CN103698289A - Chromatographic column effluent detection device - Google Patents
Chromatographic column effluent detection device Download PDFInfo
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- CN103698289A CN103698289A CN201310644053.2A CN201310644053A CN103698289A CN 103698289 A CN103698289 A CN 103698289A CN 201310644053 A CN201310644053 A CN 201310644053A CN 103698289 A CN103698289 A CN 103698289A
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- chromatographic column
- detection device
- protein
- protein detection
- collection container
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- Treatment Of Liquids With Adsorbents In General (AREA)
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Abstract
The invention provides a chromatographic column effluent detection device, belonging to the field of biological purification devices. According to the chromatographic column effluent detection device provided by the invention, two valves are respectively arranged between a sample collection container and a chromatographic column, and between a waste liquid collection container and the chromatographic column. The chromatographic column effluent detection device can perform full-process monitoring on effluent during protein purification in industrial production, is less prone to damage a protein detection instrument, and can prevent the waste of protein flowing out of the protein detection instrument.
Description
Technical field
The invention belongs to biologically pure device, be specifically related to a kind of chromatographic column effluent liquid pick-up unit.
Background technology
At present, the biochemical field development of China is swift and violent, directly driven the development of field of medicaments, constantly development, the medicine of producing and the technology of research and development have improved national medical condition, current diversified biology, medical purification production technique reach its maturity, and integral body has improved the molecular engineering of China in the application level of medicine, biology, chemical field.
Protein is as forming the requisite component of human body, be described as the living matter of human body, natural protein supplementing and absorbing mainly from animal meat and cereal etc. as human body, for extract protein from refuse, become biochemical field and produced a large trend of protein, protein is expensive also strict to the mistake range request of its production of purifying because of it, and output is extremely low, more and more can not meet the demand in market.
Current protein purification system is generally only applicable to the technical study operation in laboratory; And the chromatographic column of existing industrial application is in chromatography process, there is a difficult problem for complete monitoring.Former because: therefore during industry's enlarging production, chromatographic column flow velocity is excessive, needs to be equipped with the detecting device with large flow rate pond, causes equipment cost high; If when excessive flow velocity passes through checkout equipment, also easily checkout equipment is caused damage.
Summary of the invention
For addressing the above problem, the invention provides a kind of chromatographic column effluent liquid pick-up unit, adopt following technical scheme:
A kind of chromatographic column effluent liquid pick-up unit, comprise chromatographic column, collection containers, Protein Detection device and waste collection container, it is characterized in that, the outlet of described chromatographic column connects four-way piece, other three ends of described four-way piece are connected with collection containers, Protein Detection device and waste collection container by valve respectively, the endpiece of described Protein Detection device is connected with three-way pipe, and two outer two ends of described three-way pipe are connected with waste collection container with collection containers by valve respectively.
Preferably, valve recited above all adopts solenoid valve.
Beneficial effect of the present invention is as follows:
Traditional chromatographic column effluent liquid pick-up unit, the efflux of chromatographic column first enters collection containers or waste collection container by Protein Detection device completely later again, and chromatographic column effluent liquid pick-up unit provided by the invention, outlet at chromatographic column connects a four-way piece, and other three ends of four-way piece are respectively by valve and collection containers, Protein Detection device is connected with waste collection container, the benefit arranging is like this: the size of opening by control valve, thereby regulate to flow into amount of liquid in Protein Detection device number, realization flows into a small amount of liquid in Protein Detection device, described Protein Detection device changes into electric signal by the sample ultraviolet absorption value in liquid, connect computer, can show on computers the variation of sample in efflux, realize visual, by the sample message representing in Protein Detection device, regulate the valve of collection containers and waste collection container top, sample or waste liquid are collected.Therefore, pick-up unit provided by the invention, the efflux in can producing industrialized protein purification carries out complete monitoring, and not fragile Protein Detection instrument, also can not waste the albumen flowing out from Protein Detection instrument.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention; Wherein, 1 represents collection containers; 2 represent waste collection container; 3 represent Protein Detection device; 4 represent chromatographic column; 5 represent four-way piece; 6 represent three-way pipe.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.Except following embodiment, the present invention can also have other embodiments, and all employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection domain of requirement of the present invention.
