CN103694312A - Protein purification chromatographic system - Google Patents
Protein purification chromatographic system Download PDFInfo
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- CN103694312A CN103694312A CN201310644926.XA CN201310644926A CN103694312A CN 103694312 A CN103694312 A CN 103694312A CN 201310644926 A CN201310644926 A CN 201310644926A CN 103694312 A CN103694312 A CN 103694312A
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Abstract
The invention provides a protein purification chromatographic system, belonging to bio-purification devices. The chromatographic system provided by the invention comprises a sample injection liquid storage system and a sample discharge liquid storage system which both comprise multiple channels and are connected with subordinate or superior equipment through electromagnetic valves. The electromagnetic valves used are connected with a computer system in high degree of automation. The electromagnetic valves can be automatically opened or closed so as to automatically and continuously realize four steps: sampling, washing, desorption and column regeneration.
Description
Technical field
The invention belongs to biologically pure device, be specifically related to a kind of chromatographic system of protein purification.
Background technology
At present, the biochemical field development of China is swift and violent, directly driven the development of field of medicaments, constantly development, the medicine of producing and the technology of research and development have improved national medical condition, current diversified biology, medical purification production technique reach its maturity, and integral body has improved the molecular engineering of China in the application level of medicine, biology, chemical field.
Protein is as forming the requisite component of human body, be described as the living matter of human body, natural protein supplementing and absorbing mainly from animal meat and cereal etc. as human body, for extract protein from refuse, become biochemical field and produced a large trend of protein, protein is expensive also strict to the mistake range request of its production of purifying because of it, and output is extremely low, more and more can not meet the demand in market.
Current protein purification system is generally only applicable to the technical study operation in laboratory; Level of automation is low, can not carry out continuously the operation of loading, washing, desorb and column regeneration; In purge process, human factor affects greatly, poor repeatability; Can not meet those technical maturities, can be used for the chromatographic system that protein purification is produced.
Summary of the invention
For addressing the above problem, the invention provides a kind of chromatographic system of protein purification, adopt following technical scheme:
A kind of chromatographic system of protein purification, comprise sample introduction liquid storage system, chromatography column and go out sample liquid storage system, it is characterized in that, described sample introduction liquid storage system comprises sample liquid hold-up vessel, washings hold-up vessel, stripping liquid hold-up vessel and regenerated liquid hold-up vessel; Described sample liquid hold-up vessel, washings hold-up vessel and stripping liquid hold-up vessel are connected with constant flow pump by magnetic valve respectively, and described regenerated liquid hold-up vessel is connected with acid and alkali-proof pump by magnetic valve; The other end of described constant flow pump and acid and alkali-proof pump is connected with the ingress of chromatography column by Y-tube; The described sample liquid storage system that goes out comprises waste liquid tank, intersection sample tank and qualified sample tank, and described waste liquid tank, the sample tank that intersects are connected with the outlet of chromatography column by magnetic valve respectively with qualified sample tank.
Preferably, the inside of described sample liquid hold-up vessel arranges elution device.
Beneficial effect of the present invention is as follows:
1. chromatographic system provided by the invention, sample introduction liquid storage system is all multichannel with going out sample liquid storage system, and magnetic valve is all set to be connected with subordinate or higher level equipment, magnetic valve used is all connected with computer system, level of automation is high, can automatically open or shut electromagnetic valve, with automatization ground, complete loading, washing, desorb and column regeneration four procedures continuously.
2. chromatographic system provided by the invention, be provided with intersection sample tank, for the liquid of collecting washing process, containing a small amount of sample, then the liquid of collecting in intersection sample tank is carried out to the adjusting of pH value, ionic concn etc., then just can carry out again the step of loading and chromatography purification, avoid the waste of target protein.
3. chromatographic system provided by the invention, manual operations less, reduce labor force; Reproducible, the albumen that is especially applicable to those purifying process maturations carries out mass purification production.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents sample liquid hold-up vessel; 2 represent washings hold-up vessel; 3 represent stripping liquid hold-up vessel; 4 represent regenerated liquid hold-up vessel; 5 represent constant flow pump; 6 represent acid and alkali-proof pump; 7 represent chromatography column; 8 represent waste liquid tank; 9 represent intersection sample tank; 10 represent qualified sample tank.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1, a kind of chromatographic system of protein purification, comprise sample introduction liquid storage system, chromatography column 7 and go out sample liquid storage system, it is characterized in that, described sample introduction liquid storage system comprises sample liquid hold-up vessel 1, washings hold-up vessel 2, stripping liquid hold-up vessel 3 and regenerated liquid hold-up vessel 4; Described sample liquid hold-up vessel 1, washings hold-up vessel 2 and stripping liquid hold-up vessel 3 are connected with constant flow pump 5 by magnetic valve respectively, and described regenerated liquid hold-up vessel 4 is connected with acid and alkali-proof pump 6 by magnetic valve; The other end of described constant flow pump 5 and acid and alkali-proof pump 6 is connected with the ingress of chromatography column 7 by Y-tube; The described sample liquid storage system that goes out comprises waste liquid tank 8, intersection sample tank 9 and qualified sample tank 10, and described waste liquid tank 8, the sample tank 9 that intersects are connected with the outlet of chromatography column 7 by magnetic valve respectively with qualified sample tank 10.
As preferably, the inside of sample liquid hold-up vessel 1 arranges elution device.
Chromatographic system provided by the invention, its principle of work is as follows:
Loading: the magnetic valve of sample liquid hold-up vessel and waste liquid tank is opened, started constant flow pump, complete loading operation;
Washing: the magnetic valve of washings hold-up vessel and intersection sample tank is opened, started constant flow pump, complete washing operation;
Desorb: the magnetic valve of stripping liquid hold-up vessel and qualified sample tank is opened, started constant flow pump, complete desorb operation;
Column regeneration: the magnetic valve of regenerated liquid hold-up vessel and waste liquid tank is opened, started acid and alkali-proof pump, complete the operation of chromatography column regeneration.
Claims (2)
1. the chromatographic system of a protein purification, comprise sample introduction liquid storage system, chromatography column (7) and go out sample liquid storage system, it is characterized in that, described sample introduction liquid storage system comprises sample liquid hold-up vessel (1), washings hold-up vessel (2), stripping liquid hold-up vessel (3) and regenerated liquid hold-up vessel (4); Described sample liquid hold-up vessel (1), washings hold-up vessel (2) and stripping liquid hold-up vessel (3) are connected with constant flow pump (5) by magnetic valve respectively, and described regenerated liquid hold-up vessel (4) is connected with acid and alkali-proof pump (6) by magnetic valve; The other end of described constant flow pump (5) and acid and alkali-proof pump (6) is connected with the ingress of chromatography column (7) by Y-tube; The described sample liquid storage system that goes out comprises waste liquid tank (8), intersection sample tank (9) and qualified sample tank (10), and described waste liquid tank (8), the sample tank (9) that intersects are connected with the outlet of chromatography column (7) by magnetic valve respectively with qualified sample tank (10).
2. the chromatographic system of protein purification according to claim 1, is characterized in that, the inside of described sample liquid hold-up vessel (1) arranges elution device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644926.XA CN103694312A (en) | 2013-12-05 | 2013-12-05 | Protein purification chromatographic system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644926.XA CN103694312A (en) | 2013-12-05 | 2013-12-05 | Protein purification chromatographic system |
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| Publication Number | Publication Date |
|---|---|
| CN103694312A true CN103694312A (en) | 2014-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310644926.XA Pending CN103694312A (en) | 2013-12-05 | 2013-12-05 | Protein purification chromatographic system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987122A (en) * | 2017-12-27 | 2018-05-04 | 湖北普罗金科技有限公司 | Separation of Proteins extraction element |
| CN108164582A (en) * | 2017-12-27 | 2018-06-15 | 湖北普罗金科技有限公司 | A kind of protein purification device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202126430U (en) * | 2011-05-18 | 2012-01-25 | 江苏奥迪康医学科技有限公司 | Glycated hemoglobin four gradient elute analyzer |
| CN103159824A (en) * | 2013-02-06 | 2013-06-19 | 中国科学院生物物理研究所 | Full sealed pipelined protein purification system and application on preparation of sterile apyrogeneity protein pharmaceutical medicine |
-
2013
- 2013-12-05 CN CN201310644926.XA patent/CN103694312A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202126430U (en) * | 2011-05-18 | 2012-01-25 | 江苏奥迪康医学科技有限公司 | Glycated hemoglobin four gradient elute analyzer |
| CN103159824A (en) * | 2013-02-06 | 2013-06-19 | 中国科学院生物物理研究所 | Full sealed pipelined protein purification system and application on preparation of sterile apyrogeneity protein pharmaceutical medicine |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107987122A (en) * | 2017-12-27 | 2018-05-04 | 湖北普罗金科技有限公司 | Separation of Proteins extraction element |
| CN108164582A (en) * | 2017-12-27 | 2018-06-15 | 湖北普罗金科技有限公司 | A kind of protein purification device |
| CN107987122B (en) * | 2017-12-27 | 2023-10-13 | 湖北普罗金科技有限公司 | Protein separation and extraction device |
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Application publication date: 20140402 |