CN203688437U - Chromatographic column effluent detection device - Google Patents
Chromatographic column effluent detection device Download PDFInfo
- Publication number
- CN203688437U CN203688437U CN201320789912.2U CN201320789912U CN203688437U CN 203688437 U CN203688437 U CN 203688437U CN 201320789912 U CN201320789912 U CN 201320789912U CN 203688437 U CN203688437 U CN 203688437U
- Authority
- CN
- China
- Prior art keywords
- chromatographic column
- detection device
- protein detection
- protein
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000002331 protein detection Methods 0.000 claims abstract description 26
- 239000002699 waste material Substances 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 238000001742 protein purification Methods 0.000 abstract description 4
- 238000000746 purification Methods 0.000 abstract description 2
- 238000009776 industrial production Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 8
- 238000011161 development Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- -1 biology Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000011897 real-time detection Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Images
Landscapes
- Treatment Of Liquids With Adsorbents In General (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model provides a chromatographic column effluent detection device, belonging to the field of biological purification devices. In the chromatographic column effluent detection device provided by the utility model, a valve is arranged between a sample collecting container and a chromatographic column as well as between a waste liquor collecting container and the chromatographic column respectively, so that effluent produced during protein purification can be monitored in an entire industrial production process, a protein detection instrument is prevented from being damaged, and waste of proteins flowing out of the protein detection instrument is avoided.
Description
Technical field
The utility model belongs to biologically pure device, is specifically related to a kind of chromatographic column effluent liquid pick-up unit.
Background technology
At present, the biochemical field development of China is swift and violent, directly drive the development of field of medicaments, constantly development, the medicine of producing and the technology of research and development have improved national medical condition, current diversified biology, medical purification production technique reach its maturity, and entirety has improved the molecular engineering of China in the application level of medicine, biology, chemical field.
Protein is as the requisite component of composition human body, be described as the living matter of human body, natural protein supplementing and absorbing mainly from animal meat and cereal etc. as human body, for extract protein from refuse, become biochemical field and produced a large trend of protein, protein is expensive also strict to the mistake range request of its production of purifying because of it, and output is extremely low, more and more can not meet the demand in market.
Current protein purification system is generally only applicable to the technical study operation in laboratory; And the chromatographic column of existing industrial application is in chromatography process, there is a difficult problem for complete monitoring.Former because: when industry's enlarging production, chromatographic column flow velocity is excessive, therefore needs to be equipped with to have the detecting device in large flow rate pond, causes equipment cost high; If when excessive flow velocity passes through checkout equipment, also easily checkout equipment is caused damage.
Summary of the invention
For addressing the above problem, the utility model provides a kind of chromatographic column effluent liquid pick-up unit, adopts following technical scheme:
A kind of chromatographic column effluent liquid pick-up unit, comprise chromatographic column, collection containers, Protein Detection device and waste collection container, it is characterized in that, the outlet of described chromatographic column connects four-way piece, other three ends of described four-way piece are connected with collection containers, Protein Detection device and waste collection container by valve respectively, the endpiece of described Protein Detection device is connected with three-way pipe, and two outer two ends of described three-way pipe are connected with collection containers and waste collection container by valve respectively.
Preferably, valve recited above all adopts solenoid valve.
The beneficial effects of the utility model are as follows:
Traditional chromatographic column effluent liquid pick-up unit, the efflux of chromatographic column first enters collection containers or waste collection container by Protein Detection device completely later again, and the chromatographic column effluent liquid pick-up unit that the utility model provides, outlet at chromatographic column connects a four-way piece, and other three ends of four-way piece are respectively by valve and collection containers, Protein Detection device is connected with waste collection container, the benefit arranging is like this: the size of opening by control valve, thereby regulate flow into amount of liquid in Protein Detection device number, realize a small amount of liquid is flowed in Protein Detection device, the sample ultraviolet absorption value in liquid is changed into electric signal by described Protein Detection device, connect computer, can show on computers the variation of sample in efflux, realize visual, by the sample message representing in Protein Detection device, regulate the valve of collection containers and waste collection container top, sample or waste liquid are collected.Therefore, the pick-up unit that the utility model provides, the efflux in can producing industrialized protein purification carries out complete monitoring, and not fragile Protein Detection instrument, also can not waste the albumen flowing out from Protein Detection instrument.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model; Wherein, 1 represents collection containers; 2 represent waste collection container; 3 represent Protein Detection device; 4 represent chromatographic column; 5 represent four-way piece; 6 represent three-way pipe.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.Except following embodiment, the utility model can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of the utility model requirement.
As shown in Figure 1, a kind of chromatographic column effluent liquid pick-up unit, comprise chromatographic column 4, collection containers 1, Protein Detection device 3 and waste collection container 2, it is characterized in that, the outlet of described chromatographic column connects four-way piece 5, other three ends of described four-way piece are connected with collection containers 1, Protein Detection device 3 and waste collection container 2 by valve respectively, the endpiece of described Protein Detection device 3 is connected with three-way pipe 6, and two outer two ends of described three-way pipe 6 are connected with collection containers 1 and waste collection container 2 by valve respectively.
Preferably, valve recited above all adopts solenoid valve.
Principle of work of the present utility model is as follows:
If the liquid flowing out from chromatographic column contains sample, open and connect collection containers and the valve that is connected Protein Detection device, a small amount of sample solution enters Protein Detection device, and most sample solution enters collection containers, the solution that flows process from Protein Detection device also enters in collection containers, does not cause the waste that detects solution.
The sample ultraviolet absorption value in liquid is changed into electric signal by Protein Detection device, connects computer, can show on computers the variation of sample in efflux, realizes visual.So, when protein purification routine change, can find out easily the variation of sample size in Protein Detection device, if the protein content showing in detecting device is very low or while not having, close the valve connecting in collection containers, open the valve that connects waste collection container, now, still can keep a small amount of sample solution to enter Protein Detection device and proceed real-time detection, most waste liquid enters in waste collection container.
Claims (2)
1. a chromatographic column effluent liquid pick-up unit, comprise chromatographic column, collection containers, Protein Detection device and waste collection container, it is characterized in that, the outlet of described chromatographic column connects four-way piece, other three ends of described four-way piece are connected with collection containers, Protein Detection device and waste collection container by valve respectively, the endpiece of described Protein Detection device is connected with three-way pipe, and two outer two ends of described three-way pipe are connected with collection containers and waste collection container by valve respectively.
2. chromatographic column effluent liquid pick-up unit according to claim 1, is characterized in that, described valve is solenoid valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789912.2U CN203688437U (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320789912.2U CN203688437U (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203688437U true CN203688437U (en) | 2014-07-02 |
Family
ID=51010324
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320789912.2U Expired - Lifetime CN203688437U (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203688437U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698289A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatographic column effluent detection device |
| CN114700018A (en) * | 2022-04-08 | 2022-07-05 | 上海交通大学 | Semi-automatic integrated reaction device and preparation method of imidazole amino acid ionic liquid |
-
2013
- 2013-12-05 CN CN201320789912.2U patent/CN203688437U/en not_active Expired - Lifetime
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103698289A (en) * | 2013-12-05 | 2014-04-02 | 成都雅途生物技术有限公司 | Chromatographic column effluent detection device |
| CN114700018A (en) * | 2022-04-08 | 2022-07-05 | 上海交通大学 | Semi-automatic integrated reaction device and preparation method of imidazole amino acid ionic liquid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20140702 |