CN106967585B - Integrated device for nucleic acid extraction and detection - Google Patents
Integrated device for nucleic acid extraction and detection Download PDFInfo
- Publication number
- CN106967585B CN106967585B CN201710366498.7A CN201710366498A CN106967585B CN 106967585 B CN106967585 B CN 106967585B CN 201710366498 A CN201710366498 A CN 201710366498A CN 106967585 B CN106967585 B CN 106967585B
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- unit
- detection
- nucleic acid
- porous plate
- detection unit
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- 238000001514 detection method Methods 0.000 title claims abstract description 51
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 18
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 18
- 238000000605 extraction Methods 0.000 title claims abstract description 7
- 239000000523 sample Substances 0.000 claims abstract description 35
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 230000003993 interaction Effects 0.000 claims abstract description 5
- 239000012521 purified sample Substances 0.000 claims abstract description 5
- 238000007781 pre-processing Methods 0.000 claims abstract description 4
- 238000005192 partition Methods 0.000 claims description 10
- 244000005700 microbiome Species 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000001821 nucleic acid purification Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
Abstract
The invention belongs to the technical field of a method for measuring or detecting microorganisms, and particularly relates to integrated equipment for extracting and detecting nucleic acid. The integrated equipment for extracting and detecting the nucleic acid comprises a control unit (1), a transfer unit (2), a sample processing unit (3) and a detection unit (4), wherein the control unit (1) is used for man-machine interaction and storage of system parameters and data, the transfer unit (2) is used for transferring samples and reagents, the sample processing unit (3) is used for purifying nucleic acid in the samples and/or preprocessing the samples, the detection unit (4) is used for detecting the purified samples, the transfer unit (2) comprises 2 porous plate grippers controlled by the control unit (1), and the sample processing unit (3) and the detection unit (4) respectively correspond to one porous plate gripper. The integrated equipment for nucleic acid extraction and detection can improve the operation efficiency of the equipment.
Description
Technical Field
The invention belongs to the technical field of a method for measuring or detecting microorganisms, and particularly relates to integrated equipment for extracting and detecting nucleic acid. .
Background
The invention of application number 201610575345.9 discloses automatic microorganism molecule detection equipment, which comprises a control unit, a transfer unit, a sample pretreatment unit, a nucleic acid purification unit and a detection unit, wherein the control unit is used for man-machine interaction and storage of system parameters and data, the transfer unit is a mechanical arm, the sample pretreatment unit comprises a pretreatment reagent storage area and a separation module, the nucleic acid purification unit is used for purifying nucleic acid in a pretreated sample, and the detection unit is used for detecting the purified sample and obtaining a result. The scheme can realize the original state detection of the sample, but has the following defects: only one porous plate gripper is provided, and the difference between the pretreatment time and the detection time of the sample affects the overall treatment efficiency of the apparatus, and sometimes it is not advantageous to smoothly perform the flow treatment.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides integrated equipment for extracting and detecting nucleic acid, which can improve the operation efficiency of the equipment.
In order to achieve the above object, the technical scheme of the present invention is as follows: the integrated equipment for extracting and detecting the nucleic acid comprises a control unit, a transfer unit, a sample processing unit and a detection unit, wherein the control unit is used for man-machine interaction and storage of system parameters and data, the transfer unit is used for transferring samples and reagents, the sample processing unit is used for purifying nucleic acid in the samples and/or preprocessing the samples, the detection unit is used for detecting the purified samples, the transfer unit comprises 2 porous plate grippers controlled by the control unit, and the sample processing unit and the detection unit respectively correspond to one porous plate gripper.
As an improvement, the sample processing unit and the detection unit are arranged laterally in parallel.
As an improvement, a transit platform for placing the sample is arranged between the sample processing unit and the detection unit.
As a further improvement, the transfer platform comprises a plurality of perforated plate positions.
As a further improvement, limit guardrails are arranged on two sides of the transfer platform.
As an improvement, the detection unit comprises a PCR instrument.
As a further improvement, the detection unit is provided with a multi-well plate outlet.
As a still further improvement, a porous plate pushing mechanism is arranged in the PCR instrument.
