CN201676560U - Novel chromatographic column - Google Patents
Novel chromatographic column Download PDFInfo
- Publication number
- CN201676560U CN201676560U CN2010201221831U CN201020122183U CN201676560U CN 201676560 U CN201676560 U CN 201676560U CN 2010201221831 U CN2010201221831 U CN 2010201221831U CN 201020122183 U CN201020122183 U CN 201020122183U CN 201676560 U CN201676560 U CN 201676560U
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- column
- filter
- column body
- fixed cap
- cylinder
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Abstract
The utility model discloses a novel chromatographic column which comprises a column body. An upper fixed cover is arranged outside the edge of an upper port of the column body; a lower fixed cover is arranged outside the edge of a lower port of the column body; a hollow adjusting column is arranged in the upper port of the column body and connected with the upper fixed cover through an upper end cover; a rubber plug is arranged at the lower end of the hollow adjusting column; a filter and a sealing gasket are arranged in the lower port of the column body; a lower end cover is arranged at the lower end of the column body and is in threaded connection with the filter through an adjusting tube; and a liquid outlet pipe is arranged at the lower part of the filter. The utility model is applicable to molecular sieves, is suitable for ion exchange, gel permeation and affinity chromatography of molecular sieves, has the advantages of advanced design, simple column assembly, small washing dead volume and good chemical resistance and is an optimal chromatographic tool piloted and massively produced in a biochemistry laboratory, a petroleum and chemical engineering laboratory, a chemical analysis laboratory, a disease diagnosis laboratory and other laboratories as well as in the chemical pharmaceutical process.
Description
Technical field
The utility model relates to the separator in fields such as biology, chemical industry, and particularly a kind of chromatographic column that is used for separation chemistry product and natural products experiment belongs to biochemical field.
Background technology
At present, the separation of chemical product or natural products mainly is to utilize normal pressure and decompression chromatographic column.The connector sealing property of normal pressure chromatographic column is relatively poor, is connected easy-to-leak liquid when using with the liquid feeding ball: with normal pressure chromatographic column wash-out the time, because its column body insufficient pressure, and can only use thick silica gel, and the wash-out consumed time is long and final effect is relatively poor.When using the decompression chromatographic column, need bleed with water pump, operate comparatively loaded down with trivial detailsly, and can produce unsettled suction when bleeding, cause the inhomogeneous wash-out of solution easily, and the solution proportion of separating out is lacked of proper care.The biochemical field development of China is swift and violent, directly driven the development of field of medicaments, constantly development, the medicine of producing and the technology of research and development have improved national medical condition, current diversified biology, medical purification production technique reach its maturity, and integral body has improved the application level of the molecular engineering of China at medicine, biology, chemical field.Natural protein replenishes and absorbs mainly from animal meat and cereal etc. as human body, for from refuse, extracting protein, become biochemical field and produced a big trend of protein, protein costs an arm and a leg also strict to the process of its production of purifying because of it, and output is extremely low, has not more and more satisfied the demand in market.Present compartment analysis protein, chromatography is important and common a kind of technology, the chromatography routine comprises 1, adsorpting column chromatography 2, ion-exchange chromatography, 3 gel chromatographies 4, affinity chromatography 5, chromatographic focusing.But because of the requirement of its technical conditions and instrument relatively stricter, so PR is lower, cost is than higher.
Summary of the invention
Deficiency at the prior art existence, technical problem to be solved in the utility model is, a kind of novel chromatographic column is provided, and the sealing of this novel chromatographic column is better, can leakage, operate comparatively easy, can control solution flow rate easily, internal pressure is stronger, and elution time is shorter, effect is better, and the solution proportion of separating out is comparatively even.
For solving the problems of the technologies described above, technical solution adopted in the utility model is: a kind of novel chromatographic column comprises cylinder, fixed cap on the described cylinder upper port edge arranged outside, fixed cap under the cylinder lower port edge arranged outside; Be provided with the hollow adjustable column in the described cylinder upper port, the hollow adjustable column is connected with last fixed cap by upper end cover; Described hollow adjustable column lower end is provided with rubber stopper; Be provided with filter and sealing gasket in the described cylinder lower port; Described cylinder lower end is provided with bottom end cover, and bottom end cover is threaded with filter by adjustable pipe with filter; Described filter bottom is provided with drain pipe.
Optimize, above-mentioned a kind of novel chromatographic column, described going up between fixed cap and the cylinder is provided with sealing ring, is provided with sealing ring between following fixed cap and the cylinder.
Optimize, be connected by screw bolt and nut between the above-mentioned a kind of novel chromatographic column, described upper end cover and last fixed cap.
