CN107176972A - A kind of ring shrimp shrimp head ace inhibitory peptide and preparation method thereof - Google Patents
A kind of ring shrimp shrimp head ace inhibitory peptide and preparation method thereof Download PDFInfo
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- CN107176972A CN107176972A CN201710412247.8A CN201710412247A CN107176972A CN 107176972 A CN107176972 A CN 107176972A CN 201710412247 A CN201710412247 A CN 201710412247A CN 107176972 A CN107176972 A CN 107176972A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
Abstract
The present invention relates to a kind of ring shrimp shrimp head ace inhibitory peptide, its amino acid sequence is His Ala Phe；The preparation method of ring shrimp shrimp head ace inhibitory peptide is further related to, it comprises the following steps：Pretreatment of raw material；Ultrasound-assisted enzymolysis；Ultrafiltration through membranes processing；Treatment on ion exchange columns；Gel column processing；And RPLC processing.The ring shrimp shrimp head ace inhibitory peptide of the present invention, it has stronger ACE inhibitory activity, and with ring shrimp shrimp head for raw material, solve the problems, such as the leftover bits and pieces produced in ring shrimp process, the theory significance having to the economic value for further improving ring shrimp, in addition, the present invention combines ultrasonication and protease hydrolyzed, improve percent hydrolysis, fully extract the active ingredient in raw material, improve recovery rate, and extracting mode is gentle, active ingredient to be extracted will not be damaged, isolated and purified simultaneously by multiple, Stepwise Screening, obtain the reliable ring shrimp shrimp head ace inhibitory peptide of structure.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of ring shrimp shrimp head ace inhibitory peptide and preparation method thereof.
Background technology
Ring shrimp (Penaeus japonicus) scientific name japonicus, are under the jurisdiction of Decapoda, Penaeidae, are in the world three
Cultured area and the maximum prawn culturing kind of yield in big shrimps in culture, because its is beautiful in colour and is in spot nodular, so gain the name,
There is the reputation of " after shrimp ".Not only individual is big for ring shrimp, and delicious meat, is the fine work in marine product, therefore be well suited for cooking sushi
The products such as shrimp.
At present, most of ring shrimp is processed to decaptitating frozen fresh prawn meat products, for domestic or outlet, so that processed
About 35% shrimp head leftover bits and pieces is produced in journey, wherein, shrimp head meat accounts for the 30% of shrimp head leftover bits and pieces weight.Due in shrimp head
Containing the sandstone hard constituents such as gastric pouch, mouthpart, it is impossible to directly eat, therefore, the shrimp head accessory substance in how being processed to ring shrimp enters
Row deep processing is current urgent problem to be solved to improve added value of product.Current research of the domestic and foreign scholars to ring shrimp is most
Concentrate in terms of nursery, cultivation, biology morphology and habit, such as (the Publication No. of Application No. 201410346224.8
CN104115772A Chinese invention patent)《A kind of freshwater aquiculture pond management method of ring shrimp》.
Hypertension is, to a kind of great disease of human health risk, a series of cardiovascular and cerebrovascular diseases can be induced, when serious
Death can be caused.It is to cause blood in human body in pressure elevated in angiotensin converting enzyme (ACE) generally existing mammalian tissues
One big factor.Vel-Tyr-Pro-Trp-Thr-Gln-Arg-Phe can suppress ACE activity, reach the purpose reduced blood pressure.At present, most of hyperpietics
Take synthesis Vel-Tyr-Pro-Trp-Thr-Gln-Arg-Phe to control the rise of blood pressure, synthesize the features such as Vel-Tyr-Pro-Trp-Thr-Gln-Arg-Phe has strong effect, instant effect, but to kidney
Function has certain side effect.
In recent years, the ace inhibitory peptide separated from animal and plant resource, it is secondary without poison because it does not influence on normal arterial pressure
The advantages of effect, therefore with very wide application prospect.Existing ace inhibitory peptide is mainly by protein through protease hydrolytic
Produce, the kinds of protein of use is various, predominantly land protein resource, such as soybean protein, lactalbumin, zein
Deng.Also increasingly paid close attention in addition, extracting albumen from marine organisms and preparing ace inhibitory peptide by people, such as Patent No.
ZL201210176511.X (Authorization Notice No. is CN102690854B) Chinese invention patent《One kind prepares blood vessel using shrimp shell
The method of Angiotensin Converting enzyme inhibition peptide》, Patent No. ZL200610108298.3 (Authorization Notice No. is CN1908009B)
Chinese invention patent《Chinese green food protein antihypertensive peptide and preparation method and application》Deng.Ring shrimp is utilized however, having no at present
Shrimp head prepares the research report of ace inhibitory peptide, and the ACE inhibitory activity to ring shrimp shrimp head is studied, can not only be fully sharp
With ring shrimp shrimp head leftover bits and pieces, the added value of product of ring shrimp is improved, and is had to the research for expanding ace inhibitory peptide important
Theory significance.
The content of the invention
It is strong that first technical problem to be solved by this invention provides a kind of ACE inhibitory activity for prior art
Ring shrimp shrimp head ace inhibitory peptide.
Second technical problem to be solved by this invention is to provide a kind of above-mentioned ring shrimp shrimp head for prior art
The preparation method of ace inhibitory peptide.
