CN111965277A - Automatic collection device for antibody purification effluent liquid - Google Patents

Automatic collection device for antibody purification effluent liquid Download PDF

Info

Publication number
CN111965277A
CN111965277A CN202010782108.6A CN202010782108A CN111965277A CN 111965277 A CN111965277 A CN 111965277A CN 202010782108 A CN202010782108 A CN 202010782108A CN 111965277 A CN111965277 A CN 111965277A
Authority
CN
China
Prior art keywords
antibody
chamber
connecting pipe
agglutination
effluent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010782108.6A
Other languages
Chinese (zh)
Inventor
杨思源
毕广影
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tianjin Kedetong Biotechnology Co ltd
Original Assignee
Tianjin Kedetong Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tianjin Kedetong Biotechnology Co ltd filed Critical Tianjin Kedetong Biotechnology Co ltd
Priority to CN202010782108.6A priority Critical patent/CN111965277A/en
Publication of CN111965277A publication Critical patent/CN111965277A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Abstract

The invention provides an automatic collection device for antibody purification effluent, which comprises a conveying module, a detection module and an analysis module, wherein the detection module comprises an agglutination part and an identification part, the agglutination part comprises an agglutination chamber communicated with the conveying module, a quantification cylinder communicated with the agglutination chamber through a connecting pipe I, a first electromagnetic valve arranged on the connecting pipe I, a quantification mechanism connected with the quantification cylinder, a liquid storage barrel connected with the quantification cylinder through a connecting pipe II, and a second electromagnetic valve arranged on the connecting pipe II. The automatic collection device for the antibody purification effluent prepared by the technical scheme of the invention combines the target antibody with the latex particles in the agglutination chamber, improves the probability of combination of the antibody and the specific binding protein, can identify the target antibody when the content of the target antibody in the effluent is low, can color the effluent after identification, and detects the concentration of the target antibody in the effluent, thereby integrally improving the collection precision of the effluent and reducing the waste.

