SE459396B - Filter fitted against gel bed in chromatographic sepn. column - Google Patents

Filter fitted against gel bed in chromatographic sepn. column

Info

Publication number
SE459396B
SE459396B SE8801030A SE8801030A SE459396B SE 459396 B SE459396 B SE 459396B SE 8801030 A SE8801030 A SE 8801030A SE 8801030 A SE8801030 A SE 8801030A SE 459396 B SE459396 B SE 459396B
Authority
SE
Sweden
Prior art keywords
ring
fabric
column
piston
filter
Prior art date
Application number
SE8801030A
Other languages
Swedish (sv)
Other versions
SE8801030D0 (en
Inventor
P Bellqvist
Original Assignee
Pharmacia Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Ab filed Critical Pharmacia Ab
Priority to SE8801030A priority Critical patent/SE459396B/en
Publication of SE8801030D0 publication Critical patent/SE8801030D0/en
Publication of SE459396B publication Critical patent/SE459396B/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
    • B01D15/122—Solid phase extraction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1801—Radial flow
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1894—Liquid-liquid chromatography, e.g. centrifugal partition chromatography or extraction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1896—Membrane chromatography or membrane adsorbers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/22—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the construction of the column
    • B01D15/222—Arrangements of modules or cassettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G01N30/603—Construction of the column end pieces retaining the stationary phase, e.g. Frits

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

The chromatographic sepn. column is filled to a specific height with a bed of sepn. gel. Against the gel bed a piston is maintained pressed by a shaft. This shaft is locked against axial displacement in a column end piece. There is a centre hole through the shaft and piston through which eluent and samples for sepn. are pumped. To protect the gel bed and also hold it in position in the column, a filter is fitted on the piston end locating against the bed. The filter comprises a woven fabric fastened in a ring, which in turn is clamped via an inner circular flange in a corresp. track in the piston. The filter fabric has a very small mesh width, e.g., less than 20 micrometres, so that the plastic pulp, e.g., polypropene, used for moulding the ring cannot penetrate through the fabric and thereby moulds the fabric firmly in the ring. The fabric is of material incompatible with that of the ring plastic pulp, e.g., polytetrafluoroethylene, and cannot be fastened in the ring by conventional adhesion or welding.

Description

459 396 2 Detta ernâs genom att väven, som har en för ringplast- massan ogenomtränglig maskvidd samt är av ett med ring- plastmassan oförenligt material, är fastgjuten i ringen via för ringplastmassan genomträngliga hål, som utformats 5 i väven längs dess periferi. This is achieved by the fabric, which has a mesh size impermeable to the ring plastic mass and is of a material incompatible with the ring plastic mass, is cast in the ring via holes permeable to the ring plastic mass, which are formed in the fabric along its periphery.

Uppfinningen beskrives närmare nedan under hänvisning till bifogade ritning på vilken den enda figuren schematiskt visar en icke skalenlig längdsektion genom ett avsnitt av en kromatografisk separationskolonns inloppsände med 10 en utföringsform av ett filter enligt uppfinningen.The invention is described in more detail below with reference to the accompanying drawing, in which the single figure schematically shows a non-scalable longitudinal section through a section of the inlet end of a chromatographic separation column with an embodiment of a filter according to the invention.

På ritningen betecknar 1 ett avsnitt av inloppsänden av en kromatografisk separationskolonn, som är fylld till en viss höjd med en bädd 2 av en kromatografisk separations- gel. Mot gelbädden 2 anligger på i och för sig känt sätt en kolv 3, som hâlles pressad mot gelbädden'2 med hjälp av ett skaft 4. Detta är i sin tur låst mot axiell för- skjutning i ett vid kolonnens l ände befintligt icke visat ändstycke. 15 Genom skaftet 4 och kolven 2 sträcker sig på i och för 20 sig känt sätt en centrumurborrning 5 för inpumpning av eluent och prov som skall separeras till gelbädden 2.In the drawing, 1 denotes a section of the inlet end of a chromatographic separation column, which is filled to a certain height with a bed 2 of a chromatographic separation gel. A piston 3 abuts against the gel bed 2 in a manner known per se, which is kept pressed against the gel bed '2 by means of a shaft 4. This in turn is locked against axial displacement in an end piece (not shown at the end of the column)). . Extending through the shaft 4 and the piston 2 in a manner known per se is a center bore 5 for pumping in eluent and samples to be separated to the gel bed 2.