3rd technical problem to be solved by this invention is that a kind of above-mentioned ring shrimp of raising is provided for prior art
The method of shrimp head ace inhibitory peptide stability in vivo.
The present invention solve technical scheme that above-mentioned first technical problem used for：A kind of ring shrimp shrimp head ACE suppresses
Peptide, it is characterised in that the amino acid sequence of the ring shrimp shrimp head ace inhibitory peptide is His-Ala-Phe.
The present invention solve technical scheme that above-mentioned second technical problem used for：A kind of ring shrimp shrimp as described above
The preparation method of head ace inhibitory peptide, it is characterised in that comprise the following steps：
(1) pretreatment of raw material：Ring shrimp shrimp head is cleaned up, lipid components are removed, it is standby that homogenate is twisted into slurries；
(2) ultrasound-assisted enzymolysis：Ultra-pure water being added to above-mentioned slurries solution being made, solid-liquid ratio is 1:6~14w/v, is adjusted
PH value is saved to 6~8, bromelain is added, enzyme concentration is ultrasound-assisted enzymolysis 20 at 2000~6000U/g, 45~65 DEG C
~60min, after enzymolysis terminates, boiling water inactivation, centrifuging and taking supernatant must digest solution；
(3) hyperfiltration treatment：By above-mentioned enzymolysis solution after micro-filtration, then after ultra-filtration and separation molecular weight be more than 1kDa groups with
Molecular weight is less than two components of 1kDa groups, determines the ACE inhibitory activity of each component after freezing respectively；
(4) treatment on ion exchange columns：In each group obtained after ultrafiltration through membranes separation, ACE inhibitory activity is chosen most strong
Component, which adds water, is configured to solution, through DEAE-Sephadex ion exchange column separating purifications, collects each peak, and each group is determined after freezing
ACE inhibitory activity；
(5) gel column is handled：In each group obtained after ion exchange post separation, ACE inhibitory activity most strong group is chosen
Divide to add water and be configured to solution, after desalination, through Sephadex G-25 gel column separating purifications, collect each peak, each group is determined after freezing
ACE inhibitory activity；
(6) RPLC is handled：In each group obtained after gel post separation, ACE inhibitory activity is chosen most
Strong component, which adds water, is configured to solution, is isolated and purified after membrane filtration with RP-HPLC, collects each eluting peak, dense
Determine the ACE inhibitory activity of each group after contracting respectively, wherein ACE inhibitory activity most strong group be needed for ring shrimp shrimp head ACE
Peptide for inhibiting.
Preferably, in the step (2), supersonic frequency is 10~30kHz.
Preferably, in the step (4), eluent is ultra-pure water, flow velocity is 1.0mLmin-1。
Preferably, in the step (5), the chromatographic condition of RPLC is：Using C18Chromatographic column 4.6 ×
250mm5 μm, A phases are the acetonitrile containing 0.1% trifluoroacetic acid, and B phases are the ultra-pure water containing 0.1% trifluoroacetic acid, 25 DEG C of column temperature, stream
Fast 1.0mLmin-1, elution process is：0%~100%B elutes 8min, 15%~85%B elutions 17min, 30%~70%B
Elute 10min, 0%~100%B elutions 5min.
The present invention solve technical scheme that above-mentioned 3rd technical problem used for：In ring shrimp shrimp head ACE suppressions
Chicken egg white is added in peptide processed, and the mass ratio of ring shrimp shrimp head ace inhibitory peptide and chicken egg white is 1:4~6.
Compared with prior art, the advantage of the invention is that：The invention provides a kind of brand-new ring shrimp shrimp head ACE suppressions
Peptide processed, it has stronger ACE inhibitory activity and ACE inhibitory activity up to more than 85%, can be widely applied in antihypertensive product；
The present invention is with ring shrimp shrimp head for raw material, and source is wide, and cost is low, solves the leftover bits and pieces produced in ring shrimp process and asks
Topic, the theory significance having to the economic value for further improving ring shrimp, in addition, of the invention by ultrasonication and protease
Enzymolysis is combined, and is improved percent hydrolysis, is fully extracted the active ingredient in raw material, improves yield, and extracting mode is gentle, will not treat
The active ingredient of extraction is damaged, at the same by it is multiple isolate and purify, Stepwise Screening, obtain the reliable ring shrimp shrimp head of structure
Ace inhibitory peptide.
Brief description of the drawings
Fig. 1 is the ion exchange column collection of illustrative plates of the components of SHP- II in the embodiment of the present invention 1；
Fig. 2 be the embodiment of the present invention 1 in the components of SHP- II ionic energy transfer after each component ACE inhibiting rates；
Fig. 3 is the Sephadex G-25 tomographic maps of F5 components in the embodiment of the present invention 1；
The ACE inhibiting rates at each peak after Fig. 4 chromatographs for the Sephadex G-25 of F5 components in the embodiment of the present invention 1；
Fig. 5 is the RP-HPLC separating spectrum of F52 components in the embodiment of the present invention 1；
Fig. 6 is the RP-HPLC separating spectrum of SHP-C components in the embodiment of the present invention 1；
Fig. 7 is the polypeptide mass spectrogram of SHP-C components in the embodiment of the present invention 2.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
Embodiment 1：The preparation of ring shrimp shrimp head ace inhibitory peptide
1.1 raw materials and reagent
Raw material：Ring shrimp shrimp head, purchased from the holy biological Co., Ltd in Zhoushan sea.
