CN104399282A - Hand-operated chromatography column - Google Patents
Hand-operated chromatography column Download PDFInfo
- Publication number
- CN104399282A CN104399282A CN201410395977.8A CN201410395977A CN104399282A CN 104399282 A CN104399282 A CN 104399282A CN 201410395977 A CN201410395977 A CN 201410395977A CN 104399282 A CN104399282 A CN 104399282A
- Authority
- CN
- China
- Prior art keywords
- column
- chromatographic column
- screw
- column cap
- manual chromatographic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
Abstract
A hand-operated chromatography column contains a roll-out column head part 1, a glass tubular column part 2 and a substrate part 3. The column head part contains a roll-out column head flange, a column head support, a lead screw, a draw bar, a small revolving wheel, a revolving wheel, a column head hold-down ring, a lead screw nut, an upper chromatographic opening and a distributor. The hand-operated chromatography column is characterized in that the column head part can be rolled out around a rotation screw on an upper decorative cover of the tubular column part; the column head part also contains the draw bar in a lead screw cavity; the draw bar can ascend and descend through the small revolving wheel; and especially, by ascending and descending of the draw bar, seal ring compaction and loosening of a seal hold-down ring of the column head part can be promoted, so as to realize sealing and sealing relief of a column head. The seal ring is compacted to seal the column head only when the column head moves to a specified location. Thus, service life of the seal ring can be prolonged greatly.
Description
Technical field
The present invention relates to a kind of manual chromatographic column, being specifically related to a kind of column cap can produce by the rotary screw on column jecket on decorative cover; Column cap adopts O type seal form; Drive lever and column cap hold-down ring to compress above-mentioned O type circle by lifting rod, realize column cap sealing; Upper chromatography mouth passes from leading screw side; Filling, unload post, easy to clean, corner or spot as yet untouched by a clean-up campaign is few, for the manual chromatographic column of industrial chromatography.
background of invention
Chromatographic column is the main body in gel chromatography technology, generally with glass tube or lucite tube.Dead volume under chromatographic column screen cloth should be little as much as possible, if the dead volume under screen cloth is large, the possibility again mixed between separated component is just large, consequently affects wash-out peak shape, occurs conditions of streaking, reduces definition.When being accurately separated, require that dead volume can not exceed 1/1000 of total bed volume.
In prior art, manual chromatographic column with its cheap price and good performance still in the suitable share of tomography devices market share.But still there is a following difficult problem technically in the manual chromatographic column for commercial Application: 1, screen cloth assembles and dismantles more difficult; 2, there is corner or spot as yet untouched by a clean-up campaign between screen cloth and distributor; 3, the sealing between column cap and column jecket adopts fixing ring form, increases process costs; 4, the general volume of industrial manual chromatographic column is comparatively large, and column cap is heavier, pulls down column cap and unloads glue or cleaning quite inconvenience to tubing string.
For solving an above-mentioned difficult problem, the invention provides a kind of manual chromatographic column, by the unique design sealed column cap, preferred O type seal form, as far as possible short chromatography stream and the column cap form that can produce can solve an above-mentioned difficult problem.
Summary of the invention
The invention provides a kind of manual chromatographic column, column cap can produce around rotary screw, unique column cap design of Sealing Structure, preferred O type seal form, and as far as possible short chromatography stream, can reduce corner or spot as yet untouched by a clean-up campaign, handled easily and cleaning effectively.
The technical solution used in the present invention is as follows:
A kind of manual chromatographic column, comprises and can produce column cap part, glass tube column part and substrate portion.
The described post head that produces divides the column cap flange comprising and can produce, column cap support member, leading screw, lifting rod, and small rotary is taken turns, swiveling wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor etc.;
The described column cap flange produced adopts screw to be connected with described column cap support member, has the lubrication that phenyl tetrafluoride pad realizes between contact surface under column cap flange.
Described leading screw is trapezoidal screw, and described trapezoidal screw realizes longitudinally lifting by the rotation of described feed screw nut; Described trapezoidal screw inner chamber has described lifting rod, and the rotation that described lifting rod is taken turns by described small rotary realizes the lifting of lifting rod in described leading screw.
The lower end of described lifting rod is connected with lever, and the other end of described lever is connected with described column cap hold-down ring, has column cap sealing ring under described column cap hold-down ring.
One end of described lever can be moved with described lifting rod upper and promoted, and the other end of described lever then declines simultaneously, and then drives described column cap hold-down ring to move down, the compression of sealing ring, realizes sealing.The action that column cap sealing is removed is contrary with said process.
Described upper chromatography mouth passes from leading screw side, shortens system path and corner or spot as yet untouched by a clean-up campaign.
Described glass tube column part comprises lucite tube, upper and lower decorative cover, screw rod etc., and glass tube column can have different size internal diameter: 100mm, 150mm, 200mm, 250mm, 300mm, 450mm etc.
But described lucite column jecket individual layer or bilayer.
Described upper and lower decorative cover is assembled to the two ends of described glass tube column, and by described tie-rod.
Described screw rod has 4-6 root, is positioned at described glass tube column peripheral, uniform assembling.
Described substrate portion comprises substrate, lower distributor, lower screen cloth, chromatography mouth, supporting leg etc.
Described substrate is greater than the maximum outside diameter of described upper and lower decorative cover.
Described lower distributor is on described substrate, and both connect by screw fastening.
Described lower screen cloth is positioned on lower distributor, and under glass tube column, three is by U-shaped sealing ring sealing.
Described foot-supporting frame has 4, and castor can be installed by foot, facilitates the movement of equipment.
This manual chromatographic column, comprises and can produce column cap part, glass tube column part and substrate portion.Described post head divides the column cap flange comprising and can produce, column cap support member, leading screw, lifting rod, and small rotary is taken turns, swiveling wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor etc.; Described glass tube column part comprises lucite tube, upper and lower decorative cover, screw rod etc.; Described substrate portion comprises substrate, distributor, supporting leg etc.Described manual chromatographic column is characterized in that described column cap part can screw out by the rotary screw on the upper decorative cover of described column jecket part; Described column cap part also comprises the lifting rod in described leading screw inner chamber, and described lifting rod realizes promoting and declining by small rotary wheel; Especially, lifting and the decline of described lifting rod can impel the sealing hold-down ring of described column cap part realize the compression of sealing ring and unclamp, and then realize sealing and the depressurization of column cap.Described upper chromatography mouth passes from leading screw side, shortens stream, decreases corner or spot as yet untouched by a clean-up campaign.
Advantage of the present invention is that described column cap can produce by the rotary screw on upper decorative cover; Small rotary can be relied on to take turns lifting for column cap sealing or decline lifting rod realizes; Upper chromatography pipeline passes from leading screw side, and stream is short, reduces corner or spot as yet untouched by a clean-up campaign.
Below in conjunction with the drawings and specific embodiments, the invention will be further elaborated.
