the purification process of above-mentioned peony seeds blood sugar reducing peptide, which is characterized in that including walking as follows
Suddenly：
(1) 8000~10000r/min of peony seeds blood sugar reducing peptide is centrifuged 15~20min, takes middle layer supernatant liquid, first makes
With 0.46 μm or 0.22 μm of membrane filtration, ultrafiltration is carried out after filtering, first initial filter is carried out using the ultrafiltration membrane of 10000Da, is divided
Son amount carries out second ultrafiltration using the filter membrane of 5000Da in 10000Da primary filtrate below, then by initial filter solution, is finally pressed down
The best molecular weight of rate processed is in 5000Da or less filtrate；
(2) ion exchange adsorption is carried out using resin, Static Adsorption 1h or more collects eluate and is freeze-dried, obtained
Polypeptide is freeze-dried to level-one；
(3) the freeze-drying polypeptide that step (2) obtains is configured to aqueous solution, column layer is carried out using Sephadex G-15
Analysis, the sample collected and freeze-drying obtain second level freeze-drying polypeptide；
(4) obtained second level freeze-drying polypeptide is used into C-18 reverse-phase chromatography post separation, obtains peony seeds after purification
Blood sugar reducing peptide.
Preferably, in step (2), the resin be 732 type resin cations, D151 large hole cation exchanger resin,
Any one in 717 type anion exchange resin.It is highly preferred that using 732 type adsorption rate highests, the best of effect
Specifically, it is using the preferred steps that 732 types adsorb：The 732 sodium form resin cations handled well are packed into and are chromatographed
Column is balanced using distilled water with flow velocity 2%CV/min, is detected using UV detector in 220nm in downstream, until
Baseline is steady, and 5000kD ultrafiltration solution is added and flows through resin bed, until also become red below resin, using distilled water with flow velocity
2%CV/min rinses resin, and 220nm baseline is steady, uses 0.5~2% (v/v) ammonium hydroxide instead and is washed according to 2%CV/min
De-, 220nm detection is collected second eluting peak and is freeze-dried, wherein CV indicates actual column volume product.
Preferably, in step (3), use Sephadex G-15 carry out column chromatography the step of for：By what is handled well
Sephadex G-15 is packed into chromatographic column, and diameter height is than 1:30~1:45, it is balanced using distilled water according to 2%CV/min flow velocity,
It is detected using UV detector in 220nm in downstream；The polypeptide solution loading that will be prepared, volume be column volume 5~
10%, it to sample completely into gel column bed, is rinsed using distilled water according to 2%CV/min, 220nm is detected and collected second
Eluting peak, CV indicate actual column volume product.
Preferably, the step of C-18 reverse-phase chromatography post separation in step (4) is：Prepare mobile phase, wherein mobile phase A
For according to volume ratio ultrapure water：TFA=99.95:0.05, Mobile phase B is according to volume ratio acetonitrile：TFA=99.95:0.05, stream
Dynamic phase A, B uses 0.22 μm of membrane filtration, according to volume ratio mobile phase A：Mobile phase B=85:15, flow velocity 12%CV/min,
Detection wavelength 220nm is detected and is collected, and CV indicates actual column volume product.
Closer, the invention proposes above-mentioned peony seeds blood sugar reducing peptide or purified obtained tree peonies after purification
Seed blood sugar reducing peptide is in preparation for the application in hypoglycemic drug or health care product.
Beneficial effect：Compared with prior art, the invention has the advantages that：
(1) present invention carries out ultrasonic wave complex enzyme hydrolysis by the peony seeds dregs of rice after extracting oil to peony seeds, and is separated
Purifying, prepares novel, high activity peony seeds blood sugar lowing polypeptide.The reaction time that traditional enzymolysis protein matter needs is long, and efficiency
Lowly, time-consuming and laborious.The present invention uses ultrasound-assisted enzymolysis, not only may be implemented to shorten the reaction time, and can increase
Efficiency of pcr product.
