CN107836562A - A kind of peony seeds active peptide and preparation method - Google Patents
A kind of peony seeds active peptide and preparation method Download PDFInfo
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- CN107836562A CN107836562A CN201711068427.5A CN201711068427A CN107836562A CN 107836562 A CN107836562 A CN 107836562A CN 201711068427 A CN201711068427 A CN 201711068427A CN 107836562 A CN107836562 A CN 107836562A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
Abstract
The invention provides a kind of preparation method of peony seeds active peptide.The preparation method is raw material using peony seeds, prepares refined peony seeds active peptide and full nutrition peony seeds active peptide.Efficiently digested using combination of enzyme preparations, reduce energy and material consumption；And the creative nutritional ingredient that with the addition of traditional Chinese medicine mulberry leaf and perilla seed, further enrich peony seeds peptide in enzyme deactivation is extracted.Particular filter auxiliary combination is used in separation process, greatly enhances refining spearation ability；Low-temperature spray drying tower in finally using, thermal activity material is protected, and middle low-temperature spray drying tower is also to be applied first in peptide technique.In addition, the preparation method raw material of the present invention is easy to get, simple production process, production cost is greatly reduced.And mass content >=80% of the refined peony seeds active peptide prepared, mass content >=30% of full nutrition peony seeds active peptide.
Description
Technical field
The invention belongs to biological technical field, and in particular to a kind of peony seeds active peptide and preparation method.
Background technology
Tree peony is Ranunculaceae, Chinese herbaceous peony platymiscium, is perennial machaka.The root of tree peony is processed into " root bark of tree peony ",
It is rare Chinese herbal medicine.Its cold nature, acrid flavour is nontoxic, enters the heart, liver, kidney three and passes through, have scattered blood stasis, purify the blood, tree peony and blood, analgesic,
The effect stimulated the menstrual flow, also reduce blood pressure, anti-inflammation the effect of.Long term usage can beneficial body lengthen the life, blood-nourishing and liver, dissipate strongly fragrant dissolving stasis, be applicable
In chloasma faciei, skin senescence, often drink qi and blood work lung, appearance are ruddy, improve menstrual disorder, dysmenorrhoea, only abnormal sweating, night sweat.It is male
Red seed is the elite crystallization of peony plant, has been passed on outside all characteristics that tree peony has in itself, and it more has oneself unique medicine
And nutritional ingredient, Paeonia suffruticosa seed are the nuts by shell and kind shell double shielding, have natural " longevity " gene.
Application number CN201610134873.0 disclose " a kind of peony seeds blood sugar reducing peptide and its purification process and application " its
Preparation method is as follows：(1) peony seeds skimmed milk is well mixed with water, ultrasonic agitation extraction albumen, centrifugation, and gained precipitation repeats to carry
Take twice, merge supernatant liquid and using 1mol/L HCl regulation pH to 4~4.5, centrifugation, take precipitation to be freeze-dried, obtain
Peony seeds albumen；(2) 1~3% peony seeds protein solution is prepared, is digested using trypsase ultrasonic wave added, reaction terminates, boiling water
Bathe 10~15min and carry out enzyme deactivation, you can obtain peony seeds blood sugar reducing peptide.Trypsase single enzyme enzyme is used in enzymolysis process
Solution, it is unfavorable for the thorough enzymolysis of protein.It is anti-phase using the absorption of resin progress ion exchange, C~18 during isolating and purifying
Chromatogram post separation, its is costly, is not suitable for industrialized production.Finally using freeze-drying rather than spray drying, it is unfavorable for
Industrial applications.
Publication number CN104041901B discloses " a kind of tree peony peptide beverage and preparation method thereof ", wherein preparing tree peony peptide step
It is rapid as follows：Dilute alkaline soak that 1 peony seeds benevolence pH value is 8.5~9.5 cleans, and washs, dries, frying obtains frying peony seeds
Benevolence；2 add water to grind to obtain peony seeds slurries；3 regulation pH values are 7.5~9.6, then add the ripe mixing protease of culture,
And make protease in peony seeds slurries mass percent be 0.6~1.2%, keeping temperature be 40~50 DEG C, control agitating paddle
Agitation revolution is 100~200r/min, and 24~48h is hydrolyzed under filtrated air, and hydrolysis is warming up to 110~120 DEG C after terminating and entered
Row high-temperature inactivation, filter cleaner, that is, tree peony peptide solution is made.Enzymolysis time is long and needs gnotobasis, to the equipment of factory
It is required that it is too high, and cost of idle time.Filtration step also simply simply lifts filter cleaner, and unused filtering agent and filter membrane enter one
Step processing.It is unfavorable for purification and the mouthfeel of tree peony peptide.
The content of the invention
An object of the present disclosure is to provide a kind of peony seeds active peptide.
Another object of the present invention is to provide the preparation method of above-mentioned active peptide.
A kind of preparation method of peony seeds active peptide, comprises the following steps：
A, peony seeds are pre-processed, carries out ultramicro grinding, crossed 200~500 mesh, add water to mix, adjust 8~9 micro- alkalization of pH
Processing, soak 18~24h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, weak acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is peony seeds albumen；
C, precipitation plus water are redissolved, heating so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 5~10, adds special enzyme preparation, enzymolysis；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, peony seeds active peptide powder is made in thick enzymolysis liquid.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, then spray drying obtains refined peony seeds active peptide powder.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition peony seeds activity
Gly-His-Lys.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step A, carry out ultramicro grinding
Enzymatic hydrolyzation can be improved, makes full use of material composition.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step B, the weak acid is vinegar
One or more in acid, lactic acid, citric acid, 8~12h of time.Using weak acid precipitating proteins, reduce strong acid and protein is sought
The destruction of material is supported, is advantageous to optimize the nutrition composition of active peptide.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step C, heating-up temperature 75
~100 degree, 15~30 minutes time.Using the method for thermal denaturation, it can be more favorable for digesting, enzymolysis is more abundant.Heat simultaneously
Carry out high-pressure homogeneous after denaturation, further improve enzymolysis efficiency.
The preparation method of peony seeds active peptide described above, it is characterised in that described step D, special enzyme preparation combination
Including flavor protease: trypsase: pepsin is 1~2: 1~2: 2~3.Research finds the enzyme of its special enzyme preparation combination
Solve the enzymolysis efficiency of the long-range about single enzyme of efficiency.
The preparation method of peony seeds active peptide described above, it is characterised in that characterized in that, described step D, enzymolysis
Condition is：Hydrolysis temperature is 40~65 DEG C, and enzymolysis time is 1.5~8h.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step step E, specific Chinese medicine
It is combined as：Mulberry leaf and perilla seed, addition are respectively the 1~2% of dry peony seeds powder solid masses.One can be entered by adding traditional Chinese medicine
Step plays the nutritive value of active peptide.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step E.Extract the bar of enzyme deactivation
Part is：It is 90~125 DEG C to extract enzyme-removal temperature, and the time is 0.5~3h.
The preparation method of peony seeds active peptide described above, it is characterised in that in described step step F1, special filtering
Auxiliary combination is：Activated carbon, perlite, diatomite, its ratio are 3~4: 1~2: 2~3.Research finds its special filtration adjuvant
The filter efficiency of the long-range about single filtering agent of filter efficiency of combination.
The preparation method of peony seeds active peptide described above, it is characterised in that be spray-dried used for middle cold nebulization
Drying tower, further retain thermal activity material, and middle low-temperature spray drying tower is to be applied first in peptide technique.
A kind of peony seeds active peptide, it is characterised in that it is made according to any of the above-described described preparation method, it is described refined male
Mass content >=80% of red seed active peptide, mass content >=30% of the full nutrition peony seeds active peptide.
The preparation method of the peony seeds active peptide of the present invention, specific Chinese traditional medicine composition mulberry leaf and perilla seed, can enter one first
The nutritional ingredient healthcare function of peony seeds active peptide prepared by step lifting.Mulberry leaf and perilla seed are as follows to the main function of body：
Mulberry leaf：Mulberry leaf are first-class functional food, can be depressured, reducing blood lipid, anti-aging, increase endurance, reduce cholesterol,
Suppress Fat Accumulation, suppress thrombus generation, suppress intestinal toxic bacterial reproduction, suppress harmful oxide generation, it is most prominent
Function is preventing and treating diabetes.Since ancient times, the traditional Chinese medical science is just the sugar of modern medicine using mulberry leaf as treatment diabetes to hypoglycemic effect
The application in TCM of disease is urinated in clinic, Japanese ancient book《Eat tea health note》Also recording mulberry leaf has improvement " drinking-water disease " i.e. modern medicine
The effect of diabetes.Studying data at home and abroad confirms that alkaloid and polysaccharide are blood sugar reducing components main in mulberry leaf.The drop of mulberry leaf
Blood glucose effect is realized by two approach：First, enzyme activity is decomposed to disaccharides by alkaloid DNJ1~DNJ
Property produce inhibitory action, so as to suppress absorption of the small intestine to disaccharide, reduce the peak value of blood glucose after food；Second, pass through mulberry leaf biology
Alkali and mulberry leaf polysaccharide promote β cells point must insulin, and insulin can promote utilization of the cell to sugar, hepatic glycogen synthesis and
Improve glycometabolism, be finally reached hypoglycemic effect.Perilla seed：Chinese medicine perilla seed is that Labiatae annual herb plant is tightly revived
Dry mature fruit.Property it is pungent, temperature.Return lung, large intestine channel.Function lowering the adverse-rising QI to resolve phlegm, it is relieving cough and asthma, relax bowel.Cure mainly uncomfortable in chest, cough
Cough, the disease such as constipation.The extract of purple perilla --- Rosmarinic acid, free radical antiphlogistic effects are dispelled with extraordinary.Performance：Obtain
Obtain U.S. FDA and be recognized as public security raw-food material.Active high, species is more, available for nutrition science food and special edible production
Product.Function：Inoxidizability, antiviral activity, antiinflammatory action, antithrombotic, platelet aggregation-against, antibacterial.
