CN115773225B - Stem cell culture perfusion device - Google Patents
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- CN115773225B CN115773225B CN202211521312.8A CN202211521312A CN115773225B CN 115773225 B CN115773225 B CN 115773225B CN 202211521312 A CN202211521312 A CN 202211521312A CN 115773225 B CN115773225 B CN 115773225B
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 57
- 230000010412 perfusion Effects 0.000 title claims abstract description 32
- 238000004113 cell culture Methods 0.000 title claims abstract description 19
- 238000001125 extrusion Methods 0.000 claims abstract description 98
- 238000009413 insulation Methods 0.000 claims abstract description 78
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 19
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000010438 heat treatment Methods 0.000 claims abstract description 7
- 239000004964 aerogel Substances 0.000 claims description 8
- 229920001971 elastomer Polymers 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 2
- 230000002265 prevention Effects 0.000 claims description 2
- 230000000630 rising effect Effects 0.000 claims description 2
- 229920002379 silicone rubber Polymers 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 2
- 230000004083 survival effect Effects 0.000 abstract description 11
- 238000000034 method Methods 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 7
- 238000006073 displacement reaction Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000007774 longterm Effects 0.000 description 3
- 239000000741 silica gel Substances 0.000 description 3
- 229910002027 silica gel Inorganic materials 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 230000020169 heat generation Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000004336 spermatogonium Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000007779 soft material Substances 0.000 description 1
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Abstract
The invention relates to the technical field of stem cell culture, in particular to a stem cell culture perfusion device which comprises a peristaltic pump, wherein the peristaltic pump comprises a driving unit, a pump head and a hose, the pump head consists of an upper cover and a base, a roller is rotatably arranged in the base and consists of a plurality of rollers, the output end of the driving unit is connected with the roller, the hose is arranged on the roller, namely between the upper cover and the base, a heat insulation pipe is arranged in the pump head, the heat insulation pipe is hollow, and the heat insulation pipe is matched with the hose. According to the invention, the heat insulation pipe is sleeved outside the hose, the plurality of rotating rollers on the roller are firstly contacted with the heat insulation pipe, and then the hose is extruded through the heat insulation pipe, so that the first contact object of the plurality of rotating rollers is not the hose any more and is limited by the material reason of the heat insulation pipe, the temperature transfer coefficient is increased, the probability of heating up the stem cell solution in the hose due to long-time extrusion is reduced, and the survival time and the increment number of the stem cells cultivated finally are increased.
Description
Technical Field
The invention relates to the technical field of stem cell culture, in particular to a stem cell culture perfusion device.
Background
Stem cell culture is environmentally demanding, for example: factors such as sterile environment, proper temperature, proper osmotic pressure, gas environment, PH value and the like are all strict requirements; the temperature has serious influence on some stem cells, for example, the isolated and purified mouse spermatogonial stem cells are respectively cultured in vitro at the temperature of 30, 34 and 37 ℃, and the result shows that the average survival time of the cells at 34 ℃ is longest and the increment quantity is the largest.
In the perfusion equipment of stem cell culture process, peristaltic pumps are generally required to transport stem cells, the peristaltic pumps drive rollers to squeeze a hose filled with fluid through motors to transport the stem cells, the hose generally used for experimental culture is a disposable hose, the peristaltic pump hose is generally a silica gel tube, the heat conductivity coefficient of the silica gel tube is about 0.6-1.5W/(K.m), the stem cell solution needs to be in a calm environment during perfusion, and then the perfusion flow is smaller, a silica gel tube with tiny flow, namely, the tube wall is about 0.86-1mm, in the perfusion process, the rollers squeeze the hose for a long time, so that the temperature of the squeezed portion of the hose is easily increased, the temperature of the stem cell solution in the hose is influenced, for example, the isolated and purified mouse spermatogonium stem cells are 33 and 34 ℃ when entering the peristaltic pump, the stem cell solution pumped out is likely to be 36 and 37 ℃ after the rollers are rubbed and squeezed for 40min-1h, and the survival rate of the cultured stem cells is reduced, and the quantity of the cultured stem cell solution is less.