Description

Automatic collection device for antibody purification effluent liquid
Technical Field
The invention belongs to the technical field of biological pharmacy, and particularly relates to an automatic collection device for antibody purification effluent liquid.
Background
The production process of the antibody medicine comprises the steps of fermentation, purification, preparation and the like. The liquid chromatography is the core step of the antibody drug purification process, and the liquid chromatography process generally comprises the steps of balancing, loading, washing, eluting, regenerating and the like, and relates to the collection of elution effluent containing target protein and the discharge of effluent in other steps.
In the publication of: CN207727013U discloses an automatic collection device for antibody-purified effluent, which can identify target proteins in the effluent and determine whether the target proteins have activity, but the device has the following disadvantages:
1. the contact time of the antibody and the specific binding protein is short, and particularly when the content of the target antibody in effluent is low, the target antibody is difficult to detect;
2. the content of the target antibody in the effluent cannot be detected.
Disclosure of Invention
The invention aims to solve the problems and designs an automatic collection device for antibody purification effluent.
The technical scheme of the invention is that the automatic collection device for the antibody purification effluent comprises a conveying module, a detection module and an analysis module, wherein the detection module comprises an agglutination part and an identification part, the agglutination part comprises an agglutination chamber communicated with the conveying module, a quantitative cylinder communicated with the agglutination chamber through a connecting pipe, a first electromagnetic valve arranged on the connecting pipe, a quantitative mechanism connected with the quantitative cylinder, a liquid storage barrel connected with the quantitative cylinder through a connecting pipe II, and a second electromagnetic valve arranged on the connecting pipe II.
The quantitative mechanism comprises a servo electric cylinder and a quantitative rod connected to the output end of the servo electric cylinder, and the quantitative rod is slidably embedded in the quantitative cylinder.
The quantitative cylinder is characterized in that an induction iron block is arranged on the quantitative rod, an electromagnet is installed on the inner wall of the quantitative cylinder, and the induction iron block is electrically connected with the electromagnet.
The identification part comprises a third connecting pipe communicated with the agglutination chamber at one end, an identification chamber connected with the other end of the third connecting pipe, a third electromagnetic valve arranged on the third connecting pipe and a surface plasma resonance sensor arranged on the identification chamber.
The conveying module comprises a main pipeline, a first branch pipe and a second branch pipe which are connected to one end of the main pipeline, a first shunt pipe arranged on the main pipeline and a second shunt pipe arranged on the first branch pipe.
The first shunt pipe is communicated with the agglutination chamber.
The junction of trunk line, first branch pipe and second branch pipe is provided with the electromagnetism three-way valve, the export of first branch pipe and second branch pipe is gone out and is all provided with the container.
The analysis module comprises a coloring chamber and a spectrophotometer connected with the coloring chamber through a connecting pipe.
The coloring chamber is communicated with the second shunt pipe, and the fourth connecting pipe is provided with a fourth electromagnetic valve.
The plasma resonance control system is characterized by further comprising an electronic controller and a computer electrically connected with the electronic controller, wherein the electronic controller is electrically connected with the first electromagnetic valve, the servo electric cylinder, the electromagnet, the second electromagnetic valve, the plasma resonance sensor, the three electromagnetic valve, the third electromagnetic valve and the fourth electromagnetic valve respectively.
The automatic collection device for the antibody purification effluent liquid, which is manufactured by the technical scheme of the invention, has the beneficial effects that: the latex solution is added into the agglutination chamber, so that the target antibody is combined with latex particles, the probability of combining the antibody with the specific binding protein is improved, the target antibody can be identified when the content of the target antibody in the effluent is low, meanwhile, the effluent can be colored in the coloring chamber after identification, the concentration of the target antibody in the effluent is detected through a spectrophotometer, the accuracy of effluent collection is integrally improved, and waste is reduced.
Drawings
FIG. 1 is a schematic diagram of an overall system;
FIG. 2 is a schematic view of a detection module;
FIG. 3 is a schematic diagram of an analysis module;
FIG. 4 is a schematic view of a dosing mechanism;
fig. 5 is a schematic diagram of electrical connection of the components.
