CN102226788B - Four-gradient elution analysis method of glycated hemoglobin analyzer - Google Patents
Four-gradient elution analysis method of glycated hemoglobin analyzer Download PDFInfo
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Abstract
The invention relates to a four-gradient elution analysis method of a glycated hemoglobin analyzer, which is used for detecting concentration of glycated hemoglobin via ionic exchange chromatography. The method comprises the following steps of: setting four kinds of eluent with fixed concentration; respectively using the four kinds of eluent to elute cation exchange resin having an absorbed sample to be detected; and performing chromatographic analysis on the eluted liquid to determine the content of the eluted ingredients, thereby determining a blood glucose concentration, wherein the four kinds of eluent are respectively as follows: an eluent A for washing and balancing the cation exchange resin, an eluent B for eluting other subfractions including HbA1a and HbA1b of the hemoglobin, an eluent C for eluting HbA1c subfraction of the glycated hemoglobin, and an eluent D for eluting an non-glycated hemoglobin and regenerated cation exchange resin. The method provided by the invention can accurately detect the concentration of the glycated hemoglobin, simplify structures of detection devices and detection programs, improve operational reliability of devices, decrease cost on devices and reduce cross contamination of the eluent.
Description
Technical field
The present invention relates to the glycolated hemoglobin analysis four gradient elution analysis methods that a kind of ion exchange chromatography detects glycosylated hemoglobin concentration.
Background technology
The concentration of glycosylated hemoglobin is the important clinical index of diabetes diagnosis in the blood of human body.Hb A hemoglobin adult (Hb) can be formed multiple subfraction after the saccharification, but topmost subgroup is divided into HbA
1c, Stability Analysis of Structures after it forms has 90～120 days life cycle.By detecting this index, can reflect the trimestral average blood sugar level of diabetes patient.Therefore, clinically generally with the important evidence of this index as diabetes patient's examination, diagnosis, treatment.
The assay method of glycosylated hemoglobin has ion exchange chromatography, electrophoresis, immunoturbidimetry and affinity chromatography now.Topmost method is ion exchange chromatography.
Can cause haemoglobin molecule surface kation to be lost after adopting ion exchange chromatography to be based on the haemoglobin saccharification.When containing the sample process cation-exchange chromatography post of glycosylated hemoglobin, glycosylated hemoglobin and non-glycosylated hemoglobin are all adsorbed by Zeo-karb.Because of the glycosylated hemoglobin of different subfractions and non-glycated hemoglobin molecules surface institute positively charged different, so also different with the absorption affinity of Zeo-karb.With the eluent of different ions concentration, take gradient elution method from low to high, can elute one by one glycosylated hemoglobin and the non-glycosylated hemoglobin of different subfractions.
The method that at present adopts clinically ion-exchange liquid phase chromatography to separate glycosylated hemoglobin wherein is divided into again Ion-exchange high-performance liquid chromatography analytic approach (HPLC) and ion-exchange low pressure liquid phase chromatography analytic approach (LPLC).Wherein, Ion-exchange high-performance liquid chromatography analytic approach (HPLC) is the internationally recognized goldstandard analytical approach of Glycohemoglobin HbA1c.Above-mentioned two kinds of methods all only adopt the eluent of two kinds of ion concentrations for can isolate multiple glycosylated hemoglobin subfraction, i.e. a kind of high ion concentration eluent and a kind of low ion concns eluent.In use, be the eluent that comes proportioning generation different ions concentration by one mechanical six logical rotary distributor is set in the machine, so that sample is carried out wash-out.Theoretically, the ion concentration kind that proportioning goes out is more, and the subfraction of energy wash-out is more.Therefore the high pressure liquid chromatography (HPLC) method, generally can go out the nearly subfraction of tens kinds of glycosylated hemoglobins by chromatography because employing can tolerate nearly 180 atmospheric ion exchange columns.And the instrument of low pressure liquid chromatography method though pressure is normal pressure, also can be isolated nearly 2-3 kind glycosylated hemoglobin subfraction.And in the clinical practice, the index that really has directive significance only has HbA
1c, other subfractions do not have too large practical significance to the examination of diabetes clinically, the inspection of result for the treatment of, generally can only be used for research or special case.Thereby isolated most of result is to clinical directive significance and little.But because proportioning variable concentrations eluent in the employing machine, must bring the complexity of instrument to increase, failure rate increases, the cross pollution of eluent is serious, the cost of the manufacturing of instrument, maintenance, use is also high.
Summary of the invention
Technical matters to be solved by this invention is, provide a kind of and accurately detecting glycosylated hemoglobin concentration simultaneously, can simplify assay device structures and trace routine, raising equipment operational reliability, reduce equipment cost, can reduce the glycolated hemoglobin analysis four gradient elution analysis methods of eluent cross pollution simultaneously.
Method of the present invention is: the eluent that four kinds of fixed concentrations are set, make it have the Zeo-karb of sample to be tested to carry out wash-out to absorption respectively, and the liquid behind the wash-out carried out stratographic analysis, determine the content of wash-out composition, and then definite glycosylated hemoglobin concentration;
Described four kinds of eluents are respectively:
The A eluent is used for Zeo-karb cleaning, balance; Its composition and percentage by weight are: NaH
2PO
40.146～0.166%, Na
2HPO
40.035～0.055%, NaCl 0.022～0.024%, KCl 0.032～0.034%, NaN
30.019～0.021%, deionized water surplus;
The B eluent is used for other subfractions of wash-out glycosylated hemoglobin HbA
1a, HbA
1bIts composition and weight ratio number percent are: NaH
2PO
40.124～0.144%, Na
2HPO
40.043～0.063%, NaCl 0.341～0.361%, KCl 0.034～0.036%, NaN
30.019～0.021%, deionized water surplus;
The C eluent is used for wash-out glycosylated hemoglobin HbA
1cSubfraction; Its composition and weight ratio number percent are: NaH
2PO
40.118～0.138%, Na
2HPO
40.039～0.041%, NaCl0.688～0.708%, KCl 0.072～0.074%, NaN
30.019～0.021%, deionized water surplus;
D eluent: be used for the non-glycosylated hemoglobin of wash-out and cation regenerant exchange resin; Its composition and weight ratio number percent are: NaH
2PO
40.1～0.12%, Na
2HPO
40.064～0.074%, NaCl 1.605～1.805%, NaN
30.019～0.021%, deionized water surplus;
Adopt the instrument of the inventive method also only to need to adopt common solenoid valve and simple flow path system just can realize the glycosylated hemoglobin HbA that only has high performance liquid chromatography (HPLC) under condition of high voltage, could realize under the condition of normal pressure
1cSeparation accuracy, baroque six logical rotary distributors for the necessary employing of proportioning different kinds of ions concentration eluent have also been avoided, eliminated the cross pollution impact between the variable concentrations eluent, can make the ion concentration of eluent more accurate reliable, glycosylated hemoglobin HbA
1cThe separation accuracy of subfraction is higher.
Because the concentration of the glycosylated hemoglobin in the blood of human body is the important clinical index of diagnosing diabetes.Hb A hemoglobin adult in the blood of human body (Hb) is comprised of three kinds of haemoglobin subfractions: hemoglobin A (HbA), Hemoglobin F (HbF), HbA2 (HbA2).Mainly contain hemoglobin A (HbA) among the HbA, account for 95%～97% of whole haemoglobin.And hemoglobin A (HbA) is comprised of two kinds of subfractions: glycosylated hemoglobin (HbA1) (5%～7%) and non-glycosylated hemoglobin (HbA0) (90%).Glycosylated hemoglobin (HbA1) is that haemoglobin (Hb) is combined into through non-enzymatic with carbohydrate (such as glucose, G6P or 1.6-diphosphofructose).In glycosylated hemoglobin (HbA1), a small amount of other Different Variation are external except existing, and three kinds of subfractions are mainly also arranged, and are respectively: HbA1a, HbA1b and HbA1c.Wherein, HbA1c accounts for 75%～80% of HbA1, and Stability Analysis of Structures.Therefore, by method of the present invention each subfraction of glycosylated hemoglobin (HbA1) and non-glycosylated hemoglobin (HbA0) being carried out that accurate wash-out separates just can be by the concentration of the theoretical acquisition glycosylated hemoglobin of deriving.
Through Experimental Comparison, the testing result of the inventive method can satisfy the needs of clinical diagnosis fully.
Description of drawings
Fig. 1 is the checkout equipment schematic diagram that the inventive method embodiment adopts.
Embodiment
In checkout equipment shown in Figure 1, four kinds of independently eluents are set, that is: A eluent 1, B eluent 2, C eluent 3, D eluent 4, the export pipeline of four kinds of eluent containers is respectively by separately solenoid control, and can under the effect of peristaltic pump 5, through respectively the Zeo-karb 6 that absorption sample to be tested, enter colorimetric pool behind the wash-out and carry out stratographic analysis.
The specific embodiment of eluent is as follows:
Embodiment one
Embodiment two
Embodiment three
The eluent of above-described embodiment is used for glycolated hemoglobin analysis, carries out four gradient elution analyses, its preci-sion and accuracy is tested, the result is as follows:
1. precision test
| Sequence number | Embodiment one | Embodiment two | Embodiment three |
| 1 | 9.85 | 9.80 | 9.75 |
| 2 | 9.80 | 9.58 | 9.77 |
| 3 | 9.76 | 9.70 | 9.49 |
| 4 | 9.74 | 9.86 | 9.76 |
| 5 | 9.56 | 9.76 | 9.80 |
| 6 | 9.79 | 9.76 | 9.64 |
| 7 | 9.56 | 9.59 | 9.59 |
| 8 | 9.76 | 9.76 | 9.55 |
| 9 | 9.46 | 9.64 | 9.65 |
| 10 | 9.75 | 9.66 | 9.63 |
| Average | 9.70 | 9.71 | 9.66 |
| Standard deviation | 0.13 | 0.09 | 0.10 |
| The coefficient of variation (%) | 1.32 | 0.95 | 1.07 |
2. accuracy test
| Sequence number | Embodiment one | Embodiment two | Embodiment three |
| 1 | 5.76 | 5.59 | 5.66 |
| 2 | 5.74 | 5.54 | 5.71 |
| 3 | 5.61 | 5.61 | 5.69 |
| Average | 5.70 | 5.58 | 5.69 |
| Quality-control product target value | 5.5 | 5.5 | 5.5 |
| Accuracy (%) | 0.2 | 0.08 | 0.19 |
Claims (1)
1. glycolated hemoglobin analysis four gradient elution analysis methods, it is characterized in that: the eluent that four kinds of fixed concentrations are set, make it have the Zeo-karb of sample to be tested to carry out wash-out to absorption respectively, and the liquid behind the wash-out carried out stratographic analysis, determine the content of wash-out composition, and then definite glycosylated hemoglobin concentration;
Described four kinds of eluents are respectively:
The A eluent is used for Zeo-karb cleaning, balance; Its composition and weight ratio number percent are: NaH
2PO
40.146～0.166%, Na
2HPO
40.035～0.055%, NaCl 0.022～0.024%, KCl 0.032～0.034%, NaN
30.019～0.021%, deionized water surplus;
The B eluent is used for other subfractions of wash-out glycosylated hemoglobin HbA
1a, HbA
1bIts composition and weight ratio number percent are: NaH
2PO
40.124～0.144%, Na
2HPO
40.043～0.063%, NaCl 0.341～0.361%, KCl 0.034～0.036%, NaN
30.019～0.021%, deionized water surplus;
The C eluent is used for wash-out glycosylated hemoglobin HbA
1cSubfraction; Its composition and weight ratio number percent are: NaH
2PO
40.118～0.138%, Na
2HPO
40.039～0.041%, NaCl 0.688～0.708%, KCl 0.072～0.074%, NaN
30.019～0.021%, deionized water surplus;
D eluent: be used for the non-glycosylated hemoglobin of wash-out and cation regenerant exchange resin; Its composition and weight ratio number percent are: NaH
2PO
40.1～0.12%, Na
2HPO
40.064～0.074%, NaCl 1.605～1.805%, NaN
30.019～0.021%, deionized water surplus.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103969375A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | An elution method for liquid chromatography and inspection equipment thereof |
| CN103969349A (en) * | 2013-01-29 | 2014-08-06 | 深圳普门科技有限公司 | A washing method of inspection equipment and the inspection equipment thereof |
| CN103254306B (en) * | 2013-04-15 | 2015-06-10 | 桂林英美特生物技术有限公司 | Purification process for glycosylated hemoglobin |
| CN103278574B (en) * | 2013-04-26 | 2014-12-10 | 首都医科大学附属北京朝阳医院 | Method for detecting hemoglobin by liquid chromatogram-triple tandem quadrupole mass spectrometer |
| CN104865326A (en) * | 2015-05-28 | 2015-08-26 | 江苏奥迪康医学科技有限公司 | Three-gradient elution analysis method of glycosylated hemoglobin analyzer |
| CN105597371B (en) * | 2016-01-14 | 2017-10-20 | 中国科学院过程工程研究所 | A kind of continuous gradient elution system and its processing method based on Simulation moving bed |
| CN107621550B (en) * | 2017-11-08 | 2024-05-07 | 济南佳宝乳业有限公司 | Food elution detects integration equipment |
| CN113009024B (en) * | 2018-12-29 | 2023-05-09 | 江山德瑞医疗科技有限公司 | Method for measuring elution condition in glycosylated hemoglobin |
| CN110441463A (en) * | 2019-07-04 | 2019-11-12 | 江苏奥迪康医学科技股份有限公司 | A kind of reagent of ion exchange chromatography detection Glycohemoglobin HbA1c |
| CN110887916A (en) * | 2019-12-21 | 2020-03-17 | 江苏奥迪康医学科技股份有限公司 | Reagent matched with glycosylated hemoglobin high-pressure liquid chromatography analyzer |
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Address after: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee after: JIANGSU AUDICOM MEDICAL TECHNOLOGY CO., LTD. Address before: 212009 Zhenjiang city Jiangsu province area Dingmao latitude four Road No. 36 high tech Industrial Park, No. 4 Building 4 layer Patentee before: Jiangsu Audicom Medical Technology Co., Ltd. |