Reagent：Trypsase, bromelain, pepsin, alkali protease, neutral proteinase, alkali protease with
And acid protease, above-mentioned each protease is purchased from Nanning Pang Bo bioengineering Co., Ltd；Gly、Gly-Gly-Gly、
Vitamin B12, Aprotinin, cromoci, bovine serum albumin(BSA), acetonitrile (chromatographic grade), trifluoroacetic acid (chromatographic grade), formic acid (color
Spectrum level), chromatographic column filler Sephadex G-25 (Sigma Co., USA)；Absolute ethyl alcohol, 2,6 di tert butyl 4 methyl phenol
(BHT), the reagent such as hydrochloric acid, sodium hydroxide is to analyze pure (Chemical Reagent Co., Ltd., Sinopharm Group).
1.2 instrument and equipment
WATERS SYNAPT ultrahigh pressure liquid phase chromatogram level Four bar time-of-flight mass spectrometry instrument (Waters, US)；
UPLC BEH C18 posts (2.l ID × 120mm) (Waters, US)；SunfireTMC18 posts (4.6 × 250mm) (U.S.
Waters companies)；Alliannce e2695 high performance liquid chromatographs (Waters, US)；The eggs of AKTA purifier 100
White purification system (Amersham Biosciences companies of Sweden)；HiTrapTMDesalting prepacked columns (GE companies of the U.S.)；
2.6 × 100cm chromatographic columns (buy securities with all one's capital Science and Technology Ltd. in Beijing)；PelliconTM-2Mini Holder Catalog
XX42PMINI ultrafiltration apparatus (MILLIPORE companies of the U.S.)；BT4K-ZL types freeze drier (VIRTIS companies of the U.S.)；DL-
720A ultrasonic cleaners (Shanghai Shuan Xu Electronics Co., Ltd.s)；UV-8900 ultraviolet-uisible spectrophotometers (Shanghai member analyzer device
Co., Ltd)；FJ300SH digital display high speed homogeneous dispersions machine (upper oceanic rise includes experimental instruments and equipment limited).
The extraction separation of 1.3 ring shrimp shrimps head ace inhibitory peptide
(1) pretreatment of raw material：Ring shrimp shrimp head is cleaned up, the lipid components such as shrimp cream are removed, it is even with high speed homogenizer
It is standby that slurry is twisted into slurries.
(2) ultrasound-assisted enzymolysis：Controlled in real time using continuous ultrasound assistance enzymolysis mode, and by thermostatic water-circulator bath
Ultrasonic wave added hydrolysis temperature processed, detailed process is：Ultra-pure water being added to above-mentioned slurries solution being made, solid-liquid ratio is 1:6~14w/
V, and adjust pH value to 6~8, adds 20~60min of ultrasound-assisted enzymolysis at protease, 45~65 DEG C, after enzymolysis terminates,
Boiling water bath 10min is inactivated, and centrifugation (4000r/min, 10min) takes supernatant to digest solution (being named as SHP), if on after centrifugation
Floating has a small amount of grease in clear liquid, can be absorbed with suction pipe.
(3) ultrafiltration through membranes are handled：By above-mentioned enzymolysis solution by 0.45 μm of filter membrane micro-filtration after, molecular weight be more than 1kDa groups with
Molecular weight is less than two components of 1kDa groups, determines the ACE inhibitory activity of each group after freezing respectively.The measure ginseng of ACE inhibitory activity
According to Cushman (Cushman D W, Cheung H S.Spectrophoto metric assay and prprtes of the
Angiotensin-converting enzyme of rabbit lung [J] .Biochem Pharm acol, 1971,20：
Method 637-1648) is improved.Using Hippuryl-His-Leu as substrate, determine suppression of the polypeptide ultrafiltrate to ACE and live
Property, rapidly decomposition produces hippuric acid (Hip) and dipeptides (His-Leu, HL) to polypeptide HHL under the catalysis of ACE enzymes, and hippuric acid exists
There is obtained the maximum absorption at 228nm.
(4) treatment on ion exchange columns：In each group obtained after ultrafiltration through membranes separation, ACE inhibitory activity is chosen most strong
Component adds ultra-pure water to be configured to solution, through DEAE-Sephadex ion exchange column separating purifications, collects each peak, is determined after freezing
The ACE inhibitory activity of each group；
(5) gel column is handled：In each group obtained after ion exchange post separation, ACE inhibitory activity most strong group is chosen
Divide plus ultra-pure water is configured to solution, through HiTrapTMAfter Desalting prepacked column desalinations, through Sephadex G-25 gel columns point
From purifying, each peak is collected, the ACE inhibitory activity of each group is determined after freezing, eluent is ultrapure wherein in gel column separating purification
Water, flow velocity is 1.0mLmin-1。
(6) RPLC is handled：In each group obtained after gel post separation, ACE inhibitory activity is chosen most
Strong component adds ultra-pure water to be configured to solution, is isolated and purified after membrane filtration with RP-HPLC, collects each elution
Peak, determines the ACE inhibitory activity of each group respectively after concentration, wherein ACE inhibitory activity most strong component is required ring shrimp
Ace inhibitory peptide；The chromatographic condition of RPLC is：Using C184.6 × 250mm5 μm of chromatographic column, A phases are containing 0.1%
The acetonitrile of trifluoroacetic acid, B phases are the ultra-pure water containing 0.1% trifluoroacetic acid, 25 DEG C of column temperature, flow velocity 1.0mLmin-1, elution requirement
As described in Table 1.
