CN216449266U - Automatic detection device integrating bioaerosol sampling and nucleic acid detection - Google Patents
Automatic detection device integrating bioaerosol sampling and nucleic acid detection Download PDFInfo
- Publication number
- CN216449266U CN216449266U CN202121579586.3U CN202121579586U CN216449266U CN 216449266 U CN216449266 U CN 216449266U CN 202121579586 U CN202121579586 U CN 202121579586U CN 216449266 U CN216449266 U CN 216449266U
- Authority
- CN
- China
- Prior art keywords
- chip
- nucleic acid
- sampling
- detected
- unit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The utility model discloses an automatic detection device integrating bioaerosol sampling and nucleic acid detection, wherein a sampling and sample feeding unit and a nucleic acid detection unit of the automatic detection device are both arranged at the top of a chip conveying unit; the sampling and sample feeding unit is used for filling sampling liquid into the chip; the chip conveying unit is used for conveying the chip filled with the sampling liquid to the nucleic acid detection unit for detection and moving the detected chip away from the nucleic acid detection unit; the nucleic acid detection unit is used for detecting the sampling liquid filled in the chip; the automatic control unit controls the sampling and sample feeding unit, the chip conveying unit and the nucleic acid detection unit to act, and obtains the detection result of the nucleic acid detection unit. Above-mentioned automatic detection device is with biological aerosol sampling technique and nucleic acid detection technology integration an organic whole, can improve biological aerosol detection efficiency, reduces the manual work, does not need the laboratory of specialty also can operate, is applicable to most scenes.
Description
Technical Field
The utility model relates to the technical field of bioaerosol monitoring, in particular to an automatic detection device integrating bioaerosol sampling and nucleic acid detection.
Background
In recent years, SARS (atypical pneumonia) virus, ebola, influenza and new crown epidemic are widely spread in the global scope, and the life health of human beings is greatly harmed. The aerosol is an important way for spreading viruses and bacteria, and can provide scientific basis and effective technical support for epidemic situation prevention and control in the detection of the bioaerosol.
The traditional bioaerosol detection method is to collect bioaerosol into media such as culture medium, liquid, filter membrane and the like through a bioaerosol sampler, and then detect the collected sample through schemes such as a culture method, an immunological technique, a nucleic acid technique and the like, so as to judge whether certain microorganisms exist in the ambient air. The method has the advantages that the types and the concentrations of microorganisms can be monitored, and the defects that the sampling and detection operation is complex, the operation of professional personnel and a professional laboratory are needed, the time consumption is long, and the timeliness is poor. Therefore, the existing bioaerosol detection has the problems of low sampling, detection and separation, low automation degree and the like.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides an automatic detection device integrating bioaerosol sampling and nucleic acid detection, which uses a nucleic acid detection technology for bioaerosol detection and integrates the bioaerosol sampling technology and the nucleic acid detection technology into a whole, so that the bioaerosol detection efficiency can be improved, the labor can be reduced, the device can be operated in a laboratory without professional requirements, and the device is suitable for most scenes.
The utility model adopts the following specific technical scheme:
the automatic detection device integrates biological aerosol sampling and nucleic acid detection, and comprises a sampling and sample feeding unit, a chip conveying unit, a nucleic acid detection unit and an automatic control unit;
the sampling and sample feeding unit and the nucleic acid detection unit are both arranged at the top of the chip conveying unit; the sampling and sample feeding unit is used for filling sampling liquid into the chip; the chip conveying unit is used for conveying the chip filled with the sampling liquid to the nucleic acid detection unit for detection and moving the detected chip away from the nucleic acid detection unit; the nucleic acid detection unit is used for detecting the sampling liquid filled in the chip;
the automatic control unit controls the sampling and sample feeding unit, the chip conveying unit and the nucleic acid detection unit to act, and obtains the detection result of the nucleic acid detection unit.
Furthermore, the nucleic acid detection unit is provided with a chip inlet and a chip outlet and comprises a conveying module, a nucleic acid extraction module, a nucleic acid amplification module and a fluorescence detection module;
the conveying module conveys detection kits among the nucleic acid extraction module, the nucleic acid amplification module and the fluorescence detection module, and is used for conveying sample liquid required in the processes of nucleic acid extraction, amplification and detection.
