CN204151270U - A kind of industrial preparation scale protein separation system - Google Patents
A kind of industrial preparation scale protein separation system Download PDFInfo
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- CN204151270U CN204151270U CN201420633503.8U CN201420633503U CN204151270U CN 204151270 U CN204151270 U CN 204151270U CN 201420633503 U CN201420633503 U CN 201420633503U CN 204151270 U CN204151270 U CN 204151270U
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Abstract
The utility model relates to a kind of industrial preparation scale protein separation system, comprises computer (1), electrical control cabinet (2), solvent valve group (3), sampling valve group (4), air bubble sensor (5), infusion pump (6), under meter (7), air-vent valve group (8), air-vent (9), pressure transmitter (10), pH detector (11), conductivity detector (12), post position switching valve group (13), chromatographic column (14), UV-detector (15), conductivity detector (16) and fraction collection valve group (17).The utility model can complete the separation and purification process of protein automatically, level of automation is high, can on-line monitoring pH value, conductivity value and ultraviolet absorption value after conductivity value, chromatographic column before chromatographic column, these experiment parameters can ensure quality and the purity of proteinaceous product after separation and purification, adopt the infusion pump of large flux and the design of large diameter chromatographic column simultaneously, can greatly improve the output of an experimental protein separation and purification process.
Description
Technical field
The utility model relates to a kind of industrial preparation scale protein separation system, belongs to liquid chromatography field.
Background technology
Protein (comprises catalyze metabolic reaction, substance operation, excited conduction, the control etc. of growing) and plays an important role in the vital movement process of Cell and organism body, is the very important research object of life science.And the separation and purification of protein is link indispensable in protein research, along with international and domestic market is to the increase day by day of its demand, also there is great demand to its advanced isolation technique.
As the mainstream technology of protein separation, column chromatography has very high separation efficiency, usually adopts fixed-bed intermittent operating method, simple to operate, but there is the problems such as level of automation is low, function singleness, carrier utilization ratio is low, process efficiency is low.Can not meet high yield in industrial production, high-level efficiency and highly purified requirement far away.
Summary of the invention
For solving the problem, the utility model provides a kind of industrial preparation scale protein separation system, adopt the separation and purification process of the whole protein of computer control system overall monitor, ensure quality and the purity of protein separation, adopt large flux to design the satisfied demand to protein separation output simultaneously.
A kind of industrial preparation scale protein separation system, comprises computer 1, electrical control cabinet 2, solvent valve group 3, sampling valve group 4, air bubble sensor 5, infusion pump 6, under meter 7, air-vent valve group 8, air-vent 9, pressure transmitter 10, pH detector 11, conductivity detector 12, post position switching valve group 13, chromatographic column 14, UV-detector 15, conductivity detector 16, fraction collection valve group 17.
Computer 1 is industrial control computer; Electrical control cabinet 2 is positive voltage type anti-explosion electrical control cabinet or common electrical housing, and the controller in regulator cubicle adopts PLC; Solvent valve group 3 is multiple valve groups, and the quantity of valve is 1 ~ 10, and each valve can automatically switch and select different solvents; Sampling valve group 4 is multiple valve groups, and the quantity of valve is 1 ~ 10, and each valve can automatically switch and select different samples, to avoid the crossed contamination of sample; Whether air bubble sensor 5 is used for online monitoring system pipeline has bubble; Infusion pump 6 is ram pump or surge pump, is used for transport flow phase solvent and sample, and flow range is 10 ~ 3000L/h; Under meter 7 is Coriolis flowmeter or magnetic flow meter, is used for the flow of on-line measurement system; Air-vent valve group 8 is three track valve groups, can switch as required liquid in pipeline through or without air-vent; The device that air-vent 9 is used to bubble in elimination system avoids bubble to enter in chromatographic column; Pressure transmitter 10 is used for the pressure of online monitoring system; PH detector 11 is used for the pH value of online monitoring system; The conductivity value of moving phase before conductivity detector 12 on-line monitoring chromatographic column; Post position switching valve group 13 is four-way valve groups, can switch the flow direction of moving phase as required, carry out square impact or backflush operation to chromatographic column; Chromatographic column 14 is glass column or stainless steel chromatographic column, and diameter range is 100 ~ 1500mm; UV-detector 15 is ultraviolet-visible variable-wavelenght detector or fixed wave length detector, with optical fiber interface; The conductivity value of flowing liquid after conductivity detector 16 on-line monitoring chromatographic column; Fraction collection valve group 17 multiple valve group, the quantity of valve is 1 ~ 15, and each valve can automatically switch and carry out the collection of different fractions.
The beneficial effects of the utility model are: (1) adopts the Design of Automatic Control System based on PLC, and automatically can complete the separation and purification process of protein, level of automation is high, can save a large amount of manpower; (2) by the kinds of experiments such as conductivity value and ultraviolet absorption value parameter after conductivity value, chromatographic column before the pH value of on-line detecting system, chromatographic column, quality and the purity of product is ensured; (3) adopt the infusion pump of large flux and the design of large diameter chromatographic column, the yield values of single protein separation can be improved.
Accompanying drawing explanation
Fig. 1 is frontview of the present utility model
Fig. 2 is rear view of the present utility model
Fig. 3 is right view of the present utility model
In figure, concrete digital code is as follows: 1, computer 2, electrical control cabinet 3, solvent valve group 4, sampling valve group 5, air bubble sensor 6, infusion pump 7, under meter 8, air-vent valve group 9, air-vent 10, pressure transmitter 11, pH detector 12, conductivity detector 13, post position switching valve group 14, chromatographic column 15, UV-detector 16, conductivity detector 17, fraction collection valve group.
Embodiment
The utility model provides a kind of industrial preparation scale protein separation system, comprises computer 1, electrical control cabinet 2, solvent valve group 3, sampling valve group 4, air bubble sensor 5, infusion pump 6, under meter 7, air-vent valve group 8, air-vent 9, pressure transmitter 10, pH detector 11, conductivity detector 12, post position switching valve group 13, chromatographic column 14, UV-detector 15 conductivity detector 16, fraction collection valve group 17.Computer 1 is arranged on electrical control cabinet 2; Influidic pipe connects solvent valve group 3, sampling valve group 4, infusion pump 6, under meter 7, air-vent valve group 8, air-vent 9, pressure transmitter 10, pH detector 11, conductivity detector 12, post position switching valve group 13, chromatographic column 14, UV-detector 15, conductivity detector 16, fraction collection valve group 17 successively.Air bubble sensor 5 is arranged on the influidic pipe between solvent valve group 3 and infusion pump 6.The signal wire of pressure transmitter 10, pH detector 11, conductivity detector 12, conductivity detector 16 is connected in the PLC in electrical control cabinet 2.
The utility model can complete the process such as loading, solvent elution, system balancing in protein separation process automatically, introduces working process of the present utility model below for loading and solvent elution process.
Loading process of the present utility model is: sample, by sampling valve group 4, is delivered under meter 7 by infusion pump 6 and detects, and change over signal after on the PLC that the flow signal of output reaches regulator cubicle 2, is shown by computer 1; In order to reduce sample diffusion, sample directly enters pressure transmitter 10 after air-vent valve group 8, and after pressure transmitter 10 on-line detecting system pressure, output pressure signal carries out signal conversion to the PLC of regulator cubicle 2, is shown by computer 1; Sample enters the flow-through cell of pH detector 11 and conductivity detector 12, the pH detected and conductivity value are exported to conversion on the PLC of regulator cubicle 2 and are shown by computer 1 by pH detector 11 and conductivity detector 12, and sample enters chromatographic column 14 through post position switching valve group 13 again and completes loading.
Solvent elution process of the present utility model is: solvent, by solvent valve group 3, is delivered under meter 7 by infusion pump 6 and detects, and change over signal after on the PLC that the flow signal of output reaches regulator cubicle 2, is shown by computer 1, whether air bubble sensor 5 detects in solvent containing bubble, if have, solvent then enters air-vent 9 by air-vent valve group 8, air-vent 9 enters pressure transmitter 10 after eliminating the bubble in solvent, if nothing, solvent directly enters pressure transmitter 10 through air-vent valve group 8, and after pressure transmitter 10 on-line detecting system pressure, output pressure signal carries out signal conversion to the PLC of regulator cubicle 2, is shown by computer 1, solvent enters the flow-through cell of pH detector 11 and conductivity detector 12, the pH detected and conductivity value are exported to conversion on the PLC of regulator cubicle 2 and are shown by computer 1 by pH detector 11 and conductivity detector 12, solvent enters chromatographic column 14 after post position switching valve group 13, a series of chemical reaction is there is and by sample separation in chromatographic column 14, after the liquid flowed out enters UV-detector 15 and conductivity detector 16 detection, the ultraviolet absorption value exported and conductivity value signal reach the PLC upconverted signal of regulator cubicle 2, shown by computer 1, PLC controls the appointment valve opening of fraction collection valve group 16 according to ultraviolet absorption value and conductivity value simultaneously, liquid flows out through the valve of fraction collection valve group 16.
Claims (4)
1. an industrial preparation scale protein separation system, comprises computer (1), electrical control cabinet (2), solvent valve group (3), sampling valve group (4), air bubble sensor (5), infusion pump (6), under meter (7), air-vent valve group (8), air-vent (9), pressure transmitter (10), pH detector (11), conductivity detector (12), post position switching valve group (13), chromatographic column (14), UV-detector (15), conductivity detector (16) and fraction collection valve group (17), computer (1) is arranged on electrical control cabinet (2), influidic pipe connects solvent valve group (3) successively, sampling valve group (4), infusion pump (6), under meter (7), air-vent valve group (8), air-vent (9), pressure transmitter (10), pH detector (11), conductivity detector (12), post position switching valve group (13), chromatographic column (14), UV-detector (15), conductivity detector (16), fraction collection valve group (17), air bubble sensor (5) is arranged on the influidic pipe between solvent valve group (3) and infusion pump (6), pressure transmitter (10), pH detector (11), conductivity detector (12), the signal wire of conductivity detector (16) is connected in the PLC in electrical control cabinet (2).
2. one according to claim 1 industrial preparation scale protein separation system, is characterized in that: infusion pump (6) is ram pump or surge pump, and the quantitative range of pump is 1 ~ 4, and flow range is 10 ~ 3000L/h.
3. one according to claim 1 industrial preparation scale protein separation system, is characterized in that: flow counts Coriolis flowmeter or magnetic flow meter.
4. one according to claim 1 industrial preparation scale protein separation system, is characterized in that: UV-detector, with optical fiber interface, can be ultraviolet-visible variable-wavelenght detector, also can be fixed wave length detector.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420633503.8U CN204151270U (en) | 2014-10-30 | 2014-10-30 | A kind of industrial preparation scale protein separation system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420633503.8U CN204151270U (en) | 2014-10-30 | 2014-10-30 | A kind of industrial preparation scale protein separation system |
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| CN204151270U true CN204151270U (en) | 2015-02-11 |
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| CN201420633503.8U Active CN204151270U (en) | 2014-10-30 | 2014-10-30 | A kind of industrial preparation scale protein separation system |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105194905A (en) * | 2015-10-22 | 2015-12-30 | 宁波荣安生物药业有限公司 | Chromatography system |
| CN106749503A (en) * | 2017-03-10 | 2017-05-31 | 湖南铭沃生物科技有限公司 | A kind of integral type protein purification chromatographic apparatus |
| CN109634164A (en) * | 2018-11-09 | 2019-04-16 | 苏州赛谱仪器有限公司 | Protein chromatographic control system |
| CN109669033A (en) * | 2017-10-17 | 2019-04-23 | 普瑞邦（北京）科技有限公司 | A kind of immune affinity column operating device |
| CN110790813A (en) * | 2019-11-19 | 2020-02-14 | 上海多宁生物科技有限公司 | Multi-channel protein purification instrument |
| CN114797470A (en) * | 2022-05-05 | 2022-07-29 | 苏州赛谱仪器有限公司 | Protein purification refrigerating system |
-
2014
- 2014-10-30 CN CN201420633503.8U patent/CN204151270U/en active Active
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105194905A (en) * | 2015-10-22 | 2015-12-30 | 宁波荣安生物药业有限公司 | Chromatography system |
| CN105194905B (en) * | 2015-10-22 | 2017-04-12 | 宁波荣安生物药业有限公司 | Chromatography system |
| CN106749503A (en) * | 2017-03-10 | 2017-05-31 | 湖南铭沃生物科技有限公司 | A kind of integral type protein purification chromatographic apparatus |
| CN109669033A (en) * | 2017-10-17 | 2019-04-23 | 普瑞邦（北京）科技有限公司 | A kind of immune affinity column operating device |
| CN109634164A (en) * | 2018-11-09 | 2019-04-16 | 苏州赛谱仪器有限公司 | Protein chromatographic control system |
| CN110790813A (en) * | 2019-11-19 | 2020-02-14 | 上海多宁生物科技有限公司 | Multi-channel protein purification instrument |
| CN114797470A (en) * | 2022-05-05 | 2022-07-29 | 苏州赛谱仪器有限公司 | Protein purification refrigerating system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 223005 No. 1-9, Jixian Road, Huai'an Economic Development Zone, Jiangsu Province Patentee after: Jiangsu Hanbang Technology Co.,Ltd. Address before: 223005 No. 1-9, Jixian Road, Huai'an Economic Development Zone, Jiangsu Province Patentee before: JIANGSU HANBON SCIENCE & TECHNOLOGY Co.,Ltd. |
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| CP01 | Change in the name or title of a patent holder |