In addition to objects, features and advantages described above, the present invention also has other objects, features and advantages.
The present invention is further detailed explanation below.
Brief description of the drawings
Fig. 1：The process chart of refined peony seeds active peptide powder
Fig. 2：The process chart of full nutrition peony seeds active peptide powder
Embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
Embodiments of the invention are described in detail below, but the present invention can be limited and covered according to claim
Multitude of different ways implement.
Embodiment 1
A kind of preparation method of peony seeds active peptide, comprises the following steps：
A, peony seeds are pre-processed, carry out ultramicro grinding, crossed 400 mesh, add water to mix, adjust 8~9 micro- basifications of pH,
Soak 20h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is peony seeds albumen；
C, precipitation plus water are redissolved, is heated to 90 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation flavor protease: trypsase: pepsin is
1.5: 1.5: 2.5, enzymolysis, hydrolysis temperature is 50 DEG C, enzymolysis time 2h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition mulberry leaf and perilla seed are added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, peony seeds active peptide powder is made in thick enzymolysis liquid.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined peony seeds active peptide powder.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition peony seeds activity
Gly-His-Lys.
In described step E, the addition of mulberry leaf and perilla seed is respectively the 1.5% of dry peony seeds powder solid masses.
In described step E, enzyme-removal temperature is 90 DEG C, enzymolysis time 1h.
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 3.7:
1.5∶2。
Embodiment 2
A kind of preparation method of peony seeds active peptide, comprises the following steps：
A, dry peony seeds are pre-processed, carries out ultramicro grinding, crossed 400 mesh, add water to mix, adjusted at 8~9 micro- alkalization of pH
Reason, soak 20h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is peony seeds albumen；
C, precipitation plus water are redissolved, is heated to 90 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation flavor protease: trypsase: pepsin is
1.4: 1.6: 2.2, enzymolysis, hydrolysis temperature is 50 DEG C, enzymolysis time 2h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, peony seeds active peptide powder is made in thick enzymolysis liquid.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined peony seeds active peptide powder.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition peony seeds activity
Gly-His-Lys.
In described step E, the addition of mulberry leaf and perilla seed is respectively the 1.7% of dry peony seeds powder solid masses.
In described step E, enzyme-removal temperature is 95 DEG C, enzymolysis time 1h.
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 3.5:
1.8∶2。
Embodiment 3
A kind of preparation method of peony seeds active peptide, comprises the following steps：
A, dry peony seeds are pre-processed, carries out ultramicro grinding, crossed 400 mesh, add water to mix, adjusted at 8~9 micro- alkalization of pH
Reason, soak 22h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, acetic acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, from
The heart, collects precipitation, and precipitation is peony seeds albumen；
C, precipitation plus water are redissolved, is heated to 95 degree so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 7~8, adds special enzyme preparation flavor protease: trypsase: pepsin is
1.6: 1.6: 2.4, enzymolysis, hydrolysis temperature is 55 DEG C, enzymolysis time 1.5h；
E, after enzymolysis terminates, specific Chinese traditional medicine composition mulberry leaf and perilla seed are added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, peony seeds active peptide powder is made in thick enzymolysis liquid.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, is then spray-dried to obtain refined peony seeds active peptide powder.
The preparation method of peony seeds active peptide described above, it is characterised in that F-step also includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition peony seeds activity
Gly-His-Lys.
In described step E, the addition of mulberry leaf and perilla seed is respectively the 1.7% of dry peony seeds powder solid masses.