(2) hyperfiltration technique is a kind of using pressure as the membrane separation technique of motive force, will be big according to the size of molecular weight of material
Molecule is separated with the substance of small molecule, simple and convenient, low in cost, do not increase any chemical reagents, do not cause macromolecular
The deactivation of substance, and have many advantages, such as that packing density is high in unit container, occupied area is small.The application uses ultrafiltration skill
Art guarantees the activity after polypeptide initial gross separation to greatest extent.
(3) 732 type cation exchange resins have processing capacity big, can be used with repeated regeneration, long working life, run
The more low advantage of expense, is suitble to industrial production demand, D151 large hole cation exchanger resin have aperture and large specific surface area,
The advantages that adsorption capacity is big, selectivity is good, adsorption rate is fast, desorption condition is mild, regeneration treatment is convenient, service life is long, it is full
Sufficient industrial production needs.There is 717 type anion exchange resin regeneration efficiency height, buck to consume, and low, exchange capacity is big, anti-organic matter
Pollution and the advantages that oxidation resistance is strong, mechanical strength is good, is suitble to demand of industrial production.Sephadex series is led to by glucan
The three-dimensional netted gel particle that epoxychloropropane is cross-linked to form is crossed, wherein the molecular weight of G-15 separate substance is less than 1500Da,
It is suitable for desalination, the separation of peptide and other small molecules in this molecular weight ranges.Advantage is that Sephadex G-15 belongs to inertia
Carrier, neutral, adsorption capacity is weak, and operating condition is very mild, has good guarantor to physicochemical property, the activity of separation composition
It holds.Separation and the active advantage kept mainly are carried out according to molecular size range using its mild condition here.
(4) method separated in the present invention using efficient liquid phase, can prepare the higher target product polypeptide of purity, pole
Has application prospect.China is going into aging society at present, and diabetes are high morbidity and the commonly-occurring disease of elderly population, and have
To the trend strided forward that becomes younger, therefore for reduce blood glucose based food research and development it is very urgent.Biological industry flourishes,
There is good facilitation for the popularization of peony seeds blood sugar reducing peptide, capsule, tablet or beverage, drink class is made in following process
Equal products.
Detailed description of the invention
Fig. 1 is that the preparation of peony seeds blood sugar reducing peptide of the present invention purifies flow diagram；
Fig. 2 is the ultraviolet detection schematic diagram of the polypeptide solution after 732 sodium form cationic resin columns chromatography；
Fig. 3 is the ultraviolet detection schematic diagram of the polypeptide solution obtained after Sephadex G-15 column chromatographs；
Fig. 4 is the HPLC schematic diagram of C-18 reverse-phase chromatographic column polypeptide solution obtained after separation.
Specific embodiment
As shown in Figure 1, the present invention provides a kind of peony seeds blood sugar reducing peptide and its purification process, including peony seeds albumen
Preparation, the preparation of polypeptide enzymolysis liquid, and polypeptide of the molecular weight less than 5000 is further collected by ultrafiltration, then successively use ion
Exchange resin, sephadex separation and liquid phase separation, the peony seeds blood sugar reducing peptide purified.
Below by specific embodiment, the present invention will be described in detail.Wherein, in following embodiments, CV indicates that actual column holds
Volume；Ammonia concn is volume fraction (V/V).
Embodiment 1
By raw material and distilled water according to solid-liquid ratio 1:10 are matched, and stirring 15min mixes well it, use 1mol/L
NaOH adjusts pH value of solution and is centrifuged 15~20min to 9.5,50 DEG C of water bath sonicators 2h, 3800r/min, and gained precipitating extracts two again
It is secondary, merge gained supernatant liquid using 1mol/L HCl adjust pH to 4.5,3800r/min be centrifuged 15~20min, take precipitate into
Row freeze-drying, can be obtained peony seeds albumen.
Embodiment 2~13
Reaction conditions are：Compound concentration peony seeds protein solution 1~3%, 5000~12500U/g of enzyme concentration,
PH7.5~8.5,35~40 DEG C of temperature, 15~25min of time, ultrasonic frequency 28kHz, power 100W/L, enzyme digestion reaction terminate
Peony seeds polypeptide enzymolysis liquid can be obtained in boiling water bath 10min afterwards.
External hypoglycemic measuring method：
(1) prepared by mouse alpha-glucosidase
Mouse is put to death in dislocation, washes away sundries, liquid nitrogen grinding with 0 DEG C of physiological saline.PBS is mixed with grounds travel, according to body
Product mass ratio 10:1 proportion, extraction of ocean eddies 60s, 4 DEG C of 8000r/min centrifugation 15min take supernatant.
(2) alpha-glucosaccharase enzyme inhibition rate detection method
Light absorption value is detected at water-bath 37 DEG C of reactions 15min, 405nm；
Inhibiting rate calculation method is as follows：
(3) reagent needed for
PBS：0.05mol/L pH6.8 phosphate buffer
PNPG solution：The pNPG (p-nitrophenol-α-D- glucopyranoside) for accurately weighing 50mg, is dissolved in 10mLPBS.
Enzyme solutions：Mouse enzyme solutions are extracted in 1.6mLPBS+0.2mLpNPG+0.2mL enzyme solutions reaction system, water
Bathe 37 DEG C of reactions 15min, OD405Between 0.4~0.45.
Testing result is as shown in table 1：
Blood sugar reducing peptide activity under the different preparation conditions of table 1
The inhibitory effect of 13 gained enzymolysis polypeptide liquid of embodiment is best known to upper table 1, therefore selection example 13 carries out
Further isolate and purify.
Embodiment 14
Polypeptide enzymolysis liquid 8000r/min obtained by embodiment 13 is centrifuged 15min, takes middle layer supernatant liquid, 0.46 μm of filter
Film filters, and is carrying out ultrafiltration using ultrafiltration apparatus after membrane filtration, first using the ultrafiltration membrane progress initial filter of 10000Da, then will
Initial filter solution carries out second ultrafiltration using the filter membrane of 5000Da, it is final choose best 5000Da or less the filtrate of inhibiting rate save to
With；
Embodiment 15~17
By the 732 type resin cations handled well, D151 large hole cation exchanger resin, 717 type anion exchange resin
Static Adsorption is carried out respectively, measures adsorption rate respectively after absorption 1h, as shown in table 2.
The Static Adsorption rate of 2 different type resin of table
Embodiment 18~21
It weighs the 732 type resin cations that 1g is handled well and is packed into chromatographic column, 3CV is rinsed using distillation water balance, in downstream
It is detected using UV detector 220nm, then 5000kD ultrafiltration solution is flowed through into resin column according to 2%CV/min, until tree
Outlet also becomes red below rouge column, is replaced with distilled water, is rinsed, and rinses resin according to 2%CV/min, 220nm is purple
Until outer detection numerical value no longer fluctuates, uses 0.5,1.0,1.5,2.0% concentration ammonium hydroxide instead and eluted as eluent, as a result such as
Shown in table 3：
The eluting rate of 3 various concentration ammonium hydroxide of table
Embodiment 22
The 732 type resin cations handled well are packed into chromatographic column, are rushed using distilled water according to flow velocity 2%CV/min flow velocity
It washes, downstream is detected using UV detector in 220nm, and the molecular weight prepared in embodiment 14 is less than 5000kD ultrafiltrate
Resin column is flowed through according to flow velocity 0.2%CV/min, as polypeptide is adsorbed, resin column becomes red, until outlet below column
Resin also reddens, and rinses resin according to flow velocity 0.2%CV/min using distilled water, rinses 220nm ultraviolet detection numerical value after 1CV
No longer fluctuate；It is adsorbed polypeptide according to flow velocity 0.2%CV/min elution using 2% concentration ammonium hydroxide, 220nm detection collects
Now such as second eluting peak in Fig. 2, it is freeze-dried, obtains lyophilized products.
Embodiment 23
The polypeptide sample being freeze-dried in embodiment 22 is configured to 50mg/mL solution；By what is handled well
SephadexG-15 is packed into chromatographic column, and it is 1 that diameter height, which compares,:40, it is balanced using distilled water according to 0.2%CV/min flow velocity,
It is detected using UV detector in 220nm in downstream；The polypeptide solution loading that will be prepared, volume are column volume 7.5%, are used
Distilled water is rinsed according to flow velocity 0.2CV/min, and 220nm is detected and collected such as second eluting peak in Fig. 3, and freezing is dry
It is dry, obtain lyophilized products.
Embodiment 24
23 lyophilized products of embodiment are configured to 25mg/mL solution for standby；According to ultrapure water, TFA (trifluoroacetic acid), acetonitrile
The ratio between volume configure mobile phase, mobile phase A ultrapure water：TFA=99.95:0.05, Mobile phase B acetonitrile：TFA=99.95:
0.05, mobile phase A, B use 0.22 μm of membrane filtration, according to the ratio between volume mobile phase A：Mobile phase B=85:15, flow velocity
12%CV/min, Detection wavelength 220nm, 30 DEG C of column temperature, C-18 reverse-phase chromatographic column is separated；Separating resulting is as shown in figure 4, receive
Collect the substance at No. 2 peaks, freeze-drying obtains blood sugar lowing polypeptide required for us, IC50For 47.256 μ g/mL.
It is more to be cut into a variety of different molecular weights using ultrasonic wave auxiliary biological enzyme degradation peony seeds albumen by the present invention
Then peptide is isolated and purified using ultrafiltration, ion exchange, sephadex and high performance liquid chromatography.It not only can be efficient
Endonuclease reaction is carried out, obtains a large amount of peony seeds blood sugar reducing peptide, and be able to maintain polypeptide active in purification process and do not lose, it can
With good Commercial cultivation.Meanwhile the present invention assists peony seeds proteolysis to prepare peony seeds blood sugar reducing peptide by ultrasonic wave, no
The comprehensive utilization that peony seeds can only be substantially increased, increase its economic value, and provides a kind of with high activity
Novel blood sugar lowing peptide.
Claims (6)
1. a kind of peony seeds blood sugar reducing peptide, which is characterized in that it is prepared via a method which to obtain：
（1）By the peony seeds skimmed milk and water that are obtained after peony seeds oil expression according to solid-liquid ratio 1:8 ~ 12 are uniformly mixed, and adjust pH
Ultrasonic agitation extracts albumen to 9 ~ 9.5,40 ~ 60 DEG C, and then 3500 ~ 3800 r/min are centrifuged 10 ~ 15min, takes supernatant liquid,
It is resulting precipitating according to the above method repeat extract twice, merge resulting supernatant liquid, using 1mol/L HCl adjust pH to 4 ~
4.5,3500 ~ 3800r/min is centrifuged 10 ~ 15min, takes precipitating to be freeze-dried to get peony seeds albumen；
（2）1 ~ 3% peony seeds protein solution is prepared, using trypsin digestion, according to adding 5000 ~ 125000U/g of trypsase,
PH7.5 ~ 8.5,35 ~ 40 DEG C of temperature, 15 ~ 25min of time of ultrasonic wave added enzymatic hydrolysis, 22 ~ 40kHz of ultrasonic frequency, power 50 ~
200W/L, reaction terminate, and 10 ~ 15min of boiling water bath carries out enzyme deactivation, and peony seeds blood sugar reducing peptide can be obtained.