As shown in Figure 1, a kind of chromatographic column effluent liquid pick-up unit, comprise chromatographic column 4, collection containers 1, Protein Detection device 3 and waste collection container 2, it is characterized in that, the outlet of described chromatographic column connects four-way piece 5, other three ends of described four-way piece are connected with collection containers 1, Protein Detection device 3 and waste collection container 2 by valve respectively, the endpiece of described Protein Detection device 3 is connected with three-way pipe 6, and two outer two ends of described three-way pipe 6 are connected with waste collection container 2 with collection containers 1 by valve respectively.
Preferably, valve recited above all adopts solenoid valve.
Principle of work of the present invention is as follows:
If the liquid flowing out from chromatographic column contains sample, open and connect collection containers and the valve that is connected Protein Detection device, a small amount of sample solution enters Protein Detection device, and most sample solution enters collection containers, the solution that flows process from Protein Detection device also enters in collection containers, does not cause the waste that detects solution.
Protein Detection device changes into electric signal by the sample ultraviolet absorption value in liquid, connects computer, can show on computers the variation of sample in efflux, realizes visual.So, during protein purification routine change, can find out easily the variation of sample size in Protein Detection device, if the protein content showing in detecting device is very low or while not having, close the valve connecting in collection containers, open the valve that connects waste collection container, now, still can keep a small amount of sample solution to enter Protein Detection device and proceed real-time detection, most waste liquid enters in waste collection container.
Claims (2)
1. a chromatographic column effluent liquid pick-up unit, comprise chromatographic column, collection containers, Protein Detection device and waste collection container, it is characterized in that, the outlet of described chromatographic column connects four-way piece, other three ends of described four-way piece are connected with collection containers, Protein Detection device and waste collection container by valve respectively, the endpiece of described Protein Detection device is connected with three-way pipe, and two outer two ends of described three-way pipe are connected with waste collection container with collection containers by valve respectively.
2. chromatographic column effluent liquid pick-up unit according to claim 1, is characterized in that, described valve is solenoid valve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644053.2A CN103698289A (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310644053.2A CN103698289A (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103698289A true CN103698289A (en) | 2014-04-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310644053.2A Pending CN103698289A (en) | 2013-12-05 | 2013-12-05 | Chromatographic column effluent detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965277A (en) * | 2020-08-06 | 2020-11-20 | 天津科德通生物科技有限公司 | Automatic collection device for antibody purification effluent liquid |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070109088A (en) * | 2006-05-09 | 2007-11-15 | 연세대학교 산학협력단 | - An Apparatus for Protein Separation Using Capillary Isoelectric Focusing-Hollow Fiber Flow Field Flow Fractionation and Method Thereof |
| CN201161989Y (en) * | 2007-08-14 | 2008-12-10 | 百奇生物科技(上海)有限公司 | Multicenter protein compatible chromatography purification instrument |
| CN102258885A (en) * | 2011-05-18 | 2011-11-30 | 中国地质大学（武汉） | Automatic separation system for integrated column chromatography |
| CN103394211A (en) * | 2013-08-21 | 2013-11-20 | 利穗科技(苏州)有限公司 | Apparatus for rapidly separating and purifying organic compounds and separation and purification method |
| CN203688437U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Chromatographic column effluent detection device |
-
2013
- 2013-12-05 CN CN201310644053.2A patent/CN103698289A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070109088A (en) * | 2006-05-09 | 2007-11-15 | 연세대학교 산학협력단 | - An Apparatus for Protein Separation Using Capillary Isoelectric Focusing-Hollow Fiber Flow Field Flow Fractionation and Method Thereof |
| CN201161989Y (en) * | 2007-08-14 | 2008-12-10 | 百奇生物科技(上海)有限公司 | Multicenter protein compatible chromatography purification instrument |
| CN102258885A (en) * | 2011-05-18 | 2011-11-30 | 中国地质大学（武汉） | Automatic separation system for integrated column chromatography |
| CN103394211A (en) * | 2013-08-21 | 2013-11-20 | 利穗科技(苏州)有限公司 | Apparatus for rapidly separating and purifying organic compounds and separation and purification method |
| CN203688437U (en) * | 2013-12-05 | 2014-07-02 | 成都雅途生物技术有限公司 | Chromatographic column effluent detection device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111965277A (en) * | 2020-08-06 | 2020-11-20 | 天津科德通生物科技有限公司 | Automatic collection device for antibody purification effluent liquid |
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Application publication date: 20140402 |
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