CN102225248B - Sealing assembly of chromatography column - Google Patents
Sealing assembly of chromatography column Download PDFInfo
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- CN102225248B CN102225248B CN 201110076114 CN201110076114A CN102225248B CN 102225248 B CN102225248 B CN 102225248B CN 201110076114 CN201110076114 CN 201110076114 CN 201110076114 A CN201110076114 A CN 201110076114A CN 102225248 B CN102225248 B CN 102225248B
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Abstract
The invention discloses a sealing assembly of a chromatography column. The sealing assembly is utilized for sealing an interspace between an adapter of a chromatography column and the inner wall of a cylindrical housing. The sealing assembly comprises a distributor plate, a back plate of the distributor, a hold-down plate, an elastic device, one or more gear devices and a screw located at the axial position of the cylindrical housing. Through a rotation process on the one or more gear devices, the rotation of the hold-down plate can be realized and a relative distance of the distributor plateand the back plate thereof is changed, thus the elastic device is compressed to contact with the inner wall of the cylindrical housing to avoid the leakage of liquid or particles in a chromatography column.
Description
Technical field
The present invention relates to a kind of black box of chromatographic column, especially, the invention provides for the black box between adapter and the chromatographic column inwall.
Background technology
The advantage of chromatography (Chromatography) technology in separation field is well-known, can be used for from complex mixture, effectively isolating target product, remove impurity, the purification of end product or the displacement of product solution etc. in the object, chromatographic technique has the characteristics of high separating efficiency, can under the relatively gentle condition of condition, separate temperature-sensitive or the easy product of inactivation, such as protein, polysaccharide and polypeptide etc.Chromatographic column is the key that realizes chromatography, chromatographic column is generally by shell cylindraceous, the top assembly of cylinder-like shell, the base assemblies of cylinder-like shell, between shell cylindraceous two ends, be used for trapped particles and fluid and adjustable for height adapter etc., the top of cylinder-like shell is provided with fluid intake and corresponding valve etc., the bottom of cylinder-like shell is provided with fluid distribution means, particle device for trapping and fluid issuing and corresponding valve etc., the adapter height of cylinder-like shell inside is adjustable and be provided be used to the device that fluid well-distributing is distributed and particle device for trapping etc.
The sealing that use or plant-scale chromatographic column of traditional laboratory is mainly by plate distributor, plate distributor backboard, the rotatable realizations such as cushion rubber that are positioned at stock and the elastic organic material composition of inside screw or periphery.Height by the screw rod adjustable adapter, plate distributor is provided with fluid distribution means and is used for the screen cloth of trapped particles, and the distributor backboard contacts with solid or hollow stock, moves by rotary-top stock promotion plate distributor backboard, compress the distributor backboard, produce sealing effectiveness.
Traditional chromatographic column black box Main Problems is: traditional encapsulating method is when the relative distance of adjusting between plate distributor and the plate distributor backboard, need rotation to be positioned at inside screw or the peripheral stock of chromatographic column cylinder shaft core position, this stock has objectively increased to be made the material that chromatographic column need to use and has improved the chromatographic column manufacturing cost, simultaneously, when the relative distance that changes between plate distributor and the plate distributor backboard, spacing between plate distributor outer and the plate distributor backboard outer is inhomogeneous, easily causes partially sealed tight and cause liquid or particle in the chromatographic column to leak.The sealing device of tradition chromatographic column is in dismounting, inconvenient when cleaning and installing, and process is loaded down with trivial details.
Problem for traditional chromatography column sealing assembly existence, it is a kind of improved for the sealing between adapter in the chromatographic column and the chromatographic column inwall that the object of the invention is to provide, improve the sealing effectiveness of chromatographic column, improve the chromatographic column black box of the separating property of chromatography process.
Summary of the invention
The invention provides a kind of assembly for the chromatographic column sealing, can between chromatographic column cylinder-like shell inwall and adapter, produce sealing effectiveness by means of the sealing assembly, effectively avoid the leakage of the interior liquid of chromatographic column and particle.
Described black box is comprised of the screw rod that is positioned at the cylinder-like shell shaft core position, plate distributor, plate distributor backboard, compression plate, elastic element and gear device.
The screw rod that is positioned at the cylinder-like shell shaft core position passes in the middle of the cover plate of cylinder-like shell top, is provided with screw thread in the middle of the cover plate, and its screw thread mates with screw rod, but the position by rotary screw up-down adjustment screw rod.
Plate distributor is connected with the lower end that is positioned at cylinder-like shell shaft core position screw rod, the plate distributor bottom is provided with fluid distribution means, the fluid that enters chromatographic column is evenly distributed on the chromatographic column cross section, the bottom surface assembling screen cloth of plate distributor passes through upwards escaping of plate distributor to prevent particle, is provided with the hole that fluid is passed through in the middle of the plate distributor; Bucking ladder is arranged at plate distributor top, changes the compressible elastomeric device of the distance between plate distributor and the plate distributor backboard.
The plate distributor backboard is positioned at the top of plate distributor, the centre is provided with circular hole, the screw rod that is positioned at the cylinder-like shell shaft core position passes from the circular hole in the middle of the plate distributor backboard, and can slide up and down along the screw rod that is positioned at the cylinder-like shell shaft core position, flange is arranged at plate distributor backboard bottom, dwindles the compressible elastomeric device of the up and down relative distance between plate distributor and the plate distributor backboard.
Compression plate be diameter less than the round tooth wheel disc of cylinder-like shell internal diameter, is provided with circular hole in the middle of the compression plate, establish internal thread in the circular hole.The screw rod that is positioned at the cylinder-like shell shaft core position passes from the circular hole in the middle of the compression plate, the internal thread of compression plate and the coupling of the screw thread on the screw rod, can it be moved up and down by rotatably compressing dish, and move up and down by it and to change relative distance between plate distributor and the plate distributor backboard.
The compressible elastomeric device is between plate distributor and plate distributor backboard, cause the compressible elastomeric device to deform by the relative distance that changes between plate distributor and the plate distributor backboard, so that the compressible elastomeric device contacts with the inwall of cylinder-like shell, produce sealing effectiveness.
The gear device is positioned at the top of plate distributor backboard, and gear shaft is fixed on the plate distributor backboard.Rotation by the gear device can cause that compression plate rotates and and then cause compression plate to move up and down along the screw rod direction, thereby change the relative distance between plate distributor and the plate distributor backboard, compress or loosen elastic element.
The material of the screw rod of cylinder-like shell shaft core position, plate distributor, plate distributor backboard and compression plate can be plastic material or metal material.Plastic material can be high density polyethylene (HDPE), high density poly propylene, polytetrafluoroethylene (PTFE), polyformaldehyde, polyether-ether-ketone, Merlon or acrylonitrile-butadiene-styrene (ABS); Metal material can be 314,316 or the stainless steel such as 316L.
The screw rod of cylinder-like shell shaft core position, plate distributor, plate distributor backboard and compression plate and gear device are stable in the 0-1MPa scope, and be stable in the temperature range of 0-50 degree.
The arrangement mode of each element is that plate distributor backboard and gear device all are positioned at plate distributor top in the black box, and compression plate is positioned at plate distributor backboard top, and elastic element is between plate distributor backboard and plate distributor.
Preferably, the upper gear device that is positioned at the plate distributor backboard can be one, also can be a plurality of.
Preferably, the rotation of gear device can be carried out manual adjustments by the screw rod that can pass the cylinder-like shell top blind flange, also can drive by motor it is rotated.
Preferably, between plate distributor and the plate distributor backboard pin can be set, when rotatably compressing dish, make between plate distributor and the plate distributor backboard not relatively rotate.
Preferably, compression plate be height less than the circular device of diameter, the centre is provided with threaded hole.
Characteristics of the present invention are to provide a kind of black box of chromatographic column, by rotatably compressing the relative distance between dish change plate distributor and the plate distributor backboard, can dwindle the relative distance between plate distributor and the plate distributor backboard after certain orientation rotatably compresses dish, elasticity of compression device makes it contact with cylinder-like shell and produces sealing effectiveness.The advantage of the black box that this invention provides is can not change in the seal process distance between chromatographic column internal granular layer and the plate distributor.Compression plate is simple in structure, and is easily manufactured.Spacing in seal process between plate distributor outer and the plate distributor backboard outer is even, is difficult for causing partially sealed tight and cause that liquid or particle in the chromatographic column leak.
Description of drawings
Be illustrated as the present invention by reference the following drawings and do more detailed explanation
Fig. 1 is the sectional view of chromatographic column, has shown black box of the present invention among the figure.
Fig. 2 is black box enlarged drawing of the present invention.Wherein 1 is plate distributor, the 2nd, and the plate distributor backboard, the 3rd, compression plate, the 4th, the gear device, the 5th, elastic element, the 6th, be positioned at the screw rod of cylinder-like shell shaft core position.
Fig. 3 has shown compression plate and gear device relative position.Wherein 7 is cross sections of cylinder-like shell, the 3rd, and compression plate, the 4th, gear device.
Claims (5)
1. chromatographic column black box, be used for the sealing between chromatographic column adapter and the cylinder-like shell inwall, it is characterized in that the chromatographic column black box is by plate distributor, the plate distributor backboard, compression plate, elastic element, gear device and the screw rod composition that is positioned at the cylinder-like shell shaft core position, the arrangement mode of each element is that the plate distributor backboard is positioned at plate distributor top in the assembly, compression plate is positioned at plate distributor backboard top, elastic element is between plate distributor backboard and plate distributor, compression plate is that diameter is less than the round tooth wheel disc of cylinder-like shell internal diameter, be provided with circular hole in the middle of the compression plate, internal thread is set in the circular hole, the internal thread of compression plate and the screw thread coupling that is positioned on the screw rod of cylinder-like shell shaft core position, can realize that by the swing pinion device compression plate rotates, and the relative distance between change plate distributor and the plate distributor backboard, and then elasticity of compression device make its with the cylinder-like shell inwall between contact, avoid in the chromatographic column liquid or particle to leak.
2. assembly according to claim 1, plate distributor backboard, compression plate, plate distributor and elastic element are stable in the 0-1MPa scope in the assembly, and be stable in the temperature range of 0-50 degree.
3. assembly according to claim 1, compression plate can be mobile on the screw rod that is positioned at the cylinder-like shell shaft core position by rotation.
4. assembly according to claim 1, the rotation of compression plate can change the relative distance between plate distributor and the plate distributor backboard, compresses or unclamps elastic element.
5. assembly according to claim 1, the gear device cooperates with the gear of compression plate, and the rotation of transmitting gear device can cause that compression plate rotates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110076114 CN102225248B (en) | 2011-03-29 | 2011-03-29 | Sealing assembly of chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110076114 CN102225248B (en) | 2011-03-29 | 2011-03-29 | Sealing assembly of chromatography column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102225248A CN102225248A (en) | 2011-10-26 |
| CN102225248B true CN102225248B (en) | 2013-10-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110076114 Active CN102225248B (en) | 2011-03-29 | 2011-03-29 | Sealing assembly of chromatography column |
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| CN (1) | CN102225248B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102836572B (en) * | 2012-09-17 | 2014-11-05 | 於红军 | Double-cavity dynamic compression column for chromatographic separation |
| EP2931400B1 (en) * | 2012-12-14 | 2021-07-07 | Cytiva BioProcess R&D AB | Method for cleaning of packed bed chromatography columns |
| DE202016000500U1 (en) * | 2016-01-26 | 2016-03-15 | Dr. Albin Maisch High Performance LC GmbH | LC column with permanent static-axial compression (SAC) |
| CN107149795B (en) * | 2017-06-27 | 2024-02-13 | 江苏汉邦科技股份有限公司 | Chromatographic column sealing assembly |
| CN114768302B (en) * | 2022-06-14 | 2022-09-16 | 山东惠民中联生物科技有限公司 | Chromatography device in preparation process of paste bird repellent |
Citations (7)
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|---|---|---|---|---|
| US4888112A (en) * | 1986-11-06 | 1989-12-19 | Labomatic Gmbh | Chromatographic column |
| US5423982A (en) * | 1994-05-31 | 1995-06-13 | Biosepra Inc. | Liquid chromatography column adapted for in situ chemical sterilization |
| CN1764833A (en) * | 2003-03-21 | 2006-04-26 | 米利波尔公司 | Chromatographic column sealing device |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| EP2204225A1 (en) * | 2008-12-22 | 2010-07-07 | SynChro GmbH | Column reactor |
| CN201676559U (en) * | 2010-03-03 | 2010-12-22 | 青岛康原药业有限公司 | Separation and extraction chromatographic column |
| CN201676560U (en) * | 2010-03-03 | 2010-12-22 | 青岛康原药业有限公司 | Novel chromatographic column |
-
2011
- 2011-03-29 CN CN 201110076114 patent/CN102225248B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888112A (en) * | 1986-11-06 | 1989-12-19 | Labomatic Gmbh | Chromatographic column |
| US5423982A (en) * | 1994-05-31 | 1995-06-13 | Biosepra Inc. | Liquid chromatography column adapted for in situ chemical sterilization |
| CN1764833A (en) * | 2003-03-21 | 2006-04-26 | 米利波尔公司 | Chromatographic column sealing device |
| CN200948371Y (en) * | 2006-07-07 | 2007-09-19 | 陈开明 | Axial pressure adding type preparation and chromatography pole |
| EP2204225A1 (en) * | 2008-12-22 | 2010-07-07 | SynChro GmbH | Column reactor |
| CN201676559U (en) * | 2010-03-03 | 2010-12-22 | 青岛康原药业有限公司 | Separation and extraction chromatographic column |
| CN201676560U (en) * | 2010-03-03 | 2010-12-22 | 青岛康原药业有限公司 | Novel chromatographic column |
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| CN102225248A (en) | 2011-10-26 |
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