2. the purification process of peony seeds blood sugar reducing peptide described in claim 1, which is characterized in that include the following steps：
（1）Peony seeds blood sugar reducing peptide is centrifuged 15 ~ 20min under 8000 ~ 10000r/min, middle layer supernatant liquid is taken, first uses
0.46 μm or 0.22 μm of membrane filtration, carry out ultrafiltration after filtering, first carry out initial filter using the ultrafiltration membrane of 10000Da, obtain molecule
Amount carries out second ultrafiltration using the filter membrane of 5000Da in 10000Da primary filtrate below, then by initial filter solution, finally obtains molecule
Amount is in 5000Da filtrate below；
（2）Ion exchange adsorption is carried out using resin, Static Adsorption 1h or more collects eluate and is freeze-dried, obtains one
Grade freeze-drying polypeptide；
（3）By step（2）Obtained freeze-drying polypeptide is configured to aqueous solution, carries out column chromatography using SephadexG-15, receives
Collect obtained sample and freeze-drying, obtains second level freeze-drying polypeptide；
（4）Obtained second level freeze-drying polypeptide is used into C-18 reverse-phase chromatography post separation, obtains peony seeds drop blood after purification
Glycopeptide.
3. purification process according to claim 2, which is characterized in that step（2）In, the resin be 732 type sun from
Subtree rouge, D151 large hole cation exchanger resin, any one in 717 type anion exchange resin.
4. purification process according to claim 2, which is characterized in that step（3）In, it is carried out using Sephadex G-15
Column chromatograph the step of be：The Sephadex G-15 handled well is packed into chromatographic column, diameter height is than 1:30~1:45, it is pressed using distilled water
It is balanced according to 2%CV/min flow velocity, is detected using UV detector in 220nm in downstream；It will be on the polypeptide solution that prepared
Sample, volume are the 5 ~ 10% of column volume, to sample completely into gel column bed, are rinsed using distilled water according to 2%CV/min,
220nm is detected and is collected second eluting peak, wherein CV indicates actual column volume product.
5. purification process according to claim 2, which is characterized in that step（4）In C-18 reverse-phase chromatography post separation
Step is：Prepare mobile phase, wherein mobile phase A is according to volume ratio ultrapure water：TFA=99.95:0.05, Mobile phase B be by
According to volume ratio acetonitrile：TFA=99.95:0.05, mobile phase A, B use 0.22 μm of membrane filtration, according to volume ratio mobile phase A：
Mobile phase B=85:15, flow velocity 12%CV/min, Detection wavelength 220nm is detected and is collected, wherein CV indicates that actual column holds
Volume.
6. after purification male that peony seeds blood sugar reducing peptide described in claim 1 or purification process as claimed in claim 2 obtain
Red seed blood sugar reducing peptide is in preparation for the application in hypoglycemic drug or health care product.
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| CN106967785B (en) * | 2017-02-27 | 2020-09-18 | 江苏科技大学 | Real-time monitoring method for process of producing hypoglycemic peptide by enzymolysis method |
| CN107836562A (en) * | 2017-11-02 | 2018-03-27 | 林峰 | A kind of peony seeds active peptide and preparation method |
| CN108998491A (en) * | 2018-08-29 | 2018-12-14 | 西南大学 | A method of extracting protein in the peony seeds dregs of rice |
| CN110257460A (en) * | 2019-05-23 | 2019-09-20 | 世堃堂(广东)生物科技有限公司 | A kind of preparation method of the tree peony protein peptides of blood pressure lowering |
| CN110218755A (en) * | 2019-05-23 | 2019-09-10 | 世堃堂(广东)生物科技有限公司 | A kind of preparation method of hypoglycemic tree peony active peptide |
| CN112795613B (en) * | 2021-04-06 | 2023-02-03 | 西北大学 | Peony seed meal-derived blood sugar-reducing polypeptide and application thereof |
| CN114907497B (en) * | 2022-06-27 | 2023-03-31 | 河南省商业科学研究所有限责任公司 | Preparation method and application of peony oligosaccharide extract |
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