WO2019104945A1

WO2019104945A1 - Sampling and loading device and use method of same

Info

Publication number: WO2019104945A1
Application number: PCT/CN2018/086121
Authority: WO
Inventors: 丁建文; 李嵘
Original assignee: 爱威科技股份有限公司
Priority date: 2017-11-29
Filing date: 2018-05-09
Publication date: 2019-06-06
Also published as: CN107961822A; CN107961822B

Abstract

Provided are a sampling and loading device and a sampling and loading method using the device. The sampling and loading device comprises a tubular body (1), a sampling unit (2), and a loading and sealing unit (3). The loading and sealing unit (3) is detachably arranged at the opening of the tubular body (1) and seals the opening of the tubular body (1), so that the sampling unit (2) is accommodated in the tubular body (1). The loading and sealing unit (3) is provided with a sample loading channel (31) through which a sample can flow. At least a part of the tube wall of the tubular body (1) is deformable, so that the sample can flow out from the sample loading channel (31) from the tubular body (1) by pressing the deformable tube wall of the tubular body (1).

Description

Sample sampling device and using method thereof

The present application claims priority to Chinese Patent Application No. 200911226803.9, entitled "Sampling and Loading Device and Method of Use", filed on November 29, 2017, the entire contents of which are incorporated herein by reference. In this application.

Technical field

The present application relates to the field of testing equipment, and in particular to a sampling and loading device and a method of using the same.

Background technique

The clinical test is to examine the patient's body fluids, secretions, excretions and sheddings by visual observation, physical, chemical, instrumental or molecular biological methods, and emphasize the whole process of testing (pre-analysis, analysis, analysis). Afterwards, strict quality management measures are taken to ensure the quality of the test, thus providing valuable experimental data for the clinical and patient.

In the prior art, the detection of urine, feces, gynecology and the like is usually detected by different testing instruments. The sample can be pre-processed manually by the inspector. Usually, the collected sample is taken out and added to the diluent, mixed, etc., and it is easy to contact with external pollutants, resulting in inaccurate test results. In addition, the sample can also be pre-processed by a detection instrument equipped with an automatic sample preparation device to prepare a sample that can be detected, and the prepared sample is added to the carrier through an automatic sample application device, and the detection instrument performs the detection. However, the detection instrument with automatic sample preparation and automatic sample loading function is bulky and expensive, and is not convenient for promotion.

Therefore, how to provide a sampling and loading device capable of performing sample collection and sample processing steps, avoiding the pollution problem of manual processing, and being capable of detecting with various testing instruments is a technical problem to be solved by those skilled in the art.

Summary of the invention

In order to solve the above technical problems, a first object of the present invention is to provide a sampling and loading device; a second object of the present invention is to provide a method of using the above device. The sampling and sampling device provided by the invention can complete the sample collection and processing operations, and can directly use the sampling and sampling device provided by the invention to perform the sample loading operation when the sample is used for inspection, and has the advantages of simple structure, low cost and convenience. Promotion and use.

The technical solution provided by the present invention is as follows:

A sampling and loading device comprising a tube body, a sampling component, and a sample-loading sealing member, the sample-loading sealing member being detachably disposed at the opening of the tube body and blocking an opening of the tube body, so that the The sampling component is housed in the tube body, and the sample loading sealing component is provided with a sample loading channel for the sample to flow out, at least part of the tube wall of the tube body is deformable, and the deformable tube wall of the tube body is extruded, so that The sample in the tube flows out through the sample application channel.

Preferably, at least part of the tube wall of the tubular body is deformable in particular: at least part of the tube wall of the tubular body is made of a soft material.

Preferably, a filter member for filtering a sample is provided in the sample-loading sealing member, and the sample in the tube enters the sample channel through the filter member.

Preferably, the filter component comprises a first filter component and a second filter component,

The second filter member is disposed between the sample application channel and the first filter member, the first filter member and the second filter member enclose a filter chamber, the filter chamber and the sample loading The channels are connected;

The second filter component and the tube wall of the sample application channel enclose a sample buffer cavity, and the sample buffer cavity is in communication with the filter cavity;

The filter pore size of the first filter member is larger than the filter pore size of the second filter member, and the sample liquid remaining in the filter chamber after the sample is filtered by the first filter member and the second filter member passes the sample loading The channel flows out.

Preferably, an end of the sample sealing member away from the tubular body is provided with a spotting nozzle, the loading channel is in communication with the spotting channel of the spotting nozzle, and the spotting channel of the spotting nozzle is set as a cone shape.

