US20050173324A1 - Endpiece for a chromatography column - Google Patents
Endpiece for a chromatography column Download PDFInfo
- Publication number
- US20050173324A1 US20050173324A1 US10/482,711 US48271103A US2005173324A1 US 20050173324 A1 US20050173324 A1 US 20050173324A1 US 48271103 A US48271103 A US 48271103A US 2005173324 A1 US2005173324 A1 US 2005173324A1
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- US
- United States
- Prior art keywords
- net
- net support
- welding
- liquid transfer
- transfer system
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000004587 chromatography analysis Methods 0.000 title description 4
- 238000003466 welding Methods 0.000 claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 18
- 238000009826 distribution Methods 0.000 claims description 28
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 239000010935 stainless steel Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 9
- 238000012856 packing Methods 0.000 description 4
- 238000007789 sealing Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 1
- 238000005219 brazing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000002861 polymer material Substances 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 238000005476 soldering Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N30/6017—Fluid distributors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6004—Construction of the column end pieces
- G01N30/603—Construction of the column end pieces retaining the stationary phase, e.g. Frits
Definitions
- the present invention relates to chromatography columns. More specifically, the invention relates to a net for large-scale chromatography columns.
- Chromatography columns often contain a net to prevent media from escaping from the end cells which are used for radially distributing the fluid flowing in from a central inlet at one end of the column and for radially collecting the fluid for subsequent outputting from a central outlet at the opposite end of the column.
- These nets extend across substantially the whole internal diameter of the column.
- the nets have been made of polymer materials and have been attached (with a supporting backing plate if required) to the central inlet resp. central outlet by means of screws or bolts.
- the screws or bolts are mounted with their heads on the side of the net facing away from the inlet, resp. outlet, and they pass through suitably sized holes in the net and are screwed into suitably threaded axial holes in the wall of the central inlet resp. outlet.
- nets have been attached by being mounted on a central inlet or outlet tubular port provided with an internal thread and a stop flange positioned on the outside of the port at a distance from the end where the net is to be mounted which is less than the thickness of the net.
- the net is then positioned against the flange with the end of the port extending into a central opening in the net.
- the net is held in place by a threaded end piece which has a tubular portion provided with a thread adapted to mate with the thread on the inside of the port, and a flange at one end which pushes the net against the flange on the port when the end piece is screwed up into the port.
- fluid system is intended to designate the apparatus in which liquid is either introduced to or withdrawn from a cell at a zone approximately transverse the direction of flow through the cell.
- cell is intended to include the terms “vessel” and “column”, as well as any other structure utilised by practitioners of the separation arts, to effect a separation and/or extraction of components from an admixture by bringing the admixture into contact with solid or liquid exchange media, above referred to as the packing.
- Cross-sectional zone (or region) refers to a region within a cell bounded by cross sections of the cell-oriented transverse (typically approximately normal) the longitudinal direction of flow through the cell.
- “Longitudinal direction of flow” refers to the direction of flow from an inlet towards an outlet within a cell. “Longitudinal” is used consistently to designate the dominant flow path of fluid through a cell without regard to direction.
- Flow connection system refers to a system of channels or paths that connect two points in a fluid circuit. While the skilled person realises that columns may be arranged in many different orientations, for the sake of convenience and clarity of description, it will be assumed that the upper end of the column is the inlet end while the bottom end is the outlet end.
- “Distribution system” refers to structure through which fluids are introduced to a cell and “collection system” refers to structure used to withdraw fluids from a cell, in each instance from a cross-sectional zone.
- the object of the invention is to provide a new way to support the net in a large scale fluid system, which overcomes the drawbacks of the prior art systems. This is achieved by the liquid transfer system as defined in claim 1 .
- Another advantage is that the proposed liquid transfer system is that no parts oif the system interfere with the bed.
- Still a further advantage is that the proposed liquid transfer system reduces the risk for sanitary problems, due to the simple design comprising few interconnected elements.
- Still a further advantage is that the proposed liquid transfer system simple and therefore cheap to produce.
- FIG. 1 shows schematically a conventional separator system in cross-section.
- FIG. 2 shows schematically a second prior art separator system.
- FIGS. 3 a - 3 c show arrangements for fixation of the net to the net support according to the invention.
- FIG. 4 shows another arrangement for fixation of the net to the net support according to the invention.
- FIG. 5 shows a first embodiment of the present invention.
- FIG. 6 shows an alternative embodiment of the present invention.
- FIG. 7 shows another alternative embodiment of the present invention.
- FIG. 1 shows schematically a conventional separator system 100 in cross-section.
- the separator system 100 is essentially rotationally symmetric and it comprises sidewalls 110 , and a distribution system 130 .
- the distribution system 130 comprises an inlet 140 , and a distribution arrangement 150 , for example radially extending ribs, for evenly distributing the mobile phase over the cross-section of the column 100 at the inlet end, and a similar arrangement at the outlet end (not shown) for evenly collecting the mobile phase at the outlet end from the cross-section of the column.
- the distribution system 130 is terminated by a net support 160 , which for instance may be a perforated plate.
- a fine net 170 (mesh) or an equivalent filter material is arranged below the net support 160 facing the packing.
- the net 170 and the net support 160 are attached to the distribution system 130 by screws 180 , and in that they are clamped around their outer circumferences between the sidewalls 110 and the distribution system 130 .
- sealing means such as O-rings 190 , 200 are provided at each screw 180 and around the perimeter of the system respectively.
- FIG. 2 shows schematically another prior art separator system 300 .
- This system differs from the system above in that it comprises a central nozzle 310 that can be used to inject slurry or cleaning fluid into the column.
- the net support 160 and the net 170 therefore each has a central hole with a diameter that is slightly larger than the outer diameter of the central nozzle 310 .
- the central nozzle 310 is further used to centrally fixate the net support 160 and the net 170 by a flange 320 on the outer perimeter of the central nozzle 310 , and a second flange 330 on the portion of the central nozzle that extends into the bed. Due to this a lesser number of screws 180 is needed, depending on the size of the system, but the problem relating to screws interfering with the bed still remain.
- the present invention avoids these problems by integrally joining the net 170 with the net support 160 , such that the net is rigidly fixed to the net support 160 , creating an integrated net arrangement
- the net support 160 of a material, which is compatible with the material of the net 170 , they may for example be joined together by welding.
- FIGS. 3 a - 3 c show examples of arrangements of welding-holes 350 providing a sufficient fixation of the net.
- the welding holes are arranged as slots along a circle that is concentric with the net support 160 .
- the welding holes are arranged as radially extending slots, and in FIG. 3 c the slots of FIG. 3 a are substituted by circular holes.
- the lower edge 400 of the welding-holes 350 whereto the net 170 is to be welded may be provided with a small welding-protrusion 410 as is shown in FIG. 4 .
- the welding-protrusion 410 extends in the plane of the net support 160 towards the middle of the welding hole 350 , and acts as a material source in the welding process whereby unwanted recesses may be avoided at the edge 400 .
- the net support 160 should further be adapted to the flow characteristics of the system. However, it may be necessary to make the holes in which the net is to be welded relatively wide as it is difficult to reach down and weld if the hole is to narrow. If the net support 160 has a thickness of about 8 mm a suitable width may be in the magnitude of 20 mm.
- the welding-protrusion 410 further facilitates the welding as the upper part of the welding holes is given a larger diameter. To reduce the excess volume in the welding holes 350 and to match the flow characteristics, a flow-plug 420 or the like may be provided in the hole.
- the flow-plug 420 is conveniently made of a machinable or mouldable material, e.g. plastic, and is designed such that the flow path through the welding-hole 350 is divided into one or more smaller paths, that match the flow characteristics of the system.
- the flow-plug 420 is a structure that is centred in the welding hole 350 , such that smaller paths are created between the sides of the flow-plug 420 and the walls of the welding hole 350 .
- the net support 160 is formed such that it is rigid enough to be supported only at its outer perimeter. By this all supporting screws and the like may be omitted, whereby interference with the bed is reduced to a minimum.
- a rigid net support 160 may be obtained, such as choosing a more rigid material or increasing the thickness of the material it is made from. As large scale systems may have a column diameter of 400 millimetres up to 2 meters or more, the thickness of the net support 160 have to be appropriate selected for each column diameter.
- a thickness of about 3 mm would be useful at normal flow rates, but for a 1.5 m column the thickness of the steel-plate should be 8 to 10 mm.
- a relationship between the column diameter and the thickness of a stainless steel net support 160 is about 500:3.
- the rigidity is further highly dependent on the design of the welding-hole 350 arrangement. Further, if the system is used at higher flow rates, the thickness may have to be increased.
- Such a rigid net support 160 may also be formed such that it supports the distribution arrangement 150 , such as shown in FIG. 6 .
- the distribution arrangement 150 is comprised of a disc shaped distribution element 510 and a distribution gap 520 that is terminated by the net support 160 .
- a number of spacing elements 530 are provided in between the net support 160 and the distribution arrangement 150 , which elements 530 support the distribution element 510 and defines the height of the distribution gap 520 .
- Similar spacing elements 540 are provided in between the distribution element 510 and a main body of the distribution arrangement 150 , thereby defining a distribution channel 550 .
- the spacing elements 530 , 540 may be provided separate from, or may be integrally formed on the surface on the comprised parts.
- the net support 160 is supported at its outer perimeter together with a number of support screws 610 that are evenly spaced to additionally support the net support 160 .
- no screws 610 are in direct contact with the bed, as the net support 160 is provided with threaded structures 620 and the screws 610 extends through holes in the distribution system to engage the threaded structures 620 .
- the threaded structures 620 may e.g. be threaded holes in the net support 160 or threaded structures that protrude from the upper surface of the net support 160 .
- Sleeves 630 are provided to set the right distance between the distribution arrangement 150 and the net support 160 . If the sleeves 630 are made of a material having sealing properties, e.g. a suitable plastic material, additionally sealing means may be omitted, thus achieving improved sanitary properties.
- a material having sealing properties e.g. a suitable plastic material
- Either one of the above embodiments may further be adapted for use together with a central nozzle 310 , whereby additional rigidity is achieved.
- the central nozzle 310 is integrally joined with the net 170 and the net support 160 , whereby many sanitary problems and other problems of prior art are solved.
- ring is not intended to be limited to circular means for retaining a net but is also intended to include means for retaining a net having other shapes e.g. square, rectangular etc.
- the present invention is not limited to use with metal nets and metal means for retaining a net welded together but is also applicable to plastic nets and plastic means for retaining a net that may also be welded together. While the invention has been illustrated by the use of welding to join the net and means for supporting the net together it is also conceivable to use any other inherently leak-proof means of joining the net and means for retaining a net such as soldering, brazing, gluing or the like, although welding is the preferred method as it does not introduce any impurities or unwanted substances into the column. It is also conceivable that the nets and means for retaining a net may be formed integrally, for example, by being cast, sintered or moulded in one piece.
Abstract
Description
- The present invention relates to chromatography columns. More specifically, the invention relates to a net for large-scale chromatography columns.
- Chromatography columns often contain a net to prevent media from escaping from the end cells which are used for radially distributing the fluid flowing in from a central inlet at one end of the column and for radially collecting the fluid for subsequent outputting from a central outlet at the opposite end of the column. These nets extend across substantially the whole internal diameter of the column. In the prior art the nets have been made of polymer materials and have been attached (with a supporting backing plate if required) to the central inlet resp. central outlet by means of screws or bolts. The screws or bolts are mounted with their heads on the side of the net facing away from the inlet, resp. outlet, and they pass through suitably sized holes in the net and are screwed into suitably threaded axial holes in the wall of the central inlet resp. outlet.
- Alternatively nets have been attached by being mounted on a central inlet or outlet tubular port provided with an internal thread and a stop flange positioned on the outside of the port at a distance from the end where the net is to be mounted which is less than the thickness of the net. The net is then positioned against the flange with the end of the port extending into a central opening in the net. The net is held in place by a threaded end piece which has a tubular portion provided with a thread adapted to mate with the thread on the inside of the port, and a flange at one end which pushes the net against the flange on the port when the end piece is screwed up into the port.
- One problem with existing arrangements is that it is difficult to give the net proper support, due to the flexible nature of the net. As a consequence, the net may bend if the flow of sample media through the cell is high. In fact, it is common that the net bends to such an extent that it gets plastically deformed, which is highly undesirable as it influences the characteristics of the bed and thus the flow characteristics for the sample. Therefore, the net limits the flow for such columns.
- Another problem is that the heads of the mounting screws, resp. the end pieces, project into the bed media and interfere with the packing of the bed media. This may also lead to undesirable fluid-flow characteristics at the top/bottom of the bed. The heads may also cause dead volumes, i.e. spaces where fluid circulation is slow or non-existent, where particles can collect and this makes cleaning difficult and can lead to sanitary problems.
- As used herein and in the appended claims: The term “fluid system” is intended to designate the apparatus in which liquid is either introduced to or withdrawn from a cell at a zone approximately transverse the direction of flow through the cell. The term “cell” is intended to include the terms “vessel” and “column”, as well as any other structure utilised by practitioners of the separation arts, to effect a separation and/or extraction of components from an admixture by bringing the admixture into contact with solid or liquid exchange media, above referred to as the packing. “Cross-sectional zone” (or region) refers to a region within a cell bounded by cross sections of the cell-oriented transverse (typically approximately normal) the longitudinal direction of flow through the cell. “Longitudinal direction of flow” refers to the direction of flow from an inlet towards an outlet within a cell. “Longitudinal” is used consistently to designate the dominant flow path of fluid through a cell without regard to direction. “Flow connection system” refers to a system of channels or paths that connect two points in a fluid circuit. While the skilled person realises that columns may be arranged in many different orientations, for the sake of convenience and clarity of description, it will be assumed that the upper end of the column is the inlet end while the bottom end is the outlet end. “Distribution system” refers to structure through which fluids are introduced to a cell and “collection system” refers to structure used to withdraw fluids from a cell, in each instance from a cross-sectional zone.
- The object of the invention is to provide a new way to support the net in a large scale fluid system, which overcomes the drawbacks of the prior art systems. This is achieved by the liquid transfer system as defined in claim 1.
- One advantage with such a liquid transfer system is that the net is better supported, whereby plastic deformation of the net may be avoided, and the system may be used at increased flow rates.
- Another advantage is that the proposed liquid transfer system is that no parts oif the system interfere with the bed.
- Still a further advantage is that the proposed liquid transfer system reduces the risk for sanitary problems, due to the simple design comprising few interconnected elements.
- Still a further advantage is that the proposed liquid transfer system simple and therefore cheap to produce.
- Embodiments of the invention are defined in the dependent claims.
-
FIG. 1 shows schematically a conventional separator system in cross-section. -
FIG. 2 shows schematically a second prior art separator system. -
FIGS. 3 a-3 c show arrangements for fixation of the net to the net support according to the invention. -
FIG. 4 shows another arrangement for fixation of the net to the net support according to the invention. -
FIG. 5 shows a first embodiment of the present invention. -
FIG. 6 shows an alternative embodiment of the present invention. -
FIG. 7 shows another alternative embodiment of the present invention. -
FIG. 1 shows schematically aconventional separator system 100 in cross-section. As both ends of such a system often are substantially identical and therefore often either end can be used as the inlet end while the other end is used as the outlet end, only one end is shown. Theseparator system 100 is essentially rotationally symmetric and it comprisessidewalls 110, and adistribution system 130. Thedistribution system 130 comprises aninlet 140, and adistribution arrangement 150, for example radially extending ribs, for evenly distributing the mobile phase over the cross-section of thecolumn 100 at the inlet end, and a similar arrangement at the outlet end (not shown) for evenly collecting the mobile phase at the outlet end from the cross-section of the column. Thedistribution system 130 is terminated by anet support 160, which for instance may be a perforated plate. To prevent the packing from entering thedistribution system 130, and to further enhance the distribution, a fine net 170 (mesh) or an equivalent filter material is arranged below thenet support 160 facing the packing. As been described above, the net 170 and thenet support 160 are attached to thedistribution system 130 byscrews 180, and in that they are clamped around their outer circumferences between thesidewalls 110 and thedistribution system 130. To reduce leakage, sealing means such as O-rings screw 180 and around the perimeter of the system respectively. - As mentioned above this arrangement suffers from sanitary problems, e.g. matter collecting in dead volumes, and the screws may cause undesirable fluid-flow characteristics in the bed. Furthermore, in order to achieve acceptable fixation of the net 170 a large number of
screws 180 is needed, especially for large diameter columns. -
FIG. 2 shows schematically another priorart separator system 300. This system differs from the system above in that it comprises acentral nozzle 310 that can be used to inject slurry or cleaning fluid into the column. Thenet support 160 and the net 170 therefore each has a central hole with a diameter that is slightly larger than the outer diameter of thecentral nozzle 310. Thecentral nozzle 310 is further used to centrally fixate thenet support 160 and thenet 170 by aflange 320 on the outer perimeter of thecentral nozzle 310, and asecond flange 330 on the portion of the central nozzle that extends into the bed. Due to this a lesser number ofscrews 180 is needed, depending on the size of the system, but the problem relating to screws interfering with the bed still remain. - The present invention avoids these problems by integrally joining the
net 170 with thenet support 160, such that the net is rigidly fixed to thenet support 160, creating an integrated net arrangement By forming thenet support 160 of a material, which is compatible with the material of thenet 170, they may for example be joined together by welding. - There are a number of possible ways to weld the net 170 to the
net support 160 e.g. contact welding, continuos or partial welding along alower edge 400 of a number of welding-holes 350 in thenet support 160, together with welding along the outer perimeter of the net.FIGS. 3 a-3 c show examples of arrangements of welding-holes 350 providing a sufficient fixation of the net. InFIG. 3 a the welding holes are arranged as slots along a circle that is concentric with thenet support 160. InFIG. 3 b the welding holes are arranged as radially extending slots, and inFIG. 3 c the slots ofFIG. 3 a are substituted by circular holes. If the welding-hole arrangement is made such that the holes cover a sufficiently large percent of the area of the system, no extra fluid passages are needed, i.e. the over all perforation of thenet support 160 is omitted. To achieve an optimal welded joint or seam, thelower edge 400 of the welding-holes 350 whereto the net 170 is to be welded, may be provided with a small welding-protrusion 410 as is shown inFIG. 4 . The welding-protrusion 410 extends in the plane of thenet support 160 towards the middle of thewelding hole 350, and acts as a material source in the welding process whereby unwanted recesses may be avoided at theedge 400. - One aspect that has to be addressed when designing the
net support 160 is that excess volume due to wide holes or other hollow structures should be avoided. Thenet support 160 should further be adapted to the flow characteristics of the system. However, it may be necessary to make the holes in which the net is to be welded relatively wide as it is difficult to reach down and weld if the hole is to narrow. If thenet support 160 has a thickness of about 8 mm a suitable width may be in the magnitude of 20 mm. The welding-protrusion 410 further facilitates the welding as the upper part of the welding holes is given a larger diameter. To reduce the excess volume in the welding holes 350 and to match the flow characteristics, a flow-plug 420 or the like may be provided in the hole. The flow-plug 420 is conveniently made of a machinable or mouldable material, e.g. plastic, and is designed such that the flow path through the welding-hole 350 is divided into one or more smaller paths, that match the flow characteristics of the system. Preferably, the flow-plug 420 is a structure that is centred in thewelding hole 350, such that smaller paths are created between the sides of the flow-plug 420 and the walls of thewelding hole 350. - According to a first embodiment, which is shown in
FIG. 5 , thenet support 160 is formed such that it is rigid enough to be supported only at its outer perimeter. By this all supporting screws and the like may be omitted, whereby interference with the bed is reduced to a minimum. There are several ways in which such a rigidnet support 160 may be obtained, such as choosing a more rigid material or increasing the thickness of the material it is made from. As large scale systems may have a column diameter of 400 millimetres up to 2 meters or more, the thickness of thenet support 160 have to be appropriate selected for each column diameter. If, for example, a stainless steel plate is used as thenet support 160 for a 500 mm column, a thickness of about 3 mm would be useful at normal flow rates, but for a 1.5 m column the thickness of the steel-plate should be 8 to 10 mm. According to this example, a relationship between the column diameter and the thickness of a stainless steelnet support 160 is about 500:3. However, it should be noted that the rigidity is further highly dependent on the design of the welding-hole 350 arrangement. Further, if the system is used at higher flow rates, the thickness may have to be increased. - Such a rigid
net support 160 may also be formed such that it supports thedistribution arrangement 150, such as shown inFIG. 6 . In this embodiment thedistribution arrangement 150 is comprised of a disc shapeddistribution element 510 and adistribution gap 520 that is terminated by thenet support 160. A number ofspacing elements 530 are provided in between thenet support 160 and thedistribution arrangement 150, whichelements 530 support thedistribution element 510 and defines the height of thedistribution gap 520.Similar spacing elements 540 are provided in between thedistribution element 510 and a main body of thedistribution arrangement 150, thereby defining adistribution channel 550. Thespacing elements - According to another embodiment, which is shown in
FIG. 7 , thenet support 160 is supported at its outer perimeter together with a number of support screws 610 that are evenly spaced to additionally support thenet support 160. However, in this embodiment noscrews 610 are in direct contact with the bed, as thenet support 160 is provided with threadedstructures 620 and thescrews 610 extends through holes in the distribution system to engage the threadedstructures 620. Further, due to that the net 170 is welded to thenet support 160, a relatively small number of screws are needed to achieve full support of the net 170. The threadedstructures 620 may e.g. be threaded holes in thenet support 160 or threaded structures that protrude from the upper surface of thenet support 160.Sleeves 630 are provided to set the right distance between thedistribution arrangement 150 and thenet support 160. If thesleeves 630 are made of a material having sealing properties, e.g. a suitable plastic material, additionally sealing means may be omitted, thus achieving improved sanitary properties. - Either one of the above embodiments may further be adapted for use together with a
central nozzle 310, whereby additional rigidity is achieved. In one special embodiment of this type, thecentral nozzle 310 is integrally joined with the net 170 and thenet support 160, whereby many sanitary problems and other problems of prior art are solved. - It should be noted that the present invention is also applicable to columns with non-circular cross-sections, e.g. square or rectangle cross sections, and therefore the term “ring” is not intended to be limited to circular means for retaining a net but is also intended to include means for retaining a net having other shapes e.g. square, rectangular etc.
- The present invention is not limited to use with metal nets and metal means for retaining a net welded together but is also applicable to plastic nets and plastic means for retaining a net that may also be welded together. While the invention has been illustrated by the use of welding to join the net and means for supporting the net together it is also conceivable to use any other inherently leak-proof means of joining the net and means for retaining a net such as soldering, brazing, gluing or the like, although welding is the preferred method as it does not introduce any impurities or unwanted substances into the column. It is also conceivable that the nets and means for retaining a net may be formed integrally, for example, by being cast, sintered or moulded in one piece.
Claims (11)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0116345.0A GB0116345D0 (en) | 2001-07-04 | 2001-07-04 | Chromatography column |
GB0116345.5 | 2001-07-04 | ||
PCT/GB2002/002999 WO2003005018A1 (en) | 2001-07-04 | 2002-07-01 | Endpiece for a chromatography column |
Publications (2)
Publication Number | Publication Date |
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US20050173324A1 true US20050173324A1 (en) | 2005-08-11 |
US6936166B1 US6936166B1 (en) | 2005-08-30 |
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Family Applications (1)
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US10/482,711 Expired - Fee Related US6936166B1 (en) | 2001-07-04 | 2002-07-01 | Endpiece for a chromatography column |
Country Status (10)
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US (1) | US6936166B1 (en) |
EP (1) | EP1402252A1 (en) |
JP (1) | JP2004533624A (en) |
KR (1) | KR20040017273A (en) |
CN (1) | CN1531652A (en) |
AU (1) | AU2002310548B2 (en) |
CA (1) | CA2451762A1 (en) |
GB (1) | GB0116345D0 (en) |
NZ (1) | NZ530438A (en) |
WO (1) | WO2003005018A1 (en) |
Cited By (3)
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US20120037555A1 (en) * | 2009-04-08 | 2012-02-16 | Biotage Ab | Chromatography Column |
US20130001145A1 (en) * | 2011-06-29 | 2013-01-03 | Agilent Technologies, Inc. | Micro-Machined Frit and Flow Distributor Devices for Liquid Chromatography |
CN104399282A (en) * | 2014-08-13 | 2015-03-11 | 长沙市源创精科生物技术有限公司 | Hand-operated chromatography column |
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GB0322144D0 (en) | 2003-09-23 | 2003-10-22 | Amersham Biosciences Ab | Chromatography column distribution system |
DE102004043362A1 (en) | 2004-09-08 | 2006-03-09 | Bayer Technology Services Gmbh | Liquid distributor and liquid collector for chromatography columns |
GB0614316D0 (en) * | 2006-07-19 | 2006-08-30 | Ge Healthcare Bio Sciences Ab | Axial Chromatography Columns and Methods |
JP5476384B2 (en) | 2008-08-27 | 2014-04-23 | ジーイー・ヘルスケア・バイオサイエンス・バイオプロセス・コーポレイション | System and method for producing a bed support for a chromatography column |
CA2760280A1 (en) * | 2009-05-15 | 2010-11-18 | Ge Healthcare Bio-Sciences Ab | Filter holder in a chromatography column |
GB0922426D0 (en) * | 2009-12-22 | 2010-02-03 | Ge Healthcare Bio Sciences Ab | Containers for chromatography media |
SE1000230A1 (en) * | 2010-03-12 | 2011-09-06 | Ge Healthcare Bio Sciences Ab | Chromatography column fixture |
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US9797871B2 (en) | 2010-08-05 | 2017-10-24 | Ge Healthcare Bio-Sciences Ab | Method for storage and stabilization of a target substance |
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- 2002-07-01 WO PCT/GB2002/002999 patent/WO2003005018A1/en not_active Application Discontinuation
- 2002-07-01 NZ NZ530438A patent/NZ530438A/en unknown
- 2002-07-01 AU AU2002310548A patent/AU2002310548B2/en not_active Ceased
- 2002-07-01 EP EP02735659A patent/EP1402252A1/en not_active Withdrawn
- 2002-07-01 US US10/482,711 patent/US6936166B1/en not_active Expired - Fee Related
- 2002-07-01 CA CA002451762A patent/CA2451762A1/en not_active Abandoned
- 2002-07-01 CN CNA028135253A patent/CN1531652A/en active Pending
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US9089794B2 (en) * | 2009-04-08 | 2015-07-28 | Biotage Ab | Chromatography column |
US20130001145A1 (en) * | 2011-06-29 | 2013-01-03 | Agilent Technologies, Inc. | Micro-Machined Frit and Flow Distributor Devices for Liquid Chromatography |
CN104399282A (en) * | 2014-08-13 | 2015-03-11 | 长沙市源创精科生物技术有限公司 | Hand-operated chromatography column |
Also Published As
Publication number | Publication date |
---|---|
JP2004533624A (en) | 2004-11-04 |
AU2002310548B2 (en) | 2005-09-29 |
GB0116345D0 (en) | 2001-08-29 |
EP1402252A1 (en) | 2004-03-31 |
CA2451762A1 (en) | 2003-01-16 |
WO2003005018A1 (en) | 2003-01-16 |
KR20040017273A (en) | 2004-02-26 |
CN1531652A (en) | 2004-09-22 |
NZ530438A (en) | 2005-06-24 |
US6936166B1 (en) | 2005-08-30 |
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