The beneficial effects of the invention are as follows: since the sample processing unit and the detection unit respectively correspond to one porous plate gripper, the porous plate gripper of the sample processing unit is responsible for sample processing, and the porous plate gripper of the detection unit is responsible for moving the porous plate into or out of the detection unit. If the detection time is longer, the porous plate gripper of the sample processing unit temporarily stores the porous plate loaded with the sample to be detected in the transfer platform, and the porous plate gripper of the detection unit moves the porous plate gripper of the detection unit into the detection port of the detection unit after the detection unit completes the detection of the previous batch, so that the purposes of time overall and efficiency improvement are achieved. After the detection of the porous plate is completed, the porous plate is pushed out of the PCR instrument by the porous plate pushing mechanism, the porous plate gripper of the detection unit moves to the porous plate outlet, and the experimenter takes away the porous plate from the porous plate outlet. In the prior art, the detected porous plate needs to be manually taken out of the PCR instrument, and once the moving unit fails or the program is improperly set, the moving unit can quickly strike the arm extending into the equipment, so that the arm is damaged. The scheme is characterized in that the porous plate pushing mechanism can automatically lift the porous plate, and the porous plate is moved to the porous plate outlet by the porous plate gripper of the detection unit, so that the processing efficiency is improved, and the system is safer.
Drawings
FIG. 1 is a schematic diagram of the structure of the present invention;
FIG. 2 is a schematic diagram of a transfer platform according to the present invention;
FIG. 3 is a schematic view of a mechanism for pushing out a porous plate in a detection unit according to the present invention.
In the figure: 1. a control unit; 2. a transporting unit; 3. a sample processing unit; 4. a detection unit; 5. a transfer platform; 51. a porous plate; 52. limiting guardrails; 6. a porous plate outlet; 7. a perforated plate pushing mechanism; 8. a partition plate; 81. and a partition window.
Detailed Description
Example 1
As shown in fig. 1, the integrated equipment for nucleic acid extraction and detection comprises a control unit 1, a transfer unit 2, a sample processing unit 3 and a detection unit 4, wherein the control unit 1 is used for man-machine interaction and storage of system parameters and data, the transfer unit 2 is used for transferring samples and reagents, the transfer unit 2 comprises 2 porous plate grippers controlled by the control unit 1, and the sample processing unit 3 and the detection unit 4 respectively correspond to one porous plate gripper. The sample processing unit 3 is used for purifying nucleic acid in a sample and/or preprocessing the sample, the detection unit 4 is used for detecting the purified sample, and the sample processing unit 3 and the detection unit 4 are transversely arranged in parallel.
A partition plate 8 is arranged between the sample processing unit 3 and the detection unit 4, a partition plate window 81 is arranged on the partition plate 8, and a transfer platform 5 for placing the sample is fixed on the partition plate window 81. The structure of the transfer platform 5 is shown in fig. 2, and the transfer platform 5 includes a plurality of perforated plate positions 51, and typically 2 to 4 perforated plate positions 51 are provided. Limiting guardrails 52 are arranged on two sides of the transfer platform 5.
The detection unit 4 comprises a PCR instrument, and a porous plate pushing mechanism 7 is arranged in the PCR instrument. The porous plate pushing mechanism 7 is shown in fig. 3, and the porous plate pushing mechanism 7 is a telescopic base, which is positioned at the bottom of the sample cell of the PCR instrument, so that the porous plate can be conveniently lifted. The detection unit 4 is further provided with a multi-well plate outlet 6.
Claims (4)
1. The integrated equipment for extracting and detecting nucleic acid comprises a control unit (1), a transfer unit (2), a sample processing unit (3) and a detection unit (4), wherein the control unit (1) is used for man-machine interaction and storage of system parameters and data, the transfer unit (2) is used for transferring samples and reagents, the sample processing unit (3) is used for purifying nucleic acid in the samples and/or preprocessing the samples, the detection unit (4) is used for detecting the purified samples, and the detection unit (4) comprises a PCR instrument and is characterized in that: the transfer unit (2) comprises 2 porous plate grippers controlled by the control unit (1), and the sample processing unit (3) and the detection unit (4) respectively correspond to one porous plate gripper; a partition board (8) is arranged between the sample processing unit (3) and the detection unit (4), a partition board window (81) is arranged on the partition board (8), and a transfer platform (5) for placing a sample is fixed on the partition board window (81); the detection unit (4) is provided with a porous plate outlet (6), and a porous plate pushing mechanism (7) is arranged in the PCR instrument.