In the figure: 1. a delivery module; 2. a detection module; 3. an analysis module; 4. an agglutination chamber; 5. a first connecting pipe; 6. a dosing cylinder; 7. a first electromagnetic valve; 8. a second connecting pipe; 9. a liquid storage barrel; 10. a second electromagnetic valve; 11. a servo electric cylinder; 12. a dosing rod; 13. induction of iron blocks; 14. an electromagnet; 15. a third connecting pipe; 16. an identification chamber; 17. a third electromagnetic valve; 18. a surface plasmon resonance sensor; 19. a main pipeline; 20. a first branch pipe; 21. a second branch pipe; 22. a first shunt pipe; 23. a second shunt pipe; 24. an electromagnetic three-way valve; 25. a container; 26. a coloring chamber; 27. a fourth connecting pipe; 28. a spectrophotometer; 29. a fourth electromagnetic valve; 30. an electronic controller; 34. and (4) a computer.
Detailed Description
Referring to the drawings, the detailed description of the specific embodiments of the present invention will be made, and as shown in fig. 1, 2 and 4, an automatic collection device for antibody purification effluent comprises a conveying module 1, a detection module 2 and an analysis module 3, wherein the detection module 2 comprises an agglutination part and a recognition part, the agglutination part comprises an agglutination chamber 4 communicated with the conveying module 1, a quantification cylinder 6 communicated with the agglutination chamber 4 through a first connecting pipe 5, a first electromagnetic valve 7 installed on the first connecting pipe 5, a quantification mechanism connected with the quantification cylinder 6, a liquid storage barrel 9 connected with the quantification cylinder 6 through a second connecting pipe 8, and a second electromagnetic valve 10 installed on the second connecting pipe 8, the quantification mechanism comprises a servo electric cylinder 11 and a quantification rod 12 connected to the output end of the servo electric cylinder 11, the quantification rod 12 is slidably embedded in the quantification cylinder 6, and an induction iron block 13 is arranged on the quantification rod 12, install electro-magnet 14 on the inner wall of quantitative cylinder 6, response iron plate 13 and 14 electric connection of electro-magnet, the transport module includes trunk line 19, connects first branch pipe 20 and the second branch pipe 21 of locating 19 one ends of trunk line, sets up first branch pipe 22 on trunk line 19 and sets up second branch pipe 23 on first branch pipe 20, first branch pipe 23 and agglutination chamber 4 put through mutually, and the junction of trunk line 19, first branch pipe 20 and second branch pipe 21 is provided with electromagnetism three-way valve 24, the export of first branch pipe 20 and second branch pipe 21 is gone out and is all is provided with container 25.
In this embodiment, the delivery module 1 is used for delivering the antibody-purified effluent, the detection module 2 is used for detecting the antibody-purified effluent to determine whether the antibody-purified effluent contains the target antibody and whether the target antibody has binding activity, and the analysis module 3 is used for detecting the content of the target antibody in the antibody-purified effluent.
The effluent liquid of antibody purification flows in from the main pipeline 19, when flowing through the main pipeline 19, part of the effluent liquid flows into the agglutination chamber 4 through the first shunt pipe 22, meanwhile, the latex solution in the liquid storage barrel 9 enters the quantification cylinder 6 through the second connecting pipe 8 under the action of the quantification mechanism, then enters the agglutination chamber 4 through the first connecting pipe 5 under the action of the quantification mechanism, and is mixed with the effluent liquid in the agglutination chamber 4, after the mixing is completed for a period of time, the antibody in the effluent liquid can be wrapped by the latex particles, and the combination probability of the target antibody and the specific protein can be improved.
The quantitative mechanism can quantitatively convey the latex solution from the liquid storage barrel 9 to the agglutination chamber 4, and the whole conveying process is as follows: servo electric cylinder 11 starts, solenoid valve 7 is opened simultaneously, servo electric cylinder 11 output drives the ration pole 12 and slides in a quantification section of thick bamboo 6, extrude the latex solution in a quantification section of thick bamboo 6 to agglutinate the room 4 in through connecting pipe 5, mix with the effluent liquid in the agglutination room 4, when response iron plate 13 on the ration pole 12 moves the position of electro-magnet 14 in the quantification section of thick bamboo 6, servo electric cylinder 11 stop motion, at this moment, solenoid valve 7 is closed, solenoid valve two 10 is opened, servo electric cylinder 11 drives the ration pole 12 and moves back in a quantification section of thick bamboo 6, at this in-process, the latex solution in the liquid reserve tank 9 is under atmospheric pressure's effect, can enter into in a quantification section of thick bamboo 6 through connecting pipe two 8.
The identification part comprises a third connecting pipe 15 communicated with the agglutination chamber 4 at one end, an identification chamber 16 connected with the other end of the third connecting pipe 15, a third electromagnetic valve 17 arranged on the third connecting pipe 15 and a surface plasma resonance sensor 18 arranged on the identification chamber 16.