In described step E, enzyme-removal temperature is 100 DEG C, enzymolysis time 0.5h
In described step F1, special filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 3.4:
1.5∶2。
Peptide content the results are shown in Table 1 in active peptide in measure embodiment 1~3.
The content assaying method of peptide is：Carried out according to the assay method of peptide content in the soy peptide powders of GB/T 22492.
Table 1
| Peptide content | Embodiment 1 | Embodiment 2 | Embodiment 3 |
| Refined peony seeds activity peptide content % | 81.9 | 82.1 | 83.8 |
| Full nutrition peony seeds activity peptide content % | 32.2 | 32.8 | 33.9 |
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of preparation method of peony seeds active peptide, it is characterised in that comprise the following steps：
A, peony seeds are pre-processed, carries out ultramicro grinding, crossed 200~500 mesh, add water to mix, adjusted at 8~9 micro- alkalization of pH
Reason, soak 18~24h.Feed liquid in mechanical agitation, retort centrifuges, and collects supernatant；
B, weak acid, precipitating proteins are added in supernatant.The pH value of supernatant is adjusted to after 4~6, mechanical agitation, centrifugation, receipts
Precipitation is taken, precipitation is peony seeds albumen；
C, precipitation plus water are redissolved, heating so that protein denaturation, then carry out high-pressure homogeneous；
D, above-mentioned pH value of solution is adjusted to 5~10, adds special enzyme preparation, enzymolysis；
E, after enzymolysis terminates, specific Chinese traditional medicine composition is added, extraction enzyme deactivation obtains thick enzymolysis liquid after terminating；
F, peony seeds active peptide powder is made in thick enzymolysis liquid.
2. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that F-step includes：
F1：For thick enzymolysis liquid is centrifuged, the plate and frame filter press filtering equipped with the combination of special filtration adjuvant is then crossed；
F2：Filtrate is used into ceramic membrane filter of the aperture for 20~500nm, the active peptide permeate of clarification is made；
F3：Filtrate in step F2 is evaporated concentration, then spray drying obtains refined peony seeds active peptide powder.
3. the preparation method of peony seeds peptide living as claimed in claim 1, it is characterised in that F-step includes：
F11：By the direct rotary evaporation of thick enzymolysis liquid, emulsifying, then spray drying obtains full nutrition peony seeds active peptide powder.
4. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that described weak in described step B
Acid is acetic acid, the one or more in lactic acid, citric acid, 8~12h of time.
5. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that in described step C, heating temperature
Spend for 75~100 degree, 15~30 minutes time.
6. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that described step D, specific enzyme system
Agent combination includes flavor protease: trypsase: pepsin is 1~2: 1~2: 2~3.
7. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that described step D, enzymatic hydrolysis condition
For：It is 40~65 DEG C to extract hydrolysis temperature, and the time is 1.5~6h.
8. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that in described step E, in specific
Medicine is combined as：Mulberry leaf and perilla seed, addition are respectively the 1~2% of dry peony seeds powder solid masses.Extraction enzyme deactivation condition be：
Enzyme-removal temperature is 90~125 DEG C, and enzymolysis time is 0.5~3h.
9. the preparation method of peony seeds active peptide as claimed in claim 1, it is characterised in that special in described step F1
Filtration adjuvant is combined as：Activated carbon, perlite, diatomite, its ratio are 3~4: 1~2: 2~3.
10. a kind of preparation method of peony seeds active peptide, it is characterised in that according to any described preparation side of claim 1 to 9
Method is made, mass content >=80% of the refined peony seeds active peptide, the mass content of the full nutrition peony seeds active peptide
>=30%.
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| CN116970673A (en) * | 2023-09-11 | 2023-10-31 | 山东大树达孚特膳食品有限公司 | Anti-fatigue peony peptide and preparation method thereof |
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| CN114410724A (en) * | 2022-03-02 | 2022-04-29 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibition function and preparation method and application thereof |
| CN114410724B (en) * | 2022-03-02 | 2023-09-15 | 厦门元之道生物科技有限公司 | Paeonia ostii peptide with tyrosinase activity inhibiting function and preparation method and application thereof |
| CN116970673A (en) * | 2023-09-11 | 2023-10-31 | 山东大树达孚特膳食品有限公司 | Anti-fatigue peony peptide and preparation method thereof |
| CN116970673B (en) * | 2023-09-11 | 2024-02-23 | 山东大树达孚特膳食品有限公司 | Anti-fatigue peony peptide and preparation method thereof |
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