2. The integrated apparatus for nucleic acid extraction and detection as claimed in claim 1, wherein: the sample processing unit (3) and the detection unit (4) are arranged in parallel transversely.
3. The integrated apparatus for nucleic acid extraction and detection as claimed in claim 1, wherein: the transfer platform (5) comprises a plurality of perforated plate positions (51).
4. The integrated apparatus for nucleic acid extraction and detection as claimed in claim 1, wherein: limiting guardrails (52) are arranged on two sides of the transfer platform (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366498.7A CN106967585B (en) | 2017-05-23 | 2017-05-23 | Integrated device for nucleic acid extraction and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710366498.7A CN106967585B (en) | 2017-05-23 | 2017-05-23 | Integrated device for nucleic acid extraction and detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106967585A CN106967585A (en) | 2017-07-21 |
| CN106967585B true CN106967585B (en) | 2024-03-12 |
Family
ID=59327158
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710366498.7A Active CN106967585B (en) | 2017-05-23 | 2017-05-23 | Integrated device for nucleic acid extraction and detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106967585B (en) |
Citations (13)
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| CN102492603A (en) * | 2011-11-22 | 2012-06-13 | 马庆伟 | Automated instrument for nucleic acid extraction and mass spectrum sample application |
| CN202631350U (en) * | 2012-05-28 | 2012-12-26 | 美特斯工业系统（中国）有限公司 | Low-temperature full-automatic sample sending device of pendulum impact testing machine |
| CN103988064A (en) * | 2011-09-09 | 2014-08-13 | 简.探针公司 | Automated sample handling instrumentation, systems, processes, and methods |
| CN104215030A (en) * | 2014-09-19 | 2014-12-17 | 孙广同 | Children food control device and refrigerator |
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2017
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| US5645801A (en) * | 1993-10-21 | 1997-07-08 | Abbott Laboratories | Device and method for amplifying and detecting target nucleic acids |
| CN1856701A (en) * | 2003-09-24 | 2006-11-01 | 凯纽洛泰克公司 | Device and method for automatically carrying out laboratory procedure steps |
| CN102066950A (en) * | 2008-06-23 | 2011-05-18 | 株式会社日立高新技术 | Nucleic acid analyzer, automatic analyzer, and analysis method |
| EP2198965A1 (en) * | 2008-12-19 | 2010-06-23 | Koninklijke Philips Electronics N.V. | Integrated device for automated simultaneous detection of multiple specific targets using nucleic acid amplification |
| CN103988064A (en) * | 2011-09-09 | 2014-08-13 | 简.探针公司 | Automated sample handling instrumentation, systems, processes, and methods |
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| CN104215030A (en) * | 2014-09-19 | 2014-12-17 | 孙广同 | Children food control device and refrigerator |
| CN105039155A (en) * | 2015-07-29 | 2015-11-11 | 杭州安杰思生物科技有限公司 | Real-time fluorescent quantitative PCR instrument |
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|---|---|
| CN106967585A (en) | 2017-07-21 |
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| TA01 | Transfer of patent application right |
Effective date of registration: 20200909 Address after: 1213-177, 12 / F, building 3, No. 1366, Hongfeng Road, Kangshan street, Huzhou City, Zhejiang Province Applicant after: Original microbial detection technology (Huzhou) Co.,Ltd. Address before: 313008 Zhejiang Province, Huzhou city Wuxing District District Road No. 1188 in the eastern city of Huzhou Metro headquarters free port 11 building H No. 15-16 Applicant before: HUZHOU HIGH-INNO BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20240205 Address after: No.759, East 2nd Ring Road, Wuxing District, Huzhou City, Zhejiang Province Applicant after: HUZHOU TEACHERS College Country or region after: China Address before: Room 1213-177, 12th Floor, Building 3, No. 1366 Hongfeng Road, Kangshan Street, Huzhou City, Zhejiang Province, 313000 Applicant before: Original microbial detection technology (Huzhou) Co.,Ltd. Country or region before: China |
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