Therefore, the stem cell culture perfusion device is proposed for the problem of hose temperature rise caused by long-term friction between the hose and the roller.
Disclosure of Invention
The invention aims to provide a stem cell culture perfusion device, a layer of heat insulation pipe is sleeved outside a hose, a plurality of rotating rollers on a roller are firstly contacted with the heat insulation pipe, and then the hose is extruded through the heat insulation pipe, so that a first contact object of the rotating rollers is not the hose any more, and the heat conduction coefficient is reduced due to the fact that the heat insulation pipe is made of an aerogel hose or a rubber hose, and the probability of heating up of stem cell solution in the hose due to long-time extrusion is reduced, so that the survival time of the stem cells finally cultivated is prolonged, and the increment quantity is increased, thereby solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a stem cell culture perfusion device, comprising:
the peristaltic pump comprises a driving unit, a pump head and a hose, wherein the pump head consists of an upper cover and a base, a roller is rotatably arranged in the base and consists of a plurality of rotating rollers, the output end of the driving unit is connected with the roller, and the hose is placed on the roller of the base;
further comprises:
and the pump head is internally provided with an anti-heating component for preventing the temperature of the hose from rising in the transmission process.
Preferably, the temperature rise prevention assembly comprises a heat insulation pipe arranged in the pump head, the heat insulation pipe is attached to the hose, and the heat insulation pipe is made of aerogel hose or rubber hose with microminiature caliber.
When stem cells are irrigated, a peristaltic pump is generally used for being matched with a hose to irrigate stem cell solution, a roller is arranged in a pump head of the peristaltic pump, the roller consists of a plurality of rotating rollers, the rotating rollers are matched with the inner wall of the pump head, the hose is uniformly rotated and extruded, a pump with a larger pillow-shaped volume is formed between the two rotating rollers, and the stem cell solution in the hose is extruded and moved forwards after being uniformly rotated, so that regular perfusion is formed;
however, when the hose is circularly extruded by a plurality of rotating rollers for a long time, the temperature at the extruded part of the hose can be increased due to long-time extrusion friction heat generation, and then the temperature is transmitted to the stem cell solution in the hose through the hose, so that the temperature can be increased when the stem cell solution passes through the hose in the pump head, the temperature has a certain influence on the stem cell culture environment, for example, isolated and purified mouse spermatogonium stem cells are cultured in vitro at 30, 34 and 37 ℃ and experimental results show that the average survival time of the cells is longest at 34 ℃, the increment amount is the largest, and the average survival time of the cells is shortened and the increment amount is reduced due to the increase of the temperature;
the heat insulation pipe is arranged in the pump head, the heat insulation pipe is hollow, the heat insulation pipe can be sleeved outside the hose, a plurality of rotary rollers on the roller firstly contact with the heat insulation pipe when the hose is extruded and discharged, and then the hose is extruded through the heat insulation pipe, so that a first contact object of the rotary rollers is not the hose any more and is limited by the material reason of the heat insulation pipe, the temperature transfer coefficient is increased, the probability of heating up of stem cell solution in the hose due to long-time extrusion is reduced, and the survival time and the increment number of stem cells cultivated finally are increased; because the hose is mostly disposable consumable, the heat insulation pipe can be used for a long time, and the material value used by the heat insulation pipe can be relatively higher, so that the stability of long-term operation is ensured.
The heat conductivity of aerogel is very low, is 0.018W/(K.m) under normal atmospheric temperature state, can even reach 0.009W/(K.m) under low temperature state, and the heat conductivity of PVC hose is 0.034W/(K.m) under normal atmospheric temperature state, and the material that sets up the heat-proof pipe is aerogel hose or rubber and plastic hose, and the heat-proof pipe is sheathed outside the hose, and on the friction extrusion with the gyro wheel, the first object that changes the rod contact on the gyro wheel changes into the heat-proof pipe, because the material of heat-proof pipe is the aerogel hose, the heat conductivity is very low, the heat that the gyro wheel rubbed with it produces basically can only be attached to the heat-proof pipe surface, can't permeate to the inside hose of heat-proof pipe, then guaranteed peristaltic pump when the perfusion, can not lead to the stem cell solution in the hose to rise to the long-term friction reason of gyro wheel, and then lead to the stem cell survival rate reduction of cultivating, the condition that the increment quantity is few appears.