Preferably, a cover is further provided, the cover being detachably coupled to the loading sealing member for shielding the spotting nozzle.

Preferably, the sampling member is detachably coupled to the loading sealing member.

Preferably, the sampling component is any one of a cotton swab, a straw, a sampling brush, a sampling spoon, and a sampling sponge.

The present invention also provides a method of using the above sampling and loading device, comprising the steps of:

a, sampling: sampling using sampling parts;

b. Dilution: the sampling part to which the sample is attached is placed in the tube body containing the diluent, and the sample sealing member is sealedly connected with the opening of the tube body;

c. preparing a sample suspension: shaking the tube body to uniformly mix the sample and the diluent to prepare a sample suspension;

d. preparing an application suspension: inverting the tube body, and filtering the sample suspension in the tube through the first filter component, wherein particles larger than the pore size of the first filter component are retained in the tube body, smaller than the pore size of the first filter component a particle suspension entering the filter chamber, filtered through the second filter member, and a particle suspension smaller than the pore size of the second filter member enters the sample buffer chamber, thereby making the pore size smaller than the first filter member And a particle suspension larger than the pore size of the second filter member is retained in the filter chamber;

e. Loading: Squeeze the deformable tube wall of the tube body, so that the suspension in the filter chamber flows out through the sample loading channel for loading.

The sampling and loading device provided by the invention can realize the collection and processing of clinical test samples. When the sampling and loading device provided by the present invention is used, the sample to be inspected is collected by using a sampling component, and then placed in the tube body. Preferably, the tube is stored with a diluent for treating the sample, and more preferably, the sample is introduced into the diluent from the sampling member by shaking the tube or the like, and uniformly mixed with the diluent to form a suspension. At the same time, since the opening of the pipe body is blocked by using the sample sealing member, the sampling component to which the sample is attached is in the space enclosed by the pipe wall of the pipe body and the sample sealing member, thereby avoiding contact with external bacteria, dust and the like. To ensure the accuracy of the test results obtained using this sample.

The sample loading sealing member is provided with a sample loading channel for the sample to flow out, that is, the sample sealing member is provided with a passage connecting the inside of the tube body to the outside, and the channel is used for the sample to flow out. When it is necessary to discharge the sample from the tube body (for example, when the processed sample is added to the test carrier for detection), the tube body is inverted, and the deformable tube wall portion of the tube body is squeezed by an external force to cause the volume of the tube body to occur. The change causes the air pressure in the tube to change, so that the sample stored in the tube body flows out from the sample loading channel on the sample sealing member for the sample to flow out for testing, and the process does not need to take out the sample in the tube for processing operation, avoiding the outside world. Environmental impact test results. When the sample in the tube body is not needed, the tube body is placed vertically and stored. Since the sample sealing member is located at the opening above the tube body, the sample in the tube body or the diluent or the like is difficult to flow out from the channel through which the sample is supplied from the sample sealing member. The sample can be stored using the sampling and loading device provided by the present invention, and can be taken out when needed, and the inverted tube can be directly loaded. Preferably, the diameter of the channel for the sample to flow out on the sample sealing member is set to be small, so that the sampling device provided by the present invention can prevent the external bacteria or dust from entering the tube from the channel when the sample loading device is placed vertically, thereby affecting the sample. The representativeness, while inverting the sample, can make the sample flow out slowly, which is convenient for accurate sample loading.

In summary, the sampling and sampling device provided by the present invention can complete the sample collection and processing operations, and can directly use the sampling and sampling device provided by the present invention to perform the sample loading operation when the sample is used for inspection, and has a simple structure. , low cost, easy to promote and use.

DRAWINGS

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings to be used in the embodiments or the prior art description will be briefly described below. Obviously, the drawings in the following description are only It is a few embodiments described in the present application, and other drawings can be obtained from those skilled in the art without any creative work.

1 is a schematic view of a sampling and loading device according to an embodiment of the present invention;

2 is a schematic exploded view of a sampling and loading device according to an embodiment of the present invention;

3 is a schematic cross-sectional view of a sampling and loading device according to an embodiment of the present invention;

4 is a schematic cross-sectional view showing a sampling component of a sampling and loading device according to an embodiment of the present invention;

LIST OF REFERENCE NUMERALS: 1-tube body; 2-sampling member; 3-loading sealing member; 31-loading channel; 32-dot nozzle; 33-cover; 3a-filter chamber; 3b-sample buffer chamber; a filter element; 41 - a first filter element; 42 - a second filter element.

Detailed ways

The technical solutions in the embodiments of the present application are clearly and completely described in the following, in which the technical solutions in the embodiments of the present application are clearly and completely described. The embodiments are only a part of the embodiments of the present application, and not all of them. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments of the present application without departing from the inventive scope shall fall within the scope of the application.

As shown in FIG. 1 to FIG. 4, an embodiment of the present invention provides a sampling and loading device, comprising a tube body 1, a sampling component 2, and a sample-loading sealing member 3, wherein the sample-loading sealing member 3 is detachably disposed at the same. Opening the opening of the pipe body 1 and blocking the opening of the pipe body 1 so that the sampling member 2 is housed in the pipe body 1, and the sample applying sealing member 3 is provided with a sampling passage for the sample to flow out. 31. At least part of the tube wall of the tube body 1 is deformable, and the deformable tube wall of the tube body 1 is squeezed to allow the sample in the tube body 1 to flow out through the sample application channel 31.

The sampling and loading device provided by the invention can realize the collection and processing of clinical test samples. When the sampling and loading device provided by the present invention is used, the sample to be inspected is collected by the sampling unit 2, and then placed in the tube 1. Preferably, the tube 1 stores a diluent for treating the sample, and more preferably, the sample is taken from the sampling member 2 into the diluent by shaking the tube 1 or the like, and is uniformly mixed with the diluent to form a suspension. At the same time, since the opening of the pipe body 1 is closed by using the sample sealing member 3, the sample member 2 to which the sample is attached is placed in the space surrounded by the pipe wall of the pipe body 1 and the sample sealing member 3, thereby avoiding contact with bacteria outside. Contaminants such as dust, to ensure the accuracy of the test results obtained using the sample.

The sample applying sealing member 3 is provided with a sample loading channel 31 for the sample to flow out, that is, the sample applying sealing member 3 is provided with a passage for communicating the inside of the tube body 1 with the outside, and the sample loading channel 31 is for the sample to flow out. When it is necessary to take out the sample from the tube 1 for detection (for example, when the processed sample is added to the detection carrier for detection), the tube body 1 is inverted, and the deformable tube wall portion of the tube body 1 is squeezed by an external force. The volume of the pipe body 1 is changed, and the air pressure in the pipe body 1 is changed, so that the sample stored in the pipe body 1 flows out from the sample loading channel 31 on the sample sealing member 3 from which the sample flows out, for detection and use. The process does not need to take out the sample in the tube 1 for processing, and avoid the external environment affecting the detection result. When the sample in the tube 1 is not needed, the tube 1 is placed vertically and stored. Since the sample sealing member 3 is located at the opening above the tube 1, the sample or the diluent or the like in the tube 1 is difficult to be loaded from the sample. The sampling channel 31 for the sample to flow out is discharged, and the sample can be stored by using the sampling and loading device provided by the present invention, and is taken out when needed, and the inverted tube body 1 can be directly loaded. Preferably, the diameter of the sample application channel 31 on the sample-loading sealing member 3 for the sample to flow out is set to be small, so that the sampled sample-feeding device provided by the present invention can prevent the external bacteria or dust from entering the pipe from the channel when placed vertically. In the body 1, it affects the representativeness of the sample, and at the same time, when the sample is inverted, the sample can be discharged slowly, which is convenient for accurate sample loading.

Preferably, at least part of the pipe wall of the pipe body 1 is deformable in particular: at least part of the pipe wall of the pipe body 1 is made of a soft material.

In the present invention, the tube body 1 is partially formed of a soft material, and the portion of the tube body 1 made of a soft material is extruded by an external force, so that the volume of the tube body 1 is changed, thereby making the tube body 1 The internal pressure changes so that the sample in the pipe body 1 flows out from the sample loading passage 31 of the sample sealing member 3. For example, part of the pipe wall is made of a soft material, or the bottom of the pipe body 1 is made of a soft material. Preferably, a part of the tube wall of the tube body 1 between the bottom and the opening is made of a soft material, and the bottom of the tube body 1 is more convenient for the sampling device provided by the present invention to be placed vertically, and at the same time, the hard The opening is easily connected to the sample sealing member 3. The soft material can be rubber, plastic or any other material suitable for sample storage.

Preferably, the filter member 3 is provided with a filter member 4 for filtering the sample, and the sample inside the tube 1 enters the sample channel 31 through the filter member 4.

The filtering component 3 is provided with a filtering component for filtering the sample. When the sampling device of the present invention is used for processing a solid sample (such as feces) or other samples to be filtered, the filtering component can filter out unnecessary components. Keep the required ingredients for inspection.

Preferably, the filter member 4 includes a first filter member 41 and a second filter member 42,

The second filter member 42 is disposed between the sample application channel 31 and the first filter member 41, and the first filter member 41 and the second filter member 42 enclose a filter chamber 3a. The cavity 3a is in communication with the sample loading channel 31;

The second filter member 42 and the tube wall of the sample loading channel 31 enclose a sample buffer chamber 3b, and the sample buffer chamber 3b is in communication with the filter chamber 3a;

The filter aperture of the first filter member 41 is larger than the filter aperture of the second filter member 42, and the sample liquid remaining in the filter chamber 3a after being filtered by the first filter member 41 and the second filter member 42 It flows out through the sample loading channel 31.

As shown in FIG. 4, the filter member 4 of the present invention may be provided in two layers, specifically a first filter member 41 and a second filter member 42, and a first filter member 41, a second filter member 42, and a sample-loading member 3 The filter chamber 3a and the sample buffer chamber 3b are formed separately, and the filter aperture of the first filter member 41 is set larger than the filter aperture of the second filter member 42. In this manner, when the sampling device provided by the present invention is inverted, the sample liquid in the tube 1 is first filtered through the first filter member 41 having a large aperture and then enters the filter chamber 3a. The first filter member 41 filters the diameter of the sample. The larger particles, after the first filtered sample liquid flows into the filter chamber 3a, the sample liquid passes through the second filter member 42 having a small aperture, and the second filter member 42 filters the smaller diameter particles in the sample liquid to make the sample smaller. The sample liquid of the particles enters into the sample buffer chamber 3b, so that the sample liquid in the filter chamber 3a simultaneously filters out the particles having a large diameter and filters out the particles having a small diameter, so that the sample liquid in the filter chamber 3a satisfies the detection requirement. The filter chamber 3a is in communication with the sample introduction channel 31 to allow the filtered sample solution to flow out and can be added to the detection carrier. The first filter member 41 and the second filter member 42 may be screens or other members that function as a filter.

Preferably, the end of the loading and sealing member 3 away from the tubular body 1 is provided with a spotting nozzle 32, and the loading channel 31 is in communication with the spotting channel of the spotting nozzle 32, and the spotting nozzle 32 is The spotting channel is set to be tapered.

The spotting passage of the spotting nozzle 32 is set to be tapered, that is, the diameter of the passage for the sample to flow out at the outlet of the spotting nozzle 32 is gradually decreased, and the sample amount at which the outlet of the sampling nozzle 32 can flow out at a time is small. It is convenient to accurately control the amount of sample outflow and the position to arrive after the flow is added (or need to be spotted). At the same time, the diameter of the passage for the sample to flow out at the outlet of the spotting nozzle 32 is gradually reduced to further prevent the external environment from affecting the sample in the pipe body 1. Preferably, the spotting nozzle 32 is made of a soft rubber material, and the arrangement can ensure that the spotting nozzle 32 can be sealed by the material deformation of the sample carrier through the deformation of the material, so as to facilitate the extrusion of the sample liquid. Detection in the vector.

Preferably, a cover 33 is also provided, which is detachably coupled to the sample-loading sealing member 3 for shielding the spotting nozzle 32.

In the embodiment of the present invention, a cover 33 is further disposed outside the spotting nozzle 31. The cover 3 is arranged to be detachably connected to the sample-loading sealing member 3, and the connection manner may be a screw connection or an interference fit or other connection manner for shielding the passage for the sample to flow out, so that the entire sampling and loading device forms a closed Space, isolated from the influence of external bacteria, dust and other pollutants, and also prevent the odor of the sample from overflowing and polluting the environment. When it is desired to use the sampling and loading device provided by the present invention, the cover 33 is removed and ready for use.

Preferably, the sampling member 2 is detachably coupled to the loading and sealing member 3.

Preferably, the sampling member 2 is detachably connected to the loading and sealing member 3, and the detachable connection is provided in various manners, for example, by screwing or interference fit, etc., in the present embodiment, the loading and sealing member 3 is disposed. With a connecting cavity, the sampling component 2 can be inserted into the connecting cavity to form an interference fit. The sampling member 2 is detachably coupled to the loading and sealing member 3 for ease of fabrication and assembly. In the present embodiment, the loading sealing member 3 and the tubular body 1 are detachably connected by screwing.

Preferably, the sampling member 2 is any one of a cotton swab, a straw, a sampling brush, a sampling spoon, and a sampling sponge.

a, sampling: sampling using sampling component 2;

b. Dilution: the sampling part 2 to which the sample is attached is placed in the tube body 1 containing the diluent, and the sample sealing member 3 is sealed and connected to the opening of the tube body 1;

c. preparing a sample suspension: shaking the tube 1 to uniformly mix the sample and the diluent to prepare a sample suspension;

d, preparing the application suspension: the tube body 1 is inverted, the sample suspension in the tube is filtered through the first filter member 41, and the particles larger than the pore size of the first filter member 41 remain in the tube body, less than the first a particle suspension of the pore size of the filter member 41 enters the filter chamber 3a, and is filtered by the second filter member 42 to enter a smaller amount of the particle suspension of the second filter member 42 into the sample buffer chamber 3b, thereby making it smaller The first filter member 41 has a pore size, and a particle suspension larger than the pore size of the second filter member 42 remains in the filter chamber 3a;

e. Loading: The deformable tube wall of the tube body 1 is squeezed, so that the suspension in the filter chamber 3a flows out through the sample application channel 31 for loading.

The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments are obvious to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but the scope of the invention is to be accorded

Claims

A sampling and loading device, comprising: a tube body (1), a sampling component (2), a sample loading sealing member (3), and the sample loading sealing member (3) is detachably disposed on the tube body (1) opening and blocking the opening of the pipe body (1) so that the sampling member (2) is housed in the pipe body (1), and the sample-loading sealing member (3) is provided a sample application channel (31) for the sample to flow out, at least part of the tube wall of the tube body (1) is deformable, and the deformable tube wall of the tube body (1) is squeezed to make the sample inside the tube body (1) It flows out through the sample loading channel (31).
The sampling and loading device according to claim 1, characterized in that at least part of the pipe wall of the pipe body (1) is deformable, in particular, at least part of the pipe wall of the pipe body (1) is made of a soft material. to make.
The sampling device according to claim 1, wherein a filter member (4) for filtering a sample is provided in the sample-loading sealing member (3), and the sample in the tube body (1) passes through the sample The filter member (4) enters the sample application channel (31).
The sampling and loading device according to claim 3, wherein the filter member (4) comprises a first filter member (41) and a second filter member (42),

The second filter member (42) is disposed between the sample application channel (31) and the first filter member (41), the first filter member (41) and the second filter member (42) Enclosing a filter chamber (3a), the filter chamber (3a) being in communication with the sample application channel (31);

The second filter member (42) and the tube wall of the sample application channel (31) enclose a sample buffer chamber (3b), and the sample buffer chamber (3b) is in communication with the filter chamber (3a);

The filter aperture of the first filter component (41) is larger than the filter aperture of the second filter component (42), and the sample is filtered by the first filter component (41) and the second filter component (42) and remains in the The sample liquid in the filter chamber (3a) flows out through the sample application channel (31).
The sampling and loading device according to claim 4, wherein a sampling nozzle (32) is provided at one end of the loading and sealing member (3) away from the tubular body (1), and the loading channel (31) And communicating with the spotting channel of the spotting nozzle (32), the spotting channel of the spotting nozzle (32) being set to be tapered.
The sampling and loading device according to claim 5, further comprising a cover (33) detachably coupled to the sample-loading sealing member (3) for shielding The sample mouth (32).
The sample loading device according to any one of claims 1 to 6, characterized in that the sampling member (2) is detachably connected to the sample applying sealing member (3).
The sampling and loading device according to claim 7, wherein the sampling member (2) is any one of a cotton swab, a straw, a sampling brush, a sampling spoon, and a sampling sponge.
A method of using the sample loading device according to any one of claims 4-6, comprising the steps of:

a, sampling: sampling using the sampling component (2);

b. Dilution: the sampling part (2) to which the sample is attached is placed in the tube body (1) containing the diluent, and the sample sealing member (3) is sealedly connected to the opening of the tube body (1);

c. preparing a sample suspension: shaking the tube body (1) to uniformly mix the sample and the diluent to prepare a sample suspension;

d. preparing an application suspension: inverting the tube body (1), the sample suspension in the tube is filtered through the first filter member (41), and the particles larger than the pore size of the first filter member (41) are left in In the tube body, a particle suspension smaller than the pore size of the first filter member (41) enters the filter chamber (3a), and is filtered by the second filter member (42) to be smaller than the particle size of the second filter member (42). Suspending liquid into the sample buffer chamber (3b) such that a particle suspension smaller than the first filter member (41) and larger than the pore size of the second filter member (42) remains in the filter chamber Within (3a);

e. Loading: The deformable tube wall of the tube body (1) is extruded such that the suspension in the filter chamber (3a) flows out through the sample application channel (31) for loading.