CN208636264U - A kind of on-line automatic pretreating device of aflatoxin based on flow path switching - Google Patents
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching Download PDFInfo
- Publication number
- CN208636264U CN208636264U CN201821241848.3U CN201821241848U CN208636264U CN 208636264 U CN208636264 U CN 208636264U CN 201821241848 U CN201821241848 U CN 201821241848U CN 208636264 U CN208636264 U CN 208636264U
- Authority
- CN
- China
- Prior art keywords
- way valves
- sample
- flow path
- path switching
- line automatic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching, including the changeable flow path being made of two multiple-way valves, I, two multiple-way valve II, syringe pump and multiple sample solutions, cleaning solution, pretreatment column is connected in two multiple-way valves I, quantitative loop is connected in two multiple-way valves II, two multiple-way valves I are connected between syringe pump and two multiple-way valves II；When changeable flow path cleaning finishes sample introduction, switch the access of two multiple-way valves, I, two multiple-way valve II, sample solution successively passes through syringe pump, two multiple-way valves I and pretreatment column, two multiple-way valves II and quantitative loop and completes sample introduction；Two II switched paths of multiple-way valve after completion sample introduction utilize flowing phase separation to carry out sample sample presentation.The utilization rate of processing sample can be improved in the utility model；The dosage for reducing reagent reduces the cost of analysis.
Description
Technical field
The utility model belongs to technical field of chromatographic analysis, and in particular to it is a kind of based on flow path switching aflatoxin exist
The automatic pretreating device of line.
Background technique
In chromatographic analysis system, if having interfering substance in delivery sample or testing sample solution or being not intended to
Impurity composition when, the detection characteristic and precision of sample can be seriously affected, in some instances it may even be possible to cause sample to be tested detection failure.
Aflatoxin (AFT) is the similar compound of a kind of chemical structure, is the derivative of dihydrofuran cumarin.It is yellow
Aspertoxin is mainly the secondary metabolite generated by aspergillus flavus aspergillus parasiticus, is occurred in damp-heat area food and feed yellow
The probability highest of aspertoxin.They are present in soil, animals and plants, in various nuts, be especially easy pollution peanut, corn,
The grain oil products such as rice, soybean, wheat are that mycotoxicosis is maximum, mould to human health risk one kind extremely outstanding
Verticillium toxin.Therefore, its detection efficiently and quickly is identified, to guarantee people's life health and safe important in inhibiting.
Detection, the analysis of aflatoxin, traditional processing mode is all first to handle sample introduction again, the processing utilization rate of sample
Only 1% or so, utilization rate is lower, reagent dosage is big, analysis cost is high.
Disclosed in Chinese patent (Authorization Notice No. CN202522549U) " a kind of Full-automatic aflatoxin analytic system ",
Include: a Full-automatic solid phase extraction system, is used to carry out Solid Phase Extraction；One two six-way valves, in be equipped with a trapping column, two
There are six mouth, trapping column is connected between third mouth and the 6th mouthful position six-way valve tool, the 5th mouthful of connection full-automatic solid phase
The output of extracting system, the 4th mouthful of one waste liquid bottle of connection；One highly effective liquid phase chromatographic system a comprising liquid-phase chromatographic column, a column
Deriving device, one first infusion pump and a fluorescence detector, liquid-phase chromatographic column are connected with the first of two six-way valves afterwards, and first
Second mouthful of infusion pump and two six-way valves is connected, the post-column derivation device and the liquid-phase chromatographic column phase
Even, for carrying out post-column derivation to chromatography column effluent, the fluorescence detector is connected with the post-column derivation device, uses
In progress fluorescence detection.
The analysis system uses switching valve and trapping column, and a Full-automatic solid phase extraction system and one are equipped with automatic column
The highly effective liquid phase chromatographic system of deriving device and fluorescence detector carries out online afterwards, by the automation for controlling software realization system
Analysis operation.Wherein, full-automatic extraction equipment is equivalent to the pre-processing device of aflatoxins toxin sample solution, but the processing side
Formula is complex, and limited to the cleaning of extraction equipment, therefore, still remains the lower problem of sample utilisation.
Utility model content
The utility model is a kind of on-line automatic pretreating device of aflatoxin based on flow path switching, for aspergillus flavus
The particularity of toxin pre-treatment realizes a kind of new on-line automatic pretreating device based on flow path switching, utilizes aflatoxin
With reservation difference of the matrix on pretreatment column, the matrix of aflatoxin in complicated substrate is eliminated, realizes various samples
Online pretreatment.
In order to realize the purpose of this utility model, using following technical scheme:
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching, including by two multiple-way valve I, two
The changeable flow path that multiple-way valve II, syringe pump and multiple sample solutions, cleaning solution are constituted, the sample solution and cleaning solution switching
It is completed by matched device with sample introduction function, syringe pump carries out imbibition and sample introduction switching by triple valve, described more than two
It is connected with pretreatment column in port valve I, quantitative loop is connected in two multiple-way valves II, two multiple-way valves I are connected on syringe pump and two
Between the multiple-way valve II of position；The sample solution, the outlet of cleaning solution and syringe pump inlet communication, the outlet two of syringe pump
Position I entrance of multiple-way valve；When changeable flow path cleaning finishes sample introduction, switch the access of two multiple-way valves, I, two multiple-way valve II, sample
Product solution successively passes through syringe pump, two multiple-way valves I and pretreatment column, two multiple-way valves II and quantitative loop and completes sample introduction；
Two II switched paths of multiple-way valve after completion sample introduction utilize flowing phase separation to carry out sample sample presentation.
In order to further realize the purpose of this utility model, following technical scheme can also be used:
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, the pretreatment column
For Multifunctional cleanup column or immune affinity column.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, the pretreatment column
In be provided with the filler of filtration.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, two multi-pass
I, two multiple-way valve II of valve is two ten-way valves or two six-way valves.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, two multi-pass
I, two II access of multiple-way valve of valve is switched to Electronic control.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, two multi-pass
I, two multiple-way valve II of valve, which corresponds on the position mouthful that cleaning solution cleaning is discharged, connects collection vessel.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, the collection vessel
For waste liquid bottle.
A kind of on-line automatic pretreating device of aflatoxin based on flow path switching as described above, two multi-pass
Valve I, which is connected with, carries out reversed regenerated peristaltic pump for pretreatment column.
Compared with prior art, the utility model has the beneficial effects that
1, the utilization rate of processing sample can be improved in the utility model, this pretreating device only handles the ul rank of analysis
Sample, sample utilisation is high；The dosage for reducing reagent reduces the cost of analysis.
2, pretreatment column can need to select Multifunctional cleanup column or immune affinity column according to sample.Pretreatment column is repeatable
It uses, and renewable；And the use of consumptive material in analytic process is reduced, it further reduces the cost.
3, two multiple-way valves of the utility model, I, two multiple-way valve II can use two ten-way valves, two six-way valves
Or multiple valve groups of similar effect are closed to realize the function, with a variety of pretreatment technologies of compatibility and a variety of processing modes；Meanwhile it can
According to the difference of sample matrices, flexible combination collocation is used, to guarantee the efficiency and sample utilisation of processing.
Detailed description of the invention
Fig. 1 is the system composition schematic diagram of the utility model.
Appended drawing reference: 1- sample solution 1,2- sample solution 2,3- sample solution 3,4- cleaning solution, 5- syringe pump, 6- threeway
Valve, bis- multiple-way valves I of 7-, 71- pretreatment column, bis- multiple-way valves II of 8-, 81- quantitative loop, 9- chromatographic column, 10- detector, 11-
Sample introduction pipeline, 12- peristaltic pump, 13- waste liquid bottle.
Specific embodiment
To keep the purpose of this utility model, technical solution and advantage clearer, below in conjunction with the attached of the utility model
Figure, is clearly and completely described the technical solution of the utility model, it is clear that described embodiment is the utility model
A part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art exist
Every other embodiment obtained under the premise of creative work is not made, is fallen within the protection scope of the utility model.
As shown in Figure 1, a kind of on-line automatic pre-treatment dress of aflatoxin based on flow path switching disclosed in the present embodiment
It sets, it is changeable including what is be made of two multiple-way valves, I 7, two multiple-way valves II 8, syringe pump 5 and multiple sample solutions, cleaning solution 4
Flow path.
Sample solution, cleaning solution 4 are arranged in the import of syringe pump 5, sample solution and cleaning solution switching by it is mating have into
The device of sample function is completed, and syringe pump carries out imbibition by triple valve and sample introduction switches, and passes through existing switching device, such as three
Port valve 6 and control unit cooperation, realize cleaning and respectively sample introduction.It is connected with pretreatment column 71 in two multiple-way valves I 7, two
Quantitative loop 81 is connected in multiple-way valve II 8.
Two multiple-way valves I 7 are connected between syringe pump 5 and two multiple-way valves II 8, sample solution, cleaning solution 4 outlet with
The inlet communication of syringe pump 5, two I 7 entrances of multiple-way valve of outlet of syringe pump 5, syringe pump 5 is by triple valve 6 to sample
Solution and cleaning solution 4 realize feed liquor switching.
When changeable flow path cleaning finishes sample introduction, switch the access of two multiple-way valves, I 7, two multiple-way valves II 8, sample is molten
Liquid successively passes through 71, two multiple-way valves II 8 of 5, two multiple-way valves I 7 of syringe pump and pretreatment column and quantitative loop 81 complete into
Sample.
Two II 8 switched paths of multiple-way valve after completion sample introduction send sample by sample introduction pipeline 11 using flowing phase separation
Sample detection, analysis, complete the automatic pre-treatment of sample.
Two multiple-way valves, I 7, two multiple-way valves II 8 are two ten-way valves or two six-way valves, and clean and arrange in cleaning solution 4
Collection vessel is connected on position mouthful out；Collection vessel uses waste liquid bottle, convenient for being collected to cleaning solution 4, emissions operation.
The state of two sets of two six-way valves in following embodiment is location A, dotted line with the display connection of solid line shown in Fig. 1
Display connection is B location.Two six-way valves are switched over by Electronic control in A, B location, to change sample solution, cleaning
The flow path of liquid 4.
Embodiment 1:
Sample relatively simple for matrixes such as peanut, wheat, corns, pretreatment column 71 uses Multifunctional cleanup column, right
Sample solution carries out purification post analysis.
As shown in Figure 1, sample solution has three tunnels, respectively sample solution 1, sample solution 2, sample solution 3, cleaning solution 4
For pure water, setting is arranged in 6 inlet side of triple valve, two multiple-way valves I 7 in 6 outlet side of triple valve, utilizes syringe pump 5 or multi-pass
Valve, autosampler complete the switching of three road sample solutions, cleaning solution 4.Chromatographic column 9 uses model C18 series filler, pre- to locate
It manages column 71 and uses SHAC-2 series filler.
In use, two multiple-way valves II 8 are located at position shown in Fig. 1 firstly, two multiple-way valves I 7 are shifted to B location
It is constant, pretreatment column 71, quantitative loop 81 etc. are cleaned using pure water；After cleaning, two multiple-way valves II 8 switch to B
Position, syringe pump 5 draw suitable sample solution, two multiple-way valves I 7 are then injected into, by the pretreatment of Multifunctional cleanup column
After column 71, into the quantitative loop 81 of two multiple-way valves II 8；After completing sample introduction, two multiple-way valves II 8 switch to location A, so
Sample is separated in the case where flowing phase separation by chromatographic column 9 afterwards, is analyzed into fluorescence detector 10.
Embodiment 2:
The sample relative complex for matrix, pretreatment column 71 use immune affinity column or have the pre-treatment of similar function
Column carries out purification post analysis to sample solution.
As shown in Figure 1, before syringe pump 5 and two multiple-way valves, I 7, two multiple-way valves II 8 are identical as embodiment 1.
In use, two multiple-way valves II 8 are located at shown in Fig. 1 A firstly, two multiple-way valves I 7 are switched to B location
It sets, syringe pump 5 draws appropriate amount of sample, the aflatoxin for being then injected into two I 7 cocurrents of multiple-way valve through pre-treatment column, in sample
It is pretreated the absorption of column 71；Syringe pump 5 sucks suitable elution buffer, is then injected into two multiple-way valves I 7 and pre-treatment column
It is sufficiently displaced from original sample.Static 1min elutes elution buffer sufficiently the aflatoxin that pre-treatment is adsorbed,
Two multiple-way valves II 8 switch to B location, and syringe pump 5 continues buffer elution liquid being pushed into two multiple-way valves II 8 and its quantitative loop
In.After completing sample introduction, two multiple-way valves II 8 switch to location A, carry out analysis detection.
Two multiple-way valves I 7 switch to location A, and peristaltic pump 12 provides regenerated liquid, reversed flushing is carried out to pretreatment column 71
Regeneration；Regenerated liquid after flushing, in the correspondence position of location A mouthful, is collected into waste liquid bottle 13 by two multiple-way valves I 7.
The technology contents of the not detailed description of the utility model are well-known technique.
Claims (8)
1. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching, including by two multiple-way valves more than I, two
Port valve II, syringe pump and multiple sample solutions, cleaning solution constitute changeable flow path, the sample solution and cleaning solution switching by
The matched device with sample introduction function is completed, and syringe pump carries out imbibition by triple valve and sample introduction switches, which is characterized in that institute
It states and is connected with pretreatment column in two multiple-way valves I, quantitative loop is connected in two multiple-way valves II, two multiple-way valves I are connected on note
It penetrates between pump and two multiple-way valves II；The sample solution, the outlet of cleaning solution and syringe pump inlet communication, syringe pump go out
Mouth two I entrances of multiple-way valve of connection；When changeable flow path cleaning finishes sample introduction, switch two multiple-way valves, I, two multiple-way valve II
Access, it is complete that sample solution successively passes through syringe pump, two multiple-way valves I and pretreatment column, two multiple-way valves II and quantitative loop
At sample introduction；Two II switched paths of multiple-way valve after completion sample introduction utilize flowing phase separation to carry out sample sample presentation.
2. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1, special
Sign is that the pretreatment column is Multifunctional cleanup column or immune affinity column.
3. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1 or 2,
It is characterized in that, the filler of filtration is provided in the pretreatment column.
4. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1, special
Sign is that two multiple-way valves, I, two multiple-way valve II is two ten-way valves or two six-way valves.
5. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1, special
Sign is that two multiple-way valves, I, two II access of multiple-way valve is switched to Electronic control.
6. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1, special
Sign is that two multiple-way valves, I, two multiple-way valve II, which corresponds on the position mouthful that cleaning solution cleaning is discharged, connects collection vessel.
7. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 6, special
Sign is that the collection vessel is waste liquid bottle.
8. a kind of on-line automatic pretreating device of aflatoxin based on flow path switching according to claim 1, special
Sign is that two multiple-way valves I, which are connected with, carries out reversed regenerated peristaltic pump for pretreatment column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821241848.3U CN208636264U (en) | 2018-08-02 | 2018-08-02 | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821241848.3U CN208636264U (en) | 2018-08-02 | 2018-08-02 | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208636264U true CN208636264U (en) | 2019-03-22 |
Family
ID=65739629
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821241848.3U Active CN208636264U (en) | 2018-08-02 | 2018-08-02 | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208636264U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108845056A (en) * | 2018-08-02 | 2018-11-20 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching |
| CN110095549A (en) * | 2019-06-12 | 2019-08-06 | 吉林省产品质量监督检验院(吉林省农产品认证中心) | The detection device and method of aflatoxin in a kind of Grain and its product |
-
2018
- 2018-08-02 CN CN201821241848.3U patent/CN208636264U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108845056A (en) * | 2018-08-02 | 2018-11-20 | 山东省农业科学院农业质量标准与检测技术研究所 | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching |
| CN110095549A (en) * | 2019-06-12 | 2019-08-06 | 吉林省产品质量监督检验院(吉林省农产品认证中心) | The detection device and method of aflatoxin in a kind of Grain and its product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108845056A (en) | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching | |
| CN102166433B (en) | Multichannel parallel automatic solid phase extraction device | |
| CN102262060A (en) | Method and device for analyzing heavy metal elements by on-line enrichment and atomic absorption spectrum | |
| CN103235074B (en) | High performance liquid chromatography online analysis method and application thereof | |
| CN208636264U (en) | A kind of on-line automatic pretreating device of aflatoxin based on flow path switching | |
| CN105606753A (en) | FAE (field assisted extraction)-separation and enrichment-chromatographic analysis on-line combination equipment | |
| CN104034817A (en) | Ion chromatography on-line pretreatment for measuring glyphosate in genetically modified soybean oil | |
| CN102062711A (en) | Organic pollutant enriching device and method for pretreatment of water sample with bulk mass | |
| CN102967495B (en) | Sample pretreatment device and sample treatment method | |
| CN107589190A (en) | The double SPE high performance liquid chromatography on-line coupling equipment of large volume sample injection | |
| CN109959741B (en) | Dual-channel online analysis pretreatment and offline analysis integrated liquid chromatograph and application method thereof | |
| CN109959742B (en) | Online analysis pretreatment and offline analysis integrated liquid chromatograph and use method thereof | |
| CN209858493U (en) | Dual-channel online analysis pretreatment and offline analysis integrated liquid chromatograph | |
| CN211877889U (en) | Chromatograph-mass spectrometer | |
| CN202942682U (en) | Solid-phase extraction column | |
| CN207457159U (en) | More flux liquid-phase chromatographic column circle irrigation systems | |
| CN209858497U (en) | Pretreatment and analysis integrated device for online detection of benzo (a) pyrene in hot-pressed vegetable oil | |
| CN209858494U (en) | Online analysis pretreatment and offline analysis integrated liquid chromatograph | |
| CN211111880U (en) | Organic matter extraction-separation-cleaning-recovery device | |
| CN202886166U (en) | Sample pretreatment device | |
| CN110286173A (en) | Liquid-chromatography apparatus is used in a kind of detection of blood concentration | |
| CN110286175A (en) | A kind of multidimensional on-line solid phase extraction liquid-chromatography apparatus | |
| CN208071618U (en) | A kind of protein separation detection unit | |
| CN110455942A (en) | A kind of online Ultra Performance Liquid Chromatography instrument of three-dimensional | |
| CN205643264U (en) | Gaseous transmission and detection device of gas chromatography sample |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20230926 Address after: 250100 No. 23788, Gongye North Road, Licheng District, Jinan City, Shandong Province Patentee after: SHANDONG ACADEMY OF AGRICULTURAL SCIENCES Patentee after: QINGDAO SHENGHAN CHROMATOGRAPHY TECHNOLOGY Co.,Ltd. Address before: 250100 No. 202 industrial North Road, Shandong, Ji'nan Patentee before: INSTITUTE OF QUALITY STANDARDS & TESTING TECHNOLOGY FOR AGRO-PRODUCTS OF SHANDONG ACADEMY OF AGRICULTURAL SCIENCES Patentee before: QINGDAO XINGUANG ZHIYUAN ENVIRONMENT TECHNOLOGY Co.,Ltd. |