CN107176973A - A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland - Google Patents

A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland Download PDF

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CN107176973A
CN107176973A CN201710160381.3A CN201710160381A CN107176973A CN 107176973 A CN107176973 A CN 107176973A CN 201710160381 A CN201710160381 A CN 201710160381A CN 107176973 A CN107176973 A CN 107176973A
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oxidant
enzymolysis
parts
north pacific
squid
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CN107176973B (en
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杨最素
刘倩茹
柏圣达
余方苗
丁国芳
唐云平
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Zhejiang Ocean University ZJOU
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/29—Berberidaceae (Barberry family), e.g. barberry, cohosh or mayapple
    • A61K36/296—Epimedium
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/481—Astragalus (milkvetch)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537—Salvia (sage)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/74—Rubiaceae (Madder family)
    • A61K36/748—Oldenlandia or Hedyotis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
    • A61K38/08—Peptides having 5 to 11 amino acids

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Abstract

The invention discloses a kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland.The present invention is purified by ultrafiltration, HPLC and North Pacific's squid spawn tangled gland anti-oxidant enzymolysis oligopeptide is made using North Pacific's squid spawn tangled gland as raw material, and the amino acid sequence of the oligopeptides is Ala Tyr Ala Ser Ser, and molecular weight is 497.50Da, and molecular structure is,。

Description

A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland
Technical field
The present invention relates to a kind of marine biomaterial, and in particular to a kind of North Pacific squid spawn tangled gland antioxidase Solve oligopeptides.
Background technology
The intermediate product that body is produced in metabolic processes such as superoxide anion（O2-）、H2O2, peroxidating from By base（ROO）And hydroxy radical（-OH）With very strong oxidation.Under normal physiological conditions, internal antioxidase and non- The enzyme factor can effectively remove free radical, so as to maintain the poised state of free radical.But in pathological conditions, can be rapid The peroxidating of lipid is caused to cause the damage of cell membrane, DNA and protein active, so as to cause a variety of human diseases related.Therefore, It is an important channel for defending organism disease to occur to the removing of interior free yl.Because the antioxidant majority of synthesis is present Potential safety hazard, therefore, screening is efficient, low toxicity antioxidant turns into the new trend of biology, medical science and food scientific research. There are some researches show the active peptide from multiple protein all has antioxidation activity, and enzyme solution is that biology is obtained from albumen One of effective ways of active peptide, have been widely used as a kind of method for improving protein active function and nutritive value. Protein among diet is mainly absorbed in enteron aisle in the form of polypeptide and amino acid, and polypeptide is in body antioxidation process Play an important role.In recent years to oceanic biological active peptides, the research of particularly antioxidation active peptides has made great progress. But marine active peptide not yet forms industrial scale, therefore, the enzyme engineering technology of land active peptide exploitation is used for reference, with marine organisms egg Bai Ziyuan is raw material, passes through the comprehensive study to techniques such as enzymolysis, preparations, you can develop land protein sources and chemical synthesis institute Series natural, the biologically active peptide efficiently, novel that can not be produced.
Squid is ocean cephalopod important in the world, and its growth cycle is short, fertility is strong and resource recovery is fast Speed, is a kind of sustainable marine fishery resources.In recent years, with the development of domestic and international deep sea fishing technology, squid has turned into The main marine fishing of China and aquatic products processing kind.At present, the annual squid processing capacity of China is up to 40 ~ 500,000 t, occupies the world One, processing variety is mainly North Pacific squid, Argentinian squid and Peru squid.Squid is because of the fine and smooth nutrition of its meat, wind Delicious, a variety of necessary amino acid with high protein and low fat, rich in needed by human body, enjoys consumers.In squid Typically its trunk is processed in process, the accessory substances, wherein gonad such as 50% or so internal organ is produced（Spawn tangled gland, Spermary, ovary etc.）The 20% of internal organ is accounted for, the accessory substance such as these internal organ is usually processed into feed, is used as the bait of fish；Squid liver Dirty be extracted after Squid Oil is buried；Obtained from squid spawn tangled gland generally only to make culinary art edible, biological added value it is low and not and When processing can certain pollution be caused to environment.Extraction on squid spawn tangled gland enzymolysis polypeptide has not yet to see report.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of North Pacific's squid spawn tangled gland with anti-oxidation efficacy Anti-oxidant enzymolysis oligopeptide.
The present invention is that the technical scheme that solution above-mentioned technical problem is taken is：
A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, the molecular structure of the enzymolysis oligopeptide is：
,
I.e. the amino acid sequence of the enzymolysis oligopeptide is Ala-Tyr-Ala-Ser-Ser, and molecular mass is 497.50 Da.
The application preparation of the anti-oxidant enzymolysis oligopeptide of North Pacific's squid spawn tangled gland, by weight containing such as claim 1 Described anti-oxidant enzymolysis oligopeptide 0.01-0.5 parts of North Pacific's squid spawn tangled gland, 1-3 parts of barrenwort, 1-5 parts of the Radix Astragali, Ramulus Taxilli 1-10 parts, 10-20 parts of the red sage root, 1-10 parts of radish seed, 2-10 parts of Oldenlandia diffusa Roxb, 3-20 parts of the root of kudzu vine.
Compared with prior art, the preparation for a kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland that the present invention is provided The advantage of method is：Present invention process is scientific and reasonable, simple to operate, and its lytic activity is high, and product yield is stable.Meanwhile, this hair Bright prepared North Pacific's anti-oxidant enzymolysis oligopeptide of squid spawn tangled gland is that natural material is made through enzymolysis, safe and nontoxic secondary work With significantly, squid spawn tangled gland anti-oxidant enzymolysis oligopeptide in North Pacific's can be used as antioxidant drug, functional food to antioxidation activity Further researched and developed.
Brief description of the drawings
Fig. 1 is the anti-oxidant enzymolysis oligopeptide amino acid molecular structure formula of the embodiment of the present invention 1；
Fig. 2 is absorbance of the ultrafiltration component 3-5 KD molecules section of the embodiment of the present invention 1 at 280 nm（Absorbance-pipe number）；
Fig. 3 is the DPPH clearance rates at different peaks in Fig. 2 of the present invention；
Fig. 4 is the superoxide anion clearance rate of the enzymolysis polypeptide different molecular section of the embodiment of the present invention 1；
Fig. 5 is clearance rate of the enzymolysis polypeptide different molecular section of the embodiment of the present invention 1 to DPPH；
Fig. 6 is the reducing power of the enzymolysis polypeptide different molecular section of the embodiment of the present invention 1；
Fig. 7 is the Scavenging action to hydroxyl free radical of the enzymolysis polypeptide different molecular section of the embodiment of the present invention 1；
Fig. 8 is the peak I of the embodiment of the present invention 1 high-efficient liquid phase chromatogram at 280 nm.
Embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1：
A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, the molecular structure of the enzymolysis oligopeptide is：
,
I.e. the amino acid sequence of the enzymolysis oligopeptide is Ala-Tyr-Ala-Ser-Ser, and molecular mass is 497.50 Da.
The application preparation of the anti-oxidant enzymolysis oligopeptide of North Pacific's squid spawn tangled gland, by weight containing such as claim 1 Described anti-oxidant enzymolysis oligopeptide 0.01-0.5 parts of North Pacific's squid spawn tangled gland, 1-3 parts of barrenwort, 1-5 parts of the Radix Astragali, Ramulus Taxilli 1-10 parts, 10-20 parts of the red sage root, 1-10 parts of radish seed, 2-10 parts of Oldenlandia diffusa Roxb, 3-20 parts of the root of kudzu vine.
The preparation method of the anti-oxidant enzymolysis oligopeptide of North Pacific's squid spawn tangled gland, with following steps：
1）North Pacific's squid spawn tangled gland is taken to smash homogenate to pieces, with organized enzyme at peak enzymolysis-ability temperature and pH value condition, plus albumen Enzyme, insulation are digested；Then inactivate, 0 ~ 4 oC, centrifuge under conditions of 6000 ~ 12000 r/min, take supernatant；
2）Supernatant is taken, ultrafiltration molecular cut off is that the ultrafiltration component of 3-5 KD molecules section is stand-by；
3）Take described ultrafiltration component to carry out agarose gel chromatography separation, collect first occurred at 280 nm in three peaks The component at peak；
4）The component at first described peak is taken to cross the HPLC further isolated anti-oxidant enzymolysis of North Pacific squid spawn tangled gland Oligopeptides, its molecular structure is：
。
Above-mentioned organized enzyme is alkali protease, it is preferred that hydrolysis temperature be located at 40~50 DEG C between, hydrolysis time 5~ Between 7h, pH between 8.0~10.0, enzyme concentration be located at 2500~3500U/g, solid-liquid ratio be located at 1：3~1：5.
It is preferred that the condition of enzymolysis be：Temperature 50 C, the h of enzymolysis time 7, U/g, pH value 9.0 of enzyme concentration 3500, material Liquor ratio is 1：6 be optimum enzymolysis condition
Step 3）The condition of described elution is：Column dimension：10×300-310 mm；Post material：Agarose；Post material granular size： 10 ± 2 µm；Applied sample amount：500 µL；Mobile phase：Ultra-pure water；Eluent flow rate：0.5 mL/min；Detection wavelength：280nm； Automatic collected volume：3.2 mL/ are managed.
A kind of purposes of the anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, the amino acid sequence of the enzymolysis oligopeptide is Ala-Tyr-Ala-Ser-Ser, molecular mass is that the molecular structural formula of 497.50 Da, the i.e. enzymolysis oligopeptide is：
,
Enzymolysis oligopeptide is used for the purposes for preparing antioxidant functional food or food additives.Contain North Pacific's squid spawn tangled gland Anti-oxidant enzymolysis oligopeptide 0.01-0.5 parts, 1-3 parts of barrenwort, 1-5 parts of the Radix Astragali, 1-10 parts of Ramulus Taxilli, 10-20 parts of the red sage root, radish Sub- 1-10 parts, 1-10 parts of Radix Angelicae Sinensis, the preparation of 3-20 parts of the root of kudzu vine, the purposes for food additives.
Embodiment 2：
Materials and methods
Material to be tested
North Pacific's squid spawn tangled gland is purchased in local aquatic product enterprise；Alkali protease is purchased in Asia-Pacific perseverance letter biotechnology（North Capital）Co., Ltd；DPPH is purchased in West Asia reagent；Luxuriant and rich with fragrance Lip river piperazine, Phen, ferric trichloride, disodium hydrogen phosphate are purchased in me Fourth Reagent Company；Remaining reagent is that analysis is pure, is purchased in Chemical Reagent Co., Ltd., Sinopharm Group.
Key instrument
SSW-420-2S thermostat water baths, Shanghai Min Yi Electronics Co., Ltd.s；BSA124S type electronic balances, Germany SartoriusAG companies；DS-1 tissue mashing machines, Shanghai Sample Model Factory；PHS-250PH is counted, Lida Instrument Factory, Shanghai； The type high speed low temperature centrifugal machines of CF16RX II, HIT；The UV-R type ultrapure water systems of Direct-Q 5, the U.S. Millipore companies；Cogent u Scale type ultrafiltration systems, Millipore companies of the U.S.；ALPHA 1-4/LDplus types are cold Lyophilizer, Beijing development in science and technology Co., Ltd of Song Yuan Huaxing；Ultraviolet specrophotometer（752FC）, Shanghai spectral instrument is limited Company；Agilent-1260 type high performance liquid chromatographs, Agilent company of the U.S..
Method
Experiment of single factor
Proteolysis assay is carried out from alkali protease, it is considered to hydrolysis temperature, enzymolysis time, enzyme concentration, pH value of solution and solid-liquid ratio. Hydrolysis process is as follows:
Squid spawn tangled gland is smashed → is homogenized → 1 mol/L sodium hydroxides to pieces and adjusts pH value → insulation → being hydrolyzed after enzyme-added → to go out enzyme (90 DEG C 15 min of heating) → centrifugation (10000 r/min, 15 min) → supernatant constant volume → amino nitrogen content (AAN) determines.
Between temperature is located at 40~50 DEG C, hydrolysis time between 5~7h, pH is between 8.0~10.0, enzyme concentration It is located at 1 positioned at 2500~3500U/g, solid-liquid ratio：3~1：Preferably, obtained free ANN amounts at most, are hydrolyzed hydrolysis effect when 5 Degree is higher.However, temperature 50 C, the h of enzymolysis time 7, U/g, pH value 9.0 of enzyme concentration 3500, solid-liquid ratio are 1：6 is most Good enzymatic hydrolysis condition.
Sephadex G-25 isolate and purify result
Take the lyophilized sample of 3-5 KD molecules section to carry out Ago-Gel Sephadex G-25 chromatographies, occur three at 280 nm Peak, i.e. peak I, peak II and peak III, detect that it, to DPPH clearance rates, obtains appearance I after three peak components of collection are freeze-dried respectively There is preferable Scavenging activity, see Fig. 3, take the molecule section of peak I to carry out HPLC purifying.
HPLC separation, purity detecting and subject peptide sequence measurement result
Peak I prepares result as shown in figure 8, when retention time is about 18min, there is simple spike, peak height is through HPLC chromatogram post 161.2, through amino acid sequence instrument N-terminal Sequence Detection, its amino acid sequence is Ala-Tyr-Ala-Ser-Ser, average molecular matter Measure as 497.50 Da, its structural formula is as follows：
After being chromatographed through Ago-Gel G-25, three peaks are drawn, according to the measure to DPPH clearance rates, the effect of appearance I are obtained obvious Really, DPPH clearance rate is isolated and purified by HPLC up to 61.8% and through amino acid sequence instrument N-terminal Sequence Detection, obtains target The amino acid sequence of peptide is Ala-Tyr-Ala-Ser-Ser.North Pacific's squid spawn tangled gland can obtain anti-oxidant work by enzymolysis Property preferable oligopeptides, can develop the intensive processing of squid byproduct, improve biological added value and have great importance.
Finally, in addition it is also necessary to it is noted that listed above is only a specific embodiment of the invention.Obviously, it is of the invention Above example is not limited to, there can also be many deformations.One of ordinary skill in the art can be straight from present disclosure Export or all deformations associated are connect, protection scope of the present invention is considered as.
SEQUENCE LISTING
<110>Zhejiang Ocean university
<120>A kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland
<130> zjou-yzs-20161201
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 5
<212> PRT
<213>It is artificial synthesized
<400> 1
Ala Tyr Ala Ser Ser
1 5

Claims (3)

1. a kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, it is characterised in that：The molecular structure of the enzymolysis oligopeptide is：
。
2. a kind of anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, it is characterised in that：The amino acid sequence of the enzymolysis oligopeptide For Ala-Tyr-Ala-Ser-Ser, molecular mass is 497.50 Da.
3. a kind of preparation of the anti-oxidant enzymolysis oligopeptide of North Pacific squid spawn tangled gland, it is characterised in that：By weight containing such as Anti-oxidant enzymolysis oligopeptide 0.01-0.5 parts of North Pacific's squid spawn tangled gland, 1-3 parts of barrenwort, Radix Astragali 1-5 described in claim 1 Part, 1-10 parts of Ramulus Taxilli, 10-20 parts of the red sage root, 1-10 parts of radish seed, 2-10 parts of Oldenlandia diffusa Roxb, 3-20 parts of the root of kudzu vine.
CN201710160381.3A 2017-03-17 2017-03-17 Antioxidative enzymolysis oligopeptide from peri-pacific squid oothecal gland Active CN107176973B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110759969A (en) * 2019-10-14 2020-02-07 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid

Citations (3)

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CN101983968A (en) * 2010-09-26 2011-03-09 浙江海洋学院 Preparation method of sepia oligopeptide by enzymatic hydrolysis and application thereof
CN103451257A (en) * 2013-06-17 2013-12-18 浙江海洋学院 Method for preparing polypeptide with high antioxidant activity through enzymolysis of internal organs of squids
CN106244657A (en) * 2016-08-29 2016-12-21 岭南师范学院 A kind of squid antioxidation polypeptide and its preparation method and application

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CN101983968A (en) * 2010-09-26 2011-03-09 浙江海洋学院 Preparation method of sepia oligopeptide by enzymatic hydrolysis and application thereof
CN103451257A (en) * 2013-06-17 2013-12-18 浙江海洋学院 Method for preparing polypeptide with high antioxidant activity through enzymolysis of internal organs of squids
CN106244657A (en) * 2016-08-29 2016-12-21 岭南师范学院 A kind of squid antioxidation polypeptide and its preparation method and application

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刘倩茹 等: "北太平洋鱿鱼缠卵腺抗氧化酶解寡肽的制备", 《湖北农业科学》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110759969A (en) * 2019-10-14 2020-02-07 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid
CN110759969B (en) * 2019-10-14 2021-10-22 浙江海洋大学 Preparation method of antioxidant enzymolysis oligopeptide from peripherical glands of northern pacific squid

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