The utility model is applicable to molecular sieve, ion-exchange, gel infiltration and affinity chromatography, advanced design, the dress post is easy, wash-out " dead volume " is little, has good chemical resistance, is laboratory and the pilot scale of chemical pharmacy and the best chromatography instruments of large-scale production such as biochemistry, petrochemical industry, chemical analysis, medical diagnosis on disease.The utility model is rational in infrastructure, and is easy to use, can protect the gel face not to be subjected to the destruction of liquid stream, and chromatographic flow is evenly distributed, thereby can improve resolution ratio.Compared with prior art, the utility model is made into standard frosted mouth with the connector of cylinder upper end, makes that like this sealing was fine when it was connected use with liquid feeding ball or pressue device, can not produce the phenomenon of leakage.Because solution is in the uniform pressure effect of column body current downflow, make that the solution proportion of separating out is comparatively even.
Description of drawings
Fig. 1 is a structural representation of the present utility model.
The specific embodiment
Below in conjunction with accompanying drawing and embodiment the utility model is further described.
As shown in Figure 1, a kind of novel chromatographic column comprises cylinder 1, fixed cap 2 on the described cylinder 1 upper port edge arranged outside, fixed cap 3 under the cylinder 1 lower port edge arranged outside; Be provided with hollow adjustable column 4 in described cylinder 1 upper port, hollow adjustable column 4 is connected with last fixed cap 2 by upper end cover 5; Described hollow adjustable column 4 lower ends are provided with rubber stopper 6; Be provided with filter 7 and sealing gasket 8 in described cylinder 1 lower port; Described cylinder 1 lower end is provided with bottom end cover 9, and bottom end cover 9 is threaded with filter 7 by adjustable pipe 8 with filter 7; Described filter 7 bottoms are provided with drain pipe 10.Described going up between fixed cap 2 and the cylinder 1 is provided with sealing ring 11, is provided with sealing ring 11 between following fixed cap 3 and the cylinder 1.Be connected with nut 13 by bolt 12 between described upper end cover 5 and the last fixed cap 2.During use, can select no glue can carry out the chromatography of each material as required, after the preparation separation was finished, the screw bolt and nut of outwarding winding was a various piece capable of washing, in order to using next time again.
Certainly, above-mentioned explanation is not to be to restriction of the present utility model, and the utility model also is not limited to above-mentioned giving an example; those skilled in the art; in essential scope of the present utility model, the variation of making, remodeling, interpolation or replacement all should belong to protection domain of the present utility model.
Claims (3)
1. a novel chromatographic column comprises cylinder, it is characterized in that: fixed cap on the described cylinder upper port edge arranged outside, fixed cap under the cylinder lower port edge arranged outside; Be provided with the hollow adjustable column in the described cylinder upper port, the hollow adjustable column is connected with last fixed cap by upper end cover; Described hollow adjustable column lower end is provided with rubber stopper; Be provided with filter and sealing gasket in the described cylinder lower port; Described cylinder lower end is provided with bottom end cover, and bottom end cover is threaded with filter by adjustable pipe with filter; Described filter bottom is provided with drain pipe.
2. a kind of novel chromatographic column according to claim 1 is characterized in that: described going up between fixed cap and the cylinder is provided with sealing ring, is provided with sealing ring between following fixed cap and the cylinder.
3. a kind of novel chromatographic column according to claim 1 is characterized in that: be connected by screw bolt and nut between described upper end cover and the last fixed cap.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201221831U CN201676560U (en) | 2010-03-03 | 2010-03-03 | Novel chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201221831U CN201676560U (en) | 2010-03-03 | 2010-03-03 | Novel chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201676560U true CN201676560U (en) | 2010-12-22 |
Family
ID=43341624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201221831U Expired - Fee Related CN201676560U (en) | 2010-03-03 | 2010-03-03 | Novel chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201676560U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225248A (en) * | 2011-03-29 | 2011-10-26 | 中国科学院过程工程研究所 | Sealing assembly of chromatography column |
| CN105556302A (en) * | 2013-09-27 | 2016-05-04 | 通用电气健康护理生物科学股份公司 | Chromatography column assembly |
| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
-
2010
- 2010-03-03 CN CN2010201221831U patent/CN201676560U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102225248A (en) * | 2011-03-29 | 2011-10-26 | 中国科学院过程工程研究所 | Sealing assembly of chromatography column |
| CN102225248B (en) * | 2011-03-29 | 2013-10-16 | 中国科学院过程工程研究所 | Sealing assembly of chromatography column |
| CN105556302A (en) * | 2013-09-27 | 2016-05-04 | 通用电气健康护理生物科学股份公司 | Chromatography column assembly |
| US10315134B2 (en) | 2013-09-27 | 2019-06-11 | Ge Healthcare Bio-Science Ab | Chromatography column assembly with snap fit end cap and adaptor shaft |
| CN110327661A (en) * | 2013-09-27 | 2019-10-15 | 通用电气健康护理生物科学股份公司 | Chromatographic column component |
| CN110960886A (en) * | 2019-11-11 | 2020-04-07 | 四川大学 | High-speed counter-current chromatographic separation column |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101222 Termination date: 20130303 |