CN110157702A - It is a kind of for extracting the acquisition station of coral RNA sample - Google Patents
It is a kind of for extracting the acquisition station of coral RNA sample Download PDFInfo
- Publication number
- CN110157702A CN110157702A CN201910441708.3A CN201910441708A CN110157702A CN 110157702 A CN110157702 A CN 110157702A CN 201910441708 A CN201910441708 A CN 201910441708A CN 110157702 A CN110157702 A CN 110157702A
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- Prior art keywords
- sample
- area
- coral
- extracting
- acquisition station
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Abstract
The invention discloses a kind of for extracting the acquisition station of coral sample, it is related to acquiring station, more particularly to the acquisition station for extracting coral sample, including acquisition platform, acquisition platform upper surface is provided with first area and second area, the first area successively includes sample pipe support area from left to right, zone of origin, sample sterile zones, second area successively includes sample working area from left to right, operating space and suction zones, the first area is sticked with second area and sample pipe support area and sample working area are close, sample sterile zones and suction zones are close, acquisition platform is divided into multiple regions, and function is specified into each region according to the operating habit of operator, it can accelerate the extraction of sample, avoiding sample, exposure generates interference to test result in air for a long time.
Description
Technical field
The present invention relates to acquisition stations, more particularly to the acquisition station for extracting coral RNA sample.
Background technique
The process that RNA sample is extracted is an operating process relatively high to temperature and environmental requirement, in the process of sampling
In, RNA sample is easy to degrade caused by the raising because of temperature, and especially variation during extraction due to temperature is easy to make
At the multigelation of RNA sample, by RNA sample breakdown.
Secondly, RNA sample needs to complete as early as possible during extraction, avoids RNA sample from crossing and be exposed to air for a long time
In, cause the pollution of RNA sample.
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of for extracting the acquisition station of coral RNA sample, the present invention
Used technical solution is:
It is a kind of for extracting the acquisition station of coral RNA sample, including acquisition platform, acquisition platform upper surface is provided with
One region and second area, the first area successively include sample pipe support area, zone of origin, sample sterile zones from left to right, and the
Two regions successively include sample working area, operating space and suction zones from left to right, the first area and second area be sticked and
Sample pipe support area and sample working area are close, and sample sterile zones and suction zones are close.
As a further improvement of the present invention, the sample sterile zones include the disinfection cavity for accommodating thimerosal, described
It is provided with sterilizing lid on disinfection cavity, several sterilization holes for test tool insertion are provided on the sterilizing lid.
As a further improvement of the present invention, the sample working area includes temporary storage cavity and can buckle what temporary chamber lid was closed
Lid, the temporary storage cavity is interior to fill the first dry ice.
As a further improvement of the present invention, first dry ice is provided with N layers, the first dry ice of the n-th layer and
The projection of N- layers of the first dry ice in the vertical direction is mutually perpendicular to, the first dry ice interval setting in same layer.
As a further improvement of the present invention, the operating space includes the operating cavity for accommodating the second dry ice, the behaviour
Make to be provided with operation panel above chamber, be provided on the operation panel several for being connected to the air hole of operating cavity.
As a further improvement of the present invention, the upper surface of the operation panel is gradually raised from edge to center position.
As a further improvement of the present invention, the edge of the operation panel is provided with hangers, and the hangers is connected to operation
On the side wall of chamber.
As a further improvement of the present invention, the suction zones include the water sucting cavity for placing the second dry ice, the suction
It is provided with cover board above water cavity, the cover board upper surface is provided with water absorption layer.
As a further improvement of the present invention, sample pipe support area is provided with test tube bracket.
As a further improvement of the present invention, the zone of origin includes fire source chamber, and the fire source is intracavitary to be provided with fire source, institute
It states and is provided with fireprotection layer between zone of origin and sample sterile zones.
The beneficial effects of the present invention are: acquisition platform is divided into multiple regions, and will according to the operating habit of operator
Function is specified in each region, can accelerate the extraction of RNA sample, avoids RNA sample exposure for a long time in air and to test
As a result interference is generated.
Detailed description of the invention
The present invention is further described with embodiment with reference to the accompanying drawing.
Fig. 1 is structural schematic diagram of the invention.
Specific embodiment
As shown in Figure 1 is a kind of for extracting the acquisition station of coral RNA sample, including acquisition platform 1, flat in acquisition
The upper surface of platform 1 is provided with first area and second area, and wherein first area successively includes sample pipe support area, fire from left to right
Source region and sample sterile zones, second area includes successively from left to right sample working area, operating space and suction zones, wherein the firstth area
Domain and second area are disposed proximate to, and sample pipe support area and sample working area are close, and sample sterile zones and suction zones are close.
This structural order meets the common operating habit of operator, can carry out building library sequencing to RNA as soon as possible, reduce
RNA sample build library be sequenced during because ambient enviroment vulnerable to pollution, it is degradable due to caused by influence.
Specifically sample pipe support area is provided with the test tube bracket 6 for fixing test tube, has been free from RNA in test tube at this time
Sample.Preferably, sample pipe support area is provided with upper end with open sample pipe support chamber, and sample pipe support is intracavitary to be provided with test tube
Bracket 6 is provided with fireprotection layer in the inner wall of sample pipe support chamber.It is provided with zone of origin on the right side of sample pipe support area, the zone of origin
It is interior to be usually fixed with alcolhol burner.Sample sterile zones are provided on the right side of zone of origin.
Sample sterile zones include the disinfection cavity that can accommodate thimerosal, and sterilizing lid 2 is provided on the disinfection cavity, the disinfection cap
2 surface of plate is provided with several sterilization holes 21.After filling thimerosal in disinfection cavity, the instruments such as tweezers, scissors can be inserted into
It carries out disinfection in sterilization hole 21.Sterilizing lid 2 can prevent dust or other sundries from falling into thimerosal, pollute, again
Contact of the thimerosal with air can be reduced, prevent thimerosal from volatilizing.Thimerosal uses alcohol or chloroform under normal conditions.In order to anti-
The heat that only zone of origin generates burns with alcohol, chloroform, adds fireprotection layer between zone of origin and sample sterile zones.
Second area includes sample working area, operating space and suction zones.Wherein sample working area includes temporary storage cavity and can incite somebody to action
The buckle closure 3 that temporary chamber lid is closed.The first dry ice is also filled in temporary storage cavity.Specifically, N layers (N >=1) are provided in temporary storage cavity
First dry ice, wherein n-th layer dry ice is mutually perpendicular to the projection of the first dry ice of N-1 layer in the vertical direction, and in same layer
The first dry ice interval setting.There can be gap between spaced first dry ice, first in conjunction with upper layer or lower layer is dry
Test tube can be firmly fixed in temporary storage cavity by the gap formed between ice prevents test tube from toppling over；It can also be better by test tube
It surrounds, keeps low temperature environment for invisible spectro RNA sample.
Further, aforementioned temporary storage cavity is provided with the buckle closure 3 that can keep in chamber lid conjunction, specific structure are as follows: separate in temporary storage cavity
The side in sample pipe support area is provided with rotation axis 31, is preferably located on lower left in the rotation, before being arranged in the rotation axis 31
Cover board 5 is stated, when the buckle closure that cover board 5 by Unscrew in the horizontal direction, can avoid conventional clamshell formula is opened, in temporary storage cavity
Test tube all exposure in air the case where.In order to guarantee the low-temperature condition being maintained for a long time in temporary storage cavity, in temporary storage cavity
Inner wall on be additionally provided with thermal insulation layer.Thermal insulation layer not only can guarantee that heat exchange did not occurred with the external world for temporary storage cavity, but also can will be not to be lost
Cold air be retained in temporary storage cavity.
Operating space above-mentioned includes operating cavity, and the second dry ice is filled in operating cavity, is provided with behaviour above operating cavity
Make plate 4, the upper surface of the operation panel 4 is gradually raised from edge to center position, in the present embodiment, the upper surface of operation panel 4
The line and horizontal plane of center highest point and edge minimum point form angle, and included angle is 1 °.It is additionally provided on operation panel 4
Several air holes 41, air hole 41 is including being arranged in the inlet end of 4 upper surface of operation panel and going out for 4 lower surface of operation panel being arranged in
Gas end, wherein inlet end face area is greater than outlet side face area.Operating cavity with aforementioned structure can guarantee that second is dry
Ice is that operation panel 4 provides lower temperature, and the moisture in air is made to encounter the operation panel 4 in low-temperature condition and condense out
Water droplet to the edge flowing of operation panel 4, the second dry ice in operating cavity is dropped in by air hole 41 during flowing
On, it is further to be catalyzed the heat absorption of the second dry ice.Inlet end is greater than the air hole 41 of outlet side, facilitates micro liquid because of gravity
It acts on and drips downwards.
Further, it is additionally provided with hangers 42 at the edge of operation panel 4, the effect of the hangers 42 is to be clamped operation panel 4
On the side wall of operating cavity.Operation panel 4 and hangers 42 are preferably metal material.Heat preservation is additionally provided on the inner wall of operating cavity
Layer.
Suction zones above-mentioned include the water sucting cavity for placing the second dry ice, and cover board 5 is provided with above water sucting cavity, lid
The upper surface of plate 5 is provided with water absorption layer, and preferred water absorption layer selects water-absorbing sponge.
First dry ice above-mentioned uses stick-like dry ice, and limit shape, thermal insulation layer above-mentioned do not select foam to the second dry ice, prevents
Fire bed selection fire prevention porcelain tendre.