Furthermore, the chip conveying unit comprises a chip in-out module and a chip conveying module positioned at the bottom of the chip in-out module;
the chip in-out module is used for pushing a chip to be detected to enter the nucleic acid detection unit through the chip inlet and outlet and transporting the detected chip in the nucleic acid detection unit to the chip conveying module;
the chip transmission module is used for conveying chips.
Furthermore, the chip conveying module is a chain type conveying mechanism and comprises a stepping motor, a transmission chain in transmission connection with the stepping motor and a chain straight lug fixedly arranged on the transmission chain;
the chain straight lug is used for pushing the chip to move.
Furthermore, a chip slot to be detected for storing a chip to be detected is arranged on one side of the chip transmission module, and a detected chip slot for storing a detected chip is arranged on the other side of the chip transmission module; chip frames for rapidly picking and placing chips are arranged in the chip clamping groove to be detected and the chip clamping groove to be detected;
the nucleic acid detection unit is positioned between the chip card slot to be detected and the chip card slot to be detected;
an electric push rod is arranged at the bottom of the inspected chip clamping groove and used for pushing the inspected chip into the inspected chip clamping groove and stacking the inspected chip;
and a pawl for restraining the inspected chip from falling is arranged in the inspected chip clamping groove.
Furthermore, a horizontal electromagnetic baffle is arranged between the nucleic acid detection unit and the card slot of the chip to be detected;
the electromagnetic baffle is used for fixing the front and back directions of the chip to be detected and preventing the chip to be detected from being inclined in the conveying process.
Furthermore, a transmission chain zero point sensor and a transmission chain positioning sensor are arranged below the upper ring of the transmission chain, and the transmission chain zero point sensor and the positioning sensor are used for detecting the position of the to-be-detected chip.
Furthermore, the chip in-out module comprises a chip in-out sliding table, a sucker limit sensor, a sucker zero position sensor, a mounting plate, a sucker air pump and a negative pressure switch;
the chip in-out sliding table can move in the front-back direction and is arranged on one side of the nucleic acid detection unit, the front end of the chip in-out sliding table is provided with a sucker limiting sensor for detecting the insertion depth of a chip to be detected, and the rear end of the chip in-out sliding table is provided with a sucker zero position sensor for determining the zero position of the chip in-out sliding table;
the sucking disc is fixedly arranged on the chip in-out sliding table through the mounting plate;
the sucker is connected with the sucker air pump through a silicone tube, and a negative pressure switch is connected in the silicone tube.
Furthermore, the sampling and sample-feeding unit comprises a liquid storage bottle, a liquid supplementing pump, a sampling bottle, a sampling head, an air pump, a liquid outlet pump, a sliding table, a mechanical positioning clamp and a transfusion needle;
the sliding table can move in the vertical direction and is arranged above the transmission chain, and the mechanical positioning clamp and the infusion needle are fixedly arranged on a sliding block of the sliding table;
the mechanical positioning clamp is used for positioning the chip;
the transfusion needle is used for puncturing the chip, filling sampling liquid and discharging waste liquid to the waste liquid bottle;
the liquid storage bottle, the liquid supplementing pump and the liquid supplementing port of the sampling bottle are connected sequentially through a silicone tube;
the air pump is connected with an air outlet of the sampling head and is used for enabling the interior of the sampling bottle to form negative pressure and aerosol particles to enter the sampling bottle for sampling;
the liquid outlet of the sampling bottle, the liquid outlet pump and the infusion needle are sequentially connected through a silicone tube;
the axis of the infusion needle coincides with the axis of a silica gel plug of the chip to be detected, which is positioned at the position for filling the sampling liquid.
Furthermore, the sampling and sample-sending unit further comprises a waste liquid bottle, an infusion needle zero position sensor and an infusion needle limit sensor;
the waste liquid bottle is positioned below the infusion needle and used for storing the residual sampling liquid in the sampling bottle;
when the infusion needle is in the maximum displacement, the infusion needle is positioned in the bottle opening of the waste liquid bottle;
the infusion needle zero position sensor is positioned at the top of the sliding table and used for determining the zero position of the infusion needle;
the infusion needle limiting sensor is installed at the bottom of the sliding table and used for limiting the infusion needle.
Has the advantages that:
the automatic detection device disclosed by the utility model has the advantages that the nucleic acid detection technology is used for detecting the bioaerosol, the bioaerosol sampling technology and the nucleic acid detection technology are integrated, the bioaerosol detection efficiency can be improved, the manpower is reduced, the device can be operated without needing a professional laboratory, the device is suitable for most scenes, the aerosol propagation rule can be researched and explored in daily monitoring of the bioaerosol, the propagation of pathogenic microorganisms is early warned, and the technical support is provided for epidemic prevention and control.