Accompanying drawing explanation
Figure 1 shows that the overall graphics of the present invention
Figure 2 shows that rotary flange part explosive view of the present invention
Figure 3 shows that column cap part explosive view of the present invention
Figure 4 shows that glass column tube portion explosive view of the present invention
Figure 5 shows that substrate portion explosive view of the present invention
Figure 6 shows that column cap sectional view of the present invention
Detailed description of the invention
The manual chromatographic column of one as shown in figs 1 to 6, particularly: 1. column cap part; 2. glass column tube portion; 3. substrate portion; 4. driving wheel; 5. feed screw nut fixture; 6. phenyl tetrafluoride pad 1; 7. phenyl tetrafluoride pad 2; 8. support member; 9. rotary screw; 10. rotary flange; 11. handles; 12. taper knobs; 13. feed screw nuts; 15. feed screw nut baffle plates; 16. top covers; 17. small-sizedly take turns; 18. phenyl tetrafluoride pads 2; 19. support rings; 20. leading screws; 21. lifting rods; 22. pipelines; 23. column cap glands; 24. compress anti-rotating part, 25. handles, 26. compressing tablets; 27. phenyl tetrafluoride pads 3; 28. lock-on connections; 29. holding tube rings; 30. upper distributor backboard; 31. column cap hold-down rings; 32. levers; 33. distributors; 34. distributor friction tops; 35. distributor pressure rings; 36. upper screen cloths; 37.U type circle; 38.O type circle 1; 39. upper screen cloth outer shrouds; Decorative cover on 40.; 41. phenyl tetrafluoride pads 4; 42. column jecket pull bars; 43. column jeckets; 44.O type circle 2; 45. times decorative covers; 46. lower screen cloth sealing rings; 47. lower screen cloths; 48. times distributors; 49. substrates; 50. times chromatography mouths; 51. supporting legs; 52. castors.
Particularly, close lower chromatography mouth, column cap is produced around rotary screw, tightens trip bolt; Pour filler into glass tube column afterwards, then forward column cap to origin-location, tighten screw, by operating personnel's turning handle, column cap moves down into assigned address, rotates small-sized wheel, promotes lifting rod, is compressed by sealing ring, rotating driver, moves down column cap, by filling compaction, completes dress column operation.
Particularly, open lower chromatography mouth, be ready to connect liquid device, chromatographic solution is pumped into from the health mouth of pipe, after filler is separated, target substance is connect liquid device, completes purification step.
Particularly, if need change filler, produced by column cap rotary screw, tighten trip bolt, and filler in glass tube is broken up recovery, cleaning glass column jecket etc., then filler again, cycle column cap, again by filling compaction.
The present invention is different from prior art, and column cap can produce by the rotary screw on upper decorative cover; Small rotary can be relied on to take turns lifting for column cap sealing or decline lifting rod realizes; Upper chromatography pipeline passes from leading screw side, and stream is short, reduces corner or spot as yet untouched by a clean-up campaign.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention and accompanying drawing content to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (18)
1. a manual chromatographic column, comprises the column cap part that can produce, glass tube column part and substrate portion.
2. the manual chromatographic column of one according to claim 1, can produce post head described in it is characterized in that and divide the column cap flange comprising and can produce, column cap support member, leading screw, lifting rod, small rotary is taken turns, swiveling wheel, column cap hold-down ring, feed screw nut, upper chromatography mouth, distributor etc.
3. the manual chromatographic column of one according to claim 2, is characterized in that the described column cap flange that can produce adopts screw to be connected with described column cap support member, has the lubrication that phenyl tetrafluoride pad realizes between contact surface under column cap flange.
4. the manual chromatographic column of one according to claim 2, is characterized in that described leading screw is trapezoidal screw, and described trapezoidal screw realizes longitudinally lifting by the rotation of described feed screw nut; Described trapezoidal screw inner chamber has described lifting rod, and the rotation that described lifting rod is taken turns by described small rotary realizes the lifting of lifting rod in described leading screw.
5. the manual chromatographic column of one according to claim 2, is characterized in that the lower end of described lifting rod is connected with lever, and the other end of described lever is connected with described column cap hold-down ring, has column cap sealing ring under described column cap hold-down ring.
6. the manual chromatographic column of one according to claim 5, the one end of lever described in its feature can be moved with described lifting rod upper and promoted, and the other end of described lever then declines simultaneously, and then drives described column cap hold-down ring to move down, the compression of sealing ring, realizes sealing.
7. the manual chromatographic column of one according to claim 1, is characterized in that the action that column cap sealing is removed is contrary with described claim 6.
8. the manual chromatographic column of one according to claim 2, is characterized in that described upper chromatography mouth passes from leading screw side, shortens system path and corner or spot as yet untouched by a clean-up campaign.
9. the manual chromatographic column of one according to claim 5, is characterized in that described column cap hold-down ring, adopts Split type structure, is interconnected by screw.
10. the manual chromatographic column of one according to claim 1, is characterized in that described glass tube column part comprises lucite tube, upper and lower decorative cover, screw rod etc., glass tube column can have different size internal diameter: 100mm, 150mm, 200mm, 250mm, 300mm, 450mm etc.
The manual chromatographic column of 11. one according to claim 8, but it is characterized in that described lucite column jecket individual layer or bilayer.
The manual chromatographic column of 12. one according to claim 8, is characterized in that described upper and lower decorative cover is assembled to the two ends of described glass tube column, and by described tie-rod.
The manual chromatographic column of 13. one according to claim 8, is characterized in that described screw rod has 4-6 root, is positioned at described glass tube column peripheral, uniform assembling.
The manual chromatographic column of 14. one according to claim 1, is characterized in that described substrate portion comprises substrate, lower distributor, lower screen cloth, chromatography mouth, supporting leg etc.
The manual chromatographic column of 15. one according to claim 12, is characterized in that described substrate is greater than the maximum outside diameter of described upper and lower decorative cover.
The manual chromatographic column of 16. one according to claim 12, is characterized in that described lower distributor is on described substrate, and both connect by screw fastening.
The manual chromatographic column of 17. one according to claim 12, is characterized in that described lower screen cloth is positioned on lower distributor, and under glass tube column, three is by U-shaped sealing ring sealing.
The manual chromatographic column of 18. one according to claim 12, it is characterized in that described foot-supporting frame has 4, castor can be installed by foot, facilitates the movement of equipment.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410395977.8A CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
| PCT/CN2015/080970 WO2016023396A1 (en) | 2014-08-13 | 2015-06-08 | Manual chromatography column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410395977.8A CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104399282A true CN104399282A (en) | 2015-03-11 |
| CN104399282B CN104399282B (en) | 2017-04-05 |
Family
ID=52636986
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410395977.8A Expired - Fee Related CN104399282B (en) | 2014-08-13 | 2014-08-13 | A kind of manual chromatographic column |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN104399282B (en) |
| WO (1) | WO2016023396A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016023396A1 (en) * | 2014-08-13 | 2016-02-18 | 源创精科生物科技(长沙)有限公司 | Manual chromatography column |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113082770A (en) * | 2019-12-23 | 2021-07-09 | 江苏汉邦科技有限公司 | Be applied to rotary device of chromatography column |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1446611A (en) * | 2003-03-28 | 2003-10-08 | 东北林业大学 | Chromatography device with negative pressure |
| CN1531652A (en) * | 2001-07-04 | 2004-09-22 | ��Ĭɭ�����ѧ����˾ | Endpiece for chromatography column |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
| US20090039023A1 (en) * | 2004-11-02 | 2009-02-12 | Ge Healthcare Bio-Sciences Ab | Chromatography column |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7041216B2 (en) * | 2003-02-24 | 2006-05-09 | Millipore Corporation | Chromatography column |
| CN202128946U (en) * | 2011-01-27 | 2012-02-01 | 北京清博华科技有限公司 | Dynamic axial compressed chromatographic column and column installation system |
| CN203408494U (en) * | 2013-08-02 | 2014-01-29 | 荣捷生物工程（苏州）有限公司 | Manual chromatographic column device |
| CN104399282B (en) * | 2014-08-13 | 2017-04-05 | 源创精科生物科技(长沙)有限公司 | A kind of manual chromatographic column |
| CN204502468U (en) * | 2014-08-13 | 2015-07-29 | 源创精科生物科技(长沙)有限公司 | A kind of manual chromatographic column |
-
2014
- 2014-08-13 CN CN201410395977.8A patent/CN104399282B/en not_active Expired - Fee Related
-
2015
- 2015-06-08 WO PCT/CN2015/080970 patent/WO2016023396A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1531652A (en) * | 2001-07-04 | 2004-09-22 | ��Ĭɭ�����ѧ����˾ | Endpiece for chromatography column |
| US20050173324A1 (en) * | 2001-07-04 | 2005-08-11 | Owe Salven | Endpiece for a chromatography column |
| CN1446611A (en) * | 2003-03-28 | 2003-10-08 | 东北林业大学 | Chromatography device with negative pressure |
| US20090039023A1 (en) * | 2004-11-02 | 2009-02-12 | Ge Healthcare Bio-Sciences Ab | Chromatography column |
| CN2815464Y (en) * | 2005-08-27 | 2006-09-13 | 王柯 | Liquid-phase chromatographic fractionation column |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016023396A1 (en) * | 2014-08-13 | 2016-02-18 | 源创精科生物科技(长沙)有限公司 | Manual chromatography column |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016023396A1 (en) | 2016-02-18 |
| CN104399282B (en) | 2017-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209548740U (en) | A kind of Chinese traditional medicine dispensing Medical liquor extraction apparatus | |
| CN204502468U (en) | A kind of manual chromatographic column | |
| CN104399282A (en) | Hand-operated chromatography column | |
| CN203874524U (en) | Vertical filter press | |
| CN108891064B (en) | Pharmacy is with automatic film clamp | |
| CN109011773A (en) | A kind of dynamic circulation peony seed oil filtering device | |
| CN108102789A (en) | A kind of lemon extraction process squeezing process processing equipment | |
| CN114582739A (en) | Integrated circuit semiconductor chip packaging equipment and packaging method thereof | |
| CN201453742U (en) | Disposable blood injecting device | |
| CN203408494U (en) | Manual chromatographic column device | |
| CN205884610U (en) | Red bayberry wine makes cranberry juice production facility system of usefulness | |
| CN204246880U (en) | Intermittent screw thread formula sampling chromatographic column | |
| CN207970971U (en) | Magnetic drawer | |
| CN207827007U (en) | A kind of chemical industry extremely toxic substance high-efficiency collecting device | |
| CN208049452U (en) | Column chromatography amplification technique equipment | |
| CN208049015U (en) | A kind of electrodynamic type Chinese medicine dreg pressurizing unit | |
| CN207333694U (en) | A kind of fluid pressure type plunger pump multifunctional containment gland | |
| CN207698335U (en) | A kind of foot-operated hand cleanser bottle | |
| CN109468190B (en) | Multi-functional soak device for rice wine | |
| CN208372150U (en) | A kind of prepared slices of Chinese crude drugs Quick-pressing formula water logging permeator | |
| CN207278439U (en) | A kind of water drawing device | |
| CN105693113A (en) | Convenient sealing device | |
| CN201651304U (en) | Conveniently replaced shower pipe sealing structure for washing tower | |
| CN102038609A (en) | Piston type traditional Chinese medicine tank | |
| CN204275568U (en) | Complete thread formula sampling chromatographic column |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| DD01 | Delivery of document by public notice |
Addressee: Li Yuntian Document name: Notification of Passing Examination on Formalities |
|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB02 | Change of applicant information |
Address after: 410000 Hunan province Changsha Wenxuan High-tech Development Zone, Road No. 27 Building No. 101 Yuyuan Lu Valley A2 Applicant after: Biotechnology (Changsha) Co., Ltd of Yuan Chuanjing section Address before: 410000 Hunan city high tech Development Zone Changsha Changsha Wenxuan Road No. 27 Building No. 101 Yuyuan Lu Valley A2 Applicant before: CHANGSHA YUANCHUANG JINGKE BIOTECHNOLOGY CO., LTD. |
|
| COR | Change of bibliographic data |
Free format text: CORRECT: APPLICANT; FROM: CHANGSHA YUANCHUANG JINGKE BIOTECHNOLOGY CO., LTD. TO: YUANCHUANG JINGKE BIO-TECHNOLOGY (CHANGSHA) CO., LTD. |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170405 Termination date: 20180813 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |