CN104399282B - A kind of manual chromatographic column - Google Patents
A kind of manual chromatographic column Download PDFInfo
- Publication number
- CN104399282B CN104399282B CN201410395977.8A CN201410395977A CN104399282B CN 104399282 B CN104399282 B CN 104399282B CN 201410395977 A CN201410395977 A CN 201410395977A CN 104399282 B CN104399282 B CN 104399282B
- Authority
- CN
- China
- Prior art keywords
- column
- column cap
- screw
- lifting rod
- manual chromatographic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
A kind of manual chromatographic column, including 1 glass tube column part 2 of column cap part and substrate portion 3 can be produced.The post head point includes the column cap flange that can be produced, column cap support member, leading screw, lifting rod, small rotary wheel, rotating wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor.The manual chromatographic column is it is characterized in that the column cap part can be screwed out around the rotary screw on the upper decorative cover of the column jecket part；The column cap part also includes the lifting rod in the leading screw inner chamber, and the lifting rod can be realized being lifted and be declined by small rotary wheel；Especially, the lifting of the lifting rod and decline can promote the sealing hold-down ring of the column cap part to realize compression and the release of sealing ring, and then realize sealing and the depressurization of column cap.Sealed column cap due to only sealing ring just being compressed when column cap moves to specified location, therefore can greatly prolong the physical life of sealing ring.
Description
Technical field
The present invention relates to a kind of manual chromatographic column, and in particular to a kind of column cap can turn around the rotary screw on decorative cover on column jecket
Go out；Column cap adopts O-shaped seal form；Drive lever and column cap hold-down ring to compress above-mentioned O-ring by lifting rod, realize column cap
Sealing；Upper chromatography mouth is passed from leading screw side；Filling, unloads post, and easy to clean, corner or spot as yet untouched by a clean-up campaign is few, for the manual of industry chromatography
Chromatographic column.
Background of invention
Chromatographic column is the main body in gel chromatography technology, typically uses glass tube or lucite tube.Under chromatographic column screen cloth
Dead volume should be little as far as possible, if the dead volume under screen cloth is big, the possibility re-mixed between separation component is just big, its
As a result it is to affect wash-out peak shape, conditions of streaking occurs, reduce definition.When being precisely separated, it is desirable to which dead volume is no more than total
The 1/1000 of bed volume.
In prior art, manual chromatographic column is with its cheap price and good performance still in tomography devices market share
Suitable share.However, technically still there is a following difficult problem in the manual chromatographic column for commercial Application：1st, screen cloth is assembled and is torn open
Unload relatively difficult；2nd, there is corner or spot as yet untouched by a clean-up campaign between screen cloth and distributor；3rd, the sealing between column cap and column jecket adopts profile seal
Cast formula, increases process costs；4th, the general volume of industrial manual chromatographic column is larger, and column cap is heavier, pulls down column cap and tubing string is entered
Row unloads glue or cleaning quite inconvenience.
To solve an above-mentioned difficult problem, the present invention provides a kind of manual chromatographic column, by the unique design sealed to column cap, preferably
O-shaped seal form, short chromatography stream and the column cap form that can be produced can solve an above-mentioned difficult problem as far as possible.
The content of the invention
The present invention provides a kind of manual chromatographic column, and column cap can be produced around rotary screw, unique column cap design of Sealing Structure,
Preferred O-shaped seal form, as far as possible short chromatography stream can efficiently reduce corner or spot as yet untouched by a clean-up campaign, convenient operation and cleaning.
The technical solution used in the present invention is as follows：
A kind of manual chromatographic column, including column cap part, glass tube column part and substrate portion can be produced.
The post head point that produces is including the column cap flange that can be produced, column cap support member, leading screw, lifting rod, small-sized rotation
Runner, rotating wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor etc.；
The column cap flange for producing adopts mode connects for screw with the column cap support member, has tetrafluoro to pad under column cap flange
Lubrication between existing contact surface.
The leading screw is trapezoidal screw, and the trapezoidal screw can be by the rotation of feed screw nut realization longitudinal direction lifting；
The trapezoidal screw inner chamber has the lifting rod, and the lifting rod realizes lifting rod described by the rotation of the small rotary wheel
Lifting in leading screw.
The lower end of the lifting rod is connected with lever, and the other end of the lever is connected with the column cap hold-down ring, described
There is column cap sealing ring under column cap hold-down ring.
One end of the lever can be elevated with the upper shifting of the lifting rod, at the same the other end of the lever then under
Drop, and then drive the column cap hold-down ring to move down, the compression of sealing ring realizes sealing.Column cap sealing release action with it is above-mentioned
Process is contrary.
The upper chromatography mouth is passed from leading screw side, shortens system path and corner or spot as yet untouched by a clean-up campaign.