Drawings
FIG. 1 is a schematic structural diagram of an automated inspection apparatus according to the present invention;
FIG. 2 is a schematic diagram showing the schematic structure of a nucleic acid detecting unit of the automated detecting apparatus according to the present invention;
FIG. 3 is a schematic structural diagram of a sampling and sample-feeding unit of the automatic detection device of the present invention;
FIG. 4 is a schematic structural diagram of a chip transfer module of the automatic detection apparatus according to the present invention;
FIG. 5 is a schematic diagram of a chip in-out module of the automatic detection apparatus according to the present invention;
wherein, 1-a sampling and sample feeding unit, 2-a chip conveying unit, 3-a nucleic acid detecting unit, 4-an automatic control unit, 5-a chip in-and-out module, 6-a chip conveying module, 7-a detected chip clamping groove, 8-a chip clamping groove to be detected, 9-a chip to be detected, 10-a liquid storage bottle, 11-a liquid supplementing pump, 12-a sampling bottle, 13-an air pump, 14-a liquid outlet pump, 15-a sliding table, 16-a mechanical positioning clamp, 17-an infusion needle, 18-a transmission chain, 19-a waste liquid bottle, 20-an infusion needle zero position sensor, 21-an infusion needle limit sensor, 22-a chip frame, 23-a chain straight lug, 24-a transmission chain zero position sensor, 25-a transmission chain positioning sensor and 26-a stepping motor, 27-electric push rod, 28-pawl, 29-detected chip, 30-electromagnetic baffle, 31-chip in-and-out sliding table, 32-suction cup limit sensor, 33-suction cup zero position sensor, 34-mounting plate, 35-suction cup, 36-suction cup air pump, 37-negative pressure switch, 41-conveying module, 42-nucleic acid extraction module, 43-nucleic acid amplification module and 44-fluorescence detection module
Detailed Description
The utility model is described in detail below by way of example with reference to the accompanying drawings.
Referring to fig. 1, the present invention provides an automatic detection device integrating bioaerosol sampling and nucleic acid detection, which comprises a sampling and sample feeding unit 1, a chip conveying unit 2, a nucleic acid detection unit 3 and an automatic control unit 4; the sampling and sample feeding unit 1 and the nucleic acid detection unit 3 are both arranged at the top of the chip conveying unit 2; the sampling and sample-sending unit 1 is used for filling sampling liquid into the chip; the chip conveying unit 2 is used for conveying the chip filled with the sampling liquid to the nucleic acid detection unit 3 for detection, and moving the detected chip away from the nucleic acid detection unit 3; the nucleic acid detection unit 3 is used for detecting the sampling liquid filled in the chip; the automatic control unit 4 controls the sampling and sample-feeding unit 1, the chip-transporting unit 2, and the nucleic acid-detecting unit 3 to operate, and obtains the detection result of the nucleic acid-detecting unit 3. The chip may be a microfluidic chip.
The automatic detection device comprises a sampling and sample-feeding unit 1 for sampling and filling sampling liquid into a chip, a chip conveying unit 2 for conveying the chip, a nucleic acid detection unit 3 for detecting the sampling liquid in the chip and an automatic control unit 4 for controlling each unit to work according to a preset program, wherein the nucleic acid detection technology is used for detecting bioaerosol through the sampling and sample-feeding unit 1, the chip conveying unit 2 and the nucleic acid detection unit 3, the bioaerosol sampling technology and the nucleic acid detection technology are integrated into a whole, so that the bioaerosol sampling and nucleic acid detection are integrated and automated, the aerosol detection is realized, the bioaerosol detection efficiency can be improved, the manpower is reduced, a professional laboratory is not required, and the automatic detection device is suitable for most scenes; the real-time uploading of the bioaerosol detection information is realized through the automatic control unit 4, the aerosol propagation rule can be researched and explored in the daily monitoring of the bioaerosol, the propagation of pathogenic microorganisms is early warned, and the technical support is provided for epidemic prevention and control.
In a specific embodiment, as shown in FIG. 2, the nucleic acid detecting unit 3 is provided with a chip inlet and outlet through which the chip is fed into the nucleic acid detecting unit 3, and includes a conveying module 41, a nucleic acid extracting module 42, a nucleic acid amplifying module 43, and a fluorescence detecting module 44; the delivery module 41 delivers the detection kit among the nucleic acid extraction module 42, the nucleic acid amplification module 43, and the fluorescence detection module 44 for delivering the sample liquid required in the nucleic acid extraction, amplification, and detection processes.
The nucleic acid detection unit 3 can integrate the procedures of sample pretreatment, nucleic acid amplification, detection and the like into one microfluidic chip to complete, and the sample pretreatment, nucleic acid amplification and detection are integrated and automated through a nucleic acid detection integrated system; the sample liquid is detected in the nucleic acid detection chip, and a sample reaction pool in the chip is sealed with the micro-flow channel and the outside, so that the pollution of nucleic acid to the environment is avoided; the nucleic acid detection chip is designed in an integrated full-automatic way without manual intervention.
Further, the chip conveying unit 2 includes a chip in-out module 5 and a chip transfer module 6 located at the bottom of the chip in-out module 5; the chip in-out module 5 is used for pushing the chip 9 to be detected to enter the nucleic acid detection unit 3 through the chip inlet and outlet, and transporting the detected chip 29 in the nucleic acid detection unit 3 to the chip transmission module 6; the chip transfer module 6 is used for conveying chips. As shown in fig. 4, the chip transfer module 6 may be a chain type conveying mechanism, and includes a step motor 26, a transmission chain 18 in transmission connection with the step motor 26, and a chain straight lug 23 fixedly mounted on the transmission chain 18; the straight chain lug 23 is used for pushing the chip to move. A chip slot 8 to be detected for storing a chip 9 to be detected is arranged on one side of the chip conveying module 6, a detected chip slot 7 for storing a detected chip 29 is arranged on the other side of the chip conveying module, and the lower surface of the chip slot 8 to be detected and the lower surface of the detected chip slot 7 are both superposed with the lower surfaces of the chip inlet and the chip outlet of the nucleic acid detecting unit 3; chip frames 22 for rapidly picking and placing chips are arranged in the chip clamping grooves 8 to be detected and the detected chip clamping grooves 7, and the chip frames 22 are convenient for taking out the detected chips 29 from the detected chip clamping grooves 7 at one time or putting a plurality of chips 9 to be detected into the chip clamping grooves 8 to be detected at one time; the nucleic acid detection unit 3 is positioned between the chip card slot 8 to be detected and the chip card slot 7 to be detected; an electric push rod 27 is arranged at the bottom of the inspected chip card slot 7, and the electric push rod 27 is used for pushing the inspected chip 29 into the inspected chip card slot 7 to stack the inspected chip 29; a pawl 28 for restraining the inspected chip 29 from falling is arranged in the inspected chip slot 7, the inspected chip 29 can be restrained in the inspected chip slot 7 through the pawl 28, and the inspected chip 29 can be stacked and processed by continuously pushing the inspected chip 29 up and down through the electric push rod 27.
As shown in FIGS. 4 and 5, a horizontal electromagnetic shield 30 is provided between the nucleic acid detecting unit 3 and the card slot 8 of the chip to be detected; the electromagnetic baffle 30 is used for fixing the front and back directions of the chip 9 to be detected and preventing the chip 9 to be detected from being inclined in the conveying process. And a transmission chain zero sensor 24 and a transmission chain positioning sensor 25 are arranged below the upper ring of the transmission chain 18, and the transmission chain zero sensor 24 and the transmission chain positioning sensor 25 are used for detecting the position of the chip 9 to be detected.
The conveying process of the chip conveying unit 2 is as follows: the initial state is that the transmission chain zero sensor 24 is triggered, the chain straight lug 23 is positioned at the right side of the chip 9 to be detected, after the chip conveying process is started, the stepping motor 26 is electrified to rotate and drives the transmission chain 18 to run anticlockwise, the chip 9 to be detected can directly fall on the transmission chain 18 through gravity, the chain straight lug 23 pushes the chip 9 to be detected to move leftwards, the chip 9 to be detected is prevented from skewing in the conveying process through the electromagnetic baffle 30, after the transmission chain positioning sensor 25 is triggered, the stepping motor 26 stops rotating, the chip 9 to be detected is aligned with the chip inlet and outlet of the nucleic acid detecting unit 3, after the sampling sample conveying-electromagnetic baffle 30 attracting-card feeding-electromagnetic baffle 30 disconnecting-detecting-card withdrawing process is finished, the stepping motor 26 is started, the chain straight lug 23 pushes the detected chip 29 to move leftwards, and the detected chip 29 is conveyed to the position below the detected chip clamping groove 7, the electric push rod 27 moves upward to push the inspected chip 29 into the inspected chip slot 7 for stacking, and the pawl 28 is matched to limit the inspected chip 29 in the inspected chip slot 7.
Specifically, as shown in fig. 5, the chip in-out module 5 includes a chip in-out sliding table 31, a suction cup limit sensor 32, a suction cup zero position sensor 33, a mounting plate 34, a suction cup 35, a suction cup air pump 36, and a negative pressure switch 37; the chip in-out sliding table 31 can move in the front-back direction and is installed on the left side of the nucleic acid detection unit 3, a suction cup limit sensor 32 for detecting the insertion depth of the chip 9 to be detected is arranged at the front end of the chip in-out sliding table 31, and the suction cup limit sensor 32 is used for detecting the insertion depth of the chip 9 to be detected; a suction cup zero position sensor 33 for determining the zero position of the chip in-out sliding table 31 is arranged at the rear end of the chip in-out sliding table 31, and the suction cup zero position sensor 33 is used for determining the zero position of the chip in-out sliding table 31; when the chip in-out sliding table 31 is at the maximum displacement, the detection positions of the chip 9 to be detected and the nucleic acid detection unit 3 are superposed; the sucking disc 35 is fixedly arranged on the chip in-out sliding table 31 through the mounting plate 34, the horizontal central line of the sucking disc 35 is flush with the horizontal central line of the chip 9 to be detected, and the sucking disc is used for pushing the chip 9 to be detected into the nucleic acid detection unit 3 and pulling the detected chip 29 out of the nucleic acid detection unit 3; the suction cup 35 is connected with the suction cup air pump 36 through a silicone tube, a negative pressure switch 37 is connected in the silicone tube, and the negative pressure switch 37 is triggered after the suction cup 35 sucks the chip.
The working process of the chip in-out module 5 is as follows: the initial state is that the suction cup zero position sensor 33 is triggered, the chip 9 to be detected finishes the filling of the sampling liquid, the sliding table 15 for filling the sampling liquid is zero-returned, after the chip in-out program is started, the electromagnetic baffle 30 is electrified and sucked, the chip in-out sliding table 31 is started and drives the mounting plate 34 and the suction cup 35 to push the chip 9 to be detected forward, the chip 9 to be detected is inserted into the nucleic acid detection unit 3, after the suction cup limit sensor 32 is triggered, the electromagnetic baffle 30 is powered off, the chip in-out sliding table 31 runs for a certain distance in a reverse direction, after the detection of the nucleic acid detection unit 3 is finished, the nucleic acid detection unit 3 partially withdraws the detected chip 29, after the card withdrawing signal is received, the suction cup air pump 36 is started, the chip in-out sliding table 31 runs in the forward direction, after the suction cup 35 is attached to the detected chip 29, the negative pressure switch 37 is triggered, the chip in-out sliding table 31 drives the detected chip 29 to run in the reverse direction through the suction cup 35, and the suction cup zero position sensor 33 is stopped after being triggered, and finishing the chip in and out flow.
As shown in fig. 3, the sampling and sample-feeding unit 1 includes a liquid storage bottle 10, a fluid infusion pump 11, a sampling bottle 12, a sampling head, an air pump 13, a fluid outlet pump 14, a sliding table 15, a mechanical positioning clamp 16 and an infusion needle 17; the sliding table 15 can move in the vertical direction and is arranged above the transmission chain 18, and the mechanical positioning clamp 16 and the infusion needle 17 are fixedly arranged on a sliding block of the sliding table 15; the mechanical positioning clamp 16 is used for aligning and positioning the chip; the transfusion needle 17 is used for puncturing the chip and filling the sampling liquid, and discharging the redundant sampling liquid into a waste liquid bottle 19 after the sampling liquid is filled into the chip; the liquid storage bottle 10, the liquid supplementing pump 11 and the liquid supplementing opening of the sampling bottle 12 are connected through a silicone tube in sequence; the sampling head is covered at the opening of the sampling bottle 12 and is provided with an air inlet and an air outlet which are communicated with the sampling bottle 12, the air inlet is communicated with the atmosphere, the air outlet is connected with an air pump 13, the air pump 13 provides power for sampling and is used for enabling the interior of the sampling bottle 12 to form negative pressure, and aerosol particles enter the sampling bottle 12 for sampling; a liquid outlet of the sampling bottle 12, the liquid outlet pump 14 and the transfusion needle 17 are connected in sequence through a silicone tube; the axis of the infusion needle 17 coincides with the axis of a silica gel plug of the chip 9 to be detected positioned at the position for filling the sampling liquid. The sampling and sample-sending unit 1 can also comprise a waste liquid bottle 19, an infusion needle zero-position sensor 20 and an infusion needle limit sensor 21; waste liquid bottle 19 is located below infusion needle 17 for storing the remaining sample liquid in sample bottle 12, such as: sampling fluid other than input to the chip; when the transfusion needle 17 is at the maximum displacement, the transfusion needle 17 is positioned in the bottle mouth of the waste liquid bottle 19; an infusion needle zero position sensor 20 is positioned at the top of the sliding table 15 and used for determining the zero position of the infusion needle 17; infusion needle limit sensor 21 is installed in the bottom of slip table 15 for carry on spacingly to infusion needle 17.
When the sampling and sample-feeding unit 1 works, the liquid supplementing pump 11 quantitatively inputs the collected liquid in the liquid storage bottle 10 into the sampling bottle 12, the air pump 13 is started to sample, after sampling is completed, the sliding table 15 moves downwards, the mechanical positioning clamp 16 completes the centering and positioning of the chip in the stroke that the infusion needle 17 is not contacted with the chip 9 to be detected, then the infusion needle 17 is inserted into the chip 9 to be detected, the liquid outlet pump 14 is used for quantitatively adding the sampled liquid into the chip 9 to be detected, the sliding table 15 returns to the zero point after the filling is completed, and the residual sampled liquid is added into the waste liquid bottle 19 by the liquid outlet pump 14 in the chip detection stage.
The working process of the automatic detection device is as follows: when the liquid storage bottle 10 and the chip 9 to be detected are installed in place, the operation is started, the transmission chain 18, the sucking disc 35 and the infusion needle 17 return to zero, the fluid infusion pump 11 is started, quantitative liquid is extracted from the liquid storage bottle 10 through a silicone tube to the sampling bottle 12, the sampling time can be set through the automatic control unit 4, after the sampling is finished, the chip conveying unit 2 conveys the chip 9 to be detected to the outer side of the chip inlet and outlet of the nucleic acid detection unit 3 through the straight lug 23 of the chain, the sliding table 15 of the sampling and sample conveying unit 1 drives the mechanical positioning clamp 16 and the infusion needle 17 to move downwards, in the process that the infusion needle 17 does not contact the chip, the mechanical positioning clamp 16 aligns the chip to enable the chip to be opposite to the chip inlet of the nucleic acid detection unit 3, after the chip is aligned, the head of the infusion needle 17 is inserted into the silicone plug of the chip, the quantitative sampling fluid is input, after the conveying is finished, the sliding table 15 drives the mechanical positioning clamp 16 and the infusion needle 17 to return to the chip, the chip is pushed into the nucleic acid detection unit 3 by the chip in-out module 5 for detection, after detection is finished, the nucleic acid detection unit 3 partially withdraws the detected chip 29, the chip in-out module 5 conveys the detected chip 29 to the position for filling the sampling liquid again through the suction cup 35, the pipeline of the suction cup 35 is provided with a negative pressure switch 37, and the motion state of the detected chip 29 in the process is judged; after the inspected chip 29 reaches the filling and sampling liquid level, the chip conveying unit 2 operates to convey the inspected chip 29 to the position below the inspected chip clamping groove 7, and after the inspected chip 29 is in place, the electric push rod 27 is started to push the inspected chip 29 into the inspected chip clamping groove 7, so that the inspected chip 29 is limited in the inspected chip clamping groove 7 by utilizing the unidirectional property of the pawl 28, and the one-time detection process is finished.
The detection result of the nucleic acid detection unit 3 can be transmitted to the automatic control unit 4, and the automatic control unit 4 can transmit the nucleic acid detection information to a server for storage through a wired network or a wireless network; and the user remotely checks the bioaerosol monitoring information and controls the monitoring through a mobile phone or a computer terminal.
In summary, the above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The automatic detection device integrating bioaerosol sampling and nucleic acid detection is characterized by comprising a sampling and sample feeding unit, a chip conveying unit, a nucleic acid detection unit and an automatic control unit;
the sampling and sample feeding unit and the nucleic acid detection unit are both arranged at the top of the chip conveying unit; the sampling and sample feeding unit is used for filling sampling liquid into the chip; the chip conveying unit is used for conveying the chip filled with the sampling liquid to the nucleic acid detection unit for detection and moving the detected chip away from the nucleic acid detection unit; the nucleic acid detection unit is used for detecting the sampling liquid filled in the chip;
the automatic control unit controls the sampling and sample feeding unit, the chip conveying unit and the nucleic acid detection unit to act, and obtains the detection result of the nucleic acid detection unit.
2. The automated detection apparatus of claim 1, wherein the nucleic acid detection unit is provided with a chip inlet and outlet, and comprises a delivery module, a nucleic acid extraction module, a nucleic acid amplification module, and a fluorescence detection module;
the conveying module conveys detection kits among the nucleic acid extraction module, the nucleic acid amplification module and the fluorescence detection module, and is used for conveying sample liquid required in the processes of nucleic acid extraction, amplification and detection.
3. The automated inspection device of claim 2, wherein the chip transport unit comprises a chip in-out module and a chip transfer module at a bottom of the chip in-out module;
the chip in-out module is used for pushing a chip to be detected to enter the nucleic acid detection unit through the chip inlet and outlet and transporting the detected chip in the nucleic acid detection unit to the chip conveying module;
the chip transmission module is used for conveying chips.
4. The automatic detection device according to claim 3, wherein the chip transfer module is a chain type conveying mechanism, and comprises a stepping motor, a transmission chain in transmission connection with the stepping motor, and a chain straight lug fixedly arranged on the transmission chain;
the chain straight lug is used for pushing the chip to move.
5. The automatic detection device according to claim 4, wherein a chip slot to be detected for storing a chip to be detected is provided at one side of the chip transfer module, and a detected chip slot for storing a detected chip is provided at the other side; chip frames for rapidly picking and placing chips are arranged in the chip clamping groove to be detected and the chip clamping groove to be detected;
the nucleic acid detection unit is positioned between the chip card slot to be detected and the chip card slot to be detected;
an electric push rod is arranged at the bottom of the inspected chip clamping groove and used for pushing the inspected chip into the inspected chip clamping groove and stacking the inspected chip;
and a pawl for restraining the inspected chip from falling is arranged in the inspected chip clamping groove.
6. The automated detecting apparatus according to claim 5, wherein a horizontal electromagnetic shield is provided between the nucleic acid detecting unit and the card slot of the chip to be detected;
the electromagnetic baffle is used for fixing the front and back directions of the chip to be detected and preventing the chip to be detected from being inclined in the conveying process.
7. The automatic detection device according to claim 6, wherein a transmission chain zero point sensor and a transmission chain positioning sensor are arranged below the upper ring of the transmission chain, and the transmission chain zero point sensor and the positioning sensor are used for detecting the position of the chip to be detected.
8. The automated inspection device of claim 7, wherein the chip in-out module comprises a chip in-out slide, a suction cup limit sensor, a suction cup zero position sensor, a mounting plate, a suction cup air pump, and a negative pressure switch;
the chip in-out sliding table can move in the front-back direction and is arranged on one side of the nucleic acid detection unit, the front end of the chip in-out sliding table is provided with a sucker limiting sensor for detecting the insertion depth of a chip to be detected, and the rear end of the chip in-out sliding table is provided with a sucker zero position sensor for determining the zero position of the chip in-out sliding table;
the sucking disc is fixedly arranged on the chip in-out sliding table through the mounting plate;
the sucker is connected with the sucker air pump through a silicone tube, and a negative pressure switch is connected in the silicone tube.
9. The automatic detection device according to claim 8, wherein the sampling and sample-sending unit comprises a liquid storage bottle, a waste liquid bottle, a liquid supplementing pump, a sampling bottle, a sampling head, an air pump, a liquid outlet pump, a sliding table, a mechanical positioning clamp and an infusion needle;
the sliding table can move in the vertical direction and is arranged above the transmission chain, and the mechanical positioning clamp and the infusion needle are fixedly arranged on a sliding block of the sliding table;
the mechanical positioning clamp is used for positioning the chip;
the transfusion needle is used for puncturing the chip, filling sampling liquid and discharging waste liquid to the waste liquid bottle;
the liquid storage bottle, the liquid supplementing pump and the liquid supplementing port of the sampling bottle are connected sequentially through a silicone tube;
the air pump is connected with an air outlet of the sampling head and is used for enabling the interior of the sampling bottle to form negative pressure and aerosol particles to enter the sampling bottle for sampling;
the liquid outlet of the sampling bottle, the liquid outlet pump and the infusion needle are sequentially connected through a silicone tube;
the axis of the infusion needle coincides with the axis of a silica gel plug of the chip to be detected, which is positioned at the position for filling the sampling liquid.
10. The automated inspection device of claim 9, wherein the sample presentation unit further comprises an infusate needle zero position sensor and an infusate needle limit sensor;
the waste liquid bottle is positioned below the infusion needle and used for storing the residual sampling liquid in the sampling bottle;
when the infusion needle is in the maximum displacement, the infusion needle is positioned in the bottle opening of the waste liquid bottle;
the infusion needle zero position sensor is positioned at the top of the sliding table and used for determining the zero position of the infusion needle;
the infusion needle limiting sensor is installed at the bottom of the sliding table and used for limiting the infusion needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121579586.3U CN216449266U (en) | 2021-07-13 | 2021-07-13 | Automatic detection device integrating bioaerosol sampling and nucleic acid detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121579586.3U CN216449266U (en) | 2021-07-13 | 2021-07-13 | Automatic detection device integrating bioaerosol sampling and nucleic acid detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216449266U true CN216449266U (en) | 2022-05-06 |
Family
ID=81347535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121579586.3U Active CN216449266U (en) | 2021-07-13 | 2021-07-13 | Automatic detection device integrating bioaerosol sampling and nucleic acid detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216449266U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114774264A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
-
2021
- 2021-07-13 CN CN202121579586.3U patent/CN216449266U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114774264A (en) * | 2022-06-22 | 2022-07-22 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
| CN114774264B (en) * | 2022-06-22 | 2022-09-09 | 至美时代生物智能科技(北京)有限公司 | Automatic air microorganism detection system |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113418757A (en) | Automatic detection device integrating bioaerosol sampling and nucleic acid detection | |
| CN106434318A (en) | Gene detection device adopting biotechnology | |
| CN216449266U (en) | Automatic detection device integrating bioaerosol sampling and nucleic acid detection | |
| CN202230093U (en) | Leucorrhea analyzer | |
| CN214471934U (en) | Automatic sampling device of biological aerosol | |
| CN102745636A (en) | Facility for automatically collecting waste liquid in volumetric flasks | |
| CN111044739A (en) | Sample introduction method and sample introduction system for sample reagent of microfluidic chip | |
| CN112920948A (en) | Nucleic acid detection pretreatment instrument | |
| CN114774264A (en) | Automatic air microorganism detection system | |
| CN206362604U (en) | A kind of micro-biological samples automatic smear device | |
| CN114774265A (en) | Automatic air microorganism detection system | |
| CN216688084U (en) | Nucleic acid extraction detection device | |
| CN113667597B (en) | Sampling gun and method integrating liquid collecting, drip sampling, sample processing and detection | |
| CN215856129U (en) | Collection liquid drips sampling, sample processing, detects sampling rifle of integration | |
| WO2024021222A1 (en) | Exosome extractor | |
| CN114480597B (en) | Digital PCR-based droplet detection method and device | |
| CN216237044U (en) | Automatic change liquid-transfering nucleic acid and draw PCR all-in-one | |
| CN108315243B (en) | Automatic sample adding system | |
| JP7324375B2 (en) | FULLY AUTOMATED GENETIC ANALYSIS EQUIPMENT AND GENETIC ANALYSIS METHOD | |
| CN109596528A (en) | A kind of automatic coagulation analyzer and its analysis method | |
| CN108330097A (en) | A kind of cell culture, detection method | |
| CN209024559U (en) | A kind of 48 hole deep-well plates extracted for full-automatic DNA | |
| CN202994806U (en) | Multi-sample sequential loading device for micro-fluidic chip | |
| CN206244791U (en) | A kind of biotechnology gene assaying device | |
| CN211070130U (en) | Automatic liquid sucking and discharging device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |