CN202126430U - Glycated hemoglobin four gradient elute analyzer - Google Patents
Glycated hemoglobin four gradient elute analyzer Download PDFInfo
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- CN202126430U CN202126430U CN2011201588347U CN201120158834U CN202126430U CN 202126430 U CN202126430 U CN 202126430U CN 2011201588347 U CN2011201588347 U CN 2011201588347U CN 201120158834 U CN201120158834 U CN 201120158834U CN 202126430 U CN202126430 U CN 202126430U
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- peristaltic pump
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- glycosylated hemoglobin
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Abstract
The utility model belongs to medical equipment and relates to a glycated hemoglobin four gradient elute analyzer for detecting concentration of glycated hemoglobin through ion-exchange column chromatography. The glycated hemoglobin four gradient elute analyzer comprises a peristaltic pump installed on a liquid supplying pipeline, a liquid outlet of the peristaltic pump sequentially passes through a cation exchange resin chromatographic column and a colorimetric cell and then enters a waste liquid bottle after colorimetric analyzing. A liquid inlet of the peristaltic pump is connected with four eluent containers which are independently and respectively connected with the liquid inlet of the peristaltic pump through valves. The glycated hemoglobin four gradient elute analyzer is simple in structure, reliable in equipment operation, accurate in detecting, low in manufacturing cost and free of cross contamination of the eluent.
Description
Technical field
The utility model belongs to Medical Devices, relates to the glycosylated hemoglobin four gradient elution analysis appearance that a kind of ion exchange chromatography detects glycosylated hemoglobin concentration.
Background technology
The concentration of glycosylated hemoglobin is the important clinical index of diabetes diagnosis in the blood of human body.Hb A hemoglobin adult (Hb) can be formed multiple subfraction after the saccharification, but topmost subfraction is HbA
1c, it forms the back Stability Analysis of Structures, and 90～120 days life cycle is arranged.Through detecting this index, can reflect the trimestral average blood sugar level of diabetes patient.Therefore, clinically generally with the important evidence of this index as diabetes patient's examination, diagnosis, treatment.
The assay method of existing glycosylated hemoglobin has ion exchange chromatography, electrophoresis, immunoturbidimetry and affinity chromatography.Topmost method is an ion exchange chromatography.
Can cause haemoglobin molecule surface kation to be lost after adopting ion exchange chromatography to be based on the haemoglobin saccharification.When containing the sample process cation-exchange chromatography post of glycosylated hemoglobin, glycosylated hemoglobin and non-glycosylated hemoglobin are all adsorbed by Zeo-karb.Because of positively charged the having nothing in common with each other of the glycosylated hemoglobin of different subfractions and non-glycated hemoglobin molecules surface, so also have nothing in common with each other with the absorption affinity of Zeo-karb.With the eluent of different ions concentration, take gradient elution method from low to high, wash-out goes out the glycosylated hemoglobin and the non-glycosylated hemoglobin of different subfractions one by one.
The method that at present adopts ion-exchange liquid phase chromatography method to separate glycosylated hemoglobin clinically wherein is divided into ion-exchange high efficiency liquid phase chromatographic analysis method (HPLC) and ion-exchange low pressure liquid phase chromatographic analysis method (LPLC) again.Wherein, ion-exchange high efficiency liquid phase chromatographic analysis method (HPLC) is the internationally recognized full standard method of analysis of glycosylated hemoglobin HbA1c.Above-mentioned two kinds of methods all only adopt the eluent of two kinds of ion concentrations for can isolate multiple glycosylated hemoglobin subfraction, promptly a kind of high ion concentration eluent and a kind of low ion concns eluent.In use, be through the eluent that one mechanical six logical rotary distributor comes proportioning generation different ions concentration is set in the machine, so that sample is carried out wash-out.Theoretically, the ion concentration kind that proportioning goes out is many more, and the subfraction liquid of ability wash-out is many more.But in the clinical practice of most of occasions, the index that really has directive significance has only HbA
1c, other subfractions do not have too big practical significance to the examination of diabetes clinically, the inspection of result of treatment, generally can only be used for research or special case, thereby the isolated most of results of existing equipment are to clinical directive significance and little.But because existing equipment adopts proportioning variable concentrations eluent in the machine, make that the complexity of instrument increases, failure rate increases, the cross pollution of eluent is serious, and the cost of the manufacturing of instrument, maintenance, use is also higher.
Summary of the invention
The utility model technical matters to be solved is, a kind of simple in structure, equipment operation reliable, detect accurately, low cost of manufacture, eluent do not have cross pollution glycosylated hemoglobin four gradient elution analysis appearance are provided.
The glycosylated hemoglobin four gradient elution analysis appearance of the utility model include the peristaltic pump that is installed on the feed flow pipeline, and the liquid outlet of said peristaltic pump is analysed post and colorimetric pool through cation exchange resin layer successively, after colorimetric analysis, gets into waste liquid bottle; The inlet of said peristaltic pump is connected with four eluent containers, and described four eluent containers are respectively
A eluent container holds the A eluent that is used for Zeo-karb cleaning, balance;
B eluent container holds and is used for other subfractions of wash-out glycosylated hemoglobin HbA
1a, HbA
1bThe B eluent;
C eluent container holds and is used for wash-out glycosylated hemoglobin HbA
1cThe C eluent of subfraction;
D eluent container holds the D eluent that is used for non-glycosylated hemoglobin of wash-out and cation regenerant exchange resin;
Said A eluent container, B eluent container, C eluent container and D eluent container are connected with the peristaltic pump inlet through valve respectively independently of one another.
Valve V1-2, valve V2-2 and valve V3-2 that said A eluent container is connected with being provided with of peristaltic pump inlet; Said B eluent container and peristaltic pump inlet valve V3-1 is set; Said C eluent container and peristaltic pump inlet valve V2-1 is set; Said D eluent container and peristaltic pump inlet valve V1-1 is set; Said valve V1-1 and valve V1-2, valve V2-1 and valve V2-2, valve V3-1 and valve V3-2 are three pairs of interstage valves, and the open and close opposite states of two valves in the every pair of interstage valve.
The utility model is owing to adopt simple flow path system just can realize the glycosylated hemoglobin HbA that has only high performance liquid chromatography (HPLC) under condition of high voltage, could realize under the condition of normal pressure
1cSeparation accuracy; Baroque six logical rotary distributors have also been avoided for the necessary employing of proportioning different kinds of ions concentration eluent; Eliminated the cross pollution influence between the variable concentrations eluent, can make the ion concentration of eluent more accurately reliable, glycosylated hemoglobin HbA
1cThe separation accuracy of subfraction is higher.
Because the concentration of the glycosylated hemoglobin in the blood of human body is the important clinical index of diagnosing diabetes.Hb A hemoglobin adult in the blood of human body (Hb) is made up of three kinds of haemoglobin subfractions: hemoglobin A (HbA), Hemoglobin F (HbF), HbA2 (HbA2).Mainly contain hemoglobin A (HbA) among the HbA, account for 95%～97% of whole haemoglobin.And hemoglobin A (HbA) is made up of two kinds of subfractions: glycosylated hemoglobin (HbA1) (5%～7%) and non-glycosylated hemoglobin (HbA0) (90%).Glycosylated hemoglobin (HbA1) be haemoglobin (Hb) with carbohydrate (like glucose, 6-glucose 1-phosphate1-or 1.6-diphosphofructose) through non-enzymatic be combined into.In glycosylated hemoglobin (HbA1), it is external to remove a small amount of other Different Variation of existence, and three kinds of subfractions are mainly also arranged, and is respectively: HbA1a, HbA1b and HbA1c.Wherein, HbA1c accounts for 75%～80% of HbA1, and Stability Analysis of Structures.Therefore, through the utility model each subfraction of glycosylated hemoglobin (HbA1) and non-glycosylated hemoglobin (HbA0) being carried out that accurate wash-out separates just can be through the concentration of theoretical derivation acquisition glycosylated hemoglobin.
Through the test contrast, the testing result of the utility model can satisfy the needs of clinical diagnosis fully.
Description of drawings
Fig. 1 is the flow path system schematic diagram of the utility model.
Embodiment
As shown in the figure, glycosylated hemoglobin four gradient elution analysis appearance include the peristaltic pump 1 that is installed on the feed flow pipeline, and the liquid outlet of said peristaltic pump is analysed post 3 and colorimetric pool 4 through cation exchange resin layer successively, after colorimetric analysis, get into waste liquid bottle 5; The inlet of said peristaltic pump 1 is connected with four eluent containers, and described four eluent containers are respectively
A eluent container 6 holds the A eluent that is used for Zeo-karb cleaning, balance;
B eluent container 7 holds and is used for other subfractions of wash-out glycosylated hemoglobin HbA
1a, HbA
1bThe B eluent;
C eluent container 8 holds and is used for wash-out glycosylated hemoglobin HbA
1cThe C eluent of subfraction;
D eluent container 9 holds the D eluent that is used for non-glycosylated hemoglobin of wash-out and cation regenerant exchange resin;
Said A eluent container 6, B eluent container 7, C eluent container 8 are connected with the peristaltic pump inlet through valve respectively with D eluent container 9 independently of one another.
Valve V1-2, valve V2-2 and valve V3-2 that said A eluent container is connected with being provided with of peristaltic pump inlet; Said B eluent container and peristaltic pump inlet valve V3-1 is set; Said C eluent container and peristaltic pump inlet valve V2-1 is set; Said D eluent container and peristaltic pump inlet valve V1-1 is set; Said valve V1-1 and valve V1-2, valve V2-1 and valve V2-2, valve V3-1 and valve V3-2 are three pairs of interstage valves, and the open and close opposite states of two valves in the every pair of interstage valve.That is, when having only B eluent container, C eluent container and D eluent container all to be closed, just the A eluent feeds peristaltic pump.
In its course of work, different eluents is analysed post 3 through the cation exchange resin layer that has adsorbed sample to be tested respectively under the effect of peristaltic pump 1, gets into colorimetric pool 4 behind the wash-out and carries out stratographic analysis.
Claims (2)
1. glycosylated hemoglobin four gradient elution analysis appearance; Include the peristaltic pump (1) that is installed on the feed flow pipeline; The liquid outlet of said peristaltic pump analyses post (3) through cation exchange resin layer successively and colorimetric pool (4) gets into waste liquid bottle (5); It is characterized in that: the inlet of said peristaltic pump (1) is connected with four eluent containers, and described four eluent containers are respectively
A eluent container (6) holds the A eluent that is used for Zeo-karb cleaning, balance;
B eluent container (7) holds and is used for other subfractions of wash-out glycosylated hemoglobin HbA
1a, HbA
1bThe B eluent;
C eluent container (8) holds and is used for wash-out glycosylated hemoglobin HbA
1cThe C eluent of subfraction;
D eluent container (9) holds the D eluent that is used for non-glycosylated hemoglobin of wash-out and cation regenerant exchange resin;
Said A eluent container (6), B eluent container (7), C eluent container (8) and D eluent container (9) are connected with the peristaltic pump inlet through valve respectively independently of one another.
2. glycosylated hemoglobin four gradient elution analysis appearance according to claim 1 is characterized in that: valve V1-2, valve V2-2 and valve V3-2 that said A eluent container is connected with being provided with of peristaltic pump inlet; Said B eluent container and peristaltic pump inlet valve V3-1 is set; Said C eluent container and peristaltic pump inlet valve V2-1 is set; Said D eluent container and peristaltic pump inlet valve V1-1 is set; Said valve V1-1 and valve V1-2, valve V2-1 and valve V2-2, valve V3-1 and valve V3-2 are three pairs of interstage valves, and the open and close opposite states of two valves in the every pair of interstage valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201588347U CN202126430U (en) | 2011-05-18 | 2011-05-18 | Glycated hemoglobin four gradient elute analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011201588347U CN202126430U (en) | 2011-05-18 | 2011-05-18 | Glycated hemoglobin four gradient elute analyzer |
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| Publication Number | Publication Date |
|---|---|
| CN202126430U true CN202126430U (en) | 2012-01-25 |
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| CN2011201588347U Expired - Fee Related CN202126430U (en) | 2011-05-18 | 2011-05-18 | Glycated hemoglobin four gradient elute analyzer |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102861460A (en) * | 2012-09-26 | 2013-01-09 | 杜康 | High performance column chromatography separator |
| CN103694312A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Protein purification chromatographic system |
| CN103694311A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatography device for purifying protein |
| CN103884798A (en) * | 2014-04-14 | 2014-06-25 | 江苏奥迪康医学科技有限公司 | Glycosylated hemoglobin analyzer reagent matching device as well as operating method thereof |
| CN103969375A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | An elution method for liquid chromatography and inspection equipment thereof |
| CN103969349A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | A washing method of inspection equipment and the inspection equipment thereof |
| CN105974141A (en) * | 2016-05-09 | 2016-09-28 | 温冬梅 | Novel multi-mode automatic analysis system of glycated hemoglobin HbA1c |
| CN109060992A (en) * | 2018-09-03 | 2018-12-21 | 大连技嘉科技有限公司 | The glycolated hemoglobin analysis of detection accuracy can be improved |
-
2011
- 2011-05-18 CN CN2011201588347U patent/CN202126430U/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102861460A (en) * | 2012-09-26 | 2013-01-09 | 杜康 | High performance column chromatography separator |
| CN103969375A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | An elution method for liquid chromatography and inspection equipment thereof |
| CN103969349A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | A washing method of inspection equipment and the inspection equipment thereof |
| CN103694312A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Protein purification chromatographic system |
| CN103694311A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatography device for purifying protein |
| CN103884798A (en) * | 2014-04-14 | 2014-06-25 | 江苏奥迪康医学科技有限公司 | Glycosylated hemoglobin analyzer reagent matching device as well as operating method thereof |
| CN103884798B (en) * | 2014-04-14 | 2015-09-09 | 江苏奥迪康医学科技有限公司 | Glycolated hemoglobin analysis reagent coalignment and method of work thereof |
| CN105974141A (en) * | 2016-05-09 | 2016-09-28 | 温冬梅 | Novel multi-mode automatic analysis system of glycated hemoglobin HbA1c |
| CN109060992A (en) * | 2018-09-03 | 2018-12-21 | 大连技嘉科技有限公司 | The glycolated hemoglobin analysis of detection accuracy can be improved |
| CN109060992B (en) * | 2018-09-03 | 2024-02-27 | 苏州博睿嘉晟医疗科技有限公司 | Glycosylated hemoglobin analyzer capable of improving detection accuracy |
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Legal Events
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|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee after: JIANGSU AUDICOM MEDICAL TECHNOLOGY CO., LTD. Address before: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee before: Jiangsu Audicom Medical Technology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120125 Termination date: 20200518 |
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| CF01 | Termination of patent right due to non-payment of annual fee |