Preferably, the heat insulation pipe is composed of two semicircular half pipes, the two half pipes are respectively arranged on the upper cover and the base, inserting rods are arranged on one side pipe wall of the two half pipes, slots matched with the corresponding inserting rods are arranged on the other side pipe wall of the two half pipes, and each slot is an inclined slot.
The heat insulation pipe is arranged in the pump head, when the hose needs to be matched with the roller, the heat insulation pipe is required to be sleeved outside the hose, the heat insulation pipe is formed by two half pipes, the two sub pipes are respectively arranged on the upper cover and the base, an experimenter only needs to open the upper cover to place the hose in the half pipe on the base and then close the upper cover, and each slot is an inclined slot, the inserted rod can be inserted into the corresponding slot, the two half pipes can be smoothly clamped, the hose can be wrapped, the heat insulation pipe is formed by the two half pipes, the two half pipes are respectively arranged in the upper cover and the base, the slots are matched with the inserted rods, the experimenter does not need to have more steps, only the hose is required to be placed in the half pipe on the base, and then the upper cover is closed, so that the heat insulation pipe can be conveniently and rapidly sleeved outside the hose, the time consumed by the preparation action before the perfusion is reduced, and the perfusion efficiency is improved.
Preferably, the heat insulation pipe and the hose are in interference fit.
The interference fit is to enlarge and deform the hole by using elasticity of the material and sleeve the hole on the shaft, when the hole is restored, the tightening force of the shaft is generated, so that the two parts are connected, the interference fit is realized by the heat insulation pipe under the matching of the inserted link and the slot with the upper cover and the base, the upper cover and the base give two half-pipe pressures, then the inserted link and the slot are clamped together, so that the heat insulation pipe wraps the hose, when the heat insulation pipe is in a normal state, the diameter of the inner ring of the heat insulation pipe is equal to the diameter of the outer ring of the hose, the heat insulation pipe can be better attached to the hose, when the heat insulation pipe is in interference fit, the tightening force of the hose can be generated, the gap between the heat insulation pipe and the hose can be reduced, the situation of relative friction is reduced, and when the peristaltic pump is operated, the situation of heating caused by the relative friction is reduced.
Preferably, a plurality of protruding ribs for extruding the hose are uniformly arranged on the inner wall of the heat insulation pipe, and the shape of each protruding rib is a long strip with a semicircular section.
Set up the one deck thermal-insulated pipe between gyro wheel and hose, just can remove the stem cell solution forward extrusion in the hose through extrusion thermal-insulated pipe at the gyro wheel, thickness has increased, the "pillow" form volume both ends that form between two adjacent changes the roller can widen, then extrusion advancing efficiency has reduced, evenly be equipped with a plurality of protruding muscle on the thermal-insulated pipe inner wall of setting, when the gyro wheel extrusion thermal-insulated pipe, the protruding muscle extrusion hose of extruded department forms a plurality of little clearances, can reduce the space of "pillow" form volume both sides and widen, then the stem cell solution that extrudees advancing between two changes the roller increases, extrusion advancing efficiency has improved.
Preferably, an extrusion groove is formed in the upper cover, and an extrusion strip for enabling the heat insulation pipe to be tightly attached to the hose is arranged in the extrusion groove, and the extrusion strip is made of silicon rubber.
The heat insulation pipe is sleeved outside the hose, relative displacement cannot occur between the heat insulation pipe and the hose, friction exists between the heat insulation pipe and the hose if relative displacement occurs, further the hose is likely to be heated due to the fact that dry cell solution in the hose is heated due to friction heat generation, the extrusion groove is formed in the upper cover, the extrusion strip is installed in the extrusion groove, the heat insulation pipe is matched with the extrusion strip, the extrusion strip is made of soft materials and has certain extensibility and spreadability, when the upper cover is buckled with the base, the extrusion groove is filled with the extrusion strip under the elastic action of the extrusion groove and the extrusion strip, then half pipes corresponding to the heat insulation pipe are extruded towards the direction of the hose, friction force between the hose and the inner wall of the heat insulation pipe is increased, the probability that friction occurs due to the fact that the heat insulation pipe and the hose are relatively displaced when the roller runs is reduced, the fact that the hose is heated due to friction is avoided, the dry cell solution in the hose is heated is further, and the average cell survival time is shortened, and the increment quantity is reduced.
