JP3275294B2 - Stool inspection kit - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a clinical test sample,
In particular, it is convenient to collect stool used as a sample for detecting occult blood, enzymes, viruses, etc., and transport the quantified amount in a stool lysis buffer in a container in a suspended state. The present invention relates to a stool test kit including a stool container (sample container).

[0002]

2. Description of the Related Art Fecal occult blood tests are widely used for screening for colorectal cancer and rectal cancer. In general, specimens are prepared and inspected in hospitals based on stool collected at home. In qualitative analysis using latex, there is a restriction on the amount of stool at the time of sample preparation, so the designated stool bar is used, but the exact amount of stool is collected using the stool bar In the past, it was difficult to remove excess stool by collecting the stool and then wiping the stool stick with paper or the like.

[0003] However, the stool is dirty and has a strong smell. When the stool is wiped off with paper or the like, the stool may accidentally adhere to the hand or the like. The creator of the specimen often suffered from bad smell, which was a problem. In addition, in the case of a conventional sample container containing a stool lysis buffer, particularly for some subjects such as the elderly, even if the lid was intended to be closed after stool collection, it could not be completely closed, and during transportation, Liquid leakage sometimes occurred.

[0004] To solve the above-mentioned problem, Japanese Unexamined Utility Model Publication No. 2-140468 discloses a stool collection container. This stool collection container is one in which an elongated container main body having a drip portion at the tip is sealed with a lid with a stick, a rubber stopper having a stick insertion hole is fixed inside the container main body, and the lid and the container main body are fixed. The gap is sealed with an O-ring, and a malt and a filter are provided inside the drip part. Insert a stick into the insertion hole of the rubber stopper and wipe off excess stool adhering to the stick. Has become. However, this collection container also requires an expensive rubber stopper, and the rubber stopper must be fixed inside the container body, so that the cost of the collection container increases, and when dropping a stool suspension, Since it is necessary to pierce the sealing part of the drip part with a piercing machine, it is troublesome and there is a risk of soiling the hand, and that the piercing machine needs to be washed each time,
It has disadvantages such as.

In order to improve the disadvantages of the stool container disclosed in Japanese Utility Model Laid-Open Publication No. 2-140468, Japanese Unexamined Patent Publication No.
As disclosed in JP-A-186227, the container includes a container body containing a liquid, a lid provided with a stool collection rod, and a drip portion. By allowing the stool sample to be wiped off, and by piercing the thin end face of the tip of the drip portion, the stool sample dissolved by the liquid is filtered by the filter provided at the lower part of the container body, and is quantitatively dropped. And a container body containing a liquid whose intermediate part is closed with a thin film, a sampling means, and a cap for closing a lower part of the container body as disclosed in JP-A-8-292189. A member is included, so that when the thin film is pierced by the sampling part of the sampling means, an excess stool sample of the sampling means can be wiped off, and the closing part of the nozzle provided on the cap member is removed. And a, have been proposed feces collection containers to be dropped into quantitatively on determination chromatographic specimen filtrate.

However, in any case, these stool collection containers are designed to suspend a stool sample, suspend the stool sample, filter the stool sample through a filter, and drop a liquid obtained by filtering the sample suspension (filtrate) quantitatively. For this reason, a filter is required, and there is a problem in cost. In addition, in the conventional stool collection container, the filtrate is dropped on the filter paper for chromatography, and the first few drops of the filtrate are discarded to improve the reproducibility of the filtrate. In addition to being hung, it was dirty and could be infected with viruses and bacteria.

[0007]

SUMMARY OF THE INVENTION The present invention has been made in view of the above circumstances, and has a lower cost than conventional ones.
An object of the present invention is to provide a stool test kit that is easy to test, has no risk of being infected by viruses or bacteria, and has high test accuracy and reproducibility.

[0008]

SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present invention provides a stool collection rod, a stool lysis buffer contained therein, an opening at the base end and a thin film at the tip end. A sample container having a sample suspension outlet that is closed at step, a sample container provided with a quantitative collection means for quantitatively collecting a stool sample collected with the stool collection rod in the middle part, and a thin film at the sample outlet. A stool inspection kit including a communication needle that can pass through and a determination container containing a filter paper for chromatography therein is adopted. Here, the sample container has a cylindrical container main body having an opening at the base end and a sample suspension outlet closed at the distal end with a thin film, and a closing membrane closing the opening of the container main body at the distal end. And a tubular cap member having an opening at a base end thereof, wherein the stool collection rod can be liquid-tightly inserted into the container body through the closing membrane from the opening of the cap member. A tube having an opening at the base end and a sample suspension outlet closed at the tip end with a thin film may be used so that an excess stool sample is removed when the stool sampling rod penetrates the closing membrane. -Shaped container body, and a through-hole that is provided inside the container body and partitions the inside of the container body, through which a feces sampling rod can be inserted and an excess feces sample can be removed when the feces sampling rod is inserted. A separation wall having the through hole, and the through hole may be closed in a liquid-tight manner by a feces collection rod. In addition, the determination container is a flat and transparent cylindrical container having a wide-mouthed cylindrical sample suspension quantification section at the distal end, and preferably has a communication needle provided in the sample suspension quantification section.

[0009] According to the above configuration of the stool test kit, the communication needle of the determination container is applied to the sample suspension outlet of the sample container, and the communication needle is connected by a simple operation of piercing the thin film with the communication needle. The stool suspension in the sample container can be introduced into the determination container via the sample container. In addition, since the sample container is provided with a quantitative collection means (separation wall having a closed membrane and a perforated hole), excess stool sample collected on the stool collection rod is removed and a fixed amount of stool sample is collected in the sample container. can do. By providing a liquid quantitative section in the determination container, a fixed amount of stool suspension can be introduced into the determination container, so that highly accurate screening can be performed.

[0010]

Next, embodiments of the present invention will be described with reference to the drawings. 1 is a longitudinal sectional view of a stool test kit of the present invention, FIG. 2 is an exploded sectional view of the determination container shown in FIG. 1, and FIG. 3 is a stool test kit shown in FIG. FIG. FIG. 4 is a longitudinal sectional view showing another embodiment of the present invention (a judgment container is omitted). As shown in FIGS. 1 to 4, the stool test kit of the present invention contains a stool collection rod 1 and a stool lysis buffer (omitted) therein, and has an opening at the base end. A quantitative sampling means 21 for quantitatively sampling a stool sample collected with the stool sampling rod 1 is provided at an intermediate portion, which has a liquid outlet (sample suspension outlet) 31 closed with a thin film 32 at the tip. Sample container A1,
A2 and a communication needle 4 capable of penetrating the thin film 32 of the liquid outlet 31
And a determination container B in which a filter paper (filter paper for chromatography) 6 is stored.

As shown in FIGS. 1 and 4, the stool collection rod 1 has a relatively large-diameter grip portion 11 at the base end, which is a portion to be gripped by hand when collecting stool, and sample containers A1 and A2. An engaging portion 12 of an intermediate portion to be engaged and a sample collecting portion 13 at a distal end which is a portion to which a stool sample is attached are formed. On the outer wall of the engaging portion 12, a female screw 2 formed on inner walls of the sample containers A1 and A2 is provided.
A male screw 14 to be screwed with the screw 2 is formed. The sample collection unit 13 has a plurality of grooves (not shown) formed near the tip thereof, and the sample collection units 13 and the quantitative collection means (the closing membrane 21 in FIG. 1 and the separation wall 25 in FIG. 4) for A2. ), The excess stool is scraped off here, so that only the stool sample attached to this groove is collected.

As shown in FIGS. 1 and 4, the sample containers A1 and A2 have a stool dissolution buffer (omitted) therein.
Is housed, has an opening at the base end, and a thin film 3 at the tip end.
2 has a liquid take-out port (sample suspension take-out port) 31 closed at 2 and a quantitative collection means (21, 25) for quantitatively collecting a stool sample collected by the feces collection rod 1 at an intermediate portion. ing. The sample container A1 shown in FIG. 1 has an opening at a base end and a container body 3 having a liquid outlet 31 closed at a distal end with a thin film 32.
And a cylindrical cap member 2 having an opening at the base end and a closing film 21 at the tip end as a quantitative sampling means, and the opening 33 of the container body 3 is provided with a cap inserted therein. It is closed by the closing film 21 of the member 2. A female screw 22 is formed on the inner wall of the cap member 2,
The male thread 14 of the engaging portion 12 of the feces sampling rod 1 can be screwed.

A sample container A2 shown in FIG. 4 has a tubular container body 3 having an opening at the base end and a liquid outlet 31 closed at the distal end with a thin film 32, and is provided inside the container body 3. A partition wall 25 that partitions the inside of the container body 3 and that has a through hole 26 through which the feces sampling rod 1 can be inserted and through which the extra feces sample can be removed when the feces sampling rod 1 is inserted; The through hole 26 can be closed in a liquid-tight manner with the stool collection rod 1. A female screw 22 is formed on the inner wall of the opening of the container body 3 so that the female screw 22 can be screwed with the male screw 14 of the engaging portion 12 of the stool collection rod 1.

As shown in FIGS. 1 and 2, the determination container B includes a communication needle 4 which can pierce the thin film 32 of the liquid outlet 31;
It is composed of a container main body 5 in which a filter paper 6 for chromatography is accommodated, and the container main body 5 has a flat and transparent liquid quantitative section (sample suspension quantitative section) 51 at the tip. It is preferably formed in a cylindrical container and provided with the communication needle 4 protruding from the liquid metering section 51. Here, the communication needle 4
Needle 4 for penetrating the thin film 32 of the sample containers A1 and A2
1 and a cylindrical guide 42 and a skirt portion 43 having an inner diameter slightly larger than the outer diameter of the liquid outlet 31. The opening 53 of the liquid metering portion 51 is formed by the skirt portion 43 which is inserted and fixed thereto. It is closed. Further, the base end side of the determination container B is a flat filter paper accommodating portion 52, in which the filter paper 6 is accommodated. Note that the communication needle 4 may be capped (not shown).

As a material for forming the stool test kit, a transparent synthetic resin such as polyethylene, polypropylene, polyvinyl chloride, polyester, or polystyrene is preferably used for the container body 3 of the sample containers A1 and A2. A flexible resin such as polyethylene, polypropylene, or polyester is preferably used for the second or separation wall 25, and a synthetic resin such as ABS resin, polypropylene, or polyethylene is preferably used for the stool stick 3. For the determination container B, the same synthetic resin as that of the container body 3 can be adopted. As the stool dissolution buffer, a solution containing a trace amount of sodium azide as a preservative, a solution containing ammonium chloride, or the like is used.

In use, for a stool test kit for the sample container A1, a stool sample is attached to the sample collection section 13 of the stool collection rod 1 and then inserted through the opening 34 of the sample container A1. Excess stool attached to the rod 1 is neatly scraped off here when penetrating the closing membrane 21, and the specimen container A
In 1, only a fixed amount of stool sample, that is, only stool attached to the groove of the sample collection unit 13 is collected. Since the stool sample collected in the sample container A1 is naturally dissolved in the stool dissolution buffer to form a suspension, the closure film 32 of the liquid outlet 31 of the sample container A1 is pierced with the communication needle 4 of the determination container B. If passed, judgment container B
The suspension can flow down into the liquid metering section 51. At this time, the amount of the suspension flowing down into the liquid metering section 51 is substantially equal to the volume of the liquid metering section 51 because the filter paper 6 is stored in the filter paper housing section with a slight gap left therebetween. The liquid can be quantitatively collected in the determination container B.

For the stool test kit for the sample container A2, the stool collection rod 1 is removed from the sample container A2, a stool sample is attached to the sample collection section 13, and the stool sample is attached to the sample container A2.
When the feces are inserted through the opening 34, the extra feces adhering to the feces collection rod 1 are neatly scraped off when passing through the through holes 26 of the separation wall 25, and a fixed amount of feces sample, that is, Only the stool attached to the groove of the collection unit 13 is collected. The stool sample collected in the sample container A2 is naturally dissolved in the stool lysis buffer to form a suspension.
When the closing film 32 of the liquid outlet 31 is pierced by the communication needle 4 of the determination container B, the suspension can flow down into the liquid metering section 51 of the determination container B, as in the case of the sample container A1. .

[0018]

As is apparent from the above description, the use of the stool inspection kit of the present invention makes it possible to reduce the cost, reduce the production time, and improve the reproducibility. An inspection tool can be provided. Also,
Adopting a determination container with a communication needle, the operation of only piercing the thin film of the liquid outlet of the sample container with the communication needle can introduce the suspension into the filter paper for chromatography of the determination container, The operation is simple, and there is no fear of soiling hands etc. with the suspension.

[Brief description of the drawings]

FIG. 1 is a longitudinal sectional view of a stool inspection kit of the present invention.

FIG. 2 is an exploded sectional view of the determination container shown in FIG.

FIG. 3 is a view showing a state where a stool collection rod is inserted into a sample container in the stool test kit shown in FIG. 1;

FIG. 4 is a longitudinal sectional view showing another embodiment of the present invention (a determination container is omitted).

[Explanation of symbols]

 A1 A2 Sample container B Judgment container 1 Sampling rod 2 Cap member 21 Closure membrane 25 Separation wall 26 Through hole 3 Container main body 31 Liquid outlet 32 Thin film 4 Communication needle 5 Container main body 51 Liquid metering section 6 Filter paper

Claims (4)

(57) [Claims] 1. A feces collection rod, a feces lysis buffer contained therein, an opening at a base end, and a sample suspension outlet at a tip end closed with a thin film. A sample container provided with a quantitative collection means for quantitatively collecting a stool sample collected by the stool collection rod, and a communication needle capable of piercing a thin film of the sample suspension outlet, for chromatography A stool inspection kit, comprising: a determination container containing filter paper. 2. A sample container having a cylindrical container body having an opening at a base end and a sample suspension outlet closed at a tip portion with a thin film, and an opening of the container body at a tip portion. A tubular cap member having a closing membrane and having an opening at a base end, wherein the stool collection rod can be inserted in a liquid-tight manner through the closing membrane from the opening of the cap member through the closing membrane. The stool test kit according to claim 1, wherein an excess stool sample is removed when the stool collection rod penetrates the closing membrane. 3. A sample container having a cylindrical container body having an opening at a base end and a sample suspension outlet closed at a tip portion with a thin film, and an inside of the container body provided inside the container body. And a separation wall having a through hole through which a feces sampling rod can be inserted and an extra feces sample can be removed when the feces sampling rod is inserted, wherein the through hole is a feces sampling rod. 2. The stool test kit according to claim 1, which is liquid-tightly closed. 4. The determination container is a flat and transparent cylindrical container having a wide-mouthed cylindrical sample suspension quantification section at a distal end thereof,
2. A communication needle is provided in the sample suspension quantification unit.
A stool test kit according to any one of claims 1 to 3.