CN207816912U - A kind of laboratory separation and purification of protein system - Google Patents
A kind of laboratory separation and purification of protein system Download PDFInfo
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- CN207816912U CN207816912U CN201721812349.0U CN201721812349U CN207816912U CN 207816912 U CN207816912 U CN 207816912U CN 201721812349 U CN201721812349 U CN 201721812349U CN 207816912 U CN207816912 U CN 207816912U
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- valve
- pressure sensor
- pipeline
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- detector
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Abstract
The utility model discloses a kind of laboratory separation and purification of protein systems, including entrance selector valve A, pressure sensor A, infusion pump A, entrance selector valve B, pressure sensor B, infusion pump B, sample inlet selector valve C, pressure sensor C, sample pump C, three-way connection, mixer, sampling valve, column position switching valve A, chromatographic column, column position switching valve B, pH detector, conductivity detector, UV detector, Fraction collection valve, system chassis.Whole system uses integrated integrated design, profit to computerized control separation and purification of protein process, high degree of automation, can monitor quality and purity that the kinds of processes parameter such as pH value, conductivity value and ultraviolet absorption value ensures protein purification on-line.
Description
Technical field
The utility model is related to a kind of laboratory separation and purification of protein systems, belong to chromatographic field.
Background technology
Protein is during the vital movement of cell and organism（Including catalysis metabolic response, substance operation, emerging
The control etc. of the conduction, growth and development put forth energy）It plays an important role, is the particularly important research object of life science.And egg
Isolating and purifying for white matter is link indispensable in protein research, with international and domestic market to its demand increasingly
Increase, also has great demand to its advanced isolation technics.As the mainstream technology of protein separation, column chromatography has
Very high separative efficiency, generally use fixed-bed intermittent mode of operation is easy to operate, but there are the degree of automation low, function list
One, the problems such as carrier utilization rate is low, process efficiency is low.
Invention content
To solve the above problems, the utility model provides a kind of laboratory separation and purification of protein system, whole system is adopted
With integrated integrated design, profit is computerizedd control separation and purification of protein process, ensures the quality and purity of separation and purification of protein.
A kind of laboratory separation and purification of protein system includes entrance selector valve A1, pressure sensor A2, infusion pump A3, entrance
Selector valve B4, pressure sensor B5, infusion pump B6, sample inlet selector valve C7, pressure sensor C8, sample pump C9, three connect
First 10, mixer 11, sampling valve 12, column position switching valve A13, chromatographic column 14, column position switching valve B 15, pH detectors 16, conductance
Rate detector 17, UV detector 18, Fraction collection valve 19, system chassis 20；Wherein, entrance selector valve A1 by pipeline with it is defeated
Liquid pump A3 is connected, and infusion pump A3 is connected by pipeline with pressure sensor A2, and entrance selector valve B4 passes through pipeline and infusion
Pump B6 is connected, and infusion pump B6 is connected by pipeline with pressure sensor B5, and pressure sensor A2 and pressure sensor B5 are logical
Pipeline access three-way connection 10 is crossed, three-way connection 10 is connected by pipeline with mixer 11, and sample inlet selector valve C7 passes through
Pipeline is connected with sample pump C9, and sample pump C9 is connected by pipeline with pressure sensor C8, pressure sensor C8 and mixing
Device 11 is respectively connected to sampling valve 12 by pipeline, and sampling valve 12 is connect by pipeline with column position switching valve A13, column position switching valve
14 entrance of A13 connections chromatographic column, column position switching valve B15 connections chromatographic column 14 export, and column position switching valve B15 passes through piping connection pH
Detector 16, for pH detectors 16 by piping connection conductivity detector 17, conductivity detector 17 is ultraviolet by piping connection
Detector 18, UV detector 18 pass through piping connection Fraction collection valve 19.
Entrance selector valve A and entrance selector valve B is multi-center selection valve, and the optional mouth of valve is 1~10, passes through valve port
Automatic switchover different buffer solution may be selected.
Sample selector valve is multi-center selection valve, and the optional mouth of valve is 1~10, can automatically switch to select different
Sample avoids cross contamination.
A large amount of sample loading may be implemented in sample pump C, and a small amount of or micro-example loading may be implemented in sampling valve.
PH detectors, conductivity detector and UV detector can be with on-line real-time measuremen pH value, conductivity values and ultraviolet
Absorption value.
The beneficial effects of the utility model are：Using integrated integrated design, profit Protein Separation of computerizeing control is pure
Change process, can be automatically performed the process that isolates and purifies of protein, and high degree of automation can save a large amount of human resources；This
Invention can on-line detecting system the kinds of processes parameter such as pH value, conductivity value and ultraviolet absorption value, ensure product quality and
Purity.
Description of the drawings：
Fig. 1 is the structural schematic diagram of the utility model
Specific code name is as follows in figure：
1, entrance selector valve A
2, pressure sensor A
3, infusion pump A
4, entrance selector valve B
5, pressure sensor B
6, infusion pump B
7, sample inlet selector valve C
8, pressure sensor C
9, sampling pump C
10, three-way connection
11, mixer
12, sampling valve
13, column position switching valve A
14, chromatographic column
15, column position switching valve B
16, pH detectors
17, conductivity detector
18, UV detector
19, Fraction collection valve
20, cabinet.
Specific implementation mode：
The utility model can be automatically performed Protein Separation process, introduce this practicality by taking loading and elution process as an example below
The novel course of work.
The loading process of the utility model is：Sample by sample inlet selector valve C enter sample pump C, sample pump C according to
For the flow velocity of computer control system setting by sample delivery to pressure sensor C, pressure sensor C transmits loading pressure signal
To computer control system, sample enters sampling valve through pressure sensor C, enters column position switching valve A through sampling valve, passes through column position
The switch sample of switching valve A enters presetting target chromatographic column, completes the loading process of entire sample.
The elution process of the utility model is：Buffer solution A solvent enters infusion pump A, infusion pump A by entrance selector valve A
Buffer solution A is delivered to pressure sensor A according to the flow velocity of computer control system setting, pressure sensor A is by pressure signal
It is transmitted to computer control system, buffer solution A enters mixer through pressure sensor A by three-way connection, meanwhile, buffer solution B
Solvent enters infusion pump B by entrance selector valve B, and the flow velocity that infusion pump B is set according to computer control system is defeated by buffer solution B
It send to pressure sensor B, pressure sensor B and pressure signal is transmitted to computer control system, buffer solution B is through pressure sensor
B enters mixer by three-way connection, and buffer solution A and buffer solution B bypass into after being sufficiently mixed in a mixer through sampling valve
Column position switching valve A switches into target chromatographic column by column position switching valve A, and buffer solution washes the sample in chromatographic column
De-, the sample after elution is flowed out through column position switching valve B, passes sequentially through pH detectors, conductivity detector and UV detector, pH
Detector, conductivity detector and UV detector are by the pH signals detected, conductivity signal and UV absorption signal transmission
To computer control system, meanwhile, computer control system controls Fraction collection valve according to ultraviolet absorption value and conductivity value
Specified valve port open, setting valve port outflow of the liquid through Fraction collection valve, entire elution process is completed.
Above example is merely illustrative of the invention's technical idea, and protection scope of the present invention cannot be limited with this, all
It is any change done on the basis of technical solution according to technological thought proposed by the present invention, each falls within present invention protection model
Within enclosing.
Claims (3)
1. a kind of laboratory separation and purification of protein system, including entrance selector valve A（1）, pressure sensor A（2）, infusion pump A
（3）, entrance selector valve B（4）, pressure sensor B（5）, infusion pump B（6）, sample inlet selector valve C（7）, pressure sensor C
（8）, sample pump C（9）, three-way connection（10）, mixer（11）, sampling valve（12）, column position switching valve A（13）, chromatographic column（14）、
Column position switching valve B（15）, pH detectors（16）, conductivity detector（17）, UV detector（18）, Fraction collection valve（19）、
System chassis（20）；Wherein, entrance selector valve A（1）Pass through pipeline and infusion pump A（3）It is connected, infusion pump A（3）Pass through pipeline
With pressure sensor A（2）It is connected, entrance selector valve B（4）Pass through pipeline and infusion pump B（6）It is connected, infusion pump B（6）It is logical
Cross pipeline and pressure sensor B（5）It is connected, pressure sensor A（2）With pressure sensor B（5）It is connected by pipeline access three
Head（10）, three-way connection（10）Pass through pipeline and mixer（11）It is connected, sample inlet selector valve C（7）Pass through pipeline and sample
Product pump C（9）It is connected, sample pumps C（9）Pass through pipeline and pressure sensor C（8）It is connected, pressure sensor C（8）And mixing
Device（11）It is respectively connected to sampling valve by pipeline（12）, sampling valve（12）Pass through pipeline and column position switching valve A（13）Connection, column position
Switching valve A（13）Connect chromatographic column（14）Entrance, column position switching valve B（15）Connect chromatographic column（14）Outlet, column position switching valve B
（15）Pass through piping connection pH detectors（16）, pH detectors（16）Pass through piping connection conductivity detector（17）, conductivity
Detector（17）Pass through piping connection UV detector（18）, UV detector（18）Pass through piping connection Fraction collection valve
（19）.
2. a kind of laboratory separation and purification of protein system according to claim 1, it is characterised in that：Entrance selector valve A（1）
With entrance selector valve B（4）It is multi-center selection valve, the optional mouth of valve is 1~10.
3. a kind of laboratory separation and purification of protein system according to claim 1, it is characterised in that：Sample inlet selector valve
C（7）It is multi-center selection valve, the optional mouth of valve is 1~10.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721812349.0U CN207816912U (en) | 2017-12-22 | 2017-12-22 | A kind of laboratory separation and purification of protein system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721812349.0U CN207816912U (en) | 2017-12-22 | 2017-12-22 | A kind of laboratory separation and purification of protein system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207816912U true CN207816912U (en) | 2018-09-04 |
Family
ID=63330391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721812349.0U Active CN207816912U (en) | 2017-12-22 | 2017-12-22 | A kind of laboratory separation and purification of protein system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207816912U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110790813A (en) * | 2019-11-19 | 2020-02-14 | 上海多宁生物科技有限公司 | Multi-channel protein purification instrument |
-
2017
- 2017-12-22 CN CN201721812349.0U patent/CN207816912U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110790813A (en) * | 2019-11-19 | 2020-02-14 | 上海多宁生物科技有限公司 | Multi-channel protein purification instrument |
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| GR01 | Patent grant | ||
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| CP01 | Change in the name or title of a patent holder |
Address after: 223005 No. 1-9, Jixian Road, Huai'an Economic Development Zone, Jiangsu Province Patentee after: Jiangsu Hanbang Technology Co.,Ltd. Address before: 223005 No. 1-9, Jixian Road, Huai'an Economic Development Zone, Jiangsu Province Patentee before: JIANGSU HANBON SCIENCE & TECHNOLOGY Co.,Ltd. |