Preferably, the edges of the inner rings of the two half pipes are provided with compensation spaces for compensating extrusion gaps, and the cross sections of the compensation spaces are chamfer angles.
When two half pipes are extruded and attached, if the edge between the two half pipes is completely attached, when extrusion is carried out, extrusion force can extrude the two half pipes towards the outside of the half pipes, the similar elongation effect of the two half pipes can be achieved, then the two half pipes cannot be attached to the hose completely, friction can be generated between the half pipes and the hose when the rollers contact friction the rollers, compensation spaces are formed at the edges of inner rings of the two half pipes, the cross sections of the compensation spaces are chamfer angles, namely gaps of the two chamfer angles are reserved to be used for compensation, when the two half pipes are extruded and attached, the extrusion direction is changed due to the clearance reasons of the two chamfer angles, the half pipes are extruded and filled towards the chamfer angles, then the two half pipes and the hose are attached to the hose under the action of the extrusion groove and the chamfer angles, the phenomenon that the heat insulation pipes and the hose are in relative friction is generated is avoided, the phenomenon that the hose generates heat due to friction is generated is avoided, and stability of temperature in the hose is improved when the peristaltic pump is used for filling.
Preferably, wedge-shaped blocks for guiding the fitting direction of the extrusion strips are arranged on two sides of the inside of the extrusion groove.
Although the extrusion groove cooperates with the extrusion strip to fill the extrusion groove under the action of the elasticity of the extrusion strip, the extrusion strip can then extrude the half pipe of the corresponding heat insulation pipe towards the hose direction, but certain unordered exists in the extrusion direction, and then the extrusion strip can overflow towards the left end and the right end when extruding the corresponding half pipe, the two sides inside the extrusion groove are provided with wedge blocks, the wedge blocks play a certain guiding role, after the upper cover is closed, the extrusion strip is subjected to the extrusion limiting force of the extrusion groove, after the extrusion groove is filled, the extrusion strip extrudes towards the corresponding half pipe, and under the guiding action of the wedge blocks, the extrusion strip is given to the guide of the extrusion strip towards the center, and the extrusion strip extrudes the corresponding half pipe towards the center so that the half pipe is better attached to the hose.
Preferably, the cross-sectional shape of the heat insulating pipe is divided into a major arc shape and a minor arc shape.
The cross section of the heat insulation pipe is in two states, one is in a major arc shape, the major arc is that one half pipe wraps about 3/4 of the diameter of the outer ring of the hose under the action of the extrusion strip and the compensation chamfer angle, the other half pipe wraps the rest 1/4 of the diameter, and the top half pipe wraps most of the hose, so that the hose can be wrapped stably relatively when the roller extrudes and moves the heat insulation pipe, and the friction probability between the heat insulation pipe and the hose can be reduced; a minor arc shape is that the upper half pipe and the lower half pipe both wrap 1/2 of a hose, the wrapping stability is not good of a major arc, but when the roller rolls and extrudes, the extrusion force of the hose is relatively stable due to the fact that the half pipes respectively occupy 1/2, and the pumped stem cell solution is relatively stable, so that a positive effect is played on the follow-up culture of the stem cell solution.
Preferably, the base is provided with a through hole, a heat conducting sheet for conducting heat is arranged in the through hole, and the heat conducting sheet is attached to the half pipe on the base.
After upper cover and the base lock of pump head, form a inclosed cavity in the pump head almost, the gyro wheel is in the in-process of long-time operation in-process with thermal-insulated pipe friction, because of thermal-insulated pipe material is aerogel hose or rubber and plastic hose, the heat that the friction produced can pile up at thermal-insulated pipe surface, can produce certain heat and then the temperature in the airtight space in the pump head can rise, and the heat that the gyro wheel produced most all piles up in the gyro wheel both sides, avoid leading to the stem cell solution in the hose to rise in temperature because of the heat pile up under the airtight environment, set up the through-hole at the upper cover, and be equipped with the conducting strip in the through-hole, and the conducting strip extends outside the base, then the gyro wheel discharges outside the base through the conducting strip with the heat that the thermal-insulated pipe friction produced, reduce and appear above-mentioned condition.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the stem cell culture perfusion device, the heat insulation pipe is sleeved outside the hose, the plurality of rotating rollers on the roller wheels are firstly contacted with the heat insulation pipe, and then the hose is extruded through the heat insulation pipe, so that a first contact object of the plurality of rotating rollers is not the hose any more, and is limited by the reason that the heat insulation pipe is made of an aerogel hose or a rubber hose, the heat conductivity coefficient is reduced, the probability of heating up of stem cell solution in the hose due to long-time extrusion is reduced, and the survival time and the increment number of stem cells cultivated finally are increased.
2. According to the stem cell culture perfusion device, the heat insulation pipe is formed by the two half pipes, the two half pipes are respectively arranged in the upper cover and the base, and the two half pipes are matched with the inserting grooves and the inserting rods, so that an experimenter does not need to add more steps, only needs to put the hose into the half pipe on the base, and then closes the upper cover, the heat insulation pipe can be installed, the heat insulation pipe can be conveniently and rapidly sleeved outside the hose, the time consumed by the preparation action before perfusion is reduced, and the perfusion efficiency is improved.
3. According to the stem cell culture perfusion device, the extrusion groove is filled with the extrusion strip through the cooperation of the extrusion strip and the two half pipes, and the extrusion strip can fully fill the extrusion groove under the action of the elasticity of the extrusion groove and the extrusion strip, and then the half pipes of the corresponding heat insulation pipes are extruded towards the direction of the hose, so that the friction force between the hose and the inner wall of the heat insulation pipe is increased, and the friction probability caused by the relative displacement of the heat insulation pipes and the hose during the running of the roller is reduced; the compensation space is used for compensating, the cross section of the compensation space is a chamfer, a gap between two chamfers is reserved for compensation, when two half pipes are extruded to be combined, the extrusion direction is changed due to the gap between the two chamfers, the half pipes are extruded and filled towards the chamfer, and then the two half pipes and the hose are more combined with the hose under the action of the extrusion groove and the chamfer; and the wedge-shaped block is matched with the extrusion groove, and plays a certain guiding role to guide the extrusion strip towards the center, and the extrusion strip extrudes the corresponding half pipe towards the center, so that the half pipe is better attached to the hose.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a front partial cross-sectional view of the present invention;
FIG. 3 is an enlarged view of the invention at A of FIG. 2;
FIG. 4 is a state diagram of the extrusion strip of the present invention;
FIG. 5 is an enlarged view of the invention at B of FIG. 2;
FIG. 6 is a front perspective view of a half pipe of the present invention;
fig. 7 is a cross-sectional view of a half pipe of the present invention.
In the figure: 1. a peristaltic pump; 2. a pump head; 3. a hose; 4. a roller; 5. a heat insulating pipe; 501. a half pipe; 5011. chamfering; 502. a rod; 503. a slot; 504. protruding ribs; 6. an extrusion groove; 7. extruding the strip; 8. wedge blocks; 9. a through hole; 10. and a heat conductive sheet.
Detailed Description
Referring to fig. 1 to 7, the present invention provides a stem cell culture perfusion device, which has the following technical scheme:
embodiment one:
conditions are as follows: when the radii of the two half pipes 501 are not consistent and the radius of the upper half pipe 501 is larger than that of the lower half pipe 501;
the working process comprises the following steps: when culturing stem cells with a large influence on temperature, such as isolated and purified mouse spermatogonial stem cells, the suitable temperature is 34 degrees, and the average survival time of cells is reduced and the increment is reduced at 30 degrees and 37 degrees relative to 34 degrees, the influence on the temperature of the solution is still large during culturing.
Before the perfusion, the hose 3 in the perfusion using process is required to be put into the pump head 2, the pull rod on the upper cover is pulled to open the upper cover, then the hose 3 is taken out, the hose 3 is put into the half pipe 501 on the base at a proper position, the pull rod on the upper cover is pulled back again, the upper cover descends to be buckled with the base, the two half pipes 501 are buckled under the action of the inserting rods 502 and the inserting grooves 503, and the inserting grooves 503 are all oblique grooves, so that the inserting rods 502 can be smoothly inserted into the inserting grooves 503 to finish the preparation work before the perfusion, and then the peristaltic pump 1 is started to start the perfusion.
The peristaltic pump 1 starts to work, the output end of the driving unit drives the roller 4 to rotate, a plurality of rotating rollers on the roller 4 are in contact with the heat insulation pipe 5, the protruding ribs 504 in the heat insulation pipe 5 are matched with the inner wall of the upper cover to drive stem cell liquid in the hose 3 to squeeze forward and move, and the circulation is repeated; in the process, because the diameter of the upper half pipe 501 is larger than that of the lower half pipe 501, the thermal insulation pipe 5 and the hose 3 are in a major arc state, the extrusion strip 7 extrudes the upper half pipe 501 covered on the upper cover towards the hose 3 under the elastic action of the extrusion groove 6 and the extrusion strip 7, meanwhile, two wedge blocks 8 in the extrusion groove 6 play a guiding role, after the upper cover is closed, the extrusion strip 7 is subjected to the limited extrusion force of the extrusion groove 6, after the extrusion groove 6 is filled, the extrusion strip 7 extrudes towards the corresponding half pipe 501, and under the guiding action of the wedge blocks 8, the extrusion strip 7 guides the extrusion strip 7 towards the center, and the extrusion strip 7 extrudes the corresponding half pipe 501 towards the center, so that the half pipe 501 is better attached to the hose 3; because the inner ring edges of the two half pipes 501 are provided with the chamfers 5011, gaps of the two chamfers 5011 are reserved to be used as compensation, when the two half pipes 501 are extruded to be attached, the extrusion direction is changed due to the gaps of the two chamfers 5011, the half pipes 501 are extruded and filled towards the chamfers 5011, and then the two half pipes 501 and the hose 3 are attached to the hose 3 under the action of the extrusion groove 6 and the chamfers 5011, so that the friction force between the hose 3 and the inner wall of the heat insulation pipe is increased, and the friction probability caused by the relative displacement of the heat insulation pipe 5 and the hose 3 when the roller 4 operates is reduced.
Embodiment two:
conditions are as follows: when the radius of the two half pipes 501 is consistent;
the working process comprises the following steps: before the perfusion, the hose 3 in the perfusion using process is required to be put into the pump head 2, the pull rod on the upper cover is pulled to open the upper cover, then the hose 3 is taken out, the hose 3 is put into the half pipe 501 on the base at a proper position, the pull rod on the upper cover is pulled back again, the upper cover descends to be buckled with the base, the two half pipes 501 are buckled under the action of the inserting rods 502 and the inserting grooves 503, and the inserting grooves 503 are all oblique grooves, so that the inserting rods 502 can be smoothly inserted into the inserting grooves 503 to finish the preparation work before the perfusion, and then the peristaltic pump 1 is started to start the perfusion.
The peristaltic pump 1 starts to work, the output end of the driving unit drives the roller 4 to rotate, a plurality of rotating rollers on the roller 4 are in contact with the heat insulation pipe 5, the protruding ribs 504 in the heat insulation pipe 5 are matched with the inner wall of the upper cover to drive stem cell liquid in the hose 3 to squeeze forward, and the stem cell liquid is circulated and reciprocated.
In the process, as the radius of the two half pipes 501 is consistent, the heat insulation pipe 5 and the hose 3 are in a minor arc state, the half pipes 501 respectively occupy 1/2 in the minor arc state, the extrusion force of the extrusion bar 504 on the half pipe 501 to the hose 3 is relatively stable, the pumped stem cell solution is relatively stable, the subsequent culture of the stem cell solution plays a positive role, the extrusion bar 7 extrudes the upper half pipe 501 on the upper cover towards the hose 3 under the elastic action of the extrusion groove 6 and the extrusion bar 7, simultaneously, the two wedge blocks 8 in the extrusion groove 6 play a guiding role, after the upper cover is closed, the extrusion bar 7 is limited by the extrusion groove 6, after the extrusion groove 6 is filled, the extrusion bar 7 extrudes towards the corresponding half pipe 501, and under the guiding action of the wedge blocks 8, the extrusion bar 7 extrudes the corresponding half pipe 501 towards the center, so that the half pipe 501 is better adhered to the hose 3; because the inner ring edges of the two half pipes 501 are provided with the chamfers 5011, gaps of the two chamfers 5011 are reserved to be used as compensation, when the two half pipes 501 are extruded to be attached, the extrusion direction is changed due to the gaps of the two chamfers 5011, the half pipes 501 are extruded and filled towards the chamfers 5011, and then the two half pipes 501 and the hose 3 are attached to the hose 3 under the action of the extrusion groove 6 and the chamfers 5011, so that the friction force between the hose 3 and the inner wall of the heat insulation pipe is increased, and the friction probability caused by the relative displacement of the heat insulation pipe 5 and the hose 3 when the roller 4 operates is reduced.
Claims (3)
1. A stem cell culture perfusion device, comprising:
the peristaltic pump (1), peristaltic pump (1) is including drive unit, pump head (2) and hose (3), pump head (2) are by upper cover and base composition, the base rotation is installed gyro wheel (4), gyro wheel (4) are by a plurality of swivel rolls composition, drive unit's output is connected with gyro wheel (4), hose (3) is placed on gyro wheel (4) of base;
characterized by further comprising:
the temperature rise prevention component is arranged in the pump head (2) and is used for preventing the temperature of the hose (3) from rising in the transmission process,
the anti-heating assembly comprises a heat insulation pipe (5) arranged in the pump head (2), the heat insulation pipe (5) is attached to the hose (3), and the material of the heat insulation pipe (5) can be an aerogel hose (3) or a rubber plastic hose (3) with a microminiature caliber;
the heat insulation pipe (5) consists of half pipes (501), wherein two half pipes (501) are respectively arranged on an upper cover and a base, inserting rods (502) are arranged on one side pipe wall of each half pipe (501), inserting grooves (503) matched with the corresponding inserting rods (502) are formed in the other side pipe wall of each half pipe (501), and each inserting groove (503) is a bevel groove;
a plurality of protruding ribs (504) for extruding the hose (3) are uniformly arranged on the inner wall of the heat insulation pipe (5), and the shape of each protruding rib (504) is a semicircular strip;
an extrusion groove (6) is formed in the upper cover, an extrusion strip (7) for enabling the heat insulation pipe (5) to be tightly attached to the hose (3) is arranged in the extrusion groove (6), and the extrusion strip (7) is made of silicon rubber materials and is arranged into a long strip shape;
the edges of the inner rings of the two half pipes (501) are respectively provided with a compensation space used for compensating extrusion gaps, and the cross sections of the compensation spaces are chamfers (5011);
wedge-shaped blocks (8) for guiding the attaching direction of the extrusion strip (7) are arranged on two sides of the inside of the extrusion groove (6);
the cross section of the heat insulation pipe (5) is divided into a major arc shape and a minor arc shape.
2. The stem cell culture perfusion device of claim 1, wherein: the heat insulation pipe (5) is in interference fit with the hose (3).
3. A stem cell culture perfusion device according to claim 2, wherein: the base is provided with a through hole (9), a heat conducting sheet (10) for conducting heat is arranged in the through hole (9), and the heat conducting sheet (10) is attached to a half pipe (501) on the base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211521312.8A CN115773225B (en) | 2022-11-30 | 2022-11-30 | Stem cell culture perfusion device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211521312.8A CN115773225B (en) | 2022-11-30 | 2022-11-30 | Stem cell culture perfusion device |
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| CN115773225A CN115773225A (en) | 2023-03-10 |
| CN115773225B true CN115773225B (en) | 2023-11-03 |
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Citations (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SU1043082A1 (en) * | 1982-05-06 | 1983-09-23 | Предприятие П/Я В-8402 | Peristaltic apparatus for moving a movable object |
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| CN209243062U (en) * | 2018-10-17 | 2019-08-13 | 河南安必诺检测技术有限公司 | Culture medium auto-loading system with heating and heat preserving function |
| CN209354335U (en) * | 2018-12-07 | 2019-09-06 | 昌邑市检验检测中心 | A kind of low-lift pump applied to icp ms |
| CN212360127U (en) * | 2020-06-08 | 2021-01-15 | 上海晓圣环境测量仪器有限公司 | Peristaltic pump with adjustable speed |
| CN213540681U (en) * | 2020-10-15 | 2021-06-25 | 烟台茂恩新材料科技有限公司 | Liquid glue peristaltic pump driver heat abstractor |
| CN214305921U (en) * | 2020-12-24 | 2021-09-28 | 江门市科本空调设备有限公司 | Novel anti-aging rubber-plastic heat-preservation pipe |
| CN215595848U (en) * | 2021-10-08 | 2022-01-21 | 北京和利时智能技术有限公司 | Hose heating device and peristaltic pump |
| CN216891024U (en) * | 2022-03-18 | 2022-07-05 | 上海比昂生物医药科技有限公司 | Stem cell culture perfusion device |
| CN217123744U (en) * | 2022-03-21 | 2022-08-05 | 内蒙古富特橡塑机械有限责任公司 | Cooling device for calendering production line |
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| SU1043082A1 (en) * | 1982-05-06 | 1983-09-23 | Предприятие П/Я В-8402 | Peristaltic apparatus for moving a movable object |
| US4707315A (en) * | 1984-05-18 | 1987-11-17 | Nippon Zeon Co., Ltd. | Method of making a blood pump |
| CN102116283A (en) * | 2009-12-30 | 2011-07-06 | 天津市协达伟业电子有限公司 | Method for protecting elastic hose of peristaltic pump |
| CN201884252U (en) * | 2010-12-10 | 2011-06-29 | 深圳东江华瑞科技有限公司 | Peristaltic pump equipped with hose cooling device |
| JP2015224606A (en) * | 2014-05-28 | 2015-12-14 | 株式会社エーエスエム | Cooling structure of squeezing type pump |
| CN204041401U (en) * | 2014-06-12 | 2014-12-24 | 苏州卫水环保科技有限公司 | Peristaltic pump |
| JP2016070190A (en) * | 2014-09-30 | 2016-05-09 | ニッタ株式会社 | Adiabatic pipe |
| CN206621998U (en) * | 2017-03-31 | 2017-11-10 | 北京恒峰铭成生物科技有限公司 | A kind of stem cell conditioned medium purification devices |
| CN209243062U (en) * | 2018-10-17 | 2019-08-13 | 河南安必诺检测技术有限公司 | Culture medium auto-loading system with heating and heat preserving function |
| CN209354335U (en) * | 2018-12-07 | 2019-09-06 | 昌邑市检验检测中心 | A kind of low-lift pump applied to icp ms |
| CN212360127U (en) * | 2020-06-08 | 2021-01-15 | 上海晓圣环境测量仪器有限公司 | Peristaltic pump with adjustable speed |
| CN213540681U (en) * | 2020-10-15 | 2021-06-25 | 烟台茂恩新材料科技有限公司 | Liquid glue peristaltic pump driver heat abstractor |
| CN214305921U (en) * | 2020-12-24 | 2021-09-28 | 江门市科本空调设备有限公司 | Novel anti-aging rubber-plastic heat-preservation pipe |
| CN215595848U (en) * | 2021-10-08 | 2022-01-21 | 北京和利时智能技术有限公司 | Hose heating device and peristaltic pump |
| CN216891024U (en) * | 2022-03-18 | 2022-07-05 | 上海比昂生物医药科技有限公司 | Stem cell culture perfusion device |
| CN217123744U (en) * | 2022-03-21 | 2022-08-05 | 内蒙古富特橡塑机械有限责任公司 | Cooling device for calendering production line |
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|---|---|
| CN115773225A (en) | 2023-03-10 |
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