För att skydda gelbädden 2 och samtidigt hålla denna på plats i kolonnen l är ett filter anbragt på den mot gel- bädden 2 anliggande änden av kolven 3. Filtret består av 25 en filterväv 6, som är infäst i en ring 7, vilken i sin tur via en inre, rundgâende fläns 8 är fastklämd i en motsvarande rundgâende ursparing 9 i kolven 3.In order to protect the gel bed 2 and at the same time hold it in place in the column 1, a filter is arranged on the end of the piston 3 abutting against the gel bed 2. The filter consists of a filter fabric 6, which is attached to a ring 7, which in its turn via an inner, circumferential flange 8 is clamped in a corresponding circumferential recess 9 in the piston 3.

Vid filtret enligt uppfinningen har filterväven 6 en Sådan liten maskvidd, t ex mindre än 20 pm, att den för 30 gjutning av ringen 7 använda plastmassan, t ex polypropen, icke kan tränga igenom filterväven 6 och därigenom gjuta fast väven 6 i ringen 7. Vidare är filterväven 6 av ett med den använda ringplastmassan oförenligt material, t ex polytetrafluoretylen, varför väven 6 icke kan infästas i 10 15 20 25 459 396 3 ringen 7 genom konventionell limning eller svetsning.In the filter according to the invention, the filter fabric 6 has such a small mesh size, for example less than 20 μm, that the plastic mass used for casting the ring 7, for example polypropylene, cannot penetrate the filter fabric 6 and thereby cast the fabric 6 in the ring 7. Furthermore, the filter fabric 6 is made of a material which is incompatible with the ring plastic mass used, for example polytetrafluoroethylene, so that the fabric 6 cannot be attached to the ring 7 by conventional gluing or welding.

För att trots detta möjliggöra fastgjutning av väven 6 i ringen 7 har enligt uppfinningen hål 10, som är till- räckligt stora för att ringplastmassan skall kunna tränga igenom dessa, utformats i väven 6 längs dess periferi.In order to nevertheless enable casting of the fabric 6 in the ring 7, according to the invention holes 10, which are sufficiently large for the annular plastic mass to be able to penetrate these, have been formed in the fabric 6 along its periphery.

Dessa hål l0 har härvid placerats så att de hamnar inuti ringen 7 vid gjutningen av denna. Väven kommer därigenom .att hållas fast i ringen 7 medelst det genom hälen 10 gående materialet.These holes 10 have in this case been placed so that they end up inside the ring 7 during the casting thereof. The fabric will thereby be held in the ring 7 by means of the material passing through the heel 10.

Planheten hos den mot gelbädden 2 anliggande ytan av väven 6 är bestämmande för hur den till kolonnen l inmatade vätskan komer att vandra genom gelbädden 2. Eftersom en linjär vätskevandring genom gelbädden 2 önskas, är det väsentligt att väven 6 är helt plan.The flatness of the surface of the fabric 6 abutting the gel bed 2 determines how the liquid fed to the column 1 will travel through the gel bed 2. Since a linear liquid migration through the gel bed 2 is desired, it is essential that the fabric 6 is completely flat.

I och med att ringen 7 krymper något efter gjutningen föreligger risk för att Väven 6 skall bukta sig i synnerhet vid ringar av relativt stor diameter. För att så icke skall ske är väven 6 enligt uppfinningen ingjuten i försträckt tillstånd. Detta åstadkommas genom att Väven 6 hålles för- sträckt under gjutningen av ringen 7. Vävens 6 försträck- ning bör härvid vara större än eller lika med ringens 7 förväntade krympning.As the ring 7 shrinks slightly after casting, there is a risk that the fabric 6 will bend, especially with rings of relatively large diameter. In order not to happen, the fabric 6 according to the invention is molded in a stretched state. This is achieved by keeping the fabric 6 pre-stretched during the casting of the ring 7. The pre-stretching of the fabric 6 should in this case be greater than or equal to the expected shrinkage of the ring 7.

Vid den visade utföringsformen antages Väven 6 vara för- sträckt snett genom den icke visade gjutformen för ringen 7.In the embodiment shown, the fabric 6 is assumed to be pre-stretched obliquely through the mold not shown for the ring 7.

Hälen 10 i Väven 6 kan härvid vara utformade i förväg eller företrädesvis utformas i samband med gjutningen av ringen 7 med hjälp av ett till gjutformen hörande, icke visat organ.The heel 10 in the fabric 6 can in this case be formed in advance or preferably be formed in connection with the casting of the ring 7 by means of a member belonging to the mold, not shown.

Claims (3)

.n 459 396 PATENTKRAV Filter för anliggning mot en gelbädd (2) i en kromato- grafisk separationskolonn (1) och innefattande en_av en plastmassa gjuten ring (7) och en i denna infäst filter- väv (6), k ä n n e t e c k n a t459 396 CLAIMS REQUIRED Filters for abutment against a gel bed (2) in a chromatographic separation column (1) and comprising a ring (7) cast from a plastic mass and a filter fabric (6) attached thereto, characterized in that 1. a v att väven (6), som har en för ringplastmassan ogenomtränglig maskvidd samt är av ett med ringplastmassan oförenligt material, är fast- gjuten i ringen (7) via för ringplastmassan genomträngliga häl (10), som utformats i väven (6) längs dess periferi.1. in that the fabric (6), which has a mesh size impermeable to the ring plastic mass and is of a material incompatible with the ring plastic mass, is cast in the ring (7) via a heel (10) permeable to the ring plastic mass, which is formed in the fabric (6) along its periphery. 2. Filter enligt kravet 1, k ä n n e t e c k n a t att väven (6) är fastgjuten i förstrâckt tillstånd. avFilter according to Claim 1, characterized in that the fabric (6) is cast in a pre-stretched state. of 3. Filter enligt kravet 2, k ä n n e t e c k n a t a v att vävens (6) försträckning är större än eller lika med ringens (7) förväntade krympning.Filter according to claim 2, characterized in that the pre-stretch of the fabric (6) is greater than or equal to the expected shrinkage of the ring (7).
SE8801030A 1988-03-21 1988-03-21 Filter fitted against gel bed in chromatographic sepn. column SE459396B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
SE8801030A SE459396B (en) 1988-03-21 1988-03-21 Filter fitted against gel bed in chromatographic sepn. column

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8801030A SE459396B (en) 1988-03-21 1988-03-21 Filter fitted against gel bed in chromatographic sepn. column

Publications (2)

Publication Number Publication Date
SE8801030D0 SE8801030D0 (en) 1988-03-21
SE459396B true SE459396B (en) 1989-07-03

Family

ID=20371767

Family Applications (1)

Application Number Title Priority Date Filing Date
SE8801030A SE459396B (en) 1988-03-21 1988-03-21 Filter fitted against gel bed in chromatographic sepn. column

Country Status (1)

Country Link
SE (1) SE459396B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2248028A (en) * 1990-09-19 1992-03-25 Amicon Ltd Improved chromatography column.
GB2248027A (en) * 1990-09-19 1992-03-25 Amicon Ltd End cell for chromatography column.
WO2002073185A1 (en) * 2001-03-09 2002-09-19 Amersham Biosciences Ab Chromatography device
WO2003005018A1 (en) * 2001-07-04 2003-01-16 Amersham Biosciences Ab Endpiece for a chromatography column

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2248028A (en) * 1990-09-19 1992-03-25 Amicon Ltd Improved chromatography column.
GB2248027A (en) * 1990-09-19 1992-03-25 Amicon Ltd End cell for chromatography column.
WO2002073185A1 (en) * 2001-03-09 2002-09-19 Amersham Biosciences Ab Chromatography device
WO2003005018A1 (en) * 2001-07-04 2003-01-16 Amersham Biosciences Ab Endpiece for a chromatography column
AU2002310548B2 (en) * 2001-07-04 2005-09-29 Ge Healthcare Bio-Sciences Ab Endpiece for a chromatography column

Also Published As

Publication number Publication date
SE8801030D0 (en) 1988-03-21

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