Table 1RP-HPLC elution requirements
| Time/min | Flow velocity/(mLmin-1) | A/% | B/% |
| 0 | 1.0 | 0 | 100 |
| 8 | 1.0 | 0 | 100 |
| 25 | 1.0 | 15 | 85 |
| 35 | 1.0 | 30 | 70 |
| 40 | 1.0 | 0 | 100 |
1.3.1 the screening of hydrolase
Select trypsase, bromelain, pepsin, alkali protease, neutral proteinase, alkali protease and
7 kinds of proteolytic enzymes of acid protease, above-mentioned 7 kinds of proteolytic enzymes digest ring shrimp head, enzyme under respective optimum condition respectively
The ACE inhibitory activity change for solving ring shrimp head is as described in Table 2.
The ACE inhibitory activity change of the different hydrolysising protease ring shrimp heads of table 2
| Protease species | Enzymatic activity (U/g) | Temperature (DEG C) | pH | Concentration of substrate (%) | ACE inhibitory activity peptide |
| Papain | 1000 | 55 | 6.5 | 10 | 35.27±0.02 |
| Neutral proteinase | 1000 | 50 | 6.5 | 10 | 52.18±0.03 |
| Bromelain | 1000 | 55 | 6.5 | 10 | 63.02±0.05 |
| Pepsin | 1000 | 40 | 2.5 | 10 | 58.31±0.04 |
| Trypsase | 1000 | 37 | 6.5 | 10 | 48.65±0.01 |
| Alkali protease | 1000 | 50 | 8.0 | 10 | 43.77±0.01 |
| Acid protease | 1000 | 40 | 3.0 | 10 | 53.44±0.06 |
From table 2, the ACE inhibitory activity of the enzymolysis liquid of acquisition of the bromelain under its optimum condition is best.It is comprehensive
Upper described, bromelain is to prepare the ideal protease of ring shrimp shrimp head ace inhibitory peptide in the present invention.
1.3.2 the determination of ultrasound condition
(1) influence of the ultrasonic temperature to ring shrimp shrimp head enzymolysis product antioxidation activity：It is 40min, surpasses in ultrasonic time
Acoustic frequency is that 20kHz, solid-liquid ratio are 1:8 (w/v), pH value are ultrasonic temperature difference under conditions of 6.5, enzyme concentration is 4000U/g
It is set as 45 DEG C, 50 DEG C, 55 DEG C, 60 DEG C, 65 DEG C.
(2) influence of the ultrasonic time to ring shrimp shrimp head enzymolysis product antioxidation activity：It is 55 DEG C, ultrasound in ultrasonic temperature
Frequency is that 20kHz, solid-liquid ratio are 1:8 (w/v), pH value are that ultrasonic time is set respectively under conditions of 6.5, enzyme concentration is 4000U/g
It is set to 20min, 30min, 40min, 50min, 60min.
(3) influence of the supersonic frequency to ring shrimp shrimp head enzymolysis product antioxidation activity：It is 55 DEG C, feed liquid in ultrasonic temperature
Than for 1:8 (w/v), ultrasonic time is that 40min, pH value are that supersonic frequency is set respectively under conditions of 6.5, enzyme concentration is 4000U/g
It is set to 10kHz, 15kHz, 20kHz, 25kHz, 30kHz,.
(4) influence of the solid-liquid ratio to ring shrimp shrimp head enzymolysis product antioxidation activity：Ultrasonic temperature be 55 DEG C, ultrasound when
Between be that 40min, supersonic frequency are that 20kHz, pH value are that under conditions of 6.5, enzyme concentration is 4000U/g, solid-liquid ratio is set respectively
For 1:6、1:8、1:10、1:12、1:14(w/v).
(5) influence of the pH value to ring shrimp shrimp head enzymolysis product antioxidation activity：It is 55 DEG C, ultrasonic time in ultrasonic temperature
It is that 20kHz, solid-liquid ratio are 1 for 40min, supersonic frequency:8 (w/v), enzyme concentration be 4000U/g under conditions of, pH value is set respectively
It is set to 6.00,6.50,7.00,7.50,8.00.
(6) influence of the enzyme concentration to ring shrimp shrimp head enzymolysis product antioxidation activity：Ultrasonic temperature be 55 DEG C, ultrasound when
Between be that 40min, supersonic frequency are that 20kHz, solid-liquid ratio are 1:8 (w/v), pH value be 6.5 under conditions of, enzyme concentration is set respectively
For 2000,3000,4000,5000,6000U/g.Every group of Optimal Experimental carries out three parallel laboratory tests, finally averages.
Influence of the ultrasonic temperature to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis is as shown in table 3, by table 3
Understand, ultrasonic temperature has large effect to ACE inhibitory activity and degree of hydrolysis.When ultrasonic temperature is less than 55 DEG C, enzymolysis product
Degree of hydrolysis increase with the rise of temperature, during to 55 DEG C, its degree of hydrolysis reaches peak (54.05%), during more than 55 DEG C,
Degree of hydrolysis is on a declining curve with the rise of temperature.When ultrasonic temperature be 55,60 DEG C when, DPPH clearance rates are respectively 61.26 ±
0.50%th, 58.00 ± 0.52%, there was no significant difference for both.Because appropriate heating can promote the vigor of enzyme, make water
Solution is more abundant, but too high temperature then can inhibitory enzyme activity.Consider, 55 DEG C of present invention selection is suitable ultrasound temperature
Degree.
Influence of the ultrasonic temperature of table 3 to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| T/ DEG C of ultrasonic temperature | ACE inhibiting rates/% | Degree of hydrolysis/% |
| 45 | 34.85±1.34 | 35.75±0.23 |
| 50 | 45.62±0.72 | 43.51±1.13 |
| 55 | 61.26±0.50 | 54.05±1.50 |
| 60 | 58.00±0.52 | 37.50±1.52 |
| 65 | 35.45±1.10 | 24.04±0.99 |
Influence of the ultrasonic time to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis is as described in Table 4, by table 4
Understand, ultrasonic time for 20~60min time range in, shrimp head enzymolysis product ACE inhibitory activity in first rise after under
The trend of drop.Because the time it is too short enzyme digestion reaction can be caused not abundant enough, and overlong time enzymolysis product is then easily converted
For other accessory substances, its yield is influenceed.When ultrasonic time is 40min, peak is reached, ACE inhibitory activity is 68.72 ±
0.61%.Therefore, present invention selection 40min is optimal ultrasonic time.
Influence of the ultrasonic time of table 4 to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| Ultrasonic time (min) | Degree of hydrolysis/% | ACE inhibiting rates/% |
| 20 | 34.32±0.89 | 35.09±0.08 |
| 30 | 41.24±1.10 | 45.46±1.06 |
| 40 | 51.28±1.05 | 68.72±0.61 |
| 50 | 33.64±0.49 | 62.25±0.42 |
| 60 | 31.86±0.70 | 44.03±0.77 |
Influence of the supersonic frequency to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis is as described in Table 5, by table 5
Understand, with the increase of supersonic frequency, the ACE inhibitory activity of ring shrimp shrimp head enzymolysis product is in first to raise to reduce trend afterwards, when
When supersonic frequency is 20kHz, ACE inhibitory activity highest is 64.13 ± 0.071%.Because with the increasing of supersonic frequency
Plus, enzyme and substrate collision frequency are consequently increased, and finally accelerate enzyme digestion reaction speed, if but supersonic frequency it is excessive when, enzyme
Conformation can be changed, and enzyme activity is reduced on the contrary, have impact on hydrolysis result.Therefore, present invention selection 20kHz is optimal supersonic frequency
Rate.
Influence of the supersonic frequency of table 5 to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| Supersonic frequency/KHZ | Degree of hydrolysis/% | ACE inhibiting rates/% |
| 10 | 32.90±0.62 | 53.57±0.05 |
| 15 | 45.86±1.23 | 56.37±0.21 |
| 20 | 55.15±1.35 | 64.13±0.07 |
| 25 | 51.41±2.57 | 57.05±0.09 |
| 30 | 50.11±1.30 | 55.17±0.78 |
Influence of the solid-liquid ratio to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis as described in Table 6, can by table 6
Know, when solid-liquid ratio is less than 1:When 10 (w/v), ring shrimp shrimp head enzymolysis product ACE inhibitory activity with solid-liquid ratio increase not
Disconnected increase, when solid-liquid ratio is more than 1:When 10 (w/v), DPPH clearance rates then constantly reduce.When solid-liquid ratio is 1:6~1:8 (w/v's)
In the range of when, shrimp head enzymolysis product degree of hydrolysis it is in rising trend, when solid-liquid ratio be more than 1:When 8 (w/v), then degree of hydrolysis is hardly
It is further added by.Generally, solid-liquid ratio is excessive or too small can all influence the diffusion of enzymatic speed and product molecule.Synthesis is examined
Consider, the present invention chooses solid-liquid ratio 1:10 (w/v) conveniently, and when solid-liquid ratio be 1:When 10 (w/v), ACE inhibitory activity is
66.66 ± 0.57%, degree of hydrolysis is 55.67%.
Influence of the solid-liquid ratio of table 6 to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| Solid-liquid ratio | ACE inhibiting rates/% | Degree of hydrolysis/% |
| 1:6 | 31.36±0.07 | 20.54±0.26 |
| 1:8 | 38.37±1.10 | 55.04±0.91 |
| 1:10 | 66.66±0.57 | 55.72±0.38 |
| 1:12 | 57.29±0.56 | 57.29±0.86 |
| 1:14 | 42.65±0.24 | 55.78±1.65 |
Influence of the pH value to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis is as described in Table 7, as shown in Table 7,
When pH value is less than 6.50, the ACE inhibitory activity and degree of hydrolysis of ring shrimp shrimp head enzymolysis product increase as pH value increases, when
When pH value is more than 6.50, the ACE inhibitory activity and degree of hydrolysis of ring shrimp shrimp head enzymolysis product are reduced as pH value increases.It is comprehensive
Considering, it is 6.50 more suitable that the present invention, which chooses pH value, and when pH value is 6.50, ACE inhibiting rates are 49.52 ±
0.67%, degree of hydrolysis is 23.32 ± 1.14%.
Influence of the table 7pH values to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| PH | ACE inhibiting rates/% | Degree of hydrolysis/% |
| 6.00 | 45.07±0.43 | 11.12±1.56 |
| 6.50 | 49.52±0.67 | 23.32±1.14 |
| 7.00 | 46.98±0.14 | 17.20±0.40 |
| 7.50 | 39.63±0.27 | 12.47±1.01 |
| 8.00 | 27.71±0.33 | 10.19±0.98 |
Influence of the enzyme concentration to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis as described in Table 8, can by table 8
Know, when enzyme concentration is less than 4000U/g, the ACE inhibitory activity and degree of hydrolysis of ring shrimp shrimp head enzymolysis product increase with enzyme concentration
Plus and increase, when enzyme concentration is more than 4000U/g, the ACE inhibitory activity and degree of hydrolysis of ring shrimp shrimp head enzymolysis product are with adding
Enzyme amount increases and reduced.Consider, it is that 4000U/g is more suitable that the present invention, which chooses enzyme concentration, and when enzyme concentration is
During 4000U/g, ACE inhibiting rates are 46.62 ± 1.09%, and degree of hydrolysis is 23.99 ± 0.51%.
Influence of the enzyme concentration of table 8 to ring shrimp shrimp head enzymolysis product ACE inhibitory activity and degree of hydrolysis
| Enzyme concentration/U/g | ACE inhibiting rates/% | Degree of hydrolysis/% |
| 2000 | 37.16±0.58 | 10.52±0.66 |
| 3000 | 42.09±0.55 | 13.30±0.64 |
| 4000 | 46.62±1.09 | 23.99±0.51 |
| 5000 | 41.86±0.28 | 17.91±0.75 |
| 6000 | 42.70±0.46 | 14.68±0.58 |
It can to sum up obtain, optimum enzymolysis condition is：55 DEG C of ultrasonic temperature, ultrasonic time 40min, supersonic frequency 20kHz, feed liquid
Than 1:10 (w/v), pH value is 6.5.
Enzymolysis is produced however, the fuel factor produced in fuel factor, especially ultrasonic procedure can be produced in actual tests operation
The activity of thing has considerable influence, and reason is probably that, as temperature is raised, molecule activity is strengthened, and chemical bond is unstable, and polypeptide is easy
Dehydrogenation and can not be combined with free radical, so as to reduce the antioxidation activity of enzymolysis product.Specifically for the purpose of the present invention, by follow-up
Embodiment 2 understands that the amino acid sequence of the ring shrimp shrimp head ace inhibitory peptide in the present invention is His-Ala-Phe, with molecule heat
Motion is strengthened, and arginic C=NH structures generation isomerization is changed into C-NH2 in polypeptide, with alanine in another polypeptide
Carbonyl two intra-molecular cyclic hydrogen bonds of formation, add the hydrophobicity of phenyl ring in phenylalanine so that peptide molecule space structure increases
Greatly, difficulty is combined with ACE, therefore ACE inhibitory action is reduced.
Therefore, ultrasound-assisted enzymolysis condition is adjusted to by the present invention on the basis of above-mentioned optimum process condition：Ultrasound
Temperature is 52 DEG C, and ultrasonic time is 35min, and solid-liquid ratio is 1:9, the adjustment of ultrasonic temperature and ultrasonic time can be to a certain extent
Influence of the fuel factor to polypeptide is reduced, so as to be further ensured that the ACE inhibitory activity of enzymolysis product, its bioactivity is prevented effectively from
Reduction, so as to ensure its biological value.
1.3.3 hyperfiltration treatment
With 55 DEG C of ultrasonic temperature, ultrasonic time 40min, supersonic frequency 20kHz, solid-liquid ratio 1:10 (w/v), pH value is 6.5,
Enzyme concentration prepares enzymolysis product SHP for 4000U/g optimal conditions, and initial gross separation is carried out to SHP using ultra-filtration and separation equipment, point
SHP- I is not obtained (to be more than<1kDa), SHP- II (is less than<1kDa) 2 components, determine the ACE of different relative molecular masses respectively
Inhibitory activity, as a result shows, the relative molecular mass size of enzymolysis product has a significant impact to its ACE inhibitory activity tool.Its
In, SHP- II (is less than<1kDa) ACE inhibitory activity of component is higher (as shown in table 9).Therefore, SHP- II is defined as into one
Walk research object.
The ACE inhibitory activity of each component after the processing of the ultrafiltration through membranes of table 9
1.3.4 treatment on ion exchange columns
The above-mentioned components of SHP- II are further subjected to ion exchange post separation, selection is that DEAE-Sephadex ions are handed over
Post (1.6 × 70cm) is changed, sample size is 5ml, and sample concentration is 30mg/mL.The components of SHP- II are washed through ion exchange post separation, salt
Five components (as shown in Figure 1) of F1, F2, F3, F4, F5 are obtained after de-.ACE suppression of each component under various concentrations is determined respectively
System activity, as shown in Figure 2, component F5 ACE inhibitory activity is most strong.
1.3.5 gel column is handled
The F5 components that ultra-filtration and separation is obtained are by Sephadex G-25 gel chromatography post separations, using ultra-pure water as elution
Liquid, sample concentration is 20mgmL-1, applied sample amount is 5.0mL, and flow velocity is 1.0mLmin-1, collected, often managed with automatic collector
2.0mL.The collection liquid at same peak is merged, it is freeze-dried after determine the ACE inhibiting rates at each peak respectively.
From the figure 3, it may be seen that after the separation of Sephadex G-25 gel chromatographies, sample isolated F51, F52, F53, F54
Four components, component F51 is eluted out at first, represents component polypeptides molecular weight maximum, and component F54 is finally eluted out,
Molecular weight minimum is represented, component F52 peak area is maximum, illustrates component polypeptides content highest in 4 components.Such as Fig. 4 institutes
Show, be 1.0mgmL in content of peptides-1When, the DPPH clearance rates of 4 components are respectively 32.51% ± 3.45%, 46.04%
± 2.61%, 37.29% ± 1.67%, 41.61% ± 3.71%, wherein, component F52 ACE inhibiting rate highests show the group
Divide and contain more polypeptides with anti-ACE.By the ACE inhibitory activity of the component compared with ultrafiltrate, ACE inhibiting rates are improved
31.4%, illustrate that Sephadex G-25 gel chromatographies have reached the effect being further purified.
1.3.6 RPLC is handled
Separation is further purified with RP-HPLC by the isolated active component F52 of Sephadex G-25 gel chromatographies,
Obtain the main peptide peaks (as shown in Figure 5) of SHP-A, SHP-B, SHP-C, SHP-D 4.4 peaks are collected respectively, with ACE after freezing
Inhibiting rate is its ACE inhibitory activity of index determining, is 1.5mgmL in content of peptides-1When, the ACE inhibiting rates difference of 4 components
For 52.36 ± 0.12%, 68.45 ± 0.27%, 88.19 ± 0.57%, 70.27 ± 0.24%, component SHP-C has stronger
ACE inhibitory activity.Collect the component be used for carry out purity detecting analysis, with the acetonitrile containing 0.1% trifluoroacetic acid/contain 0.1% 3
The ultra-pure water of fluoroacetic acid is mobile phase, flow velocity 1.0mLmin-1, elution mode is the acetonitrile containing 0.1% trifluoroacetic acid in 20min
Interior concentration changes to 20% linear elution pattern by 0, it can be seen from testing result, and the component has preferable separating degree (such as Fig. 6
It is shown).
Embodiment 2：Ace inhibitory peptide Structural Identification
ACE constituents for suppressing SHP-C after inverted efficient liquid phase is isolated and purified is carried out using UPLC-TOF-MS/MS technologies
Structural Identification, and its structure is analyzed using peptide sequencing in Masslynx softwares, as a result show, from
SHP-C components identify peptide sequence of 1 reliability more than 90%, and its structure is His-Ala-Phe (as shown in Figure 7).
Embodiment 3：Ring shrimp shrimp head ace inhibitory peptide biological stability is determined
Prepare simulated gastric fluid and simulated intestinal fluid is standby, the process for preparation of wherein simulated gastric fluid is as follows：Dilute salt is measured respectively
Sour 16.4mL, distilled water 800mL, they are mixed, and add pepsin 10g, and concussion is uniform, is placed in volumetric flask,
And it is settled to 1000mL.The preparation of simulated intestinal fluid：6.8g potassium dihydrogen phosphate is weighed, is dissolved in 500mL distilled water,
PH value is adjusted to 6.8 with 0.1mol/L NaOH solution, and takes 10g trypsase, is dissolved in a certain amount of distilled water
In, concussion is uniform, and constant volume is to 1000mL.
The chicken egg white solution and ring shrimp shrimp head ace inhibitory peptide solution of same concentrations (10mg/ml) are prepared, with (1:
4、1:5、1:6) ratio mixing, adds the standby simulated gastric fluid or intestinal juice that prepare of equivalent, adds chicken egg white solution
3h is stood in 37 DEG C of water-bath with the ring shrimp shrimp head ace inhibitory peptide solution of simulated gastric fluid (intestinal juice), then in boiling water bath
15min, is that 5000r/min, temperature are under conditions of -4 DEG C after centrifugation 10min, and to determine its DPPH radicals scavenging in rotating speed
Rate and ABTS free radical scavenging activities, repeat experiment 3 times, chicken egg white is to ring shrimp shrimp head ace inhibitory peptide ACE inhibitory activity
Influence is as shown in table 10.
The ring shrimp shrimp of table 10 head ace inhibitory peptide ACE inhibitory activity conservation rate/%
| The ratio of chicken egg white and ring shrimp shrimp head ace inhibitory peptide solution | Pepsin | Trypsase |
| It is not added with chicken egg white solution | 81.12±1.01 | 65.51±0.81 |
| 1:4 | 92.59±0.47 | 67.83±0.11 |
| 1:5 | 94.19±0.66 | 73.12±1.34 |
| 1:6 | 87.60±1.14 | 65.33±0.79 |
From above-mentioned table 10, addition chicken egg white is in the environment of simulate the gastric juice and intestinal juice to ring shrimp shrimp head ACE
Peptide for inhibiting has preferable protective effect, and is compared with simulated intestinal fluid environment, and chicken egg white is right under simulate the gastric juice environment
The bioactivity of ring shrimp shrimp head ace inhibitory peptide has more preferable protective effect.
Claims (6)
1. a kind of ring shrimp shrimp head ace inhibitory peptide, it is characterised in that the amino acid sequence of the ring shrimp shrimp head ace inhibitory peptide is
His-Ala-Phe。
2. a kind of preparation method of ring shrimp shrimp head ace inhibitory peptide as claimed in claim 1, it is characterised in that including following step
Suddenly：
(1) pretreatment of raw material：Ring shrimp shrimp head is cleaned up, lipid components are removed, it is standby that homogenate is twisted into slurries；
(2) ultrasound-assisted enzymolysis：Ultra-pure water being added to above-mentioned slurries solution being made, solid-liquid ratio is 1:6~14w/v, adjusts pH
Value adds bromelain to 6~8, enzyme concentration be ultrasound-assisted enzymolysis 20 at 2000~6000U/g, 45~65 DEG C~
60min, after enzymolysis terminates, boiling water inactivation, centrifuging and taking supernatant must digest solution；
(3) hyperfiltration treatment：By above-mentioned enzymolysis solution after micro-filtration, molecular weight is then obtained after ultra-filtration and separation and is more than 1kDa groups and molecule
Amount determines the ACE inhibitory activity of each component respectively less than two components of 1kDa groups after freezing；
(4) treatment on ion exchange columns：After ultrafiltration through membranes separation in each group that obtains, ACE inhibitory activity most strong group group is chosen
Divide plus ultra-pure water is configured to solution, through DEAE-Sephadex ion exchange column separating purifications, collect each peak, determined respectively after freezing
The ACE inhibitory activity of group；
(5) gel column is handled：In each group obtained after ion exchange post separation, choose ACE inhibitory activity most strong component and add
Ultra-pure water is configured to solution, after desalination, through Sephadex G-25 gel column separating purifications, collects each peak, and each group is determined after freezing
ACE inhibitory activity；
(6) RPLC is handled：In each group obtained after gel post separation, ACE inhibitory activity is chosen most strong
Component, which adds water, is configured to solution, is isolated and purified after membrane filtration with RP-HPLC, each eluting peak is collected, after concentration
The ACE inhibitory activity of each group is determined respectively, and the ring shrimp shrimp head ACE needed for wherein ACE inhibitory activity most strong group is suppresses
Peptide.
3. preparation method as claimed in claim 2, it is characterised in that in the step (2), supersonic frequency is 10~30kHz.
4. preparation method as claimed in claim 2, it is characterised in that in the step (4), eluent is ultra-pure water, flow velocity
For 1.0mLmin-1。
5. preparation method as claimed in claim 2, it is characterised in that in the step (5), the color of RPLC
Spectral condition is：Using C184.6 × 250mm5 μm of chromatographic column, A phases are the acetonitrile containing 0.1% trifluoroacetic acid, and B phases are containing 0.1% 3
The ultra-pure water of fluoroacetic acid, 25 DEG C of column temperature, flow velocity 1.0mLmin-1, elution process is：0%~100%B elute 8min, 15%~
85%B elutes 17min, 30%~70%B elution 10min, 0%~100%B elutions 5min.
6. a kind of method for improving ring shrimp shrimp as claimed in claim 1 head ace inhibitory peptide stability in vivo, it is special
Levy and be to comprise the following steps, ring shrimp shrimp head ace inhibitory peptide and chicken egg white are each configured to the molten of same concentration
Liquid, chicken egg white solution, and chicken egg white solution and the bamboo are added in the ring shrimp shrimp head ace inhibitory peptide solution of configuration
The volume ratio for saving shrimp shrimp head ace inhibitory peptide solution is 1:4~6.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085357A (en) * | 2017-12-28 | 2018-05-29 | 澳优乳业（中国）有限公司 | A kind of sheep breast lactalbumin immunomodulatory peptides and its compound and its application |
| CN112501230A (en) * | 2020-12-17 | 2021-03-16 | 浙江海洋大学 | Preparation method and application of urechis unicinctus ACE inhibitory peptide |
| CN112852909A (en) * | 2021-01-25 | 2021-05-28 | 韩山师范学院 | Method for preparing ACE inhibitory peptide by solid-state fermentation of shrimp heads with mixed strains |
| CN113293188A (en) * | 2020-02-24 | 2021-08-24 | 集美大学 | Method for enzymolysis of soy sauce residue protein by using shrimp head enzyme |
-
2017
- 2017-06-05 CN CN201710412247.8A patent/CN107176972A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| BRADLEY R. HART等: "Synthetic Peptide Receptors: Molecularly Imprinted Polymers for the Recognition of Peptides Using Peptide-Metal Interactions", 《J. AM. CHEM. SOC.》 * |
| 冯屏 等: "虾头蛋白制降血压肽酶解条件及分子量分布研究", 《食品科技》 * |
| 徐洋 等: "超声辅助酶法回收南极磷虾壳中蛋白质的研究", 《安徽农业科学》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108085357A (en) * | 2017-12-28 | 2018-05-29 | 澳优乳业（中国）有限公司 | A kind of sheep breast lactalbumin immunomodulatory peptides and its compound and its application |
| CN113293188A (en) * | 2020-02-24 | 2021-08-24 | 集美大学 | Method for enzymolysis of soy sauce residue protein by using shrimp head enzyme |
| CN112501230A (en) * | 2020-12-17 | 2021-03-16 | 浙江海洋大学 | Preparation method and application of urechis unicinctus ACE inhibitory peptide |
| CN112852909A (en) * | 2021-01-25 | 2021-05-28 | 韩山师范学院 | Method for preparing ACE inhibitory peptide by solid-state fermentation of shrimp heads with mixed strains |
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