CN109060992A - The glycolated hemoglobin analysis of detection accuracy can be improved - Google Patents
The glycolated hemoglobin analysis of detection accuracy can be improved Download PDFInfo
- Publication number
- CN109060992A CN109060992A CN201811016694.2A CN201811016694A CN109060992A CN 109060992 A CN109060992 A CN 109060992A CN 201811016694 A CN201811016694 A CN 201811016694A CN 109060992 A CN109060992 A CN 109060992A
- Authority
- CN
- China
- Prior art keywords
- valve
- pressure pump
- pump
- mobile phase
- direction changeover
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 108010054147 Hemoglobins Proteins 0.000 title claims abstract description 13
- 102000001554 Hemoglobins Human genes 0.000 title claims abstract description 13
- 238000001514 detection method Methods 0.000 title claims abstract description 10
- 238000005070 sampling Methods 0.000 claims description 16
- 238000003860 storage Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 239000002699 waste material Substances 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 238000009826 distribution Methods 0.000 abstract description 6
- 102000017011 Glycated Hemoglobin A Human genes 0.000 abstract description 4
- 108010014663 Glycated Hemoglobin A Proteins 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000001133 acceleration Effects 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000007872 degassing Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000002641 glycemic effect Effects 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/28—Control of physical parameters of the fluid carrier
- G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
Abstract
The present invention discloses a kind of glycolated hemoglobin analysis that detection accuracy can be improved, equipped with the high-pressure pump to connect respectively with the outlet of two mobile phases and the mixer to connect with two high pressure pump outlets, two mobile phase ratio distribution in the unit time are completed by the flow velocity of two high-pressure pumps of control, no setting is required degasser and proportion magnetic valve, not only simplify device structure, and the problems such as overcoming the inaccurate distribution of mobile phase ratio present in the prior art and poor repeatability, improve the accuracy of glycosylated hemoglobin analysis.
Description
Technical field
The present invention relates to a kind of glycolated hemoglobin analysis, especially a kind of structure is simple, detection accuracy can be improved
Glycolated hemoglobin analysis.
Background technique
Glycosylated hemoglobin is glucose and hemoglobin cross-linking products in blood, when the concentration of glucose in blood is higher
When, it is also just relatively high that human body is formed by saccharification hemoglobin content.Therefore, glycosylated hemoglobin as diabetes screening,
Diagnosis, glycemic control, efficacy assessment effective Testing index, be widely used in clinic.Existing HbAle egg
The principle of white analyzer is identical as high performance liquid chromatograph, and structure is provided with three-way switch valve, and three-way switch valve is respectively with
One syringe pump, the second syringe pump, first liang of direction changeover valve and sampling valve (six tunnel switching valves) connect, with first liang of direction changeover valve phase
It is connected to cleaning liquid storage tank and second liang of direction changeover valve, sampling valve is connected by two direction changeover valve of third with sample introduction needle；There are two if
Mobile phase storage tank, two mobile phase storage tanks pass sequentially through degasser, proportion magnetic valve and a high-pressure pump, and (double plunger is in parallel past
Multiple pump) connect, the output of high-pressure pump passes sequentially through pressure transmitter again, pot strainer connects with sampling valve, and sampling valve leads to again
Chromatographic column is crossed to connect with detector.Existing glycolated hemoglobin analysis is worked in high-pressure pump for mobile phase distribution method
Each channel opening time for the ratio control proportion magnetic valve that the unit time of journey sets according to mobile phase, there are the following problems:
1. due to double plunger and plunger rod that connection reciprocating pump convexity wheel drives is within the unit time and non-continuous imbibition, even if
During suction, the movement of plunger rod be also it is nonlinear, aspirate mobile phase speed will appear acceleration, at the uniform velocity with slow down three
Stage, therefore proportion magnetic valve each channel unlatching period appears in above-mentioned different phase, is in addition also influenced by the response time, nothing
Method guarantees the accuracy and repeatability of mobile phase ratio distribution；If the unlatching period of proportion magnetic valve is in parallel with double plunger reciprocal
Cam rotation period in pump is consistent, then can generate covibration, and then form larger noise, can equally reduce testing result
Accuracy.
2. mobile phase can generate biggish bubble in the handoff procedure of proportion magnetic valve, into can then be accounted in high-pressure pump
According to flowing phase space, mobile phase ratio is caused to distribute misalignment, therefore need that degasser is arranged before proportion magnetic valve, leads to equipment
Structure is relative complex.
Summary of the invention
The present invention is to solve above-mentioned technical problem present in the prior art, and providing one kind can be improved detection accuracy
Glycolated hemoglobin analysis.
The technical solution of the invention is as follows: a kind of glycolated hemoglobin analysis that detection accuracy can be improved is equipped with
Three-way switch valve, three-way switch valve connect with the first syringe pump, the second syringe pump, first liang of direction changeover valve and sampling valve respectively,
It is connected to cleaning liquid storage tank and second liang of direction changeover valve with first liang of direction changeover valve, the sampling valve passes through two direction changeover valve of third
Connecting with sample introduction needle, second liang of direction changeover valve connects with the first high-pressure pump and the second high pressure pump inlet respectively, and described first
The outlet of high-pressure pump and the second high-pressure pump is connected by waste collection control valve with waste liquid barrel；With the entrance also phase of the first high-pressure pump
Be connected to the first mobile phase storage tank, be also connected to the second mobile phase storage tank with the entrance of the second high-pressure pump, first high-pressure pump and
The outlet of second high-pressure pump also connects with mixer, the outlet of the mixer by pressure transmitter, pot strainer with into
Sample valve connects, and the sampling valve is connected by chromatographic column with detector.
The present invention is equipped with the high-pressure pump that connects respectively with the outlet of two mobile phases and connects with two high pressure pump outlets
Mixer completes two mobile phase ratio distribution in the unit time, degassing that no setting is required by the flow velocity of two high-pressure pumps of control
Device and proportion magnetic valve not only simplify device structure, and overcome the distribution of mobile phase ratio present in the prior art
The problems such as inaccurate and poor repeatability, improves the accuracy of glycosylated hemoglobin analysis.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the embodiment of the present invention.
Specific embodiment
The glycolated hemoglobin analysis that detection accuracy can be improved of the invention is equipped with three-way switch valve 1, threeway switching
Valve 1 connects with the first syringe pump 2,3, first liang of direction changeover valves 4 of the second syringe pump and sampling valve 5 respectively, the first syringe pump 2 and
Two syringe pumps, 3 capacity is different；Be connected to cleaning liquid storage tank 6 and second liang of direction changeover valve 7 with first liang of direction changeover valve 4, it is described into
Sample valve 5 is connected with sample introduction needle 9 by two direction changeover valve 8 of third, second liang of direction changeover valve 7 respectively with the first high-pressure pump 10 and
Second high-pressure pump, 11 entrance connects, and the outlet of first high-pressure pump 10 and the second high-pressure pump 11 passes through waste collection control valve 12
Connect with waste liquid barrel 13；It is also connected to the first mobile phase storage tank 14 with the entrance of the first high-pressure pump 10, with the second high-pressure pump 11
Entrance is also connected to the second mobile phase storage tank 15, the outlet of first high-pressure pump 10 and the second high-pressure pump 11 also with mixer 16
Connect, the outlet of the mixer 16 is connected by pressure transmitter 17, pot strainer 18 with sampling valve 5, the sampling valve 5
Connected by chromatographic column 19 with detector 20.
When detection, second liang of direction changeover valve 7 is closed, the first high-pressure pump 10 and the second high-pressure pump 11 extract the first flowing respectively
Mobile phase in phase storage tank 14 and the second mobile phase storage tank 15 controls the flow velocity of the first high-pressure pump 10 and the second high-pressure pump 11, i.e.,
The amount for entering two mobile phases of mixer in the control unit time is proportionally required, realizes the proportional assignment of the two.It
Afterwards by entering detector 20 after pressure transmitter 17, pot strainer 18, sampling valve 5, chromatographic column 19.It can be according to requiring to set
First syringe pump 2 or the work of the second syringe pump 3, extract blood sample by sample introduction needle 9, are mixed into color by sampling valve 5 and mobile phase
Column is composed, detector detection is entered back into.When needing to clean equipment, it can control first liang of direction changeover valve, 4, second liang of direction changeover valves 7
And two direction changeover valve 8 of third, cleaning solution is extracted by the first syringe pump 2 or the second syringe pump 3 and cleans sample introduction needle 9, by the first high pressure
Pump 10 and the second high-pressure pump 11 extract cleaning solution respectively and clean to the other parts of glycolated hemoglobin analysis, open useless
Liquid collects control valve 12, collects waste liquid by waste liquid barrel 13.
Claims (1)
1. a kind of glycolated hemoglobin analysis that detection accuracy can be improved is equipped with three-way switch valve (1), three-way switch valve
(1) connect respectively with the first syringe pump (2), the second syringe pump (3), first liang of direction changeover valve (4) and sampling valve (5), with first
Two direction changeover valves (4) are connected to cleaning liquid storage tank (6) and second liang of direction changeover valve (7), and the sampling valve (5) is logical by third two
Switching valve (8) connects with sample introduction needle (9), it is characterised in that: second liang of direction changeover valve (7) respectively with the first high-pressure pump (10)
And second high-pressure pump (11) entrance connect, the outlet of first high-pressure pump (10) and the second high-pressure pump (11) passes through waste collection
Control valve (12) connects with waste liquid barrel (13)；It is also connected to the first mobile phase storage tank (14) with the entrance of the first high-pressure pump (10),
The second mobile phase storage tank (15), first high-pressure pump (10) and the second high pressure are also connected to the entrance of the second high-pressure pump (11)
The outlet of pump (11) also connects with mixer (16), and the outlet of the mixer (16) passes through pressure transmitter (17), online mistake
Filter (18) connects with sampling valve (5), and the sampling valve (5) is connected by chromatographic column (19) with detector (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811016694.2A CN109060992B (en) | 2018-09-03 | 2018-09-03 | Glycosylated hemoglobin analyzer capable of improving detection accuracy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811016694.2A CN109060992B (en) | 2018-09-03 | 2018-09-03 | Glycosylated hemoglobin analyzer capable of improving detection accuracy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109060992A true CN109060992A (en) | 2018-12-21 |
| CN109060992B CN109060992B (en) | 2024-02-27 |
Family
ID=64759034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811016694.2A Active CN109060992B (en) | 2018-09-03 | 2018-09-03 | Glycosylated hemoglobin analyzer capable of improving detection accuracy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109060992B (en) |
Citations (17)
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|---|---|---|---|---|
| US5071547A (en) * | 1990-03-23 | 1991-12-10 | Separations Technology, Inc. | Column chromatographic column apparatus with switching capability |
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| JP2000028598A (en) * | 1998-07-14 | 2000-01-28 | Ebara Corp | Full automatic refining, analyzing device, method for continuously refining, and analyzing sample |
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| CN107064379A (en) * | 2017-03-10 | 2017-08-18 | 福州大学 | A kind of online trace analysis methods of non-protein amino acid toxin |
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| CN208705287U (en) * | 2018-09-03 | 2019-04-05 | 大连技嘉科技有限公司 | The glycolated hemoglobin analysis of detection accuracy can be improved |
-
2018
- 2018-09-03 CN CN201811016694.2A patent/CN109060992B/en active Active
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5071547A (en) * | 1990-03-23 | 1991-12-10 | Separations Technology, Inc. | Column chromatographic column apparatus with switching capability |
| WO1993007168A2 (en) * | 1991-09-30 | 1993-04-15 | Perseptive Biosystems, Inc. | Protein chromatography system |
| JPH09274029A (en) * | 1996-04-04 | 1997-10-21 | Tosoh Corp | Saccharized hemoglobin analyzer |
| JP2000028598A (en) * | 1998-07-14 | 2000-01-28 | Ebara Corp | Full automatic refining, analyzing device, method for continuously refining, and analyzing sample |
| EP1154270A2 (en) * | 2000-05-09 | 2001-11-14 | Tosoh Corporation | Glycated hemoglobin analyzer |
| JP2002031626A (en) * | 2000-05-09 | 2002-01-31 | Tosoh Corp | Saccharified hemoglobin analyzer |
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| Publication number | Publication date |
|---|---|
| CN109060992B (en) | 2024-02-27 |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20200605 Address after: 215011 building 2-2-211, No.2, Peiyuan Road, high tech Zone, Suzhou City, Jiangsu Province Applicant after: Suzhou Borui Jiasheng Medical Technology Co.,Ltd. Address before: 116000 north side of No. 20, AI Xian street, hi tech Industrial Park, Dalian, Liaoning. Applicant before: DALIAN JIJIA TECHNOLOGY Co.,Ltd. |
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