After the effluent liquid of antibody purification in the agglutination chamber 4 is mixed with the latex solution, the electromagnetic valve III 17 is opened, the mixed liquid enters the recognition chamber 16, the surface plasmon resonance sensor 18 is installed on the recognition chamber 16, meanwhile, the specific protein capable of being combined with the target antibody is attached to the surface of the surface plasmon resonance sensor 18, the binding rate of the target antibody and the specific protein is high under the wrapping of latex particles, even if the content of the antibody in the effluent liquid of antibody purification is low, the antibody can be recognized in the recognition chamber 16, the waste of the effluent liquid is avoided, and the collection precision is also improved.
As shown in fig. 3, the analysis module 3 includes a staining chamber 26 and a spectrophotometer 28 connected to the staining chamber 26 through a fourth connection pipe 27, the staining chamber 26 is connected to the second shunt pipe 23, and a fourth solenoid valve 29 is mounted on the fourth connection pipe 28.
In the detection module 2, after the agglutination treatment and specific recognition of the agglutination part and the recognition part, the effluent containing the target antibody flows out from the first branch pipe 20, during the outflow process, part of the effluent flows into the staining chamber 26 through the second branch pipe 23, the staining chamber is pre-filled with Coomassie brilliant blue G250 solution, the effluent can be combined with the antibody in the effluent for staining, after the staining is completed, the electromagnetic valve four 29 is opened, the stained liquid enters the spectrophotometer 28, and the content of the antibody in the liquid is detected.
As shown in fig. 5, the plasma processing system further comprises an electronic controller 30 and a computer 31 electrically connected to the electronic controller 30, wherein the electronic controller is electrically connected to the first solenoid valve 7, the servo electric cylinder 11, the electromagnet 14, the second solenoid valve 10, the plasma resonance sensor 18, the three-way solenoid valve 24, the third solenoid valve 17 and the fourth solenoid valve 29, respectively.
In this embodiment, the collecting device is further provided with an electronic controller and a computer, wherein the electronic controller 30 is electrically connected with the first solenoid valve 7, the servo cylinder 11, the electromagnet 14, the second solenoid valve 10, the plasmon resonance sensor 18, the three-way solenoid valve 24, the third solenoid valve 17 and the fourth solenoid valve 29 in the whole device for respectively controlling the coordination work among the components, and the computer 31 can perform more complicated and tedious operations and control through the computer due to the electronic controller 30.
In summary, the overall working process of the invention is as follows: first, the antibody purification effluent flows in from the main pipe 19, when flowing through the main pipe 19, part of the effluent will flow in from the first branch pipe 20 to the agglutination chamber 4, the quantitative mechanism quantitatively transfers the latex solution into the agglutination chamber 4 to mix with the effluent, then the mixed solution enters the identification chamber 16 through the third connecting pipe 15 to identify, if it is detected that the mixed solution does not contain the target antibody, the electronic controller 30 controls the electromagnetic three-way valve 24 to open to the second branch pipe 21, so that the effluent will flow out through the second branch pipe 21 to the container 25 at the outlet of the second branch pipe 21, if it is detected that the mixed solution contains the target antibody, the electronic controller 30 controls the electromagnetic three-way valve 24 to open to the first branch pipe 20, so that the effluent will flow in the first branch pipe 20, then the effluent in the first branch pipe 20 will flow down to the container 25 at the outlet of the first branch pipe 20, in the process, part of the effluent will flow into the staining chamber 26 through the second shunt 23 to be mixed with the coomassie brilliant blue G250 solution for staining, and then the electronic controller 30 controls the electromagnetic valve four 29 to be opened, and the stained effluent will enter the spectrophotometer 28 for detection, thereby helping the operator to analyze the content of the antibody in the effluent.
The automatic collection device for the antibody purification effluent liquid, which is manufactured by the technical scheme of the invention, has the beneficial effects that: the latex solution is added into the agglutination chamber, so that the target antibody is combined with latex particles, the probability of combining the antibody with the specific binding protein is improved, the target antibody can be identified when the content of the target antibody in the effluent is low, meanwhile, the effluent can be colored in the coloring chamber after identification, the concentration of the target antibody in the effluent is detected through a spectrophotometer, the accuracy of effluent collection is integrally improved, and waste is reduced.
While one embodiment of the present invention has been described in detail, the description is only a preferred embodiment of the present invention and should not be taken as limiting the scope of the invention. All equivalent changes and modifications made within the scope of the present invention shall fall within the scope of the present invention.

Claims (10)

1. An automatic collection device for antibody purification effluent liquid is characterized in that: including transport module (1), detection module (2) and analysis module (3), detection module includes agglutination portion and identification portion, the agglutination portion includes agglutination chamber (4) that communicates mutually with transport module (1), quantitative section of thick bamboo (6) that communicate mutually through connecting pipe (5) with agglutination chamber (4), install solenoid valve (7) on connecting pipe (5), the quantitative mechanism that meets with quantitative section of thick bamboo (6), stock solution bucket (9) that meet and install solenoid valve two (10) on connecting pipe two (8) through connecting pipe two (8) with quantitative section of thick bamboo (6).
2. The automatic antibody-purification effluent collection apparatus according to claim 1, wherein: the quantitative mechanism comprises a servo electric cylinder (11) and a quantitative rod (12) connected to the output end of the servo electric cylinder (11), and the quantitative rod (12) is slidably embedded in the quantitative cylinder (6).
3. The automatic antibody-purification effluent collection apparatus according to claim 2, wherein: be provided with response iron plate (13) on quantitative pole (12), install electro-magnet (14) on the inner wall of quantitative section of thick bamboo (6), response iron plate (13) and electro-magnet (14) electric connection.
4. The automatic antibody-purification effluent collection apparatus according to claim 1, wherein: the identification part comprises a third connecting pipe (15) communicated with the agglutination chamber (4) at one end, an identification chamber (16) connected with the other end of the third connecting pipe (15), a third electromagnetic valve (17) arranged on the third connecting pipe (15) and a surface plasma resonance sensor (18) arranged on the identification chamber (16).
5. The automatic antibody-purification effluent collection apparatus according to claim 1, wherein: the conveying module comprises a main pipeline (19), a first branch pipe (20) and a second branch pipe (21) which are connected to one end of the main pipeline (19), a first branch pipe (22) arranged on the main pipeline (19) and a second branch pipe (23) arranged on the first branch pipe (20).
6. The automatic antibody-purification effluent collection apparatus according to claim 5, wherein: the first shunt pipe (22) is communicated with the agglutination chamber (4).
7. The automatic antibody-purification effluent collection apparatus according to claim 5, wherein: the junction of main pipeline (19), first branch pipe (20) and second branch pipe (21) is provided with electromagnetism three-way valve (24), the export of first branch pipe (20) and second branch pipe (21) all is provided with container (25).
8. The automatic antibody-purification effluent collection apparatus according to claim 1, wherein: the analysis module comprises a staining chamber (26) and a spectrophotometer (28) connected with the staining chamber (26) through a connecting pipe four (27).
9. The automatic antibody-purification effluent collection apparatus according to claim 8, wherein: the coloring chamber (26) is communicated with the second shunt pipe (23), and the connecting pipe IV (27) is provided with a solenoid valve IV (29).
10. The automatic antibody-purification effluent collection apparatus according to claim 1, wherein: the plasma resonance control system is characterized by further comprising an electronic controller (30) and a computer (31) electrically connected with the electronic controller (30), wherein the electronic controller (30) is respectively and electrically connected with the first electromagnetic valve (7), the servo electric cylinder (11), the electromagnet (14), the second electromagnetic valve (10), the plasma resonance sensor (18), the three electromagnetic valve (24), the third electromagnetic valve (17) and the fourth electromagnetic valve (29).
CN202010782108.6A 2020-08-06 2020-08-06 Automatic collection device for antibody purification effluent liquid Pending CN111965277A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010782108.6A CN111965277A (en) 2020-08-06 2020-08-06 Automatic collection device for antibody purification effluent liquid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010782108.6A CN111965277A (en) 2020-08-06 2020-08-06 Automatic collection device for antibody purification effluent liquid

Publications (1)

Publication Number Publication Date
CN111965277A true CN111965277A (en) 2020-11-20

Family

ID=73364908

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010782108.6A Pending CN111965277A (en) 2020-08-06 2020-08-06 Automatic collection device for antibody purification effluent liquid

Country Status (1)

Country Link
CN (1) CN111965277A (en)

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2392387A1 (en) * 1976-12-10 1978-12-22 Technicon Instr METHOD FOR DEMONSTRATING AN ANTIGEN OR A PARTICULAR ANTIBODY IN A LIQUID
WO1991014944A1 (en) * 1990-03-28 1991-10-03 Tenta Properties N.V. A method for the collection of antibodies and the detection of a specific antibody species in a fluid sample and a device for carrying out said method as well as a kit comprising said device
US5215102A (en) * 1991-10-25 1993-06-01 La Mina Ltd. Capillary blood antigen testing apparatus
JP2006317401A (en) * 2005-05-16 2006-11-24 Matsushita Electric Ind Co Ltd Immunoreactive agglutinating agent for measuring immunoagglutination inhibiting reaction, measuring method using it and measuring device
CN203069431U (en) * 2012-12-20 2013-07-17 百奇生物科技（苏州）有限公司 Automatic protein detection purifying instrument
CN103698289A (en) * 2013-12-05 2014-04-02 成都雅途生物技术有限公司 Chromatographic column effluent detection device
CN207727013U (en) * 2017-12-21 2018-08-14 泰州迈博太科药业有限公司 A kind of antibody purification efflux automatic collecting device
CN210906083U (en) * 2019-11-02 2020-07-03 巴彦淖尔市金盛汇化工有限公司 Quantitative feeding device of methanesulfonic acid

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2392387A1 (en) * 1976-12-10 1978-12-22 Technicon Instr METHOD FOR DEMONSTRATING AN ANTIGEN OR A PARTICULAR ANTIBODY IN A LIQUID
WO1991014944A1 (en) * 1990-03-28 1991-10-03 Tenta Properties N.V. A method for the collection of antibodies and the detection of a specific antibody species in a fluid sample and a device for carrying out said method as well as a kit comprising said device
US5215102A (en) * 1991-10-25 1993-06-01 La Mina Ltd. Capillary blood antigen testing apparatus
JP2006317401A (en) * 2005-05-16 2006-11-24 Matsushita Electric Ind Co Ltd Immunoreactive agglutinating agent for measuring immunoagglutination inhibiting reaction, measuring method using it and measuring device
CN203069431U (en) * 2012-12-20 2013-07-17 百奇生物科技（苏州）有限公司 Automatic protein detection purifying instrument
CN103698289A (en) * 2013-12-05 2014-04-02 成都雅途生物技术有限公司 Chromatographic column effluent detection device
CN207727013U (en) * 2017-12-21 2018-08-14 泰州迈博太科药业有限公司 A kind of antibody purification efflux automatic collecting device
CN210906083U (en) * 2019-11-02 2020-07-03 巴彦淖尔市金盛汇化工有限公司 Quantitative feeding device of methanesulfonic acid

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
滕晓梅: "免疫比浊法用于全自动生化分析仪中产生钩状效应的防范", 《国际检验医学杂志》, vol. 36, no. 12, 30 June 2015 (2015-06-30), pages 1649 - 1650 *
龙开平等: "《医学基础综合实验教程》", 31 January 2018, 中国科学技术出版社, pages: 81 *

Similar Documents

Publication Publication Date Title
CN205352808U (en) Online enrichment and separation device of trace silver in sea water
JP2002506989A (en) Electronic device for dispensing fluid in precise small volumes
CN202185265U (en) Medicament solution automatic preparation device for dosing facility
CN104321634A (en) Particle dispensing apparatus and method
US4009005A (en) Apparatus for radioimmunoassay with regeneration of immunadsorbent
CN108918901B (en) Multichannel flow path structure of biochemical multi-component concentration online analyzer
CN107354093B (en) Cell preparation apparatus
CN111965277A (en) Automatic collection device for antibody purification effluent liquid
CN206378411U (en) A kind of calibration system in online analytical instrument
CN208334379U (en) Sample analyser
CN211086040U (en) Flow cytometer liquid path system based on differential pressure sampling
CN111467989A (en) Novel circulating dynamic online mixer
CN108845056A (en) A kind of on-line automatic pretreating device of aflatoxin based on flow path switching
EP0236928A2 (en) Dilution apparatus and method
CN202330373U (en) Automatic dilution device for ion chromatography solution
CN201732083U (en) Ion chromatography leacheate preparation device
CN216747039U (en) Full-automatic sample pretreatment device
CN104535371B (en) Automatic sampling and filtering device capable of measuring nitration and denitrification rates
CN106324266A (en) Flow path control system and method of automatic analyzer
CN211677234U (en) Novel circulating dynamic online mixer
CN112444619B (en) Impedance method counting device and blood cell analyzer
CN114166609A (en) Full-automatic sample pretreatment device
CN212459561U (en) Double-channel ion chromatography detection system and sample injector with series double-valve structure
CN209613676U (en) A kind of full-automatic enzyme-linked immunoassay instrument washes station
CN205958588U (en) Automatic flow path system of analysis appearance

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20201120

RJ01 Rejection of invention patent application after publication