The above is the preferred embodiment of the present invention, is not constituted a limitation on the scope of protection of the present invention.
Claims (10)
1. a kind of for extracting the acquisition station of coral RNA sample, it is characterised in that: flat in acquisition including acquisition platform (1)
Platform (1) upper surface is provided with first area and second area, and the first area successively includes sample pipe support area, fire from left to right
Source region, sample sterile zones, second area successively include sample working area, operating space and suction zones, firstth area from left to right
Domain is sticked with second area and sample pipe support area and sample working area are close, and sample sterile zones and suction zones are close.
2. according to claim 1 for extracting the acquisition station of coral RNA sample, it is characterised in that: the sample
Sterile zones include the disinfection cavity for accommodating thimerosal, and sterilizing lid (2), the sterilizing lid are provided on the disinfection cavity
(2) several sterilization holes (21) for test tool insertion are provided on.
3. according to claim 1 for extracting the acquisition station of coral RNA sample, it is characterised in that: the sample
Working area includes temporary storage cavity and the buckle closure (3) that can close temporary chamber lid, and the first dry ice is filled in the temporary storage cavity.
4. according to claim 3 for extracting the acquisition station of coral RNA sample, it is characterised in that: described first
Dry ice is provided with N layers, and the projection of the first dry ice and N-1 layers of the first dry ice of the n-th layer in the vertical direction is mutually hung down
Directly, the first dry ice interval setting in same layer.
5. according to claim 4 for extracting the acquisition station of coral RNA sample, it is characterised in that: the operation
Area includes the operating cavity for accommodating the second dry ice, is provided with operation panel (4) above the operating cavity, on the operation panel (4)
It is provided with several for being connected to the air hole (41) of operating cavity.
6. according to claim 5 for extracting the acquisition station of coral RNA sample, it is characterised in that: the operation
The upper surface of plate (4) is gradually raised from edge to center position.
7. according to claim 5 for extracting the acquisition station of coral RNA sample, it is characterised in that: the operation
The edge of plate (4) is provided with hangers (42), and the hangers (42) is connected on the side wall of operating cavity.
8. according to claim 1 for extracting the acquisition station of coral RNA sample, it is characterised in that: the water suction
Area includes the water sucting cavity for placing the second dry ice, is provided with cover board (5) above the water sucting cavity, cover board (5) upper surface
It is provided with water absorption layer.
9. according to claim 1 for extracting the acquisition station of coral RNA sample, it is characterised in that: the sample
Pipe support area is provided with test tube bracket (6).
10. according to claim 1 for extracting the acquisition station of coral RNA sample, it is characterised in that: the fire source
Area includes fire source chamber (7), is provided with fire source in the fire source chamber (7), is provided between the zone of origin and sample sterile zones anti-
Fire bed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910441708.3A CN110157702A (en) | 2019-05-24 | 2019-05-24 | It is a kind of for extracting the acquisition station of coral RNA sample |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910441708.3A CN110157702A (en) | 2019-05-24 | 2019-05-24 | It is a kind of for extracting the acquisition station of coral RNA sample |
Publications (1)
| Publication Number | Publication Date |
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| CN110157702A true CN110157702A (en) | 2019-08-23 |
Family
ID=67632908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910441708.3A Pending CN110157702A (en) | 2019-05-24 | 2019-05-24 | It is a kind of for extracting the acquisition station of coral RNA sample |
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| CN105969763A (en) * | 2016-05-24 | 2016-09-28 | 安徽师范大学 | Extraction method of blueberry RNA |
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| CN108473931A (en) * | 2015-11-02 | 2018-08-31 | 拜奥法尔诊断有限责任公司 | The sample preparation of difficult sample type |
| CN109280611A (en) * | 2018-09-26 | 2019-01-29 | 华蓥市铜堡初级中学 | A kind of expansion RNA extracts kit |
| CN208869582U (en) * | 2018-08-21 | 2019-05-17 | 苏州德思普生物科技有限公司 | The micro-fluidic chip of bacterium total nucleic acid in a kind of extraction human whole blood |
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Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104471074A (en) * | 2012-03-28 | 2015-03-25 | 长角牛疫苗和诊断有限责任公司 | Compositions and methods for the collection and isolation of nucleic acids from biological specimens |
| CN202830011U (en) * | 2012-08-29 | 2013-03-27 | 北京万泰生物药业股份有限公司 | Automated nucleic acid extraction platform |
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| KR20150101420A (en) * | 2014-02-26 | 2015-09-03 | (주)한울티피씨 | device for extracting nucleic acid |
| CN108473931A (en) * | 2015-11-02 | 2018-08-31 | 拜奥法尔诊断有限责任公司 | The sample preparation of difficult sample type |
| CN105969763A (en) * | 2016-05-24 | 2016-09-28 | 安徽师范大学 | Extraction method of blueberry RNA |
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Application publication date: 20190823 |