The glass tube column part includes lucite tube, upper and lower decorative cover, screw rod etc., and glass tube column can have different size
Internal diameter：100mm, 150mm, 200mm, 250mm, 300mm, 450mm etc..
The lucite column jecket can be individual layer or bilayer.
The upper and lower decorative cover is assembled to the two ends of the glass tube column, and by the tie-rod.
The screw rod has 4-6 roots, positioned at glass tube column periphery, uniform to assemble.
The substrate portion includes substrate, and lower distributor, lower screen cloth chromatograph mouth, supporting leg etc..
Maximum outside diameter of the substrate more than the upper and lower decorative cover.
On the substrate, both are fastenedly connected the lower distributor by screw.
The lower screen cloth is located on lower distributor, under glass tube column, and three is sealed by U-shaped sealing ring.
The foot-supporting frame has 4, and castor can be installed by foot, facilitates the movement of equipment.
The manual chromatographic column, including column cap part, glass tube column part and substrate portion can be produced.The post head subpackage
Include the column cap flange that can be produced, column cap support member, leading screw, lifting rod, small rotary wheel, rotating wheel, column cap hold-down ring, leading screw spiral shell
Mother, upper chromatography mouth, distributor etc.；The glass tube column part includes lucite tube, upper and lower decorative cover, screw rod etc.；The substrate
Part includes substrate, distributor, supporting leg etc..The manual chromatographic column is it is characterized in that the column cap part can be around the column jecket portion
The rotary screw on upper decorative cover for dividing is screwed out；The column cap part also includes the lifting rod in the leading screw inner chamber, the lifting
Bar can be realized being lifted and be declined by small rotary wheel；Especially, the lifting of the lifting rod and decline can promote the column cap
Partial sealing hold-down ring realizes compression and the release of sealing ring, and then realizes sealing and the depressurization of column cap.The upper strata
Analysis mouth is passed from leading screw side, is shortened stream, is reduced corner or spot as yet untouched by a clean-up campaign.
Advantage of the invention is that the column cap can be produced around the rotary screw on upper decorative cover；Column cap sealing can rely on small-sized
Rotating wheel is lifted or declines lifting rod to realize；Upper chromatography pipeline is passed from leading screw side, and stream is short, reduces corner or spot as yet untouched by a clean-up campaign.
The invention will be further elaborated with reference to the accompanying drawings and detailed description.
Description of the drawings
Fig. 1 show entirety graphics of the invention
Fig. 2 show rotary flange part of the present invention explosive view
Fig. 3 show column cap part of the present invention explosive view
Fig. 4 show glass column tube portion explosive view of the present invention
Fig. 5 show substrate portion explosive view of the present invention
Fig. 6 show column cap sectional view of the present invention
Specific embodiment
A kind of manual chromatographic column as shown in figs 1 to 6, specifically：1. column cap part；2. glass column tube portion；3. substrate
Part；4. driving wheel；5. feed screw nut fixture；6. phenyl tetrafluoride pad 1；7. phenyl tetrafluoride pad 2；8. support member；9. rotary screw；10. revolve
Turned flange；11. handles；12. taper knobs；13. feed screw nuts；15. feed screw nut baffle plates；16. top covers；17. small-sized wheels；18. 4
Fluorine pad 2；19. support rings；20. leading screws；21. lifting rods；22. pipelines；23. column cap glands；The anti-rotating part of 24. compressions, 25. handles,
26. compressing tablets；27. phenyl tetrafluoride pads 3；28. lock-on connections；29. holding tube rings；Distributor backboard on 30.；31. column cap hold-down rings；32. thick sticks
Bar；33. distributors；34. distributor friction tops；35. distributor pressure rings；36. upper screen cloths；37.U type circles；38.O types circle 1；39.
Upper screen cloth outer shroud；Decorative cover on 40.；41. phenyl tetrafluoride pads 4；42. column jecket pull bars；43. column jeckets；44.O types circle 2；45. times decorative covers；Under 46.
Screen cloth sealing ring；47. lower screen cloths；48. times distributors；49. substrates；50. times chromatography mouths；51. supporting legs；52. castors.
Specifically, lower chromatography mouth is closed, column cap is produced around rotary screw, trip bolt is tightened；Afterwards filler is poured into
Glass tube column, then column cap is gone to into origin-location, screw is tightened, and handle is rotated by operating personnel, column cap moves down into specified location,
Small-sized wheel is rotated, lifting rod is lifted, sealing ring is compressed, rotating driver moves down column cap, filling compaction completes to fill post behaviour
Make.
Specifically, lower chromatography mouth is opened, is ready to connect liquid device, chromatographic solution is pumped into from the health mouth of pipe, Jing fillers are separated
Afterwards, target substance is connect into liquid device, completes purification step.
Specifically, if filler need to be changed, column cap rotary screw is produced, tightens trip bolt, and by filler in glass tube
Break up recovery, cleaning glass column jecket etc., then filler again, cycle column cap, again by filling compaction.
The present invention is different from prior art, and column cap can be produced around the rotary screw on upper decorative cover；Column cap sealing can rely on little
Type rotating wheel is lifted or declines lifting rod to realize；Upper chromatography pipeline is passed from leading screw side, and stream is short, reduces corner or spot as yet untouched by a clean-up campaign.
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention is not thereby limited, it is every using this
Equivalent structure or equivalent flow conversion that bright specification and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (16)
1. a kind of manual chromatographic column, including the column cap part that can be produced, glass tube column part and substrate portion, it is characterised in that institute
State post head point can be produced including the column cap flange that can be produced, column cap support member, leading screw, lifting rod, small rotary wheel, rotation
Wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor, the leading screw are trapezoidal screw, and the trapezoidal screw can pass through
The rotation of the feed screw nut realizes that longitudinal direction lifts；The trapezoidal screw inner chamber has the lifting rod, and the lifting rod is by described
Lifting of the lifting rod in the leading screw is realized in the rotation of small rotary wheel.
2. a kind of manual chromatographic column according to claim 1, it is characterised in that the column cap flange for producing with it is described
Column cap support member adopts mode connects for screw, has phenyl tetrafluoride pad to realize the lubrication between contact surface under column cap flange.
3. a kind of manual chromatographic column according to claim 1, it is characterised in that the lower end of the lifting rod is connected with lever,
The other end of the lever is connected with the column cap hold-down ring, has column cap sealing ring under the column cap hold-down ring.
4. a kind of manual chromatographic column according to claim 3, one end of lever described in its feature can be with the lifting rod
Upper shifting and it is elevated, while the other end of the lever then declines, and then drive the column cap hold-down ring to move down, the pressure of sealing ring
Tightly, realize sealing.
5. a kind of manual chromatographic column according to claim 1, it is characterised in that action one end that column cap sealing is released can be with
The moving down for lifting rod and be lowered by, while the other end of the lever is then upper moving, and then drive on the column cap hold-down ring
Move, sealing ring unclamps, realize deblocking.
6. a kind of manual chromatographic column according to claim 1, it is characterised in that the upper chromatography mouth is passed from leading screw side,
Shorten system path and corner or spot as yet untouched by a clean-up campaign.
7. a kind of manual chromatographic column according to claim 3, it is characterised in that the column cap hold-down ring, is tied using split
Structure, is connected with each other by screw.
8. a kind of manual chromatographic column according to claim 1, it is characterised in that the glass tube column part includes organic glass
Glass pipe, upper and lower decorative cover, screw rod, glass tube column can have different size internal diameter：100mm, 150mm, 200mm, 250mm, 300mm,
450mm。
9. a kind of manual chromatographic column according to claim 8, it is characterised in that the lucite tubing string can be individual layer or
Person is double-deck.
10. a kind of manual chromatographic column according to claim 8, it is characterised in that the upper and lower decorative cover is assembled to the glass
The two ends of tubing string, and by the tie-rod.
11. a kind of manual chromatographic columns according to claim 8, it is characterised in that the screw rod has 4-6 roots, positioned at the glass
Glass tubing string periphery, it is uniform to assemble.
12. a kind of manual chromatographic columns according to claim 8, it is characterised in that the substrate portion includes substrate, lower point
Orchestration, lower screen cloth chromatograph mouth, foot-supporting frame.
13. a kind of manual chromatographic column according to claim 12, it is characterised in that the substrate is more than the upper and lower decorative cover
Maximum outside diameter.
A kind of 14. manual chromatographic columns according to claim 12, it is characterised in that the lower distributor the substrate it
On, both are fastenedly connected by screw.
A kind of 15. manual chromatographic columns according to claim 12, it is characterised in that the lower screen cloth be located at lower distributor it
On, under glass tube column, three is sealed by U-shaped sealing ring.
16. a kind of manual chromatographic columns according to claim 12, it is characterised in that the foot-supporting frame has 4, and foot can pacify
Dress castor, facilitates the movement of equipment.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410395977.8A CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
| PCT/CN2015/080970 WO2016023396A1 (en) | 2014-08-13 | 2015-06-08 | Manual chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410395977.8A CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104399282A CN104399282A (en) | 2015-03-11 |
| CN104399282B true CN104399282B (en) | 2017-04-05 |
Family
ID=52636986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410395977.8A Expired - Fee Related CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN104399282B (en) |
| WO (1) | WO2016023396A1 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104399282B (en) * | 2014-08-13 | 2017-04-05 | 源创精科生物科技(长沙)有限公司 | A kind of manual chromatographic column |
| CN113082770A (en) * | 2019-12-23 | 2021-07-09 | 江苏汉邦科技有限公司 | Be applied to rotary device of chromatography column |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1446611A (en) * | 2003-03-28 | 2003-10-08 | 东北林业大学 | Chromatography device with negative pressure |
| CN1531652A (en) * | 2001-07-04 | 2004-09-22 | ��Ĭɭ�����ѧ����˾ | Endpiece for chromatography column |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7041216B2 (en) * | 2003-02-24 | 2006-05-09 | Millipore Corporation | Chromatography column |
| GB2419830B (en) * | 2004-11-02 | 2009-11-04 | Amersham Biosciences Ab | Chromatography column with rotatable sections |
| CN202128946U (en) * | 2011-01-27 | 2012-02-01 | 北京清博华科技有限公司 | Dynamic axial compressed chromatographic column and column installation system |
| CN203408494U (en) * | 2013-08-02 | 2014-01-29 | 荣捷生物工程（苏州）有限公司 | Manual chromatographic column device |
| CN104399282B (en) * | 2014-08-13 | 2017-04-05 | 源创精科生物科技(长沙)有限公司 | A kind of manual chromatographic column |
| CN204502468U (en) * | 2014-08-13 | 2015-07-29 | 源创精科生物科技(长沙)有限公司 | A kind of manual chromatographic column |
-
2014
- 2014-08-13 CN CN201410395977.8A patent/CN104399282B/en not_active Expired - Fee Related
-
2015
- 2015-06-08 WO PCT/CN2015/080970 patent/WO2016023396A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1531652A (en) * | 2001-07-04 | 2004-09-22 | ��Ĭɭ�����ѧ����˾ | Endpiece for chromatography column |
| CN1446611A (en) * | 2003-03-28 | 2003-10-08 | 东北林业大学 | Chromatography device with negative pressure |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016023396A1 (en) | 2016-02-18 |
| CN104399282A (en) | 2015-03-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104399282B (en) | A kind of manual chromatographic column | |
| WO2022007493A1 (en) | Mixing device for antihypertensive captopril capsule | |
| CN209548740U (en) | A kind of Chinese traditional medicine dispensing Medical liquor extraction apparatus | |
| CN204502468U (en) | A kind of manual chromatographic column | |
| CN106726578A (en) | A kind of electrodynamic type Chinese medicine dreg pressurizing unit | |
| CN108891064B (en) | Pharmacy is with automatic film clamp | |
| CN208073396U (en) | A kind of well mouth sealing device being easily installed | |
| CN114582739A (en) | Integrated circuit semiconductor chip packaging equipment and packaging method thereof | |
| CN204246880U (en) | Intermittent screw thread formula sampling chromatographic column | |
| CN109090623A (en) | A kind of middle-size and small-size fruits and vegetables juice is vertical to squeeze separator | |
| CN102225248B (en) | Sealing assembly of chromatography column | |
| JP5630299B2 (en) | Liquid chromatography apparatus and packing method for packing material | |
| CN203408494U (en) | Manual chromatographic column device | |
| CN202909984U (en) | Spray head lifting mechanism for case cleaning device | |
| CN205884610U (en) | Red bayberry wine makes cranberry juice production facility system of usefulness | |
| CN205652419U (en) | Asparagus packing compressor | |
| CN102038609A (en) | Piston type traditional Chinese medicine tank | |
| CN207545990U (en) | A kind of mechanical filter convenient for cleaning | |
| CN208852487U (en) | A kind of Rosa roxburghii Tratt class refined filtration funnel fixed device | |
| CN115678682B (en) | Camellia oil concentration and extraction equipment | |
| CN106422417A (en) | Novel solid-phase extraction column capable of performing suction under negative pressure | |
| CN104324523B (en) | Complete thread formula sampling chromatographic column | |
| CN219156830U (en) | Improved distillation extraction device for traditional Chinese medicine plant volatile oil | |
| CN206297129U (en) | A kind of double screw extruder with novel evacuated seat | |
| CN206473865U (en) | A kind of efficient pressurizing chromatographic column of use for laboratory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Li Yuntian Document name: Notification of Passing Examination on Formalities |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent for invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 410000 Hunan province Changsha Wenxuan High-tech Development Zone, Road No. 27 Building No. 101 Yuyuan Lu Valley A2 Applicant after: Biotechnology (Changsha) Co., Ltd of Yuan Chuanjing section Address before: 410000 Hunan city high tech Development Zone Changsha Changsha Wenxuan Road No. 27 Building No. 101 Yuyuan Lu Valley A2 Applicant before: CHANGSHA YUANCHUANG JINGKE BIOTECHNOLOGY CO., LTD. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: CHANGSHA YUANCHUANG JINGKE BIOTECHNOLOGY CO., LTD. TO: YUANCHUANG JINGKE BIO-TECHNOLOGY (CHANGSHA) CO., LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170405 Termination date: 20180813 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |